CN104089956A - Quick water iodine testing kit and testing method thereof - Google Patents
Quick water iodine testing kit and testing method thereof Download PDFInfo
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- CN104089956A CN104089956A CN201410345706.1A CN201410345706A CN104089956A CN 104089956 A CN104089956 A CN 104089956A CN 201410345706 A CN201410345706 A CN 201410345706A CN 104089956 A CN104089956 A CN 104089956A
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Abstract
The invention discloses a quick water iodine testing kit. A color developer reagent bottle, a composite oxidizer reagent bottle, a buffer reagent bottle and a shade guide are arranged in a kit body, wherein colors obtained from reaction of known water iodine with different iodine concentrations for 90s serve as color codes, and the shade guide is formed by the color codes. A testing method of the quick water iodine testing kit comprises the following steps: (A) putting a to-be-detected water sample into a test tube, wherein the amount of the water sample is 2ml; (B) dropwise adding a buffer 2 into the test tube by using a drip tube immediately; (C) dropwise adding a color developer 2 into the test tube by using the drip tube, and uniformly mixing; (D) dropwise adding a composite oxidizer 2 into the test tube by using the drip tube, and uniformly mixing; and (E) when the reaction lasts for 90s, comparing the color of a solution in the test tube with the color of the shade guide to determine the concentration of the solution, wherein white paper is used as a background. The quick water iodine testing kit and the testing method thereof have the advantages of quickness in detection, simplicity and convenience in operation, economy, high efficiency, high sensitivity and good stability, and are suitable for on-site operation; and requirements on operating conditions are not limited, and the problem about large-scale on-site detection in water iodine screening or water iodine general inspection is solved.
Description
Technical field
The present invention relates to water iodine detection technique field, be specifically related to the quick test kit of a kind of water iodine and method of testing thereof.
Background technology
In water, the detection of iodine is the conventional sense work of prevention and control of iodine deficient disease, is to understand the requisite test item of IDD (Iodine deficiency disorders) external environment.Existing detection method adopts the arsenic cerium Catalytic Spectrophotometry of the detection method > > of iodine compound in < < Drinking Water, the method reagent type is many, detecting step is loaded down with trivial details, operation each several part vulnerable to pollution, workload is large, time-consuming, and temperature and time on this method impact greatly, need strict control operation condition, be suitable for professional Shui Dian testing laboratory.If carry out the examination of large-scale water iodine or census operations, be badly in need of to find a kind of water iodine method for quick of easy and simple to handle, highly sensitive and applicable execute-in-place.
Summary of the invention
To the object of the invention is the technical matters existing in prior art in order solving, a kind of quick test kit of water iodine of easy and simple to handle, highly sensitive and applicable execute-in-place to be provided.
Another object of the present invention is for the method for testing of the quick test kit of a kind of water iodine is provided.
In order to achieve the above object, the present invention is by the following technical solutions: the quick test kit of a kind of water iodine, in box body, be provided with color-developer reagent bottle, composite oxidant reagent bottle, buffering agent reagent bottle and color board, by the water Iod R of known different levels of iodine, the color after 90 seconds forms as colour code described color board.
Further, the colour code on described color board comprises the colour code of the iodine concentration water Iod R of 20 μ g/L, 50 μ g/L, 150 μ g/L, 200 μ g/L, 300 μ g/L and 400 μ g/L.
Further, described color-developer reagent bottle is that in 1 and color-developer reagent bottle, developer is that concentration is the tetramethyl benzidine of 2-10% and the mixed solution of the Macrogol 6000 that concentration is 2-10%.
Further, described composite oxidant reagent bottle is that in 1 and composite oxidant reagent bottle, composite oxidant is that concentration is the Peracetic acid of 2-10% and the mixed solution of the hydrogen peroxide that concentration is 2-10%.
Further, described buffering agent reagent bottle is that in 1 and buffering agent reagent bottle, buffering agent is sodium acetate-acetic acid buffer solution.
A method of testing for the quick test kit of water iodine, comprises the following steps: A, get water sample 2ml to be detected in test tube; B, with dropper, add immediately 2 of buffering agents in test tube; C, with dropper, add 2 of developers in test tube, mix; D, with dropper, add 2 of composite oxidants in test tube, mix; E, take blank sheet of paper as background, room temperature reaction in the time of 90 seconds in vitro the color of solution contrast with color board color, determine solution concentration.
A method of testing for the quick test kit of water iodine, comprises the following steps: a, get each 2ml of standard series iodine solution in test tube; B, add 50 μ l buffering agents; C, add 150 μ l developers, mix; D, add composite oxidant 50 μ l, mix; E, accurate timing, in the time of 90 seconds, are made reference with water, and spectrophotometer is colorimetric under 650nm condition, records absorbance, drawing standard curve; F, get water sample 2ml to be measured, method of operating, as above-mentioned standard series iodine solution processing mode, is searched its corresponding concentration according to its absorbance on typical curve.
