CN106591212A - Method for producing conidia by Pochonia chlamydosporia liquid fermentation - Google Patents
Method for producing conidia by Pochonia chlamydosporia liquid fermentation Download PDFInfo
- Publication number
- CN106591212A CN106591212A CN201611262188.2A CN201611262188A CN106591212A CN 106591212 A CN106591212 A CN 106591212A CN 201611262188 A CN201611262188 A CN 201611262188A CN 106591212 A CN106591212 A CN 106591212A
- Authority
- CN
- China
- Prior art keywords
- spore
- liquid fermentation
- liquid
- thick wall
- keniya
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
Landscapes
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for producing conidia by Pochonia chlamydosporia liquid fermentation. The method comprises activation of a Pochonia chlamydosporia strain, strain spore suspension liquid preparation and liquid fermentation production of conidia. A liquid fermentation culture medium comprises corn flour, sucrose, soybean meal and an inorganic salt. The method can produce 25.45*10<8>cfu/mL of conidia, has a high fermentation unit, utilizes cheap and easily available raw materials, realizes fermentation enlarge production based on the culture medium formula and provides the basis for use of a dried mixture of a spore fermented liquid and a matrix in the biological prevention and control of crop nematodosis.
Description
Technical field
A kind of conidiiferous method the present invention relates to thick wall spore Pu Keniya bacteria liquids ferment, belongs to fermentable
Field.
Background technology
Thick wall spore Pu Keniya bacterium（Pochonia chlamydosporia）Category Deuteromycotina
（Deuteromycotina）, four spore guiding principles（Hyphomycetes）, hyphomycetales（Moniliales）, Si Bao sections（Monilaceae）、
Pu Keni subgenus（Pochonia）, earliest by Goddard（1913）It is isolated from soil with dilution-plate method.
Nemas is the soil-borne disease that harm is larger and has a very wide distribution of developping production to agriculture and forestry.Traditional preventing and treating side
Method can not only destroy ecological environment using chemicals such as nematicides, or even its chemical residue causes food security into branch
Problem, and it is also dangerous to people and animals using process, therefore, such as chemical nematicides " Furadan " are disabled.Thick wall spore is general
Can Buddhist nun Asia bacterium be a kind of facultative parasite, can parasitize in root-knot nematode, SCN ovum and female adult body, also can be in soil
Battalion's saprogenesis, is a kind of larger fungi microbe of Biocontrol Potential.Based on the defect in terms of traditional root knot nematode control, with
And the advantage of biological control method, the method fermented using thick wall spore Pu Keniya bacteria liquids produces spore becomes Research Points.
The exploitation of biocontrol agent mainly includes strain breeding thereof, zymotechnique and medicament development etc., and zymotechnique is
Key link in biocontrol microorganisms development process, is also the basis of microbial inoculum large-scale production.It is presently used for thick wall spore Pu Keniya bacterium
Fermentation process mainly has liquid fermentation, solid-liquid double-phase fermentation.Liquid fermentation is primarily present the relatively low problem of conidium yield, and
For solid-liquid double-phase ferments compared to single fermentation, fermentation time is longer, and operation is relative complex, increases sweat microbiological contamination general
Rate.Liu Chunxiu etc.（Liu Chunxiu, Wang Laifa, Piao Chungen, etc. the research [J] of Verticillium chlamydosporium shaking flask liquid fermentation and culture.
Forestry scientific research, 2006,19 (2): 141-144.）Liquid Culture based formulas are carried out to the bacterium（Glucose 20g/L, corn
Powder 20g/L, analysis for soybean powder 20g/L, KH2PO40.5g/L、MgSO40.5g/L）After optimization, gained spore output be 8.25 ×
108cfu/mL；Wang Xizhuo etc.（Wang Xizhuo, Ma Jianwei, Wang Laifa, etc. thick wall spore Pu Keniya liquid fermentation dynamics
[J]. forest-science, 2014,50 (11): 75-81.）On the basis of the optimization of C/C composites such as Liu Chunxiu to formula in some groups
Point content is optimized adjustment（Glucose 15g/L, corn flour 15g/L, analysis for soybean powder 20g/L, KH2PO40.25g/L、
MgSO40.25g/L）, and the dynamic (dynamical) research of liquid fermentation is carried out using this formula, it is final obtain spore output be 10.53 ×
108cfu/mL。
The method provided using the present invention is fermented, and the conidial fermentation unit of gained is carried compared with the research of Wang Xizhuo etc.
