CN106615071A - Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii - Google Patents
Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii Download PDFInfo
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- CN106615071A CN106615071A CN201611183149.3A CN201611183149A CN106615071A CN 106615071 A CN106615071 A CN 106615071A CN 201611183149 A CN201611183149 A CN 201611183149A CN 106615071 A CN106615071 A CN 106615071A
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- botrytis cinerea
- pterostilbene
- peronophythora
- peronophythora litchii
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- VLEUZFDZJKSGMX-UHFFFAOYSA-N pterostilbene Natural products COC1=CC(OC)=CC(C=CC=2C=CC(O)=CC=2)=C1 VLEUZFDZJKSGMX-UHFFFAOYSA-N 0.000 title claims abstract description 71
- VLEUZFDZJKSGMX-ONEGZZNKSA-N pterostilbene Chemical compound COC1=CC(OC)=CC(\C=C\C=2C=CC(O)=CC=2)=C1 VLEUZFDZJKSGMX-ONEGZZNKSA-N 0.000 title claims abstract description 71
- 241000123650 Botrytis cinerea Species 0.000 title claims abstract description 64
- 241001443921 Phytophthora litchii Species 0.000 title claims abstract description 59
- 230000002265 prevention Effects 0.000 title abstract description 6
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 75
- 241000233614 Phytophthora Species 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims description 22
- 238000003306 harvesting Methods 0.000 claims description 21
- 208000015181 infectious disease Diseases 0.000 claims description 21
- 201000010099 disease Diseases 0.000 claims description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 18
- 244000183278 Nephelium litchi Species 0.000 claims description 17
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 17
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 17
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 9
- 230000004763 spore germination Effects 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 230000003902 lesion Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000009467 reduction Effects 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 6
- 241000219094 Vitaceae Species 0.000 abstract description 5
- 235000021021 grapes Nutrition 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 241001629511 Litchi Species 0.000 abstract 2
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 241000219095 Vitis Species 0.000 description 16
- 238000000034 method Methods 0.000 description 13
- 239000000725 suspension Substances 0.000 description 9
- 244000052769 pathogen Species 0.000 description 8
- 230000003449 preventive effect Effects 0.000 description 8
- 241000582441 Peronospora tabacina Species 0.000 description 7
- 230000001717 pathogenic effect Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 239000012452 mother liquor Substances 0.000 description 6
- 239000001965 potato dextrose agar Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000003385 bacteriostatic effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 240000000560 Citrus x paradisi Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 3
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 3
- 235000021286 stilbenes Nutrition 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000004913 activation Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 235000009984 Pterocarpus indicus Nutrition 0.000 description 1
- 244000086363 Pterocarpus indicus Species 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 240000008424 Vaccinium ashei Species 0.000 description 1
- 235000013468 Vaccinium ashei Nutrition 0.000 description 1
- 235000019631 acid taste sensations Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229940064004 antiseptic throat preparations Drugs 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.
Description
Technical field
The present invention relates to biological technical field, more particularly to pterostilbene is in preventing and treating grape grey mould and peronophythora litchi
Application.
Background technology
Grape and lichee are important industrial crops, it has also become the pillar of many rural economic developments, but two kinds of fruits
All it is difficult preservation and freshness, invents new antistaling agent or develop new preservation technique and increased after the adopting of two fruits with controlling its postharvest disease
Value and extend the supply phase on will play vital effect.
Grape grey mould is one of most important disease after grape harvest, and pathogen is Botrytis cinerea (Botrytis
Cinerea), grape fruit rot, threshing during sale and low temperature storing and transporting can be caused.By Peronophythora Litchii germ
The peronospora tabacina that (Peronophythora litchi Chen ex Ko et al.) causes be lichee postharvest storage it is fresh-keeping in most
Main disease, under conditions of humiture is suitable, germ infects rapidly extension, causes the brown stain of pericarp, pulp to rot and ooze
Go out the juice of vinic acid taste, seriously limit the post harvest transport of fruit.
At present to the control of postharvest diseases of fruit mainly based on chemical agent, but there is improper use and cause residues of pesticides
Jeopardize human health and be easily caused the risk that pathogen develops immunity to drugs.Therefore, select to people, animal and beneficial organism safety, it is right
The stress-free natural products control postharvest disease of ecological environment becomes one of study hotspot in recent years.
