CN106632653A - Culture method of reactivation agent having reactivation effects for damaged cells - Google Patents
Culture method of reactivation agent having reactivation effects for damaged cells Download PDFInfo
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- CN106632653A CN106632653A CN201710013074.2A CN201710013074A CN106632653A CN 106632653 A CN106632653 A CN 106632653A CN 201710013074 A CN201710013074 A CN 201710013074A CN 106632653 A CN106632653 A CN 106632653A
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- reactivation
- raw material
- resurrection
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- cultural method
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- 230000007420 reactivation Effects 0.000 title claims abstract description 17
- 230000000694 effects Effects 0.000 title abstract description 4
- 238000012136 culture method Methods 0.000 title abstract 3
- 239000002994 raw material Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 239000006096 absorbing agent Substances 0.000 claims abstract description 8
- 238000012545 processing Methods 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 17
- 102000009024 Epidermal Growth Factor Human genes 0.000 claims description 7
- 101800003838 Epidermal growth factor Proteins 0.000 claims description 6
- 229940116977 epidermal growth factor Drugs 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims description 6
- 108010025020 Nerve Growth Factor Proteins 0.000 claims description 4
- 102000015336 Nerve Growth Factor Human genes 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 229940053128 nerve growth factor Drugs 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 3
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 206010052428 Wound Diseases 0.000 abstract description 5
- 208000027418 Wounds and injury Diseases 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 5
- 210000005036 nerve Anatomy 0.000 abstract description 5
- 230000029663 wound healing Effects 0.000 abstract description 5
- 206010029113 Neovascularisation Diseases 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract 5
- 239000003795 chemical substances by application Substances 0.000 abstract 4
- 238000010521 absorption reaction Methods 0.000 abstract 3
- 230000017423 tissue regeneration Effects 0.000 abstract 1
- 238000002604 ultrasonography Methods 0.000 description 2
- 102000006747 Transforming Growth Factor alpha Human genes 0.000 description 1
- 108010009583 Transforming Growth Factors Proteins 0.000 description 1
- 102000009618 Transforming Growth Factors Human genes 0.000 description 1
- 101800004564 Transforming growth factor alpha Proteins 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/48—Nerve growth factor [NGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present method discloses a culture method of a reactivation agent having reactivation effects for damaged cells. The culture method comprises the following steps: step 1, raw materials are put into a raw material tank; step 2, the raw materials enter into a material mixing tank to conduct a mixing via a material inlet pipe; step 3, the mixed raw materials enter into a heating tank to conduct heating; step 4, the materials are heated into a set temperature, the heated materials enter into an absorber via a material conveying pipe, and absorption liquid in the absorber absorbs the raw material components; step 5, the absorption liquid enters into a reactor; step 6, the absorption liquid enters into a distiller; step 7, an agent preparing machine is used to conduct reaction and processing; and step 8, the finished products of the reactivation agent are obtained. The reactivation agent accelerates the repair and reactivation of damaged cells, and can promote neovascularization, nerve growth, wound tissue repair reaction and wound healing.
Description
Technical field
The present invention relates to a kind of cultural method, more particularly to a kind of resurrection element for making damaged cell that there is On Reactivation
Cultural method.
Background technology
The thermostimulation of the light such as ultrasound knife can cause damaging cell, ultrasound knife mainly to allow ultrasonic energy to be mapped to target every sky
With, and form point-like damage.The existing biological element that brings back to life can only improve ability and the effect of human autoimmune's cell, it is impossible to
Accelerate damaged cell reparation and resurrection；Neovascularization can not be promoted, do not promote nerve growth；Repairing for wound tissue is not promoted
Multiple reaction, does not promote Wound healing.
The content of the invention
The technical problem to be solved is to provide a kind of training of the resurrection element for making damaged cell have On Reactivation
Foster method, it accelerates damaged cell reparation and resurrection；Neovascularization can be promoted, promote nerve growth；Promote wound tissue
Reparation reaction, promote Wound healing.
