CN106636030A - Plant drought resistance associated protein EtSnRK2.2 and coding gene and application thereof - Google Patents
Plant drought resistance associated protein EtSnRK2.2 and coding gene and application thereof Download PDFInfo
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- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
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Abstract
The invention relates to the field of gene engineering, specifically to a plant drought resistance associated protein EtSnRK2.2 and a coding gene and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO. 1, and the gene sequence of the protein is shown as SEQ ID NO. 2. The drought resistance associated protein and the coding gene of the protein can improve stress resistance of wheat, increase the yield, accelerate a stress-resistant molecular breeding process, save water resources, and have great theoretical and practical significance.
Description
Technical field
The present invention relates to genetic engineering field, and in particular to plant drought GAP-associated protein GAP EtSnRK2.2 and its encoding gene
And application.
Background technology
Wheat occupies very important status as one of important cereal crops of China in national economy.However, every
Year drastically influence the yield and quality of wheat because of the environment stress condition such as arid, saline and alkaline, govern China's wheat grain security.
The relation between plant and abiotic stress is furtherd investigate from molecular level using technique for gene engineering, plant is disclosed to adverse circumstance
Stress signal conducts and gene expression regulation molecule mechanism, clones anti contravariance related because cultivating the degeneration-resistant new germ plasm of crop provides time
Select adversity gene resource.
Sucrose non-fermented related protein kinase enzyme family (SnRKs) plays important work in many physiology courses of plant
With such as hormone signal conduction, abiotic stress and plant are grown.SnRK2 family genes functionally show
Otherness, has 9 genes to be induced by mannitol or NaCl hyperosmotic stress in SnRK family members in arabidopsis, 5 genes are by ABA
Induction, but do not receive induction of chilling stress.SnRK genes in paddy rice, by protein phosphorylation analysis shows, all members can be high
Stress activation is oozed, but only these three genes of OsSAPK8, OsSAPK9 and OsSAPK10 receive ABA abduction deliverings.In wheat,
First SnRK2 member is isolated PKABA1 from the wheat embryo cDNA library of ABA process, and the expression of PKABA1 is removed
Induced by ABA and drought stress.TaSnRK2.4 gene overexpressions can strengthen transgenic arabidopsis to arid, the side of body of high salt
Urgent patience, while not resulting in the growth dwarfism of plant.TaSnRK2.7 gene function analysis show, in glycometabolism, reduce
Play an important role in the physiological and biochemical procedures such as osmotic potential, the activity for strengthening Photosystem I I and promotion plant establishment；
The arabidopsis of TaSnRK2.8 gene overexpressions has certain stress tolerance to arid, low temperature, high salt, high temperature etc..
Therefore, the resistance for clone, separating degeneration-resistant related SnRK protein kinase genes improvement and improve crop has very
Important meaning.
The content of the invention
It is an object of the invention to provide a kind of plant drought GAP-associated protein GAP EtSnRK2.2.
Another object of the present invention is to provide the gene of the above-mentioned plant drought correlation egg EtSnRK2.2 of coding.
It is a further object of the present invention to provide the recombinant vector comprising said gene.
It is a further object of the present invention to provide the transgenic cell line comprising said gene.
Another object of the present invention provides the application of above-mentioned plant drought GAP-associated protein GAP EtSnRK2.2.
Drought resistant correlative protein EtSnRK2.2 provided by the present invention, from hair fringe couchgrass, its amino acid sequence is such as
Shown in SEQ ID NO.1.
The protein kinase of the present invention is made up of 343 amino acid residues, is SnRK albuminoid kinases.From SEQ ID NO.1
Amino terminal 10-30 amino acids residues be ATP binding domain, from the 115-130 amino acids residues of SEQ ID NO.1
For serine/threonine binding domain.
SEQ ID NO：1
In order that albumen EtSnRK2.2 is easy to purifying, the egg that can be constituted in the amino acid sequence shown in SEQ ID NO.1
The amino terminal of white matter or the upper label as shown in table 1 of carboxyl terminal connection.
