CN112694170A - Method and device for improving anaerobic ammonia oxidation denitrification effect and operation stability at low temperature - Google Patents
Method and device for improving anaerobic ammonia oxidation denitrification effect and operation stability at low temperature Download PDFInfo
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 6
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- 239000001301 oxygen Substances 0.000 claims description 4
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- 229910015667 MoO4 Inorganic materials 0.000 claims description 2
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- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/282—Anaerobic digestion processes using anaerobic sequencing batch reactors
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- C02F3/006—Regulation methods for biological treatment
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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Abstract
A method and a device for improving the denitrification effect and the operation stability of an anaerobic ammonia oxidation process at low temperature belong to the field of sewage treatment. The water distribution tank (1) is connected with the low-temperature tank (2) through a first peristaltic pump (4), is conveyed into the reaction area through a second peristaltic pump (20) through a water inlet pipe (7), and monitors and controls the conveyed water quantity through a rotor flow meter (6) and a valve (5) respectively; stirring slowly by using a stirrer (17); the outlet water is conveyed outwards through a water outlet (10); circulating water cooled by the low-temperature tank (2) enters the temperature control interlayer (3) through the backflow water inlet pipe (11) and returns to the low-temperature tank (2) through the backflow water outlet pipe (12); the online measurement is carried out by a pH online electrode (15) and a DO online electrode (16). The characteristic of the anaerobic ammonia oxidation biomembrane at low temperature (6-16 ℃) is strengthened by adding the high-density anaerobic ammonia oxidation granular sludge, and the stable operation of the anaerobic ammonia oxidation process at low temperature is ensured.
Description
Technical Field
The invention belongs to the field of sewage treatment, and particularly relates to a method and a device for improving the denitrification effect and the operation stability of an anaerobic ammonia oxidation process at low temperature.
Background
Anammox technology is currently known as the most economical biological denitrification pathway,its main advantage lies in: no need of external addition of organic carbon source, low energy consumption and operation cost, etc. However, the anaerobic ammonium oxidation bacteria have slow proliferation, are sensitive to environmental (such as temperature) conditions and have poor adaptability in adverse environments. For example, at low temperature conditions (<15 ℃), the activity of anaerobic ammonium oxidation flora is inhibited, and the reactor is difficult to stably operate for a long time. Li Ming-Cong et al (2019) studied the influence of seasonal temperature change on the anammox denitrification performance, and the results show that the continuous decrease of the temperature in winter has a significant adverse effect on the denitrification efficiency of the reactor, the total nitrogen removal rate is reduced by 65% from 84.5%, and the microbial activity is significantly reduced; in the study of Zhang Chi et al (2019), when the nitrogen load is 1.60 kg. Nm-3d-1In the meantime, the denitrification rate of the anaerobic ammonia oxidation CSTR reactor at 16 ℃ is reduced to 15.07 percent. Some researchers have carried out related research and put forward corresponding control strategies, such as modifying the type of the reactor, adding biological carriers into the reactor, introducing 1000Hz intermediate-frequency pulse current into the anaerobic ammonia oxidation reactor, or carrying out periodic high-temperature impact, etc., however, these methods cannot fundamentally regulate the physiological behavior of ANAMMOX microorganisms, and have complex operation and lack of universality. The research researches the influence of the exogenous high-density ANAMMOX granular sludge on the denitrification performance of the ANAMMOX biomembrane process at low temperature from a brand new angle, and carries out deep mechanism analysis.
Quorum Sensing (QS) exists among bacteria, and the QS communicates through signal molecules, and when the concentration of the signal molecules reaches a certain threshold value, the signal molecules enter different cytoplasms to activate the expression of target genes and regulate and control the physiological behaviors of the bacteria. Among them, N-Acyl Homoserine Lactones (AHLs) are a broad signal molecule to be studied, and QS systems based on AHLs are widely used to regulate various microbial behaviors, including biofilm formation, Extracellular Polymeric Substrates (EPS) secretion, nitrogen transformation, microbial community construction, and the like. Zhang jing et al (2019) find that N-octanoyl-DL-homoserine lactone (C8-HSL) can remarkably improve the settling property of anaerobic ammonia oxidation granular sludge, effectively control the sludge to float upwards, and improve the operation stability of a reactor and the total nitrogen removal rate; xiong Fuzhong et al (2020) adds exogenous AHLs (C6-HSL and 3OC6-HSL) to obviously increase the contents of PS and PN in EPS and Soluble Microbial Products (SMP) of Pseudomonas, Comamonas and other bacteria in anaerobic ammonia oxidation sludge, obviously quickens the biofilm formation process on a carrier, and forms thicker and better structure biofilm; liu Lingjie et al (2020) found that by stimulating the release of AHLs in an anammox system, the activity of anammox bacteria and the content of extracellular polymeric substances can be increased, the quorum sensing effect can be enhanced, and the denitrification performance can be further improved. However, modulating biofilm process performance by purifying AHLs faces the problem of expensive AHLs costs. Research in recent years shows that some microorganisms have the potential to release AHLs at higher concentrations, but related research is very limited, and the screening of pure cultured microorganisms or activated sludge capable of releasing AHLs with high efficiency still remains to be explored.
