CN106644626B - A kind of permanent magnetism enclosed experiment device for crystallization of protein - Google Patents
A kind of permanent magnetism enclosed experiment device for crystallization of protein Download PDFInfo
- Publication number
- CN106644626B CN106644626B CN201610898306.2A CN201610898306A CN106644626B CN 106644626 B CN106644626 B CN 106644626B CN 201610898306 A CN201610898306 A CN 201610898306A CN 106644626 B CN106644626 B CN 106644626B
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- aluminium sheet
- crystallization
- protein
- diamagnetic
- permanent
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F7/00—Magnets
- H01F7/02—Permanent magnets [PM]
- H01F7/0231—Magnetic circuits with PM for power or force generation
- H01F7/0252—PM holding devices
- H01F7/0263—Closures, bags, bands, engagement devices with male and female parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/05—Investigating materials by wave or particle radiation by diffraction, scatter or reflection
- G01N2223/056—Investigating materials by wave or particle radiation by diffraction, scatter or reflection diffraction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/10—Different kinds of radiation or particles
- G01N2223/101—Different kinds of radiation or particles electromagnetic radiation
- G01N2223/1016—X-ray
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/60—Specific applications or type of materials
- G01N2223/612—Specific applications or type of materials biological material
Abstract
The present invention provides a kind of permanent magnetism enclosed experiment devices for crystallization of protein, and two block permanent magnets are placed in parallel, and the polarity of two block permanent magnet corresponding positions is opposite；U-shaped aluminium sheet between two block permanent magnets, aluminium sheet and permanent magnet enclose five bandings close, the crystallization plates rest area of openings at one side；Several paramagnetic steel plates and diamagnetic aluminium sheet enclose an enclosed construction, permanent magnet and U-shaped aluminium sheet are wrapped in, in the enclosed construction, the two sides of the U-shaped aluminium sheet opening of face are diamagnetic aluminium sheet.Magnetic field strength of the present invention is big, and volume compact, manufacturing cost is low, can significantly improve crystallization of protein quality.
Description
Technical field
The present invention relates to Bioexperiment instrument fields, and in particular to a kind of permanent magnetism closing for bioprotein crystallization is real
Experiment device.
Background technique
Protein is the main undertaker of vital movement, has extremely changeable structure and function, therefore it is living to life
Dynamic importance is self-evident.By the analysis of parsing and function to protein structure, we can be living to life
It is dynamic that there is deeper understanding and research.
Currently, the parsing of protein structure is mainly by XRD (X-ray diffraction) come what is completed, and in PDB
The protein structure that 90% is had more than in (Protein Data Bank) database is parsed by XRD.XRD is in albumen at present
There is absolute dominant position during matter structural analysis, but needed using XRD parsing protein structure using protein crystal,
Therefore crystallization of protein becomes in order to which how the committed step of XRD protein structure parsing obtains high-quality on the current technology
The protein crystal of amount is still the bottleneck for restricting the development of this technology.
Document " Effects of a magnetic field on the nucleation and growth of
Protein crystals " etc. report carried out in magnetic field crystallization of protein can significantly generate that quantity is less but volume more
Big protein crystal.Therefore the crystal quality that crystallization of protein is improved by the particular surroundings in magnetic field is to improve XRD albumen
One of the effective way of matter diffraction quality.
According to the mode that is differently formed of magnetic force, magnetic field can be divided into electromagnetic field and permanent magnetic field.Electromagnetic field can be divided into routine again
Conductor magnetic field and cryogenic magnetic field, electromagnetic field can generate the magnetic field compared with high magnetic force under the action of electric current, from several teslas to several
Equal magnetic field strength does not need special in use although electromagnetic field has higher magnetic field strength for ten teslas
Place, higher manpower and material resources go to safeguard, put into it is larger, it is inconvenient to use.On the other hand for permanent magnetic field, magnetic field is strong
Degree reaches as high as a little several teslas at present, although it is small much to compare its intensity with electromagnetic field, permanent magnetic field device
Small volume, once component devices many and diverse maintenance that there is no need to carry out the input of energy again and as electromagnetic field.Simultaneously
During the protein crystallization experiments of underway low field strength, permanent magnetic field also has important experiment and more practical value.