The sequence of the label of table 1
| Label | Residue | Sequence |
| Poly-Arg | 5-6 (usually 5) | RRRRR |
| Poly-His | 2-10 (usually 6) | HHHHHH |
| FLAG | 8 | DYKDDDDK |
| Strep-tag II | 8 | WSHPQFEK |
| c-myc | 10 | EQKLISEEDL |
According to SEQ ID NO.1 sequences disclosed in this invention, transcription factor EtSnRK2.2 of the present invention can be closed manually
Into also can first synthesizing its encoding gene, then carry out biological expression and obtain.
EtSnRK2.2 encoding genes of the invention have the cDNA sequence as shown in SEQ ID NO.2.
SEQ ID NO.2
Expression cassette containing EtSnRK2.2 genes, recombinant expression carrier, transgenic cell line and recombinant bacterium belong to this
Bright protection domain.
The recombinant expression carrier of EtSnRK2.2 genes can be contained with existing plant expression vector construction.
The plant expression vector includes double base agrobacterium vector and can be used for carrier of plant micropellet bombardment etc..The plant
Thing expression vector can also include 3 ' end untranslated regions of foreign gene, i.e., comprising polyadenylation signals and any other participation
MRNA processing or the DNA fragmentation of gene expression.The bootable polyadenylic acid of the polyadenylation signals is added to the 3 ' of mRNA precursor
End, such as Agrobacterium crown gall nodule induction (Ti) plasmid gene (such as kermes synzyme Nos genes), non-the turning over of the end of plant gene 3 ' transcription
Translate area and be respectively provided with similar functions.
When building recombinant plant expression vector using EtSnRK2.2, any one can be added before its transcription initiation nucleotides
Plant enhancement mode promoter or constitutive promoter, such as cauliflower mosaic virus (CaMV) 35S promoter, the ubiquitin promoter of corn
(Ubiquitin), they can be used alone or are used in combination with other plant promoters；Additionally, using the gene structure of the present invention
When building plant expression vector, enhancer, including translational enhancer or transcriptional enhancer are it is also possible to use, these enhancer regions can be with
It is ATG initiation codon or neighboring region initiation codon etc., but must be identical with the reading frame of coded sequence, it is whole to ensure
The correct translation of sequence.The source of the translation control signal and initiation codon is extensive, can be natural, it is also possible to
It is synthesis.Translation initiation region can come from transcription initiation region or structural gene.
For the ease of transgenic plant cells or plant are identified and screened, plant expression vector used can be carried out
Processing, such as add the coding that can be expressed in plant can produce color change enzyme or luminophor gene (gus gene,
Luciferase genes etc.), the antibiotic marker (gentamicin label, kanamycins label etc.) with resistance or anti-
Chemical reagent marker gene (such as anti-herbicide gene).From the security consideration of genetically modified plants, any selectivity can be not added with
Marker gene, directly screens transformed plant with adverse circumstance.
It is a further object to provide a kind of method for cultivating plant with adverse resistance.
The method for cultivating plant with adverse resistance provided by the present invention, is by any of the above-described kind of weight containing EtSnRK2.2 genes
Group expression vector is imported in plant cell, obtains plant with adverse resistance.
The carrier that foreign gene expresses in plant can be guided using any one, by SnRK eggs provided by the present invention
White kinases EtSnRK2.2 gene transfered plant cells, can obtain enhanced to abiotic stress tolerances such as arid and salt
Transgenic cell line and transfer-gen plant.The expression vector for carrying encoding gene can be by using Ti-plasmids, Ri plasmids, plant
The conventional biology methods such as viral vector, directly delivered DNA, microinjection, conductance, agriculture bacillus mediated convert plant cell or group
Knit, and the plant tissue of conversion is cultivated into into plant.The plant host being converted both can be monocotyledon, or double
Cotyledon plant, such as：Arabidopsis, wheat, hair fringe couchgrass, arabidopsis, paddy rice, corn, cucumber, tomato, willow, turfgrass, lucerne
Place etc..
The present invention, as experiment material, is obtained with the stronger hair fringe couchgrass of drought resistance (Elytrigia trichophora L.)
The EtSnRK2.2 albumen and its encoding gene of degeneration-resistant correlation are arrived, and has been conducted into wheat, significantly improved transgenic wheat
Drought resistance.The drought resistant correlative protein and its encoding gene of the present invention is to improvement, enhancing Resistance of Wheat To Adversity, and raising yield, acceleration are anti-
Against molecular breeding process, and effectively saving water resource has highly important theoretical and practical significance.
