CN106617106A

CN106617106A - Nutritional supplement capable of delaying senescence

Info

Publication number: CN106617106A
Application number: CN201611177806.3A
Authority: CN
Inventors: 常宸
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-12-19
Filing date: 2016-12-19
Publication date: 2017-05-10

Abstract

The invention relates to a nutritional supplement capable of delaying senescence. The active ingredients of the supplement include 80-20wt% of tobacco protein and 20-80wt% of echinacea polysaccharide. Compared with a traditional nutritional supplement, the nutritional supplement provided by the invention has the advantages of being simple in preparation method and reasonable in compatibility, and two active ingredients can work together to improve the ageing speed of a body while developing respective efficacies.

Description

A kind of nutritious supplementary pharmaceutical of anti-aging

Technical field

The present invention relates to food and field of health care products, and in particular to a kind of nutritious supplementary pharmaceutical.

Background technology

With the raising of living standards of the people, the public is increasingly strong to nutrition and the requirement of health, containing various in food Nutrient, but because species is different, its distribution is also differed with content.Additionally, in the production, processing and preserving process in food, Nutrient often suffers a loss.For the deficiency of nutrient in supplement, nutritive value of food is improved, adapt to different crowd Need, nutrition fortifier can be added.Nutrition fortifier has simplified meals concurrently and processes, conveniently ingests and disease-prevention health etc. is acted on.It is special Not current, food nutrient fortifying has become the important means that many national governments improve its commonwealth general level of the health.

Presently commercially available nutritious supplementary pharmaceutical mostly is inorganic molecules compound, and these compounds are mainly obtained by chemical synthesis .Researcher constantly looks for originating natural, effect significantly, particularly the nutritious supplementary pharmaceutical with delaying senility function.

In tobacco leaf numerous compositions, enriched the most with protein content.Protein in tobacco leaf be divided into soluble protein and Insoluble protein.Half or so is chloroplast protein in soluble protein（Fraction I protein, FI albumen）, separately Half is the compound of other soluble proteins（Fraction II protein, F II albumen）.In the past people were mostly Concentrate on the protein content for improving crop fructification, less focus on the exploitation of plant leaf protein.With And leaf protein research is deepened continuously, people gradually recognize that plant leaf protein is protein resource most abundant in the world, are Solve a new food proteins source of our times population increasingly expansion issues.

Some results of study show that plant leaf protein is especially high with soluble protein content in tobacco leaf, in FI albumen Various essential amino acids contents are not only above essential amino acids content standard in the protein product of the World Food Programme's formulation, its Middle tyrosine, phenylalanine, threonine and leucine are above 1 times or so of the standard, than some staple food crops such as paddy rice, Essential amino acids content in wheat, corn and soybean protein is all high, shows that FI albumen has higher nutriture value in tobacco leaf Value.Tobacco leaf protein matter is more suitable for absorption of human body and utilizes than egg, cheese and milk, with higher nutritive value and medicinal valency Value.

The FI albumen that early stage research worker extracts is often with color and taste, and complex manufacturing, and investment is big, produces Amount is low, and people are difficult to receive.Later, many researchers were purified to the FI albumen in tobacco leaf, had obtained high-purity FI albumen, the progressively simplification of wherein Kung and Tso, Lowe to tobacco leaf FI protein purification methods is made that very big contribution.So And, these extracting methods have hypervelocity or at a high speed freezing step with centrifugal separation, and mostly process is both needed to control at 0-4 DEG C or so； Also have and FI albumen is extracted using methods such as isoelectric focusing, gel electrophoresis, ultrafiltration.Although these methods can obtain pure FI eggs In vain, but relatively costly, the yield that the unit interval extracts is relatively low, it is difficult to realize large-scale industrial production.The extraction skill of FII albumen Art is simpler than FI albumen a lot, and further investigation has been also carried out both at home and abroad, and has had been enter into the application stage, and such as tobacco leaf selenoprotein is made For food additives and health food in China's field of food extensive application.