Ultimate principle of the present invention is: under acid condition, catalysis of iodine tetramethyl benzidine and Peracetic acid or hydrogen peroxide generation redox reaction in water, produce coloring matter and develop the color, within the scope of finite concentration, the concentration of iodine and be intensity of colour and be directly proportional, makes color board colorimetric or under 650nm condition, reads absorbance judged result.
The relative prior art of the present invention has following beneficial effect: the present invention adopts tetramethyl benzidine development process make water iodine quick detection kit and by the quick test kit of water iodine, water iodine detected, with respect to existing detection method to the strict demand of testing conditions and the deficiency that can not carry out field experiment, that the present invention detects is quick, easy and simple to handle, economical and efficient, highly sensitive, have good stability, operating conditions is required not limit and applicable execute-in-place, solved the extensive Site Detection problem of the examination of water iodine or generaI investigation.The present invention does not need instrument and equipment, and scene can operate, and sampling amount is little, simple to operate, convenient and swift, time saving and energy saving, cost is lower, has good economic benefit and application prospect.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
The quick test kit of a kind of water iodine, in box body, be provided with color-developer reagent bottle, composite oxidant reagent bottle, buffering agent reagent bottle and color board, color-developer reagent bottle is that in 1 and color-developer reagent bottle, developer is that concentration is the tetramethyl benzidine of 2-10% and the mixed solution of the Macrogol 6000 that concentration is 2-10%, composite oxidant reagent bottle is that in 1 and composite oxidant reagent bottle, composite oxidant is that concentration is the Peracetic acid of 2-10% and the mixed solution of the hydrogen peroxide that concentration is 2-10%, buffering agent reagent bottle is that in 1 and buffering agent reagent bottle, buffering agent is sodium acetate-acetic acid buffer solution, by the water Iod R of known different levels of iodine, the color after 90 seconds forms as colour code color board, colour code on color board comprises 20 μ g/L, 50 μ g/L, 150 μ g/L, 200 μ g/L, the colour code of the iodine concentration water Iod R of 300 μ g/L and 400 μ g/L.
The quick test kit of water iodine of the present invention can quantitatively detect and half-quantitative detection, and half-quantitative detection method comprises following operation steps: A, gets water sample 2ml to be detected in test tube; B, with dropper, add immediately 2 of buffering agents in test tube; C, with dropper, add 2 of developers in test tube, mix; D, with dropper, add 2 of composite oxidants in test tube, mix; E, take blank sheet of paper as background, room temperature reaction in the time of 90 seconds in vitro the color of solution contrast with color board color, determine solution concentration.
When the quick test kit of water iodine of the present invention quantitatively detects, comprise following operation steps: a, get each 2ml of standard series iodine solution in test tube; B, add 50 μ l buffering agents; C, add 150 μ l developers, mix; D, add composite oxidant 50 μ l, mix; E, accurate timing, in the time of 90 seconds, are made reference with water, and spectrophotometer is colorimetric under 650nm condition, records absorbance, drawing standard curve; F, get water sample 2ml to be measured, method of operating, as above-mentioned standard series iodine solution processing mode, is searched its corresponding concentration according to its absorbance on typical curve.
Method validation:
1. the foundation of detection method: national standard method is according to the detection method of iodine compound in People's Republic of China's GB Drinking Water, and the present invention adopts the method for testing (half-quantitative detection method) of the quick test kit of water iodine.
2. Verification Project: (1) control experiment; (2) recovery experiment; (3) field experiment.
(1) control experiment: national standard method and detection method of the present invention are for the comparison of low concentration water sample testing result, for water iodine concentration, be less than the following low concentration water sample of 20 μ g/L, adopt national standard method and detection method of the present invention (half-quantitative detection method), do altogether 6 batches, do parallel testing for every batch, the difference that compares national standard method and detection method measurement result of the present invention, as shown in Table 1:
Table one:
Table one result shows, national standard method and detection method no difference of science of statistics of the present invention.
(2) recovery experiment: get respectively 6 parts of natural water-likes, every part adds 50 μ g iodine standard solutions, and by detection method of the present invention (half-quantitative detection method) colorimetric estimation, result shows that recovery of standard addition is at 98.3%-104.2%.
(3) field experiment: in order to verify detection method fast detecting of the present invention and field experiment ability, in Liu Gu town, Fuping County, Shaanxi Province, Liu market town, three Gong20 villages, township, Dao Xian township carry out field experiment.
1. field by using detection method of the present invention (half-quantitative detection method) detects 20 parts of water samples, according to color board color, testing result is carried out to record.
2. gather 20 parts of identical water samples, send laboratory to adopt national standard method to detect.
3. the testing result of two kinds of detection methods is compared.
Result shows, detection method of the present invention (half-quantitative detection method) and national standard method testing result no difference of science of statistics, for the detection of high iodine water, the detection method of the quick test kit of water iodine of the present invention quick and precisely, rate of accuracy reached 100%.
Claims (7)
1. the quick test kit of water iodine, it is characterized in that: in box body, be provided with color-developer reagent bottle, composite oxidant reagent bottle, buffering agent reagent bottle and color board, by the water Iod R of known different levels of iodine, the color after 90 seconds forms as colour code described color board.