Summary of the invention
For overcome the deficiencies in the prior art, it is real with the closing of crystallization of protein permanent magnetism to provide a kind of practical test by the present invention
Experiment device, magnetic field strength of the present invention is big, and volume compact, manufacturing cost is low, can significantly improve crystallization of protein quality.
The technical solution adopted by the present invention to solve the technical problems is: a kind of permanent magnetism envelope for bioprotein crystallization
Close experimental provision, including permanent magnet, U-shaped aluminium sheet, diamagnetic aluminium sheet and paramagnetic steel plate；Permanent magnet described in two pieces is placed in parallel, and
The polarity of two block permanent magnet corresponding positions is opposite；Between two block permanent magnets, aluminium sheet and permanent magnet enclose the U-shaped aluminium sheet
It is closed at five bandings, the crystallization plates rest area of openings at one side；Several paramagnetic steel plates and diamagnetic aluminium sheet enclose a closing knot
Structure, permanent magnet and U-shaped aluminium sheet are wrapped in, in the enclosed construction, the two sides of the U-shaped aluminium sheet opening of face are diamagnetic aluminium
Plate.
The permanent magnet uses neodymium iron boron strong permanent magnetic iron.
Respectively there are two block permanent magnets in the U-shaped aluminium sheet two sides, and two block permanent magnets of the same side, which are generally aligned in the same plane to be adjacent to, to be put
It sets, and the polarity of the adjacent side of two block permanent magnets is opposite.
There are spaces between enclosed construction in the plane locating for itself for the permanent magnet.
In the enclosed construction, the position of the diamagnetic U-shaped aluminium sheet opening of aluminium sheet face is provided with crystallization plates entrance, and passes through door
Lid closing.
The paramagnetic steel plate is fastenedly connected by paramagnetic screw rod, and the diamagnetic aluminium sheet is installed by paramagnetic screw rod
On paramagnetic steel plate.
The permanent magnet is mounted on paramagnetic steel plate by magnetic cylindrical pins.
The beneficial effects of the present invention are: magnet is thin firstly, permanent magnet of the present invention is neodymium iron boron strong permanent magnetic iron
Rectangle can reduce monolithic large area pole center position field strength in this way and weaken problem up and down respectively close to two pieces.Using N-S magnetic
Extremely opposite and magnet Parallel Design, while the whole diamagnetic design of closure paramagnetic steel plate and front and back of outside of deivce face surrounding, greatly
The outer dissipation for reducing the magnetic line of force greatly, had not only greatly strengthened the magnetic field strength inside device in this way, but also made basic dimension inside device
Hold permanent steady magnetic field strength.Secondly, accompanying U-shaped diamagnetic aluminium sheet between magnet up and down, the magnetic line of force between such magnet is most of all
It is concentrated to the crystallization plates rest area of U-shaped indentation, there, when design utilizes ANSYS finite element modelling and analysis, device internal crystallization
Plate rest area establishes the constant magnetic field for meeting crystallization plates size, after device completes, to the magnetic inside device
Field intensity carries out actual measurement, as a result and simulation in advance calculate the result is that almost the same, be advantageous for later experiments in this way
The requirement of condition homogeneity increases accuracy and the experiment effect of experiment.Finally, apparatus of the present invention are small in size, it, can convenient for moving
It is placed in water circulator bath, occupancy lab space is small, easy to use.
Detailed description of the invention
Fig. 1 is experimental provision surface structure schematic diagram of the present invention.
Fig. 2 is experimental provision schematic diagram of internal structure of the present invention.
Fig. 3 is that experimental provision of the present invention opens the internal structure top view after top paramagnetic steel plate.
Fig. 4 is interior after experimental provision of the present invention opening top paramagnetic steel plate and withdrawing device internal upper part permanent magnet
Portion's structure top view.
