CN106610377A - Seed spectral detection method and system - Google Patents
Seed spectral detection method and system Download PDFInfo
- Publication number
- CN106610377A CN106610377A CN201611000759.5A CN201611000759A CN106610377A CN 106610377 A CN106610377 A CN 106610377A CN 201611000759 A CN201611000759 A CN 201611000759A CN 106610377 A CN106610377 A CN 106610377A
- Authority
- CN
- China
- Prior art keywords
- seed
- fluorescence
- cylinder
- reflected light
- light source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a seed spectral detection method and system. The method comprises a black box and a processing device. A seed bearing station, a light emitting diode LED light source unit, a spectrometer and a power source are arranged in the black box. The power source is electrically connected to the LED light source unit. The LED light source unit emits monochromatic lights to the seed to be detected on the seed bearing station to excite the seed to generate reflected light and fluorescence. The spectrometer collects the reflected light and the fluorescence and acquires the reflected spectral data and fluorescence spectral data. The spectrometer is electrically connected to the processing device. The spectrometer transmits the reflected light spectral data and the fluorescence spectral data to the processing device. The seed spectral detection method and system can realize nondestructive detection of the seeds to be detected.
Description
Technical field
The present invention relates to agricultural technology, more particularly to a kind of seed spectral method of detection and system.
Background technology
Seed inspection refers to a kind of utilization science, advanced and standard method, and the quality of seed sample is detected, divided
Analysis, identification, to judge the technology of its quality good or not.Wherein, the index that Seed inspection is detected mainly includes cleanliness, purity, life
Vigor, vigor, Maturity, damage etc..
At present, to the detection of seed except conventional using germination percentage, germinating energy, the measure of vitality index and raw body dyeing etc.
Outside method, there are some special survey methods, example in fields such as cytology, morphology, physicss, chemistry, molecular biology
Such as:Fluorogen method, hypertonic sproutung method, chemical method, seed coat structure microscopy etc. on hollow measure, paper.But, using it is above-mentioned this
During a little methods are detected to seed, all seed can be damaged, it is impossible to demand when meeting actually used.Example
Such as:When detecting to seed using chemical method, the chemical reaction produced by chemical reagent and seed is needed to obtain kind
The testing result of son so that the seed destruction after being contacted by chemical formula reagent.
Therefore, how to realize to the Non-Destructive Testing of seed it being a problem demanding prompt solution.
The content of the invention
The present invention provides a kind of seed spectral method of detection and system, to solve prior art in how to realize to seed
Non-Destructive Testing technical problem.
First aspect present invention provides a kind of seed spectral detection system, including:Camera bellows and processing equipment;
Include in the camera bellows:Seed plummer, LED light source cell, spectrogrph, power supply;
The power supply is electrically connected with the LED light source unit;
The LED light source unit to the seed to be detected on the seed plummer sends monochromatic light, to excite the kind
Son produces reflected light and fluorescence;
Reflected light described in the spectrometer collection and the fluorescence, obtain reflected light spectroscopic data and fluorescence data;
The spectrogrph is electrically connected with the processing equipment, and the spectrogrph is by the reflected light spectroscopic data and described glimmering
Light spectroscopic data is transferred to the processing equipment.
Optionally, also include in the camera bellows:Optical filter and optical fiber;
The optical filter is connected by the optical fiber with the spectrogrph;
Reflected light and fluorescence of the spectrometer collection after optical filter filtration.
Optionally, the quantity of the LED light source unit is one or more.
Optionally, when the quantity of the LED light source unit is multiple, multiple LED light source units are held with the seed
The central shaft of microscope carrier is uniformly distributed for the center of circle.
Optionally, the seed plummer is fixedly mounted on the bottom of the camera bellows by mounting post;
The mounting post includes:First cylinder, the second cylinder, fixture, the bottom of first cylinder is fixed on described
The bottom of camera bellows, and first cylinder is hollow cylinder, the internal diameter of first cylinder is more than the external diameter of second cylinder;
In the movable insertion of second cylinder first cylinder, by the fixture by first cylinder and described
Second cylinder is fixedly connected.
Optionally, also include in the camera bellows:Elastomeric element;
The elastomeric element is set in mounting post outside, and positioned at the bottom of the seed plummer and the camera bellows
Between.