Tobacco leaf protein in addition to the basic function characteristic with protein, also with many special functional characteristics.Tobacco leaf Selenium is rich in albumen, is existed with selenomethionine and selenocystein or other forms.Selenium has extensive medical care effect, mends The pre- anti-cancer of selenium energy, hepatitis, angiocardiopathy etc. are filled, selenium prevents leukopenic effect to the chemotherapy of cancer patient.So And, selenium is again the trace element of nutrition dosage and toxicity dose narrow range, therefore, selenium of the development with the efficient attribute of low toxicity is produced Product are significant with therapy-related disease for preventing.Using metabolism of the tobacco leaf to inorganic selenium, protein state selenium is obtained；Nothing During protein state selenium is converted into, toxicity is substantially reduced machine selenium, is mainly shown as declining to a great extent for acute toxicity.It is existing Ample evidence shows, compared with sodium selenite, tobacco leaf selenoprotein has low toxicity high-efficient characteristic, thus tobacco leaf selenoprotein is used as new Selenium source has potential advantages.At present, tobacco leaf selenoprotein is by the approval of the Ministry of Public Health of China as food additives and with immunity tune Section, the health food of function of delaying senility.The combustion product of tobacco leaf protein has a negative impact to the suction taste of cigarette, thus from cigarette Tobacco leaf protein is extracted in leaf, remaining part is used for into production of cigarettes, will be the developing direction of tobacco comprehensive utilization.

Tobacco leaf protein obtains various biologically active peptides Jing after enzyme hydrolysis.These peptides can be small enough to only 2 ammonia The dipeptides of base acid, or the long-chain or ring type polypeptide of complexity, and be mostly through glycosylation, phosphorylated derivatives products Thing.Modern biotechnology metabolism research finds：The protein of human consumption after gastral various enzyme hydrolysis, unlike having previously been thought that As absorbed only in the form of amino acid, be more directly to be absorbed in the form of low peptide, and dipeptides and tripeptides Infiltration rate than same composition amino acid faster.Recent studies indicate that：Biologically active peptide is a class multifunctional agents, raw The growing of thing, cell differentiation, brain activity, neoplastic lesion, immune defense, reproduction control, anti-aging, biological clock rule and Molecular evolution etc. all refers to biologically active peptide.A class bioactive micro peptide such as antibacterial active peptide, typically have antibacterium or The effect of fungi, some also have the function of antiprotozoan, virus or tumour cell.Antibacterial peptide has the antibacterial action of wide spectrum, right Gram-positive bacteria, Gram-negative bacteria, fungi are respectively provided with inhibitory action；The tumour cell in animal body can also be killed, but The normal cell not destroyed in animal body.And for example blood pressure lowering peptide, is have the one of notable hypotensive activity after native protein hydrolysis Class peptide, Long-Time Service also has no toxic side effect.Blood pressure lowering peptide is mainly by the angiotensin-converter in Reverse transcriptase human body Enzyme activity and reach hypotensive purpose.The tridecanoic peptide extracted from tobacco leaf protein, presses down with antibacterial activity and protease System activity, and be a kind of potential protease inhibitors with higher stability.

At present, it is special about being rarely reported by the research country that raw material produces biologically active peptide and amino acid of tobacco protein It is not to prepare biologically active peptide aspect, foreign countries also seldom report.And say from food and the angle of nutrition, with biologically active peptide as work( The energy factor, can develop low antigen food, baby food, sports food, rush calcium uptake food, blood pressure-reducing food, sobering food Etc. a series of products.

Plant physiologist thinks, tobacco leaf protein it is best in quality, many containing amino acid classes, be most easily absorbed by the body profit With；And per 667m²Well-grown tobacco, can extract out the protein of 35kg colorless and odorless.Tobacco protein both can be diluted to Albumin, can be processed into cake, margarine and the cows milk substitute of various delicious foods again, such as select halogen, become again delicate Tobacco leaf bean curd.In ice conditions, soft cream is can be made into, compared with animality cream in the market, this cream has Two big prominent advantages：1. it is many containing unsaturated fat；2. quantity of heat production is low, and more food will not be fat.In addition, tobacco protein also has growing Ease constipation stomach and the effects such as stimulate circulation.

