CN106680268A - Colorimetric kit used for detecting fluoride ions in water body and application thereof - Google Patents
Colorimetric kit used for detecting fluoride ions in water body and application thereof Download PDFInfo
- Publication number
- CN106680268A CN106680268A CN201611267568.5A CN201611267568A CN106680268A CN 106680268 A CN106680268 A CN 106680268A CN 201611267568 A CN201611267568 A CN 201611267568A CN 106680268 A CN106680268 A CN 106680268A
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- China
- Prior art keywords
- reagent
- colorimetric
- water body
- detecting
- fluorine ions
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention discloses a colorimetric kit used for detecting fluoride ions in a water body and application thereof. The colorimetric kit comprises a colorimetric tube, detection reagents and a fluoride ion standard colorimetric contrast card, and is characterized in that the detection reagents comprises a first reagent, a second reagent, a third reagent and a fourth reagent; the first reagent is an acetic acid-sodium acetate buffer solution; the second reagent is an alizarin complexometric indicator water solution; the third reagent is N-butyl-1,8-naphthalene amide borate; and the fourth reagent is acetone. The colorimetric kit used for detecting the fluoride ions in the water body has the advantages of being simple, direct, quick, sensitive, accurate, low in cost, convenient to carry and the like.
Description
Technical field
The present invention relates to water quality inspection technique field, more particularly to a kind of colorimetric reagent for detecting fluorine ions in water body
Box and its application.
Background technology
Fluorine is widely present in nature, and fluoride is one of necessary trace element of human body.But while fluorochemical
It is virose, the concentration and intake that fluorion is depended on to human toxicity of fluoride.It is fluorine-containing suitable dense in drinking water
Spend for 0.5~1.0mg/L, high-fluorine water of the long-term drink content more than 1~1.5mg/L can then give people body and bring adverse effect, sternly
The meeting of weight causes dental fluorosis and osteofluorosis.Fluorine in human body detects that fluorion contains in domestic water essentially from drinking water
Measurer is significant.The assay method of fluorion has the chromatography of ions, gas chromatography, fluorimetry, Atomic Absorption point
Light photometry, fluorescence spectrophotometric method etc., although these detection methods differ from one another, but, these methods or need to rely on big
Type, expensive analytical tool, or sample pretreatment process complexity, or detect that time-consuming, it is difficult to realize simply straight to fluorion
Sight, the detection of rapid sensitive.Colorimetry is applied to the relatively low sample of content of fluoride ion, but error ratio is larger, and accuracy needs
Improve.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, there is provided a kind of simple, intuitive, rapid sensitive
Accurately, it is with low cost, the colorimetric reagent box for detecting fluorine ions in water body easy to carry and its application.
To solve above-mentioned technical problem, the present invention is employed the following technical solutions：
A kind of colorimetric reagent box for detecting fluorine ions in water body, including color comparison tube, detectable and fluorion standard
Colorimetric control card, the detectable includes reagent one, reagent two, reagent three and reagent four, and the reagent one is acetic acid-acetic acid
Sodium buffer, the reagent two be Alizarin complexone aqueous solution, the reagent three be N- butyl -1,8- naphthalene amino acid boric acid
Ester, the reagent four is acetone.
The above-mentioned colorimetric reagent box for detecting fluorine ions in water body, it is preferred that the color comparison tube is color-comparison tube.
The above-mentioned colorimetric reagent box for detecting fluorine ions in water body, it is preferred that the fluorion standard colorimetric control
Card by concentration be respectively 0.25mg/L, 0.5mg/L, 0.75mg/L, 1.00mg/L, 1.25mg/L, 1.5mg/L, 1.75mg/L and
The reference colours composition that the fluorion standard solution reaction of 2.0mg/L is produced.
The above-mentioned colorimetric reagent box for detecting fluorine ions in water body, it is preferred that the pH value of Acetic acid-sodium acetate buffer
For 3.5~4.5.
The above-mentioned colorimetric reagent box for detecting fluorine ions in water body, it is preferred that Alizarin complexone aqueous solution
Molar concentration is 0.01M.
Used as a total inventive concept, the present invention also provides a kind of above-mentioned colorimetric for detecting fluorine ions in water body
The application of test kit, comprises the following steps：
(1) detected water sample is added in color comparison tube, Acetic acid-sodium acetate buffer is added and Alizarin complexone is water-soluble
Liquid, after mixing, is slow added into N- butyl -1, and 8- naphthalene amino acid borates after shaking up, add acetone, add deionized water, shake
It is even, stand；
(2) color comparison tube after step (1) standing is compareed into card with fluorion standard colorimetric to be compareed, records result.
