CN106645092B - Liquid core waveguide Raman spectrum detection device based on centrifugation - Google Patents
Liquid core waveguide Raman spectrum detection device based on centrifugation Download PDFInfo
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- CN106645092B CN106645092B CN201710103797.1A CN201710103797A CN106645092B CN 106645092 B CN106645092 B CN 106645092B CN 201710103797 A CN201710103797 A CN 201710103797A CN 106645092 B CN106645092 B CN 106645092B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
Abstract
The invention provides a liquid core waveguide Raman spectrum detection device based on centrifugation, which comprises: a bracket provided with a motor; the multi-head spiral disc body is fixed on the bracket and can horizontally rotate around the vertical rotating shaft of the motor, and comprises a disc head and a round table positioned below the disc head; the pan head is provided with a plurality of through holes, and the upper part of each through hole is provided with a positioning groove; the side wall of the round table is provided with spiral channels which are equal in number with the through holes and opposite to the through holes and are used for accommodating the liquid core waveguide tube, and the spiral channels are spirally diverged from top to bottom; the light source probe translation stage is fixed on the bracket and positioned above the multi-head spiral disk body, and comprises a light source probe capable of moving linearly and a positioning mechanism matched with the positioning groove, wherein the positioning mechanism is positioned at the front end of the light source probe and can move forward along with the light source probe and abut against the positioning groove.
Description
Technical Field
The invention relates to a spectrum testing device, in particular to a device for obtaining a liquid core waveguide tube with enough optical path and no bubble by using a centrifugal method and carrying out Raman spectrum detection.
Background
The Teflon AF has a lower refractive index than water, so that when a pipe made of the Teflon AF is filled with water or an aqueous solution, the interface of water and the Teflon AF can satisfy the condition of total reflection, and the light incident at a certain angle has a conductivity similar to that of an optical fiber, forming a so-called liquid core waveguide. The liquid core waveguide has strong light guiding capability, so that a longer optical path can be obtained in the liquid core waveguide, thereby realizing sensitization of various optical detection technologies based on the principles of emission, absorption, fluorescence, scattering and the like, and having good application prospect.
For example, it can be used in the field of raman spectroscopy detection. The Raman spectrum belongs to a molecular vibration spectrum, can obtain information of the influence of groups, chemical bonds and microenvironments on a sample structure from a molecular level, can detect fingerprint information of structures such as protein, nucleic acid, esters and the like of a sample in real time, and can not be interfered by water, so that the Raman spectrum is particularly suitable for detecting biomolecules and has great potential in clinical disease monitoring and early diagnosis.
However, the Teflon AF material is hydrophobic and hydrophilic, and the caliber of the Teflon AF material is thinner and not more than 10mm, so that the hydrophobic surface effect is more serious, and bubbles are easily formed in the Teflon AF material. Once the bubble is formed, the total reflection phenomenon at the bubble position disappears, the light path is broken, and the sensitivity and the stability of the optical measurement are seriously affected. Therefore, before use, the Teflon AF liquid core waveguide needs to be injected with sample and air bubbles are removed.
Two methods for eliminating bubbles in the tube are adopted, one method is to use a centrifugal mode to enable the liquid core waveguide tube to rotate around the shaft, bubble removal is realized by increasing the buoyancy of bubbles of liquid under the centrifugal condition, however, as the centrifugal force is increased, bubbles in the tube are rapidly increased by the pressure of the solution, the volume is continuously compressed, the buoyancy increase is not obvious, and the bubbles are difficult to remove completely due to the obstruction of space; another method is to adopt vacuum degassing method, for example, the Chinese patent application number 201310415459.3 proposes to adopt negative pressure sample injection, and eliminate bubbles in liquid core wave guide tubes while feeding liquid, but each liquid core wave guide tube needs to use a vacuum pump and a plurality of electromagnetic valves in a matching way, and the liquid core wave guide tubes are controlled by a certain program, so that the cost is high and the control is complex.
Because the bubble removal of the liquid core waveguide tube is not easy, the traditional Raman spectrum detection device does not have the function, the traditional method is to prepare the bubble-free liquid core waveguide tube in advance, and comprises the steps of sampling, removing the bubble by adopting the method, detecting whether the bubble is removed, ensuring that the liquid core waveguide tube is taken to the Raman test device for testing after the bubble is free, and the traditional method is troublesome, and the liquid core waveguide tube obtained by the traditional method has short optical path and poor effect in carrying out Raman spectrum detection.
