CN106596665B - 一种氧化铜掺氮石墨烯气凝胶的应用 - Google Patents
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Abstract
本发明涉及一种水热法合成氧化铜掺氮石墨烯气凝胶的应用。包括以下步骤：氧化铜掺氮石墨烯气凝胶修饰电极的制备，氧化铜掺氮石墨烯气凝胶用于葡萄糖的电化学检测，氧化铜掺氮石墨烯气凝胶对于葡萄糖电化学检测中干扰物的抗干扰测试。本发明的有益效果是氧化铜掺氮石墨烯气凝胶对于葡萄糖的电化学检测具有较宽的检测范围、较低的灵敏度和优异的抗干扰能力。
Description
技术领域
本发明涉及一种氧化铜掺氮石墨烯气凝胶的应用，属于电分析化学的研究领域。
技术背景
葡萄糖是人体重要的能量来源，在糖尿病诊断过程中，精确高效的检测血糖中葡萄糖浓度对临床诊断具有重要的意义。在目前所具有的检测方法中，电化学检测是最具有吸引力的一种方法，它具有成本低、易操作、效率高等优点。
自1962年第一个利用葡萄糖氧化酶制备的葡萄糖免疫传感器的报道以来，研究者已经研究了大量关于葡萄糖氧化酶的免疫传感器，它们具有良好的生物活性和较高的灵敏度，但是葡萄糖氧化酶的免疫传感器成本较高，且稳定性也较差。基于酶传感器的这些缺点我们开始研究无酶葡萄糖免疫传感器。
目前为止关于氧化铜纳米粒子与掺氮石墨烯气凝胶复合材料用于葡萄糖检测的研究未有报道，所以通过氧化铜掺氮石墨烯气凝胶修饰电极制备无酶免疫传感器应用于葡萄糖的检测。因复合材料兼有氧化铜和掺氮石墨烯的优点具有更大的比表面积、更高的电子转移率、更好的电化学催化活性，使其对于葡萄糖的检测具有较低的检测限、较高的灵敏度和较宽的线性范围。
发明内容
本发明的目的是在于提供一种氧化铜掺氮石墨烯气凝胶在葡萄糖检测中的应用。
本发明所述一种氧化铜掺氮石墨烯气凝胶的应用，包括以下步骤：
1、一种氧化铜掺氮石墨烯气凝胶修饰电极的制备，其特征在于：步骤如下：
氧化铜掺氮石墨烯气凝胶修饰电极的制备：将氧化铜掺氮石墨烯气凝胶超声分散于超纯水中，然后将分散液滴涂到电极上，室温下干燥，得到氧化铜掺氮石墨烯气凝胶修饰的电极。
2、氧化铜掺氮石墨烯气凝胶的应用，其特征在于：步骤如下：
a、氧化铜掺氮石墨烯气凝胶修饰电极对于葡萄糖检测性能的测试：本发明使用氧化铜掺氮石墨烯气凝胶修饰电极通过电化学法对葡萄糖进行电化学检测，本实验采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片为对电极，饱和甘汞电极为参比电极，电解液为氢氧化钠溶液，在以上条件下进行葡萄糖的电流-时间曲线测试，并通过电流-时间曲线计算出氧化铜掺氮石墨烯气凝胶修饰电极用于葡萄糖检测时的检测限，灵敏度和线性范围；
b、氧化铜掺氮石墨烯气凝胶修饰电极对于葡萄糖检测抗干扰性能的测试：本发明使用氧化铜掺氮石墨烯气凝胶修饰电极通过电化学法对葡萄糖检测时抗干扰性能的测试，本实验采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片为对电极，饱和甘汞电极为参比电极，氢氧化钠溶液为电解液，在以上条件下进行葡萄糖的电流-时间曲线测试，测试过程中，每隔60s向电解液中分别加入不同的干扰物质考察对葡萄糖电流响应值的影响。
进一步，所述步骤1中分散液的体积为5～10μL，浓度为1～4mg/mL。
进一步，所述步骤2(a)和(b)中电化学测试中氢氧化钠电解液浓度为0.1～0.3mol/L。
本发明的有益效果是：氧化铜掺氮石墨烯气凝胶对于葡萄糖的检测具有较宽的线性范围、较低的灵敏度和优异的抗干扰能力。
附图说明
下面结合附图对本发明进一步说明。
图1为实施例一中氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖检测的电流-时间曲线；
