CN106581089A - Preparation method of folium ginkgo extract - Google Patents
Preparation method of folium ginkgo extract Download PDFInfo
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- CN106581089A CN106581089A CN201611231845.7A CN201611231845A CN106581089A CN 106581089 A CN106581089 A CN 106581089A CN 201611231845 A CN201611231845 A CN 201611231845A CN 106581089 A CN106581089 A CN 106581089A
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- ethanol
- folium ginkgo
- ginkgo biloba
- ginkgo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a preparation method of folium ginkgo extract. The preparation method comprises following steps: (1), pretreatment: fresh folium ginkgo is taken, washed, air-dried or braked and smashed until the leaf area is not larger than 20 mm<2>; (2), extraction: a 40v%-90v% ethanol solution which is 1-5 times the weight of the folium ginkgo raw material prepared in the step (1) is added to the folium ginkgo raw material, a mixed solution is heated to 50-80 DEG C, kept at the temperature for 1-4 h and then filtered, filter residues are washed with a 10v%-99v% ethanol solution which is 0.5-1 time the weight of the filter residues, the washing solutions are combined, and a first ethanol extracting solution is obtained; (3), the first ethanol extracting solution is subjected to refined filtration with a 200-mesh screen and suction filtration, ethanol is recovered, the obtained solution is concentrated to dryness, and the folium ginkgo extract is obtained. According to the preparation method of the folium ginkgo extract, the ethanol solutions are adopted for mild extraction treatment, selectivity for medicinal composition in the folium ginkgo is good, and the extraction efficiency is high.
Description
Technical field
The present invention relates to a kind of Chinese crude drug processing and concocting method, more particularly to a kind of preparation method of Ginkgo Biloba Extract,
Belong to traditional Chinese medicine preparation for processing.
Background technology
Semen Ginkgo is one of most ancient gymnosperm for carrying over after quaternary glacier motion, has the laudatory title of living fossil.
Folium Ginkgo, also known as moth leaf, duck bottom sediment, are Ginkgoaceae plant Ginkgo bilobaGinkgo biloba L.Dried leaves.Autumn
Harvest when Ji Yeshang is green, be dried in time.The many fold of Folium Ginkgo are broken, and complete person is in fan-shaped, long 3～12cm, wide 5～15cm.
Yellow green or sundown, in irregular wavy bending, some centres are recessed, and deep person up to leaf length 4/5 for upper limb.
Folium Ginkgo body is light, feeble QI, mildly bitter flavor.GUIXIN, lung meridian.Indication：Astringe the lung, relieving asthma, blood circulation promoting and blood stasis dispelling, pain relieving.With
In cough and asthma due to lung deficiency；Coronary heart disease, angina pectoriss, hyperlipidemia.
Extracting method of the prior art mainly includes following several method：
1. organic solvent extraction：Conventional ethanol, acetone, aqueous solution etc. are extracted.
2. steam distillation：Mainly the volatile oil in Semen Ginkgo can be had using vapor distillation Folium Ginkgo
The extraction of effect.
3. supercritical extraction method：Using the supercritical properties of CO2, the volatile oil in fluid extraction Folium Ginkgo into
Point.
4. ultrasonic extraction：The cellularity of Folium Ginkgo is destroyed by the shear action of ultrasound wave so that in Folium Ginkgo
Nutritional labeling discharge.
Above-mentioned extracting method has technology single, is easily processed the problem of local energy accumulation, causes in extraction process
The active component of the Folium Ginkgo that extraction is obtained significantly loses, and then the using effect of Folium Ginkgo is not good.Or, occur carrying
The big problem of energy consumption during taking so that extracting method is difficult to industrial applications, can only be in the environment of laboratory
Reappear, with greatly restricted.
The content of the invention
It is an object of the invention to overcome the shortcomings of Folium Ginkgo extracting method in prior art, there is provided a kind of Folium Ginkgo is extracted
The high Ginkgo Biloba Extract preparation method of rate.