Disclosure of Invention
In order to overcome the defects, the invention provides the liquid core waveguide Raman spectrum detection device based on centrifugation, which realizes liquid core waveguide sample injection and bubble removal by utilizing the centrifugation effect, detects by Raman spectrum, has the dual functions of liquid core waveguide bubble removal and Raman spectrum detection, can obtain a long optical path, and improves the spectrum detection effect.
In order to solve the technical problems, the invention adopts the following technical scheme:
a centrifugal-based liquid core waveguide raman spectrum detection device, comprising:
a bracket provided with a motor;
the multi-head spiral disc body is fixed on the bracket and can horizontally rotate around the vertical rotating shaft of the motor, and comprises a disc head and a round table positioned below the disc head; the pan head is provided with a plurality of through holes, and the upper part of each through hole is provided with a positioning groove; the side wall of the round table is provided with spiral channels which are equal in number with the through holes and opposite to the through holes and are used for accommodating the liquid core waveguide tube, and the spiral channels are spirally diverged from top to bottom;
the light source probe translation stage is fixed on the bracket and positioned above the multi-head spiral disk body, and comprises a light source probe capable of moving linearly and a positioning mechanism matched with the positioning groove, wherein the positioning mechanism is positioned at the front end of the light source probe and can move forward along with the light source probe and abut against the positioning groove.
Further, the spiral channels are uniformly distributed on the side wall of the circular truncated cone.
Further, the upper surface of the pan head is a V-shaped surface with a high edge and a low center, and through holes are uniformly distributed on the V-shaped surface along the circumference.
Further, each through hole comprises a clamping device for fixing the liquid core waveguide tube.
Further, the positioning groove is a conical, spherical, ellipsoidal or cylindrical positioning groove, and the positioning mechanism is a conical, spherical, ellipsoidal or cylindrical positioning mechanism correspondingly.
Further, the spiral channel is in a strip-shaped groove structure.
Further, the spiral divergence direction of the spiral channel is opposite to the rotation direction of the multi-head spiral disc body.
Further, a stop valve is arranged at the lower end of each spiral channel, the stop valve comprises a starting ball, and when the multi-head spiral disc body exceeds a preset rotating speed, the stop valve can be opened under the action of centrifugal force so as to discharge liquid in the liquid core waveguide tube.
Further, the tube wall of the liquid core waveguide tube has air permeability.
Further, the liquid core waveguide tube is fixed in the spiral channel in a clamping or adhesive tape sticking mode.
The beneficial effects of the invention are as follows:
when the device is used, the liquid core waveguide tube is fixed in the spiral channel, the upper end of the liquid core waveguide tube is clamped in the through hole of the pan head, the lower end of the liquid core waveguide tube is connected to the stop valve, a liquid sample is placed in the positioning groove of the pan head, and the liquid slowly enters the liquid core waveguide tube under the action of centrifugal force, so that an analysis channel is finally formed. The device adopts the spiral channel, realizes multiple channels and long optical paths under a smaller volume, and ensures that the whole channel has a larger bending radius; the multi-head spiral disc body adopts the structure of the round table with the small upper part and the big lower part, so that the centrifugal radius is increased along with the increase of the length of the spiral channel, and the centrifugal force of each part of pipe diameter of the liquid core waveguide tube is sequentially increased, thereby ensuring that bubbles in the whole pipeline are effectively removed.
Because the inner diameter of the liquid core waveguide tube is very small, the irradiation light source is required to be aligned in a small range, the device is provided with the positioning mechanism in front of the light source probe, when a certain analysis channel rotates along with the motor to reach an analysis position, the light source probe moves forward along the straight line of the light source probe platform until the positioning mechanism is matched with the positioning groove, and the centering correction of the channel is realized. After analysis is finished, the liquid sample is required to be discharged out of the liquid core waveguide tube, the pipeline is cleaned, when the preset rotating speed is reached, the starting ball starts the stop valve under the action of enough centrifugal force, so that the liquid sample flows out, then cleaning liquid is injected, and the cleaning liquid is input into the liquid core waveguide tube through the centrifugal force and then is discharged out, so that the next analysis is convenient.
