CN106578765B - Beverage for relieving body fatigue and preparation method thereof - Google Patents
Beverage for relieving body fatigue and preparation method thereof Download PDFInfo
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- CN106578765B CN106578765B CN201611149403.8A CN201611149403A CN106578765B CN 106578765 B CN106578765 B CN 106578765B CN 201611149403 A CN201611149403 A CN 201611149403A CN 106578765 B CN106578765 B CN 106578765B
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- purified water
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Images
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
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- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/194—Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
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- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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- A—HUMAN NECESSITIES
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention discloses a beverage for relieving body fatigue and a preparation method thereof. The feed is prepared from the following raw materials in parts by weight: 5-10 parts of small molecular organic acid, 5-10 parts of sucrose, 10-20 parts of neoagarotetraose, 5-10 parts of fructooligosaccharide, 10-20 parts of a soft-shelled spirulina, hermatypium, kelp, underclothes and sargassum pallidum compound extract concentrated solution, 2-5 parts of pectin and 300 parts of purified water. The beverage prepared by the invention can relieve the adverse symptoms of body fatigue, small intestine structure damage, intestinal short-chain fatty acid content reduction, flora structure disorder and the like caused by excessive exercise, and has obvious effect on relieving the adverse effect caused by exercise body fatigue. The preparation method is simple and easy to implement, low in preparation cost and suitable for large-scale industrial production.
Description
Technical Field
The invention belongs to the technical field of anti-fatigue and health care, and particularly relates to a beverage for relieving body fatigue and a preparation method thereof.
Background
Modern life rhythm is continuously accelerated, work tasks are gradually increased, a large amount of physical labor or mental labor often causes body fatigue for a long time, activity capacity and work efficiency are reduced, adverse effects can be generated on a digestive system, and normal metabolism and body capacity are further influenced.
The sea is a huge treasury of biological resources, and a large number of animal and plant and algae resources with various physiological activity functions exist. If various natural active substances in the ocean can be fully utilized, the effect of relieving the fatigue symptoms of the organism is realized, and the utilization efficiency of ocean resources can be greatly increased. Meanwhile, the pure natural property of the marine resources can greatly reduce various toxic and side effects brought by the synthetic drugs, achieve the aim of safely, quickly and effectively relieving the fatigue of the organism, improve the exercise capacity and improve the working and learning efficiency.
Disclosure of Invention
The invention aims to provide a beverage for relieving body fatigue.
The invention also aims to provide a preparation method of the beverage for relieving the body fatigue.
A beverage for relieving body fatigue is prepared from the following raw materials in parts by weight: 5-10 parts of small molecular organic acid; 5-10 parts of cane sugar; 10-20 parts of neoagarotetraose; 5-10 parts of fructooligosaccharide; 10-20 parts of soft-shelled algae, spirulina, hermatypium, kelp, underclady algae and sargassum pallidum extract concentrated solution; 2-5 parts of pectin; 300 parts of purified water.
The small molecular organic acid is one or more of malic acid, shikimic acid, citric acid, tartaric acid, glycyrrhizic acid and myristic acid.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, Monostroma arctica, kelp, Neurospora islandica and sargassum pallidum according to the mass ratio of (1-5) to (5-10) to (3-5) to (10-20) to (5-10) to (3-8), adding 10 times of purified water by volume, and homogenizing and pulping; taking 10-20 parts of an extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h, filtering the residue to obtain a slurry, adding 5-10 parts of sucrose, adding 1-3 parts of a lactic acid bacteria agent, fermenting for 24-36h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to 1/3 of the original volume to obtain a compound extract concentrated solution;
(3) according to the mass parts, 5-10 parts of micromolecule organic acid, 10-20 parts of neoagarotetraose, 5-10 parts of fructooligosaccharide, 2-5 parts of pectin and 300 parts of purified water are uniformly mixed with the compound extract concentrated solution, homogenized and dispersed, sterilized instantly at ultrahigh temperature and filled.
The lactobacillus is one or more of Lactobacillus bulgaricus, Lactobacillus plantarum, Streptococcus thermophilus, Lactobacillus acidophilus and Bacillus bifidus.
The ultrahigh-temperature instant sterilization is performed for 6-10s under the conditions of 137-145 ℃.
The invention has the beneficial effects that: the beverage prepared by the invention can relieve the adverse symptoms of body fatigue, small intestine structure damage, intestinal short-chain fatty acid content reduction, flora structure disorder and the like caused by excessive exercise, and has obvious effect on relieving the adverse effect caused by exercise body fatigue. The preparation method is simple and easy to implement, low in preparation cost and suitable for large-scale industrial production.
Drawings
FIG. 1 is a graph comparing the body fatigue indexes of mice in each experimental group.
FIG. 2 is a graph comparing the intestinal integrity indexes of mice in each experimental group.
FIG. 3 is a graph comparing the contents of short-chain fatty acids and SCFAs-producing bacteria in feces of mice in each experimental group.
FIG. 4 is a statistical comparison of fecal flora diversity in mice of each experimental group.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
A beverage for relieving body fatigue comprises the following components in parts by weight: 8 parts of malic acid, 6 parts of sucrose, 20 parts of neoagarotetraose, 5 parts of fructooligosaccharide, 15 parts of a soft-shelled alga, spirulina, hermatypium, kelp, underclothes and sargassum pallidum extract concentrated solution, 3 parts of pectin and 300 parts of purified water.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 2:5:3:10:8:6, adding 10 times of purified water, and homogenizing and pulping; taking 15 parts of an extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h under heat preservation, filtering the dregs to obtain a serous fluid, adding 6 parts of cane sugar, carrying out ultrahigh-temperature instantaneous sterilization, adding 2 parts of lactobacillus plantarum, fermenting for 36h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to an original volume of 1/3, and preparing a compound extract concentrated solution;
(3) 8 parts of malic acid, 20 parts of neoagarotetraose, 5 parts of fructooligosaccharide, 3 parts of pectin and 300 parts of purified water according to the mass parts are uniformly mixed with the compound extraction concentrated solution, homogenized and dispersed, sterilized at 138 ℃ for 8s and filled.
Example 2
A beverage for relieving body fatigue is prepared from the following raw materials in parts by weight: 5 parts of shikimic acid; 5 parts of cane sugar; 10 parts of neoagarotetraose; 5 parts of fructooligosaccharide; 10 parts of soft-shelled algae, spirulina, hermatypium, kelp, underclady algae and sargassum pallidum extract concentrated solution; 2 parts of pectin; 300 parts of purified water.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 1:5:3:11:6:4, adding 10 times of purified water, and homogenizing and pulping; taking 10 parts of extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h, filtering the residue to obtain a slurry, adding 5 parts of sucrose, adding 1 part of lactobacillus bulgaricus microbial inoculum, fermenting for 24h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to an original volume of 1/3 to obtain a compound extract concentrated solution;
(3) uniformly mixing 5 parts of shikimic acid, 10 parts of neoagarotetraose, 5 parts of fructooligosaccharide, 2 parts of pectin and 300 parts of purified water with the compound extraction concentrated solution according to the mass parts, homogenizing and dispersing, sterilizing for 10s at 137 ℃, and filling.
Example 3
A beverage for relieving body fatigue is prepared from the following raw materials in parts by weight: 10 parts of citric acid; 10 parts of cane sugar; 20 parts of neoagarotetraose; 10 parts of fructooligosaccharide; 20 parts of soft-shelled algae, spirulina, hermatypium, kelp, underclady algae and sargassum pallidum extract concentrated solution; 5 parts of pectin; 300 parts of purified water.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 5:10:5:19:10:8, adding 10 times of purified water, and homogenizing and pulping; taking 20 parts of an extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h, filtering the residue to obtain a slurry, adding 10 parts of sucrose, adding 3 parts of streptococcus thermophilus microbial inoculum, fermenting for 36h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to an original volume of 1/3, and thus obtaining a compound extraction concentrate;
(3) according to the mass parts, 10 parts of citric acid, 20 parts of neoagarotetraose, 10 parts of fructooligosaccharide, 5 parts of pectin and 300 parts of purified water are uniformly mixed with the compound extraction concentrated solution, are homogenized and dispersed, are sterilized at 145 ℃ for 6s, and are filled.
Example 4
A beverage for relieving body fatigue is prepared from the following raw materials in parts by weight: 6 parts of tartaric acid; 8 parts of cane sugar; 14 parts of neoagarotetraose; 7 parts of fructooligosaccharide; 13 parts of soft-shelled algae, spirulina, hermatypium, kelp, underclady algae and sargassum pallidum extract concentrated solution; 3 parts of pectin; 300 parts of purified water.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 2:7:4:12:7:6, adding 10 times of purified water, and homogenizing and pulping; taking 13 parts of an extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h, filtering the residue to obtain a slurry, adding 8 parts of sucrose, adding 2 parts of lactobacillus acidophilus microbial inoculum, fermenting for 28h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to an original volume of 1/3 to obtain a compound extract concentrate;
(3) 6 parts of tartaric acid, 14 parts of neoagarotetraose, 7 parts of fructooligosaccharide, 3 parts of pectin and 300 parts of purified water according to the mass parts are uniformly mixed with the compound extraction concentrated solution, homogenized and dispersed, sterilized for 7s at the temperature of 141 ℃, and filled.