Pterostilbene (Pterostilbene:3,5- dimethoxy-4 's '-hydroxy diphenyl ethylene) it is that a kind of important plant is more
Phenol, in being present in the plants such as narra, vaccinium ashei and peanut.
The content of the invention
In order to overcome shortcoming of the synthetic organic pesticide on preventing and treating postharvest diseases of fruit and deficiency, it is an object of the invention to
A kind of natural plants metabolite pterostilbene is provided in terms of fruit postharvest diseases grape grey mould and peronophythora litchi preventing and treating
New application.
It is an object of the present invention to provide the following purposes of pterostilbene or its solution：
The invention provides pterostilbene or its solution suppress Peronophythora Litchii germ (Peronophythora litchii)
In application.
The invention provides pterostilbene or its solution suppress Peronophythora Litchii germ (Peronophythora litchii)
Application in mycelial growth and/or sporangium sprouting.
The invention provides pterostilbene or its solution are in preventing and treating peronophythora litchi (Peronophythora litchii)
In application；
Or pterostilbene or its solution are in preventing and treating post-harvest fruits infection Peronophythora Litchii germ (Peronophythora
Litchii the application in).
In above-mentioned application, preventing and treating post-harvest fruits infection Peronophythora Litchii germ (Peronophythora litchii)
It is embodied in reduction post-harvest fruits infection Peronophythora Litchii germ disease index and/or browning index；
Or the fruit is lichee.
The invention provides pterostilbene or its solution answering in Botrytis cinerea (Botrytis cinerea) is suppressed
With.
The invention provides pterostilbene or its solution are suppressing the life of Botrytis cinerea (Botrytis cinerea) mycelia
Application in long and/or spore germination.
The invention provides pterostilbene or its solution answering in preventing and treating Botrytis cinerea (Botrytis cinerea)
With；
Or, application of the pterostilbene in preventing and treating post-harvest fruits infection Botrytis cinerea (Botrytis cinerea).
In above-mentioned application, preventing and treating post-harvest fruits infection Botrytis cinerea (Botrytis cinerea) is embodied in drop
The low post-harvest fruits infection Botrytis cinerea incidence of disease and/or lesion diameter；
Or the fruit is grape.
Another object of the present invention is to provide a kind of product.
The product that the present invention is provided, its active component is pterostilbene or pterostilbene solution；
The product has following 1) -8) at least one of function：
1) Peronophythora Litchii germ (Peronophythora litchii) is suppressed；
2) Peronophythora Litchii germ (Peronophythora litchii) mycelial growth and/or sporangium is suppressed to sprout；
3) peronophythora litchi (Peronophythora litchii) is prevented and treated；
4) post-harvest fruits infection Peronophythora Litchii germ (Peronophythora litchii) are prevented and treated；
5) Botrytis cinerea (Botrytis cinerea) is suppressed；
6) Botrytis cinerea (Botrytis cinerea) mycelial growth and/or spore germination are suppressed；
7) Botrytis cinerea (Botrytis cinerea) is prevented and treated；
8) post-harvest fruits infection Botrytis cinerea (Botrytis cinerea) are prevented and treated.
In above-mentioned, the solute of the pterostilbene solution is pterostilbene, and it by concentration is that 50.0mmol/L, pH value are that solvent is
7.4 phosphate buffer and the NaOH aqueous solution that concentration is 1.0mol/L, pH value is 13 are 9 according to volume ratio:1 is mixed to get
Mixed liquor.
By to Botrytis cinerea (Botrytis cinerea) and Peronophythora Litchii germ (Perononphythora
Litchii In Vitro Bacteriostatic experiment) finds that pterostilbene has significant bacteriostatic activity to two kinds of pathogens, additionally by purple
Wingceltis stilbene substantially reduce be inoculated with after adopting pathogen grape and litchi fruits rot experiment confirm pterostilbene to grape grey mould and
The preventive effect of peronophythora litchi, it is achieved thereby that the purpose of the present invention.
When actually used, in order that pterostilbene can be very good adhesion in grape or lichee surface, pterostilbene solution can be with
Add some fresh-keeping conventional adhesives.
