CN106609241A - Cell negative pressure culture apparatus - Google Patents
Cell negative pressure culture apparatus Download PDFInfo
- Publication number
- CN106609241A CN106609241A CN201510681508.7A CN201510681508A CN106609241A CN 106609241 A CN106609241 A CN 106609241A CN 201510681508 A CN201510681508 A CN 201510681508A CN 106609241 A CN106609241 A CN 106609241A
- Authority
- CN
- China
- Prior art keywords
- culture
- culture fluid
- housing
- negative
- negative pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The present invention discloses a culture apparatus capable of performing cell culture in a direct negative pressure environment. The culture apparatus comprises: a shell for holding a culture liquid; a bio-semipermeable membrane for cell attachment, wherein the middle portion is high while the periphery is low, and air and water molecules can permeate through the bio-semipermeable membrane; a culture liquid injection device, wherein the spraying speed of the culture liquid sprayed to the bio-semipermeable membrane is controlled through a liquid flow rate valve; a gas inlet for conveying oxygen and carbon dioxide into the culture apparatus; a gas outlet connected to a negative pressure source so as to form a negative pressure in the culture apparatus; and a liquid draining valve for discharging the excess culture liquid. According to the present invention, the culture apparatus has characteristics of simple structure, low manufacturing cost and easy operation, wherein the cells are inoculated onto the biofilm, and the negative pressure source is connected, such that the cells are cultured in the negative pressure environment.
Description
Technical field
The present invention relates to a kind of cell culture experiments instrument and equipment, the cell culture incubator under particularly a kind of subnormal ambient.
Background technology
The reparation of skin tissue defects is always numerous medical personnels and patient problem deeply concerned.Recent decades, it is negative-pressure sealed
Drainage technology has obtained widely applying in terms for the treatment of skin tissue defects, has numerous studies to find that there is promotion defect to repair for it
Multiple effect.In order to study the mechanism that negative-pressure sealed drainage technology promotes wound repair, extensive work person devises various devices pair
Cell applies tensile force imitating cell living environment under negative pressure, and its tensile force effect to cell is generally by making cell
The base material of adhesion deforms upon to realize.(Leung D Y, Glagov S, Matthews M B.A new in vitro system
for studying cell response to mechanical stimulation.Different effects of cyclic
stretching and agitation on smooth muscle cell biosynthesis[J].Exp
Cell Res, 1977,109 (2)：285-298) (Carosi J A, Eskin S G, Mcintire L V.Cyclical
strain effects on the production of vasoactive material in endothelial cells[J].J
Cell Physiol, 1992,151 (1)：29-36) (Neidlinger-Wilke C, Grood E S, Wang J H, et al.Cell
alignment is induced by cyclic changes in cell length：studies of cells grown in
Cyclically stretched subtrates [J] .J Orthop Res, 2001,19 (2)：286-293) (Kaspar D,
Seidl W, Neidl inger-Wilke C, et al.Dynamic cell stretching increases human osteoblast
proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline
Phosphatase activity [J] .J Biomech, 2000,33 (1)：45-51).The stretching dress of Flexercell companies
Put, be also to control cell stress size by adjusting the VERTICAL DEFORMATION of flexible cell culture plate basement membrane.
In existing invention at present and device, being in cell in culture fluid more, and the cell in human body wound surface is directly in negative pressure ring
Under border, the cell in research in the past in culture fluid will be affected by culture fluid, cell in current cell culture system
Biological behaviour can not well react change of the wound surface inner cell under suction function.
The content of the invention
The present invention provides a kind of culture apparatuses that cell culture can be carried out under direct subnormal ambient, and cell can be made to be in a simulation
The environment of negative pressure treatment.
A kind of negative-pressure cell culture apparatus, including for holding the housing of culture fluid；Also include in housing for cell attachment
Biological semi-permeable film, culture fluid injection device, air inlet and gas outlet, liquid drain valve.
The described housing for holding culture fluid is transparent material, is made up of two parts, and two parts are connected by thread seal.
Described biological semi-permeable film is located at the bottom of housing the latter half, is the low mound shape structure of the high surrounding in middle part, and semipermeable membrane can lead to
Cross air and hydrone.
Described culture fluid injection device is located at the roof of casing upper half point, and culture fluid inlet is gondola water faucet spline structure, can be in weight
Power effect is lower to be continued to spray culture fluid into housing, has liquid flow valve to control the flow velocity of culture fluid, culture on culture fluid inlet
Liquid injection device bottom has culture fluid to add mouth, when culture fluid is not enough in culture fluid injection device, by the addition mouth addition training
Nutrient solution.
Described air inlet is located at the side wall of housing the latter half, can be held into housing with external oxygen and carbon dioxide conveyance conduit
It is continuous to send oxygen, for cell growth needs.
Described gas outlet is located at the side wall of housing the latter half, can be made in housing in corresponding negative pressure ring with external connecting negative pressure source
In border.
Described liquid drain valve is located at housing sidewall lowest order, is easy to culture fluid excessive in housing to discharge.
The cell culture system of the present invention, by the way that cell is inoculated on the shape biological semi-permeable film of mound, the culture being sprayed on semipermeable membrane
Liquid will flow to the low-lying place of surrounding along the abrupt slope of mound shape structure, and enter space below by semipermeable membrane, external negative in gas outlet
During potential source, the cell on semipermeable membrane will bear directly against under subnormal ambient, realize the mould to wound surface cell environment under negative pressure treatment
Intend.
Description of the drawings
Fig. 1 is the negative-pressure cell culture apparatus side structure schematic diagram of the present invention.