Optionally, the radiator for LED light source unit radiating is additionally provided with the camera bellows.
Second aspect present invention provides a kind of seed spectral method of detection, including:
Monochromatic light is sent to seed to be detected, to excite the seed to produce reflected light and fluorescence;
The reflected light and the fluorescence are gathered, reflected light spectroscopic data and fluorescence data is obtained;
According to the reflected light spectroscopic data and the fluorescence data, the testing result of the seed to be detected is obtained
Seed spectral method of detection and system that the present invention is provided, it is to be detected to excite by sending monochromatic light to seed
Fluorescence and reflected light that seed is produced, such that it is able to get the fluorescence data and reflected light spectrum number of the seed to be detected
According to, and then the testing result that the two spectroscopic datas obtain seed to be detected can be based on.In this way, can not break
In the case of bad seed to be detected, the testing result of seed to be detected is got, realize the Non-Destructive Testing to seed to be detected.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
The structural representation of the seed spectral detection system embodiment one that Fig. 1 is provided for the present invention;
The schematic flow sheet of the seed spectral method of detection embodiment one that Fig. 2 is provided for the present invention.
Description of reference numerals:
1:Camera bellows; 2:Processing equipment;
3:Seed plummer; 4:LED light source unit;
5:Spectrogrph; 6:Power supply;
7:Optical filter; 8:Optical fiber;
9:Mounting post; 10:First cylinder;
11:Second cylinder; 12:Fixture;
13:Elastomeric element.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Seed spectral method of detection and system that the present invention is provided, it is intended to solve how effectively to monitor electricity in prior art
Depending on the technical problem of signal.
Below technical scheme is described in detail with specifically embodiment.These specific enforcements below
Example can be combined with each other, for same or analogous concept or process may be repeated no more in some embodiments.
The structural representation of the seed spectral detection system embodiment one that Fig. 1 is provided for the present invention.As shown in figure 1, this reality
Apply the seed spectral detection system that example provided to can be used for seed to be detected is detected, analyzed, identified, to judge seed
Quality.The seed spectral detection system can include:Set for isolating the camera bellows 1 of the interference of ambient and noise, process
Standby 2;Include in camera bellows 1:Seed plummer 3, light emitting diode (Light Emitting Diode, referred to as:LED) light source cell
4th, spectrogrph 5, power supply 6;
Power supply 6 is electrically connected with LED light source unit 4;LED light source unit 4 to the seed to be detected on seed plummer 3 sends
Monochromatic light, to excite seed to produce reflected light and fluorescence;Spectrogrph 5 gathers reflected light and fluorescence, obtains reflected light spectroscopic data
And fluorescence data;Spectrogrph 5 is electrically connected with processing equipment 2, and spectrogrph 5 is by reflected light spectroscopic data and fluorescence data
It is transferred to processing equipment 2.Wherein, spectrogrph 5 and processing equipment 2 can be connected with civil power or existing electric supply installation, think
Spectrogrph 5 and processing equipment 2 provide the power that can drive spectrogrph 5 and the work of processing equipment 2.
Specifically, above-mentioned LED light source unit 4 can excite seed to be detected to send fluorescence for arbitrary by monochromatic light
LED light source unit 4, such as monochromatic LED etc..Wherein, the power of the LED light source unit 4, centre wavelength, bandwidth, specifically
One or more conditions that can be in the type of seed, the size of camera bellows 1, Testing index etc. determine, for example:The LED light
The wavelength of source unit 4 can be consistent with the excitation wavelength of the fluorescence of seed to be detected.
Optionally, the quantity of above-mentioned LED light source unit 4 can be one, or it is multiple, specifically can be according to seed
Type, the size of camera bellows 1, Testing index etc. in one or more conditions determine.When the quantity of above-mentioned LED light source unit 4
For it is multiple when, the plurality of LED light source unit 4 can be evenly distributed in camera bellows 1 by the center of circle of the central shaft of seed plummer 3,
Can be with according to the demand of user setting installation site etc..It is illustrated that in Fig. 1 so that " two LED light source units 4 are with seed carrying
The central shaft of platform 3 is uniformly distributed for the center of circle " as a example by seed spectral detection system.