Below in conjunction with the accompanying drawings and specific embodiment the present invention will be further described.
Description of the drawings
Fig. 1 shows EtSnRK2.2 transgenic wheat Molecular Detection results.M：Trans2K Plus DNAmarker；7：It is cloudy
Property control；2-6 is different transgenic wheat strains.
Fig. 2 shows EtSnRK2.2 transgenic wheats drought canopy drought resisting qualification result, wherein, the capital winter 18 is acceptor control；446-
Isosorbide-5-Nitrae 63-2,660-1,471-3 are different transgenic lines.
Fig. 3 shows EtSnRK2.2 transgenic wheats field Physiological Indices of Drought Resistance measurement result, mainly determines 446-1,
463-2,660-1,471-3 transgenic line and the soluble sugar and chlorophyll fluorescence in acceptor capital winter 18.
Fig. 4 show EtSnRK2.2 transgenic wheats difference strain phenotype compare, mainly investigated 421-1,437-8,
The data such as grain number per spike, plant height, the spike length in 471-2,582-1 transgenic line and acceptor capital winter 18.
Specific embodiment
The experimental methods of molecular biology for illustrating, equal reference are not made in following examples《Molecular Cloning:A Laboratory guide》
Listed concrete grammar is carried out in the book of (third edition) J. Pehanorm Brookers one, or is carried out according to kit and product description.
Below example facilitates a better understanding of the present invention, but does not limit the present invention.
Embodiment 1：The cDNA clone of hair fringe couchgrass drought resisting correlation EtSnRK2.2 genes
Osmotic treatment is carried out 5 hours to the growth hair fringe couchgrass seedling of 30 days or so, hair fringe is extracted with Trizol and is laid down wheat
Careless total serum IgE.Using 5 ' RACE kits (GIBCOBRL, CAT.NO.18374-058) and 3 ' RACE kits (GIBCOBRL,
CAT.NO.18373-019 the full length sequence 1032bp of EtSnRK2.2 genes) is obtained.
The total serum IgE of hair fringe couchgrass seedling is extracted with Trizol, with superscript II (invitrogen) reverse transcription
Enzyme reverse transcription acquires cDNA.Primer P1 and P2 are designed according to EtSnRK2.2 coding sequences.Obtained with reverse transcription
CDNA is template, and with primer P1 and P2 performing PCR amplification is entered.The sequence of primer P1 and P2 is as follows：
P1：5 '-ATGGATCGGTACGAGGTGGT-3 ',
P2：5’-TTACAAAGGGCACACGAAATCC-3’.
0.8% agarose gel electrophoresis detection is carried out to PCR primer, the band that molecular weight is about 1kb or so is obtained, it is and pre-
Phase result is consistent.The fragment is reclaimed, the recovery fragment is connected with pGEM-T Easy (Promega), then convert Escherichia coli
DH5 α competent cells, according to the acillin resistance marker screening positive clone on pGEM-T Easy carriers, are contained
Reclaim the recombinant plasmid of fragment.With T7 the and SP6 promoter sequences on the recombinant plasmid vector as primer pair, it carries out nucleotides
Sequencing, sequencing result shows that the ORFs (ORF) of the EtSnRK2.2 genes for expanding is oneself of SEQ ID No.2
5 ' the deoxyribonucleotides of end the 1st to 1032, encoding amino acid sequence is the protein of SEQ ID No.1.Sequence will be contained
The recombinant vector of EtSnRK2.2 genes is named as pTE-EtSnRK2.2 shown in SEQ ID No.2.
EtSnRK2.2 genes are a new gene, are further carried out in hair fringe couchgrass genome with primer P1 and P2
Amplification, as a result shows that the genome sequence size of the gene is consistent with cDNA length scales, does not contain intron sequences.