Using interior circumscribed albumen complex enzyme, tobacco leaf protein is carried out into Controlled-enzymatic Hydrolysis process, according to the difference of its Degree of Enzymatic Hydrolysis, A series of biologically active peptides and amino acid can be obtained.Using classification membrane separation technique, classification UF membrane and macroporous resin adsorption Separation or ion exchange resin separation GC-MS are isolated and purified, enrichment method target product, then the corresponding working processes of Jing, can be made Obtain a series of functional foods；Be there is into Maillard reactions in the enzymolysis liquid of discarded tobacco leaf and reduced sugar, alcohol etc., can synthesize and have Strong fruital, nut are fragrant, bake the cigarette that perfume, paste flavor, Chinese herbal medicine are fragrant or tobacco is fragrant uses and flavorant, and these spices can improve cigarette The aroma quality of silk, increases perfume quantity, mitigates the miscellaneous gas and excitant of cigarette, makes smoke alcohol and full, and can coordinate cigarette perfume, improves Pleasant impression, for the perfuming of high, middle-grade cigarette, can strengthen the fine quality of high, middle-grade cigarette.

The cDNA of glycinin is transferred to into tobacco gene group Agrobacterium-mediated In transformation, glycinin is expressed in tobacco seed, and is occurred in a kind of special mode.With it is big Globulin in beans is compared, and resulting transgene tobacco glycinin is in protein expression level, processing characteristics, active work( Can be with no significant difference on accumulating level.

Test data shows, tobacco be for producing medical treatment and scientific research in terms of collagen recombinant product it is most valuable The raw material of value.Collagen accounts for the 30% of its total protein in mammalian body, and its main component is fibrillar collagen matter I-III, this A little collagens constitute the crossed fiber network structure of extracellular matrix albumen, have very important effect in human body.Will Tobacco is used to produce collagen restructuring series of products, can not only solve the raw material difficult problem that collagen product is produced in medicine and pharmacology, also Be conducive to the raising of medical technique level, promote the further development of medical industry.

Research finds there is special cDNAs in the nectary of tobacco.CDNAs can encode out special plant rennet, this The function that protease has opposing microorganism infringement is planted, so as to protect whole plant from infringement.How sharp currently study With transgenic technology, carry out this special cDNAs transgenosis and process, realization carries out corresponding gene table in other plant Reach, then this plant can just resist corresponding microorganism infringement, strengthen the defencive function of itself, so as to reduce the use of agricultural chemicals Amount, reduces the residues of pesticides of agricultural product, while also mitigating the pollution to natural environment.

Echinacea（Echinacea）, also known as genus echinacea or Echinacea, it is perennial composite family draft flowering plant, Originate in central North America and east.At present, the category has found altogether 9 kinds and 2 mutation, wherein 3 kinds have medical value, i.e., Echinacea（Echinacea purpurea）, narrow leaf Echinacea（Echinacea augustifolia）And white Echinacea （Echinacen pallida）.Echinacea is distributed widely in the U.S., Canada and Europe.In recent years, China successfully introduces a fine variety purple cone Chrysanthemum, has plantation on Shaanxi, Henan, Hunan and other places.The plant is used to treating wound, toothache, snake and stings by iroquois people earliest Wound and septicopyemia etc., are then used to prevent and treat the illnesss such as upper respiratory disease, skin disease and anaphylactia at present.Echinacea has There are various efficient bio-actives such as immunological regulation, anti-inflammatory, anti-oxidant, sterilized, antiviral and anticancer, thus receive much concern.

