CN106645127A - Colorimetric kit for detecting ammonia nitrogen in water body and application of colorimetric kit - Google Patents
Colorimetric kit for detecting ammonia nitrogen in water body and application of colorimetric kit Download PDFInfo
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- CN106645127A CN106645127A CN201611268160.XA CN201611268160A CN106645127A CN 106645127 A CN106645127 A CN 106645127A CN 201611268160 A CN201611268160 A CN 201611268160A CN 106645127 A CN106645127 A CN 106645127A
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Abstract
The invention discloses a colorimetric kit for detecting ammonia nitrogen in a water body and application of the colorimetric kit. The colorimetric kit comprises a colorimetric tube, a detection reagent and an ammonia nitrogen standard colorimetric reference card, wherein the detection reagent comprises a reagent I and a reagent II, the reagent I comprises the following components: 20 to 30 parts of N-(1-naphthyl)-ethylenediamine dihydrochloride, 20 to 30 parts of diammonium phosphate, 5 to 10 parts of sodium metavanadate, 12 to 16 parts of salicylic acid, 1 to 4 parts of nitro ammonium phosphate, 1 to 3 parts of inorganic acids and 80 to 100 parts of water; and the reagent II comprises the following components: 30 to 70 parts of diammonium hydrogen citrate, 10 to 30 parts of sodium hypochlorite, 3 to 5 parts of trinitrosalicylic acid, 12 to 16 parts of ethylene diamine tetraacetic acid disodium, and 10 to 20 parts of sodium nitroprusside. The colorimetric kit for detecting the ammonia nitrogen in the water body has the advantages of simplicity, intuitiveness, rapidness, sensitivity, accuracy, low cost, convenience in carrying and the like.
Description
Technical field
The present invention relates to water quality inspection technique field, more particularly to it is a kind of for detecting water body in ammonia nitrogen colorimetric reagent box
And its application.
Background technology
The source of ammonia nitrogen is mainly catabolite, some works of itrogenous organic substance by microbial action in sanitary sewage in water
Superfluous feed and fish, shrimp drain in a large number in industry waste water, such as coking chemical waste water and synthesis ammonia chemical fertilizer factory waste water, agricultural drain, cultivation water
The accumulation of thing and excessively fertilising etc..Ammonia nitrogen mainly exists in two kinds of forms of free ammonia and ammonium salt in water.Cause damaging effect
Mainly free ammonia, its toxicity is more several ten times larger than ammonium salt, and increases with the enhancing of alkalescence.The pH value of ammonia nitrogen toxicity and water and
Water temperature has a substantial connection, ordinary circumstance, and temperature and pH value are higher, and toxicity is stronger.Ammonia nitrogen to fish, the harm of shrimp, similar in appearance to nitrous
Hydrochlorate.Therefore, the content for controlling ammonia nitrogen in water is very necessary, contributes to evaluation water body and is contaminated and " self-cleaning " situation.In water body
Ammonia nitrogen detection method mainly has AAS, chemical titration, high performance liquid chromatography, coulomb analytic approach etc., although these
Detection method differs from one another, but these methods are both needed to for water sample to take back special laboratory, by the operating technology personnelization of specialty
Test and complete, or large-scale, expensive analytical instrument need to be relied on, or sample pretreatment process is complicated, or detect that time-consuming, it is difficult to real
Now to the detection of the simple, intuitive of ammonia nitrogen, rapid sensitive in water body.Ammonia nitrogen color comparison meter be it is a kind of it is portable, for quick detection water
The instrument of middle ammonia nitrogen, is often divided into host machine part and matched reagent part.Can realize that the simple, intuitive to ammonia nitrogen, rapid sensitive are examined
Survey.But error ratio is larger, the degree of accuracy has much room for improvement.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, there is provided a kind of simple, intuitive, rapid sensitive
Accurately, it is with low cost, it is easy to carry for detecting water body in ammonia nitrogen colorimetric reagent box and its application.