The experiment proves that, the pterostilbene material of the present invention can be used for two kinds of post-harvest grapes grey mold on grape and lichee
Germ (Botrytis cinerea) and the preventing and treating of Peronophythora Litchii germ (Perononphythora litchii) disease, both
Can be used alone, it is also possible to be used in combination with other antiseptics, to strengthen antibacterial effect or reduce its toxic and side effect, green,
Safety, is beneficial to health, meets food safety requirements, serves and prevents and treats gray mold and peronospora tabacina and to the fresh-keeping of fruit
Effect.
Description of the drawings
Fig. 1 is pterostilbene to Botrytis cinerea (Botrytis cinerea) and Peronophythora Litchii germ
The inhibitory action of (Peronophythora litchii) mycelial growth.
Fig. 2 is prevention effect of the pterostilbene to post-harvest grapes fruit gray mold；
A processes fruit and is inoculated with germ without pterostilbene；B is processed with 100 μ g/mL pterostilbenes and be inoculated with germ after fruit；C is used
250 μ g/mL pterostilbenes to process and be inoculated with germ after fruit；D is processed with 500 μ g/mL pterostilbenes and be inoculated with germ after fruit；1000 μ of E
G/mL pterostilbenes to process and be inoculated with germ after fruit；F is processed with 10000 μ g/mL pterostilbenes and be inoculated with germ after fruit.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, commercially obtain.
Embodiment 1, pterostilbene is to Botrytis cinerea and the bacteriostatic activity of Peronophythora Litchii germ
Detected with mycelial growth rate method：
For trying pathogen：Botrytis cinerea Botrytis cinerea separate the grape fruit of idiopathy
(Rosslenbroich H,Stuebler D.Botrytis cinerea-history of chemical control and
novel fungicides for its management[J].Crop Protection.2000,19:557-561), lichee
Peronospora tabacina bacterium Peronophythora litchii (Cai Xueqing, Lin Na, Chen Wei, etc. the biological characteristics of Peronophythora Litchii
[J]. tropical crops journal, 2009,30 (9):1226-1231) separate the litchi fruits of idiopathy.
Main agents：Pterostilbene is purchased from lark prestige Science and Technology Ltd., purity ＞ 98%.
Potato dextrose agar PDA is used to cultivate Botrytis cinerea, and radish juice agar medium CA is used
In culture Peronophythora Litchii germ.
Inhibitory action of the variable concentrations pterostilbene to pathogen mycelial growth, concrete behaviour are determined with mycelial growth rate method
Make：(1) with phosphate buffer (PBS；50.0mmol/L, PH7.4, formula：If preparing 100mL, 80.0mL50.0mmol/L is taken
Na2HPO4With 20.0mL 50.0mmol/L KH2P04Mix)：(volume ratio is the NaOH aqueous solution (1.0mol/L, PH13)
9:1) it is configured to mother liquor as solvent dissolving pterostilbene；(2) a certain amount of prepared pterostilbene mother liquor is accurately added respectively to melting
It is down to after change in 50 DEG C of PDA or CA culture mediums and mixes so that the final concentration of pterostilbene respectively reaches 25,50,100 in culture medium
With 200 μ g/mL, each concentration is processed and arranges 3 repetitions, to add the solvent of same volume as control, with the amount of 15mL/ wares
Pour diameter 9cm culture dishes into；(3) with sterilization punchers on PDA the colony edge of the Botrytis cinerea of activation culture 5d
Or the colony edge of the Peronophythora Litchii germ of activation culture 5d beats the consistent bacteria cake of cell age (d=6mm) on CA, and by bacteria cake
Reversal connection enters the central authorities of the culture medium flat plate containing upper variable concentrations pterostilbene, is placed in 22 DEG C (Botrytis cinereas) or 25 DEG C of (lichee
Peronospora tabacina bacterium) cultivate in constant incubator；(4) routine observation record experimental result.
As a result Fig. 1 is seen, pterostilbene has significant fungistatic effect, works as purple to Botrytis cinerea and Peronophythora Litchii germ
When wingceltis stilbene concentration reaches 200 μ g/mL, 57.9% He is respectively reached to the bacteriostasis rate of Botrytis cinerea and Peronophythora Litchii germ
70.1%.