The above-mentioned application for detecting the colorimetric reagent box of fluorine ions in water body, it is preferred that the detected water sample, vinegar
The volume ratio of acid-sodium-acetate buffer, Alizarin complexone, N- butyl -1,8- naphthalene amino acid borates, acetone and deionized water
For：25∶5∶2∶5∶10∶3.
The above-mentioned application for detecting the colorimetric reagent box of fluorine ions in water body, it is preferred that the time of repose is 1h.
Compared with prior art, it is an advantage of the current invention that：
Al may be contained in general domestic water3+、Fe2+、Pb2+、Zn2+、Ni2+、Co2+、Cl-、SO4 2-, perchlorate,
Oxalic acid, tartaric acid, citrate etc., during using colorimetry, there is interference to Fluorine Ion Determination, usually using steaming in prior art
The method of evaporating carries out pretreatment, and sample pretreatment process is complicated, and time-consuming for detection.And the present invention for detecting fluorine ions in water body
Increase N- butyl -1 in colorimetric reagent box, 8- naphthalene amino acid boronate reagents, practice have shown that, it is good to the selectivity of fluorion detection,
The impact of other ion pair detections can be avoided；The particularly important is the present invention can be in laboratory and live actual water sample
Fluorion carries out directly perceived, quick, portable detection.
Specific embodiment
Below in conjunction with concrete preferred embodiment, the invention will be further described, following examples set forth the present invention's
Specific embodiments, so that the present invention is further illustrated, these embodiments are simply illustrated and do not indicate that the present invention is all
Probability, the present invention is not limited only to material in these embodiments, reaction condition or parameter.These are without departing from this
The basic conception of invention description, thus these modifications or it is different should all in the range of the present invention is covered.
Embodiment 1：
A kind of colorimetric reagent box for detecting fluorine ions in water body of the present invention, including the color-comparison tube of 50mL, inspection
, to picture ID, detectable includes reagent one, reagent two, reagent three and reagent four, reagent one for test agent and fluorion standard colorimetric
For the Acetic acid-sodium acetate buffer that pH value is 4.0, reagent two is the Alizarin complexone aqueous solution that molar concentration is 0.01M,
Reagent three is N- butyl -1, and 8- naphthalene amino acid borates, reagent four is acetone.
Fluorion standard colorimetric to picture ID by concentration be respectively 0.25mg/L, 0.5mg/L, 0.75mg/L, 1.00mg/L,
The reference colours composition that the fluorion standard solution reaction of 1.25mg/L, 1.5mg/L, 1.75mg/L and 2.0mg/L is produced.
The detection of fluorion in water sample：25mL detected water sample is added in color-comparison tube, 5mL acetic acid-acetic acid is added
Sodium buffer and 2mL Alizarin complexone aqueous solutions, after mixing, are slow added into 5mL N- butyl -1,8- naphthalene amino acid boric acid
Ester, after shaking up, adds 10mL acetone, adds 3mL deionized waters, shakes up, and stands 1h；By color comparison tube and fluorion after standing
Standard colorimetric control card is compareed, and as a result shows that fluorinion concentration is 1.75ppm in water sample to be measured.
Embodiment 2：
A kind of colorimetric reagent box for detecting fluorine ions in water body of the present invention, including the color-comparison tube of 50mL, inspection
, to picture ID, detectable includes reagent one, reagent two, reagent three and reagent four, reagent one for test agent and fluorion standard colorimetric
For the Acetic acid-sodium acetate buffer that pH value is 3.5, reagent two is the Alizarin complexone aqueous solution that molar concentration is 0.01M,
Reagent three is N- butyl -1, and 8- naphthalene amino acid borates, reagent four is acetone.
Fluorion standard colorimetric to picture ID by concentration be respectively 0.25mg/L, 0.5mg/L, 0.75mg/L, 1.00mg/L,
The reference colours composition that the fluorion standard solution reaction of 1.25mg/L and 1.5mg/L is produced.
The detection of fluorion in water sample：25mL detected water sample is added in color-comparison tube, 5mL acetic acid-acetic acid is added
Sodium buffer and 2mL Alizarin complexone aqueous solutions, after mixing, are slow added into 5mL N- butyl -1,8- naphthalene amino acid boric acid
Ester, after shaking up, adds 10mL acetone, adds 3mL deionized waters, shakes up, and stands 1h；By color comparison tube and fluorion after standing
Standard colorimetric control card is compareed, and as a result shows that fluorinion concentration is 2.75ppm in water sample to be measured.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned enforcement
Example.All technical schemes belonged under thinking of the present invention belong to protection scope of the present invention.It is noted that for the art
Those of ordinary skill for, improvements and modifications under the premise without departing from the principles of the invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of colorimetric reagent box for detecting fluorine ions in water body, including color comparison tube, detectable and fluorion standard ratio
Color is to picture ID, it is characterised in that the detectable includes reagent one, reagent two, reagent three and reagent four, and the reagent one is
Acetic acid-sodium acetate buffer, the reagent two be Alizarin complexone aqueous solution, the reagent three be N- butyl -1,8- naphthalenes
Amide borate, the reagent four is acetone.