In figure：1 is the housing the latter half for holding culture fluid, and 2 is that the casing upper half for holding culture fluid divides 3 for biological semipermeable membrane,
4 is culture fluid injection device, and 5 is air inlet, and 6 is gas outlet, and 7 is liquid drain valve, and 8 is liquid flow valve, and 9 are culture
Liquid adds mouth.
Specific embodiment
Housing the latter half (1) is separated with casing upper half point (2), will treat that cultured cells is inoculated in biological semi-permeable film (3)
On, screw thread screws housing the latter half (1) and casing upper half point (2), by the air inlet of housing the latter half (1) side wall
(4) it is connected with oxygen and carbon dioxide conduit, the gas outlet (5) of housing the latter half (1) side wall is connected with negative pressure source,
Add mouth (9) by culture fluid culture fluid to be added in culture fluid injection device (4), open liquid flow valve (8) control training
Nutrient solution spray rate, makes culture fluid be sprayed on biological semi-permeable film (3), and opening external connecting negative pressure source carries out cell training under subnormal ambient
Support, when culture fluid is more in housing, open liquid drain valve (7), give off unnecessary culture fluid, it is to avoid cell is immersed in
In culture fluid.Under apparatus of the present invention, impact of the culture fluid to cell can be excluded, so as to study cell under subnormal ambient
Biological behaviour.
The invention is not restricted to the scope of embodiment, as long as can realize that cell culture is all at this under the subnormal ambient affected without culture fluid
Within bright protection domain.
Claims (8)
1. a kind of negative-pressure cell culture apparatus, including for holding the housing of culture fluid；For the biological semi-permeable film of cell attachment, middle part
High surrounding is low, can be by air and hydrone；Culture fluid injection device, controls to be trained to biomembrane sprinkling by liquid flow valve
The speed of nutrient solution；Air inlet, for conveying oxygen and carbon dioxide into culture apparatuses；Gas outlet, for connecting negative pressure source
Make to form negative pressure in culture apparatuses；Liquid drain valve, for the discharge of unnecessary culture fluid；It is characterized in that seeding cells into
Biomembrane, after connection negative pressure source, cell can carry out cell culture in the environment of negative pressure.
2. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：The described housing for holding culture fluid is transparent material,
It is made up of upper and lower two parts, two parts are connected by thread seal.
3. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：Described biological semi-permeable film is located at housing the latter half
Bottom, be the low mound shape structure of the high surrounding in middle part, semipermeable membrane can be by air and hydrone.
4. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：Described culture fluid injection device is located at housing upper half
Partial roof, culture fluid inlet is gondola water faucet spline structure, can continue under gravity to spray culture fluid into housing.
5. culture fluid injection device according to claim 4, it is characterised in that：There is liquid flow valve control culture on culture fluid inlet
The flow velocity of liquid, culture fluid injection device bottom has culture fluid to add mouth, when culture fluid is not enough in culture fluid injection device, leads to
Cross the addition mouth addition culture fluid.
6. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：Described air inlet is located at the side of housing the latter half
Wall, can persistently send oxygen into housing with external oxygen and carbon dioxide conveyance conduit, for cell growth needs.
7. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：Described gas outlet is located at the side of housing the latter half
Wall, can make to be in corresponding subnormal ambient in housing with external connecting negative pressure source.
8. negative-pressure cell culture apparatus according to claim 1, it is characterised in that：It is minimum that described liquid drain valve is located at housing sidewall
Position, is easy to culture fluid excessive in housing to discharge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510681508.7A CN106609241A (en) | 2015-10-21 | 2015-10-21 | Cell negative pressure culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510681508.7A CN106609241A (en) | 2015-10-21 | 2015-10-21 | Cell negative pressure culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106609241A true CN106609241A (en) | 2017-05-03 |
Family
ID=58610281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510681508.7A Pending CN106609241A (en) | 2015-10-21 | 2015-10-21 | Cell negative pressure culture apparatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN106609241A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849771A (en) * | 2020-07-31 | 2020-10-30 | 中国人民解放军空军军医大学 | Culture device for carrying out negative pressure and mechanical stimulation on cell-containing culture |
| GB2620504A (en) * | 2020-11-24 | 2024-01-10 | Micropropagation Services E M Ltd | Apparatus and methods for culturing Sphagnum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665768A (en) * | 2008-09-05 | 2010-03-10 | 中国人民解放军第三军医大学第二附属医院 | Bioreactor for in vitro culture of neurotrophic factors |
| CN101665766A (en) * | 2009-09-25 | 2010-03-10 | 中国人民解放军第三军医大学第一附属医院 | Negative-pressure cell culture apparatus and method thereof |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
-
2015
- 2015-10-21 CN CN201510681508.7A patent/CN106609241A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665768A (en) * | 2008-09-05 | 2010-03-10 | 中国人民解放军第三军医大学第二附属医院 | Bioreactor for in vitro culture of neurotrophic factors |
| CN101665766A (en) * | 2009-09-25 | 2010-03-10 | 中国人民解放军第三军医大学第一附属医院 | Negative-pressure cell culture apparatus and method thereof |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849771A (en) * | 2020-07-31 | 2020-10-30 | 中国人民解放军空军军医大学 | Culture device for carrying out negative pressure and mechanical stimulation on cell-containing culture |
| CN111849771B (en) * | 2020-07-31 | 2023-03-28 | 中国人民解放军空军军医大学 | Culture device for carrying out negative pressure and mechanical stimulation on cell-containing culture |
| GB2620504A (en) * | 2020-11-24 | 2024-01-10 | Micropropagation Services E M Ltd | Apparatus and methods for culturing Sphagnum |
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Application publication date: 20170503 |
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