Embodiment 2, pterostilbene is to Botrytis cinerea and the bacteriostatic activity of Peronophythora Litchii germ
Spore germination method：
The preparation of pathogen spore/sporangia suspension：Respectively by Botrytis cinerea and Peronophythora Litchii germ in PDA
Or 5d is cultivated on CA culture mediums, and then add sterilized water 5mL to be rinsed and vibrate in flat board, prepare spore/sporangium and hang
Floating mother liquor.Potato dextrose broth (PDB) is added in Botrytis cinerea spore suspension mother liquor, after being diluted
Botrytis cinerea spore suspension；Sterilized water is added in Peronophythora Litchii germ sporangia suspension, litchi after being diluted
Branch peronospora tabacina bacterium sporangia suspension；Spore/the sporangia suspension of dilution pathogen to concentration is 105Individual/mL.
The concrete operations that spore germination method is determined：(1) with phosphate buffer (PBS；50.0mmol/L,PH7.4)：NaOH
(volume ratio is 9 to the aqueous solution (1.0mol/L, PH13):1) it is configured to mother liquor as solvent dissolving pterostilbene；(2) accurately add respectively
Enter and fully mixed in spore/sporangia suspension of the pterostilbene mother liquor of a certain amount of preparation to after diluting so that be purple in spore liquid
The final concentration of wingceltis stilbene respectively reaches 10,20,30 and 40 μ g/mL, to add the solvent of same volume as control；(3) will everywhere
Reason spore liquid is placed under conditions of 22 DEG C (Botrytis cinereas) or 25 DEG C (Peronophythora Litchii germ) and cultivates；(4) routine observation
Spore/sporangial sprouting situation, each concentration is processed and arranges 3 repetitions, 3 area of visual field of each repeat count altogether about 100
Individual spore/sporangium, counts spore/sporangial germination rate.
Result of the test is shown in Table 1：
The inhibitory action that the pterostilbene of table 1 sprouts to Botrytis cinerea spore and Peronophythora Litchii germ sporangium
The result of table 1 shows that pterostilbene is to Botrytis cinerea spore and the suppression of Peronophythora Litchii germ sporangium sprouting
Effect apparently higher than control group, to grape grey mold under the pterostilbene concentration of 10 μ g/mL, 20 μ g/mL, 30 μ g/mL and 40 μ g/mL
The inhibiting rate of germ spore germination respectively reaches 20.4%, 43.4%, 93.3% and 100%, to Peronophythora Litchii germ spore
The inhibiting rate that capsule is sprouted reaches 70.4%, 100%, 100% and 100%, and extremely strong bacteriostatic activity is presented.
Embodiment 3, pterostilbene is tested to the preventive effect of post-harvest grapes gray mold (B.cinerea)
Material to be tested：For trying grape variety " mare's milk ", purchased from market.Simple grain grape fruit is manually separated, base of fruit is left,
Choose the consistent healthy fruit of size, shape, maturity to sterilize in the aqueous sodium hypochlorite solution of weight/mass percentage composition 2%
2min, then with aseptic water washing 2 times, dries rear packet transaction.
Concrete operations：(1) depth 2mm, the wound of a diameter of 1.5mm are pierced out in fruit equatorial positions with aseptic inoculation pin
Mouthful；(2) it is respectively connected to the pterostilbene that 10 μ L concentration are 100,250,500,1000,10000 μ g/mL in the wound of each fruit
Solution (phosphate buffer (PBS；50.0mmol/L,PH7.4)：(volume ratio is 9 to the NaOH aqueous solution (1.0mol/L, PH13):
1) as solvent), each concentration is processed and arranges 3 repetitions, and 60 fruits are inoculated with altogether, to access the solvent of same volume as right
According to；(3) room temperature is placed 30min and is dried after fruit, and it is 5 × 10 to be inoculated with 10 μ L concentration in the wound of every fruit4The Portugal of individual/mL
Grape ash arrhizus bacteria spore suspension (compound method is with embodiment 2)；(4) fruit of each concentration process is respectively placed in into plastics fresh-keeping
In box, spraying and moisturizing is placed in 22 DEG C of storages；(5) preserve after 6d and 9d, observe and record the incidence of disease and lesion diameter of fruit.
Experimental result is as shown in table 2 and Fig. 2：
Prevention effect of the pterostilbene of table 2 to post-harvest grapes fruit gray mold
Test result indicate that：Pterostilbene has good preventive effect to grape grey mould, and as the rising preventive effect of concentration is improved.
When for trying concentration being 500 μ g/mL, preserve and 36.12% is reached to the inhibiting rate of the fruit incidence of disease after 9d, the suppression to lesion diameter
Effect is up to 51%.The generation of grape grey mould can be completely inhibited when the concentration of pterostilbene is 10000 μ g/mL.