In order to realize foregoing invention purpose, the invention provides technical scheme below：
A kind of Ginkgo Biloba Extract preparation method, comprises the following steps：
（1）Pretreatment：Fresh Folium Ginkgo is taken, is cleaned, is dried or dry, smashed to blade area and be not more than 20 square millimeters.
（2）Extract：Semen Ginkgo leaf raw material prepared by step 1, adds the 40-90v% ethanol solution of 1-5 times of weight, is heated to
50-80 DEG C of holding 1-4 hour, filters, and filtering residue is washed with the 10-99v% ethanol solution of 0.5-1 times of weight, merges wash solution,
As the first ethanol extract.
（3）By the first ethanol extract, with 200 eye mesh screen fine straining, sucking filtration, ethanol is reclaimed, is concentrated to dryness, is obtained Folium Ginkgo
Extract.
Further, step 1, in drying course, temperature is less than 85 DEG C.
Further, step 1, smashes to Ginkgo Biloba Leaves Extract Tablets area and is not more than 18 square millimeters.
Further, step 2, adds 2-4 times of ethanol solution to be extracted.
Further, step 2, the ethanol solution used in extraction process are the ethanol solution of 50-85v%.
Further, step 2, filters the screen cloth that the screen cloth for using is 40-80 mesh.
Further, step 2, residue washing use the ethanol solution of 20-50v%.
Further, step 3, reclaim ethanol in raw material more than 70% ethanol be recycled after, it is starting to be concentrated to dryness plus
Work.
Further, step 3, realizes being concentrated to dryness using concentrating under reduced pressure, and control thickening temperature is less than 75 DEG C.
Compared with prior art, beneficial effects of the present invention：
1. the method for preparing extractive of the Folium Ginkgo of the present invention, carries out gentle extraction process using ethanol solution, for Semen Ginkgo
Active ingredient selectivity in leaf is good, and extraction efficiency is high.
2. extracting method of the present invention simply uses ethanol solution, and for the concentration ratio of ethanol solution is controlled,
The selection control of the special component in Folium Ginkgo is realized, the easy control of temperature in extraction process is convenient to adjust.
3. simple production process of the present invention, easy, few with step, and the characteristics of simple to operate, Extraction solvent can be reclaimed
Utilize, with it is efficient the characteristics of.
Specific embodiment
With reference to test example and specific embodiment, the present invention is described in further detail.But this should not be understood
Scope for above-mentioned theme of the invention is only limitted to below example, and all technologies realized based on present invention belong to this
The scope of invention.In the present invention, not specified percentage ratio is weight percentage.
Embodiment 1
It is prepared by Ginkgo Biloba Extract
Take fresh Folium Ginkgo to clean, dry, smash to monolithic Semen Ginkgo leaf area less than 20 square millimeters, be transferred to brown, wide-mouth
In bottle；Add the ethanol solution that volumetric concentration is 60%, submergence Folium Ginkgo that wide mouthed bottle is transferred in 70-80 DEG C of water-bath,
Heating in water bath extracts 4h, and variable interval stirring makes Folium Ginkgo and ethanol solution be sufficiently mixed uniformly, first removes big portion with filtered through gauze
Point Folium Ginkgo, then washs the Folium Ginkgo residue on gauze with the 30v% ethanol solution of 600ml, and filtrate is with 200 eye mesh screen fine straining
Once, then sucking filtration is carried out with the buchner funnel with micronic paper, using 3-5 filter paper, collect filtrate and abandon filtering residue；This filtrate
Decompression recycling ethanol, is then transferred in enrichment facility, is concentrated, and must concentrate filtrate；Filtrate will be concentrated in 60-65 DEG C of vacuum
It is dried, obtains dry Ginkgo Biloba Extract, the extract includes flavonoid and lactone.The yield of the extract
15.36%.