The present invention is to solve above-mentioned technical problem by following technical proposals：One kind has damaged cell and brings back to life effect
The cultural method of the resurrection element of fruit, it is comprised the following steps：
Step one, raw material raw material tank is put into；
Step 2, raw material is mixed by feed pipe into mixing tank；
Step 3, mixed raw material is heated into heating tank；
Step 4, is heated to after design temperature entering absorber by conveying pipeline, and the absorbing liquid inside absorber absorbs raw material
Composition；
Step 5, absorbing liquid enters reactor；
Step 6, into distiller；
Step 7, the plain device of system is reacted, is processed；
Step 8, obtains bringing back to life plain finished product.
Preferably, the step 5 is comprised the following steps：
Step 5 11, stirring rod is stirred continuously, and absorbing liquid is well mixed；
Step 5 12, persistently stirs, and by heating to inside reactor liquid disinfection.
Preferably, the step 6 is comprised the following steps：
Step 6 11, distiller heating, isolates the impurity in absorbing liquid；
Step 6 12, condenser sprays steam and annular seal space is lowered the temperature by spray head；
Step 6 13, obtains high-purity distillate, flows into the plain device of system.
Preferably, the step 7 is comprised the following steps：
Step 7 11, high-purity distillate flows into the plain device of system；
Step 7 12, beneficial microbe, enzyme in high-purity distillate and the plain device of system, bacterial classification, trace element are mixed to form resurrection
Plain raw material；
Step 7 13, to bringing back to life plain Raw material processing.
Preferably, the resurrection element in the step 8 includes that bringing back to life element is divided into epidermal growth factor subclass, fibroblast life
The long factor, nerve growth factor.
The present invention positive effect be：The present invention accelerates damaged cell reparation and resurrection；New blood vessel can be promoted
Formed, promote nerve growth；Promote the reparation reaction of wound tissue, promote Wound healing.
Description of the drawings
Fig. 1 is that the present invention makes damaged cell have the flow chart of the cultural method of the resurrection element of On Reactivation.
Specific embodiment
Present pre-ferred embodiments are given below in conjunction with the accompanying drawings, to describe technical scheme in detail.
As shown in figure 1, the present invention comprises the following steps the cultural method that damaged cell has the resurrection element of On Reactivation：
Step one, raw material raw material tank is put into；
Step 2, raw material is mixed by feed pipe into mixing tank；
Step 3, mixed raw material is heated into heating tank；
Step 4, is heated to after design temperature entering absorber by conveying pipeline, and the absorbing liquid inside absorber absorbs raw material
Composition；
Step 5, absorbing liquid enters reactor；
Step 6, into distiller；
Step 7, the plain device of system is reacted, is processed；
Step 8, obtains bringing back to life plain finished product.
Step 5 is comprised the following steps：
Step 5 11, stirring rod is stirred continuously, and absorbing liquid is well mixed；
Step 5 12, persistently stirs, and by heating to inside reactor liquid disinfection.
Step 6 is comprised the following steps：
Step 6 11, distiller heating, isolates the impurity in absorbing liquid；
Step 6 12, condenser sprays steam and annular seal space is lowered the temperature by spray head；
Step 6 13, obtains high-purity distillate, flows into the plain device of system.
Step 7 is comprised the following steps：
Step 7 11, high-purity distillate flows into the plain device of system；
Step 7 12, beneficial microbe, enzyme in high-purity distillate and the plain device of system, bacterial classification, trace element are mixed to form resurrection
Plain raw material；
Step 7 13, to bringing back to life plain Raw material processing.
Resurrection element in step 8 includes that bringing back to life element is divided into epidermal growth factor subclass, fibroblast growth factor, nerve
Growth factor.Epidermal growth factor subclass：Accelerate damaged cell reparation and resurrection；Epidermal growth factor subclass includes epidermal growth factor
Son：EGF；TGF：TGF-α and TGF β.Fibroblast growth factor：Neovascularization can be promoted, infringement is repaired
Endothelial cell nerve growth factor：Promote nerve growth；Promote the reparation reaction of wound tissue, promote Wound healing.
Particular embodiments described above, the technical problem, technical scheme and beneficial effect to the solution of the present invention is carried out
Further describe, should be understood that the specific embodiment that the foregoing is only of the invention, be not limited to
The present invention, all any modification, equivalent substitution and improvements within the spirit and principles in the present invention, done etc., should be included in this
Within the protection domain of invention.