Embodiment 2：With the drought resistance of EtSnRK2.2 genes amplification plants
1st, the structure of recombinant expression carrier
1) structure of Ubi-EtSnRK2.2 recombinant expression carriers
The cDNA obtained with the total serum IgE reverse transcription of hair fringe couchgrass as template, with containing HindIII and BamHI joint sequences
The special primer of row enters performing PCR amplification；Then HindIII and BamHI double digestions PCR primer is reclaimed, and digestion products are positive slotting
Enter between HindIII the and BamHI restriction enzyme sites after the Ubi promoters of carrier pNT112, obtain recombinant vector Ubi::
EtSnRK2.2。
Primer sequence is as follows：
EtSnRK2.2[HindIII]5’-TCAAGCTTATGGATCGGTACGAGGTGGT-3’
EtSnRK2.2[BamHI]5’-GG GGATCCTTACAAAGGGCACACGAAATCC-3’
2nd, transgenic wheat is obtained and Function Identification
1) acquisition of transgenic wheat material
By the recombinant expression carrier pUbi of above-mentioned structure::EtSnRK2.2 converts Agrobacterium tumefaciems with freeze-thaw method respectively
C58C1, then use pUbi::The Agrobacterium tumefaciems C58C1 transformed wheats of EtSnRK2.2, are cultivated with the MS containing 200mg/L Basta
Base is screened, and obtains positive transgenic plant.The positive transgenic plant that screening is obtained PCR does further identification sieve
Choosing, the pair of primers used by PCR is P3 and P4.
P3 (upstream primer):5 '-GGAGATTATGAACCACCGGT-3 ',
P4 (downstream primer):5’-TCTCCGGGATGGTTATTCGC-3’.
To Ubi::EtSnRK2.2 transgenic wheats enter performing PCR identification, and positive transgenic plant Jing PCR amplifications can be obtained
1000bp or so bands, as a result obtain and turn Ubi::56 plants of EtSnRK2.2 wheats (Fig. 1).
Simultaneously pNT112 empty carriers are imported into wheat, method ibid, as control, obtain 10 strains to turn empty carrier little
Wheat, screens the transgenic wheat T for obtaining2Representative is shown.
2) EtSnRK2.2 transgenic wheats Identification of Drought
In the case of the time of infertility only pours water of turning green, the EtSnRK2.2 transgenic lines to planting carry out non-irrigated canopy drought resistance
Screening.Drought resisting phenotypic evaluation in field is found, EtSnRK2.2 transgenic lines hold green property preferably, and boot leaf remains able to normally enter
Row photosynthesis, kernel grouting is sufficient, and mass of 1000 kernel increases notable；And compareing the capital winter 18, to hold green property poor, boot leaf and other position leaves
Piece jaundice, early ageing are serious, it is impossible to be normally carried out photosynthesis (Fig. 2).Soluble sugar, chlorophyll fluorescence physiological index determining are sent out
Existing, Fluorometer, the soluble sugar content of EtSnRK2.2 transgenic lines accumulate more (Fig. 3) than control.Additionally,
Find from the species test data analysis for harvesting, the proterties such as grain number per spike, root system, spike length of transgenic line is superior to control, so that
Obtain EtSnRK2.2 transgenic line drought tolerances to significantly increase (Fig. 4).
<110>Beijing City Agriculture and Forestry Institute
<120>Plant drought GAP-associated protein GAP EtSnRK2.2 and its encoding gene and application
<160>2
<210> 1
<211> 343
<212> PRT
<213>Couchgrass
<400> 1
MDRYEVVRDI GSDNFGVAKL VRDVRTKEHF AVKFIKRGRK IDEHVQREIM NHRSLKHPNI 60
IRFKEVVLTP THLAIIMEYA SGGELFQRIC NAGRFSEDEG RFFFQQLISG VSYCHSMQVC 120
HRDLKLENTL LDGSVAPRLK ICGFGYSKSS VLHSQPKSTV GTPAYIAPEV LSRREYDGKV 180
ADVWSCGVTL YVMLVGAYPF EDPDEPRNFR KTITRILSVQ YSVPDYVRVS MDCIHLLSRI 240
FVGNPQQRIT IPEIKNHPWF LKRLPVEMTD EYQRSMQLAD MNTPSQSLEE AMAIIHEARK 300
PGDSALGIAG QVARLGSMDL DDIDFDDIDD IDIENSGDFV CPL 343
<210> 2
<211> 1032
<212> DNA
<213>Couchgrass
