CN106635896B - A method of industrialization anaerobic ammonia oxidizing bacteria being stored and utilizing again - Google Patents
A method of industrialization anaerobic ammonia oxidizing bacteria being stored and utilizing again Download PDFInfo
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Abstract
A method of storing and utilizing again for industrialization anaerobic ammonia oxidizing bacteria belongs to biological wastewater treatment field.This method comprises: three selection of Anammox strain, storage and activation recovering steps, step 1 takes out activated sludge from the anaerobic ammonia oxidation reactor different parts operated normally and is uniformly mixed and be filled with high-purity argon gas, choose suitable Anammox strain, after step 2 adds depot matrix after eluriating the strain precipitating of selection, it is filled with high-purity argon gas, the environmental conditions such as temperature and the substrate concentration in storage process are selected according to the different shelf lives.Anammox strain after step 3 pair storage carries out the activation recovering operation of controllable decompression heating, ladder load, can obtain rapidly convenient for the Anammox strain of the high activity of industrialized utilization again.The present invention realizes the high activity storage of anaerobic ammonia oxidizing bacteria, and operations factor is the control condition that industrialization is easily achieved, easy to operate, low-cost, has very high application value.
Description
Technical field
The invention belongs to biological wastewater treatment fields, are related to a kind of storage of anaerobic ammonia oxidizing bacteria and the side utilized again
Method.
Background technique
Anaerobic ammonia oxidation process has been acknowledged as a kind of novel efficient life since nineteen ninety-five is found
Object denitrogenation technology.Since anaerobic ammonia oxidizing bacteria can be with inorganic CO3 -Growth metabolism is carried out as sole carbon source, is not necessarily to organic carbon
Source；Without oxygen supply；Its ammonia oxidation speed rate is 25 times of aerobic nitrification bacterium；The attainable total nitrogen elimination capacity of institute is that tradition nitrification is anti-
The decades of times of nitration processes；Sludge yield is extremely low.Above-mentioned excellent operational characteristic leads Anammox technology in biological denitrificaion
Domain highlights its economy, and has natural adaptability and efficient denitrification ability to the ammonia-containing water of low ratio of carbon to ammonium, make its
Research and industrial application show powerful vitality.
The anaerobic ammonia oxidizing bacteria doubling time is long, grows very slow, growth rate 0.0027h-1, doubling time 10.6d, production
Rate coefficient is 0.07g protein/gNH4 +-N.Various countries researcher furthers investigate the growth factor of anaerobic ammonia oxidizing bacteria,
Its preference temperature is 30~40 DEG C, and pH is 6.7~8.3, and the dosis tolerata of dissolved oxygen is 0.5%, and the tolerable concentration to nitrous acid is
7mmol/L.Anaerobic ammonia oxidizing bacteria extremely low abundance in its natural state, the Retarder theory of growth and the sensibility to environmental condition
Increase the difficulty of its enrichment culture and industrial applications.
Studies have shown that anaerobic ammoniation oxidation technology start run when, increase early period inoculation anaerobic ammonium oxidation sludge amount or
The addition frequency for increasing later period Anammox strain can overcome the application difficult of anaerobic ammonia oxidation process.Enrichment culture is simultaneously effective
Preservation Anammox strain is to solve the deficient effective measures of its application.Common preiservative methods have atoleine method,
Liquid nitrogen method, refrigeration, freezing and gel embedding method etc..The bacterial activity although these technologies can with a hook at the end to a certain extent
Effect, but can the preservation process that directly apply to anaerobic ammonia oxidizing bacteria need to accumulate comparable experimental data and give and support.And
And since height of the anaerobic ammonia oxidizing bacteria to temperature change and substrate concentration is all very sensitive, suitable preservation item is selected
Part and environmental factor are most important.Microbial activity tends towards stability after falling before with the extension of holding time, if the holding time
When shorter, microbial activity will not only be changed using the method that is stored at room temperature and be had an impact, but also cost can be saved；It is longer between when saved
When, the selection of low temperature also needs to be reduced as far as the suppressed degree of activity, while considering the saving of cost.
Activity recovery of the strain after the preservation of certain time is on the one hand related with its storage conditions and environmental factor,
On the other hand also directly related with the mode of activity recovery and process.Culture presevation especially under the conditions of non-room temperature, needs
Consider strain for the adaptability of environment temperature fusion.
Summary of the invention
The present invention provides a kind of sides stored and utilize again to anaerobic ammonia oxidizing bacteria for meeting practical application in industry
Method.It can be relatively simple realization industrial applications in conventional environment factor and technical conditions under to state be suitable for anaerobism
Ammonia oxidation bacteria is chosen, is effectively stored, and carries out more rapid, reliable strain activity recovery, to realize Anammox
The utilization again of strain.Control selector needed for whole operation process is less, and control condition is relatively simple and is to be easy to real
Existing conventional commercial condition, storage and activity recovery effect stability are reliable.The present invention is mainly directed towards Anammox strain
Industrialization storage and sales process, while can effectively realize the seed sludge and starting reactor of industrial applications.
The storage of industrialization anaerobic ammonia oxidizing bacteria of the present invention and the method utilized again are as follows:
1, the selection of Anammox strain
Anammox strain during storage, needs to be in lower temperature and matrix environment condition for a long time,
To maintain lower metabolic activity.Therefore it in order to resisting more severe living environment, needs to being suitable for long time stored detesting
Anaerobic ammonium oxidation strain is screened.
1.1, from the Anammox fluidized-bed reactor of normal operation or up flow anaerobic sludge blanket reactor it is upper,
In, lower three parts take out anaerobic ammonium oxidation sludge respectively, be respectively placed in natural sedimentation 30 in transfer container and divide kind, using siphonage
Be discharged container in supernatant, container bottom precipitated after anaerobic ammonium oxidation sludge.