Internal core (permanent magnet and U-shaped aluminium sheet) structural schematic diagram of Fig. 5 experimental provision of the present invention.
The device outer closures system paramagnetic steel plate splicing structural schematic diagram of Fig. 6 experimental provision of the present invention.
In figure, 1.1, diamagnetic cylindrical pins；1.2, upper paramagnetic steel plate；1.3, big paramagnetic screw rod；1.4, small paramagnetic screw
Bar；1.5, entrance door closure；1.6, preceding diamagnetic aluminium sheet；1.7, left paramagnetic steel plate；2.1, upper paramagnetic plate；2.2, right paramagnetic steel plate；
2.3, U-shaped aluminium sheet；2.4, diamagnetic air space；2.5, lower paramagnetic steel plate；2.6, lower strong permanent magnetic iron A；2.7, crystallization plates rest area；
2.8, upper strong permanent magnetic iron A；3.1, rear diamagnetic aluminium sheet；3.2, device portal；5.1, upper strong permanent magnetic iron B；5.2, lower strong permanent magnetic iron B；
6.1, U-shaped aluminium sheet locating slot；6.2, strong permanent magnetic iron locating slot.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples, and the present invention includes but are not limited to following implementations
Example.
The present invention provides a kind of permanent magnetism enclosed experiment device for bioprotein crystallization, including device closed test system
System and device outer closures system.
The device Internal Experiment system includes: U-shaped thickness diamagnetic duralumin plate (the U-shaped aluminium of strong permanent magnetic iron, supporting function
Plate), crystallization plates rest area and diamagnetic air space.
The device outer closures system include: paramagnetic steel plate, diamagnetic aluminium sheet, paramagnetic screw rod, diamagnetic cylindrical pins,
Device portal and entrance door closure.
As the preferred embodiment of above-mentioned technology, the device Internal Experiment system is wrapped completely by device outer closures system
It wraps up in and is enclosed in inside.
As the preferred embodiment of above-mentioned technology, the strong permanent magnetic iron is the generating source of medium-high magnetic field of the present invention, in device
Internal strong permanent magnetic iron generates almost permanent steady strong magnetic field action on crystallization plates when experiment, each two blocks of magnet up and down in device,
It is parallel opposite.
As the preferred embodiment of above-mentioned technology, the U-shaped aluminium sheet by not homopolarity to face strong magnet support separate
It opens, prevents it from attracting each other and be attached onto.
As the preferred embodiment of above-mentioned technology, the crystallization plates rest area is located among strong permanent magnetic iron opposing upper and lower, U
Placement inside the U-shaped of type aluminium sheet, in experiment for crystallization plates.
As the preferred embodiment of above-mentioned technology, the diamagnetic air space is located at upper and lower magnet two sides, primarily serves anti-
Magnetic is enclosed in the magnetic line of force mainly in the paramagnetic steel plate in device outer closures system.
As the preferred embodiment of above-mentioned technology, the paramagnetic steel plate be anchored on outside of deivce face up and down everywhere,
Tight closure plays the role of keeping magnetic line of force circulation therein.
As the preferred embodiment of above-mentioned technology, the diamagnetic aluminium sheet is anchored at the front and back two of device, is close to paramagnetic steel
Plate, it is diamagnetic to prevent the magnetic line of force short-circuit.
As the preferred embodiment of above-mentioned technology, the paramagnetic screw rod fits device outer closures system for fixation
Paramagnetic steel plate and diamagnetic aluminium sheet.
As the preferred embodiment of above-mentioned technology, the diamagnetic cylindrical pins cross paramagnetic steel plate and strong permanent magnetic iron, fixed
Magnet, and be easily installed.
As the preferred embodiment of above-mentioned technology, the device portal is the entrance that crystallization plates are placed, and is resisted before being located at device
The middle of magnetic aluminium sheet.
As the preferred embodiment of above-mentioned technology, the entrance door closure is used for blocking device entrance, strong magnetic in anti-locking apparatus
Environment ferromagnetic material is drawn, is generated dangerous.