Optionally, if when the monochromatic monochromaticity that sent of above-mentioned LED light source unit 4 is poor (for example:LED light source list
Monochromatic broader bandwidth that unit 4 is sent etc.), then it is also provided between above-mentioned LED light source unit 4 and seed to be detected
Narrow band pass filter, so that the LED light source unit 4 can send the preferable monochromatic light of monochromaticity.
Optionally, it is also provided with (not showing in Fig. 1 for the radiator of the radiating of LED light source unit 4 in above-mentioned camera bellows 1
Go out), the heat operationally produced to disperse LED light source unit 4, so that LED light source unit 4 can produce stabilized intensity
Monochromatic light.When implementing, the power of above-mentioned radiator, quantity, and installation site specifically can be according to LED light source unit
4 power, the quantity of LED light source unit 4, size of camera bellows 1 etc. determine.Correspondingly, it is above-mentioned for LED light source unit 4 power supply
Power supply 6 can also be a constant current source 6, to further ensure that the monochromatic stabilized intensity that LED light source unit 4 is sent.
Above-mentioned processing equipment 2 can be arbitrary processing equipment 2 with reception, transmission and processing function, for example:Calculate
Machine, single-chip microcomputer, processor etc..In the present embodiment, the processing equipment 2 can receive the seed to be detected that spectrogrph 5 is sent
Reflected light spectroscopic data and fluorescence data, it is possible at the reflected light spectroscopic data and fluorescence data
Reason, to obtain the testing result of seed to be detected.
The course of work of the seed spectral detection system is simply introduced below, specifically:
First, on the seed plummer 3 seed to be detected being placed in camera bellows 1.Secondly, the power supply 6 in camera bellows 1 is opened
Power for LED light source unit 4, so that LED light source unit 4 can send monochrome to the seed to be detected on seed plummer 3
Light, to excite seed to produce reflected light (monochromatic light that i.e. reflex LED light source unit 4 is sent) and fluorescence (i.e. seed Intradermal
The fluorescence that chlorophyll or red fluorescent protein are sent under the monochromatic irradiation that LED light source unit 4 sends);Then, position
Spectrogrph 5 in camera bellows 1 can gather the reflected light and fluorescence, to get the reflected light spectroscopic data of the seed to be detected
And fluorescence data, and the reflected light spectroscopic data and fluorescence data are sent to into processing equipment 2.Finally, process sets
Standby 2, by the analysis to the reflected light spectroscopic data and fluorescence data, obtain the testing result of seed to be detected.By upper
State mode so that seed spectral detection system can get seed to be detected in the case where seed to be detected is not destroyed
Testing result, realizes the Non-Destructive Testing to seed to be detected.
The seed spectral detection system that the present invention is provided, by LED light source unit 4 fluorescence of seed generation to be detected is excited
And reflected light, so that spectrogrph 5 can get the fluorescence data of the seed to be detected and reflected light spectroscopic data,
And then allow processing equipment 2 that the testing result of seed to be detected is obtained based on the two spectroscopic datas.In this way,
Allow seed spectral detection system in the case where seed to be detected is not destroyed, the detection of seed to be detected can be got
As a result, the Non-Destructive Testing to seed to be detected is realized.
With continued reference to Fig. 1, on the basis of above-described embodiment, can also include in above-mentioned camera bellows 1:Optical filter 7 and optical fiber
8;Wherein, optical filter 7 is connected by optical fiber 8 with spectrogrph 5;Spectrogrph 5 gathers the reflected light and glimmering after optical filter 7 is filtered
Light.