1.2, the sludge at three positions of reactor respectively obtained in step 1.1 is carried out according to 2: 5: 3 volumetric ratio equal
Even mixing, mixed process are stirred by the way of high-purity argon gas perforated pipe aerating regulation.
1.3, the maximum anaerobic ammoxidation activity for the mixing anaerobic ammoxidation strain that determination step 1.2 obtains, i.e. unit matter
TN removel rate of the Anammox strain of amount under single flow condition.Active survey is carried out using constant temperature batch reactor
Amount.O.15kgTN/kgVSS.d Anammox strain that maximum anaerobic ammoxidation activity is greater than is suitable for being stored.
2, the storage of Anammox strain
2.1, the Anammox strain that will meet shelf appeal is statically placed in Anammox strain hold-up vessel natural
Precipitating 30 minutes, using siphonage discharge container in supernatant, container bottom precipitated after Anammox strain.
2.2, the anaerobic water for being free of matrix is prepared, composition includes nutrient salt solution, inorganic salt solution and tap water；Every liter
It needs that nutrient salt solution 5mL and inorganic salt solution 5ml is added in water distribution without matrix；Nutrient salt solution composition are as follows: NaH2PO4 3
~5g/L, CaCl225~30g/L；Inorganic salt solution composition: FeSO4.7H21~2g/L of O, (NH4)6Mo7O24·4H2O 0.03
~O.05g/L, CuSO4·5H2O O.04~0.06g/L, ZnSO4·6H2O O.05~0.10g/L, MnSO4O.15~
0.25g/L、CoCl2·6H20.04~0.06g/L of O, 3~5g/L of EDTA；By above-mentioned requirements prepared matching without matrix
Water is filled with high-purity argon gas 10~30 minutes using micropore aeration pipe, and measurement oxygen in water concentration stops after being less than 0.2mg/L
Applying argon gas is free of the anaerobic water of matrix to get arriving.
2.3, Anammox strain will be obtained in step 2.1, eluriated with the anaerobic water without matrix in step 2.2,
Natural sedimentation in 30 minutes is carried out later, and supernatant is discharged using siphonage.This elutriation process carries out 3 times altogether, to remove anaerobism ammonia
Aoxidize the matrix and other substances of strain itself institute band.
2.4, clean Anammox strain will be eluriated obtained in step 2.3 to be placed in strain hold-up vessel, detest
Volume of the anaerobic ammonium oxidation strain in strain hold-up vessel accounts for the 40%~50% of strain hold-up vessel volume.It is store later in strain
The anaerobic water without matrix that step 2.2 is added in container is deposited to the 80% of strain hold-up vessel volume, adds ammonium hydrogen carbonate
Solution 1.0g/L and sodium nitrite solution 1.0g/L provides ammonia nitrogen and nitrite nitrogen into strain storage liquid respectively.
The volume of the ammonium bicarbonate soln and sodium nitrite solution that are added is selected by the different shelf lives.In short term
When being stored in 1~30 day, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should make the matrix in strain hold-up vessel
Concentration respectively reaches ammonia nitrogen 5mmol/L, nitrite nitrogen 5mmol/L.
At long-term storage 31~120 days, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should be such that strain stores
Substrate concentration in container respectively reaches ammonia nitrogen 10mmol/L, nitrite nitrogen 10mmol/L.
Addition strain and the strain hold-up vessel of matrix are re-filled with 10 minutes high-purity argon gas, guarantee in strain hold-up vessel
Dissolved oxygen concentration is lower than 0.2mg/L.Later, into strain hold-up vessel, addition is free of matrix while being filled with high-purity argon gas
Anaerobic water covers the sealing of flange seal lid to the 90% of strain hold-up vessel total measurement (volume) later.
2.5, continue to be filled with high-purity argon gas into strain hold-up vessel obtained in step 2.4, reach the pressure in container
To 0.2~0.25MPa.The process of applying argon gas should be steady, slow, controls pressure in the bottle ramp-up rate in 0.01MPa~0.02MP/
In the range of min.Air intake valve is closed later, and checks that strain hold-up vessel seals situation, it is ensured that the sealing of strain hold-up vessel
Performance is intact.
2.6, corresponding storage temperature is selected according to the different shelf lives,
When carrying out short-term anaerobic ammonia oxidizing bacteria storage in 1~30 day, the temperature condition that uses is 20 ± 4 DEG C of room temperature ring
Border.
When carrying out long-term anaerobic ammonia oxidizing bacteria storage in 31~120 days, using thermostatic chamber or freezer, temperature condition is kept
It is 4 ± 1 DEG C.
2.7, during storage, strain hold-up vessel be should be in light protected environment.It is checked daily in strain hold-up vessel
Situation is sealed, by being filled with a small amount of argon gas or opening relief valve, guarantees the pressure in the bottle between 0.2MPa~0.25MPa.
3, the activation recovering of Anammox strain after storing
3.1 by it is quasi- progress strain again using Anammox strain hold-up vessel progress be slowly decompressed to normal pressure, pressure
Power reduces speed control in 0.005MPa~0.01MPa/min.
Strain hold-up vessel after 3.2 decompressions is placed in thermostatic chamber, and warming temperature is carried out.Temperature-rise period is controllable using being passed through
The high-purity argon gas of temperature is stirred heating, and the speed of heating is 1~2 DEG C/h.
The strain of 20 ± 4 DEG C of conditions storage is warming up to 30 DEG C, stands 4~6h.
