CN106442398A - Rapid measuring method for content of available phosphorus in wheat for feeding poultry and application - Google Patents
Rapid measuring method for content of available phosphorus in wheat for feeding poultry and application Download PDFInfo
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- CN106442398A CN106442398A CN201610903630.9A CN201610903630A CN106442398A CN 106442398 A CN106442398 A CN 106442398A CN 201610903630 A CN201610903630 A CN 201610903630A CN 106442398 A CN106442398 A CN 106442398A
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 104
- 239000011574 phosphorus Substances 0.000 title claims abstract description 104
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 101
- 241000209140 Triticum Species 0.000 title claims abstract description 88
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 88
- 244000144977 poultry Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000002329 infrared spectrum Methods 0.000 claims abstract description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 210000003608 fece Anatomy 0.000 claims description 20
- 230000003595 spectral effect Effects 0.000 claims description 19
- 230000002354 daily effect Effects 0.000 claims description 15
- 238000003556 assay Methods 0.000 claims description 14
- 238000005259 measurement Methods 0.000 claims description 14
- 235000005911 diet Nutrition 0.000 claims description 12
- 238000001228 spectrum Methods 0.000 claims description 10
- 230000037213 diet Effects 0.000 claims description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 5
- 230000000378 dietary effect Effects 0.000 claims description 4
- 239000003651 drinking water Substances 0.000 claims description 4
- 235000020188 drinking water Nutrition 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 125000005843 halogen group Chemical group 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 3
- 229910052721 tungsten Inorganic materials 0.000 claims description 3
- 239000010937 tungsten Substances 0.000 claims description 3
- 238000012937 correction Methods 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 241000233855 Orchidaceae Species 0.000 claims 1
- 238000012545 processing Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 20
- 235000013330 chicken meat Nutrition 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000003304 gavage Methods 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000004611 spectroscopical analysis Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 3
- 235000002949 phytic acid Nutrition 0.000 description 3
- 206010059410 Faecaluria Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 230000008979 phosphorus utilization Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
Abstract
The invention relates to the technical field of feed detection, in particular to a rapid measuring method for the content of available phosphorus in wheat for feeding poultry and application. According to the method, the near infrared spectrum technology is utilized, firstly, a quantitative model between a near infrared spectrum with the feature wavebands of 950-1,650 nm and the content of available phosphorus of the wheat is established, then the feature waveband near infrared spectrum of a to-be-measured sample is scanned and substituted into the quantitative model, and the content of available phosphorus of the to-be-measured sample is obtained. By means of the method, the content of available phosphorus in the wheat for feeding poultry can be rapidly and accurately measured, the content of available phosphorus serves as indexes for preparation of the feed for feeding poultry, the accuracy of feed matching can be guaranteed, the conversion efficiency of the feed is effectively improved, feed waste is reduced, exertion of the production performance of broilers is guaranteed, and the breeding cost is reduced.
Description
Technical field
The present invention relates to feed detection technique field, be specifically related to the fast of available phosphorus content in a kind of feed for poultry wheat
Speed assay method and application.
Background technology
Phosphorus is one of required mineral element of poultry, and it is low that Dietary phosphorus wretched insufficiency shows as production performance, and childhood is moved
Thing suffers from rickets, and adults suffers from malacosis (or claiming osteoporosis).The requirement to phosphorus for the poultry generally use total phosphorus (TP),
Non-phytate phosphorus (NPP) and available phosphorus (AP) statement (NRC, 1994；Fowl raising standard, 2004).Previously think, in vegetable raw material
The 1/3 of total phosphorus is NPP, and other 2/3 is the phytate phosphorus that can not be utilized by nonruminant, has so being equal to NPP simply
Effect phosphorus (NRC, 1994).But the research of existing some scholars proves, animal can utilize phytate phosphorus to a certain extent, and its
Utilization rate has larger difference because raw material type is different, and animal is to the difference of phytate phosphorus utilization rate in vegetality feedstuff and examination
Test design, research method, feedstuff and trophic component, process, analysis method, animal age in days and kind etc. because have
Close, and the Non-phytate phosphorus that laboratory records can not be utilized completely by animal.Thus total phosphorus and Non-phytate phosphorus all can not be really anti-
Reflecting the titer of phosphorus in feedstuff, with the accumulation of research work, available phosphorus necessarily replaces Non-phytate phosphorus as evaluation phosphate use
The index of rate.