Immunoregulatory activity is that Echinacea is most important, the biologically active for also attracting most attention, including following several respects：（1） Promote the growth and development of immune organ.Research discovery, Echinacea Purpurea Herb P.E（Echinacea purpurea extract, EE） The quality of mouse spleen can be significantly improved.According to the literature, compound Echinacea can significantly improve chicken bursa of Fabricius, thymus gland and spleen Dirty index, promotes Development of Immune Organs.Test proves that EE significantly improves the Immune Organs Index of AA Broiler chicks.（2）Improve blood Immunologic function and biochemical indicator.EE can not only improve mice serum immunoglobulin level and reduce total cholesterol level, And can improve the red blood cell RBG-C of broiler chicken₃BR rosette rates and serum proleulzin and TNF Contents, additionally it is possible to Improve routine blood indexes.Echinacea compound can improve total protein and albuminous content in Lingnanhuang broilers blood plasma, reduce sweet Oily three ester levels.（3）Adjust the function and propagation of immunocyte.EE can either remarkably promote normal mouse marrow and spleen nature Cell and monocytic propagation are killed, also increases the quantity of leukemia mouse spleen NK cells, be also remarkably improved broiler chicken NK The killing ability of cell.Research finds that EE is capable of the propagation of obvious stimulation T lymphocyte, and Cytokines in Peripheral Blood Mononuclear Propagation also have facilitation.EE can activating macrophage, strengthen its phagocytic activity and stimulate its produce IL-1, IL-10 and The cell factors such as TNF-a and nitric oxide.（4）Strengthen humoral immunity.Research shows that EE can significantly improve broiler chicken peripheral blood IBDV and NDV antibody titer, and compound Echinacea also has significantly enhancing to the NDV antibody titers of sea blue Baigong chicken Effect.（5）Other.Many acids in Echinacea also have preventive protection to act on stress in rats gastric ulcer, and may It is relevant with raising immunity of organisms and enhancing NO synzyme vigor.Echinacea can also improve the word material conversion ratio of livestock and poultry, so as to change Kind production performance.

It was discovered by researchers that the polysaccharide component of narrow leaf Echinacea has anti-inflammatory activity, it can suppress carrageenan to induce Mouse podedema and croton oil induction mice auricle swelling.It is reported that, a kind of isobutylamides in Echinacea- Dodecatetraenoic acid, can play antiinflammatory action by suppressing LOX.Proved by vitro test, in EE Alkylamide material can suppress cyclooxygenase and 5- LOXs.EE can also suppress mouse macrophage prostaglandin The generation of E2.Cichoric acid is considered as the material that can most suppress hyaluronidase in Echinacea.And hyaluronidase can be hydrolyzed Bright matter acid and chondroitin, promote proinflammatory factor to pass through matrix, expand inflammatory reaction.Research shows that the scorching effect of suppression of Echinacea is also It is relevant with suppressing huge saliva cell to produce NO and TNF-a.And further study and point out, Echinacea is to the inhibitory action that NO synthesizes By improving arginase activities, and induced NOS is suppressed to express.

Document report, three kinds of medicinal Echinaceas（Echinacea purpurea、Echinacea augustifolia、 Echinacen pallida）Root extract various free radicals are respectively provided with Scavenging activity, and people's low-density lipoprotein can be suppressed White oxidation.Confirm that above-mentioned three kinds medicinal Echinacea leaves and root are extracted by radicals scavenging experiment and lipid peroxidation experiment The oxidation resistance of thing.Research finds that the caffeic acid derivative in EE has a good antioxidation, and with EE other into When point being used in combination, antioxidant effect is higher.Result of study shows, EE samples prepared by three kinds of methods（Many acid contents are respectively 3.76%、6.40%、33.56%）It is respectively provided with antioxidation, and their reducing power and to H₂O₂Scavenging action be better than Vitamin C.EE not only itself has certain antioxidation, and can strengthen the oxidation resistance of animal body.Research Show, EE can strengthen the oxidation resistance of mouse and AA Broiler chicks.

Research finds that the polysaccharide component in Echinacea cells and supernatant can strengthen cytophagous function, so as to Play a protective role during mouse infection Candida albicans and Listeria monocytogenes.Scientific research personnel has found that Echinacea is just Oneself burns extract and has antifungal activity, can suppress the growth of brewer's yeast and various candida albicans.Research shows, genus echinacea Root extract is to Cryptococcus neoformans, Candida albicans and various pathogenic filamentous fungis, such as trichophyta, beard and hair hair addiction The little robe bacterium of bacterium, gypsum sample, Pseudoallescheria boydii have antifungal activity.In addition to above-mentioned fungi, EE is also to other various causes Sick bacterium, such as micrococcus scarlatinae, haemophilus influenzae, legionella pneumophilia, clostridium difficile and propionibacterium acnes, golden yellow Portugal Grape coccus, mycobacterium smegmatis are inhibited.