Relevant research shows that ANAMMOX sludge can release AHLs, but the concentration of the released AHLs is low, and the ANAMMOX sludge with high density can release the AHLs with high concentration by intensive culture and screening. Compared with the mode of adding purified exogenous AHLs into a reactor, the mode of adding the high-density ANAMMOX granular sludge into the reactor in a biological strengthening way can improve the bacterial activity and accelerate the formation of a biofilm, simultaneously maintain the diversity of sludge and bacteria in the reactor, ensure enough biomass in the environment, ensure the treatment performance of the system at low temperature, and have the advantages of low cost and easy operation.
Disclosure of Invention
In order to solve the problems that the growth of microorganisms is limited, the activity is reduced, a reactor is difficult to keep long-term stable operation and the denitrification is incomplete because the anaerobic ammonia oxidation process is influenced by low temperature in the process of treating nitrogen-containing wastewater, a method for improving the denitrification effect and the operation stability of the anaerobic ammonia oxidation process at low temperature by using the quorum sensing of exogenous high-density ANAMMOX granular sludge is provided. The main innovation point is that the attachment performance and activity of the anaerobic ammonia oxidation biomembrane at low temperature are improved by using the strain fed-batch technology, namely the characteristic of the anaerobic ammonia oxidation biomembrane at low temperature (6-16 ℃) is strengthened by using the high-density anaerobic ammonia oxidation granular sludge, and a good method is provided for the stable operation of the anaerobic ammonia oxidation technology at low temperature and the improvement of the denitrification effect.
A device for improving the denitrification effect and the operation stability of an anaerobic ammonia oxidation process at low temperature by utilizing quorum sensing of exogenous high-density ANAMMOX granular sludge is characterized by comprising the following components: the device comprises a water distribution tank (1), a low-temperature tank (2), a temperature control interlayer (3), a first peristaltic pump (4), a valve (5), a rotor flowmeter (6), a water inlet pipe (7), an isolation net (8), a pall ring (9), a water outlet (10), a backflow water inlet pipe (11), a backflow water outlet pipe (12), an exhaust hole (13), a sludge exchange pipe outlet (14), a pH online electrode (15), a DO online electrode (16), a stirrer (17), high-density anaerobic ammonia oxidation granular sludge (18) and a main body reaction device (19); a second peristaltic pump (20), a third peristaltic pump (21), a fourth peristaltic pump (22) and a fifth peristaltic pump (23).
The main body reaction device (19) is cylindrical, in order to reduce the interference of pall rings to suspended sludge sedimentation and water outlet drainage in the sedimentation process, 1/5 areas which are parallel to each other at the left and right are isolated by an isolation net (8) and are used as sedimentation areas, other areas are reaction areas, pall rings (9) which are used as suspended fillers are arranged in the reaction areas in the main body reaction device (19) and float in the reactor, a stirrer (17) and high-density anaerobic ammonia oxidation granular sludge (18) are arranged in the reaction areas of the main body reaction device (19), an exhaust hole (13) is arranged at the top of the main body reaction device (19), and a pH online electrode (15) and a DO online electrode (16) are also arranged in the reaction areas of the main body reaction device (19); the water distribution tank (1) is connected with the low-temperature tank (2) through a first peristaltic pump (4), and the low-temperature tank (2) is connected with a reaction area of a main body reaction device (19) through a second peristaltic pump (20), a rotor flow meter (6) and a valve (5) by adopting a water inlet pipe (7); a water outlet (10) of the main body reaction device (19) is connected with a third peristaltic pump (21), a mud changing outlet is arranged at the lower part of the main body reaction device (19), and the mud changing outlet is connected with a fourth peristaltic pump (22) through a mud changing pipe outlet (14);
a temperature control interlayer (3) at the outer side of the main body reaction device (19) is provided with a backflow water inlet pipe (11) and a backflow water outlet pipe (12), and the backflow water inlet pipe (11) is connected with the low-temperature tank (2) by a fifth peristaltic pump (23); the backflow water outlet pipe (12) is directly connected with the low-temperature tank (2).