It is as shown in figures 1 to 6 a kind of permanent magnetism enclosed experiment device appearance structural schematic diagram for crystallization of protein, dress
Setting four faces up and down is paramagnetic steel plate, and front and back two sides is diamagnetic aluminium sheet, and the integrated connection of device is completed by paramagnetic screw rod,
It is connected firmly, device compact overall structure is secured.
As Fig. 2, Fig. 3, Fig. 4 and Fig. 5 show a kind of permanent magnetism enclosed experiment device internal structure for crystallization of protein
Schematic diagram, that is, device closed test system mainly have each two blocks strong permanent magnetic iron arranged side by side up and down and intermediate U-shaped diamagnetic aluminium
Plate is the rest area as crystallization in the U-shaped inner space of U-shaped aluminium sheet.
Preferably, the size of the experimental provision entirety is 252*212*127mm, and spatial volume is small, can be convenient in this way
Put into water bath with thermostatic control, carry out isothermal experiments.
Preferably, described four blocks diamagnetic steel plates 1.2,1.7,2.1 and 2.5 with a thickness of respectively 30mm, material q235
The magnetic line of force of permanent magnet can be all enclosed in steel plate by steel substantially in this way, and leakage field is few, considerably increase examination inside device
Test the magnetic field strength and the integrally-built intensity of device in area.
Preferably, the diamagnetic aluminium sheet 1.6 and 3.1 is the duralumin plate of thickness 15mm, and aluminium sheet has diamagnetism, device front and back
Short circuit can be recycled in device surrounding to avoid the magnetic line of force using diamagnetic aluminium sheet, thus the magnetic field strength inside enhancement device.
Preferably, the paramagnetic screw rod 1.3 and 1.4, material are paramagnetic steel material q235, and magnetic conduction effect is good, and intensity is big, even
Connect fastening.Big 1.3 diameter of paramagnetic screw rod is 8mm, and for fixing the overall structure of device, small 1.4 diameter of paramagnetic screw rod is
4mm is mainly used for before entrance door closure 1.5 to be blocked to device portal 3.2 and is fixed on diamagnetic aluminium sheet 1.6.
Preferably, the diamagnetic air space 2.4 is located between strong permanent magnetic iron and left and right paramagnetic steel plate, it is possible to reduce magnetic force
Line, directly through paramagnetic steel plate, reduces magnetic line of force loss from magnet two sides.
Preferably, the U-shaped aluminium sheet is clipped between strong permanent magnetic iron up and down, and aluminium sheet is diamagnetic material, and the magnetic line of force is substantially not
It can or can seldom be passed through from U-shaped aluminium sheet, the magnetic line of force is made more to concentrate on the U-shaped indentation, there of U-shaped aluminium sheet in this way, increase crystallization
The magnetic field strength of plate rest area.
Preferably, the specification of the strong permanent magnet 2.6,2.8,5.1 and 5.2 is 110*110*27.5mm, remanent magnetism 1.40
~1.44T, material are ndfeb magnet, and upper and lower distance is 18mm, and the two poles of the earth N-S are opposite, can increase magnet as much as possible in this way
The magnetic field strength at the two poles of the earth.
Preferably, the U-shaped chisel of aluminium sheet locating slot 6.1 is in left and right paramagnetic steel plate 1.7,2.2 and rear diamagnetic aluminium sheet 3.1
Portion primarily serves the effect of limitation and fixed U-shaped aluminium sheet.
Preferably, the chisel of strong permanent magnetic iron locating slot 6.2 compares strong permanent magnetic in upper and lower 2.1,2.5 middle part of paramagnetic steel plate, width
Iron is slightly wide, plays the role of phase magnet.
After the design parameter involved in the present invention arrived and making material determine, finite element analysis software has been used first
ANSYS is simulated experimental analysis, and sunykatuib analysis result crystallization plates rest area is essentially stationary magnetic field, and field strength is greater than 1T.Dress
It sets after completing, is measured using magnetic field strength measuring device, the selected point result of measurement is similar to analog result, magnetic field
Intensity is consistent with desired design result in 1T or so.