It is reported that, EE can increase leukemia mouse spleen NK cell quantities and extend its life-span.The anticancer of Echinacea is made With mainly related to NK cells into close：On the one hand, the alkylamide material of Echinacea can pass through to suppress 5-LOX's and COX Produce, the generation of PG is lowered, so as to promote the propagation of NK cells；On the other hand, the Arabic gala in Echinacea polysaccharide component Glycan induces the cell factors such as its secretion IL-1, INF- α, IFN-β, so as to strengthen the thin of NK cells by activating macrophage Born of the same parents kill ability.EE is not only able to wave antitumaous effect by NK iuntercellular sending and receiving, and itself also being capable of direct killing cancer cell.Examination Issue after examination and approval now, the hexane extract of echinacea root is equal to human pancreas cancer MIA PACA-2 clones and colon cancer COLO320 clone There is lethal effect.

Research shows that the various biologically actives of Echinacea are based on its own different chemical composition.At present, EE is by liquid phase color The active component that spectrum mass spectrometric hyphenated technique is identified, mainly there is alkylamide compound and the big class of caffeic acid derivative two：Before Person includes 12 carbon -2E, 4E, 8Z, 10E/Z- tetraenoic acid isobutyramide, 11 carbon -2E, 4Z- diene -8,10- diacetylenic acid isobutyls Acid amides, 11 carbon -2Z, 4E- diene -8,10- diacetylenic acid isobutyramides, 11 carbon -2E, 4Z- diene -8,10- diacetylenic acid -2- first Base butyramide etc.；The latter includes Cichoric acid, monocaffeyltartaric acid, chlorogenic acid, coffee sigh acid etc., and the polysaccharide in EE is mainly by mountain Pears sugar, mannose, arabinose, rhamnose, galactolipin, 6 kinds of monose compositions of wood sugar.

It is isolated with immunocompetent two kinds of polysaccharide, PS from the aerial part of Echinacea Echinacea purpurea I and PS II, its structure is respectively：4-O- methyl-grape alditol-arabinose-xylan glycan（Mean molecule quantity is 35000）With acid arabinose-sandlwood galactolipin glycan（Mean molecule quantity is 50000）.Due to extracting pure from Echinacea Change polysaccharide is costly and is difficult to obtain, therefore someone is separated with tissue culture technique from tissue culture medium, obtains 2 points The acidic polysaccharose that son amount is respectively 10000,25000 neutral polysaccharide and 1 molecular weight is 75000, obtains from tissue cultures Polysaccharide be mainly the primary wall composition of cultured cells, therefore different from polysaccharide detached from plant, its activity is also variant.

At present, there is not been reported for the application for combining with Echinacea polysaccharide for nutritional supplementation by tobacco protein.

The content of the invention

The technical problem to be solved is to provide one kind can effectively delay body aging, have no toxic side effect, activity The natural nutritious supplementary pharmaceutical of ingredient origin.

The present invention the adopted technical scheme that solves the above problems is to provide a kind of nutritious supplementary pharmaceutical of anti-aging, mends Fill active component in agent to be made up of tobacco protein and Echinacea polysaccharide, the percentage by weight that wherein tobacco protein accounts for active component is 80-20%, it is 20-80% that Echinacea polysaccharide accounts for the percentage by weight of active component.

Preferably, the percentage by weight that tobacco protein accounts for active component is 80-50%, and Echinacea polysaccharide accounts for active component Percentage by weight is 20-50%.

It is furthermore preferred that the percentage by weight that tobacco protein and Echinacea polysaccharide account for active component is respectively 75% and 25%.

Preferably, the tobacco protein is tobacco FI albumen（Fraction I protein）.