图2为实施例一中根据氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖检测的电流-时间曲线而绘制的线性曲线；
图3为实施例二中氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖检测时添加有机物干扰物时的电流-时间曲线；
图4为实施例二中氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖检测时添加无机离子干扰物时的电流-时间曲线。
具体实施方式
现在结合具体实施例对本发明做进一步说明，以下实施例旨在说明本发明而不是对本发明的进一步限定。
实施例一：
本发明使用氧化铜掺氮石墨烯气凝胶通过电化学法对葡萄糖的性能进行检测，本实验采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片电极为对电极，饱和甘汞电极为参比电极，电解液为氢氧化钠溶液，在以上条件下进行电流-时间曲线测试，并通过电流-时间曲线图计算氧化铜掺氮石墨烯气凝胶对葡萄糖检测的检测限、灵敏度和检测范围。从附图1和图2所知，该氧化铜掺氮石墨烯气凝胶对葡萄糖检测限是2.7μM，灵敏度为228.9μA mM-1cm-2，线性范围达到10μM～760μM。
实施例二：
本发明使用氧化铜掺氮石墨烯气凝胶通过电化学法对葡萄糖检测时抗干扰性能的研究，本实验采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片电极为对电极，饱和甘汞电极为参比电极，氢氧化钠溶液为电解液，在以上条件下进行电流-时间曲线测试，加入不同干扰物时氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖的电流响应值。从附图3和图4所知，在加入干扰物质之后再检测葡萄糖，该氧化铜掺氮石墨烯气凝胶修饰电极对葡萄糖仍然具有较高的电流响应，干扰物质造成电流的变化小于3.6％。
Claims (4)
1.一种氧化铜掺氮石墨烯气凝胶修饰电极的制备，其特征在于：步骤如下：
氧化铜掺氮石墨烯气凝胶修饰电极的制备：将氧化铜掺氮石墨烯气凝胶超声分散于超纯水中，然后将分散液滴涂到电极上，室温下干燥，得到该氧化铜掺氮石墨烯气凝胶修饰的电极。
2.根据权利要求1所述一种氧化铜掺氮石墨烯气凝胶修饰电极的制备，所述步骤中分散液的体积为5～10μL，浓度为1～4mg/mL。
3.氧化铜掺氮石墨烯气凝胶修饰电极的应用，其特征在于：步骤如下：
a、氧化铜掺氮石墨烯气凝胶修饰电极对于葡萄糖的电化学检测：使用氧化铜掺氮石墨烯气凝胶修饰电极通过电化学法对葡萄糖进行电化学检测，电化学检测采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片为对电极，饱和甘汞电极为参比电极，电解液为氢氧化钠溶液，在以上条件下进行葡萄糖的电流-时间曲线测试，并通过电流-时间曲线计算出氧化铜掺氮石墨烯气凝胶修饰电极用于葡萄糖检测时的检测限，灵敏度和线性范围；
b、氧化铜掺氮石墨烯气凝胶修饰电极对于葡萄糖检测抗干扰性能的测试：使用氧化铜掺氮石墨烯气凝胶修饰电极通过电化学法对葡萄糖检测时抗干扰性能的测试，抗干扰性能测试采用三电极体系，氧化铜掺氮石墨烯气凝胶修饰电极为工作电极，铂片为对电极，饱和甘汞电极为参比电极，氢氧化钠溶液为电解液，在以上条件下进行葡萄糖的电流-时间曲线测试，测试过程中，每隔60s向电解液中分别加入不同的干扰物质考察对葡萄糖电流响应值的影响。
4.根据权利要求3所述氧化铜掺氮石墨烯气凝胶修饰电极的应用，其特征是：所述步骤a和b中电化学测试中氢氧化钠电解液浓度为0.1～0.3mol/L。
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