CN104359900B - Kit for quickly detecting content of proline in honey - Google Patents
Kit for quickly detecting content of proline in honey Download PDFInfo
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- CN104359900B CN104359900B CN201410619143.0A CN201410619143A CN104359900B CN 104359900 B CN104359900 B CN 104359900B CN 201410619143 A CN201410619143 A CN 201410619143A CN 104359900 B CN104359900 B CN 104359900B
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- proline
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Abstract
The invention discloses a kit for quickly detecting content of proline in honey and a using method of the kit. The using method comprises the following steps: (1) preparing a sample, namely accurately weighing and adding 2.5g of honey sample into a small beaker, adding 10mL of distilled water, uniformly mixing the distilled water and the honey sample to obtain a mixture, transferring the mixture to a 50mL volumetric flask after uniformly stirring by virtue of a glass rod, adding distilled water to make up to volume, and uniformly shaking; (2) developing color, namely taking and adding 1.00ml of a to-be-detected sample into a graduated test tube with a stopper, then, adding 1.0mL of a reagent I into the test tube, screwing up a bottle cover, putting the graduated test tube in boiling water and heating for 15 minutes, then, immediately taking out, and adding 5mL of a regent II to develop color for 5 minutes; (3) detecting, namely comparing the color of a developed solution with the color of a color chart to determine the content of proline. According to the kit for quickly detecting the content of proline in honey disclosed by the invention, the color developing system is stable and quick, the operation is simple and convenient, and the kit is suitable for quickly detecting the content of the proline in the honey on the spot.
Description
Technical field
Present invention relates particularly to the test kit of proline content and its using method in a kind of quick detection Mel, belong to food
Quality safety detection technology field.
Background technology
Although containing a variety of aminoacid in Mel, however only proline could as the index of measurement honey quality it
One.In Mel, the content of proline is more taller than total phenols and total flavones, and the content of proline and phenolic compound concentration
It is directly proportional.Therefore, proline not only has non-oxidizability, can be used to weigh the non-oxidizability of Mel, and can also be inspection
Surveying honey quality index provides scientific basis.In detection Mel, the method for proline content mainly has spectrophotometric both at home and abroad at present
Method and chromatography.Although above method is relatively accurate, take, and higher to the technical requirements of equipment and testing staff it is impossible to
It is used widely in department of basic unit.Therefore, the basis of present invention proline content detection technique research in forefathers are to Mel
On, by optimizing the concentration of each constituent and volume in color development system, it is prepared for test kit and the dried meat ammonia comprising multiple color
Sour standard color comparison card a kind of carries out simple, quick method for measuring it is intended to set up to the proline content in Mel.
Content of the invention
The purpose of the present invention is to be to provide the test kit of proline content and its user in a kind of quick detection Mel
Method, live can complete the quick mensure to proline in Mel, have color development system used stable, easy and simple to handle, quick
Feature.
For achieving the above object, the technical scheme is that：
In a kind of quick detection Mel, the test kit of proline content, is made up of reagent I and reagent II；Described reagent I by
1,2,3-indantrione monohydrate, formic acid and ethylene glycol monomethyl ether composition, described reagent II is made up of acetone.
In described reagent I, the concentration of 1,2,3-indantrione monohydrate is 2wt.%, and the volume ratio of formic acid and ethylene glycol monomethyl ether is 1:1.
In quick detection Mel, the using method of the test kit of proline content, comprises the following steps：
（1）The preparation of sample：Weigh 2.5g honey sample in small beaker, after adding 10mL distilled water to stir evenly, use glass
Rod is proceeded to the volumetric flask of 50mL, plus distilled water is settled to scale, shakes up；
（2）Colour developing：Take 1.00mL testing sample in tool plug scale test tube, then in test tube, add 1.0mL reagent I,
Stopper bottle cap, be placed in boiling water and take out after heating 15min at once, add 5mL reagent II colour developing 5min.
（3）Detection:After colour developing, the color of solution and the color contrast of colorimetric card, determine the content value of proline.
Above-mentioned steps（2）Described in color comparison tube be 25mL color-comparison tube, caliber 2cm, high 15 cm of pipe;Above-mentioned step
Suddenly（3）Described in colorimetric card, at normal temperatures, a series of proline standard working solution are shown by the step described in above-mentioned
Color, according to obtained color, makes colorimetric card, and colorimetric card corresponding proline content scope is 0-50 (mg/100g).
Beneficial effects of the present invention：The test kit of proline content quick detection and method in the Mel that the present invention develops,
Its distinguishing feature is：1. color development system is stable, by screening, the isopropanol used in national standard method is changed into acetone
Afterwards, the unstable shortcoming of color development system before effectively overcoming, thus substantially increase the accuracy of detection；2. the present invention borrows
Help colorimetric card control test it is not necessary to the instrument and equipment such as spectrophotometer, easy and simple to handle, it is easy to Site Detection；3. this method is fast
Fast, easy and simple to handle.The method is suitable for the proline content in field quick detection Mel, is quickly to judge honey product quality
One of important evidence of level.
Specific embodiment：
Embodiment 1：
The manufacture method of colorimetric card
（1）The preparation of standard solution：Accurately draw 25 μ g/mL proline standard solution 0,0.2,0.4,0.6,0.8,
1.0mL (content being equivalent to proline in Mel is respectively 0,10,20,30,40,50 mg/100g) tries to six brace plug scales
Guan Zhong, plus distilled water supplies volume to lmL;
（2）Colour developing：Add 1.0mL reagent I in test tube, stopper bottle cap, be placed in boiling water and take after heating 15min at once
Go out, add 5mL reagent II colour developing 5min.According to obtained color, make colorimetric card, the content range of the corresponding proline of colorimetric card
For 0-50 (mg/100g).