Preferably, tobacco FI albumen of the invention（Fraction I protein）Prepared using following methods：In removing The Nicotiana tabacum leaves of arteries and veins, add NaCl solution, anion exchange resin and thin base ethanol, are smashed to pieces at a high speed with tissue mashing machine, green Homogenate filtered through gauze.By filtrate in 50 DEG C of insulations, then cool down rapidly, discard precipitation.Supernatant passes through DEAE- celluloses Post, with trichloroacetic acid protein is detected, and collects albumen part.Tris-Cl buffer solutions are added to the albumen part collected, in 4 DEG C place 4-6 days after separate out mass crystallization, obtain final product.

Preferably, Echinacea polysaccharide of the invention is prepared using following methods：The Echinacea herb raw material of ultramicro grinding is taken, Add water and boil, extract clear liquid；Concentrate after activated carbon decolorizing, then use ethanol precipitating；Precipitating thing is centrifuged to obtain；By the drying of precipitating thing, grind Grind, sieve, obtain Thick many candies；Thick many candies add water and are sufficiently stirred for, and filter at 4 DEG C, and supernatant is taken after centrifugation；Add Sevage reagents Removing protein is removed, upper liquid is concentrated, with ethanol precipitation polysaccharide is separated out, Jing centrifugations obtain precipitating thing；Precipitating thing is washed with ethanol, very Sky is dried, grinding, obtains the material of white powder.

Preferably, the composition further includes acceptable additive on food or health products.

It is furthermore preferred that the one kind of acceptable additive in preservative, thickener and flavor enhancement etc. on the food Or it is various.

Most preferably, the preservative is benzoic acid；Thickener is pectin or gelatin, preferably pectin；Flavor enhancement be Ah This Ba Tian.

The present invention also provides a kind of application of above-mentioned nutritious supplementary pharmaceutical in the food or health products for preparing anti-aging.

The invention has the advantages that：It is surprising that through repeated tests, present inventors have unexpectedly found that Tobacco protein and Echinacea polysaccharide are applied in combination the effect for having Synergistic for body anti-aging.Mend with conventional nutraceutical Fill agent to compare, the replenishers preparation method of the present invention is simple, rationally, two kinds of active components is playing the same of respective effect to compatibility When, and can cooperate with and improve body aging speed.

Specific embodiment

The present invention is further described with reference to embodiment, but embodiments of the present invention not limited to this.

Preparation example 1, tobacco FI albumen（Fraction I protein）Preparation

Take 130 grams of Nicotiana tabacum leaves for going middle arteries, add the NaCl solution of 130g 0.1M, 30 gram of 711 anion exchange resin and 1ml dredges base ethanol, is smashed to pieces half a minute at a high speed with tissue mashing machine, and green homogenate is with four layers of filtered through gauze.By filtrate in 50 DEG C Insulation 10 minutes, then cools down rapidly, discards precipitation.By DEAE- cellulose column DE53, flow velocity is 1.0ml/min/ to supernatant cm³.Protein is detected with trichloroacetic acid, the albumen part for flowing out is collected.Equal-volume 0.025M is added to the albumen part collected Tris-Cl, pH 7.4 includes the buffer solution of 0.2mM EDTA.Mass crystallization is separated out after placing 6 days in 4 DEG C, is obtained final product.Jing converts, About 7mg/ gram fresh weight leaf of crystal yields.

The preparation of preparation example 2, Echinacea polysaccharide

The Echinacea herb raw material of ultramicro grinding is taken, adds the water of 20 times of amounts, heating to boil 3 hours, filter impurity, extract clear Liquid；Jing activated carbon decolorizings again；Liquid is concentrated after decolouring, then uses ethanol precipitating；Precipitating thing is obtained using centrifugal method；Again will Precipitating thing is dried, ground, crosses 60 mesh sieves, obtains Thick many candies；Thick many candies add water and are sufficiently stirred for, and filter at 4 DEG C, take after centrifugation Clear liquid；Add Sevage reagents to remove removing protein, upper liquid is concentrated, with 5 times of amount ethanol precipitations polysaccharide is separated out, Jing centrifugations are sunk Analysis thing；Washed with ethanol again 2 times, be vacuum dried, grinding obtains the material of white powder.