In consideration of it, research and develop a kind of can apply in poultry Feed Manufacturing simplicity, quick, accurately evaluate wheat
The method of middle available phosphorus, has great importance for realizing that preparation poultry is precisely raised efficiently to utilize with feedstuff.
Content of the invention
In order to overcome the defect of prior art, it is an object of the invention to provide available phosphorus in a kind of feed for poultry wheat and contain
The rapid assay methods of amount, it is achieved the content of available phosphorus in quick, Accurate Determining feed for poultry wheat, improves feed formulation
Accuracy, reduces phosphorus and pollutes, save feed cost.
Meanwhile, the present invention also resides in the rapid assay methods providing available phosphorus content in a kind of feed for poultry wheat in control
Application in terms of poultry Wheat Based Diet Dietary phosphorus content processed.
In order to realize object above, the technical solution adopted in the present invention is：
The rapid assay methods of available phosphorus content in a kind of feed for poultry wheat, including following operating procedure：
1) take the standard wheat samples of known available phosphorus content, carry out near infrared spectrum scanning, gather wheat 950～
Spectral information in 1650nm is interval；
2), after pre-processing spectral information, Applied Chemometrics software is little with the standard of known available phosphorus content
The available phosphorus content of wheat sample is associated, and uses PLS to set up between near infrared spectrum and wheat available phosphorus content
Quantitative model；
3) take wheat samples to be measured and carry out near infrared spectrum scanning, gather the spectrum letter in wheat 950～1650nm interval
Breath, after spectral information is pre-processed, will process after wheat samples near infrared light spectrum information steps for importing 2 to be measured) build
Vertical quantitative model, draws wheat samples available phosphorus content to be measured.
Further, step 1) and step 3) in the light source of near infrared spectrum scanning be mercury lamp；Reference source is halogen tungsten lamp；
Wave-length coverage 950～1100nm uses silicon detector, and wave-length coverage 110～1650nm uses PDAD, wavelength
Precision≤0.3nm, wavelength stability≤0.2nm/a；Scanning circumstance requires：Temperature 2～40 DEG C, relative humidity 0～85%.
Further, affect spectra collection to prevent sample from going mouldy, the sample collecting is placed in 4 DEG C of refrigerators
Preserve, before scanning sample, within 24 hours in advance, sample is placed on equilibrium temperature in normal temperature environment, after scanning start, preheats 1h.
Further, step 2) and step 3) in use First derivative spectrograply near infrared light spectrum information is pre-processed.
Further, step 2) in also include evaluating the estimated performance of quantitative model set up, evaluating master
Quantitative model coefficient of determination R to be had2Cal, correction root-mean-square deviation RMESS, checking root-mean-square deviation RMSEP, the checking coefficient of determination
R2Val, number of principal components Ranks, average deviation Bias；Screening obtains R2Cal and R2Val less close to the 1st, RMESS and RMSEP and
Closer to quantitative model.
Above-mentioned to step 2) concrete grammar evaluated of the estimated performance of quantitative model set up stays one alternately for using
Checking, i.e. rejects a sample from the standard wheat samples of known available phosphorus content, sets up mathematics with other samples remaining
Model, and by the concentration of component to be measured in the disallowable sample of model prediction set up, the prediction effect of model is evaluated.
Wherein R2The capability of fitting of cal reaction model, R2Cal is closer to 1, and the fitness of model is better, and its computing formula is
Wherein：ym-sample practical measurement value mean value；yiI-th sample practical measurement value；Use the near of foundation
The predicted value of i-th sample of infrared spectrum quantitative model；
RMSEE computing formula is：
Wherein,Sample practical measurement value mean value.