The backflow water outlet pipe (12) is positioned on the side surface of the middle upper part of the temperature control interlayer (3), and the backflow water inlet pipe (11) is positioned on the side surface of the middle lower part of the temperature control interlayer (3).
The outer side of the main body reaction device (19) is provided with a temperature control interlayer (3).
The main body reaction device (19) is made of organic glass, and the diameter-height ratio is 1: 4.
Water in the water distribution tank (1) is conveyed into the low-temperature tank (2) by a first peristaltic pump (4), and is conveyed into the reaction area by a second peristaltic pump (20) through a water inlet pipe (7) after the water temperature reaches a specified temperature, and the conveyed water amount is monitored and controlled by a rotameter (6) and a valve (5) respectively; in the reaction stage, a stirrer (17) is used for stirring slowly; the outlet water is conveyed outwards through a water outlet (10); circulating water cooled by the low-temperature tank (2) enters the temperature control interlayer (3) through the backflow water inlet pipe (11) and then returns to the low-temperature tank (2) through the backflow water outlet pipe (12), so that low-temperature control of the reactor is realized; the pH and DO in the reaction zone are measured on line through a pH on-line electrode (15) and a DO on-line electrode (16).
A method for realizing denitrification of an anaerobic ammonia oxidation biomembrane process at low temperature by using the reactor is also characterized by comprising the following steps:
domestication culture of high-density ANAMMOX granular sludge
Inoculating common ANAMMOX granular sludge (the sludge density is 0.98-1.05mg/L, the sludge biomass concentration MLVSS is 33.07-39.24gVSS/L) by adopting an Upflow Anaerobic Sludge Blanket (UASB) reactor, feeding water into the reactor to obtain high-nitrogen-concentration wastewater, acclimatizing and culturing the high-biomass-density ANAMMOX granular sludge by controlling the high-water-feeding substrate concentration of a reaction system, and adopting a continuous water feeding and draining mode;
the concentration of ammonia nitrogen and nitrite nitrogen in inlet water of the reactor is maintained at 450-600mg/L, the ammonia nitrogen and nitrite nitrogen in the reactor are kept at higher concentration, and the inhibition effect on the activity of the anammox bacteria is avoided. By detecting the concentration of the ammonia nitrogen and the nitrite nitrogen in the effluent, when the concentration of the effluent is lower than 10mg/L, the concentration of the ammonia nitrogen and the nitrite nitrogen in the inlet water of the reactor is increased, the volume load of the inlet water is increased, and the concentration of the ammonia nitrogen and the nitrite nitrogen in the reactor is higher than 450 mg/L; when the concentration of the outlet water is higher than 20mg/L, the concentrations of the ammonia nitrogen and the nitrite nitrogen in the inlet water are properly reduced, and the ANAMMOX bacteria are prevented from being inhibited; after the ANAMMOX-UASB reactor is operated for 60 days, ANAMMOX granular sludge with high sludge density is obtained, wherein the sludge density is 1.22-1.29mg/L, and the sludge biomass concentration (MLVSS) is 48.12-56.16 gVSS/L;
(II) SBBR reactor set-up
The anaerobic ammonia oxidation biomembrane in the SBBR reactor for low-temperature denitrification is taken from an anaerobic ammonia oxidation UASB reactor cultured for more than one year, and the initial biomass concentration in an SBBR main body reaction device (19) after inoculation is about 3 g-VSSL-1(ii) a The reactor is covered by a black shading heat-insulating layer, so that the influence of light on the culture of the ANAMMOX biomembrane is avoided; the operation mode is sequencing batch, comprising water feeding, reaction, precipitation, water drainage and idling; wherein the water is fed for 15min, the anaerobic reaction stage is 7h, the precipitation stage is 30min, the water drainage stage is 5min, the standing is 10min, the water is fed for 6L, the operation time of each period is 8h, the operation time is 3 periods each day, and the hydraulic retention time is 12-14 h; starting a stirrer to stir slowly in an anaerobic reaction stage, wherein the stirring speed is 60 rmp; controlling water inflow of the reactor by a second peristaltic pump, setting the temperature of water in the reaction zone to be 6 ℃ at the lowest temperature and not more than 16 ℃ at the highest temperature, controlling the temperature of the inflow water to keep the low temperature state, reducing the temperature of experimental inflow water to be below 16 ℃ through a low-temperature tank, and then feeding the experimental inflow water into the reactor, wherein the concentration of dissolved oxygen in an anaerobic stage is controlled to be 6 DEG in the reaction process<0.05mg/L；