Experimental technique used in following embodiments 1-5 and test example 1 if no special instructions, is conventional method, makes Raw material tobacco protein and Echinacea polysaccharide are respectively tobacco FI albumen and Echinacea polysaccharide obtained in preparation example 1 and 2.

Embodiment 1

The tobacco protein of 3 weight portions and the Echinacea polysaccharide of 1 weight portion are weighed, is poured into after both are mixed in mixer and is stirred 10 Minute, dispense as requested, it is packaged into packed, obtain final product anti-aging nutritious supplementary pharmaceutical.

Embodiment 2

The tobacco protein of 30 weight portions and the Echinacea polysaccharide of 10 weight portions are weighed, it is bright with 50 weight portions after both are mixed The benzoic acid of glue, the Aspartame of 5 weight portions and 5 weight portions is together poured in mixer and stirred 15 minutes, is dispensed as requested, It is packaged into packed, obtains final product the nutritious supplementary pharmaceutical of anti-aging.

Embodiment 3

The tobacco protein of 10 weight portions and the Echinacea polysaccharide of 30 weight portions are weighed, with the fruit of 50 weight portions after both are mixed The benzoic acid of glue, the Aspartame of 5 weight portions and 5 weight portions is together poured in mixer and stirred 15 minutes, is dispensed as requested, It is packaged into packed, obtains final product the nutritious supplementary pharmaceutical of anti-aging.

Embodiment 4

Weigh the tobacco protein of 8 weight portions and the Echinacea polysaccharide of 32 weight portions, after both are mixed with the gelatin of 50 weight portions, The benzoic acid of the Aspartame of 5 weight portions and 5 weight portions is together poured in mixer and stirred 15 minutes, is dispensed as requested, is wrapped Dress up packed, obtain final product the nutritious supplementary pharmaceutical of anti-aging.

Embodiment 5

Weigh the tobacco protein of 32 weight portions and the Echinacea polysaccharide of 8 weight portions, after both are mixed with the pectin of 50 weight portions, The benzoic acid of the Aspartame of 5 weight portions and 5 weight portions is together poured in mixer and stirred 15 minutes, is dispensed as requested, is wrapped Dress up packed, obtain final product the nutritious supplementary pharmaceutical of anti-aging.

The application of test example 1, the nutritious supplementary pharmaceutical containing tobacco protein/Echinacea polysaccharide in mice age is delayed

1. animal packet and administration

70 mouse are divided into at random 7 groups：Blank group, model group and medicine group A1-A5 of the present invention.Standard feed point cage is raised Support, free diet drinking-water is tested after raising 1 week.Medicine group A1-A5 is given with the normal saline solution gavage of active component Medicine, the accumulated dose of active component is 1.0g/kg, wherein A1 groups gavage 1.0g/kg tobacco protein, and A2 groups gavage 1.0g/kg is purple to bore Chrysanthemum polysaccharide, the tobacco protein and 0.8g/kg Echinacea polysaccharide of A3 group gavages 0.2g/kg, the tobacco protein of A4 group gavages 0.5g/kg With 0.5g/kg Echinacea polysaccharide, the tobacco protein and 0.25g/kg Echinacea polysaccharide, blank group and mould of A5 group gavages 0.75g/kg Type group equal-volume normal saline solution gastric infusion；Simultaneously model group and medicine group A1-A5 press 100mgkg for administration^-1Modeling Dosage nape part hypodermic injection D- galactolipins, continuous 6 weeks., with equal-volume physiological saline, gavage is with injection simultaneously for blank group Carry out, testing fatigue is carried out after continuous 42d.