High by 141.69%, operation is simple, and culture medium raw material is cheap and easy to get, can be thick wall spore Pu Keniya bacterium biological prevention and control agents
Production lays the foundation.
The content of the invention
It is an object of the invention to provide one kind can make thick wall spore Pu Keniya bacterium produce a large amount of conidial liquid depths
Layer fermentation process.
In order to realize the object of the invention, the conidiiferous side of thick wall spore Pu Keniya bacteria liquids fermentation that the present invention is provided
Method, including the step of thick wall spore Pu Keniya bacterium actication of culture, the preparation of bacterial classification spore suspension and liquid fermentation product spore；
Wherein, the liquid fermentation medium formula used in the liquid fermentation product spore step is：Corn flour 15-35 g, sugarcane
Sugared 5-20 g, bean cake powder 5-15 g, sodium chloride 1-10 g, the g of magnesium sulfate 0.1-1.0, the g of potassium chloride 0.1-1.0, ferrous sulfate
0.01 g, adds water to 1000mL.Liquid fermentation medium pH is adjusted to pH6.0 ± 0.2, with the formation of induced meristem spore.
Preferably, liquid fermentation medium formula is in the liquid fermentation product spore step：Corn flour 20-30 g, sucrose
8-15 g, bean cake powder 8-12 g, sodium chloride 3-8 g, magnesium sulfate 0.3-0.8 g, potassium chloride 0.3-0.8 g, ferrous sulfate 0.01
G, adds water to 1000mL.Liquid fermentation medium is adjusted to pH6.0 ± 0.2, with the formation of induced meristem spore.
It is further preferable that the liquid fermentation produces liquid fermentation medium formula in spore step being：The g of corn flour 25, sucrose
10 g, the g of bean cake powder 10, the g of sodium chloride 5, the g of magnesium sulfate 0.5, the g of potassium chloride 0.5, the g of ferrous sulfate 0.01, add water to
1000mL.Liquid fermentation medium is adjusted to pH6.0 ± 0.2, with the formation of induced meristem spore.
Thick wall spore Pu Keniya bacterium actication of culture steps are in preceding method：Thick wall spore Pu Keniya bacterium bacterial classifications are seeded in
Activation culture 5d on PDA inclined-planes, cultivation temperature is 28 DEG C.
The preparation method of bacterial classification spore bacteria suspension is in preceding method：
A. the thick wall spore Pu Keniya bacterium strain transfer Jing after slant activation is cultivated into eggplant-shape bottle, 28 DEG C of cultivation temperature, is trained
Foster time 5-7d；
B. aseptically in the eggplant-shape bottle add 15mL mass concentrations be 1.0% Tween-80, then lawn is scraped
Under be seeded to seed culture medium, 28 DEG C, 160r/min constant-temperature table culture 24h obtain final product bacterial classification spore suspension；
Wherein, the formula of liquid seed culture medium is in step b：The g of sucrose 30, the g of dusty yeast 0.5, the g of sodium nitrate 3.0, sulphur
The g of sour magnesium 0.5, the g of potassium chloride 0.5, the g of potassium dihydrogen phosphate 1.0, the g of ferrous sulfate 0.02, add water to 1000mL.
It is the step of liquid fermentation product spore in preceding method：The bacterial classification spore bacteria suspension is inoculated with by 10% inoculum concentration
Into the liquid fermentation medium, in constant-temperature table shaken cultivation, constant-temperature table shaken cultivation condition is：Shaking speed
160r/min, 28 DEG C of cultivation temperature, incubation time are 144-168h.
The thick wall spore Pu Keniya bacteria strains for being used for liquid fermentation product spore in the present invention are purchased from Chinese agriculture microorganism fungus kind
Preservation administrative center, deposit number is ACCC 30601.