<400> 2
atggatcggt acgaggtggt gagggacatc gggtccgaca acttcggggt ggcgaagctg 60
gtgcgggacg tcaggaccaa ggagcacttc gccgtcaagt tcatcaagcg aggccgcaag 120
attgatgaac atgttcaaag ggagattatg aaccaccggt cactcaagca tccaaatatt 180
atccgattca aggaggtcgt gctaactccc acacatttgg caataattat ggaatatgcc 240
tctgggggcg agctatttca aaggatttgc aacgcaggga gatttagcga ggatgaggga 300
aggttcttct tccaacaatt gatttctgga gtgagctatt gtcactctat gcaagtatgt 360
catagagatt tgaaactaga gaatactctc ttggatggta gtgttgcacc tcgactcaag 420
atttgtggct tcggttactc caagtcttct gtcttgcatt ctcaaccgaa gtcaactgtt 480
ggcacaccgg catacatcgc cccggaggtc ctctctagaa gagagtatga tggaaaggtc 540
gctgatgttt ggtcttgtgg agtaacgctc tatgtgatgc ttgtcggggc atatcctttc 600
gaggaccctg atgagccaag gaacttccgc aaaacaatca ctaggatact cagtgtacag 660
tactctgttc cggactacgt tcgagtctcc atggattgca tacatctgct gtcccgcatt 720
ttcgttggaa atcctcagca gcgaataacc atcccggaga tcaagaacca tccatggttc 780
ctcaaacgcc tgcccgttga gatgaccgat gagtaccaaa ggagcatgca gttggcagac 840
atgaacacgc cgtcacagag cctggaagaa gccatggcga tcatccatga ggcacggaaa 900
ccgggtgata gcgccctagg gattgctggg caggttgccc gcctggggag catggatcta 960
gatgacattg atttcgacga tatcgacgac attgacattg agaacagcgg ggatttcgtg 1020
tgccctttgt aa 1032
Claims (8)
1. a kind of plant drought GAP-associated protein GAP EtSnRK2.2, it is characterised in that its amino acid sequence is as shown in SEQ ID NO.1.
2. a kind of gene studies on plant drought-resistance EtSnRK2.2, it is characterised in that the plant drought phase described in coding claim 1
Close albumen EtSnRK2.2.
3. gene studies on plant drought-resistance EtSnRK2.2 as claimed in claim 2, it is characterised in that its base sequence such as SEQ
ID NO.2。
4. the recombinant vector of gene studies on plant drought-resistance EtSnRK2.2 described in Claims 2 or 3 is included.
5. the recombinant bacterial strain of gene studies on plant drought-resistance EtSnRK2.2 described in Claims 2 or 3 is included.
6. the application of plant drought GAP-associated protein GAP EtSnRK2.2 described in claim 1.
7. the application of gene studies on plant drought-resistance EtSnRK2.2 described in Claims 2 or 3.
8. the application in terms of plant drought resistance is improved of plant drought salt GAP-associated protein GAP EtSnRK2.2 described in claim 1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109385487A (en) * | 2018-09-11 | 2019-02-26 | 浙江省食品药品检验研究院 | The recombinase-mediated amplification Constant Temperature Detection method and kit of your detail herbal medicine American Ginseng |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130326734A1 (en) * | 2011-02-01 | 2013-12-05 | The Regents Of The University Of California | Compositions and methods for controlling carbon dioxide- (co2-) regulated stomatal apertures, water transpiration and water use efficiency in plants |
-
2016
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130326734A1 (en) * | 2011-02-01 | 2013-12-05 | The Regents Of The University Of California | Compositions and methods for controlling carbon dioxide- (co2-) regulated stomatal apertures, water transpiration and water use efficiency in plants |
Non-Patent Citations (2)
| Title |
|---|
| LING.H.Q等: "Abscisic acid-inducible protein kinase [Triticum urartu]", 《GENBANK：EMS57069.1》 * |
| 张照贵等: "小麦SnRK2.2基因克隆、表达载体构建及转化", 《山东农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109385487A (en) * | 2018-09-11 | 2019-02-26 | 浙江省食品药品检验研究院 | The recombinase-mediated amplification Constant Temperature Detection method and kit of your detail herbal medicine American Ginseng |
| CN109385487B (en) * | 2018-09-11 | 2022-04-01 | 浙江省食品药品检验研究院 | Recombinase-mediated amplification isothermal detection method and kit for American ginseng as Chinese medicinal herb |
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