CN106636224A - Process for producing ethanol through fermentation of maize straws - Google Patents

Process for producing ethanol through fermentation of maize straws Download PDF

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Publication number
CN106636224A
CN106636224A CN201611132489.3A CN201611132489A CN106636224A CN 106636224 A CN106636224 A CN 106636224A CN 201611132489 A CN201611132489 A CN 201611132489A CN 106636224 A CN106636224 A CN 106636224A
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CN
China
Prior art keywords
water
maize straw
saccharification
fermentation
maize straws
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611132489.3A
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Chinese (zh)
Inventor
梁文燊
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Beihai Guangxi Hao Bang Amperex Technology Ltd
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Beihai Guangxi Hao Bang Amperex Technology Ltd
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Priority to CN201611132489.3A priority Critical patent/CN106636224A/en
Publication of CN106636224A publication Critical patent/CN106636224A/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The invention discloses a process for producing ethanol through fermentation of maize straws. The process comprises the following steps: raw material pretreatment, namely crushing maize straws, carrying out acidizing treatment on the maize straws by using a sulfuric acid solution at 100-110 DEG C and treating the maize straws treated by the acid by using a sodium hydroxide solution at 80 DEG C; preparation of a saccharified liquid, namely putting the maize straws into a saccharification container, adding water to the saccharification container, adjusting the pH value of water to 5.1-5.3 by using sulfuric acid, adding cellulose to the water, and then inputting the maize straws and the water into a saccharification reaction kettle; and fermentation, namely putting the activated yeast into the reaction kettle, sealing and fermenting the yeast again, carrying out fermentation reaction for 48h and sampling the product to determine the concentration of ethanol. Hemicellulose and lignin of the maize straws pretreated by acid and alkali are removed, the cellulose content is improved, the saccharification efficiency and the wine-making efficiency are improved, the ethanol yield is greatly improved and the process has relatively good economic benefits.

Description

A kind of technique of maize straw fermentative production of ethanol
Technical field
The present invention relates to renewable new energy source technical field, especially, is related to a kind of work of maize straw fermentative production of ethanol Skill.
Background technology
In the last few years, occurs energy crisis in world wide, environmental pollution is on the rise, people have to find new green The color energy is replacing fossil fuel.In numerous new forms of energy, biomass energy is favored always by people, is produced according to measuring and calculating global year About 150,000,000,000 tons of biomass, including plant base starch, agricultural wastes and lignocellulosic etc..In China, annual crops Stalk yield has 700,000,000 tons or so, domestic also day by day surging to the research of straw utilization because straw utilization rate is low, it is serious to waste. How using biomass fuel to be so the direction that industry is worth studying.
The content of the invention
Present invention aim at providing a kind of technique of maize straw fermentative production of ethanol, comprise the following steps：
Pretreatment of raw material：Corn stalk powder is broken to into 2-5cm length, using 2% sulfuric acid solution at a temperature of 100-110 DEG C Acidification, acidificatoin time 45-50 minutes are carried out to maize straw；
Acid treated maize straw processes 60-65 point with the sodium hydroxide solution of 1.2%-1.5% at a temperature of 80 DEG C again Clock；
It is prepared by saccharified liquid：Maize straw is put in saccharification vessel, by weight 1 part of maize straw, the ratio that 9 parts of water, Water is put in saccharification vessel, the pH value of water body is adjusted to 5.1-5.3 using the sulfuric acid of 1.1%-1.2%, then, added in water fine The plain enzyme of dimension；
After adding cellulase, maize straw and water are input into saccharification reactor, the saccharification reaction under 50 DEG C of isoperibol - 84 hours 72 hours；
Fermentation：The yeast for having activated is put in reactor, fermentation is resealed, ethanol is measured by sampling after fermentation reaction 48h dense Degree.
Further, the step of activated yeast is dissolved in deionized water to take glucose, adds wine brewing high activity to do Yeast, shakes up, tampon sealing, is placed in standing 3-4 hours in 30 DEG C of constant temperature biochemical cultivation case and is activated.
Further, during the activated yeast, by weight 3 parts of glucose, 100 parts of deionized water, wine brewing high activity 0.6 part of dry ferment is added.
The invention has the advantages that：
The Technique of Hemicellulose from Cornstalk and lignin of Jing acid-base pretreatments is removed, and content of cellulose is improved, and saccharification and product wine are imitated Rate increases, and ethanol production is greatly improved, with preferable economic benefit.
Specific embodiment
Embodiments of the invention are described in detail below.
The preferred embodiments of the present invention provide a kind of technique of maize straw fermentative production of ethanol, comprise the following steps：
Pretreatment of raw material：Corn stalk powder is broken to into 2-5cm length, using 2% sulfuric acid solution at a temperature of 100-110 DEG C Acidification, acidificatoin time 45-50 minutes are carried out to maize straw；
Acid treated maize straw processes 60-65 point with the sodium hydroxide solution of 1.2%-1.5% at a temperature of 80 DEG C again Clock；
It is prepared by saccharified liquid：Maize straw is put in saccharification vessel, by weight 1 part of maize straw, the ratio that 9 parts of water, Water is put in saccharification vessel, the pH value of water body is adjusted to 5.1-5.3 using the sulfuric acid of 1.1%-1.2%, then, added in water fine The plain enzyme of dimension；
After adding cellulase, maize straw and water are input into saccharification reactor, the saccharification reaction under 50 DEG C of isoperibol - 84 hours 72 hours；
Fermentation：The yeast for having activated is put in reactor, fermentation is resealed, ethanol is measured by sampling after fermentation reaction 48h dense Degree.
The Technique of Hemicellulose from Cornstalk and lignin of Jing acid-base pretreatments is removed, and content of cellulose is improved, and is saccharified and produces Wine efficiency increases, and ethanol production is greatly improved, with preferable economic benefit.
Preferably, the step of activated yeast is dissolved in deionized water to take glucose, adds the dry ferment of wine brewing high activity Mother, shakes up, tampon sealing, is placed in standing 3-4 hours in 30 DEG C of constant temperature biochemical cultivation case and is activated.
Preferably, during the activated yeast, by weight 3 parts of glucose, 100 parts of deionized water, wine brewing high activity are done 0.6 part of yeast is added.
The preferred embodiments of the present invention are the foregoing is only, the present invention is not limited to；For the skill of this area For art personnel, the present invention can have various modifications and variations.It is all within the spirit and principles in the present invention, made any repair Change, equivalent, improvement etc., should be included within the scope of the present invention.