RMSEP represents the close degree between near-infrared predicted value and practical measurement value, and the lower accuracy of numerical value is higher.Its
Computing formula is：
Average deviation is the system deviation of practical measurement value and near-infrared predicted value, and its computing formula is：
Wherein：di-i-th sample practical measurement value and the difference of predicted value.
Further, the available phosphorus content in described standard wheat samples uses gavage to record, as standard wheat sample
The known available phosphorus content of product, concrete operation step is：
A：Select more than body weight 1.8kg, body weight is close, it is normal to search for food, raise by force after reaction without exception, healthy extra large without odd habit
Some of blue brown cock, single cage raising, raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and every day, light application time was 16 hours,
Freely drink water, fasting sandstone；
B：Tested cock feeds laying hens in growth period complete feed more than three days, and final stage feeds wheat samples to be measured
After, carry out fasting and empty 48 hours, by the every chicken daily iron supplement glucose 50g of drinking-water during fasting；
C：Quality is raised by force in fasting after terminating be m (unit is g), the wheat samples to be measured of 40～50g, collects 48 hours continuously
Excreta；Can use and grow stitching 60ml plastic bottle closure at accent periphery rushing down of tested cock, a circle digs in bottle cap face central authorities
Hole and 4 pairs of apertures of symmetry, in order to fecaluria excreta passes through and sews up fixing bottle cap use, during collecting excreta, receipts of screwing on
The plastic bottle of collection excreta collects excreta；Or use collection excrement dish to collect excreta；During excreta collection, every day collects
Several times, being saved in less than 4 DEG C immediately after collecting every time, or directly drying at 60～65 DEG C to constant weight, bottling is sealed up for safekeeping；
D：Feeding tested cock according to the same mode of step B, tested cock fasting is raised by force after terminating and wheat sample to be measured
Product equal quality without phosphorus diet, and collect the excreta of tested cock 48h according to the same mode of step C；
E：The content m of phosphorus in the excreta of the 48h collecting in determination step C respectively1；Excretion with the 48h that step D is collected
Thing collects the content m of phosphorus2；Quality measurement is the content m of the total phosphorus in the wheat samples to be measured of m3；Unit of account quality (1g) is treated
Survey the available phosphorus content m of wheat samplesAvailable phosphorus=m3-(m1-m2)/m.
Total phosphorus content in above-mentioned excreta and wheat samples to be measured uses Yang Sheng《Forage analysis and determination of feeds quality
Technology》Disclosed in method detect.In above-mentioned feed for poultry wheat, the rapid assay methods of available phosphorus content is in control
Application in terms of poultry Wheat Based Diet Dietary phosphorus content, is specially to use said determination method detection feed for poultry wheat
In available phosphorus content as index, according to the demand for phosphorus content in poultry diet, determine the consumption of wheat, can effectively carry
The transformation efficiency of phosphorus in high daily ration, reduces daily ration waste and phosphorus pollutes, and reduces feeding cost.
The rapid assay methods of available phosphorus content in feed for poultry wheat of the present invention, uses near-infrared spectrum technique, adopts
Spectral information in collection wheat 950～1650nm is interval, measures the phosphorus content in wheat fast and accurately, feeds house as wheat
Available phosphorus index during fowl, has advantage simple to operate, that accuracy is high, can provide for the phosphorus content in feed formulation
Data-guiding accurately, improves the utilization rate of phosphorus in poultry Wheat Based Diet, reduces the pollution of phosphorus, saves feed cost.
Further, during near infrared spectrum detection, the resolution ratio of spectral scan, detection mode, spectral scan model
Enclose, scanning circumstance etc. all can affect the accuracy of near infrared spectrum detection, and for different mensuration samples, its influence
Being also uncertain, the especially preprocess method of spectrum has a variety of, then feed house in order to improve in detection in the present invention
The accuracy of available phosphorus content in fowl wheat, the Parameter Conditions of creative selection spectral scan, and combine First derivative spectrograply
Near infrared spectrum is pre-processed.
Further, during near infrared spectrum scanning, the accuracy of the quantitative model of foundation is determined to a great extent
Determine the accuracy of final measurement result, in order to improve reliability, stability and the dynamic adaptable of quantitative model in the present invention,
Use the available phosphorus content in gavage bioassay standard wheat samples.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in detail.
