CN106591137A - Heterogloea sp. culture medium composition - Google Patents
Heterogloea sp. culture medium composition Download PDFInfo
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- CN106591137A CN106591137A CN201611258652.0A CN201611258652A CN106591137A CN 106591137 A CN106591137 A CN 106591137A CN 201611258652 A CN201611258652 A CN 201611258652A CN 106591137 A CN106591137 A CN 106591137A
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- culture medium
- different glue
- heterogloea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention discloses a heterogloea sp. culture medium composition. The heterogloea sp. culture medium composition is characterized by being prepared from the following components: KNO3: 300-400 mg/L, KH2PO4: 10mg/L, FeSO4.7H2O: 2.5mg/L, Na2EDTA: 10mg/L, MnSO4: 0.25g/L, VB1: 6mg/L, VB12: 50 [mu] g/L, an additive: 0.5-3 g/L, and a plant growth regulator composed of 6-BA and naphthylacetic acid. When the heterogloea sp. culture medium composition is used for culturing heterogloea sp, the heterogloea sp. has the features of high growth velocity and large cell density.
Description
Technical field
The present invention relates to culture medium technical field, more particularly to a kind of different glue algae culture medium compositionss.
Background technology
Different glue algae (Heterogloea sp.) category Xanthophyta, Heterocapsaceae, different glue Trentepohlia, are that marine products Bivalve is manually educated
Seedling stage good biological feed, it is wide in the artificial breeding of Concha Ostreae, Sinonovacula constricta, Ruditapes philippinarum, Chlamys nobilis, Tegillarca granosa etc.
General application.Used as bait micro-algae, which has following features：(1) it is unicellular, it is individual small, it is evenly distributed in suspended state；(2)
It is stronger to the adaptability of water temperature, illumination, proportion；(3) breed very fast, algae solution concentration is higher, has stronger to harmful animal protozoacide
Rejection ability；(4) Zhou Nianjun can be cultivated, and cultural method is easy.It is therefore preferable that go out the high-yield culture medium of different glue algae, it is big to which
It is significant that scale evaluation is used for actual production.
How applicant one come the speed of growth and culture density for improving different glue algae by different glue algae culture medium compositionss
Directly it is devoted to the direction studied.
The content of the invention
The invention aims to the one kind for solving the not enough of above-mentioned technology and designing can make different glue algae fast-growth
With a kind of different glue algae culture medium compositionss for increasing culture density.
A kind of different glue algae culture medium compositionss designed by the present invention, including following component：
KNO3：300-400mg/L；KH2PO4：10mg/L；FeSO4·7H2O：2.5mg/L；Na2EDTA：10mg/L；
MnSO4：0.25g/L：VB1：6mg/L；VB12：50ug/L；Additive：0.5-3g/L；Plant growing made by 6-BA and naphthalene acetic acid
Regulator.
Further preferably, the 6-BA：0.1-1mg/L；Naphthalene acetic acid：0.1-1mg/L.
Further preferably, the additive is glycine.
Further preferably, also increase NaNO including being raised through for N/P ratios3Content.
A kind of different glue algae culture medium compositionss designed by the present invention, beneficial to frustule fast-growth, the different glue turned out
Algae cell density is big, especially because NaNO3Content increases and improves N/P ratios；Additive：Glycine 0.5-3g/L；Plant
Growth regulator：6-BA 0.1-1mg/L, naphthalene acetic acid 0.1-1mg/L, rationally, the fast-growth for promoting different glue algae is numerous for formula
Grow, algae cell density increase is suitable to large-scale production.
Description of the drawings
Fig. 1 is the comparative test result of embodiments of the invention 1, embodiment 2, embodiment 3 and MAV culture medium.
Specific embodiment
Embodiment of the lower mask body with reference to Fig. 1, is described in detail to the different glue algae culture medium that the present invention is provided.
Embodiment one：
A kind of different glue algae culture medium compositionss, including following component：KNO3：300mg/L；KH2PO4：10mg/L；FeSO4·
7H2O：2.5mg/L；Na2EDTA：10mg/L；MnSO4：0.25g/L：VB1：6mg/L；VB12：50ug/L；Glycine：1g/L；6-
BA：0.5mg/L；Naphthalene acetic acid：0.5mg/L.
Sea water after being filtered with 1000ml configures the different glue algae culture medium compositionss of said ratio, and daily illumination 12 is little
When, intensity of illumination is about 50 μm of ol/ (m2S), incubator temperature control detects algae cell density daily at 25 DEG C.
Embodiment two：
A kind of different glue algae culture medium compositionss, including following component：KNO3：300mg/L；KH2PO4：10mg/L；FeSO4·
7H2O：2.5mg/L；Na2EDTA：10mg/L；MnSO4：0.25g/L：VB1：6mg/L；VB12：50ug/L；Glycine：3g/L；6-
BA：1mg/L；Naphthalene acetic acid：1mg/L.
Sea water after being filtered with 1000ml configures the different glue algae culture medium compositionss of said ratio, and daily illumination 12 is little
When, intensity of illumination is about 50 μm of ol/ (m2S), incubator temperature control detects algae cell density daily at 25 DEG C.
