CN106632727A - A hyaluronic acid extraction process - Google Patents
A hyaluronic acid extraction process Download PDFInfo
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- CN106632727A CN106632727A CN201610845404.XA CN201610845404A CN106632727A CN 106632727 A CN106632727 A CN 106632727A CN 201610845404 A CN201610845404 A CN 201610845404A CN 106632727 A CN106632727 A CN 106632727A
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- hyaluronic acid
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- extraction technique
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A hyaluronic acid extraction process is disclosed. The process includes steps of 1) adjusting pH, 2) performing centrifugation, 3) performing membrane treatment, 4) performing decoloring treatment, 5) performing deposition with ethanol, 6) performing secondary membrane treatment, 7) performing secondary deposition and 8) drying. The process is a production process that can be safely and efficiently performed for long time and that is convenient to maintain. The process is simple, and the production cost is reduced.
Description
Technical field
The present invention relates to membrane technology field, particularly a kind of hyaluronan extraction technique.
Background technology
Hyaluronic acid, also known as uronic acid, basic structure is by two dissacharide units D-Glucose aldehydic acid and N- Acetylglucos
The large-scale polysaccharide of amine composition.It is different from other mucopolysaccharides, its not sulfur-bearing.Its hyalomitome molecule can carry more than 500 times
Moisture, is current recognized optimal moisturizing ingredient, in being widely applied to skin care products and cosmetics.Hyaluronic acid is unique with it
Molecular structure and physicochemical property show various important physiological functions, such as lubricating joint in body, adjust vascular wall
Permeability, regulatory protein matter, Water-Electrolyte diffusion and operating, promote wound healing etc..
Zymotic fluid is put after tank after plate-frame filtering again through centrifuge in existing process, part producer can also from
Followed by equipment is filtered again together, the enzyme liquid of collection needs further concentration because enzyme activity is than relatively low, but is because that enzyme is unique for scheming
Physicochemical property, can only concentrate under middle cryogenic conditions, enzyme can be inactivated under hot conditions, and traditional concentrated in vacuo, and film steams
Send out and have that energy consumption is huge, the shortcoming that time-consuming.
The content of the invention
It is an object of the invention to provide a kind of hyaluronan extraction technique.
A kind of hyaluronan extraction technique, its extraction process step includes：
Step one, regulation PH：Resulting zymotic fluid is carried out into PH regulations so as in impurity precipitated；
Step 2, centrifugal treating：The zymotic fluid adjusted after PH is put into into centrifuge carries out centrifugal treating, obtains the hyalomitome clarified
Acid fermentation liquid；
Step 3, film process：Hyaluronic acid fermentation liquor is put into into reverse osmosis membrane carries out concentration, obtains hyaluronic acid concentration
Liquid；
Step 4, desolventing technology：The hyaluronic acid concentrate for obtaining is put in organic decolouring system carries out desolventing technology, obtains
Hyaluronic acid clarified solution；
Step 5, ethanol precipitation：The hyaluronic acid clarified solution for obtaining is carried out after PH regulations, adds ethanol to be precipitated；
Step 6, secondary film process：Hyaluronic acid after ethanol precipitation is put in film carries out a chain of filtration, obtains hyaluronic acid
Crude product；
Step 7, secondary precipitation：The hyaluronic acid crude product for obtaining is put into after ethanol precipitated, hyaluronic acid is obtained；
Step 8, drying：The hyaluronic acid for obtaining is dried, hyaluronic acid finished product is obtained.
Preferably, the step one, PH regulations are carried out by sodium hydroxide solution to the zymotic fluid for obtaining.
Preferably, the step 2, is processed the zymotic fluid high speed centrifugation after PH process by supercentrifuge, and it is heavy to remove
Shallow lake impurity.
Preferably, the step 3, by the height retention under physical state of the reverse osmosis membrane to viable bacteria body, while sufficiently
The high enzyme downstream clear liquid of clarity is isolated, the effect of downstream membrane process is protected, cotton, timber of containing cellulose etc. is adopted for former
Material, through esterification and hydrolysis acetic acid, Triafol T are made（CA、CTA）, it is reprocessed into reverse osmosis membrane.
Preferably, the step 4, by organic decolouring membranous system desolventing technology is carried out, by soluble small molecular protein,
The materials such as salinity, pigment, it is 100-1000 or so that it carries out separating molecular weight cut off, and surface apertures are in nanoscale, using vinegar
Acid, Triafol T（CA、CTA）For organic decolouring membranous system of main material manufacture.
