CN106596692A - 一种弯曲毛细管电泳检测抗体多肽相互作用的方法 - Google Patents
一种弯曲毛细管电泳检测抗体多肽相互作用的方法 Download PDFInfo
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- CN106596692A CN106596692A CN201710026653.0A CN201710026653A CN106596692A CN 106596692 A CN106596692 A CN 106596692A CN 201710026653 A CN201710026653 A CN 201710026653A CN 106596692 A CN106596692 A CN 106596692A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
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CN201710026653.0A CN106596692B (zh) | 2017-01-14 | 2017-01-14 | 一种弯曲毛细管电泳检测抗体多肽相互作用的方法 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110044988A (zh) * | 2019-04-26 | 2019-07-23 | 常州大学 | 一种弯曲毛细管电泳检测多酶的方法 |
CN110741250A (zh) * | 2017-06-13 | 2020-01-31 | 基因微器件有限公司 | 改变电场的电泳方法 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0597152A1 (en) * | 1992-11-13 | 1994-05-18 | Jean-Pierre Chervet | Optimizing capillary flow cells |
WO1999024828A1 (en) * | 1997-11-12 | 1999-05-20 | The Perkin-Elmer Corporation | Serpentine electrophoresis channel with self-correcting bends |
CN1769884A (zh) * | 2005-09-28 | 2006-05-10 | 哈尔滨工业大学 | 一种压丝法制作集成有电化学检测电极的毛细管电泳芯片 |
CN101493413A (zh) * | 2009-01-15 | 2009-07-29 | 浙江大学 | 转动扫描毛细管阵列分析仪 |
CN102998357A (zh) * | 2012-10-15 | 2013-03-27 | 常州大学 | 一种毛细管电泳检测抗体与多肽相互作用的方法 |
CN103108701A (zh) * | 2010-09-26 | 2013-05-15 | 大玉企业有限公司 | 分析物的分离 |
CN103111338A (zh) * | 2013-02-02 | 2013-05-22 | 中国计量学院 | 一种消除毛细管电泳槽道中弯道扩散效应的方法 |
CN104646079A (zh) * | 2015-02-17 | 2015-05-27 | 方雪萍 | 一种用于毛细管电泳的离心式cd微流控芯片及毛细管凝胶电泳装置 |
CN104745446A (zh) * | 2013-12-31 | 2015-07-01 | 窦晓鸣 | 一种pcr和毛细管电泳集成的微流控芯片及采用该芯片的病原菌快速检测装置 |
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2017
- 2017-01-14 CN CN201710026653.0A patent/CN106596692B/zh active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0597152A1 (en) * | 1992-11-13 | 1994-05-18 | Jean-Pierre Chervet | Optimizing capillary flow cells |
US5423513A (en) * | 1992-11-13 | 1995-06-13 | Lc Packings International | Method of and a capillary flow cell for analysing fluid samples |
WO1999024828A1 (en) * | 1997-11-12 | 1999-05-20 | The Perkin-Elmer Corporation | Serpentine electrophoresis channel with self-correcting bends |
CN1769884A (zh) * | 2005-09-28 | 2006-05-10 | 哈尔滨工业大学 | 一种压丝法制作集成有电化学检测电极的毛细管电泳芯片 |
CN101493413A (zh) * | 2009-01-15 | 2009-07-29 | 浙江大学 | 转动扫描毛细管阵列分析仪 |
CN103108701A (zh) * | 2010-09-26 | 2013-05-15 | 大玉企业有限公司 | 分析物的分离 |
CN102998357A (zh) * | 2012-10-15 | 2013-03-27 | 常州大学 | 一种毛细管电泳检测抗体与多肽相互作用的方法 |
CN103111338A (zh) * | 2013-02-02 | 2013-05-22 | 中国计量学院 | 一种消除毛细管电泳槽道中弯道扩散效应的方法 |
CN104745446A (zh) * | 2013-12-31 | 2015-07-01 | 窦晓鸣 | 一种pcr和毛细管电泳集成的微流控芯片及采用该芯片的病原菌快速检测装置 |
CN104646079A (zh) * | 2015-02-17 | 2015-05-27 | 方雪萍 | 一种用于毛细管电泳的离心式cd微流控芯片及毛细管凝胶电泳装置 |
Non-Patent Citations (1)
Title |
---|
TASANEE SRICHAIYO AND STELLAN HJERTEN: "Simple multi-point detection method for high-performance capillary electrophoresis", 《JOURNAL OF CHROMATOGRAPHY》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110741250A (zh) * | 2017-06-13 | 2020-01-31 | 基因微器件有限公司 | 改变电场的电泳方法 |
CN110741250B (zh) * | 2017-06-13 | 2024-03-26 | 基因微器件有限公司 | 改变电场的电泳方法 |
CN110044988A (zh) * | 2019-04-26 | 2019-07-23 | 常州大学 | 一种弯曲毛细管电泳检测多酶的方法 |
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Effective date of registration: 20231227 Address after: Room 203A, 2nd Floor, No. 229 Gongchang Road, Zhenmei Community, Xinhu Street, Guangming District, Shenzhen City, Guangdong Province, 518000 Patentee after: Shenzhen Jiebo Biotechnology Co.,Ltd. Address before: 230000 floor 1, building 2, phase I, e-commerce Park, Jinggang Road, Shushan Economic Development Zone, Hefei City, Anhui Province Patentee before: Dragon totem Technology (Hefei) Co.,Ltd. Effective date of registration: 20231227 Address after: 230000 floor 1, building 2, phase I, e-commerce Park, Jinggang Road, Shushan Economic Development Zone, Hefei City, Anhui Province Patentee after: Dragon totem Technology (Hefei) Co.,Ltd. Address before: Gehu Lake Road Wujin District 213164 Jiangsu city of Changzhou province No. 1 Patentee before: CHANGZHOU University |
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