Specifically, in the present embodiment, above-mentioned optical filter 7 is used to weaken the reflected light of seed to be detected, so that spectrogrph 5
The reflected light spectrum and fluorescence spectrum of the seed to be detected for being gathered is in identical rank.So, processing equipment 2 is according to place
In the reflected light spectrum and fluorescence spectrum of same levels, the testing result of the seed to be detected for being drawn is more accurate.Due to this
The monochromatic light that the reflected light of seed to be detected is sent by LED light source unit 4, therefore, the optical filter 7 allows the wavelength model for passing through
Enclose, bandwidth, transmitance, cut-off depth, material etc., the monochromatic wavelength that specifically can be sent according to LED light source unit 4,
And, the practical situation of seed spectral detection system determines.Wherein, above-mentioned optical filter 7 can be narrow band pass filter, light cutoff filter
Piece, notch filtering light piece etc..When above-mentioned optical filter 7 is narrow band pass filter, the centre wavelength of the narrow band pass filter can with it is to be checked
The consistent wavelength of the fluorescence produced by seed is surveyed, is part cut-off at the monochromatic wavelength that LED light source unit 4 is sent
's.When above-mentioned optical filter 7 is edge filter, the edge filter can allow the fluorescence produced by seed to be detected to lead to
Cross, but be part cut-off at the monochromatic wavelength that LED light source unit 4 is sent.When above-mentioned optical filter 7 is notch filtering light
During piece, the notch filtering light piece can only be part cut-off at the monochromatic wavelength sent in LED light source unit 4, but
The light for allowing other wavelength passes through.
The seed spectral detection system that the present invention is provided, is connected by being provided with optical fiber 8 in camera bellows 1 with spectrogrph 5
The optical filter 7 for connecing so that spectrogrph 5 can gather reflected light and fluorescence after optical filter 7 is filtered, so that spectrogrph
The reflected light spectrum and fluorescence spectrum of 5 seeds to be detected for being gathered is in identical rank, and then causes processing equipment 2 in root
According to reflected light spectrum and fluorescence spectrum in same levels, the testing result of the seed to be detected for being drawn is more accurate, carries
The high accuracy rate of the testing result of seed to be detected.
With continued reference to Fig. 1, on the basis of above-described embodiment, above-mentioned seed plummer 3 is fixedly mounted on by mounting post 9
The bottom of camera bellows 1;
Wherein, mounting post 9 includes:First cylinder 10, the second cylinder 11 and fixture 12, the bottom of the first cylinder 10 is fixed
In the bottom of camera bellows 1, and the first cylinder 10 is hollow cylinder, and the internal diameter of the first cylinder 10 is more than the external diameter of the second cylinder 11;The
In the first cylinder 10 of movable insertion of two cylinder 11, first cylinder 10 is fixedly connected with the second cylinder 11 by fixture 12.Cause
This, the depth of the first cylinder 10 is inserted by adjusting the second cylinder 11, can adjust height of the seed plummer 3 in camera bellows 1,
And then cause seed plummer 3 to go for the camera bellows 1 of different size.It is also possible to make the seed spectral detection system can
To detect to different types of seed, the use scene and scope of Seed inspection system is improve.Wherein, it is above-mentioned described
Fixture 12 can be arbitrary fixture 12 that the relative position of the first cylinder 10 and the second cylinder 11 can be fixed, example
Such as:Clip etc..
Further, can also include in above-mentioned camera bellows 1:Elastomeric element 13;Wherein, the elastomeric element 13 can be set in
The outside of the mounting post 9, and between the seed plummer 3 and the bottom of the camera bellows 1, for adjusting the second cylinder
11 and first cylinder 10 relative position when, Auxiliary support seed plummer 3.Wherein, elastomeric element mentioned here for example may be used
Think spring etc..The schematic diagram being illustrated that in Fig. 1 so that elastomeric element is as spring as an example.
Below with a specific example, to carry out to the function and effect of the seed spectral detection system of present invention offer
Detailed description, specifically:
By taking the fluorescence produced by the chlorophyll for obtaining seed to be detected as an example, examined using seed spectrum provided by the present invention
Illustrate as a example by examining system identification muskmelon seedses kind.Wherein, seed to be detected includes:36 extra white muskmelon seedses, 36
Individual one special gold muskmelon seedses, 36 capital No. 7 muskmelon seedses of honey, 36 capital No. 11 muskmelon seedses of honey and Elizabethan's muskmelon seedses.
Wherein, seed spectral detection system includes:Be arranged on seed plummer in camera bellows, LED light source unit, spectrogrph, optical filter,
Optical fiber, constant current source, radiator, and, processing equipment.Wherein, LED light source unit is for power 100W, a centre wavelength
660nm, the LED with a width of 40nm.Spectrogrph is the spectrogrph of QE65Pro models.Optical filter is for a centre wavelength
740nm, with a width of 10nm, transmitance is 75%, ends narrow band pass filter of the depth for OD4.Processing equipment is computer.