The invention provides a kind of conidiiferous method of thick wall spore Pu Keniya bacteria liquid shake flask fermentations, thick wall spore is general
Can Buddhist nun Asia bacterium Jing actication of culture, the preparation of bacterial classification suspension, then fermentation is carried out with the method for liquid fermentation produce spore.The liquid is sent out
The fluid nutrient medium that ferment is used is made up of corn flour, sucrose, bean cake powder and inorganic salts, wherein corn flour, sucrose as carbon source and
Energy substance adds, and bean cake powder provides a great number of elements and micro unit as nitrogen source, inorganic salts for the bacteria growing sweat
The appropriate addition of element, wherein sodium chloride has an effect for adjusting osmotic pressure of fermentation liquor, and can inducing spore to a certain extent
Formed.The liquid fermentation culturing method provided using the present invention, can produce substantial amounts of conidium, and spore amount is reached as high as
25.45×108Cfu/mL, fermentation unit is high, and raw material are cheap and easy to get, low cost, can be further with the culture medium prescription
Fermentation scale-up production is carried out, and then is that the spore zymotic fluid that will be obtained is used for crops nematodiasis Jing after with matrix combination drying
Biological control provides basis.
Specific embodiment
Following examples are not limited to the scope of the present invention to being used to illustrate the present invention.If not specializing, implement
The conventional meanses that technological means used is well known to those skilled in the art in example, it is raw materials used to be commercial goods.
The liquid fermentation of embodiment 1 produces thick wall spore Pu Keniya bacterium conidiums
Comprise the steps：
The activation of 1 slant strains
Will be purchased from Chinese agriculture Culture Collection, deposit number is the thick wall spore Pu Keniya bacterium bacterium of ACCC 30601
Activation culture 5d on PDA inclined-planes is planted, cultivation temperature is 28 DEG C.
The preparation of 2 bacterial classification suspension
（1）By the bacterial classification Jing after activating on PDA inclined-planes, aseptically it is forwarded in PDA eggplant-shape bottles and cultivates, cultivation temperature
For 28 DEG C, incubation time 5-7d；
（2）Thick wall spore Pu Keniya bacteria liquid seed culture mediums are prepared, liquid seed culture medium formula is：The g of sucrose 30, yeast
The g of powder 0.5, the g of sodium nitrate 3.0, the g of magnesium sulfate 0.5, the g of potassium chloride 0.5, the g of potassium dihydrogen phosphate 1.0, ferrous sulfate
0.02 g, adds water to 1000mL.121 DEG C of Jing, 0.1MPa sterilizing 20min process, cools down standby after liquid seed culture medium preparation；
（3）The Tween-80 that 15mL mass concentrations are 1.0% is aseptically added in eggplant-shape bottle（Sterilising conditions：115℃、
0.068MPa, 30 min）, then lawn in eggplant-shape bottle is scraped and is seeded in aforesaid liquid seed culture medium, 28 DEG C, 160r/
Min constant-temperature table culture 24h, obtain final product thick wall spore Pu Keniya bacterium bacterial classification suspension.
Detection suspension clump count is 4.7 × 107cfu/mL.Detection method is dilution plate rubbing method, detects culture medium
For PDA culture medium.
3 liquid fermentations
Thick wall spore Pu Keniya bacteria liquid fermentation mediums are prepared, liquid fermentation medium formula is：The g of corn flour 25, sucrose 10
G, the g of bean cake powder 10, the g of sodium chloride 5, the g of magnesium sulfate 0.5, the g of potassium chloride 0.5, ferrous sulfate 0.01g, add water to
1000mL.With 1.0mol/L HCl solutions and 2.0mol/L NaOH solutions liquid fermentation medium is adjusted to pH6.0 ± 0.2, with
The formation of induced meristem spore.Dispense after the completion of preparation to 250mL triangular flasks, liquid amount is 100mL, sterilising conditions are：121
DEG C, 0.1MPa, 30min.
Cultured thick wall spore Pu Keniya bacterium bacterial classification suspension is seeded to by 10% inoculum concentration on superclean bench
In liquid fermentation medium, in constant-temperature table shaken cultivation, condition of culture is：Rotating speed 160r/min, 28 DEG C of cultivation temperature is sent out
Ferment 144-168h.