The strain of 4 ± 1 DEG C of conditions storage should carry out ladder-elevating temperature operation, be warming up to 20 DEG C first, stop heating and stirring
And 20 DEG C of conditions is maintained to stand 4~6h；It is further heated up to 30 DEG C, 4~6h is stood.
Strain after 3.3 heatings uses the anaerobic water without matrix to be eluriated, and argon gas stirs 10min, stands 1~2h
Afterwards, supernatant is excluded using siphonage.It eluriates and carries out 2 times altogether, to remove the residual matrix, metabolite and death on strain surface
The thallus of disintegration.
The 3.4 Anammox strains obtained by step 3.3 can both put into Anammox strain activity recovering device, institute
Stating Anammox strain activity recovering device can be used anaerobic fluidized bed, upflow anaerobic sludge blanket process, batch-type anaerobic reaction
The diversified forms such as device.Gravity precipitation and separation is carried out to the water outlet of Anammox strain activity recovering device simultaneously, sedimentation basin
Hydraulic detention time is 6~8 hours, with the Anammox strain that structure after the long-term storage of retention is more loose.Sedimentation basin is dirty
Mud is manually added to daily in Anammox strain active reactor, to increase the recycling amount of strain.
The main control condition of 3.5 Anammox strain activity recovering devices are as follows: 32~35 DEG C of temperature, pH=7.0~
8.5, dissolved oxygen is less than 0.2mg/L.Anammox strain activity recovering device water inlet substrate concentration is ammonia nitrogen 5mmol/L, sub-
Nitrate nitrogen 5mmol/L, using the simulated wastewater manually prepared, composition includes nutrient salt solution, inorganic salt solution and originally
Water；It needs that nutrient salt solution 5mL and inorganic salt solution 5ml is added in every liter of water distribution without matrix；Nutrient salt solution composition are as follows:
NaH2PO43~5g/L, CaCl225~30g/L；Inorganic salt solution composition: FeSO4.7H21~2g/L of O, (NH4)6Mo7O24·
4H20.03~0.05g/L of O, CuSO4·5H20.04~0.06g/L of O, ZnSO4·6H20.05~0.10g/L of O, MnSO4
0.15~0.25g/L, CoCl2·6H20.04~0.06g/L of O, 3~5g/L of EDTA；Base is free of by above-mentioned requirements are prepared
The water distribution of matter is filled with high-purity argon gas 10~30 minutes using micropore aeration pipe, and measurement oxygen in water concentration is less than 0.2mg/L
Stop applying argon gas afterwards to get the anaerobic water without matrix is arrived.The hydraulic detention time of reactor is for 24 hours that effluent recycling ratio is
50%~100%.The water inlet substrate concentration of Anammox strain activity recovering device is gradually increased, with 3~5 days for a rank
Section is carried out according to the speed that per stage improves ammonia nitrogen 2mmol/L and nitrite nitrogen 2mmol/L.When substrate concentration reaches ammonia nitrogen
When 15mmol/L and nitrite nitrogen 15mmol/L, stop improving substrate concentration, and is gradually increased inflow, shortens hydraulic retention
Time gradually increases the operating load of Anammox strain activity recovering device.Hydraulic detention time according to for 24 hours, 18h,
The ladder of 12h, 8h, 4h are shortened, and every ladder maintains operation 3~5 days.
The water inlet total nitrogen volumetric loading of 3.6 Anammox strain activity recovering devices reaches 2.0~2.5kgTN/m3D,
Nitrogen removal rate reaches 80%, and total nitrogen removal efficiency reaches 1.5~2.0kgTN/m3Anammox strain when d, after storage
Activity recovery work complete, can carry out actual industrial waste water processing operation.
4. the storage of industrialization anaerobic ammonia oxidizing bacteria of the present invention and the method utilized again, are suitable for middle isotactic
More than mould strain storage and recycling occasion.The storage of strain and activity recovery utilize process again, using batch
Method carries out, and the strain of every batch of storage is at least 1m3, being converted into strain dry mass is 20kgVSS, living through over recovery after storage
Property, guarantee that the strain dry mass utilized again is 16kgVSS or more.
The present invention matches out the bacterium for being appropriate for storing for a long time by the screening to Anammox strain progress suitability
Kind；During storage by controlling the environmental factors such as different temperature condition, substrate concentration condition and pressure, realize not
With the optimum choice for the storage requirement being applicable under the shelf life, so that Anammox strain is respectively in short-term and long-term storage
The optimization of activity recovery efficiency after depositing.The mode of the activity recovery of strain after storage is optimized simultaneously, makes strain
Activity can rapidly restore, meet inoculation, the service requirement of industrial treatment device.Experimental study shows the storage side
Method has good adaptability for preferred anaerobic ammonia oxidizing bacteria, and during storage in 1~120 day, activity preservation rate can reach
To 60%~80%, the strain after over recovery can be rapidly reached 2.0kgTN/m in the Operating ettectiveness of reactor3The total nitrogen of d
Removal efficiency.It is right during long-time starting of long-distance transportation, anaerobic ammonia oxidation process etc. to solve anaerobic ammonia oxidizing bacteria
In the demand of high activity strain.
The invention has the benefit that can be according to the difference in the time limit of quasi- storage, targetedly selection is suitable for being store
Anammox strain is deposited, and by controlling the environmental conditions such as temperature, substrate concentration and pressure in storage process, to realize
The high activity of strain is stored.At short 1~30 day, the activity preservation rate that anaerobic ammonia oxidizing bacteria may be implemented in this method was
81.82%；At long-term storage 31~120 days, the activity preservation rate that anaerobic ammonia oxidizing bacteria may be implemented is 57.10%~
63.05%.By different maturity periods storage method, long-distance transportation, the Anammox that anaerobic ammonia oxidizing bacteria may be implemented be anti-
The demand processes such as restarting and storing for short-term high activity after answering device intermitten service, can also realize anaerobic ammonia oxidation reactor
The long period of initial start-up about 3~4 is below the moon for the demand of medium-term and long-term strain storage.Temperature of the present invention, base
The operations factors such as matter, microelement, argon gas inflation are common process control condition, and the operation in industrial applications is more simple
Just, low-cost, there is very high application value.