After above-mentioned seed to be detected is individually placed on seed object stage, start constant current source for the confession of LED light source unit
Electricity.LED light source unit after the preheating of 15 minutes, is sending stable red light irradiation seed to be detected, makes kind to be detected
Son produces reflected light and fluorescence.The reflected light and fluorescence of seed to be detected are transferred to spectrum after being filtered by narrow band pass filter
Instrument.The reflected light and fluorescence of spectrometer collection seed to be detected, obtains reflected light spectroscopic data and fluorescence data, during integration
Between be set to 10s.
Computer is received after the reflected light spectroscopic data and fluorescence data of spectrogrph transmission, first to reflected light
Spectroscopic data and fluorescence data are smoothed (for example:SG convolution smoothing algorithms), filtering (for example:Moving window is smooth to be calculated
Method), go trend (for example:Go trend Preprocessing Algorithm) and remove baseline drift (for example:Seek first derivative H algorithm) etc. process.
Then, computer reduces data dimension and extracts main constituent using principal component analytical method, the accumulation contribution of the main constituent of extraction
Rate is more than 85%.Using Kennard-Stone algorithms by seed to be detected be divided into training set and checking collection two groups, training set with test
The sample number ratio of card collection is 3:1, training set includes 135 parts of seeds to be detected, and checking collection includes 45 parts of seeds to be detected.Most
Afterwards, computer sets up muskmelon seedses variety ecotype model using linear discriminant analysiss method, to recognize the kind of seed to be detected.
Wherein, seed spectral detection system of the present invention adopts the accuracy rate of reflectance spectrum combined with fluorescent spectral matching factor muskmelon seedses kind,
Contrast with the accuracy rate that muskmelon seedses kind is recognized only with fluorescence spectrum is as described in Table 1:
Table 1
By the contrast of upper table 1, seed spectral detection system of the present invention is known using reflectance spectrum combined with fluorescent spectrum
The accuracy rate of other muskmelon seedses kind more preferably, that is, improves the recognition accuracy of muskmelon seedses kind.
The seed spectral detection system that the present invention is provided, by LED light source unit the fluorescence of seed generation to be detected is excited
And reflected light, so that spectrogrph can get the fluorescence data of the seed to be detected and reflected light spectroscopic data,
And then allow processing equipment that the testing result of seed to be detected is obtained based on the two spectroscopic datas.In this way,
Allow seed spectral detection system in the case where seed to be detected is not destroyed, the detection of seed to be detected can be got
As a result, the Non-Destructive Testing to seed to be detected is realized.Meanwhile, processing equipment obtains kind to be detected based on the two spectroscopic datas
The testing result of son is more accurate.
The schematic flow sheet of the seed spectral method of detection embodiment one that Fig. 2 is provided for the present invention.The embodiment of the present invention is related to
And be how Non-Destructive Testing to be carried out to seed to be detected.The executive agent of the present embodiment can compose detecting system for seed light.
As shown in Fig. 2 the method can include:
S101, monochromatic light is sent to seed to be detected, to excite seed to produce reflected light and fluorescence.
S102, collection reflected light and fluorescence, obtain reflected light spectroscopic data and fluorescence data.
S103, according to reflected light spectroscopic data and fluorescence data, obtain the testing result of seed to be detected.
The seed spectral method of detection that the present invention is provided, in can apply to above-mentioned seed spectral detection system, its realization
Principle is similar with technique effect, will not be described here.
The seed spectral method of detection that the present invention is provided, by sending monochromatic light to seed, to excite seed to be detected to produce
Raw fluorescence and reflected light, such that it is able to get the fluorescence data and reflected light spectroscopic data of the seed to be detected, enter
And the testing result that the two spectroscopic datas obtain seed to be detected can be based on.In this way, can treat not destroying
In the case of detection seed, the testing result of seed to be detected is got, realize the Non-Destructive Testing to seed to be detected.
One of ordinary skill in the art will appreciate that:Realizing all or part of step of said method embodiment can pass through
Completing, aforesaid program can be stored in a computer read/write memory medium the related hardware of programmed instruction, the program
Upon execution, the step of including said method embodiment is performed;And aforesaid storage medium includes:ROM, RAM, magnetic disc or light
Disk etc. is various can be with the medium of store program codes.