The present invention use when the following steps are included:
The first step, crystallization plates point sample.By crystallization of protein solution, point is added on crystallization plates, and crystallization plates carry out relevant treatment.
Second step, crystallization plates are placed.The entrance door closure of apparatus of the present invention is removed with screwdriver, it will be with liquid crystallization plates from dress
Flat propulsion device at posting port, the crystallization plates rest area for being placed in crystallization plates inside device.
Third step lays flat device into circulator bath after fixing device portal door closure, thermostatical crystallization.
4th step when need to observe crystallization effect, takes out crystallization plates, and microscopically observation is taken pictures.
Claims (7)
1. a kind of permanent magnetism enclosed experiment device for crystallization of protein, including permanent magnet, U-shaped aluminium sheet, diamagnetic aluminium sheet and paramagnetic
Steel plate, it is characterised in that: permanent magnet described in two pieces is placed in parallel, and the polarity of two block permanent magnet corresponding positions is opposite；It is described
U-shaped aluminium sheet between two block permanent magnets, aluminium sheet and permanent magnet enclose that five bandings close, the crystallization plates of openings at one side are put
Set area；Several paramagnetic steel plates and diamagnetic aluminium sheet enclose an enclosed construction, permanent magnet and U-shaped aluminium sheet are wrapped in, it is described
Enclosed construction in, the two sides of face U-shaped aluminium sheet opening are diamagnetic aluminium sheet.
2. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: it is described forever
Magnet uses neodymium iron boron strong permanent magnetic iron.
3. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: the U
Respectively there are two block permanent magnets in type aluminium sheet two sides, and two block permanent magnets of the same side, which are generally aligned in the same plane, is adjacent to placement, and two block permanent magnets
The polarity of adjacent side is opposite.
4. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: it is described forever
There are spaces between enclosed construction in the plane locating for itself for magnet.
5. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: the envelope
It closes in structure, the position of the diamagnetic U-shaped aluminium sheet opening of aluminium sheet face is provided with crystallization plates entrance, and is closed by door closure.
6. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: described is suitable
Magnetic steel plate is fastenedly connected by paramagnetic screw rod, and the diamagnetic aluminium sheet is mounted on paramagnetic steel plate by paramagnetic screw rod.
7. the permanent magnetism enclosed experiment device according to claim 1 for crystallization of protein, it is characterised in that: it is described forever
Magnet is mounted on paramagnetic steel plate by magnetic cylindrical pins.
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| CN201610898306.2A CN106644626B (en) | 2016-10-14 | 2016-10-14 | A kind of permanent magnetism enclosed experiment device for crystallization of protein |
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| CN201610898306.2A CN106644626B (en) | 2016-10-14 | 2016-10-14 | A kind of permanent magnetism enclosed experiment device for crystallization of protein |
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| CN111999487B (en) * | 2020-08-25 | 2023-03-28 | 思远(广东)工程技术有限公司 | Permanent magnet closed experimental device for protein crystallization |
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| US5908578A (en) * | 1995-12-07 | 1999-06-01 | Bridgestone Corporation | Bonded magnet-forming composition and magnet roller using the same |
| CN1237550C (en) * | 2001-08-24 | 2006-01-18 | 柏林心脏公开股份有限公司 | Magnetically hard object and method for adjusting the direction and position of a magnetic vector |
| US6954128B2 (en) * | 2001-11-30 | 2005-10-11 | The Regents Of The University Of California | High performance hybrid magnetic structure for biotechnology applications |
| WO2013188725A1 (en) * | 2012-06-14 | 2013-12-19 | President And Fellows Of Harvard College | Levitation of materials in paramagnetic ionic liquids |
| CN103207232B (en) * | 2013-03-12 | 2015-11-18 | 西北工业大学 | A kind of device utilizing cycle pulsating field Study on Protein crystallization |
| CN105823662A (en) * | 2016-04-21 | 2016-08-03 | 天津宏华焊研机器人科技有限公司 | Magnetic treatment device with mixed magnetic field |
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