4 post processings
Zymotic fluid after fermentation ends is filtered using sterile gauze, thick wall spore Pu Keniya bacterium mycelium are removed, filtrate adopts
Flat board gradient dilution rubbing method detects spore amount, and detection culture medium is PDA, and final spore amount is 25.45 × 108cfu/mL。
The liquid fermentation of embodiment 2 produces thick wall spore Pu Keniya bacterium conidiums
Comprise the steps：
Step 1-2 is with embodiment 1.
3 liquid fermentations
Thick wall spore Pu Keniya bacteria liquid fermentation mediums are prepared, liquid fermentation medium formula is：The g of corn flour 35, sucrose 5
G, the g of bean cake powder 5, the g of sodium chloride 1, the g of magnesium sulfate 0.1, the g of potassium chloride 0.1, the g of ferrous sulfate 0.01, add water to
1000mL.With 1.0mol/L HCl solutions and 2.0mol/L NaOH solutions liquid fermentation medium is adjusted to pH6.0 ± 0.2, with
The formation of induced meristem spore.Dispense after the completion of preparation to 250mL triangular flasks, liquid amount is 100mL, sterilising conditions are：121
DEG C, 0.1MPa, 30min.
Cultured thick wall spore Pu Keniya bacterium bacterial classification suspension is seeded to by 10% inoculum concentration on superclean bench
In fermentation medium, in constant-temperature table shaken cultivation, condition of culture is：Rotating speed 160r/min, 28 DEG C of cultivation temperature, fermentation
144-168h。
4 post processings
Zymotic fluid after fermentation ends is filtered using sterile gauze, thick wall spore Pu Keniya bacterium mycelium are removed, filtrate adopts
Flat board gradient dilution rubbing method detects spore amount, and detection culture medium is PDA, and final spore amount is 16.01 × 108cfu/mL。
The liquid fermentation of embodiment 3 produces thick wall spore Pu Keniya bacterium conidiums
Comprise the steps：
Step 1-2 is with embodiment 1.
3 liquid fermentations
Thick wall spore Pu Keniya bacteria liquid fermentation mediums are prepared, liquid fermentation medium formula is：The g of corn flour 15, sucrose 20
G, the g of bean cake powder 15, the g of sodium chloride 10, the g of magnesium sulfate 1.0, the g of potassium chloride 1.0, the g of ferrous sulfate 0.01, add water to
1000mL.With 1.0mol/L HCl solutions and 2.0mol/L NaOH solutions liquid fermentation medium is adjusted to pH6.0 ± 0.2, with
The formation of induced meristem spore.Dispense after the completion of preparation to 250mL triangular flasks, liquid amount is 100mL, sterilising conditions are：121
DEG C, 0.1MPa, 30min.
Cultured thick wall spore Pu Keniya bacterium bacterial classification suspension is seeded to by 10% inoculum concentration on superclean bench
In fermentation medium, in constant-temperature table shaken cultivation, condition of culture is：Rotating speed 160r/min, 28 DEG C of cultivation temperature, fermentation
144-168h。
4 post processings
Zymotic fluid after fermentation ends is filtered using sterile gauze, thick wall spore Pu Keniya bacterium mycelium are removed, filtrate adopts
Flat board gradient dilution rubbing method detects spore amount, and detection culture medium is PDA, and final spore amount is 18.15 × 108cfu/mL。
The liquid fermentation of embodiment 4 produces thick wall spore Pu Keniya bacterium conidiums
Comprise the steps：
Step 1-2 is with embodiment 1.
3 liquid fermentations
Thick wall spore Pu Keniya bacteria liquid fermentation mediums are prepared, liquid fermentation medium formula is：The g of corn flour 30, sucrose 8
G, the g of bean cake powder 12, the g of sodium chloride 8, the g of magnesium sulfate 0.7, the g of potassium chloride 0.7, the g of ferrous sulfate 0.01, add water to
1000mL.With 1.0mol/L HCl solutions and 2.0mol/L NaOH solutions liquid fermentation medium is adjusted to pH6.0 ± 0.2, with
The formation of induced meristem spore.Dispense after the completion of preparation to 250mL triangular flasks, liquid amount is 100mL, sterilising conditions are：121
DEG C, 0.1MPa, 30min.