Drawings
Fig. 1 is a schematic cross-sectional view of a liquid core waveguide raman spectrum detection apparatus based on centrifugation in an embodiment.
FIG. 2 is a schematic diagram of the position of the light source probe and the pan head.
FIG. 3 is a partial cross-sectional view of a translation stage of a light source probe.
Fig. 4 is a schematic perspective view of a multi-start spiral tray.
Fig. 5 is a cross-sectional view of a tapered detent.
FIG. 6 is a schematic view of a check valve.
Fig. 7 is an exploded view of a check valve assembly.
In the figure: 1-a bracket; 11-a rotating shaft; 12-an electric motor; 2-a light source probe translation stage; 21-a light source probe; 22-a conical positioning mechanism; 3-a multi-head spiral disc body; 31-pan head; 311-a conical positioning groove; 312-through holes; 32-round bench; 321-helical channel; 4-liquid core waveguide tube; 5-a stop valve; 51-start ball; 52-elastic pad.
Detailed Description
In order to make the above features and advantages of the present invention more comprehensible, embodiments accompanied with figures are described in detail below.
The embodiment provides a liquid core waveguide Raman spectrum detection device based on centrifugation, which comprises a bracket 1, a light source probe translation table 2 and a multi-head spiral disc body 3, wherein the light source probe translation table 2 and the multi-head spiral disc body 3 are fixed on the bracket 1, as shown in fig. 1. Wherein, a motor 12 with an upward rotating shaft 11 is arranged on the bracket 1, the multi-head spiral disc body 3 is fixed on the rotating shaft 11, and the multi-head spiral disc body is driven by the motor 12 to horizontally rotate along with the rotating shaft 11.
As shown in fig. 2 and 3, the light source probe translation stage 2 is obliquely fixed above the multi-head spiral disk body 3, and includes a light source probe 21, where the light source probe 21 includes a raman spectrum detection device for carrying out raman spectrum detection on a liquid sample, and can move linearly back and forth along one dimension of the light source probe translation stage 2. A cone-shaped positioning mechanism 22 is arranged at the front end of the light source probe 21 for accurate positioning. In particular applications, the positioning mechanism is not limited to a cone, but may be spherical, cylindrical, ellipsoidal, or other convex shape, and the cone may be conical or pyramidal, etc.
As shown in fig. 2 and 4, the multi-head spiral disk 3 is composed of a circular disk head 31 located above and a circular table 32 located below. The upper surface of the pan head 31 is a V-shaped surface with a high edge and a low center, the center of the V-shaped surface can be provided with a circular plane, and the outline of the shape is not affected, and the key point is that the V-shaped surface comprises an inclined plane, and a plurality of through holes 312 are uniformly distributed on the inclined plane along the circumference, namely, the distances from all the through holes 312 to the center of the V-shaped surface are equal, the distances between any adjacent through holes 312 are equal, the directions of the through holes 312 are perpendicular to the inclined plane where the through holes are located, and the center lines of the through holes and the light source probe 21 can be ensured to be on the same straight line when the through holes are aligned with the inclined plane. In the present embodiment, the number of the through holes 312 is 6, but not limited thereto. The upper port of each through hole 312 is provided with a conical positioning groove 311 which is matched with the conical positioning mechanism 22, and when raman spectrum detection is carried out, the conical positioning mechanism 22 can move forwards and abut against the conical positioning groove 311, so that accurate positioning is realized. In a specific application, the positioning groove is not limited to be conical, and may be spherical, cylindrical, ellipsoidal or other concave shape, and the conical shape may be conical or pyramid shape, etc., which is consistent with the shape of the positioning mechanism and can be matched. In addition, the tapered positioning groove 311 can hold a certain amount of liquid sample, so as to facilitate the sample injection for the liquid core waveguide tube 4. As shown in fig. 5, the through hole 312 has a clamping device, which can clamp and fix the liquid core waveguide 4, and ensure the downward tightness, and prevent the liquid in the conical positioning groove 311 from flowing out through the gap between the through hole 312 and the pipe.
