CN106830302B - MBR (membrane bioreactor) whole course nitrobacteria enrichment device and method for automatically controlling dissolved oxygen - Google Patents
MBR (membrane bioreactor) whole course nitrobacteria enrichment device and method for automatically controlling dissolved oxygen Download PDFInfo
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- CN106830302B CN106830302B CN201710041783.1A CN201710041783A CN106830302B CN 106830302 B CN106830302 B CN 106830302B CN 201710041783 A CN201710041783 A CN 201710041783A CN 106830302 B CN106830302 B CN 106830302B
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Abstract
The invention discloses an MBR (membrane bioreactor) whole course nitrobacteria enrichment device and method for automatically controlling dissolved oxygen. The reactor comprises a reactor, a water inlet barrel, a water bath kettle and a control cabinet, wherein the main body of the reactor is a cylindrical tank body, a water inlet is arranged below the tank body, water is discharged through a built-in membrane component, filling materials are filled in the tank body, a DO probe and a sampling port are arranged above the tank body, a temperature control jacket is arranged on the outer side of the tank body, and an aeration disc is arranged at the bottom of the tank body, so that the concentration of dissolved oxygen in the reactor can be regulated and controlled, meanwhile, the stirring effect can be achieved, and the membrane component is prevented from being blocked. The invention can provide an environment with low dissolved oxygen and low nutrient substances for the whole course nitrobacteria, the specific surface area of the filler filled in the reactor is large, the filler can be attached to and grow by microorganisms, the effluent adopts a membrane component, the loss of biomass can be effectively prevented, and the dissolved oxygen in the reactor is controlled at a lower level by a dissolved oxygen probe, an air pump and a nitrogen steel cylinder, so that the effective enrichment of the whole course nitrobacteria is realized, and a high-purity enriched culture is obtained.
Description
Technical Field
The invention belongs to the field of environment-friendly devices, and particularly relates to an MBR (membrane bioreactor) whole course nitrobacteria enrichment device and method for automatically controlling dissolved oxygen.
Background
Nitrification has long been known to occur in two steps, the first step being the removal of ammonia (NH) from ammonia-oxidizing bacteria (AOB) or ammonia-oxidizing archaea (AOA) 4 + ) By oxidation to Nitrite (NO) 2 - ) The second step is the Nitrite (NO) oxidation by Nitrite Oxidizing Bacteria (NOB) 2 - ) By oxidation to Nitrate (NO) 3 - ) Neither ammonia-oxidizing microorganisms nor nitrite-oxidizing bacteria (NOB) are capable of oxidizing both species simultaneously. However, energy analysis shows that the whole course nitration can obtain larger energy than a single step oxidation reaction, and theoretically, the whole course nitration bacteria exist. The Costa group studied the mechanisms by which nitrification was carried out in steps over a decade ago and it was thought that dividing nitrification into two steps could increase growth rate, but the microorganisms gained less energy from each mole of substrate. While one-step total nitrification can obtain more energy per mole of substrate, the growth rate is slower due to the long reaction path. Thus, the whole-course nitrifying bacteria tend to grow in slow-flowing, low-concentration habitat growth substrates and tend to form microbial flocs or biofilms. In 2015, Holger Daims, Maartje a.h.j.van Kessel et al, successively discovered and cultured strains capable of simultaneously oxidizing ammonia to nitrite and nitrite to nitrate, all belonging to the genus Nitrospira.
From the first discovery of the whole course nitrobacteria to the present time of more than one year, the research on the whole course nitrobacteria is still in a starting stage, and a large number of puzzles are waited for the uncovering of people, so the research on the physiological and biochemical characteristics of the whole course nitrobacteria has important significance. However, the slow growth of the whole course nitrifying bacteria and the competition of the enrichment culture from ammonia oxidizing bacteria, ammonia oxidizing archaea, nitrite oxidizing bacteria and anaerobic ammonia oxidizing bacteria lead to the difficulty in obtaining high-purity whole course nitrifying bacteria enrichment culture, which greatly limits the mechanism research and application research of the reaction. In order to obtain a high-purity enrichment culture of the whole course nitrifying bacteria, a device with selective enrichment conditions is needed to be provided, namely the growth of the whole course nitrifying bacteria is selectively promoted, and the growth of other bacteria is limited, so that a device specially used for the enrichment of the whole course nitrifying bacteria is needed to be designed.
Disclosure of Invention
The invention aims to overcome the defect that the biomass of the conventional device is easy to lose by adding a membrane module into a reactor, provide an environment with low dissolved oxygen and low substrate concentration for enrichment culture of whole course nitrobacteria and construct an MBR whole course nitrobacteria enrichment device with automatic dissolved oxygen control.
The MBR whole-course nitrobacteria enrichment device automatically controlled by dissolved oxygen comprises a reactor, a water bath and a water inlet barrel, wherein the reactor comprises a cylindrical tank body, a reactor water inlet, a built-in membrane component, a vacuum meter, a self-priming pump, a regulating valve, a flowmeter, an aeration disc, a DO probe, a reactor sampling port, a temperature control jacket water outlet, a temperature control jacket water inlet, an air valve, an electromagnetic valve, a nitrogen steel cylinder, a water inlet barrel feeding pipe, a water inlet pump and an air pump; the reactor main body is a cylindrical tank body, a reactor water inlet is arranged on the lower side of the tank body, a built-in membrane assembly is arranged in the tank body and used for discharging water out of the reactor, the membrane assembly is sequentially connected with a vacuum meter, a self-priming pump, a regulating valve and a flowmeter and used for regulating and controlling the water discharging speed, an aeration disc is arranged on the lower side of the membrane assembly, an air inlet of the aeration disc is divided into two paths and respectively connected with a nitrogen steel cylinder and an air pump, an electromagnetic valve is arranged on a pipeline connected with the nitrogen steel cylinder, and an air valve is arranged on a pipeline connected with the air pump; the top of the reactor is provided with a DO probe and a reactor sampling port, the outer side of the reactor is provided with a hollow temperature control jacket, the lower part of the temperature control jacket is provided with a temperature control jacket water inlet communicated with the inner cavity, and the upper part of the temperature control jacket water outlet communicated with the inner cavity; the water bath kettle is connected with the water outlet of the temperature control jacket and the water inlet of the temperature control jacket to form a circulation loop; the water inlet of the reactor is connected with a water inlet barrel feed pipe through a water inlet pump, and the water inlet barrel feed pipe is arranged in the water inlet barrel.
Preferably, a control cabinet is further arranged and is connected with the DO probe, the air valve and the electromagnetic valve.
Preferably, the reactor is filled with a filler.
The invention also aims to provide a whole course nitrobacteria enrichment method by utilizing the MBR device, which comprises the following steps: inoculating 10-20% of full-course nitrifying bacteria seed mud in a reactor, injecting a low-concentration culture medium into the reactor, and culturing and enriching microorganisms to ensure that the full-course nitrifying bacteria are attached to the surface of a filler to form a biological film; in the enrichment process, a continuous water inlet and continuous water outlet mode is adopted, and microorganisms are completely intercepted in the bioreactor through a built-in membrane module during water outlet; meanwhile, detecting the dissolved oxygen of the culture medium in the reactor through a DO probe, and regulating the air quantity and the nitrogen quantity through regulating and controlling an air valve and an electromagnetic valve to keep the dissolved oxygen in the reactor at a level lower than 1 mg/L; the temperature of the culture medium is controlled to be 25-30 ℃.
Preferably, the aeration disc is used for carrying out intermittent aeration, so that the effects of oxygen control and stirring are achieved, and the membrane module is prevented from being blocked.
Compared with the prior art, the invention has the innovation that: 1) the enrichment condition of the whole course nitrobacteria is special, the invention provides proper conditions of low dissolved oxygen, low nutrient concentration, medium temperature and the like for the nitrobacteria, and the change of the dissolved oxygen in the reactor is monitored in real time by a dissolved oxygen probe; 2) the reaction tank is MBR, and the built-in membrane module is adopted for water outlet, so that microorganisms are completely trapped in the bioreactor, thereby effectively avoiding the loss of biomass and being beneficial to the growth and enrichment of nitrifying bacteria in the whole process; 3) the filler is filled in the tank body, so that the attachment of microorganisms and the generation of a biological film are facilitated, and an environment favorable for the growth of whole-course nitrifying bacteria is created; 4) the aeration disc can not only adjust the dissolved oxygen content in the reactor, but also play a role in stirring and preventing the membrane module from being blocked.
Drawings
FIG. 1 is a schematic structural diagram of an MBR whole course nitrifying bacteria enrichment device with automatic dissolved oxygen control.
In the figure: the device comprises a reactor 1, a water bath 2, a control cabinet 3 and a water inlet barrel 4, wherein the reactor 1 comprises a cylindrical tank body 5, a reactor water inlet 6, a built-in membrane component 7, a vacuum meter 8, a self-priming pump 9, a regulating valve 10, a flowmeter 11, an aeration disc 12, a filler 13, a DO probe 14, a reactor sampling port 15, a temperature control jacket 16, a temperature control jacket water outlet 17, a temperature control jacket water inlet 18, an air valve 19, an electromagnetic valve 20, a nitrogen steel cylinder 21, a water inlet barrel feeding pipe 22, a water inlet pump 23 and an air pump 24.
Detailed Description
As shown in fig. 1, the MBR whole-course nitrobacter enrichment device for automatically controlling dissolved oxygen comprises a reactor 1, a water bath 2, a control cabinet 3 and a water inlet barrel 4, wherein the reactor 1 comprises a cylindrical tank body 5, a reactor water inlet 6, a built-in membrane module 7, a vacuum meter 8, a self-priming pump 9, an adjusting valve 10, a flow meter 11, an aeration disc 12, a filler 13, a DO probe 14, a reactor sampling port 15, a temperature control jacket 16, a temperature control jacket water outlet 17, a temperature control jacket water inlet 18, an air valve 19, an electromagnetic valve 20, a nitrogen steel cylinder 21, a water inlet barrel feeding pipe 22, a water inlet pump 23 and an air pump 24; the reactor 1 is mainly a cylindrical tank 5, a reactor water inlet 6 is arranged on the lower side of the tank, a built-in membrane module 7 is arranged in the tank and used for discharging water out of the reactor, the membrane module is sequentially connected with a vacuum meter 8, a self-sucking pump 9, a regulating valve 10 and a flowmeter 11 and used for regulating and controlling the water discharging speed, an aeration disc 12 is arranged on the lower side of the membrane module, an air inlet of the aeration disc is divided into two paths and respectively connected with a nitrogen steel cylinder 21 and an air pump 24, an electromagnetic valve 20 is arranged on a pipeline connected with the nitrogen steel cylinder 21, and an air valve 19 is arranged on a pipeline connected with the air pump 24; the top of the reactor is provided with a DO probe 14 and a reactor sampling port 15, the outer side of the reactor is provided with a hollow temperature control jacket 16, the lower part of the temperature control jacket is provided with a temperature control jacket water inlet 18 communicated with the inner cavity, and the upper part of the temperature control jacket water outlet 17 communicated with the inner cavity; the water bath 2 is connected with a water outlet 17 of the temperature control jacket and a water inlet 18 of the temperature control jacket to form a circulation loop; the water inlet 6 of the reactor is connected with a water inlet barrel feeding pipe 22 through a water inlet pump 23, and the water inlet barrel feeding pipe 22 is arranged in the water inlet barrel 4. The control cabinet 3 is connected to the DO probe 14, the air valve 19 and the solenoid valve 20. The reactor 1 is filled with a filler 13 as a carrier to which nitrobacteria are attached.