Cultured thick wall spore Pu Keniya bacterium bacterial classification suspension is seeded to by 10% inoculum concentration on superclean bench
In fermentation medium, in constant-temperature table shaken cultivation, condition of culture is：Rotating speed 160r/min, 28 DEG C of cultivation temperature, fermentation
144-168h。
4 post processings
Zymotic fluid after fermentation ends is filtered using sterile gauze, thick wall spore Pu Keniya bacterium mycelium are removed, filtrate adopts
Flat board gradient dilution rubbing method detects spore amount, and detection culture medium is PDA, and final spore amount is 20.35 × 108cfu/mL。
The liquid fermentation of embodiment 5 produces thick wall spore Pu Keniya bacterium conidiums
Comprise the steps：
Step 1-2 is with embodiment 1.
3 liquid fermentations
Thick wall spore Pu Keniya bacteria liquid fermentation mediums are prepared, liquid fermentation medium formula is：The g of corn flour 20, sucrose 15
G, the g of bean cake powder 8, the g of sodium chloride 3, the g of magnesium sulfate 0.3, the g of potassium chloride 0.3, the g of ferrous sulfate 0.01, add water to
1000mL.With 1.0mol/L HCl solutions and 2.0mol/L NaOH solutions liquid fermentation medium is adjusted to pH6.0 ± 0.2, with
The formation of induced meristem spore.Dispense after the completion of preparation to 250mL triangular flasks, liquid amount is 100mL.Sterilising conditions are：121
DEG C, 0.1MPa, 30min.
Cultured thick wall spore Pu Keniya bacterium bacterial classification suspension is seeded to by 10% inoculum concentration on superclean bench
In fermentation medium, in constant-temperature table shaken cultivation, condition of culture is：Rotating speed 160r/min, 28 DEG C of cultivation temperature, fermentation
144-168h。
4 post processings
Zymotic fluid after fermentation ends is filtered using sterile gauze, thick wall spore Pu Keniya bacterium mycelium are removed, filtrate adopts
Flat board gradient dilution rubbing method detects spore amount, and detection culture medium is PDA, and final spore amount is 19.85 × 108cfu/mL。
Although above with a general description of the specific embodiments the present invention is described in detail,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, without departing from theon the basis of the spirit of the present invention these modifications or improvements, belong to the scope of protection of present invention.
Claims (8)
1. a kind of thick wall spore Pu Keniya bacteria liquids ferment conidiiferous method, it is characterised in that including thick wall spore is general can
The step of Buddhist nun Asia bacterium actication of culture, the preparation of bacterial classification spore suspension and liquid fermentation produce spore；
Wherein, the liquid fermentation medium formula used in the liquid fermentation product spore step is：Corn flour 15-35 g, sugarcane
Sugared 5-20 g, bean cake powder 5-15 g, sodium chloride 1-10 the g, -1.0g of magnesium sulfate 0.1, potassium chloride 0.1-1.0 g, ferrous sulfate
0.01 g, adds water to 1000mL.
2. method according to claim 1, it is characterised in that the liquid fermentation produces liquid fermentation medium in spore step
Fill a prescription and be：Corn flour 20-30 g, sucrose 8-15 g, bean cake powder 8-12 g, sodium chloride 3-8 g, magnesium sulfate 0.3-0.8 g, chlorination
Potassium 0.3-0.8 g, the g of ferrous sulfate 0.01, add water to 1000mL.
3. method according to claim 1, it is characterised in that the liquid fermentation produces liquid fermentation medium in spore step
Fill a prescription and be：The g of corn flour 25, the g of sucrose 10, the g of bean cake powder 10, the g of sodium chloride 5, the g of magnesium sulfate 0.5, the g of potassium chloride 0.5, sulfuric acid
0.01 g of ferrous iron, adds water to 1000mL.