To solve above-mentioned technical problem, the present invention is employed the following technical solutions：
It is a kind of for detecting water body in ammonia nitrogen colorimetric reagent box, including colorimetric cylinder, detection reagent and ammonia nitrogen standard colorimetric
To picture ID, the detection reagent includes reagent one and reagent two, and the reagent one includes following components：N- (1- naphthyls)-second two
20～30 parts of amine hydrochlorate, 20～30 parts of phosphoric acid diamines, 5～10 parts of sodium metavanadate, 12～16 parts of salicylic acids, nitro phosphorus ammonium 1～4
80～100 parts of part, 1～3 part of inorganic acid and water；The reagent two includes following components：30～70 parts of citric acid hydrogen diamine, 10～
30 parts of sodium hypochlorite, 3～5 parts of trinitro- salicylic acids, 12～16 parts of disodium ethylene diamine tetraacetates, 10～20 parts of nitroso iron cyanidings
Sodium.
It is above-mentioned for detecting water body in ammonia nitrogen colorimetric reagent box, it is preferred that the reagent one includes following components：
25～28 parts of N- (1- naphthyls)-ethylenediamine-hydrochloride, 25～28 parts of phosphoric acid diamines, 6～8 parts of sodium metavanadate, 14～15 portions of bigcatkin willows
85～95 parts of acid, 3～4 parts of nitro phosphorus ammonium, 2～3 parts of inorganic acid and water；The reagent two includes following components：Hydrogen citrate two
50～60 parts of amine, 15～25 parts of sodium hypochlorite, 3.3～4.5 parts of trinitro- salicylic acids, 14～15 parts of disodium ethylene diamine tetraacetates,
15～28 parts of sodium nitroprussides.
It is above-mentioned for detecting water body in ammonia nitrogen colorimetric reagent box, it is preferred that the reagent one includes following components：
26 parts of N- (1- naphthyls)-ethylenediamine-hydrochloride, 26 parts of phosphoric acid diamines, 7 parts of sodium metavanadate, 15 parts of salicylic acids, 4 parts of nitro phosphorus ammonium,
90 parts of 2 parts of inorganic acid and water；The reagent two includes following components：55 parts of citric acid hydrogen diamine, 20 parts of sodium hypochlorite, trinitro-s
4 parts of salicylic acid, 15 parts of disodium ethylene diamine tetraacetates, 20 parts of sodium nitroprussides.
It is above-mentioned for detecting water body in ammonia nitrogen colorimetric reagent box, it is preferred that the colorimetric cylinder is color-comparison tube.
It is above-mentioned for detecting water body in ammonia nitrogen colorimetric reagent box, it is preferred that the ammonia nitrogen standard colorimetric is to picture ID
The ammonia nitrogen mark of 0.05mg/L, 0.1mg/L, 0.2mg/L, 0.4mg/L, 0.6mg/L, 0.8mg/L and 1.0mg/L is respectively by concentration
The reference colour composition that quasi- solution reaction is produced.
As a total inventive concept, the present invention also provide it is a kind of it is above-mentioned for detecting water body in ammonia nitrogen colorimetric examination
The application of agent box, comprises the following steps：
(1) detected water sample is added in colorimetric cylinder, reagent one is added, after close plug is mixed, reagent two is added, close plug shakes
After even, stand；
(2) colorimetric cylinder after step (1) standing is compareed into card with ammonia nitrogen standard colorimetric to be compareed, records result.
It is above-mentioned for detecting water body in ammonia nitrogen colorimetric reagent box application, it is preferred that the detected water sample, reagent one
It is 25: 5: 5 with the volume ratio of reagent two.
Compared with prior art, it is an advantage of the current invention that：
The present invention is disposable reagent, successively detection reagent is dissolved in surveyed water sample when using, you can by remaining
Chlorine comparator measures residual chlorine concentration.In the presence of sodium nitroprusside, ammonia nitrogen is given birth to salicylate and sodium hypochlorite in water body
Into green compounds, colorimetric assay is carried out according to its shade, using N- (1- the naphthyls)-ethylenediamine of certain content in reagent
Hydrochloride so that the sensitivity of reagent detection is higher, the present invention can be carried out to the chlorine residue in laboratory and live actual water sample
Intuitively, quick, portable detection.
Specific embodiment
Below in conjunction with concrete preferred embodiment, the invention will be further described, following examples set forth the present invention's
Specific embodiments, so that the present invention is further illustrated, these embodiments are simply illustrated and do not indicate that the present invention is all
Possibility, the present invention is not limited only to material in these embodiments, reaction condition or parameter.These are without departing from this
The basic conception of invention description, thus these modifications or it is different should all in the range of the present invention is covered.