The invention adopts artificially synthesized wastewater, ammonia nitrogen, nitrite nitrogen and other nutrient substances are regularly configured according to a certain proportion, and the change of the quality of the inlet water caused by too long water distribution time is prevented; controlling the pH value of inlet water of the reactor to be 7.5-8.0; the artificial synthetic wastewater comprises the following components: 25-45 mg/L KH2PO4，0.45～0.68g/L NaHCO3，90～120mg/L MgSO4·7H2O and 30-55 mg/L CaCl2·2H2O; according to the required ammonia nitrogen concentration, the ammonia nitrogen and the nitrite nitrogen are mixed according to the proportion of 1: 1.20-1.32, adding NH into water in the form of ammonium chloride and sodium nitrite4 +-N (in terms of N) 45-67 mg.L-1、NO2 –-N (calculated as N) 55-90 mg.L-1(ii) a Simultaneously, 1mL/L of trace element solution I and trace element solution II are also added, and the trace element solution I comprises the following components: 300g/L EDTA and 3.00g/LFeSO4(ii) a The microelement solution II comprises 8.00g/L EDTA and 0.2g/L ZnSO4·4H2O、0.5g/L MnCl2·4H2O、0.005g/LH3BO4、0.15g/LCuSO4·5H2O、0.15g/LNa2MoO4·2H2O、0.12g/LNa2SeO4·10H2O and 0.12g/L NiCl2·6H2O；
With N2-CO2(95/5%) the synthesis wastewater is flushed with a gaseous mixture to remove dissolved oxygen DO and maintain anaerobic conditions in the reactor.
In the invention, the SBBR reactor is provided with a circulating water temperature control interlayer on the outer layer. As a constant temperature reflux system, water in the low-temperature tank enters from the bottom of the outer layer of the reactor, and water flows out from the top of the interlayer and flows back to the low-temperature tank. The water in the inner layer of the reactor enters from the top and exits from the bottom, and the anaerobic ammonia oxidation bacteria are attached to the pall ring to grow. The upper part of the reactor is provided with a sealing device to ensure that the whole device is in an anaerobic state. Meanwhile, a valve is arranged at the top of the reactor to discharge the product N of the anaerobic ammonia oxidation reaction2。
Taking out a proper amount of cultured high-density anaerobic ammonia oxidation granular sludge from the step (I), and adding the sludge into a reaction zone of a main reaction device (19) after the drainage stage is finished when the step (II) is operated stably for a long time; adding high-density sludge once every 6 circulation periods, and simultaneously discharging the same amount of original sludge; each 100mL of sludge was added and discharged per 8L volume.
There are 6 genera among anammox bacteria, Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus, Candidatus Jettenia, Candidatus Anammoxymicrobioum, and Candidatus Scalindna, respectively. Among them, Candidatus Jettenia is more sensitive to strong water temperature fluctuation, while ca. brocadia, ca. kuenenia and ca. anammoxobium anaerobes can well adapt to strong temperature disturbance. Due to quorum sensing action, AHLs can increase the contents of PS and PN in EPS and Soluble Microbial Products (SMP) of Ca, Brocadia, Ca, Kuenenia and other bacteria in anaerobic ammonia oxidation sludge, accelerate the attachment of a biological membrane on a carrier and form a thicker biological membrane with a good structure.
Anaerobic ammonia oxidation sludge contains a large amount of AHLs, and Anamox sludge with high density is cultured through domestication, the content of effective AHLs in the sludge is enhanced, the high-density granular sludge is added into a reactor, the bacterial community optimization can be promoted, the bacterial quantity required by the environment is supplemented, meanwhile, some potential growth factors essential to functional bacteria in an anaerobic ammonia oxidation system are introduced, and the resistance and the restoring force of the bacterial community to the external low-temperature environment are enhanced. Due to the potential quorum sensing effect, the communication between bacteria is promoted through the mutual transmission of chemical signals between the bacteria, the functional flora abundance can be maintained, and the flora diversity and the flora structure can be improved. The addition of the enhanced Anammox granular sludge can promote the attachment of anaerobic ammonia oxidation biomembranes of the reactor and improve the activity of the flora, so that the QS system based on the AHLs has important influence on the formation of the biomembranes and the establishment of the microbial communities in the bioaugmentation reactor.