2. mouse fatigue experiment and result

After mouse stomach modeling 6 weeks, transfer rod instrument fatigue experiment is carried out, mouse is placed on into transfer rod（Transfer rod diameter 30mm, length 60mm）On, rotating speed is 10rmin^-1, every mouse is respectively trained 5min so as to adapt to the rotation in transfer rod.Next day, by mouse It is placed on transfer rod, rotating speed is adjusted to 30rmin^-1, the time of staying of the accurate recording mouse on transfer rod, maximum duration is limited to 10min, more than 10min 10min is then designated as, front with 70% ethanol transfer rod and miscellaneous part per batch test.

Compare with blank group, D- galactolipin modeling mouse agings substantially shorten in the transfer rod time of staying, and medicine group A1- A5 can increase mouse aging muscle power, wherein A5 groups best results, and the difference between each experimental group is statistically significant, tool Body the results are shown in Table 1.

Impact of the nutritious supplementary pharmaceutical of the present invention of table 1 to mouse time of staying on transfer rod fatigue instrument

3. mouse fatigue related biochemical indicator is determined and result

Administration weighs Mouse Weight after terminating, and carries out eye socket blood sampling, 4000rmin^-1It is to be measured serum to be collected after centrifugation 10min. The method that the Biochemical Indexes such as BUN, MDA, NO, NOS are provided using related kit in serum, in strict accordance with kit Illustrate to be operated, determine each biochemical indicator numerical value.

Compare with blank group, BUN, MDA, NO and NOS content is dramatically increased in D- galactolipin modeling mouse aging serum, and Medicine group A1-A5 significantly reduces above-mentioned biochemical indicator content, wherein A5 groups best results, and specific experiment the results are shown in Table 2.

The nutritious supplementary pharmaceutical of the present invention of table 2 is to the tired impact with anti-oxidant related biochemical indicator in mice serum

4. conclusion

As a result prove, pharmaceutical composition of the present invention can reduce the content of BUN, MDA, NO and NOS in mice serum, pointing out should Pharmaceutical composition can improve the oxidation resistance of body, remove cylinder super-oxidation lipid, reduce D- galactolipins and cause aging course In elevated NO and NOS contents, increase caused cytotoxicity so as to mitigate NO, play a part of anti-aging.

Particularly in the case where active component total amount keeps constant, using the present invention containing tobacco protein and Echinacea polysaccharide Nutritious supplementary pharmaceutical it is bright compared with the replenishers containing tobacco protein or Echinacea polysaccharide, each biochemical indicator content of mice plasma is used alone It is aobvious to reduce, it was demonstrated that tobacco protein and being applied in combination for Echinacea polysaccharide generate synergy, reduce the use of active component Amount.Wherein tobacco protein and many sugar weight ratios of Echinacea are 3:1 nutritious supplementary pharmaceutical effect is projected the most, is generated and is difficult to be expected Excellent effect.

Claims

1. a kind of nutritious supplementary pharmaceutical of anti-aging, it is characterised in that the active component of anti-aging is by tobacco egg in replenishers It is 80-20% that white and Echinacea polysaccharide composition, wherein tobacco protein account for the percentage by weight of active component, and Echinacea polysaccharide accounts for work Property composition percentage by weight be 20-80%.

2. nutritious supplementary pharmaceutical according to claim 1, it is characterised in that tobacco protein accounts for the percentage by weight of active component For 80-50%, it is 20-50% that Echinacea polysaccharide accounts for the percentage by weight of active component.

3. nutritious supplementary pharmaceutical according to claim 2, it is characterised in that tobacco protein and Echinacea polysaccharide account for active component Percentage by weight be respectively 75% and 25%.

4. nutritious supplementary pharmaceutical according to claim 1, it is characterised in that the nutritious supplementary pharmaceutical further includes food Or acceptable additive on health products.

5. nutritious supplementary pharmaceutical according to claim 4, it is characterised in that acceptable additive is selected from anti-on the food One or more in rotten agent, thickener and flavor enhancement etc..

6. nutritious supplementary pharmaceutical according to claim 5, it is characterised in that the preservative is benzoic acid；Thickener is fruit Glue or gelatin, preferably pectin；Flavor enhancement is Aspartame.

7. application of the nutritious supplementary pharmaceutical described in any one of claim 1-6 in the food or health products for preparing anti-aging.