4. the method according to any one of claim 1 or 3, it is characterised in that the liquid fermentation produces liquid in spore step
Fermentation medium is adjusted to pH6.0 ± 0.2.
5. method according to claim 1, it is characterised in that the thick wall spore Pu Keniya bacterium actication of culture steps are：
Thick wall spore Pu Keniya bacterium bacterial classifications are seeded in into activation culture 5d on PDA inclined-planes, cultivation temperature is 28 DEG C.
6. method according to claim 1, it is characterised in that the preparation method of the bacterial classification spore bacteria suspension is：
A. the thick wall spore Pu Keniya bacterium strain transfer Jing after slant activation is cultivated into eggplant-shape bottle, 28 DEG C of cultivation temperature, is trained
Foster time 5-7d；
B. aseptically in the eggplant-shape bottle add 15mL mass concentrations be 1.0% Tween-80, then lawn is scraped
Under be seeded to liquid seed culture medium, 28 DEG C, 160r/min constant-temperature table culture 24h obtain final product bacterial classification spore suspension；
Wherein, the formula of liquid seed culture medium is in step b：The g of sucrose 30, the g of dusty yeast 0.5, the g of sodium nitrate 3.0, sulphur
The g of sour magnesium 0.5, the g of potassium chloride 0.5, the g of potassium dihydrogen phosphate 1.0, the g of ferrous sulfate 0.02, add water to 1000mL.
7. method according to claim 1, it is characterised in that the step of liquid fermentation produces spore be：By the bacterial classification
Spore bacteria suspension is seeded in the liquid fermentation medium by 10% inoculum concentration, in constant-temperature table shaken cultivation, constant-temperature table
Shaken cultivation condition is：Shaking speed 160r/min, 28 DEG C of cultivation temperature, incubation time are 144-168h.
8. the method according to any one in claim 1,2,3,5,6,7, it is characterised in that for liquid fermentation thickness wall
The conidial thick wall spore Pu Keniya bacterial strains of spore Pu Keniya bacterium are purchased from Chinese agriculture Culture Collection, and preservation is compiled
Number be ACCC 30601.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611262188.2A CN106591212A (en) | 2016-12-30 | 2016-12-30 | Method for producing conidia by Pochonia chlamydosporia liquid fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611262188.2A CN106591212A (en) | 2016-12-30 | 2016-12-30 | Method for producing conidia by Pochonia chlamydosporia liquid fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106591212A true CN106591212A (en) | 2017-04-26 |
Family
ID=58581814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611262188.2A Pending CN106591212A (en) | 2016-12-30 | 2016-12-30 | Method for producing conidia by Pochonia chlamydosporia liquid fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106591212A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107955802A (en) * | 2017-12-05 | 2018-04-24 | 福建农林大学 | A kind of method of thickness wall spore Pu Keniya bacteria solid fermentations production spore |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513975A (en) * | 2003-06-03 | 2004-07-21 | 云南大学 | Method of producing large quantity thick wall spore by thick wall spore verticillium liquid fermentation |
| CN101263221A (en) * | 2005-09-13 | 2008-09-10 | 埃武拉大学 | Liquid media for chlamydospore production of the fungus pochonia chlamydsporia |
| CN103172447A (en) * | 2012-12-21 | 2013-06-26 | 山东金正大生态工程股份有限公司 | Special controlled-release fertilizer for biological control of soil-borne diseases of watermelons, and preparation method and application of fertilizer |
| CN104789517A (en) * | 2015-05-07 | 2015-07-22 | 佛山市海天调味食品股份有限公司 | Method for increasing amount of spores |
-
2016
- 2016-12-30 CN CN201611262188.2A patent/CN106591212A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513975A (en) * | 2003-06-03 | 2004-07-21 | 云南大学 | Method of producing large quantity thick wall spore by thick wall spore verticillium liquid fermentation |
| CN101263221A (en) * | 2005-09-13 | 2008-09-10 | 埃武拉大学 | Liquid media for chlamydospore production of the fungus pochonia chlamydsporia |