A plurality of spiral channels 321 are uniformly distributed on the side wall of the round table 32, according to the structural characteristics of the round table 32 that the size of the spiral channels 321 is small from top to bottom, the spiral channels 321 gradually spiral and diverge from top to bottom, and the diverging direction is opposite to the rotating direction of the round table 32, namely, if the spiral channels 321 diverge clockwise (or anticlockwise), the rotating direction of the round table 32 is anticlockwise (or clockwise), so that the liquid is ensured to be stressed downwards during rotation and not flow out from the upper end. In the present embodiment, the number of the spiral channels 321 is 6, but not limited thereto. In order to enable the liquid core waveguide tube 4 to be easily installed on the spiral channel 321, the spiral channel 321 is designed into a strip-shaped groove structure, the shape of the groove is not limited, the size is enough to ensure that the liquid core waveguide tube 4 is contained, and the liquid core waveguide tube 4 can be ensured not to fall off in the rotating centrifugal process through the clamping connection or the adhesive tape bonding notch. The shape of the truncated cone 32 can make the spiral channel 321 have the characteristics of liquid flow and centrifugation from top to bottom and from inside to outside, and can realize a long optical path in a small volume. The upper port of the screw channel 321 is opposite to the through hole 312 of the disc head 31 up and down, and a large deviation in the horizontal direction is allowed to exist because of a distance therebetween.
The lower port of the spiral channel 321 is provided with a stop valve 5, and the lower port of the liquid core waveguide 4 is connected to the stop valve 5, as shown in fig. 6 and 7. The stop valve 5 is located below the side wall of the circular table 32 and comprises an actuating ball 51 and an elastic pad 52. When the round table 32 is stationary or the rotation speed is within the preset rotation speed, the stop valve 5 is in a closed state, so that the liquid in the liquid core waveguide tube 4 can be prevented from flowing out. Since the wall of the liquid core waveguide tube 4 has good air permeability (is impermeable to liquid), air bubbles in the tube are discharged through the wall of the tube under the extrusion of the liquid within a preset rotation speed. When the preset rotational speed is exceeded, the actuating ball 51 is forced by a large centrifugal force to open the stop valve 5 by the lower elastic pad 52, thereby discharging the liquid in the pipe under the centrifugal force.
When the device is applied, the upper end of the liquid core waveguide tube 4 is clamped in the through hole 312, the lower end of the liquid core waveguide tube is connected to the stop valve 5, liquid in the conical positioning groove 311 slowly enters the tube under the action of centrifugal force, bubbles in the tube can be discharged through the wall of the tube, an analysis channel is formed when the bubbles are completely discharged, and Raman spectrum detection can be performed on the analysis channel through the light source probe 21. If the bubbles are not completely removed, the liquid core waveguide 4 does not form total reflection, and the light source probe 21 cannot receive the raman spectrum signal, so that it can be judged whether the bubbles are completely removed by the light source probe 21. Since the size of the device is fixed and the mass (or density) of the liquid in the tube is substantially in a range, the liquid is represented by the formula f=mω 2 The centrifugal force is mainly determined by the rotation speed, and the preset speed can be determined after repeated experiments, for example, the preset rotation speed is 500-1000 rpm (revolutions per minute). Therefore, in practical applications, bubbles can be exhausted at a preset speed, and the light source probe 21 is rarely used to determine whether bubbles are exhausted. In order to ensure that the liquid core waveguide tube 4 is used next time, the tube is cleaned after the use is finished, and the cleaning liquid is utilized, so that the cleaning can be finished by adopting the same method.
Two specific examples of applications are listed below:
example 1
5 microliters of 10% ethanol solution was added to the reservoir (i.e., conical detent 311) on the sample tray (i.e., pan head 31), and the sample tray was rotated at 500rpm or more to allow the solution to pass through the reservoir and fill a 10cm long Teflon AF tube (i.e., wick waveguide 4). The Raman signal of the 10% ethanol solution can be measured by the Raman detection probe (namely the light source probe 21), the signal intensity is improved by more than 50 times compared with the intensity measured in a cuvette, and the stability and the repeatability are good, so that the device can effectively realize bubble-free sample injection of liquid in a Teflon AF tube.
Example 2
100. Mu.l of DNA extraction solution was added to a reservoir (i.e., tapered detent 311) on a sample tray (i.e., pan head 31), and the sample tray was rotated at a speed of 500rpm or more so that the solution passed through the reservoir and filled with a 20cm long Teflon AF tube (i.e., wick waveguide 4). The optical fiber receiving probe (namely the light source probe 21) connected with the 260nm LED light source measures the absorption signal of the DNA solution to 260nm light, the signal intensity is improved by more than 10 times compared with the intensity measured in a 1cm cuvette, and the stability repeatability is good, so that the device can effectively realize bubble-free sample injection of liquid in a Teflon AF tube.