Finally it should be noted that:Various embodiments above only to illustrate technical scheme, rather than a limitation;To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that:Its according to
So the technical scheme described in foregoing embodiments can be modified, either which part or all technical characteristic are entered
Row equivalent;And these modifications or replacement, do not make the essence disengaging various embodiments of the present invention technology of appropriate technical solution
The scope of scheme.
Claims (8)
1. a kind of seed spectral detection system, it is characterised in that include:Camera bellows and processing equipment;
Include in the camera bellows:Seed plummer, LED light source cell, spectrogrph, power supply;
The power supply is electrically connected with the LED light source unit;
The LED light source unit to the seed to be detected on the seed plummer sends monochromatic light, to excite the seed to produce
Raw reflected light and fluorescence;
Reflected light described in the spectrometer collection and the fluorescence, obtain reflected light spectroscopic data and fluorescence data;
The spectrogrph is electrically connected with the processing equipment, and the spectrogrph is by the reflected light spectroscopic data and the fluorescence light
Modal data is transferred to the processing equipment.
2. system according to claim 1, it is characterised in that also include in the camera bellows:Optical filter and optical fiber;
The optical filter is connected by the optical fiber with the spectrogrph;
Reflected light and fluorescence of the spectrometer collection after optical filter filtration.
3. system according to claim 1, it is characterised in that the quantity of the LED light source unit is one or more.
4. system according to claim 3, it is characterised in that the quantity of the LED light source unit for it is multiple when, Duo Gesuo
State LED light source unit to be uniformly distributed as the center of circle with the central shaft of the seed plummer.
5. system according to claim 1, it is characterised in that the seed plummer is fixedly mounted on institute by mounting post
State the bottom of camera bellows;
The mounting post includes:First cylinder, the second cylinder, fixture, the bottom of first cylinder is fixed on the camera bellows
Bottom, and first cylinder is hollow cylinder, the external diameter of the internal diameter of first cylinder more than second cylinder;
In the movable insertion of second cylinder first cylinder, by the fixture by first cylinder and described second
Cylinder is fixedly connected.
6. system according to claim 5, it is characterised in that also include in the camera bellows:Elastomeric element;
The elastomeric element is set in mounting post outside, and positioned at the bottom of the seed plummer and the camera bellows it
Between.
7. system according to claim 1, it is characterised in that be additionally provided with the camera bellows and dissipated for the LED light source unit
The radiator of heat.
8. a kind of seed spectral method of detection, it is characterised in that include:
Monochromatic light is sent to seed to be detected, to excite the seed to produce reflected light and fluorescence;
The reflected light and the fluorescence are gathered, reflected light spectroscopic data and fluorescence data is obtained;
According to the reflected light spectroscopic data and the fluorescence data, the testing result of the seed to be detected is obtained.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611000759.5A CN106610377B (en) | 2016-11-14 | 2016-11-14 | Seed spectral method of detection and system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611000759.5A CN106610377B (en) | 2016-11-14 | 2016-11-14 | Seed spectral method of detection and system |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106610377A true CN106610377A (en) | 2017-05-03 |
CN106610377B CN106610377B (en) | 2019-11-15 |
Family
ID=58635913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611000759.5A Active CN106610377B (en) | 2016-11-14 | 2016-11-14 | Seed spectral method of detection and system |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106610377B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113176226A (en) * | 2021-03-19 | 2021-07-27 | 北京农业智能装备技术研究中心 | Portable seed quality spectrum detection system and method |
CN116137990A (en) * | 2023-04-19 | 2023-05-23 | 黑龙江省农业科学院绥化分院 | Detection device for germination capacity of rice seeds |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1484761A (en) * | 2000-10-30 | 2004-03-24 | ������ɽ���� | Method and device for anal yzing agricul products |