Claims (5)
1. it is a kind of to make damaged cell that there is the plain cultural method of the resurrection of On Reactivation, it is characterised in that it is comprised the following steps：
Step one, raw material raw material tank is put into；
Step 2, raw material is mixed by feed pipe into mixing tank；
Step 3, mixed raw material is heated into heating tank；
Step 4, is heated to after design temperature entering absorber by conveying pipeline, and the absorbing liquid inside absorber absorbs raw material
Composition；
Step 5, absorbing liquid enters reactor；
Step 6, into distiller；
Step 7, the plain device of system is reacted, is processed；
Step 8, obtains bringing back to life plain finished product.
2. make damaged cell that there is the cultural method of the resurrection element of On Reactivation as claimed in claim 1, it is characterised in that institute
State step 5 to comprise the following steps：
Step 5 11, stirring rod is stirred continuously, and absorbing liquid is well mixed；
Step 5 12, persistently stirs, and by heating to inside reactor liquid disinfection.
3. make damaged cell that there is the cultural method of the resurrection element of On Reactivation as claimed in claim 1, it is characterised in that institute
State step 6 to comprise the following steps：
Step 6 11, distiller heating, isolates the impurity in absorbing liquid；
Step 6 12, condenser sprays steam and annular seal space is lowered the temperature by spray head；
Step 6 13, obtains high-purity distillate, flows into the plain device of system.
4. make damaged cell that there is the cultural method of the resurrection element of On Reactivation as claimed in claim 1, it is characterised in that institute
State step 7 to comprise the following steps：
Step 7 11, high-purity distillate flows into the plain device of system；
Step 7 12, beneficial microbe, enzyme in high-purity distillate and the plain device of system, bacterial classification, trace element are mixed to form resurrection
Plain raw material；
Step 7 13, to bringing back to life plain Raw material processing.
5. make damaged cell that there is the cultural method of the resurrection element of On Reactivation as claimed in claim 1, it is characterised in that institute
Stating the element of the resurrection in step 8 includes that bringing back to life element is divided into epidermal growth factor subclass, fibroblast growth factor, nerve growth factor
Son.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013074.2A CN106632653A (en) | 2017-01-09 | 2017-01-09 | Culture method of reactivation agent having reactivation effects for damaged cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013074.2A CN106632653A (en) | 2017-01-09 | 2017-01-09 | Culture method of reactivation agent having reactivation effects for damaged cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106632653A true CN106632653A (en) | 2017-05-10 |
Family
ID=58843506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710013074.2A Pending CN106632653A (en) | 2017-01-09 | 2017-01-09 | Culture method of reactivation agent having reactivation effects for damaged cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106632653A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958793A (en) * | 2006-11-23 | 2007-05-09 | 西安组织工程工程技术研究中心 | Method for preparing composite growth factor of natural cells |
| CN203021487U (en) * | 2012-11-28 | 2013-06-26 | 海南巨鹿堂保健美容品有限公司 | Natural bioactive polypeptide and cell factor complex liquid preparation device |
| CN103405751A (en) * | 2013-08-22 | 2013-11-27 | 赵轩 | Composition with cell repairing function and preparation method and application thereof |
| CN104622709A (en) * | 2015-01-30 | 2015-05-20 | 姜振宇 | Human stem cell factor skin repairing solution and preparation method thereof |
| CN105457102A (en) * | 2014-09-05 | 2016-04-06 | 陕西艾美雅生物科技有限公司 | Purifying and concentrating method for composite bioactive factors and concentrated solution |
-
2017
- 2017-01-09 CN CN201710013074.2A patent/CN106632653A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958793A (en) * | 2006-11-23 | 2007-05-09 | 西安组织工程工程技术研究中心 | Method for preparing composite growth factor of natural cells |
| CN203021487U (en) * | 2012-11-28 | 2013-06-26 | 海南巨鹿堂保健美容品有限公司 | Natural bioactive polypeptide and cell factor complex liquid preparation device |
| CN103405751A (en) * | 2013-08-22 | 2013-11-27 | 赵轩 | Composition with cell repairing function and preparation method and application thereof |
| CN105457102A (en) * | 2014-09-05 | 2016-04-06 | 陕西艾美雅生物科技有限公司 | Purifying and concentrating method for composite bioactive factors and concentrated solution |
| CN104622709A (en) * | 2015-01-30 | 2015-05-20 | 姜振宇 | Human stem cell factor skin repairing solution and preparation method thereof |
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Application publication date: 20170510 |