The exogenous high-density ANAMMOX granular sludge is added into an ANAMMOX-SBBR reactor, namely a main reaction device (19), so that the denitrification effect and the operation stability of the ANAMMOX-SBBR reactor are kept at low temperature, the resistance of the anaerobic ammonia oxidation biomembrane process to low-temperature impact is improved, and compared with the traditional anaerobic ammonia oxidation biomembrane process, the method has the following advantages:
(1) through biological enhancement, the ANAMMOX biofilm attachment capacity is improved, and the reactor is started faster than the traditional reactor.
(2) Improving the activity and quorum sensing action of anaerobic ammonium oxidation flora at low temperature.
(3) The device has small volume, simple operation and strong low-temperature impact resistance.
(4) The feeding amount and the discharging amount of the high-density sludge can be flexibly adjusted according to the water temperature, the water quality and the operation conditions.
(5) The cost is low, and the device is easy to operate.
Drawings
FIG. 1 is a schematic view of the structure of the apparatus of the present invention
In the figure: the device comprises a water distribution tank 1, a low-temperature tank 2, a temperature control interlayer 3, a peristaltic pump 4, a valve 5, a rotor flowmeter 6, a water inlet pipe 7, an isolation net 8, a pall ring 9, a water outlet 10, a backflow water inlet pipe 11, a backflow water outlet pipe 12, an exhaust hole 13, a mud changing pipe 14, a pH online electrode 15, a DO online electrode 16, a stirrer 17, high-density anaerobic ammonia oxidation granular sludge 18, a main body reaction device 19, a second peristaltic pump 20, a third peristaltic pump 21, a fourth peristaltic pump 22 and a fifth peristaltic pump 23.
The specific implementation method comprises the following steps:
the present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples.
Example 1: a device for improving denitrification effect and operation stability of anaerobic ammonia oxidation process at low temperature by using quorum sensing of exogenous high-density ANAMMOX granular sludge is shown in figure 1, wherein a main reaction device is cylindrical, and a pall ring (9) serving as a suspended filler is arranged in the main reaction device and floats in a reactor; in order to reduce the interference of pall ring on the suspended sludge sedimentation and water outlet drainage in the sedimentation process, an 1/5 area is isolated by an isolation net (8) and used as a sedimentation area; water in the water distribution tank (1) is conveyed into the low-temperature tank (2) by a peristaltic pump (4), and after the water temperature reaches a specified temperature, the water is conveyed into the SBBR reactor by the peristaltic pump (4) through a water inlet pipe (7), and the conveyed water amount is monitored and controlled by a rotor flow meter (6) and a valve (5) respectively; in the anaerobic reaction stage, a stirrer (17) is used for stirring slowly; the effluent after reaction is conveyed outwards through a water outlet (10); circulating water cooled by the low-temperature tank (2) enters the temperature control interlayer (3) through the backflow water inlet pipe (11) and then returns to the low-temperature tank (2) through the backflow water outlet pipe (12), so that the accurate control of the outer layer temperature of the reactor is realized; the pH and DO in the reactor are measured on line through a pH on-line electrode (15) and a DO on-line electrode (16). The effective volume of the SBBR used in this example is 8L, and the volume of the reactor can be proportionally expanded as required.
Example 2: the method for promoting quorum sensing and improving the denitrification performance of the anaerobic ammonia oxidation biofilm process by using the exogenous high-density granular sludge by regularly putting the high-density granular sludge cultured in the ANAMMOX-UASB reactor into the SBBR reactor in the embodiment 1 through a sludge changing pipe comprises the following specific steps:
(1) domestication culture of high-density ANAMMOX granular sludge
Inoculating common ANAMMOX granular sludge (the sludge density is 0.98-1.05mg/L, the sludge biomass concentration MLVSS is 33.07-39.24gVSS/L) by adopting an Upflow Anaerobic Sludge Blanket (UASB) reactor, feeding water into the reactor to obtain high-nitrogen-concentration wastewater, acclimatizing and culturing the high-biomass-density ANAMMOX granular sludge by controlling the high-water-feeding substrate concentration of a reaction system, and adopting a continuous water feeding and draining mode;
the concentration of ammonia nitrogen and nitrite nitrogen in inlet water of the reactor is maintained at 450-600mg/L, the ammonia nitrogen and nitrite nitrogen in the reactor are kept at higher concentration, and the inhibition effect on the activity of the anammox bacteria is avoided. By detecting the concentrations of the ammonia nitrogen and the nitrite nitrogen in the effluent, when the concentration of the effluent is lower than 10mg/L, the concentrations of the ammonia nitrogen and the nitrite nitrogen in the inlet water of the reactor are increased, the volume load of the inlet water is increased, and the concentrations of the ammonia nitrogen and the nitrite nitrogen in the reactor are higher than 450 mg/L; when the concentration of the outlet water is higher than 20mg/L, the concentrations of the ammonia nitrogen and the nitrite nitrogen in the inlet water are properly reduced, and the ANAMMOX bacteria are prevented from being inhibited; after the ANAMMOX-UASB reactor is operated for 60 days, ANAMMOX granular sludge with high sludge density is obtained, the sludge density is 1.22-1.29mg/L, and the sludge biomass concentration (MLVSS) is 48.12-56.16 gVSS/L.