Embodiment three：
A kind of different glue algae culture medium compositionss, including following component：KNO3：350mg/L；KH2PO4：10mg/L；FeSO4·
7H2O：2.5mg/L；Na2EDTA：10mg/L；MnSO4：0.25g/L：VB1：6mg/L；VB12：50ug/L；Glycine：0.5g/L；
6-BA：0.1mg/L；Naphthalene acetic acid：0.1mg/L.
Using MAV culture medium as control medium, the different glue algae of trophophase of taking the logarithm is carried out in being inoculated into the culture medium
Culture, daily illumination 12 hours, intensity of illumination are about 50 μm of ol/ (m2S), incubator temperature control is detected daily at 25 DEG C
Algae cell density.
Following table is：The cell concentration contrast table of the different glue algae of different culture media culture
Different glue algae growth and breeding can more quickly be made using MAV culture medium using culture medium ratio provided by the present invention,
From the optimization different glue algae of wild Oryza species culture, its highest cell concentration is far above control MAV culture medium, about high by 19%.Thus
Illustrate that the different glue algae culture medium of present invention offer has significantly culture advantage, be conducive to the fast-growth of different glue algae.
Schematically specific embodiment of the invention is the foregoing is only, the scope of the present invention is not limited to.It is any
Those skilled in the art, done equivalent variations and modification on the premise of the design without departing from the present invention and principle,
The scope of protection of the invention should be belonged to.
Claims (4)
1. a kind of different glue algae culture medium compositionss, it is characterised in that：Including following component：
KNO3：300-400mg/L；KH2PO4：10mg/L；FeSO4·7H2O：2.5mg/L；Na2EDTA：10mg/L；MnSO4：
0.25g/L：VB1：6mg/L；VB12：50ug/L；Additive：0.5-3g/L；Plant growth regulating made by 6-BA and naphthalene acetic acid
Agent.
2. a kind of different glue algae culture medium compositionss according to claim 1, it is characterised in that：The 6-BA：0.1-1mg/L；
Naphthalene acetic acid：0.1-1mg/L.
3. a kind of different glue algae culture medium compositionss according to claim 1, it is characterised in that：The additive is glycine.
4. a kind of different glue algae culture medium compositionss according to claim 1, it is characterised in that：Also including the rising of N/P ratios
By increasing NaNO3Content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611258652.0A CN106591137A (en) | 2016-12-30 | 2016-12-30 | Heterogloea sp. culture medium composition |
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| CN201611258652.0A CN106591137A (en) | 2016-12-30 | 2016-12-30 | Heterogloea sp. culture medium composition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172096A (en) * | 2020-04-11 | 2020-05-19 | 杭州富阳君晓农业科技有限公司 | Production process for high-density culture of heteroglena |
| CN111394254A (en) * | 2020-04-21 | 2020-07-10 | 杭州富阳君晓农业科技有限公司 | Preparation method and application of isopulegolic algae powder |
Citations (3)
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| CN101803600A (en) * | 2009-02-13 | 2010-08-18 | 云南爱尔发生物技术有限公司 | Haematococcus pluvialis cell growth promoting agent and use method thereof |
| CN104130945A (en) * | 2014-08-08 | 2014-11-05 | 临沂大学 | Culture medium and culture method for high-density culture of heterogloea.sp |
| CN104560722A (en) * | 2015-01-30 | 2015-04-29 | 宁波浮田生物技术有限公司 | Dunaliella salina culture medium |
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2016
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803600A (en) * | 2009-02-13 | 2010-08-18 | 云南爱尔发生物技术有限公司 | Haematococcus pluvialis cell growth promoting agent and use method thereof |
| CN104130945A (en) * | 2014-08-08 | 2014-11-05 | 临沂大学 | Culture medium and culture method for high-density culture of heterogloea.sp |
| CN104560722A (en) * | 2015-01-30 | 2015-04-29 | 宁波浮田生物技术有限公司 | Dunaliella salina culture medium |
Non-Patent Citations (5)
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| 刘世名等: "植物激素在小球藻异养培养中的作用", 《植物学通报》 * |
| 季祥等: "《生物质能源及废物利用新技术》", 31 December 2012, 吉林大学出版社 * |
| 张桂和等: "植物激素促进海洋微藻生长的效应", 《中国科协2004年学术年会》 * |
| 王家福: "《花卉组织培养与快繁技术》", 31 January 2006, 中国林业出版社 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172096A (en) * | 2020-04-11 | 2020-05-19 | 杭州富阳君晓农业科技有限公司 | Production process for high-density culture of heteroglena |
| CN111172096B (en) * | 2020-04-11 | 2022-04-12 | 杭州渔森农业技术开发有限公司 | Production process for high-density culture of heteroglena |
| CN111394254A (en) * | 2020-04-21 | 2020-07-10 | 杭州富阳君晓农业科技有限公司 | Preparation method and application of isopulegolic algae powder |
| CN111394254B (en) * | 2020-04-21 | 2022-11-25 | 杨凌海思生物科技有限公司 | Preparation method and application of isopulva powder |
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