Embodiment 4, pterostilbene adopts the live body preventive effect experiment of rear peronospora tabacina (P.litchii) to lichee
Material to be tested：For trying Litchi Varieties " Huaihe River branch ", Conghua City of Guangdong Province is picked up from, transported back immediately at laboratory after adopting
Reason.Clip fruit simultaneously retains base of fruit, chooses the consistent healthy fruit of size, maturity and is tested.
Concrete operations：(1) surface sterilization 2min is carried out to litchi fruits with 2% liquor natrii hypochloritis, is rushed with sterilized water
Wash 2 times, dry rear packet transaction；(2) pterostilbene that litchi fruits are soaked in respectively 200,400,800 and 1600 μ g/mL is molten
In liquid, process taking-up room temperature after 10min and dry naturally, each concentration is processed and arranges 3 repetitions, 60 fruits is inoculated with altogether, with phase
The solvent of same volume processes fruit as control；(3) it is 2 × 10 that the equatorial positions in lichee pericarp are inoculated with 10 μ L concentration4Individual/mL
White phytophthora sporangia suspension (compound method is with embodiment 2)；(4) fruit is respectively put into spraying and moisturizing in crisper, is put
Cultivate at 25 DEG C；(5) observe and record the disease index and browning index of fruit.
Fruit morbidity rating calculation standard be：0 without substantially infection；1 infection area for fruit≤5%；3 infection areas
For the 6%-15% of fruit；5 infection areas are the 16%-25% of fruit；7 infection areas are the 26%-49% of fruit；9 infection
Area for fruit >=50%.Disease index=Σ (morbidity grade × corresponding fruit number)/(9 × fruit total quantity) × 100.
The standard of fruit browning rating calculation is：1 without brown stain；2 slight brown stains；3 browned areas are the ＜ 1/4 of fruit；4 is brown
Variable area is the 1/4-1/2 of fruit；5 infection areas are the ＞ 1/2 of fruit.Browning index=Σ (morbidity grade × corresponding fruit
Quantity)/fruit total quantity.
Experimental result is as shown in table 3：
The pterostilbene of table 3 adopts the prevention effect of rear peronospora tabacina to lichee
| Pterostilbene concentration (μ g/mL) | Disease index | Browning index |
| Control | 64.26 | 3.12 |
| 200 | 55.56 | 2.75 |
| 400 | 42.22 | 2.60 |
| 800 | 33.33 | 2.53 |
| 1600 | 28.15 | 2.20 |
Test result indicate that：Pterostilbene has preferable preventive effect to peronophythora litchi, and with the rising of pterostilbene concentration
Preventive effect is lifted.After storage 6d, the fruit entirety disease index of control treatment is up to 64.26, and pterostilbene concentration is 200,400,800
During with 1600 μ g/mL, 13.54%, 34.30%, 48.13% and 56.19% is respectively reached to the preventive effect of litchi fruits morbidity.Separately
Outward, pterostilbene can slow down the brown stain of pericarp, extending fruit it is fresh-keeping, improve its quality and (include in above MATERIALS METHODS
The division of brown stain grade, if reducing the brown stain of lichee pericarp in theory, improves to a certain extent the fresh-keeping and product of fruit
Matter).
Claims (10)
1. the application of pterostilbene or its solution in Peronophythora Litchii germ (Peronophythora litchii) is suppressed.
2. pterostilbene or its solution suppress Peronophythora Litchii germ (Peronophythora litchii) mycelial growth and/or
Or the application in sporangium sprouting.
3. the application of pterostilbene or its solution in preventing and treating peronophythora litchi (Peronophythora litchii).
4. pterostilbene or its solution are in preventing and treating post-harvest fruits infection Peronophythora Litchii germ (Peronophythora litchii)
In application.
5. application according to claim 4, it is characterised in that：
Preventing and treating post-harvest fruits infection Peronophythora Litchii germ (Peronophythora litchii) is embodied in after reduction adopts
Fruit infects Peronophythora Litchii germ disease index and/or browning index；
Or the fruit is lichee.
6. the application of pterostilbene or its solution in Botrytis cinerea (Botrytis cinerea) is suppressed；
Or pterostilbene or its solution are suppressing Botrytis cinerea (Botrytis cinerea) mycelial growth and/or spore germination
In application.