Embodiment 1：
A kind of present invention for detecting water body in ammonia nitrogen colorimetric reagent box, including the color-comparison tube of 50mL, detection
, to picture ID, detection reagent includes reagent one, reagent two for reagent and ammonia nitrogen standard colorimetric, and reagent one includes following components：N- (1- naphthalenes
Base) 26 parts of-ethylenediamine-hydrochloride, 26 parts of phosphoric acid diamines, 7 parts of sodium metavanadate, 15 parts of salicylic acids, 4 parts of nitro phosphorus ammonium, inorganic acid 2
90 parts of part and water；Reagent two includes following components：55 parts of citric acid hydrogen diamine, 20 parts of sodium hypochlorite, 4 parts of trinitro- salicylic acids,
15 parts of disodium ethylene diamine tetraacetates, 20 parts of sodium nitroprussides.
Ammonia nitrogen standard colorimetric is respectively 0.05mg/L, 0.1mg/L, 0.2mg/L, 0.4mg/L, 0.6mg/ to picture ID by concentration
The reference colour composition that the Ammonia nitrogen standard liquid reaction of L, 0.8mg/L and 1.0mg/L is produced.
The detection of ammonia nitrogen in water sample：25mL detected water samples are added in color-comparison tube, 5mL reagents one, close plug is added
After mixing, 5mL reagents two are added, after close plug shakes up, stood；Colorimetric cylinder after standing is compareed with ammonia nitrogen standard colorimetric and is sticked into
Row control, as a result shows that fluorinion concentration is 0.6mg/L in water sample to be measured.
Embodiment 2：
A kind of present invention for detecting water body in ammonia nitrogen colorimetric reagent box, including the color-comparison tube of 50mL, detection
, to picture ID, detection reagent includes reagent one, reagent two for reagent and ammonia nitrogen standard colorimetric, and reagent one includes following components：N- (1- naphthalenes
Base) 28 parts of-ethylenediamine-hydrochloride, 27 parts of phosphoric acid diamines, 6 parts of sodium metavanadate, 14 parts of salicylic acids, 4 parts of nitro phosphorus ammonium, inorganic acid 2
95 parts of part and water；Reagent two includes following components：50 parts of citric acid hydrogen diamine, 25 parts of sodium hypochlorite, 4 parts of trinitro- salicylic acids,
14 parts of disodium ethylene diamine tetraacetates, 25 parts of sodium nitroprussides.
Ammonia nitrogen standard colorimetric is respectively 0.05mg/L, 0.1mg/L, 0.2mg/L, 0.4mg/L, 0.6mg/ to picture ID by concentration
The reference colour composition that the Ammonia nitrogen standard liquid reaction of L, 0.8mg/L and 1.0mg/L is produced.
The detection of ammonia nitrogen in water sample：25mL detected water samples are added in color-comparison tube, 5mL reagents one, close plug is added
After mixing, 5mL reagents two are added, after close plug shakes up, stood；Colorimetric cylinder after standing is compareed with ammonia nitrogen standard colorimetric and is sticked into
Row control, as a result shows that fluorinion concentration is 0.8mg/L in water sample to be measured.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned enforcement
Example.All technical schemes belonged under thinking of the present invention belong to protection scope of the present invention.It is noted that for the art
Those of ordinary skill for, improvements and modifications under the premise without departing from the principles of the invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. it is a kind of for detecting water body in ammonia nitrogen colorimetric reagent box, including colorimetric cylinder, detection reagent and ammonia nitrogen standard colorimetric pair
Picture ID, it is characterised in that the detection reagent includes reagent one and reagent two, the reagent one includes following components：N- (1- naphthalenes
Base) 20～30 parts of-ethylenediamine-hydrochloride, 20～30 parts of phosphoric acid diamines, 5～10 parts of sodium metavanadate, 12～16 parts of salicylic acids, nitro
80～100 parts of 1～4 part of phosphorus ammonium, 1～3 part of inorganic acid and water；The reagent two includes following components：Citric acid hydrogen diamine 30～
70 parts, 10～30 parts of sodium hypochlorite, 3～5 parts of trinitro- salicylic acids, 12～16 parts of disodium ethylene diamine tetraacetates, 10～20 parts of Asias
The nitro sodium ferricyanide.