Actual sample measures
（1）The preparation of sample：Weigh 2.5g honey of lungan flowers in small beaker, after adding 10mL distilled water to stir evenly, use Glass rod
Proceeded to the volumetric flask of 50mL, plus distilled water is settled to scale, shakes up；
（2）Colour developing：Take 1.00mL testing sample in tool plug scale test tube, then in test tube, add 1.0mL reagent I,
Stopper bottle cap, be placed in boiling water and take out after heating 15min at once, add 5mL reagent II colour developing 5min;
（3）Detection:The color contrast of the color of solution and colorimetric card after colour developing, its color is between proline content
Between color and luster for 10 (mg/100g) and 20 (mg/100g), therefore determine that the proline content in honey sample is 15 (mg/
100g).
Embodiment 2：
The manufacture method of colorimetric card（With example 1）
Actual sample measures
（1）The preparation of sample：Weigh 2.5g Osmanthus forrestii honey in small beaker, after adding 10mL distilled water to stir evenly, use glass
Rod is proceeded to the volumetric flask of 50mL, plus distilled water is settled to scale, shakes up；
（2）Colour developing：Take 1.00mL testing sample in tool plug scale test tube, then in test tube, add 1.0mL reagent I,
Stopper bottle cap, be placed in boiling water and take out after heating 15min at once, add 5mL reagent II colour developing 5min;
（3）Detection:The color contrast of the color of solution and colorimetric card after colour developing, its color and proline content are
The color and luster of 40 (mg/100g) is close, therefore determines that the proline content in honey sample is 40 (mg/100g).
Claims (4)
1. in a kind of quick detection Mel proline content test kit it is characterised in that：It is made up of reagent I and reagent II；Institute
State reagent I to be made up of 1,2,3-indantrione monohydrate, formic acid and ethylene glycol monomethyl ether, described reagent II is made up of acetone；
In described reagent I, the concentration of 1,2,3-indantrione monohydrate is 2wt.%, and the volume ratio of formic acid and ethylene glycol monomethyl ether is 1:1.
2. in a kind of quick detection Mel as claimed in claim 1 the test kit of proline content using method, its feature
It is to comprise the following steps：
（1）The preparation of sample：Weigh 2.5g honey sample in small beaker, after adding 10mL distilled water to stir evenly, will with Glass rod
It proceeds to the volumetric flask of 50mL, plus distilled water is settled to scale, shakes up；
（2）Colour developing：Take 1.00mL testing sample in tool plug scale test tube, then in test tube, add 1.0mL reagent I, stopper
Bottle cap, is placed in boiling water and takes out after heating 15min at once, adds 5mL reagent II colour developing 5min；
（3）Detection:After colour developing, the color of solution and the color contrast of colorimetric card, determine the content value of proline.
3. in a kind of quick detection Mel according to claim 2 the test kit of proline content using method, it is special
Levy and be：Color comparison tube is 25mL color-comparison tube, caliber 2cm, high 15 cm of pipe.
4. in a kind of quick detection Mel according to claim 2 the test kit of proline content using method, it is special
Levy and be：The preparation method of described colorimetric card is at normal temperatures, and a series of proline standard working solution are pressed in claim 2
Described step colour developing, according to obtained color, makes colorimetric card, and colorimetric card corresponding proline content scope is 0-50 mg/
100g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410619143.0A CN104359900B (en) | 2014-11-07 | 2014-11-07 | Kit for quickly detecting content of proline in honey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410619143.0A CN104359900B (en) | 2014-11-07 | 2014-11-07 | Kit for quickly detecting content of proline in honey |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104359900A CN104359900A (en) | 2015-02-18 |
| CN104359900B true CN104359900B (en) | 2017-02-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410619143.0A Expired - Fee Related CN104359900B (en) | 2014-11-07 | 2014-11-07 | Kit for quickly detecting content of proline in honey |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290340A (en) * | 2016-09-14 | 2017-01-04 | 中国烟草总公司广东省公司 | Assay method by the proline content of pesticide infringement Nicotiana tabacum L. |
| CN107179316A (en) * | 2017-07-19 | 2017-09-19 | 山东大学 | A kind of holothurian oral liquid Rapid identification kit |
| CN113884483A (en) * | 2020-07-04 | 2022-01-04 | 合肥谱尼测试科技有限公司 | Rapid detection kit for content of iodine in table salt |
| CN113884455A (en) * | 2021-10-08 | 2022-01-04 | 南京集测生物科技有限公司 | Kit for rapidly detecting proline content and detection method thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002131232A (en) * | 2000-10-24 | 2002-05-09 | Hamamatsu Photonics Kk | Method and kit for detection of hydroxyproline |
| CN1323985A (en) * | 2001-06-29 | 2001-11-28 | 陈建华 | Reagent kit for fast detection of iron content in honey |
| CN100370256C (en) * | 2004-06-11 | 2008-02-20 | 中国兽医药品监察所 | Enzyme-linked immunologic kit for detecting clenbuterol |
| CN103512880B (en) * | 2013-09-18 | 2014-06-11 | 山东大学齐鲁医院 | Joint detection kit for sialic acid and hydroxyproline |
| CN104132939A (en) * | 2014-07-12 | 2014-11-05 | 福建农林大学 | Method for rapidly detecting amylase value in honey |
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2014
- 2014-11-07 CN CN201410619143.0A patent/CN104359900B/en not_active Expired - Fee Related
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| CN104359900A (en) | 2015-02-18 |
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Granted publication date: 20170215 Termination date: 20191107 |