7. the application of pterostilbene or its solution in preventing and treating Botrytis cinerea (Botrytis cinerea)；
Or, application of the pterostilbene in preventing and treating post-harvest fruits infection Botrytis cinerea (Botrytis cinerea).
8. application according to claim 7, it is characterised in that：
Preventing and treating post-harvest fruits infection Botrytis cinerea (Botrytis cinerea i) is embodied in reduction post-harvest fruits sense
The dye Botrytis cinerea incidence of disease and/or lesion diameter；
Or the fruit is grape.
9. a kind of product, its active component is pterostilbene or pterostilbene solution；
The product has following 1) -8) at least one of function：
1) Peronophythora Litchii germ (Peronophythora litchii) is suppressed；
2) Peronophythora Litchii germ (Peronophythora litchii) mycelial growth and/or sporangium is suppressed to sprout；
3) peronophythora litchi (Peronophythora litchii) is prevented and treated；
4) post-harvest fruits infection Peronophythora Litchii germ (Peronophythora litchii) are prevented and treated；
5) Botrytis cinerea (Botrytis cinerea) is suppressed；
6) Botrytis cinerea (Botrytis cinerea) mycelial growth and/or spore germination are suppressed；
7) Botrytis cinerea (Botrytis cinerea) is prevented and treated；
8) post-harvest fruits infection Botrytis cinerea (Botrytis cinerea) are prevented and treated.
10. according to arbitrary described application in claim 1-8 or the product described in claim 9, it is characterised in that：
The solute of the pterostilbene solution is pterostilbene, and it by concentration is that 50.0mmol/L, the phosphate that pH value is 7.4 delay that solvent is
It is 9 according to volume ratio that liquid and concentration are rushed for 1.0mol/L, the NaOH aqueous solution that pH value is 13:1 mixed liquor being mixed to get.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107714678A (en) * | 2017-10-23 | 2018-02-23 | 吉林大学 | Application of the pterostilbene in the enzyme inhibitors of MCR 1 are prepared |
| CN109699866A (en) * | 2018-12-30 | 2019-05-03 | 陕西师范大学 | The pterostilbene application that Aspergillus carbonerius growth and ochratoxin A generate in inhibiting grape juice |
| CN112616911A (en) * | 2020-12-08 | 2021-04-09 | 陕西师范大学 | Application of pterostilbene in delaying fruit senescence and preventing and treating apple and pear tiger skin diseases |
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| WO2007042557A2 (en) * | 2005-10-13 | 2007-04-19 | Universite Victor Segalen - Bordeaux 2 | Composition for phytopharmaceutical application to stimulate natural controls of plants |
| CN102120996A (en) * | 2010-12-03 | 2011-07-13 | 西北农林科技大学 | Method for generating pterostilbene by utilizing grape resveratrol-oxygen-methyl transferase to catalyze resveratrol |
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| WO2007042557A2 (en) * | 2005-10-13 | 2007-04-19 | Universite Victor Segalen - Bordeaux 2 | Composition for phytopharmaceutical application to stimulate natural controls of plants |
| CN102120996A (en) * | 2010-12-03 | 2011-07-13 | 西北农林科技大学 | Method for generating pterostilbene by utilizing grape resveratrol-oxygen-methyl transferase to catalyze resveratrol |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107714678A (en) * | 2017-10-23 | 2018-02-23 | 吉林大学 | Application of the pterostilbene in the enzyme inhibitors of MCR 1 are prepared |
| CN107714678B (en) * | 2017-10-23 | 2020-06-02 | 吉林大学 | Application of pterostilbene in preparation of MCR-1 enzyme inhibitor |
| CN109699866A (en) * | 2018-12-30 | 2019-05-03 | 陕西师范大学 | The pterostilbene application that Aspergillus carbonerius growth and ochratoxin A generate in inhibiting grape juice |
| CN109699866B (en) * | 2018-12-30 | 2022-03-18 | 陕西师范大学 | Application of pterostilbene in inhibiting growth of aspergillus carbonarius and generation of ochratoxin A in grape juice |
| CN112616911A (en) * | 2020-12-08 | 2021-04-09 | 陕西师范大学 | Application of pterostilbene in delaying fruit senescence and preventing and treating apple and pear tiger skin diseases |
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| CN106615071B (en) | 2020-12-08 |
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