Claims (8)
1. A centrifugal-based liquid core waveguide raman spectrum detection device, comprising:
a bracket provided with a motor;
the multi-head spiral disc body is fixed on the bracket and can horizontally rotate around the vertical rotating shaft of the motor, and comprises a disc head and a round table positioned below the disc head; the pan head is provided with a plurality of through holes, and the upper part of each through hole is provided with a positioning groove; the side wall of the round table is provided with spiral channels which are equal in number with the through holes and opposite to the through holes and are used for accommodating the liquid core waveguide, the spiral channels are spirally diverged from top to bottom, the spiral divergence direction of the spiral channels is opposite to the rotation direction of the multi-head spiral disc body, and the pipe wall of the liquid core waveguide has air permeability;
the light source probe translation stage is fixed on the bracket and positioned above the multi-head spiral disk body, and comprises a light source probe capable of moving linearly and a positioning mechanism matched with the positioning groove, wherein the positioning mechanism is positioned at the front end of the light source probe and can move forward along with the light source probe and abut against the positioning groove.
2. The centrifugal based liquid core waveguide raman spectrum detection device according to claim 1, wherein spiral channels are uniformly distributed on the side wall of the circular truncated cone.
3. The centrifugal-based liquid core waveguide Raman spectrum detection device according to claim 1, wherein the upper surface of the pan head is a V-shaped surface with a high edge and a low center, and the through holes are uniformly distributed on the V-shaped surface along the circumference.
4. The centrifugation-based liquid-core waveguide raman spectrum detection apparatus according to claim 1, wherein each through-hole comprises a clamping means for fixing the liquid-core waveguide.
5. The centrifugal-based liquid core waveguide raman spectrum detection device according to claim 1, wherein the positioning groove is a conical, spherical, ellipsoidal, cylindrical positioning groove, and the positioning mechanism is a conical, spherical, ellipsoidal, cylindrical positioning mechanism, respectively.
6. The centrifugal-based liquid core waveguide raman spectrum detection device according to claim 1, wherein the spiral channel is a bar-shaped groove structure.
7. The centrifugal-based liquid core waveguide raman spectrum detection device according to claim 1, wherein a stop valve is provided at a lower port of each spiral channel, the stop valve comprising an actuating ball which opens the stop valve under centrifugal force to discharge liquid in the liquid core waveguide when the multi-headed spiral disc exceeds a predetermined rotational speed.
8. The centrifugal-based liquid core waveguide raman spectrum detection device according to claim 1, wherein the liquid core waveguide is fixed in the spiral channel by means of clamping or adhesive tape sticking.
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4640592A (en) * | 1983-01-22 | 1987-02-03 | Canon Kabushiki Kaisha | Optical display utilizing thermally formed bubble in a liquid core waveguide |
| US5604587A (en) * | 1995-11-16 | 1997-02-18 | World Precision Instruments, Inc. | Long capillary waveguide raman cell |
| WO1999057584A1 (en) * | 1998-05-01 | 1999-11-11 | University Of South Florida | Liquid core waveguide |
| CN1680814A (en) * | 2004-04-09 | 2005-10-12 | 太阳诱电株式会社 | Method of nucleic acid analysis, nucleic acid analysis device and disk for nucleic acid analysis |
| CN101398436A (en) * | 2008-09-27 | 2009-04-01 | 东北大学 | Rotating micro-example auto-introducing device |
| CN101522916A (en) * | 2006-08-02 | 2009-09-02 | 柳在泉 | Thin film chemical analysis apparatus and analysis method using the same |