In the invention, the reactor 1 adopts the built-in membrane module 7 to discharge water, so that microorganisms are completely trapped in the bioreactor, thereby effectively avoiding the loss of biomass and being beneficial to the growth and enrichment of nitrifying bacteria in the whole process. The reactor 1 is filled with the filler 13, the filler has large specific surface area and good hydrophilicity, is beneficial to the attachment of microorganisms and the generation of a biological film, and provides a proper growth environment for the whole course of nitrobacteria. The bottom of the reactor 1 is provided with an aeration disc 12 which is connected with an air valve 19 and an electromagnetic valve 20, and the control cabinet 3 can adjust the air inlet ratio and the air inlet amount of air and nitrogen so as to regulate and control the dissolved oxygen concentration in the reactor, and the intermittent aeration can play a role in stirring and prevent the membrane component from being blocked. The temperature of the reactor 1 is controlled by a water bath 2 and a temperature control jacket 1, and is controlled to be 25-30 ℃; the temperature control jacket 16 wraps the middle part and the bottom part of the cylindrical tank body 5, and the interval (namely the thickness of the inner cavity) between the temperature control jacket and the cylindrical tank body 5 is 1-2 cm.
The strain enrichment method of the MBR whole course nitrifying bacteria enrichment device with automatic dissolved oxygen control comprises the following steps: 10-20 percent (the sludge accounts for the effective volume of the reactor) of full-course nitrifying bacteria seed sludge is inoculated in the reactor 1, and microorganisms are attached to the filler to form a biological film after contacting the filler 13. A culture medium with low ammonia nitrogen concentration (ammonia nitrogen is less than or equal to 0.5mM) in the water inlet barrel 4 enters the cylindrical tank body 5 through the inlet pipe 22 and the reactor water inlet 6 under the action of the water inlet pump 23, the water outlet adopts the built-in membrane component 7, negative pressure is formed in the membrane under the action of the self-sucking pump 9, the water in the reactor is filtered and then is pumped out, and the water outlet flow can be adjusted according to requirements. The bottom of the reactor is provided with an aeration disc 12 which is connected with an air valve 19 and an electromagnetic valve 20, the control cabinet 3 detects the dissolved oxygen in the reactor through a DO probe 14, and adjusts the air amount and the nitrogen amount through regulating and controlling the air valve 19 and the electromagnetic valve 20, so that the dissolved oxygen in the reactor 1 is maintained at a lower level (below 1 mg/L). Besides controlling the concentration of dissolved oxygen, aeration also plays a role in stirring, and effectively solves the problem that the built-in membrane module 7 is easy to block; the water bath 2 is connected with a water inlet 18 and a water outlet 17 of a temperature control jacket 16, the temperature of the reactor is adjusted, and the temperature of the culture medium is controlled to be 25-30 ℃.
Claims (4)
1. An MBR whole-course nitrobacteria enrichment device capable of automatically controlling dissolved oxygen is characterized by comprising a reactor (1), a water bath (2) and a water inlet barrel (4), wherein the reactor (1) comprises a cylindrical tank body (5), a reactor water inlet (6), a built-in membrane module (7), a vacuum meter (8), a self-sucking pump (9), a regulating valve (10), a flow meter (11), an aeration disc (12), a DO probe (14), a reactor sampling port (15), a temperature control jacket (16), a temperature control jacket water outlet (17), a temperature control jacket water inlet (18), an air valve (19), an electromagnetic valve (20), a nitrogen steel cylinder (21), a water inlet barrel feeding pipe (22), a water inlet pump (23) and an air pump (24); the reactor (1) is mainly a cylindrical tank body (5), a reactor water inlet (6) is arranged on the lower side of the tank body, a built-in membrane assembly (7) is arranged in the tank body and used for discharging water from the reactor, the membrane assembly is sequentially connected with a vacuum meter (8), a self-priming pump (9), a regulating valve (10) and a flowmeter (11) and used for regulating and controlling the water outlet rate, an aeration disc (12) is arranged on the lower side of the membrane assembly, air