Preferably, the step 5, the precipitation salt dissolving obtained after ethanol precipitation is for further processing again.
Preferably, the step 6, is processed by microfiltration membranes, and the material of membrane component has cellulose acetate film (CA) to again
Film (PA), polyamide patent sandwich diaphragm, polysulfones (PS) material film, Kynoar (PVDF) material film are closed, and is modified
Polyacrylonitrile（PAN）Material film.
Preferably, the step 8, process is dried by drying device by the wet hyaluronic acid for obtaining.
Advantages of the present invention：
1st, filtering accuracy is high, fundamentally ensure that the degree of purity of filtrate and the formedness of quality.
2nd, membrane process is pure physical process, and without phase transformation, energy consumption is low, and system is operated at normal temperatures, overcomes heat to enzyme preparation
The destruction of active material in product.
3rd, compare traditional filtering technique, product quality is not only improved, and product yield and production efficiency have compared with
For significant raising.
4th, loss of effective components is reduced, reduces BOD and COD contents in waste water.With traditional filtration, concentration technology method phase
Than new technology can significantly save inorganic salts, organic solvent in technical process, reduce the production cost of product.
5th, the supporting control section advanced design of membranous system, so that the more aobvious simplicity of the operation and maintenance of system, significantly
Reduce the labour intensity of workman.
Description of the drawings
Accompanying drawing 1 is implementing procedure figure of the invention.
Specific embodiment
The present invention is further described in detail in conjunction with accompanying drawing 1 and specific embodiment.
Specific embodiment making step is as follows：
A kind of hyaluronan extraction technique, its extraction process step includes：
Step one, regulation PH：Resulting zymotic fluid is carried out into PH regulations so as in impurity precipitated；
Step 2, centrifugal treating：The zymotic fluid adjusted after PH is put into into centrifuge carries out centrifugal treating, obtains the hyalomitome clarified
Acid fermentation liquid；
Step 3, film process：Hyaluronic acid fermentation liquor is put into into reverse osmosis membrane carries out concentration, obtains hyaluronic acid concentration
Liquid；
Step 4, desolventing technology：The hyaluronic acid concentrate for obtaining is put in organic decolouring system carries out desolventing technology, obtains
Hyaluronic acid clarified solution；
Step 5, ethanol precipitation：The hyaluronic acid clarified solution for obtaining is carried out after PH regulations, adds ethanol to be precipitated；
Step 6, secondary film process：Hyaluronic acid after ethanol precipitation is put in film carries out a chain of filtration, obtains hyaluronic acid
Crude product；
Step 7, secondary precipitation：The hyaluronic acid crude product for obtaining is put into after ethanol precipitated, hyaluronic acid is obtained；
Step 8, drying：The hyaluronic acid for obtaining is dried, hyaluronic acid finished product is obtained.
The step one, PH regulations are carried out by sodium hydroxide solution to the zymotic fluid for obtaining.The step 2, by height
Fast centrifuge is processed the zymotic fluid high speed centrifugation after PH process, removes precipitated impurities.The step 3, by reverse osmosis membrane pair
High retention under the physical state of viable bacteria body, while sufficiently isolating the high enzyme downstream clear liquid of clarity, protects downstream film work
The effect of skill, adopts cotton, timber of containing cellulose etc. for raw material, makes acetic acid, three acetic acid through esterification and hydrolysis fine
Dimension element（CA、CTA）, it is reprocessed into reverse osmosis membrane.The step 4, by organic decolouring membranous system desolventing technology is carried out, can
The materials such as dissolubility small molecular protein, salinity, pigment, it is 100-1000 or so that it carries out separating molecular weight cut off, at surface apertures
In nanoscale, using acetic acid, Triafol T（CA、CTA）For organic decolouring membranous system of main material manufacture.The step
Five, the precipitation salt dissolving obtained after ethanol precipitation is for further processing again.The step 6, is carried out by microfiltration membranes
Process, the material of membrane component has cellulose acetate film (CA) to composite membrane (PA), polyamide patent sandwich diaphragm, polysulfones (PS)
Material film, Kynoar (PVDF) material film, and modified polyacrylonitrile（PAN）Material film.The step 8, by drying
The wet hyaluronic acid for obtaining is dried process by device.
The present invention specifically shows and describes the present invention with reference to preferred embodiment, it is clear that the present invention is implemented not
Limited by aforesaid way, as long as employing the various unsubstantialities improvement that method of the present invention design and technical scheme are carried out,
Or it is not improved the design of invention and technical scheme are directly applied to into other occasions, protection scope of the present invention it
It is interior.