Embodiment
The rapid assay methods of available phosphorus content in a kind of feed for poultry wheat, including following operating procedure：
1) take 10～20 parts of different standard wheat samples of available phosphorus content, be respectively adopted gavage bioassay standard wheat sample
Phosphorus content in product, as the available phosphorus standard content of standard wheat samples, concrete operation step is：
A：Select more than body weight 1.8kg, body weight is close, it is normal to search for food, raise by force after reaction without exception, healthy extra large without odd habit
Some of blue brown cock, single cage raising, raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and every day, light application time was 16 hours,
Freely drink water, fasting sandstone；
B：Tested cock feeds laying hens in growth period complete feed more than three days, and final stage feeds wheat samples to be measured
After, carry out fasting and empty 48 hours, by the every chicken daily iron supplement glucose 50g of drinking-water during fasting；
C：Quality is raised by force in fasting after terminating be m (unit is g), the wheat samples to be measured of 40～50g, collects 48 hours continuously
Excreta；Use and grow at accent periphery stitching 60ml plastic bottle closure rushing down of tested cock, bottle cap face central authorities dig a circular hole and
4 pairs of symmetrical apertures, in order to fecaluria excreta passes through and sews up fixing bottle cap use, during collecting excreta, collection of screwing on is arranged
The plastic bottle letting out thing collects excreta；Being saved in less than 4 DEG C immediately after collecting every time, bottling is sealed up for safekeeping；
D：Feeding tested cock according to the same mode of step B, tested cock fasting is raised by force after terminating and wheat sample to be measured
Product equal quality without phosphorus diet, and collect the excreta of tested cock 48h according to the same mode of step C；
E：The content m of phosphorus in the excreta of the 48h collecting in determination step C respectively1；Excretion with the 48h that step D is collected
Thing collects the content m of phosphorus2；Quality measurement is the content m of the total phosphorus in the wheat samples to be measured of m3；Unit of account quality (1g) is treated
Survey the available phosphorus content m of wheat samplesAvailable phosphorus=m3-(m1-m2)/m；
2) step 1 is taken) record the standard wheat samples of available phosphorus content, put into after pulverizingSmall sample cup
Carry, excess sample is loaded in specimen cup, use ruler to scrape off redundance, and ensure to load sample surface in specimen cup
Smooth, it is placed on the specimen cup filling sample on rotary bracket, carries out near infrared spectrum scanning, gather spectral region 950
Spectral information in～1650nm is interval, gathers a spectroscopic data every 5nm, altogether collects 141 spectroscopic datas, every
Sample duplicate measurements 2 times, repeats dress sample 2 times；After the end of scan, for reducing the spectral error of scanning, in conjunction with the light of each surface sweeping
Spectrum information derives the mean value of spectral information；
3) to step 2) spectral information of all of standard wheat samples having recorded available phosphorus content that collects uses
First derivative spectrograply pre-processes, by pretreated spectral information Applied Chemometrics software and standard wheat samples
The available phosphorus content having recorded is associated, and uses PLS to set up between near infrared spectrum and wheat available phosphorus content
Quantitative model；
4) take after wheat samples to be measured is pulverized and put intoSmall sample cup carries, and excess sample is loaded specimen cup
In, use ruler to scrape off redundance, and ensure to load sample surfacing in specimen cup, the specimen cup of sample will be filled
It is placed on rotary bracket, carries out near infrared spectrum scanning, gather spectrum letter in 950～1650nm is interval for the spectral region
Breath, gathers a spectroscopic data every 5nm, altogether collects 141 spectroscopic datas, every sample duplicate measurements 2 times, repeats dress
Sample 2 times, uses First derivative spectrograply to pre-process the spectral information that collects, by closely red for pretreated wheat samples to be measured
External spectrum information steps for importing 3) quantitative model set up, draw wheat samples available phosphorus content to be measured.
Above-mentioned near infrared spectrum scanning light source is mercury lamp；Reference source is halogen tungsten lamp；Wave-length coverage 950～1100nm uses
Silicon detector, wave-length coverage 110～1650nm uses PDAD, wavelength accuracy≤0.3nm, wavelength stability≤
0.2nm/a；Scanning circumstance requires：Temperature 2～40 DEG C, relative humidity 0～85%.
Test example 1
Test method：The method described in embodiment that is respectively adopted measures five kinds of different wheat samples in the place of production with gavage
Available phosphorus content, compares the inventive method and measures the accuracy of available phosphorus content in feed for poultry wheat, and measurement result is as follows
Shown in table 1：
In gavage process of the test, each wheat samples to be measured arranges 6 repeating groups, at least 4 chickens of each repeating groups,
Between group, average weight difference is less than 100g, often organizes test and may proceed to few replication twice, each replication and upper
Secondary mensuration needs to be spaced 10～14 days and carries out physical efficiency recovery for tested chicken, freely drinks water, feed growth egg during physical efficiency recovery
Chicken complete feed.
Table 1
| The place of production | Predicted value (%) | Measured value (%) | Difference |
| Shandong | 0.249 | 0.228 | 0.021 |
| Henan | 0.235 | 0.223 | 0.012 |
| Jiangsu | 0.221 | 0.234 | 0.013 |
| Hebei | 0.214 | 0.268 | 0.054 |
| Anhui | 0.259 | 0.249 | 0.01 |
| Averagely | 0.236 | 0.240 | 0.022 |
In table 1, it was predicted that be worth available phosphorus percentage composition in the 1g wheat samples to be measured that method described in employing embodiment measures；
Available phosphorus percentage composition in the 1g wheat samples to be measured that measured value measures for using above-mentioned gavage.
From above-mentioned result of the test, the variation amplitude of 5 kinds of wheat samples is 0.01～0.02, it is seen that the inventive method pair
Available phosphorus content measurement result in feed for poultry wheat is accurate.
Test example 2
Test method：Gather the different wheat samples in two kinds of places of production, be designated as sample 1 and sample 2, it was predicted that group uses embodiment
Described method measures available phosphorus content in sample 1 and sample 2, and with the data of this mensuration as reference, formulating and feeding chicken daily ration,
It is designated as testing daily ration；Control group looks into the Non-phytate phosphorus value of wheat by Database of Feed, and with this data as reference, formulating and feeding
Chicken daily ration, is designated as compareing daily ration.
The healthy Broiler chicks 360 of 1 age in days AA, male and female half and half are selected in test, are randomly divided into 4 groups of (prediction group 1,2 and control groups
1,2), wherein prediction group 1 feeds the test daily ration prepared by sample 1, and control group 1 feeds the comparison daily ration prepared by sample 1, in advance
Survey group 2 feeds the test daily ration prepared by sample 2, and control group 2 feeds the comparison daily ration prepared by sample 2, often organizes 6 repetitions,
Each repeats 15 chickens.Test chicken is raised in cages, and free choice feeding is drunk water, and routine immunization, in hen house, temperature, humidity, illumination and health refer to
Reference symbol closes feeding of broiler standard, 42 days experimental periods.The relatively impact of control group and prediction group meat chicken production performance and Slaughter,
Result is as shown in table 2 below and table 3：
Table 2
Table 3
Above-mentioned table 2 and table 3 data show, it was predicted that the average daily ingestion amount of group 1 and prediction group 2 is substantially less than control group 1 He
Comparison 2, average daily gain apparently higher than control group 1 and control group 2, feed consumption weightening finish ratio apparently higher than control group 1 and control group 2,
Slaughter is all without significant difference.This result shows with the wheat available phosphorus of the inventive method prediction and use Non-phytate phosphorus numerical value
The broiler fodder of preparation compares the transformation efficiency that can effectively improve feed, reduces feed waste, reduces aquaculture cost.Thus may be used
Seeing, the near-infrared calibration model that the inventive method builds is applicable to the rapid evaluation of wheat available phosphorus, and ensure that feed
The accuracy coordinating, ensures the performance of meat chicken production performance.
Claims (6)
1. the rapid assay methods of available phosphorus content in a feed for poultry wheat, it is characterised in that include following operation step
Suddenly：
1) take the standard wheat samples of known available phosphorus content, carry out near infrared spectrum scanning, gather wheat 950～1650nm district
Interior spectral information；
2) after spectral information being pre-processed, the standard wheat sample of Applied Chemometrics software and known available phosphorus content
The available phosphorus content of product is associated, and uses PLS to set up determining between near infrared spectrum and wheat available phosphorus content
Amount model；
3) take wheat samples to be measured and carry out near infrared spectrum scanning, gather the spectral information in wheat 950～1650nm interval, right
After spectral information pre-processes, wheat samples near infrared light spectrum information steps for importing 2 to be measured after processing) determining of setting up
Amount model, draws wheat samples available phosphorus content to be measured.
2. the rapid assay methods of available phosphorus content in feed for poultry wheat as claimed in claim 1, it is characterised in that step
Rapid 1) and step 3) in the light source of near infrared spectrum scanning be mercury lamp；Reference source is halogen tungsten lamp；Wave-length coverage 950～1100nm
Using silicon detector, wave-length coverage 110～1650nm uses PDAD, and wavelength accuracy≤0.3nm is Wavelength stabilized
Property≤0.2nm/a；Scanning circumstance requires：Temperature 2～40 DEG C, relative humidity 0～85%.
3. the rapid assay methods of available phosphorus content in feed for poultry wheat as claimed in claim 1, it is characterised in that step
Rapid 2) and step 3) in use First derivative spectrograply near infrared light spectrum information is pre-processed.
4. the rapid assay methods of available phosphorus content in feed for poultry wheat as claimed in claim 1, it is characterised in that step
Rapid 2) also including in evaluating the estimated performance of the quantitative model set up, evaluating mainly has the quantitative model coefficient of determination
R2Cal, correction root-mean-square deviation RMESS, checking root-mean-square deviation RMSEP, checking coefficient of determination R2Val, number of principal components Ranks, average
Deviation Bias；Screening obtains R2Cal and R2Val less close to the 1st, RMESS and RMSEP and closer to quantitative model.
5. the rapid assay methods of available phosphorus content in feed for poultry wheat as claimed in claim 1, it is characterised in that institute
Stating the available phosphorus content in standard wheat samples uses following methods to detect, and concrete operation step is：
A：Select more than body weight 1.8kg, body weight is close, it is normal to search for food, raise by force after reaction without exception, brown without the healthy sea orchid of odd habit
Some of cock, single cage raising, raising temperature is 15～27 DEG C, intensity of illumination 20Lx, and every day, light application time was 16 hours, freely
Drinking-water, fasting sandstone；
B：After tested cock feeds laying hens in growth period complete feed more than three days, and final stage feeds wheat samples to be measured, enter
Row fasting empties 48 hours, by the every chicken daily iron supplement glucose 50g of drinking-water during fasting；
C：It is m that fasting raises by force quality after terminating, and unit is g, wheat samples to be measured, collect continuously the excreta of 48 hours；
D：Feeding tested cock according to the same mode of step B, tested cock fasting is raised by force same with wheat samples to be measured after terminating
Etc. quality without phosphorus diet, and collect the excreta of tested cock 48h according to the same mode of step C；
E：The content m of phosphorus in the excreta of the 48h collecting in determination step C respectively1；Excreta remittance with the 48h that step D is collected
The content m of total phosphorus2；Quality measurement is the content m of the total phosphorus in the wheat samples to be measured of m3；Unit of account quality 1g, wheat to be measured
The available phosphorus content m of sampleAvailable phosphorus=m3-(m1-m2)/m.
6. in a feed for poultry wheat as described in any one of Claims 1 to 5, the rapid assay methods of available phosphorus content exists
Application in terms of control poultry Wheat Based Diet Dietary phosphorus content.
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| US20150168364A1 (en) * | 2013-12-17 | 2015-06-18 | Alltech, Inc. | Systems and methods for adjusting animal feed |
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