2. it is according to claim 1 for detecting water body in ammonia nitrogen colorimetric reagent box, it is characterised in that the reagent
One includes following components：25～28 parts of N- (1- naphthyls)-ethylenediamine-hydrochloride, 25～28 parts of phosphoric acid diamines, sodium metavanadate 6～8
85～95 parts of part, 14～15 parts of salicylic acids, 3～4 parts of nitro phosphorus ammonium, 2～3 parts of inorganic acid and water；The reagent two includes following
Component：50～60 parts of citric acid hydrogen diamine, 15～25 parts of sodium hypochlorite, 3.3～4.5 parts of trinitro- salicylic acids, 14～15 parts of second
Edetate disodium, 15～28 parts of sodium nitroprussides.
3. it is according to claim 2 for detecting water body in ammonia nitrogen colorimetric reagent box, it is characterised in that the reagent
One includes following components：26 parts of N- (1- naphthyls)-ethylenediamine-hydrochloride, 26 parts of phosphoric acid diamines, 7 parts of sodium metavanadate, 15 portions of bigcatkin willows
90 parts of acid, 4 parts of nitro phosphorus ammonium, 2 parts of inorganic acid and water；The reagent two includes following components：55 parts, 20 parts of citric acid hydrogen diamine
4 parts of sodium hypochlorite, trinitro- salicylic acid, 15 parts of disodium ethylene diamine tetraacetates, 20 parts of sodium nitroprussides.
4. it is according to claim 1 for detecting water body in ammonia nitrogen colorimetric reagent box, it is characterised in that the colorimetric
Manage as color-comparison tube.
5. it is according to claim 1 for detecting water body in ammonia nitrogen colorimetric reagent box, it is characterised in that the ammonia nitrogen mark
Quasi- colorimetric control card by concentration be respectively 0.05mg/L, 0.1mg/L, 0.2mg/L, 0.4mg/L, 0.6mg/L, 0.8mg/L and
The reference colour composition that the Ammonia nitrogen standard liquid reaction of 1.0mg/L is produced.
6. it is a kind of as described in any one of Claims 1 to 5 for detecting water body in ammonia nitrogen colorimetric reagent box application, including
Following steps：
(1) detected water sample is added in colorimetric cylinder, reagent one is added, after close plug is mixed, reagent two is added, close plug shakes up
Afterwards, stand；
(2) colorimetric cylinder after step (1) standing is compareed into card with ammonia nitrogen standard colorimetric to be compareed, records result.
7. it is according to claim 6 for detecting water body in ammonia nitrogen colorimetric reagent box application, it is characterised in that it is described
The volume ratio of detected water sample, reagent one and reagent two is 25: 5: 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611268160.XA CN106645127A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit for detecting ammonia nitrogen in water body and application of colorimetric kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611268160.XA CN106645127A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit for detecting ammonia nitrogen in water body and application of colorimetric kit |
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| CN106645127A true CN106645127A (en) | 2017-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611268160.XA Pending CN106645127A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit for detecting ammonia nitrogen in water body and application of colorimetric kit |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323226A (en) * | 2011-06-03 | 2012-01-18 | 河南师范大学 | A kind of method of measuring three nitrogen concentrations in the water |
| CN105115911A (en) * | 2015-09-18 | 2015-12-02 | 清华大学 | Water quality detecting method and micro-fluidic chip special for water quality detecting method |
| CN105987903A (en) * | 2015-03-03 | 2016-10-05 | 温州医科大学 | On-site quick detection kit of ammonia nitrogen in water and preparation method |
-
2016
- 2016-12-31 CN CN201611268160.XA patent/CN106645127A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323226A (en) * | 2011-06-03 | 2012-01-18 | 河南师范大学 | A kind of method of measuring three nitrogen concentrations in the water |
| CN105987903A (en) * | 2015-03-03 | 2016-10-05 | 温州医科大学 | On-site quick detection kit of ammonia nitrogen in water and preparation method |
| CN105115911A (en) * | 2015-09-18 | 2015-12-02 | 清华大学 | Water quality detecting method and micro-fluidic chip special for water quality detecting method |
Non-Patent Citations (1)
| Title |
|---|
| 丁恒如: "《水中有机物及吸附处理》", 30 June 2016 * |
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