| CN101806726A (en) * | 2010-03-29 | 2010-08-18 | 浙江大学 | Double-wavelength absorbance detection device for analyzing trace liquid |
| CN103454221A (en) * | 2013-09-12 | 2013-12-18 | 北京吉天仪器有限公司 | Negative-pressure liquid feeding device based on liquid core wave guide pipe |
| CN103822910A (en) * | 2012-11-16 | 2014-05-28 | 福州高意通讯有限公司 | Raman probes for miniature Raman spectrometer |
| CN104614363A (en) * | 2015-01-29 | 2015-05-13 | 上海如海光电科技有限公司 | Raman spectrum testing system based on liquid core waveguide |
| CN105115809A (en) * | 2015-09-11 | 2015-12-02 | 北京本立科技有限公司 | Analysis sample preparation device and analysis sample preparation method |
| CN105259358A (en) * | 2015-11-10 | 2016-01-20 | 中国科学院重庆绿色智能技术研究院 | Multi-channel rotary type Raman spectroscopy detection sample pool device and detecting method thereof |
| CN205091174U (en) * | 2015-09-11 | 2016-03-16 | 北京本立科技有限公司 | Analysis sample preparation device |
| CN106442081A (en) * | 2016-09-30 | 2017-02-22 | 北京本立科技有限公司 | Bubble-free liquid core waveguide tube liquid feeding device based on centrifuging |
| CN206557101U (en) * | 2017-02-24 | 2017-10-13 | 北京本立科技有限公司 | A kind of liquid core waveguide Raman spectrum detecting device based on centrifugation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7444053B2 (en) * | 2003-06-16 | 2008-10-28 | The Regents Of The University Of California | Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity |
| US7574076B2 (en) * | 2005-04-08 | 2009-08-11 | Arryx, Inc. | Apparatus for optically-based sorting within liquid core waveguides |
-
2017
- 2017-02-24 CN CN201710103797.1A patent/CN106645092B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4640592A (en) * | 1983-01-22 | 1987-02-03 | Canon Kabushiki Kaisha | Optical display utilizing thermally formed bubble in a liquid core waveguide |
| US5604587A (en) * | 1995-11-16 | 1997-02-18 | World Precision Instruments, Inc. | Long capillary waveguide raman cell |
| WO1999057584A1 (en) * | 1998-05-01 | 1999-11-11 | University Of South Florida | Liquid core waveguide |
| CN1680814A (en) * | 2004-04-09 | 2005-10-12 | 太阳诱电株式会社 | Method of nucleic acid analysis, nucleic acid analysis device and disk for nucleic acid analysis |
| CN101522916A (en) * | 2006-08-02 | 2009-09-02 | 柳在泉 | Thin film chemical analysis apparatus and analysis method using the same |
| CN101398436A (en) * | 2008-09-27 | 2009-04-01 | 东北大学 | Rotating micro-example auto-introducing device |
| CN101806726A (en) * | 2010-03-29 | 2010-08-18 | 浙江大学 | Double-wavelength absorbance detection device for analyzing trace liquid |
| CN103822910A (en) * | 2012-11-16 | 2014-05-28 | 福州高意通讯有限公司 | Raman probes for miniature Raman spectrometer |
| CN103454221A (en) * | 2013-09-12 | 2013-12-18 | 北京吉天仪器有限公司 | Negative-pressure liquid feeding device based on liquid core wave guide pipe |
| CN104614363A (en) * | 2015-01-29 | 2015-05-13 | 上海如海光电科技有限公司 | Raman spectrum testing system based on liquid core waveguide |
| CN105115809A (en) * | 2015-09-11 | 2015-12-02 | 北京本立科技有限公司 | Analysis sample preparation device and analysis sample preparation method |
| CN205091174U (en) * | 2015-09-11 | 2016-03-16 | 北京本立科技有限公司 | Analysis sample preparation device |
| CN105259358A (en) * | 2015-11-10 | 2016-01-20 | 中国科学院重庆绿色智能技术研究院 | Multi-channel rotary type Raman spectroscopy detection sample pool device and detecting method thereof |
| CN106442081A (en) * | 2016-09-30 | 2017-02-22 | 北京本立科技有限公司 | Bubble-free liquid core waveguide tube liquid feeding device based on centrifuging |
| CN206557101U (en) * | 2017-02-24 | 2017-10-13 | 北京本立科技有限公司 | A kind of liquid core waveguide Raman spectrum detecting device based on centrifugation |
Non-Patent Citations (2)
| Title |
|---|
| 周靖 ; 顾爱平 ; .基于液芯波导激光诱导荧光检测器的缝管自动进样毛细管电泳仪的研制及其在DNA分析中的应用.现代科学仪器.2010,(05),全文. * |
| 杜文斌,方群,方肇伦.基于液芯波导原理的微流控芯片长光程光度检测系统.高等学校化学学报.2004,(04),全文. * |
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