Detailed description of the invention
Fig. 1 shows the schematic diagrames of Anammox strain hold-up vessel
Fig. 2 indicates Anammox strain activity recovering device schematic diagram
Specific embodiment
Embodiment 1:
Anammox strain hold-up vessel in the present embodiment is as shown in Figure 1.Strain hold-up vessel 1 is that cylindrical type is stainless
Cylinder of steel, dischargeable capacity 1000L.The top of strain hold-up vessel 1 is provided with flange feed opening 2, bottom is provided with flange discharge port
4.The tank body of strain hold-up vessel 1 is provided with sheet frame visor 3, can observe liquid level and mud position in tank.Bottom in strain hold-up vessel 1
Portion is provided with ring-like micropore aeration pipe 5, is connected by admission line 6 with high-purity argon gas gas source 9, is arranged on admission line 6
Air inlet ball valve 7 and gas heating device 8.The middle part of strain hold-up vessel 1 is provided with drainpipe 12, and drainage ball valve is arranged thereon
13.Vacuum-pumping system, including negative pressure surge tank 14, vacuum meter 16, vacuum pump 18 and draining are provided on drainpipe 12
Valve 15 and drain tap 17.Air in negative pressure surge tank 14 is taken out by vacuum pump 18, negative pressure is generated, in order in drainpipe 12
Middle formation siphon drainage.The top of strain hold-up vessel 1 is provided with pressure gauge 19, and thermometer 20 characterizes the work shape in container
State.The top of strain hold-up vessel 1 is also provided with safety valve 10 and exhaust valve 11, guarantees that the pressure in strain hold-up vessel 1 exists
Within the scope of setting.
Anammox strain activity recovering device in the present embodiment is as shown in Fig. 2, Anammox strain activity is extensive
Apparatus for coating 21 is cylindrical type stainless steel hermetically sealed can, dischargeable capacity 2000L.It is heating and insulating layer 23 outside tank body, setting sheet frame is seen
Examine mirror 33, liquidometer 25 and valve of tank gauge 24.Mechanical stirring paddle 22 is set in tank body, is driven by frequency conversion stirring motor 30.Instead
Answer the mating setting feeding port 29 of device, water inlet 31, discharge outlet 27, argon gas import 34, exhaust outlet 32, discharge port 35 and access hole
28.Anammox strain activity recovering device 21 is run by sequencing batch reactor mode.
Specific step is as follows for the present embodiment:
(1) it will need to take out in the good Anammox fluidized-bed reactor of anaerobic ammonia oxidizing bacteria self-operating stored.
The anaerobic ammonium oxidation sludge that autoreactor upper, middle, and lower part is taken out respectively is respectively placed in natural sedimentation 30 in transfer container
Minute, using the supernatant in siphonage discharge container.Again by these three anaerobic ammonium oxidation sludges according to 2: 5: 3 volume fraction
It is added separately in Anammox strain hold-up vessel 1.
(2) anaerobic water for being free of matrix is prepared, consisting of: nutrient salt solution, inorganic salt solution and tap water；Every liter is not
It needs that nutrient salt solution 5mL and inorganic salt solution 5ml is added in water distribution containing matrix；Nutrient salt solution composition are as follows: NaH2PO43~
5g/L、CaCl225~30g/L；Inorganic salt solution composition: FeSO4.7H21~2g/L of O, (NH4)6Mo7O24·4H2O 0.03~
0.05g/L、CuSO4·5H20.04~0.06g/L of O, ZnSO4·6H20.05~0.10g/L of O, MnSO40.15~0.25g/
L、CoCl2·6H20.04~0.06g/L of O, 3~5g/L of EDTA；By the prepared water distribution without matrix of above-mentioned requirements, use
Micropore aeration pipe is filled with high-purity argon gas 10~30 minutes, and measurement oxygen in water concentration stops applying argon gas after being less than 0.2mg/L,
Obtain the anaerobic water without matrix.
(3) Anammox strain will be obtained in step (1), with the anaerobic water without matrix in step (2) in strain
It is eluriated in hold-up vessel 1, carries out natural sedimentation in 30 minutes later, strain hold-up vessel is discharged from drainpipe 12 using siphonage
Supernatant in 1.Specific water discharge method are as follows: first close drainage ball valve 13 and water discharging valve 15, open drain tap 17, open true
Sky pump 18 closes vacuum pump 18 and drain tap 17, later again successively when the negative pressure value of vacuum meter 16 reaches 20~30KPa
Drainage ball valve 13 and water discharging valve 15 are opened, makes drainage pipeline start to exclude supernatant in strain hold-up vessel 1, and pass through adjusting
Water discharging valve 15 controls the speed of draining.
After the supernatant in strain hold-up vessel 1 excludes, the anaerobic water without matrix is added from feed opening 2 and is washed in a pan
It washes.This elutriation process carries out 3 times altogether, to remove the matrix and other substances of Anammox strain itself institute band.