inlets of the aeration disc are divided into two paths and respectively connected with a nitrogen steel cylinder (21) and an air pump (24), an electromagnetic valve (20) is arranged on a pipeline connected with the nitrogen steel cylinder (21), and an air valve (19) is arranged on a pipeline connected with the air pump (24); the top of the reactor is provided with a DO probe (14) and a reactor sampling port (15), the outer side of the reactor is provided with a hollow temperature control jacket (16), the lower part of the temperature control jacket is provided with a temperature control jacket water inlet (18) communicated with the inner cavity, and the upper part of the temperature control jacket water outlet (17) communicated with the inner cavity; the water bath pot (2) is connected with a water outlet (17) of the temperature control jacket and a water inlet (18) of the temperature control jacket to form a circulation loop; the water inlet (6) of the reactor is connected with a water inlet barrel feeding pipe (22) through a water inlet pump (23), and the water inlet barrel feeding pipe (22) is arranged in the water inlet barrel (4);
the device is also provided with a control cabinet (3), wherein the control cabinet (3) is connected with the DO probe (14), an air valve (19) and an electromagnetic valve (20);
the enrichment culture conditions of the enrichment device comprise dissolved oxygen of less than 1mg/L, a culture medium with ammonia nitrogen of less than or equal to 0.5mM, a temperature of 25-30 ℃, and whole-course nitrifying bacteria seed mud which accounts for 10% -20% of the effective volume of the reactor (1) is inoculated in the reactor (1).
2. The MBR whole course nitrifying bacteria enrichment device capable of automatically controlling dissolved oxygen according to claim 1, wherein: the reactor (1) is internally filled with a filler (13).
3. An MBR whole course nitrifying bacteria enriching method using the device of claim 2, characterized by comprising the following steps: inoculating whole course nitrifying bacteria seed mud in the reactor (1), wherein the proportion of the whole course nitrifying bacteria seed mud in the effective volume of the reactor (1) is 10-20%; injecting a low-concentration culture medium with ammonia nitrogen of less than or equal to 0.5mM into the reactor (1), and culturing and enriching microorganisms to ensure that the whole course nitrifying bacteria are attached to the surface of the filler to form a biological film; in the enrichment process, a continuous water inlet and continuous water outlet mode is adopted, and microorganisms are completely intercepted in the bioreactor through the built-in membrane module (7) during water outlet; meanwhile, the dissolved oxygen amount of the culture medium in the reactor is detected through a DO probe (14), and the air amount and the nitrogen amount are adjusted through regulating and controlling an air valve (19) and an electromagnetic valve (20), so that the dissolved oxygen in the reactor (1) is maintained at the level lower than 1 mg/L; the temperature of the culture medium is controlled to be 25-30 ℃.
4. The method as claimed in claim 3, wherein the aeration is intermittently performed by using the aeration tray to control oxygen and stir and prevent the membrane module from being clogged.
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| CN109851065B (en) * | 2019-03-22 | 2023-09-22 | 浙江大学 | Sand filter type whole-course nitrifying bacteria enrichment device and method thereof |
| CN111268794B (en) * | 2020-03-01 | 2022-07-26 | 浙江富春紫光环保股份有限公司 | Automatic feeding system and storage tank of strain for sewage treatment system |
| CN111268793B (en) * | 2020-03-02 | 2022-05-10 | 浙江富春紫光环保股份有限公司 | Enrichment device and emergency feeding method of nitrobacteria for sewage treatment |
| CN113651416B (en) * | 2021-08-06 | 2022-07-19 | 浙江大学 | Suspended state whole-course nitrifying bacteria continuous flow enrichment device and method |
| CN113772803B (en) * | 2021-08-27 | 2022-07-19 | 海南大学 | Device for culturing free-state high-abundance complete nitrifying bacteria |
| CN114105296B (en) * | 2021-11-12 | 2023-07-25 | 海南大学 | Device and method for deep denitrification based on low-oxygen complete nitrification and coupling of internal carbon source short-range denitrification anaerobic ammonia oxidation |
| CN114804341A (en) * | 2022-04-29 | 2022-07-29 | 华北电力大学 | Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application |
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