2. the quick test kit of a kind of water iodine according to claim 1, is characterized in that: the colour code on described color board comprises the colour code of the iodine concentration water Iod R of 20 μ g/L, 50 μ g/L, 150 μ g/L, 200 μ g/L, 300 μ g/L and 400 μ g/L.
3. the quick test kit of a kind of water iodine according to claim 1, is characterized in that: described color-developer reagent bottle is that in 1 and color-developer reagent bottle, developer is that concentration is the tetramethyl benzidine of 2-10% and the mixed solution of the Macrogol 6000 that concentration is 2-10%.
4. the quick test kit of a kind of water iodine according to claim 1, is characterized in that: described composite oxidant reagent bottle is that in 1 and composite oxidant reagent bottle, composite oxidant is that concentration is the Peracetic acid of 2-10% and the mixed solution of the hydrogen peroxide that concentration is 2-10%.
5. the quick test kit of a kind of water iodine according to claim 1, is characterized in that: described buffering agent reagent bottle is that in 1 and buffering agent reagent bottle, buffering agent is sodium acetate-acetic acid buffer solution.
6. the method for testing of the quick test kit of a kind of water iodine according to claim 1, is characterized in that: comprise the following steps:
A, get water sample 2ml to be detected in test tube;
B, with dropper, add immediately 2 of buffering agents in test tube;
C, with dropper, add 2 of developers in test tube, mix;
D, with dropper, add 2 of composite oxidants in test tube, mix;
E, take blank sheet of paper as background, room temperature reaction in the time of 90 seconds in vitro the color of solution contrast with color board color, determine solution concentration.
7. the method for testing of the quick test kit of a kind of water iodine according to claim 1, is characterized in that: comprise the following steps:
A, get each 2ml of standard series iodine solution in test tube;
B, add 50 μ l buffering agents;
C, add 150 μ l developers, mix;
D, add composite oxidant 50 μ l, mix;
E, accurate timing, in the time of 90 seconds, are made reference with water, and spectrophotometer is colorimetric under 650nm condition, records absorbance, drawing standard curve;
F, get water sample 2ml to be measured, method of operating, as above-mentioned standard series iodine solution processing mode, is searched its corresponding concentration according to its absorbance on typical curve.
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| CN201410345706.1A CN104089956A (en) | 2014-07-21 | 2014-07-21 | Quick water iodine testing kit and testing method thereof |
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| CN201410345706.1A CN104089956A (en) | 2014-07-21 | 2014-07-21 | Quick water iodine testing kit and testing method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107328769A (en) * | 2017-08-18 | 2017-11-07 | 吉林省富生医疗器械有限公司 | Calcium, magnesium quick detection kit and its detection method in a kind of blood dialysis solution |
| CN107831161A (en) * | 2017-12-06 | 2018-03-23 | 广东轻工职业技术学院 | Small molecule containing acetate is used as the new application of catalyst as class peroxidase |
| CN113884483A (en) * | 2020-07-04 | 2022-01-04 | 合肥谱尼测试科技有限公司 | Rapid detection kit for content of iodine in table salt |
| CN113959822A (en) * | 2021-10-29 | 2022-01-21 | 珠海市丽拓生物科技股份有限公司 | Diluent and oxidant for determining urine iodine content by peroxyacetic acid oxidation method and application |
| CN114509429A (en) * | 2021-12-20 | 2022-05-17 | 苏州百源基因技术有限公司 | Urine iodine detection kit and use method thereof |
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| CN1811394A (en) * | 2006-03-07 | 2006-08-02 | 北京中生金域诊断技术有限公司 | Urine iodine test reagent kit |
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2014
- 2014-07-21 CN CN201410345706.1A patent/CN104089956A/en active Pending
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| CN1811394A (en) * | 2006-03-07 | 2006-08-02 | 北京中生金域诊断技术有限公司 | Urine iodine test reagent kit |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107328769A (en) * | 2017-08-18 | 2017-11-07 | 吉林省富生医疗器械有限公司 | Calcium, magnesium quick detection kit and its detection method in a kind of blood dialysis solution |
| CN107831161A (en) * | 2017-12-06 | 2018-03-23 | 广东轻工职业技术学院 | Small molecule containing acetate is used as the new application of catalyst as class peroxidase |
| CN113884483A (en) * | 2020-07-04 | 2022-01-04 | 合肥谱尼测试科技有限公司 | Rapid detection kit for content of iodine in table salt |
| CN113959822A (en) * | 2021-10-29 | 2022-01-21 | 珠海市丽拓生物科技股份有限公司 | Diluent and oxidant for determining urine iodine content by peroxyacetic acid oxidation method and application |
| CN113959822B (en) * | 2021-10-29 | 2024-02-20 | 珠海市丽拓生物科技股份有限公司 | Diluent and oxidant for measuring iodine content in urine by peroxyacetic acid oxidation method and application |
| CN114509429A (en) * | 2021-12-20 | 2022-05-17 | 苏州百源基因技术有限公司 | Urine iodine detection kit and use method thereof |
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