The manual water distribution component comprises: 25mg/L KH2PO4，0.45g/L NaHCO3，90mg/L MgSO4·7H2O and 30mg/L CaCl2·2H2O；NH4 +-N (in terms of N) 450-600 mg.L-1、NO2 –550-N (in terms of N) 800 mg. L-1。
Solution of trace elements I: 1mL/L, trace element II solution: 1 mL/L. (solution of trace element I and solution of trace element II in the same invention)
(2) ANAMMOX-SBBR reactor operation
1. The reactor water inlet mainly comprises the following components:
preparing water quality parameters of simulated domestic sewage by reactor water inflow: 25mg/L KH2PO4，0.45g/L NaHCO3，90mg/L MgSO4·7H2O and 30mg/L CaCl2·2H2O；NH4 +-N (calculated as N) 49-100 mg.L-1、NO2 –-N (calculated as N) 62-136 mg.L-1。
Solution of trace elements I: 1mL/L, trace element II solution: 1mL/L, and the pH is kept between 7.5 and 8.0.
3. Device operating condition control
The ANAMMOX-SBBR reactor (volume of 8L in the embodiment) is inoculated with anaerobic ammonia oxidation biomembrane (biomembrane attaching carrier is pall ring, filling ratio is 30%), simulated wastewater is injected into the device from a water inlet pipe 7 through a peristaltic pump, and the device is sealed to ensure anaerobic condition. 100mL of high-density anaerobic ammonia oxidation granular sludge is added into the reactor through a sludge changing pipe 14 every 6 circulation periods, then the same amount of original sludge is discharged, and the AHLs in the exogenous sludge are utilized to generate quorum sensing effect to participate in biomembrane denitrification at low temperature. Sampling and detecting are carried out from the water outlet pipe 10 in the water discharging stage of each reaction period. The SBBR reactor is operated in a sequencing batch mode and comprises water inlet, reaction, precipitation, water drainage and idling; wherein the water is fed for 15min, the anaerobic reaction stage is 7h, the precipitation stage is 30min, the water discharging stage is 5min, the standing is 10min, the water is fed for 6L, the operation time of each period is 8h, the operation time is 3 periods each day, the hydraulic retention time is 12h, and the pH value is controlled to be 7.5-8.0. The device is provided with a low-temperature tank circulating water temperature control interlayer and a sealing device to ensure different low-temperature environments. The reactor conditions are divided into four different cases, R1, R2, R3 and R4 operating at different temperatures: reactor R1 was run at 16 ℃ for 180 days, reactor R2 at 11 ℃ for 120 days, reactor R3 at 7 ℃ for 80 days, and reactor R4 at 6 ℃ for 60 days.
4. Result of device operation
Through detection, the anaerobic ammonia oxidation biomembrane in the reactor after a small amount of high-density anaerobic ammonia oxidation granular sludge is added has an obvious nitrogen removal effect on nitrogen-containing wastewater at low temperature. When the water temperature is 16 ℃ (lasting 180 days), the denitrification performance of the reactor R1 is stable (the average total nitrogen removal rate reaches about 94%); when the water temperature is 11 ℃ (lasting 120 days), the denitrification performance of the reactor R2 is stable (the average total nitrogen removal rate reaches about 91%); when the water temperature is 7 ℃ (lasting 80 days), the denitrification performance of the reactor R4 is stable (the average total nitrogen removal rate reaches about 87%); when the water temperature is 6 ℃ (lasting 60 days), the denitrification performance of the reactor R5 is stable (the average total nitrogen removal rate reaches about 83%).