Embodiment 2
It is prepared by Ginkgo Biloba Extract
Take fresh Folium Ginkgo to clean, dry, smash to monolithic Semen Ginkgo leaf area less than 18 square millimeters, be transferred to brown, wide-mouth
In bottle；Add the ethanol solution that volumetric concentration is 50%, submergence Folium Ginkgo that wide mouthed bottle is transferred in 75-80 DEG C of water-bath,
Heating in water bath extracts 5h, and variable interval stirring makes Folium Ginkgo and ethanol solution be sufficiently mixed uniformly, first removes big portion with filtered through gauze
Point Folium Ginkgo, then washs the Folium Ginkgo residue on gauze with the 30v% ethanol solution of 500ml, and filtrate is with 200 eye mesh screen fine straining
Once, then sucking filtration is carried out with the buchner funnel with micronic paper, using 4 filter paper, collect filtrate and abandon filtering residue；This filtrate subtracts
Receipts ethanol is pushed back, is then transferred in enrichment facility, is concentrated, filtrate must be concentrated；Concentration filtrate is done in 62-68 DEG C of vacuum
It is dry, dry Ginkgo Biloba Extract is obtained, the extract includes flavonoid and lactone.The yield of the extract
13.14%.
Embodiment 3
It is prepared by Ginkgo Biloba Extract
Take fresh Folium Ginkgo to clean, dry, smash to monolithic Semen Ginkgo leaf area less than 18 square millimeters, be transferred to brown, wide-mouth
In bottle；Add the ethanol solution that volumetric concentration is 30%, about the 2 of Semen Ginkgo leaf raw material times weight, submergence Folium Ginkgo, by wide mouthed bottle
It is transferred in 76-82 DEG C of water-bath, heating in water bath extraction 5h, variable interval stirring make Folium Ginkgo and ethanol solution be sufficiently mixed
It is even, first most of Folium Ginkgo is removed with filtered through gauze, it is then residual with the Folium Ginkgo that the 40v% ethanol solution of 550ml is washed on gauze
Slag, filtrate once, then carry out sucking filtration with the buchner funnel with micronic paper with 200 eye mesh screen fine straining, using 4 filter paper,
Collect filtrate and abandon filtering residue；This decompression filtrate recycling ethanol, is then transferred in enrichment facility, is concentrated, and must concentrate filtrate；Will
Concentration filtrate is vacuum dried at 62-68 DEG C, obtains dry Ginkgo Biloba Extract, and the extract includes flavonoid and interior
Ester.The yield 16.28% of the extract.
Embodiment 4
It is prepared by Ginkgo Biloba Extract
Take fresh Folium Ginkgo to clean, dry, smash to monolithic Semen Ginkgo leaf area less than 20 square millimeters, be transferred to brown, wide-mouth
In bottle；The ethanol solution that volumetric concentration is 80% is added, equivalent to 2.5 times of weight portions of Folium Ginkgo, submergence Folium Ginkgo, by wide-mouth
Bottle is transferred in 75-80 DEG C of water-bath, and heating in water bath extraction 5h, variable interval stirring are sufficiently mixed Folium Ginkgo and ethanol solution
Uniformly, first most of Folium Ginkgo is removed with filtered through gauze, then wash the Folium Ginkgo on gauze with the 30v% ethanol solution of 500ml
Residue, filtrate once, then carry out sucking filtration with the buchner funnel with micronic paper with 200 eye mesh screen fine straining, are filtered using 5
Paper, collects filtrate and abandons filtering residue；This decompression filtrate recycling ethanol, is then transferred in enrichment facility, is concentrated, and must concentrate filter
Liquid；Concentration filtrate is vacuum dried at 65-70 DEG C, dry Ginkgo Biloba Extract is obtained, the extract includes flavonoid
And lactone.The yield 15.74% of the extract.
Embodiment 5
It is prepared by Ginkgo Biloba Extract
Fresh Folium Ginkgo is taken, is cleaned, 60 DEG C of hot-air seasonings, smashed to blade area and be not more than 20 square millimeters.It is transferred to and carries
Take in pot, add the 90v% ethanol solution of 1.5 times of weight, submergence Semen Ginkgo leaf raw material to be heated to 80 DEG C and kept for 2 hours, filter, filter
Slag is washed with the 10v% ethanol solution of 0.5 times of weight, merges wash solution, as the first ethanol extract.With 200 eye mesh screens essence
Filter, sucking filtration are reclaimed ethanol, are concentrated to dryness, obtain Ginkgo Biloba Extract.The yield 15.14% of the extract.