(4) anaerobic water without matrix of step (2) is added in strain hold-up vessel 1 to 1 volume of strain hold-up vessel
80%, according to the time limit of quasi- storage select be added strain hold-up vessel 1 in matrix amount.When the shelf life was at 1~30 day
Short when, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should make the matrix in strain hold-up vessel 1 dense
Degree respectively reaches ammonia nitrogen 5mmol/L, nitrite nitrogen 5mmol/L.The air inlet ball valve 7 for opening admission line 6, passes through high-purity argon gas
Micropore aeration pipe 5 is uniformly filled into the mixed liquor in strain hold-up vessel 1, is inflated 10 minutes, guarantees strain hold-up vessel
Interior dissolved oxygen concentration is lower than 0.2mg/L.Later, into strain hold-up vessel 1, addition is free of base while being filled with high-purity argon gas
The anaerobic water of matter covers the flange seal lid of feed inlet 2 to the 90% of strain hold-up vessel total measurement (volume) later.
(5) air inlet ball valve 7 is opened, high-purity argon gas is filled in the hold-up vessel 1 being sealed in step (4), is controlled in bottle
Pressure rise speed pressure in 0.01MPa~0.02MP/min, final strain hold-up vessel 1 reaches 0.2~0.25MPa.It closes
Air inlet ball valve 7 is closed, and checks the sealing situation of strain hold-up vessel 1.
(6) storage temperature is selected according to the time limit of quasi- storage.Carrying out short-term anaerobic ammonia oxidizing bacteria storage in 1~30 day
When, the temperature condition that uses is 20 ± 4 DEG C of room temperature environment.
(7) activity recovery of the Anammox strain after storing: the pressure in strain hold-up vessel 1 is slowly decompressed to
Normal pressure controls decompression rate 0.005MPa~0.01MPa/min.Strain hold-up vessel 1 after decompression is passed through high-purity argon gas progress
Stirring heating opens heat riser 8 and provides heat for high-purity argon gas.Heating rate is 1~2 DEG C/h, finally controls temperature
30 DEG C or so, stand 4~6h.Strain after heating uses the anaerobic water without matrix to be eluriated, and argon gas stirs 10min, quiet
After setting 1~2h, supernatant is excluded using siphonage.It eluriates and carries out 2 times altogether, to remove the residual matrix on strain surface, metabolism produces
Object and the dead thallus to disintegrate.It can be taken off active Anammox to be restored from the discharge port of 1 bottom of strain hold-up vessel
Strain.
(8) that active Anammox strain to be restored is put into Anammox strain activity as shown in Figure 2 is extensive
Active fast quick-recovery is carried out in apparatus for coating 21.Anammox strain activity recovering device 21 is added from batching hole 29 in strain
Interior, the simulated wastewater manually prepared is added from water inlet 31, and Liquid level is in 80% position of tank body effective height.Anaerobism ammonia
Oxidation strain activity recovering device 21 is run by batch-type mode, living in Anammox strain to increase strain to greatest extent
Persistence in property recovery device 21.In operational process close feeding port 29, water inlet 31, open exhaust outlet 32, from argon gas into
Mouth 34 is passed through high-purity argon gas 20~30 minutes, to guarantee the anaerobic state in reactor, while opening mechanical stirring paddle 22.Reaction
After the time for reaching setting, stop mechanical stirring paddle 22 and keep quiet heavy 1h, supernatant is discharged from discharge outlet 27, completes a reaction
Period.The operation of next cycle can then be carried out.
The main control condition of Anammox strain activity recovering device during activity recovery are as follows: temperature 32~35
DEG C, pH=7.0~8.5, dissolved oxygen is less than 0.2mg/L.Reactor water inlet substrate concentration is ammonia nitrogen 5mmol/L, nitrite nitrogen
5mmol/L, using the simulated wastewater manually prepared, (nutritional ingredient therein and microelement are shown in that strain stores the step in trifle
2).The hydraulic detention time for controlling reactor first is that for 24 hours, effluent recycling ratio is 50%~100%, and anaerobism ammonia oxygen is gradually increased
Change bacterial activity recovery device water inlet substrate concentration, with 3 days be a stage, according to per stage improve ammonia nitrogen 2mmol/L and
The speed of nitrite nitrogen 2mmol/L carries out.When substrate concentration reaches ammonia nitrogen 15mmol/L and nitrite nitrogen 15mmol/L,
Stop improving substrate concentration.Hydraulic detention time then is gradually shortened, gradually increases the operating load of reactor.When hydraulic retention
Between according to for 24 hours, the ladder of 18h, 12h, 8h, 4h shortened, every ladder maintains operation 3 days.
The water inlet total nitrogen volumetric loading of Anammox strain activity recovering device reaches 2.0~2.5kgTN/m3D, total nitrogen
Removal rate reaches 80%, and total nitrogen removal efficiency reaches 1.5~2.0kgTN/m3When d, Anammox strain after storage it is extensive
It brings back to life sex work to complete, the processing operation of actual industrial waste water can be carried out.
The Anammox specific activity of 4 parts of Anammox strain storage front and backs is measured respectively in the present embodiment,
Obtain the anaerobic ammoxidation activity retention rate of every part of sample.Being averaged for 30 days strains is stored under 20 ± 4 DEG C of room temperature environment
Activity preservation rate is 81.82%, and putting into anaerobic ammonia oxidation reactor can rapid activity recovery.