According to the invention, the high-density anaerobic ammonia oxidation granular sludge is added from an external source, so that the adhesion performance and activity of the ANAMMOX biomembrane are promoted, and the quorum sensing effect generated by the high-density anaerobic ammonia oxidation granular sludge is utilized to improve the operation stability and denitrification efficiency of the ANAMMOX biomembrane process in the reactor. The device and the method have flexible application and simple operation, and can flexibly transform the reaction device according to actual needs.
Claims (4)
1. A device for improving the denitrification effect and the operation stability of an anaerobic ammonia oxidation process at low temperature by utilizing a quorum sensing mechanism of exogenous high-density anaerobic ammonia oxidation (ANAMMOX) granular sludge is characterized by comprising the following components: the device comprises a water distribution tank (1), a low-temperature tank (2), a temperature control interlayer (3), a first peristaltic pump (4), a valve (5), a rotor flowmeter (6), a water inlet pipe (7), an isolation net (8), a pall ring (9), a water outlet (10), a backflow water inlet pipe (11), a backflow water outlet pipe (12), an exhaust hole (13), a sludge exchange pipe outlet (14), a pH online electrode (15), a DO online electrode (16), a stirrer (17), high-density anaerobic ammonia oxidation granular sludge (18) and a main body reaction device (19); a second peristaltic pump (20), a third peristaltic pump (21), a fourth peristaltic pump (22) and a fifth peristaltic pump (23);
the main body reaction device (19) is cylindrical, in order to reduce the interference of pall rings to suspended sludge sedimentation and water outlet drainage in the sedimentation process, 1/5 areas which are parallel to each other at the left and right are isolated by an isolation net (8) and are used as sedimentation areas, other areas are reaction areas, pall rings (9) which are used as suspended fillers are arranged in the reaction areas in the main body reaction device (19) and float in the reactor, a stirrer (17) and high-density anaerobic ammonia oxidation granular sludge (18) are arranged in the reaction areas of the main body reaction device (19), an exhaust hole (13) is arranged at the top of the main body reaction device (19), and a pH online electrode (15) and a DO online electrode (16) are also arranged in the reaction areas of the main body reaction device (19); the water distribution tank (1) is connected with the low-temperature tank (2) through a first peristaltic pump (4), and the low-temperature tank (2) is connected with a reaction area of a main body reaction device (19) through a second peristaltic pump (20), a rotor flow meter (6) and a valve (5) by adopting a water inlet pipe (7); a water outlet (10) of the main body reaction device (19) is connected with a third peristaltic pump (21), a mud changing outlet is arranged at the lower part of the main body reaction device (19), and the mud changing outlet is connected with a fourth peristaltic pump (22) through a mud changing pipe outlet (14);
a temperature control interlayer (3) is arranged on the outer side of the main body reaction device (19), the temperature control interlayer (3) is provided with a backflow water inlet pipe (11) and a backflow water outlet pipe (12), and the backflow water inlet pipe (11) is connected with the low-temperature tank (2) through a fifth peristaltic pump (23); the backflow water outlet pipe (12) is directly connected with the low-temperature tank (2).
2. The device for improving the denitrification effect and the operation stability of the anaerobic ammonia oxidation process at low temperature by utilizing the quorum sensing mechanism of exogenous high-density ANAMMOX granular sludge as claimed in claim 1, wherein the backflow water outlet pipe (12) is positioned on the middle upper side surface of the temperature control interlayer (3), and the backflow water inlet pipe (11) is positioned on the middle lower side surface of the temperature control interlayer (3).
3. The device for improving the denitrification effect and the operation stability of the anaerobic ammonia oxidation process at low temperature by utilizing the quorum sensing mechanism of the exogenous high-density ANAMMOX granular sludge as claimed in claim 1, wherein the main reaction device (19) is made of organic glass and has a diameter-height ratio of 1: 4.
4. A method for denitrification in a low temperature anammox biofilm process using the apparatus of claim 1 or 2, comprising the steps of:
domestication culture of high-density ANAMMOX granular sludge
Inoculating common ANAMMOX granular sludge (the sludge density is 0.98-1.05mg/L, the sludge biomass concentration MLVSS is 33.07-39.24gVSS/L) by adopting an Upflow Anaerobic Sludge Blanket (UASB) reactor, feeding water into the reactor to obtain high-nitrogen-concentration wastewater, acclimatizing and culturing the high-biomass-density ANAMMOX granular sludge by controlling the high-water-feeding substrate concentration of a reaction system, and adopting a continuous water feeding and draining mode;
the concentration of ammonia nitrogen and nitrite nitrogen in inlet water of the reactor is maintained at 450-600mg/L, the ammonia nitrogen and nitrite nitrogen in the reactor are kept at higher concentration, and the inhibition effect on the activity of the anammox bacteria is avoided. By detecting the concentrations of the ammonia nitrogen and the nitrite nitrogen in the effluent, when the concentration of the effluent is lower than 10mg/L, the concentrations of the ammonia nitrogen and the nitrite nitrogen in the inlet water of the reactor are increased, the volume load of the inlet water is increased, and the concentrations of the ammonia nitrogen and the nitrite nitrogen in the reactor are higher than 450 mg/L; when the concentration of the outlet water is higher than 20mg/L, the concentrations of the ammonia nitrogen and the nitrite nitrogen in the inlet water are properly reduced, and the ANAMMOX bacteria are prevented from being inhibited; after the ANAMMOX-UASB reactor is operated for 60 days, ANAMMOX granular sludge with high sludge density is obtained, wherein the sludge density is 1.22-1.29mg/L, and the sludge biomass concentration (MLVSS) is 48.12-56.16 gVSS/L;
(II) SBBR reactor set-up
The anaerobic ammonia oxidation biomembrane in the SBBR reactor for low-temperature denitrification is taken from an anaerobic ammonia oxidation UASB reactor cultured for more than one year, and the initial biomass concentration in an SBBR main body reaction device (19) after inoculation is about 3 g-VSSL-1(ii) a The reactor is covered by a black shading heat-insulating layer, so that the influence of light on the culture of the ANAMMOX biomembrane is avoided; the operation mode is sequencing batch, comprising water feeding, reaction, precipitation, water drainage and idling; wherein the water is fed for 15min, the anaerobic reaction stage is 7h, the precipitation stage is 30min, the water drainage stage is 5min, the standing is 10min, the water is fed for 6L, the operation time of each period is 8h, the operation time is 3 periods each day, and the hydraulic retention time is 12-14 h; starting a stirrer to stir slowly in an anaerobic reaction stage, wherein the stirring speed is 60 rmp; controlling water inflow of the reactor by a second peristaltic pump, setting the temperature of water in the reaction zone to be 6 ℃ at the lowest temperature and not more than 16 ℃ at the highest temperature, controlling the temperature of the inflow water to keep the low temperature state, reducing the temperature of experimental inflow water to be below 16 ℃ through a low-temperature tank, and then feeding the experimental inflow water into the reactor, wherein the concentration of dissolved oxygen in an anaerobic stage is controlled to be 6 DEG in the reaction process<0.05mg/L；
Ammonia nitrogen, nitrite nitrogen and other nutrient substances are regularly configured according to a certain proportion, so that the change of the quality of inlet water caused by too long water distribution time is prevented; controlling the pH value of inlet water of the reactor to be 7.5-8.0; the artificial synthetic wastewater comprises the following components: 25-45 mg/L KH2PO4，0.45～0.68g/L NaHCO3，90～120mg/L MgSO4·7H2O and 30-55 mg/L CaCl2·2H2O; according to the required ammonia nitrogen concentration, the ammonia nitrogen and the nitrite nitrogen are mixed according to the proportion of 1: 1.20-1.32, adding NH into water in the form of ammonium chloride and sodium nitrite4 +-N (in terms of N) 45-67 mg.L-1、NO2 –-N (calculated as N) 55-90 mg.L-1(ii) a Simultaneously, 1mL/L of trace element solution I and trace element solution II are also added, and the trace element solution I comprises the following components: 3.00g/L EDTA and 3.00g/LFeSO4(ii) a The microelement solution II comprises 8.00g/L EDTA and 0.2g/L ZnSO4·4H2O、0.5g/L MnCl2·4H2O、0.005g/LH3BO4、0.15g/LCuSO4·5H2O、0.15g/LNa2MoO4·2H2O、0.12g/LNa2SeO4·10H2O and 0.12g/L NiCl2·6H2O；
With N2-CO2(95/5%) the synthesis wastewater is flushed with a gaseous mixture to remove Dissolved Oxygen (DO) and to maintain anaerobic conditions in the reactor;
taking out a proper amount of cultured high-density anaerobic ammonia oxidation granular sludge from the step (I), and adding the sludge into a reaction zone of a main reaction device (19) after the drainage stage is finished when the step (II) is operated stably for a long time; adding high-density granular sludge once every 6 circulation periods, and simultaneously discharging the same amount of original sludge; each 100mL of sludge was added and discharged per 8L volume.
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