Claims (8)
1. a kind of hyaluronan extraction technique, it is characterised in that extraction process step includes：
Step one, regulation PH：Resulting zymotic fluid is carried out into PH regulations so as in impurity precipitated；
Step 2, centrifugal treating：The zymotic fluid adjusted after PH is put into into centrifuge carries out centrifugal treating, obtains the hyalomitome clarified
Acid fermentation liquid；
Step 3, film process：Hyaluronic acid fermentation liquor is put into into reverse osmosis membrane carries out concentration, obtains hyaluronic acid concentration
Liquid；
Step 4, desolventing technology：The hyaluronic acid concentrate for obtaining is put in organic decolouring system carries out desolventing technology, obtains
Hyaluronic acid clarified solution；
Step 5, ethanol precipitation：The hyaluronic acid clarified solution for obtaining is carried out after PH regulations, adds ethanol to be precipitated；
Step 6, secondary film process：Hyaluronic acid after ethanol precipitation is put in film carries out a chain of filtration, obtains hyaluronic acid
Crude product；
Step 7, secondary precipitation：The hyaluronic acid crude product for obtaining is put into after ethanol precipitated, hyaluronic acid is obtained；
Step 8, drying：The hyaluronic acid for obtaining is dried, hyaluronic acid finished product is obtained.
2. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step one, by hydrogen
Sodium hydroxide solution carries out PH regulations to the zymotic fluid for obtaining.
3. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 2, by height
Fast centrifuge is processed the zymotic fluid high speed centrifugation after PH process, removes precipitated impurities.
4. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 3, by anti-
Permeable membrane, while sufficiently isolating the high enzyme downstream clear liquid of clarity, is protected to the height retention under the physical state of viable bacteria body
The effect of downstream membrane process, adopts cotton, timber of containing cellulose etc. for raw material, through esterification and hydrolysis make acetic acid,
Triafol T（CA、CTA）, it is reprocessed into reverse osmosis membrane.
5. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 4, by having
Machine decolouring membranous system carries out desolventing technology, and by materials such as soluble small molecular protein, salinity, pigments, it carries out separating cutting point
Son amount is 100-1000 or so, and surface apertures are in nanoscale, using acetic acid, Triafol T（CA、CTA）For main material
Organic decolouring membranous system of manufacture.
6. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 5, by second
The precipitation salt dissolving obtained after alcohol precipitation is for further processing again.
7. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 6, by micro-
Filter membrane is processed, and the material of membrane component has cellulose acetate film (CA) to composite membrane (PA), polyamide patent sandwich diaphragm,
Polysulfones (PS) material film, Kynoar (PVDF) material film, and modified polyacrylonitrile（PAN）Material film.
8. a kind of hyaluronan extraction technique according to claims 1, it is characterised in that the step 8, by dry
The wet hyaluronic acid for obtaining is dried process by dry device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845404.XA CN106632727A (en) | 2016-09-24 | 2016-09-24 | A hyaluronic acid extraction process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845404.XA CN106632727A (en) | 2016-09-24 | 2016-09-24 | A hyaluronic acid extraction process |
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| CN106632727A true CN106632727A (en) | 2017-05-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383238A (en) * | 2017-08-20 | 2017-11-24 | 合肥信达膜科技有限公司 | A kind of high yield hyaluronic acid preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008062998A1 (en) * | 2006-11-23 | 2008-05-29 | Lg Life Sciences Ltd. | Method for purifying hyaluronic acid |
| CN101914169A (en) * | 2008-12-01 | 2010-12-15 | 思德生物科技股份有限公司 | Process for purifying hyaluronic acid |
| CN104119456A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院过程工程研究所 | Method for separation and purification of edible (medicinal) mushroom active components by multistage membrane process |
-
2016
- 2016-09-24 CN CN201610845404.XA patent/CN106632727A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008062998A1 (en) * | 2006-11-23 | 2008-05-29 | Lg Life Sciences Ltd. | Method for purifying hyaluronic acid |
| CN101914169A (en) * | 2008-12-01 | 2010-12-15 | 思德生物科技股份有限公司 | Process for purifying hyaluronic acid |
| CN104119456A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院过程工程研究所 | Method for separation and purification of edible (medicinal) mushroom active components by multistage membrane process |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383238A (en) * | 2017-08-20 | 2017-11-24 | 合肥信达膜科技有限公司 | A kind of high yield hyaluronic acid preparation method |
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Application publication date: 20170510 |