Embodiment 2:
Anammox strain hold-up vessel in the present embodiment is as shown in Figure 1.Strain hold-up vessel 1 is cylindrical type pressure resistance
Glass fiber reinforced plastic tank, dischargeable capacity 1000L.The top of strain hold-up vessel 1 is provided with flange feed opening 2, bottom is provided with flange and unloads
Material mouth 4.The tank body of strain hold-up vessel 1 is provided with sheet frame visor 3, can observe liquid level and mud position in tank.Strain hold-up vessel 1
Interior bottom is provided with ring-like micropore aeration pipe 5, is connected by admission line 6 with high-purity argon gas gas source 9, on admission line 6
Air inlet ball valve 7 and gas heating device 8 are set.The middle part of strain hold-up vessel 1 is provided with drainpipe 12, and draining ball is arranged thereon
Valve 13.Vacuum-pumping system, including negative pressure surge tank 14, vacuum meter 16, vacuum pump 18 and row are provided on drainpipe 12
Penstock 15 and drain tap 17.Air in negative pressure surge tank 14 is taken out by vacuum pump 18, negative pressure is generated, in order in drainpipe
Siphon drainage is formed in 12.The top of strain hold-up vessel 1 is provided with pressure gauge 19, and thermometer 20 characterizes the work in container
State.The top of strain hold-up vessel 1 is also provided with safety valve 10 and exhaust valve 11, guarantees the pressure in strain hold-up vessel 1
Within the scope of setting.
Anammox strain activity recovering device in the present embodiment is as shown in Fig. 2, Anammox strain activity is extensive
Apparatus for coating 21 is cylindrical type stainless steel hermetically sealed can, dischargeable capacity 2000L.It is heating and insulating layer 23 outside tank body, setting sheet frame is seen
Examine mirror 33, liquidometer 25 and valve of tank gauge 24.Mechanical stirring paddle 22 is set in tank body, is driven by frequency conversion stirring motor 30.Instead
Answer the mating setting feeding port 29 of device, water inlet 31, discharge outlet 27, argon gas import 34, exhaust outlet 32, discharge port 35 and access hole
28.Reactor 21 is run by sequencing batch reactor mode.
Specific step is as follows for the present embodiment:
(1) it will need to take out in the good Anammox fluidized-bed reactor of anaerobic ammonia oxidizing bacteria self-operating stored.
The anaerobic ammonium oxidation sludge that autoreactor upper, middle, and lower part is taken out respectively is respectively placed in natural sedimentation 30 in transfer container
Minute, using the supernatant in siphonage discharge container.Again by these three anaerobic ammonium oxidation sludges according to 2: 5: 3 volume fraction
It is added separately in Anammox strain hold-up vessel 1.
(2) anaerobic water for being free of matrix is prepared, consisting of: nutrient salt solution, inorganic salt solution and tap water；Every liter is not
It needs that nutrient salt solution 5mL and inorganic salt solution 5ml is added in water distribution containing matrix；Nutrient salt solution composition are as follows: NaH2PO43~
5g/L、CaCl225~30g/L；Inorganic salt solution composition: FeSO4.7H21~2g/L of O, (NH4)6Mo7O24·4H2O 0.03~
0.05g/L、CuSO4·5H20.04~0.06g/L of O, ZnSO4·6H20.05~0.10g/L of O, MnSO40.15~0.25g/
L、CoCl2·6H20.04~0.06g/L of O, 3~5g/L of EDTA；By the prepared water distribution without matrix of above-mentioned requirements, use
Micropore aeration pipe is filled with high-purity argon gas 10~30 minutes, and measurement oxygen in water concentration stops applying argon gas after being less than 0.2mg/L,
Obtain the anaerobic water without matrix.
(3) Anammox strain will be obtained in step (1), with the anaerobic water without matrix in step (2) in strain
It is eluriated in hold-up vessel 1, carries out natural sedimentation in 30 minutes later, strain hold-up vessel is discharged from drainpipe 12 using siphonage
Supernatant in 1.Specific water discharge method are as follows: first close drainage ball valve 13 and water discharging valve 15, open drain tap 17, open true
Sky pump 18 closes vacuum pump 18 and drain tap 17, later again successively when the negative pressure value of vacuum meter 16 reaches 20~30KPa
Drainage ball valve 13 and water discharging valve 15 are opened, makes drainage pipeline start to exclude supernatant in strain hold-up vessel 1, and pass through adjusting
Water discharging valve 15 controls the speed of draining.
After the supernatant in strain hold-up vessel 1 excludes, the anaerobic water without matrix is added from feed opening 2 and is washed in a pan
It washes.This elutriation process carries out 3 times altogether, to remove the matrix and other substances of Anammox strain itself institute band.
(4) anaerobic water without matrix that step (2) are added in strain hold-up vessel 1 always holds to strain hold-up vessel 1
Long-pending 80%, according to the amount for the matrix that the time limit of quasi- storage selects to be added in strain hold-up vessel 1.Shelf life is 31~120
When it long-term storage, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should be such that the substrate concentration in container 1 distinguishes
Reach ammonia nitrogen 10mmol/L, nitrite nitrogen 10mmol/L.The air inlet ball valve 7 for opening admission line 6, makes high-purity argon gas through micropore
Aeration tube 5 is uniformly filled into the mixed liquor in strain hold-up vessel 1, is inflated 10 minutes, is guaranteed molten in strain hold-up vessel
It solves oxygen concentration and is lower than 0.2mg/L.Later, into strain hold-up vessel 1, addition is free of matrix while being filled with high-purity argon gas
Anaerobic water covers the flange seal lid of feed inlet 2 to the 90% of strain hold-up vessel total measurement (volume) later.
(5) air inlet ball valve 7 is opened, high-purity argon gas is filled in the hold-up vessel 1 being sealed in step (4), is controlled in bottle
Pressure rise speed pressure in 0.01MPa~0.02MP/min, final strain hold-up vessel l reaches 0.2~0.25MPa.It closes
Air inlet ball valve 7 is closed, and checks the sealing situation of strain hold-up vessel 1.