2. the colorimetric reagent box for detecting fluorine ions in water body according to claim 1, it is characterised in that the colorimetric
Manage as color-comparison tube.
3. the colorimetric reagent box for detecting fluorine ions in water body according to claim 1, it is characterised in that the fluorine from
Substandard colorimetric control card by concentration be respectively 0.25mg/L, 0.5mg/L, 0.75mg/L, 1.00mg/L, 1.25mg/L,
The reference colours composition that the fluorion standard solution reaction of 1.5mg/L, 1.75mg/L and 2.0mg/L is produced.
4. the colorimetric reagent box for detecting fluorine ions in water body according to claim 1, it is characterised in that acetic acid-vinegar
The pH value of sour sodium buffer is 3.5~4.5.
5. the colorimetric reagent box for detecting fluorine ions in water body according to claim 4, it is characterised in that alizarin complexation
The molar concentration of indicator aqueous solution is 0.01M.
6. a kind of application for detecting the colorimetric reagent box of fluorine ions in water body as described in any one of Claims 1 to 5, bag
Include following steps：
(1) detected water sample is added in color comparison tube, Acetic acid-sodium acetate buffer and Alizarin complexone aqueous solution is added,
After mixing, N- butyl -1 is slow added into, 8- naphthalene amino acid borates after shaking up, add acetone, add deionized water, shake up,
Stand；
(2) color comparison tube after step (1) standing is compareed into card with fluorion standard colorimetric to be compareed, records result.
7. the application for detecting the colorimetric reagent box of fluorine ions in water body according to claim 6, it is characterised in that institute
State detected water sample, Acetic acid-sodium acetate buffer, Alizarin complexone, N- butyl -1,8- naphthalene amino acid borates, acetone and go
The volume ratio of ionized water is：25∶5∶2∶5∶10∶3.
8. the application for detecting the colorimetric reagent box of fluorine ions in water body according to claim 7, it is characterised in that institute
Time of repose is stated for 1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611267568.5A CN106680268A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit used for detecting fluoride ions in water body and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611267568.5A CN106680268A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit used for detecting fluoride ions in water body and application thereof |
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|---|---|
| CN106680268A true CN106680268A (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611267568.5A Pending CN106680268A (en) | 2016-12-31 | 2016-12-31 | Colorimetric kit used for detecting fluoride ions in water body and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109238984A (en) * | 2018-09-06 | 2019-01-18 | 哈尔滨医科大学 | It is a kind of for detecting the detection method of content of fluoride ion in trace sample |
| CN110006878A (en) * | 2018-01-04 | 2019-07-12 | 南京江原安迪科正电子研究发展有限公司 | A kind of calibrating Na18The coloration method and kit of fluorinion concentration limit in F injection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2681139Y (en) * | 2004-03-17 | 2005-02-23 | 白莉 | Fluoride colorimetric determination tube |
| CN1955721A (en) * | 2006-07-05 | 2007-05-02 | 天津市起源新技术开发中心 | Method for detecting fluorine content of drinking water and its apparatus |
| CN104677899A (en) * | 2015-02-11 | 2015-06-03 | 中国科学院合肥物质科学研究院 | Fluorinion nanometer colorimetric detection kit and application |
-
2016
- 2016-12-31 CN CN201611267568.5A patent/CN106680268A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2681139Y (en) * | 2004-03-17 | 2005-02-23 | 白莉 | Fluoride colorimetric determination tube |
| CN1955721A (en) * | 2006-07-05 | 2007-05-02 | 天津市起源新技术开发中心 | Method for detecting fluorine content of drinking water and its apparatus |
| CN104677899A (en) * | 2015-02-11 | 2015-06-03 | 中国科学院合肥物质科学研究院 | Fluorinion nanometer colorimetric detection kit and application |
Non-Patent Citations (1)
| Title |
|---|
| 刘淑娟 等: "基于有机硼化合物的氟离子化学探针", 《化学进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006878A (en) * | 2018-01-04 | 2019-07-12 | 南京江原安迪科正电子研究发展有限公司 | A kind of calibrating Na18The coloration method and kit of fluorinion concentration limit in F injection |
| CN109238984A (en) * | 2018-09-06 | 2019-01-18 | 哈尔滨医科大学 | It is a kind of for detecting the detection method of content of fluoride ion in trace sample |
| US11041800B2 (en) | 2018-09-06 | 2021-06-22 | Harbin Medical University | Method for detecting content of fluoride ions in microsamples |
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Application publication date: 20170517 |