Embodiment 6
It is prepared by Ginkgo Biloba Extract
Fresh Folium Ginkgo is taken, is cleaned, 75 DEG C of hot-air seasonings, smashed to blade area and be not more than 15 square millimeters.It is transferred to and carries
Take in pot, add the 85v% ethanol solution of 1.5 times of weight, submergence Semen Ginkgo leaf raw material to be heated to 80 DEG C and kept for 2 hours, 40 mesh sieves
Net filtration, filtering residue are washed with the 20v% ethanol solution of 1.2 times of weight, merge wash solution, as the first ethanol extract.With
200 eye mesh screen fine straining, sucking filtration reclaim ethanol and are less than 25v% to ethanol content in solution, and 65-67 DEG C is evaporated to dry, obtains
Ginkgo Biloba Extract.The yield 12.68% of the extract.
Claims (9)
1. a kind of Ginkgo Biloba Extract preparation method, comprises the following steps：
（1）Pretreatment：Fresh Folium Ginkgo is taken, is cleaned, is dried or dry, smashed to blade area and be not more than 20 square millimeters；
（2）Extract：Semen Ginkgo leaf raw material prepared by step 1, adds the 40-90v% ethanol solution of 1-5 times of weight, is heated to 50-
80 DEG C of holding 1-4 hours, filter, and filtering residue is washed with the 10-99v% ethanol solution of 0.5-1 times of weight, merges wash solution, as
First ethanol extract；
（3）By the first ethanol extract, with 200 eye mesh screen fine straining, sucking filtration, ethanol is reclaimed, is concentrated to dryness, obtain Folium Ginkgo extraction
Thing.
2. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 1, temperature does not surpass in drying course
Cross 85 DEG C.
3. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 1, smashes to Ginkgo Biloba Leaves Extract Tablets area
No more than 18 square millimeters.
4. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 2, adds 2-4 times of ethanol molten
Liquid is extracted.
5. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 2, the second used in extraction process
Alcoholic solution is the ethanol solution of 50-85v%.
6. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 2, filtering the screen cloth for using is
The screen cloth of 40-80 mesh.
7. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 2, residue washing are used
The ethanol solution of 20-50v%.
8. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 3, reclaims ethanol into raw material
After more than 70% ethanol is recycled, start the processing being concentrated to dryness.
9. Ginkgo Biloba Extract preparation method as claimed in claim 1, it is characterised in that step 3, is realized using concentrating under reduced pressure dense
Be reduced to it is dry, control thickening temperature be less than 75 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231845.7A CN106581089A (en) | 2016-12-28 | 2016-12-28 | Preparation method of folium ginkgo extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611231845.7A CN106581089A (en) | 2016-12-28 | 2016-12-28 | Preparation method of folium ginkgo extract |
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| Publication Number | Publication Date |
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| CN106581089A true CN106581089A (en) | 2017-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611231845.7A Pending CN106581089A (en) | 2016-12-28 | 2016-12-28 | Preparation method of folium ginkgo extract |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446195A (en) * | 2013-08-22 | 2013-12-18 | 陕西洋县志建药业科技有限公司 | Preparation method of ginkgo biloba extract |
| CN104857035A (en) * | 2015-04-24 | 2015-08-26 | 天益京（天津）生物科技有限公司 | Ginkgo biloba leaf extract preparation method |
-
2016
- 2016-12-28 CN CN201611231845.7A patent/CN106581089A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446195A (en) * | 2013-08-22 | 2013-12-18 | 陕西洋县志建药业科技有限公司 | Preparation method of ginkgo biloba extract |
| CN104857035A (en) * | 2015-04-24 | 2015-08-26 | 天益京（天津）生物科技有限公司 | Ginkgo biloba leaf extract preparation method |
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Application publication date: 20170426 |