(6) storage temperature is selected according to the time limit of quasi- storage, the long-term anaerobic ammonia oxidizing bacteria storage in carrying out 31~120 days
When depositing, using thermostatic chamber or refrigerator, keeping temperature condition is 4 ± 1 DEG C.
(7) activity recovery of the Anammox strain after storing: the pressure in strain hold-up vessel 1 is slowly decompressed to
Normal pressure controls decompression rate 0.005MPa~0.01MPa/min.Strain hold-up vessel 1 after decompression is passed through high-purity argon gas progress
Stirring heating opens heat riser 8 and provides heat for high-purity argon gas.Temperature-rise period uses ladder-elevating temperature method, heating rate 1
~2 DEG C/h, it is warming up to 20 DEG C first, stop heating and stirs and 20 DEG C of conditions is maintained to stand 4~6h；Finally temperature is controlled
30 DEG C or so, stand 4~6h.Strain after heating uses the anaerobic water without matrix to be eluriated, and argon gas stirs 10min, quiet
After setting 1~2h, supernatant is excluded using siphonage.It eluriates and carries out 2 times altogether, to remove the residual matrix on strain surface, metabolism produces
Object and the dead thallus to disintegrate.It can be taken off active Anammox to be restored from the discharge port of 1 bottom of strain hold-up vessel
Strain.
(8) that active Anammox strain to be restored is put into Anammox strain activity as shown in Figure 2 is extensive
Active fast quick-recovery is carried out in apparatus for coating 21.Anammox strain activity recovering device 21 is added from batching hole 29 in strain
Interior, the simulated wastewater manually prepared is added from water inlet 31, and Liquid level is in 80% position of tank body effective height.Anaerobism ammonia
Oxidation strain activity recovering device 21 is run by batch-type mode, living in Anammox strain to increase strain to greatest extent
Withholding in property recovery device 21.In operational process close feeding port 29, water inlet 31, open exhaust outlet 32, from argon gas into
Mouth 34 is passed through high-purity argon gas 20~30 minutes, to guarantee the anaerobic state in reactor, while opening mechanical stirring paddle 22.Reaction
After the time for reaching setting, stop mechanical stirring paddle 22 and keep quiet heavy 1h, supernatant is discharged from discharge outlet 27, completes a reaction
Period.The operation of next cycle can then be carried out.
The main control condition of Anammox strain activity recovering device during activity recovery are as follows: temperature 32~35
DEG C, pH=7.0~8.5, dissolved oxygen is less than 0.2mg/L.Reactor water inlet substrate concentration is ammonia nitrogen 5mmol/L, nitrite nitrogen
5mmol/L, using the simulated wastewater manually prepared, (nutritional ingredient therein and microelement are shown in that strain stores the step in trifle
2).The hydraulic detention time for controlling reactor first is that for 24 hours, effluent recycling ratio is 50%~100%, and anaerobism ammonia oxygen is gradually increased
Change bacterial activity recovery device water inlet substrate concentration, with 5 days be a stage, according to per stage improve ammonia nitrogen 2mmol/L and
The speed of nitrite nitrogen 2mmol/L carries out.When substrate concentration reaches ammonia nitrogen 15mmol/L and nitrite nitrogen 15mmol/L,
Stop improving substrate concentration.Hydraulic detention time then is gradually shortened, gradually increases the operating load of reactor.When hydraulic retention
Between according to for 24 hours, the ladder of 18h, 12h, 8h, 4h shortened, every ladder maintains operation 5 days.
The water inlet total nitrogen volumetric loading of Anammox strain activity recovery reaction reaches 2.0~2.5kgTN/m3D, total nitrogen
Removal rate reaches 80%, and total nitrogen removal efficiency reaches 1.5~2.0kgTN/m3When d, Anammox strain after storage it is extensive
Sex work is brought back to life to complete, the Anammox strain of the state can be directly inoculated into the processing unit of the industrial wastewater containing ammonia,
Denitrification effect is good.
The Anammox specific activity of 4 parts of Anammox strain storage front and backs is measured respectively in the present embodiment,
Obtain the anaerobic ammoxidation activity retention rate of every part of sample.The activity that 31~120 days strains are stored in 4 ± 1 DEG C of environment is protected
Staying rate is 57.10~63.05%.The activity recovery time put into anaerobic ammoxidation activity recovery device about needs 20 days.