Claims (3)

1. a kind of technique of maize straw fermentative production of ethanol, it is characterised in that comprise the following steps：
Pretreatment of raw material：Corn stalk powder is broken to into 2-5cm length, using 2% sulfuric acid solution at a temperature of 100-110 DEG C Acidification, acidificatoin time 45-50 minutes are carried out to maize straw；
Acid treated maize straw processes 60-65 point with the sodium hydroxide solution of 1.2%-1.5% at a temperature of 80 DEG C again Clock；
It is prepared by saccharified liquid：Maize straw is put in saccharification vessel, by weight 1 part of maize straw, the ratio that 9 parts of water, Water is put in saccharification vessel, the pH value of water body is adjusted to 5.1-5.3 using the sulfuric acid of 1.1%-1.2%, then, added in water fine The plain enzyme of dimension；
After adding cellulase, maize straw and water are input into saccharification reactor, the saccharification reaction under 50 DEG C of isoperibol - 84 hours 72 hours；
Fermentation：The yeast for having activated is put in reactor, fermentation is resealed, ethanol is measured by sampling after fermentation reaction 48h dense Degree.
2. the technique of a kind of maize straw fermentative production of ethanol according to claim 1, it is characterised in that：
The step of activated yeast, is dissolved in deionized water to take glucose, adds highly active dry yeast, is shaken up, tampon Sealing, is placed in standing 3-4 hours in 30 DEG C of constant temperature biochemical cultivation case and is activated.
3. the technique of a kind of maize straw fermentative production of ethanol according to claim 2, it is characterised in that：
During the activated yeast, by weight 3 parts of glucose, 100 parts of deionized water, 0.6 part of highly active dry yeast enter Row addition.
CN201611132489.3A 2016-12-09 2016-12-09 Process for producing ethanol through fermentation of maize straws Pending CN106636224A (en)

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Application Number Priority Date Filing Date Title
CN201611132489.3A CN106636224A (en) 2016-12-09 2016-12-09 Process for producing ethanol through fermentation of maize straws

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107354177A (en) * 2017-07-10 2017-11-17 农业部沼气科学研究所 A kind of anaerobic fermentation method of stalk
CN109022517A (en) * 2017-06-09 2018-12-18 华东理工大学 A kind of parameter regulation means for eliminating different lignocellulosic material pretreatment efficiency variances
CN111048809A (en) * 2019-12-13 2020-04-21 沈阳航空航天大学 Hydrogen-oxygen fuel cell intelligent power generation system based on hydrogen production by straw ethanol fermentation and reforming

Citations (4)

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CN101063154A (en) * 2007-05-23 2007-10-31 中国林业科学研究院亚热带林业研究所 Method for producing fuel ethanol by using chestnut as raw material
CN104593448A (en) * 2014-12-25 2015-05-06 山东龙力生物科技股份有限公司 Method for producing ethanol from lignocellulosic biomass
CN104651414A (en) * 2013-11-16 2015-05-27 西安交大京盛科技发展有限公司 Pretreatment method for preparing ethanol fuel by straw
CN104846034A (en) * 2015-05-14 2015-08-19 天津大学 Method for improving yield of bioethanol prepared by recycling and reusing cellulose

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101063154A (en) * 2007-05-23 2007-10-31 中国林业科学研究院亚热带林业研究所 Method for producing fuel ethanol by using chestnut as raw material
CN104651414A (en) * 2013-11-16 2015-05-27 西安交大京盛科技发展有限公司 Pretreatment method for preparing ethanol fuel by straw
CN104593448A (en) * 2014-12-25 2015-05-06 山东龙力生物科技股份有限公司 Method for producing ethanol from lignocellulosic biomass
CN104846034A (en) * 2015-05-14 2015-08-19 天津大学 Method for improving yield of bioethanol prepared by recycling and reusing cellulose

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
郑国香: "《能量微生物学》", 31 July 2013, 哈尔滨工业大学出版社 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109022517A (en) * 2017-06-09 2018-12-18 华东理工大学 A kind of parameter regulation means for eliminating different lignocellulosic material pretreatment efficiency variances
CN109022517B (en) * 2017-06-09 2021-09-28 华东理工大学 Parameter adjustment method for eliminating difference of pretreatment efficiency of different lignocellulose raw materials
CN107354177A (en) * 2017-07-10 2017-11-17 农业部沼气科学研究所 A kind of anaerobic fermentation method of stalk
CN107354177B (en) * 2017-07-10 2020-12-01 农业部沼气科学研究所 Anaerobic fermentation method of straw
CN111048809A (en) * 2019-12-13 2020-04-21 沈阳航空航天大学 Hydrogen-oxygen fuel cell intelligent power generation system based on hydrogen production by straw ethanol fermentation and reforming

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