| CN103172447A (en) * | 2012-12-21 | 2013-06-26 | 山东金正大生态工程股份有限公司 | Special controlled-release fertilizer for biological control of soil-borne diseases of watermelons, and preparation method and application of fertilizer |
| CN104789517A (en) * | 2015-05-07 | 2015-07-22 | 佛山市海天调味食品股份有限公司 | Method for increasing amount of spores |
Non-Patent Citations (7)
| Title |
|---|
| LI GAO ET AL.: "Use of a novel two-stage cultivation method to determine the effects of environmental factors on the growth and sporulation of several biocontrol fungi", 《MYCOSCIENCE》 * |
| LING-LING XU ET AL.: "A novel method for promoting conidial production by a nematophagous fungus,Pochonia chlamydosporiaAS6.8", 《WORLD J MICROBIOL BIOTECHNOL》 * |
| 刘春秀等: "厚壁轮枝菌摇瓶液体发酵培养的研究", 《林业科学研究》 * |
| 张国珍: "《植物病原微生物学》", 30 September 2016, 中国农业大学出版社 * |
| 王曦茁等: "厚垣普可尼亚菌液体发酵动力学", 《林业科学》 * |
| 石妍等: "两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件", 《安徽农业科学》 * |
| 高利: "液体与固体培养几种生防真菌的营养研究", 《中国生物防治》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107955802A (en) * | 2017-12-05 | 2018-04-24 | 福建农林大学 | A kind of method of thickness wall spore Pu Keniya bacteria solid fermentations production spore |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104371934B (en) | A kind of trichoderma reesei mutant strain and its application | |
| CN106676040A (en) | Streptomyces griseoplanus, application thereof and microbial agent | |
| CN103146617A (en) | Bacillus subtilis and culture method and application thereof | |
| CN104928202A (en) | Fermentation culture method of bacillus | |
| CN102533579A (en) | Culture medium for producing spores, and optimizing process and application thereof | |
| US20240228391A1 (en) | Exiguobacterium indicum and application thereof in synthesis of nano-selenium | |
| CN103146624A (en) | Mixed liquid fermentation process of three plants of bacillus licheniformis | |
| CN108865934B (en) | Bacillus safensis HMD9204 and microbial inoculum and application thereof | |
| CN103525748B (en) | The chlamydosporic production method of trichoderma harziarum | |
| CN104651339B (en) | The culture medium and its fermentation process of microvesicle Pseudomonas fermenting and producing algin catenase | |
| CN108865938A (en) | A kind of bacillus stearothermophilus of high yield gemma | |
| CN111733119A (en) | High-density fermentation method of bacillus subtilis | |
| CN102127515A (en) | Screening and application of L-proline high-producing Brevundimonas sp. (JNPP-1) | |
| CN104805029B (en) | A kind of preparation method of fertilizer | |
| CN106591212A (en) | Method for producing conidia by Pochonia chlamydosporia liquid fermentation | |
| CN106834177A (en) | One plant of cud bacterium and its application | |
| CN106701655A (en) | Method of producing pochonia chlamydosporia spores by virtue of liquid-solid double phase fermentation | |
| CN114107097B (en) | Solid fermentation process of streptomyces avermitilis active spores | |
| CN110699352A (en) | Breeding method of trichoderma harzianum high-yield strain for preventing and controlling potato root rot | |
| CN102344895B (en) | Method for screening sodium chloride-resistant bacillus licheniformis | |
| Gouli et al. | Productivity of different species of entomopathogenic fungi based on one type of technology. | |
| CN101861796B (en) | Method for culturing amanita pantherina by using waste distillage after fermentation of coloured rice | |
| CN107177525A (en) | A kind of complex micro organism fungicide and its preparation method and application | |
| CN105018409A (en) | Methyl nutritional type bacillus spore-production fermentation medium and culture method thereof | |
| CN103947682A (en) | Preparation technology of bacillus licheniformis inoculant capable of resisting strawberry replant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Applicant after: Taigu ecological science and technology group Limited by Share Ltd Address before: 410205 floor 14, A, Lulu business center, No. 199 Lulong Road, Changsha high tech Zone, Hunan. Applicant before: Hunan Taigu Biotechnology Co., Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170426 |