CN2662236Y (en) * | 2003-12-23 | 2004-12-08 | 广东省农业科学院作物研究所 | A peanut simple grain diffuse reflection spectrum measurement device |
CN101084707A (en) * | 2007-07-02 | 2007-12-12 | 华南师范大学 | Method for detecting vitality of seeds rapidly |
WO2009118111A1 (en) * | 2008-03-25 | 2009-10-01 | Cognis Ip Management Gmbh | Method and apparatus for determining seed germination |
CN103743705A (en) * | 2014-01-28 | 2014-04-23 | 盐城工学院 | Rapid detection method for sorghum halepense and similar species |
CN105486669A (en) * | 2016-01-12 | 2016-04-13 | 丽水学院 | Chlorophyll fluorescence diagnostic method for deficiency of indispensable plant nutritive elements |
-
2016
- 2016-11-14 CN CN201611000759.5A patent/CN106610377B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1484761A (en) * | 2000-10-30 | 2004-03-24 | ������ɽ���� | Method and device for anal yzing agricul products |
CN2662236Y (en) * | 2003-12-23 | 2004-12-08 | 广东省农业科学院作物研究所 | A peanut simple grain diffuse reflection spectrum measurement device |
CN101084707A (en) * | 2007-07-02 | 2007-12-12 | 华南师范大学 | Method for detecting vitality of seeds rapidly |
WO2009118111A1 (en) * | 2008-03-25 | 2009-10-01 | Cognis Ip Management Gmbh | Method and apparatus for determining seed germination |
CN103743705A (en) * | 2014-01-28 | 2014-04-23 | 盐城工学院 | Rapid detection method for sorghum halepense and similar species |
CN105486669A (en) * | 2016-01-12 | 2016-04-13 | 丽水学院 | Chlorophyll fluorescence diagnostic method for deficiency of indispensable plant nutritive elements |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113176226A (en) * | 2021-03-19 | 2021-07-27 | 北京农业智能装备技术研究中心 | Portable seed quality spectrum detection system and method |
CN113176226B (en) * | 2021-03-19 | 2023-05-12 | 北京农业智能装备技术研究中心 | Portable seed quality spectrum detection system and method |
CN116137990A (en) * | 2023-04-19 | 2023-05-23 | 黑龙江省农业科学院绥化分院 | Detection device for germination capacity of rice seeds |
Also Published As
Publication number | Publication date |
---|---|
CN106610377B (en) | 2019-11-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106520535B (en) | A kind of label-free cell detection device and method based on mating plate illumination | |
CN102565023B (en) | Device and method for fruit and vegetable pesticide residues based on laser Raman spectrometer | |
US8253936B2 (en) | Raman characterization of transplant tissue | |
US7477378B2 (en) | Method for correlating spectroscopic measurements with digital images of contrast enhanced tissue | |
CN1910442A (en) | Method and apparatus for testing fibres | |
Guze et al. | Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity | |
CN104764727A (en) | Fluorescence imaging analysis system and fluorescence imaging analysis method thereof | |
CN100547383C (en) | A kind of laser double-mode micro-volume sample analyzing method and equipment therefor | |
JP2021105616A (en) | Optical detection systems, and methods of using the same | |
CN106092994A (en) | A kind of micro-array chip fluorescence detection method of great power LED | |
CN106610377A (en) | Seed spectral detection method and system | |
CN101196464B (en) | Laser double-mode micro-volume sample analyzing method and its device | |
CN105954257A (en) | Intelligent human body calculus component analyzer and application method thereof | |
CN104568824A (en) | Method and device for detecting freshness grade of shrimps based on visible/near-infrared spectroscopy | |
CN204556502U (en) | A kind of fluorescence imaging analysis system | |
CN102095685A (en) | Photoacoustic component resolution imaging method and device based on spectral encoding | |
CN1474175B (en) | Super fine optical spectrum imaging instrument or system | |
Appalaneni et al. | Single fiber identification with nondestructive excitation–emission spectral cluster analysis | |
CN101911877B (en) | Seed vitality authentication device and method based on laser light diffuse reflection image technology | |
CN105335425A (en) | Fibrosis value assessing method and apparatus and fibrosis dynamic assessing method and system | |
Legesse et al. | Investigation of microalgal carotenoid content using coherent anti‐Stokes Raman scattering (CARS) microscopy and spontaneous Raman spectroscopy | |
CN104181133A (en) | Multi-wavelength capillary electrophoresis fluorescence excitation device | |
CN109612964A (en) | Eliminate the spectral method of detection of the scattering of cultivation matrix particle surface and moisture influence | |
CN110333211B (en) | Device and method for detecting color and maturity of cotton fiber | |
CN106324242A (en) | Quantum dot test strip quality detection system and method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |