CN106645019A - Method for quick determination of effective phosphorus content in corn for poultry feeding and application of such method - Google Patents
Method for quick determination of effective phosphorus content in corn for poultry feeding and application of such method Download PDFInfo
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- CN106645019A CN106645019A CN201610903622.4A CN201610903622A CN106645019A CN 106645019 A CN106645019 A CN 106645019A CN 201610903622 A CN201610903622 A CN 201610903622A CN 106645019 A CN106645019 A CN 106645019A
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- semen maydiss
- available phosphorus
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 90
- 239000011574 phosphorus Substances 0.000 title claims abstract description 90
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 87
- 238000000034 method Methods 0.000 title claims abstract description 35
- 240000008042 Zea mays Species 0.000 title claims abstract description 33
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 33
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 33
- 235000005822 corn Nutrition 0.000 title claims abstract description 33
- 244000144977 poultry Species 0.000 title claims abstract description 31
- 238000002329 infrared spectrum Methods 0.000 claims abstract description 23
- 241000287828 Gallus gallus Species 0.000 claims abstract description 16
- 235000014786 phosphorus Nutrition 0.000 claims description 96
- 210000000582 semen Anatomy 0.000 claims description 39
- 230000003595 spectral effect Effects 0.000 claims description 19
- 210000003608 fece Anatomy 0.000 claims description 18
- 238000003556 assay Methods 0.000 claims description 14
- 238000001228 spectrum Methods 0.000 claims description 9
- 238000012937 correction Methods 0.000 claims description 7
- 235000005911 diet Nutrition 0.000 claims description 7
- 230000037213 diet Effects 0.000 claims description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000003651 drinking water Substances 0.000 claims description 5
- 235000020188 drinking water Nutrition 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 125000005843 halogen group Chemical group 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 230000000803 paradoxical effect Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 3
- 229910052721 tungsten Inorganic materials 0.000 claims description 3
- 239000010937 tungsten Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 230000009466 transformation Effects 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 20
- 235000013330 chicken meat Nutrition 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 238000003304 gavage Methods 0.000 description 6
- 230000029142 excretion Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000004611 spectroscopical analysis Methods 0.000 description 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000002949 phytic acid Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 206010059410 Faecaluria Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000008979 phosphorus utilization Effects 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
Abstract
The invention relates to the technical field of feed determination, in particular to a method for quick determination of effective phosphorus content in corn for poultry feeding and application of such method. The method includes establishing a quantitative model between a near-infrared spectrum with a characteristic wave band 950-1650 nm in length and effective phosphorus content in corn based on a near-infrared spectrum technology, scanning a near-infrared spectrum with a characteristic wave band of a sample to be determined, and importing the near-infrared spectrum into the quantitative model so as to obtain the effective phosphorus content of the sample to be determined. The method has the advantages that the method is capable of determining the effective phosphorus content in the corn for poultry feeding rapidly and accurately, and the effective phosphorus content is used as a poultry feed preparation index, so that feed mixing accuracy can be guaranteed, feed transformation efficiency is improved effectively, feed waste is reduced, broiler production performance can be given to full play, and breeding cost is reduced.
Description
Technical field
The present invention relates to feedstuff detection technique field, and in particular to available phosphorus content is fast in a kind of feed for poultry Semen Maydiss
Fast assay method and application.
Background technology
Phosphorus is one of mineral element necessary to poultry, and phosphorus wretched insufficiency shows as production performance lowly in feedstuff, and childhood is moved
Thing suffers from ricketss, and adults suffer from malacosiss (or claiming osteoporosis).Poultry to the requirement of phosphorus generally using total phosphorus (TP),
Non-phytate phosphorus (NPP) and available phosphoruss (AP) statement (NRC, 1994；Fowl raising standard, 2004).Previously think, in vegetable raw material
The 1/3 of total phosphorus is NPP, in addition 2/3 phytate phosphorus to be utilized by nonruminant, so be simply equal to NPP having
Effect phosphorus (NRC, 1994).But the research of existing some scholars has shown that, animal can utilize to a certain extent phytate phosphorus, and its
Utilization rate has larger difference because of raw material type difference, and animal is to the difference of phytic acid phosphorus utilization in vegetality feedstuff and examination
Testing the factor such as design, research method, feedstuff and trophic component, the course of processing, analysis method, animal age in days and kind has
Close, and the Non-phytate phosphorus that laboratory is measured can not completely be utilized by animal.Thus total phosphorus and Non-phytate phosphorus all can not be really anti-
The potency of phosphorus in feedstuff is reflected, with the accumulation of research work, available phosphoruss necessarily replace Non-phytate phosphorus to utilize as phosphorus is evaluated
The index of rate.
In consideration of it, research and develop it is a kind of can apply in poultry Feed Manufacturing it is easy, quick, accurately evaluate Semen Maydiss
The method of middle available phosphoruss, for preparation poultry is realized, precisely raising and feedstuff efficient utilization have great importance.
The content of the invention
In order to overcome the defect of prior art, it is an object of the invention to provide available phosphoruss contain in a kind of feed for poultry Semen Maydiss
The rapid assay methods of amount, realize the content of available phosphoruss in quick, Accurate Determining feed for poultry Semen Maydiss, improve feed formulation
Accuracy, reduces phosphorus pollution, saves feed cost.
Meanwhile, the present invention also resides in the rapid assay methods for providing available phosphorus content in a kind of feed for poultry Semen Maydiss in control
Application in poultry Semen Maydiss diet feed processed in terms of phosphorus content.
In order to realize object above, the technical solution adopted in the present invention is：
The rapid assay methods of available phosphorus content in a kind of feed for poultry Semen Maydiss, including following operating procedure：
1) the normal com sample of known available phosphorus content is taken, near infrared spectrum scanning is carried out, collection Semen Maydiss 950～
Spectral information in 1650nm is interval；
2) spectral information is carried out after pretreatment, Applied Chemometrics software is beautiful with the standard of known available phosphorus content
The available phosphorus content of rice sample is associated, and is set up between near infrared spectrum and Semen Maydiss available phosphorus content using partial least square method
Quantitative model；
3) taking corn sample to be measured carries out near infrared spectrum scanning, gather 950～1650nm of Semen Maydiss it is interval in spectrum believe
Breath, spectral information is carried out after pretreatment, by process after corn sample near infrared light spectrum information steps for importing 2 to be measured) build
Vertical quantitative model, draws corn sample available phosphorus content to be measured.
Further, step 1) and step 3) near infrared spectrum scanning light source be mercury lamp；Reference source is halogen tungsten lamp；
950～1100nm of wave-length coverage adopts silicon detector, 110～1650nm of wave-length coverage to adopt diode array detector, wavelength
Precision≤0.3nm, wavelength stability≤0.2nm/a；Scanning circumstance is required：2～40 DEG C of temperature, relative humidity 0～85%.
Further, in order to prevent sample from going mouldy impact spectra collection, the sample for collecting is placed in 4 DEG C of refrigerators
Preserve, before scanning sample, sample was placed on into equilibrium temperature in normal temperature environment in 24 hours in advance, after scanning start 1h is preheated.
Further, step 2) and step 3) in using First derivative spectrograply combine multiplicative scatter correction method near infrared spectrum
Information carries out pretreatment.
Further, step 2) in also include that the estimated performance of quantitative model to setting up is evaluated, evaluating master
There is quantitative model coefficient of determination R2Cal, correction root-mean-square deviation RMESS, checking root-mean-square deviation RMSEP, the checking coefficient of determination
R2Val, number of principal components Ranks, average deviation Bias；Screening obtains R2Cal and R2Val is close 1, RMESS and RMSEP is less and
Closer to quantitative model.
It is above-mentioned to step 2) concrete grammar evaluated of estimated performance of quantitative model set up is to stay one using interaction
Checking, i.e., reject a sample from the normal com sample of known available phosphorus content, and with remaining other samples mathematics is set up
Model, and with the concentration of component to be measured in the disallowable sample of model prediction set up, the prediction effect of model is evaluated.
Wherein R2The capability of fitting of cal reaction models, R2Cal is closer to 1, and the fitness of model is better, and its computing formula is
Wherein：ym- sample actual measured value meansigma methodss；yi- i-th sample actual measured value；- near using what is set up
The predictive value of i-th sample of infrared spectrum quantitative model；
RMSEE computing formula are：
Wherein,- sample actual measured value meansigma methodss.
RMSEP represents the close degree between near-infrared predictive value and actual measured value, and the lower accuracy of numerical value is higher.Its
Computing formula is：
Average deviation is the system deviation of actual measured value and near-infrared predictive value, and its computing formula is：
Wherein：diThe difference of-i-th sample actual measured value and predictive value.
Further, the available phosphorus content in the normal com sample is measured using gavage, used as normal com sample
The known available phosphorus content of product, concrete operation step is：
A：From it is more than body weight 1.8kg, body weight is close, feeding is normal, it is strong raise after be as good as paradoxical reaction, the health sea without odd habit
Blue brown some of cock, single cage is raised, and raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and daily light application time is 16 hours,
Free water, fasting sandstone；
B：Tested cock feeding laying hens in growth period complete feed more than three days, and final stage feeds corn sample to be measured
Afterwards, carry out fasting to empty 48 hours, by drinking-water per chicken daily iron supplement glucose 50g during fasting；
C：Fasting strong corn sample to be measured for raising quality for m (40～50g) after terminating, the continuous excretion for collecting 48 hours
Thing；Can adopt and grow at accent periphery suture 60ml plastic bottle closures in rushing down for tested cock, a circular hole and right is dug in bottle cap face central authorities
The 4 pairs of apertures for claiming, use so that fecaluria Excreta passes through and suture fixed bottle cap, during Excreta is collected, collection excretion of screwing on
The plastic bottle of thing collects Excreta；Or Excreta is collected using collection excrement disk；It is daily during excreta collection to collect several times,
Less than 4 DEG C being stored in immediately after collecting every time, or directly being dried at 60～65 DEG C to constant weight, bottling is sealed up for safekeeping；
D：Tested cock is fed according to the same mode of step B, tested cock fasting is strong after terminating to raise and corn-like to be measured
Product equal quality without phosphorus diet, and collect the Excreta of tested cock 48h according to the same mode of step C；
E：Content m of phosphorus in the Excreta of the 48h for collecting in determination step C respectively1；The excretion of the 48h collected with step D
Thing collects content m of phosphorus2；Quality measurement is content m of the total phosphorus in the corn sample to be measured of m3；Unit of account quality (1g) is treated
Survey the available phosphorus content m of corn sampleAvailable phosphoruss=m3-(m1-m2)/m。
Total phosphorus content in above-mentioned Excreta and corn sample to be measured adopts Yang Sheng《Forage analysis and determination of feeds quality
Technology》Disclosed in method detected.
The rapid assay methods of available phosphorus content are in control poultry Semen Maydiss diet feed in above-mentioned feed for poultry Semen Maydiss
Application in terms of phosphorus content, specially using using said determination method detect feed for poultry Semen Maydiss in available phosphorus content as
Index, according to the demand in poultry diet for phosphorus content, determines the consumption of Semen Maydiss, can effectively improve the conversion effect of phosphorus in daily ration
Rate, reduces daily ration and wastes and phosphorus pollution, reduces feeding cost.
The rapid assay methods of available phosphorus content, using near-infrared spectrum technique, adopt in feed for poultry Semen Maydiss of the present invention
Spectral information in collection 950～1650nm of Semen Maydiss is interval, fast and accurately determines the phosphorus content in Semen Maydiss, and as Semen Maydiss house is fed
Available phosphoruss index during fowl, has the advantages that simple to operate, accuracy is high, can provide for the phosphorus content in feed formulation
Accurately data-guiding, improves the utilization rate of phosphorus in poultry Semen Maydiss daily ration, reduces the pollution of phosphorus, saves feed cost.
Further, near infrared spectrum detection process, the resolution of spectral scan, detection mode, spectral scan model
Enclose, scanning circumstance etc. can affect the accuracy that near infrared spectrum detects, and for different determination samples, its influence
It is also uncertain, especially the preprocess method of spectrum has many kinds, then feed house in detection to improve in the present invention
The accuracy of available phosphorus content in fowl Semen Maydiss, creative selects the Parameter Conditions of spectral scan, and combines First derivative spectrograply
Pretreatment is carried out near infrared spectrum with reference to multiplicative scatter correction method.
Further, during near infrared spectrum scanning, the accuracy of the quantitative model of foundation is determined to a great extent
Determine the accuracy of final measurement result, in order to improve reliability, stability and the dynamic adaptable of quantitative model in the present invention,
Using the available phosphorus content in gavage bioassay standard corn sample.
Specific embodiment
Technical scheme is described in detail below by specific embodiment.
Embodiment
The rapid assay methods of available phosphorus content in a kind of feed for poultry Semen Maydiss, including following operating procedure：
1) 10～20 parts of different normal com samples of available phosphorus content are taken, gavage bioassay standard corn-like is respectively adopted
Phosphorus content in product, used as the available phosphoruss standard content of normal com sample, concrete operation step is：
A：From it is more than body weight 1.8kg, body weight is close, feeding is normal, it is strong raise after be as good as paradoxical reaction, the health sea without odd habit
Blue brown some of cock, single cage is raised, and raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and daily light application time is 16 hours,
Free water, fasting sandstone；
B：Tested cock feeding laying hens in growth period complete feed more than three days, and final stage feeds corn sample to be measured
Afterwards, carry out fasting to empty 48 hours, by drinking-water per chicken daily iron supplement glucose 50g during fasting；
C：Fasting after terminating strong quality of raising be m, 40～50g, corn sample to be measured, the continuous excretion for collecting 48 hours
Thing；Suture 60ml plastic bottle closures at accent periphery are grown using in rushing down for tested cock, a circular hole and symmetrical 4 is dug in bottle cap face central authorities
To aperture, use so that fecaluria Excreta passes through and suture fixed bottle cap, during Excreta is collected, screw on and collect the modeling of Excreta
Material bottle collects Excreta；Less than 4 DEG C are stored in immediately after collecting every time, and bottling is sealed up for safekeeping；
D：Tested cock is fed according to the same mode of step B, tested cock fasting is strong after terminating to raise and corn-like to be measured
Product equal quality without phosphorus diet, and collect the Excreta of tested cock 48h according to the same mode of step C；
E：Content m of phosphorus in the Excreta of the 48h for collecting in determination step C respectively1；The excretion of the 48h collected with step D
Thing collects content m of phosphorus2；Quality measurement is content m of the total phosphorus in the corn sample to be measured of m3；Unit of account quality (1g) is treated
Survey the available phosphorus content m of corn sampleAvailable phosphoruss=m3-(m1-m2)/m；
2) step 1 is taken) the normal com sample of available phosphorus content has been measured, it is put into after crushingSmall sample cup
Carry, excess sample is fitted in specimen cup, scraped off redundance using ruler, and ensure to be fitted into sample surface in specimen cup
It is smooth, the specimen cup for filling sample is placed on rotary bracket, near infrared spectrum scanning is carried out, collection spectral region is 950
Spectral information in～1650nm is interval, every 5nm a spectroscopic data is gathered, and 141 spectroscopic datas are collected altogether, every
Sample repeated measure 2 times, repeats dress sample 2 times；It is the spectral error for reducing scanning, with reference to the light of each surface sweeping after the end of scan
Spectrum information derives the meansigma methodss of spectral information；
3) to step 2) spectral information of all of normal com sample for having measured available phosphorus content that collects adopts
First derivative spectrograply carries out pretreatment with reference to multiplicative scatter correction method, and pretreated spectral information is soft with Applied Chemometrics
Part is associated with the available phosphorus content for having measured of normal com sample, using partial least square method set up near infrared spectrum with
Quantitative model between Semen Maydiss available phosphorus content；
4) take after corn sample to be measured is crushed and be put intoSmall sample cup is carried, and excess sample is loaded into specimen cup
In, redundance is scraped off using ruler, and ensure to be fitted into sample surfacing in specimen cup, the specimen cup of sample will be filled
It is placed on rotary bracket, carries out near infrared spectrum scanning, gathers spectrum letter of the spectral region in 950～1650nm is interval
Breath, every 5nm a spectroscopic data is gathered, and 141 spectroscopic datas are collected altogether, every sample repeated measure 2 times, repeats to fill
Sample 2 times, the spectral information to collecting carries out pretreatment using First derivative spectrograply with reference to multiplicative scatter correction method, after pretreatment
Corn sample near infrared light spectrum information steps for importing 3 to be measured) set up quantitative model, show that corn sample available phosphoruss to be measured contain
Amount.
Above-mentioned near infrared spectrum scanning light source is mercury lamp；Reference source is halogen tungsten lamp；950～1100nm of wave-length coverage is adopted
Silicon detector, 110～1650nm of wave-length coverage adopts diode array detector, wavelength accuracy≤0.3nm, and wavelength stability≤
0.2nm/a；Scanning circumstance is required：2～40 DEG C of temperature, relative humidity 0～85%.
Test example 1
Test method：It is respectively adopted method described in embodiment and gavage determines five kinds of different corn samples in the places of production
Available phosphorus content, compares the accuracy that the inventive method determines available phosphorus content in feed for poultry Semen Maydiss, and measurement result is as follows
Shown in table 1：
Each 6 repeating groups of corn sample setting to be measured in gavage continuous mode, at least 4 chickens of each repeating groups,
Average weight difference is less than 100g between group, and per group of test may proceed to few replication twice, each replication and upper
Secondary measure needs interval to carry out physical efficiency recovery for tested chicken in 10～14 days, free water during physical efficiency recovery, feeding growth egg
Chicken complete feed.
Table 1
| The place of production | Predictive value (%) | Measured value (%) | Difference |
| Shaanxi | 0.15 | 0.14 | 0.01 |
| Northeast | 0.12 | 0.13 | 0.01 |
| Hebei | 0.17 | 0.14 | 0.03 |
| Henan | 0.12 | 0.14 | 0.02 |
| Northeast | 0.13 | 0.11 | 0.02 |
| Averagely | 0.138 | 0.132 | 0.018 |
In table 1, predictive value is available phosphoruss percentage composition in the 1g corn samples to be measured determined using embodiment methods described；
Measured value is available phosphoruss percentage composition in the 1g corn samples to be measured determined using above-mentioned gavage.
From above-mentioned result of the test, the variation amplitude of 5 kinds of corn samples is 0.01～0.03, it is seen that the inventive method pair
Available phosphorus content measurement result in feed for poultry Semen Maydiss is accurate.
Test example 2
Test method：The different corn sample in two kinds of places of production of collection, is designated as sample 1 and sample 2, and prediction group adopts embodiment
Available phosphorus content in methods described determination sample 1 and sample 2, and with the data of the measure as reference, formulating and feeding chicken daily ration,
It is designated as testing daily ration；Matched group looks into the Non-phytate phosphorus value of Semen Maydiss by Database of Feed, and with the data as reference, formulating and feeding
Chicken daily ration, is designated as compareing daily ration.
Test is randomly divided into 4 groups of (prediction group 1,2 and matched groups from 1 age in days AA health broiler chicken 360, male and female half and half
1,2), wherein prediction group 1 feeds the test daily ration prepared by sample 1, and matched group 1 feeds the control daily ration prepared by sample 1, in advance
Survey group 2 feeds the test daily ration prepared by sample 2, and matched group 2 feeds the control daily ration prepared by sample 2, per group of 6 repetitions,
Each repeats 15 chickens.Test chicken cage, free choice feeding drinking-water, routine immunization, temperature, humidity, illumination and health refer in hen house
Reference symbol closes feeding of broiler standard, 42 days experimental periods.The impact of comparative control group and prediction group meat chicken production performance and Slaughter,
As a result as shown in table 2 below and table 3：
Table 2
Table 3
The average daily gain of above-mentioned table 2 and the data display of table 3, prediction group 1 and prediction group 2 is substantially less than the He of matched group 1
Control 2, apparently higher than matched group 1 and matched group 2, feed consumption increases weight ratio apparently higher than matched group 1 and matched group 2 for average daily gain,
Slaughter is without significant difference.The result shows the Semen Maydiss available phosphoruss predicted with the inventive method and uses Non-phytate phosphorus numerical value
The broiler fodder of preparation compares the transformation efficiency that can effectively improve feedstuff, reduces feed waste, reduces aquaculture cost.Thus may be used
See, the near-infrared calibration model that the inventive method builds is applied to the rapid evaluation of Semen Maydiss available phosphoruss, and ensure that feedstuff
The accuracy of cooperation, ensures the performance of meat chicken production performance.
Claims (6)
1. in a kind of feed for poultry Semen Maydiss available phosphorus content rapid assay methods, it is characterised in that including it is following operation step
Suddenly：
1) the normal com sample of known available phosphorus content is taken, near infrared spectrum scanning, collection 950～1650nm of Semen Maydiss areas is carried out
Interior spectral information；
2) spectral information is carried out after pretreatment, the normal com sample of Applied Chemometrics software and known available phosphorus content
The available phosphorus content of product is associated, and using partial least square method determining between near infrared spectrum and Semen Maydiss available phosphorus content is set up
Amount model；
3) taking corn sample to be measured carries out near infrared spectrum scanning, gather 950～1650nm of Semen Maydiss it is interval in spectral information, it is right
Spectral information is carried out after pretreatment, by process after corn sample near infrared light spectrum information steps for importing 2 to be measured) set up determine
Amount model, draws corn sample available phosphorus content to be measured.
2. in feed for poultry Semen Maydiss as claimed in claim 1 available phosphorus content rapid assay methods, it is characterised in that step
It is rapid 1) and step 3) near infrared spectrum scanning light source be mercury lamp；Reference source is halogen tungsten lamp；950～1100nm of wave-length coverage
Using silicon detector, 110～1650nm of wave-length coverage adopts diode array detector, and wavelength accuracy≤0.3nm is Wavelength stabilized
Property≤0.2nm/a；Scanning circumstance is required：2～40 DEG C of temperature, relative humidity 0～85%.
3. in feed for poultry Semen Maydiss as claimed in claim 1 available phosphorus content rapid assay methods, it is characterised in that step
It is rapid 2) and step 3) in pretreatment is carried out near infrared light spectrum information with reference to multiplicative scatter correction method using First derivative spectrograply.
4. in feed for poultry Semen Maydiss as claimed in claim 1 available phosphorus content rapid assay methods, it is characterised in that step
It is rapid 2) in also include that the estimated performance of quantitative model to setting up is evaluated, evaluating mainly has the quantitative model coefficient of determination
R2Cal, correction root-mean-square deviation RMESS, checking root-mean-square deviation RMSEP, checking coefficient of determination R2It is val, number of principal components Ranks, average
Deviation Bias；Screening obtains R2Cal and R2Val is close 1, RMESS and RMSEP is less and closer to quantitative model.
5. in feed for poultry Semen Maydiss as claimed in claim 1 available phosphorus content rapid assay methods, it is characterised in that institute
The available phosphorus content stated in normal com sample is detected that concrete operation step is using following methods：
A：From it is more than body weight 1.8kg, body weight is close, feeding is normal, it is strong raise after be as good as paradoxical reaction, the health sea without odd habit it is blue brown
Some of cock, single cage is raised, and raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and daily light application time is 16 hours, freely
Drinking-water, fasting sandstone；
B：Tested cock feeding laying hens in growth period complete feed more than three days, and after final stage feeding corn sample to be measured, enter
Row fasting is emptied 48 hours, by drinking-water per chicken daily iron supplement glucose 50g during fasting；
C：Fasting after terminating strong quality of raising be m, unit is g, corn sample to be measured, the continuous Excreta for collecting 48 hours；
D：Tested cock is fed according to the same mode of step B, tested cock fasting is raised by force same with corn sample to be measured after terminating
Etc. quality without phosphorus diet, and the Excreta of tested cock 48h is collected according to the same mode of step C；
E：Content m of phosphorus in the Excreta of the 48h for collecting in determination step C respectively1；The Excreta of the 48h collected with step D is converged
Content m of total phosphorus2；Quality measurement is content m of the total phosphorus in the corn sample to be measured of m3；Unit of account quality 1g, Semen Maydiss to be measured
The available phosphorus content m of sampleAvailable phosphoruss=m3-(m1-m2)/m。
6. the rapid assay methods of available phosphorus content exist in a kind of feed for poultry Semen Maydiss as described in any one of Claims 1 to 5
Application in control poultry Semen Maydiss diet feed in terms of phosphorus content.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107273656A (en) * | 2017-05-11 | 2017-10-20 | 广州讯动网络科技有限公司 | A kind of quantitative model appraisal procedure and system based on Molecular Spectral Analysis |
| CN109886482A (en) * | 2019-02-01 | 2019-06-14 | 中国农业大学 | The near-infrared method for quick predicting of pig digestible energy and metabolic energy in a kind of wheat bran |
| CN109886481A (en) * | 2019-02-01 | 2019-06-14 | 中国农业大学 | The near-infrared method for quick predicting of pig digestible energy and metabolic energy in corn protein powder |
| CN113167789A (en) * | 2018-09-14 | 2021-07-23 | 国家农业食品及环境研究院 | Serum color as a biomarker for digestive efficiency of poultry |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102901797A (en) * | 2012-09-20 | 2013-01-30 | 中国农业科学院北京畜牧兽医研究所 | Bionic evaluation method of available phosphorus in pig feed |
-
2016
- 2016-10-17 CN CN201610903622.4A patent/CN106645019A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102901797A (en) * | 2012-09-20 | 2013-01-30 | 中国农业科学院北京畜牧兽医研究所 | Bionic evaluation method of available phosphorus in pig feed |
Non-Patent Citations (5)
| Title |
|---|
| 中华人民共和国国家质量监督检验验疫总局中国国家标准化管理委员会: "畜禽饲料有效性与安全性评价 强饲法测定鸡饲料表观代谢能技术规程", 《GB/T26437-2010畜禽饲料有效性与安全性评价 强饲法测定鸡饲料表观代谢能技术规程》 * |
| 侯水生等: "鸡盲肠对饲料磷的消化作用", 《畜牧兽医学报》 * |
| 孔源等: "近红外技术快速测定肉鸡粪便主要肥料主成分含量的研究", 《农业工程学报》 * |
| 廖瑞波: "肉鸡的玉米标准回肠可消化氨基酸测定及近红外定标模型建立", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 陈兰珍等: "《蜂蜜近红外光谱检测技术》", 31 January 2012, 中国轻工业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107273656A (en) * | 2017-05-11 | 2017-10-20 | 广州讯动网络科技有限公司 | A kind of quantitative model appraisal procedure and system based on Molecular Spectral Analysis |
| CN113167789A (en) * | 2018-09-14 | 2021-07-23 | 国家农业食品及环境研究院 | Serum color as a biomarker for digestive efficiency of poultry |
| CN109886482A (en) * | 2019-02-01 | 2019-06-14 | 中国农业大学 | The near-infrared method for quick predicting of pig digestible energy and metabolic energy in a kind of wheat bran |
| CN109886481A (en) * | 2019-02-01 | 2019-06-14 | 中国农业大学 | The near-infrared method for quick predicting of pig digestible energy and metabolic energy in corn protein powder |
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