Claims (4)
1. a kind of storage of industrialization anaerobic ammonia oxidizing bacteria and the method utilized again, operating procedure are as follows:
Step 1: the selection of Anammox strain
From the Anammox fluidized-bed reactor of normal operation or the upper, middle and lower three of up flow anaerobic sludge blanket reactor
Divide and take out anaerobic ammonium oxidation sludge respectively, uniformly mixed according to 2: 5: 3 volumetric ratio, high-purity argon gas is used in mixed process
The mode of perforated pipe aerating regulation is stirred, the maximum anaerobic ammoxidation activity of the mixing anaerobic ammoxidation strain measured, most
Big Anammox strain of the anaerobic ammoxidation activity greater than 0.15kgTN/kgVSS.d is suitable for being stored；
Step 2: the storage of Anammox strain
The Anammox strain for meeting shelf appeal that step 1 is obtained, is placed in Anammox strain hold-up vessel, uses
Anaerobic water without matrix is eluriated 3 times, is eluriated volume of the clean Anammox strain in strain hold-up vessel and is accounted for strain
The 40%~50% of hold-up vessel volume；The anaerobic water without matrix is filled in strain hold-up vessel to the 80% of total measurement (volume)；
Depot matrix is added, the substrate concentration in strain hold-up vessel is made to respectively reach ammonia nitrogen 5mmol~10mmol/L, nitrite nitrogen
5mmol~10mmol/L, the anaerobic state being filled in high-purity argon gas holding container, guarantees dissolved oxygen concentration in strain hold-up vessel
Lower than 0.2mg/L；Later, while being filled with high-purity argon gas into strain hold-up vessel anaerobic water of the addition without matrix to total
The 90% of volume, covers the sealing of flange seal lid, and the pressure in strain hold-up vessel is controlled in 0.2~0.25MPa, high-purity argon gas
Be filled with balance slowly, control pressure rise speed in 0.01MPa~0.02MP/min；Air intake valve is closed later, and is checked
Strain hold-up vessel seals situation, it is ensured that strain hold-up vessel sealing performance is intact；Temperature condition is controlled 4 ± 1 DEG C~20 ± 4
Between DEG C；During storage, strain hold-up vessel is in light protected environment, and the sealing feelings in strain hold-up vessel are checked daily
Condition guarantees the pressure in the bottle between 0.2MPa~0.25MPa by being filled with a small amount of argon gas or opening relief valve；Described is free of
The anaerobic water of matrix, composition include nutrient salt solution, inorganic salt solution and tap water；It needs to add in every liter of water distribution without matrix
Enter nutrient salt solution 5mL and inorganic salt solution 5ml；Nutrient salt solution composition are as follows: NaH2PO43~5g/L, CaCl225~30g/L；
Inorganic salt solution composition: FeSO4.7H21~2g/L of O, (NH4)6Mo7O24·4H20.03~0.05g/L of O, CuSO4·5H2O
0.04~0.06g/L, ZnSO4·6H20.05~0.10g/L of O, MnSO40.15~0.25g/L, CoCl2·6H2O 0.04~
3~5g/L of 0.06g/L, EDTA；By the prepared water distribution without matrix of above-mentioned requirements, it is filled with using micropore aeration pipe high-purity
Argon gas 10~30 minutes, measurement oxygen in water concentration be less than 0.2mg/L after stop applying argon gas to get to without matrix nothing
Oxygen water；The depot matrix is the ammonium bicarbonate soln of 1.0g/L and the sodium nitrite solution of 1.0g/L, respectively to anaerobism ammonia
It aoxidizes in strain storage liquid and ammonia nitrogen and nitrite nitrogen is provided；
The activation recovering of Anammox strain after step 3, storage
The quasi- Anammox strain hold-up vessel for carrying out strain and utilizing again, by the pressure of 0.005MPa~0.01MPa/min
It reduces speed to carry out being decompressed to normal pressure, then is passed through the high-purity argon gas of controllable temperature and is stirred heating, the speed of heating is 1~2
DEG C/h, the Anammox strain of storage is warming up to 30 DEG C, stands 4~6h；Strain after heating uses the nothing without matrix
Oxygen water is eluriated, and argon gas stirs 10min, after standing 1~2h, excludes supernatant using siphonage；It eluriates and carries out 2 times altogether, with
Remove the residual matrix, metabolite and the dead thallus to disintegrate on strain surface；Anammox strain after elutriation can be thrown
Enter in anaerobic ammoxidation activity recovery device；The main control condition of anaerobic ammoxidation activity recovery device are as follows: temperature 32~35
DEG C, pH=7.0~8.5, dissolved oxygen is less than 0.2mg/L；Reactor water inlet substrate concentration is ammonia nitrogen 5mmol/L, nitrite nitrogen
5mmol/L, using the simulated wastewater manually prepared, the hydraulic detention time of reactor is for 24 hours that effluent recycling ratio is 50%~
100%；The water inlet substrate concentration of reactor is gradually increased, with 3~5 days for a stage, improves ammonia nitrogen according to per stage
The speed of 2mmol/L and nitrite nitrogen 2mmol/L carry out；When substrate concentration reaches ammonia nitrogen 15mmol/L and nitrite nitrogen
When 15mmol/L, stop improving substrate concentration, and be gradually increased inflow, shorten hydraulic detention time, gradually increases reactor
Operating load；Hydraulic detention time according to for 24 hours, the ladder of 18h, 12h, 8h, 4h shortened, every ladder maintains operation 3~5
It；The water inlet total nitrogen volumetric loading for carrying out the anaerobic ammonia oxidation reactor of activation recovering reaches 2.0~2.5kgTN/m3D, total nitrogen
Removal rate reaches 80%, and total nitrogen removal efficiency reaches 1.5~2.0kgTN/m3When d, the work of the Anammox strain after storage
Property is resumed work completion.
2. a kind of storage of industrialization anaerobic ammonia oxidizing bacteria and the method utilized again, feature exist according to claim 1
When short 1~30 day, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should make the matrix in hold-up vessel
Concentration respectively reaches ammonia nitrogen 5mmol/L, nitrite nitrogen 5mmol/L, and the temperature condition used is 20 ± 4 DEG C of room temperature environment.
3. a kind of storage of industrialization anaerobic ammonia oxidizing bacteria and the method utilized again, feature exist according to claim 1
When long-term storage 31~120 days, the ammonium bicarbonate soln of addition and the amount of sodium nitrite solution should make the base in hold-up vessel
Matter concentration respectively reaches ammonia nitrogen 10mmol/L, nitrite nitrogen 10mmol/L, and keeping temperature condition is 4 ± 1 DEG C.
4. a kind of storage of industrialization anaerobic ammonia oxidizing bacteria and the method utilized again, feature exist according to claim 1
When anaerobism ammonia nitriding bacterium carries out industrialization storage, the strain of every batch of storage is at least 1m3, being converted into dry weight is 20kgVSS；
By activation recovering after storage, it is ensured that the strain dry weight utilized again is 16kgVSS or more.
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