Example 5
A beverage for relieving body fatigue is prepared from the following raw materials in parts by weight: 6 parts of glycyrrhizic acid; 7 parts of cane sugar; 18 parts of neoagarotetraose; 9 parts of fructooligosaccharide; 18 parts of soft-shelled algae, spirulina, hermatypium, kelp, underclady algae and sargassum pallidum extract concentrated solution; 2 parts of pectin; 300 parts of purified water.
The preparation method of the beverage for relieving the body fatigue comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 4:6:4:11:9:7, adding 10 times of purified water, and homogenizing and pulping; taking 18 parts of the extracted concentrated solution;
(2) stirring the homogenate at 80 ℃ for 2h, filtering the residue to obtain a slurry, adding 7 parts of sucrose, adding 1 part of bifidobacterium microbial inoculum, fermenting for 32h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to 1/3 of the original volume to obtain a compound extract concentrated solution;
(3) according to the mass parts, 6 parts of glycyrrhizic acid, 18 parts of neoagarotetraose, 9 parts of fructooligosaccharide, 2 parts of pectin and 300 parts of purified water are uniformly mixed with the compound extraction concentrated solution, homogenized and dispersed, sterilized at 139 ℃ for 9s and filled.
Animal experiments:
the following experiment was performed using the beverage for relieving body fatigue prepared in example 1.
And establishing and grouping exhaustive exercise mouse models. Balb/c mice were fed adaptively for 1 week and randomly divided into 3 groups by body weight, 8 mice per group, i.e., normal group (Control), exhaustive exercise group (Motion), and drink group (NAT). Free-feeding mice were fed with AIN-93M as a standard maintenance feed. Indoor temperature is 21-24 ℃, relative humidity is 40-55%, air circulation is realized, and illumination time is l2 h. Normal group was perfused with normal saline without exhaustive motor intervention; model group and beverage group, using mouse wheel type fatigue instrument to give 2.5h exhaustion exercise, rotating speed of 20 r/min, continuous exercise for 2 days, and interval for 5 days. The same exhaustive exercise program was executed for 16 days. The beverage group perfuses stomach 2.5g/kg of beverage while doing exhaustive exercise, and the model group perfuses stomach equivalent normal saline.
The blood sugar, blood lactic acid, liver glycogen and muscle glycogen storage, superoxide dismutase activity and creatine kinase activity of the mice in each experimental group are measured. As a result, as shown in fig. 1, the exercise of exhaustion resulted in a decrease in blood glucose level, an increase in blood lactate level, and a decrease in the storage of hepatic and muscle glycogen, causing oxidative damage to the intestinal tract, significantly decreasing the SOD level; the above results all indicate that exhaustive exercise results in a significant fatigue stress state in mice. After the beverage is infused into the stomach, the fatigue stress state is obviously recovered.
And (3) measuring indexes such as intestinal villus length, crypt depth, tight junction protein expression, endotoxin level and the like of the mice of each experimental group. The results are shown in fig. 2, and the exhaustive movement causes the structural destruction of the small intestine, which is shown in the following specific points: the small intestine villus length is reduced, and the ratio of villus length to crypt depth is significantly reduced. The beverage can obviously protect the structural integrity of the small intestine under exhaustive exercise. Meanwhile, researches show that the beverage can obviously increase the expression of intestinal tight junction protein, reduce the intestinal permeability and inhibit endotoxin from entering blood.
The content of short chain fatty acid in the feces of the mice in each experimental group is measured. The results are shown in figure 3, where the beverages of the present invention are capable of significantly increasing fecal short chain fatty acid levels, particularly acetate, propionate, and butyrate. Meanwhile, the detection shows that the abundance of the short chain fatty acid producing bacteria (SCFAs) is also increased to a certain extent. Further explanation is as follows: the beverage can increase the content of the short-chain fatty acid by proliferating the short-chain fatty acid producing bacteria. The short chain fatty acid is a product of polysaccharide metabolism by bacteria, can promote the proliferation of intestinal epithelial cells, particularly the generation of butyrate, supplies energy to the intestinal epithelial cells, repairs intestinal barriers and relieves intestinal injury caused by exhaustive exercise.
The results are shown in figure 4, α diversity and β diversity are respectively counted, the results show that the intervention of the beverage has no significant influence on α diversity index, Shannon and Simpson indexes, the Beta diversity is further counted, and the intragroup comparison and the intergroup comparison of all experimental samples are respectively carried out by adopting an Unweighted UniFrac Distance algorithm and a Weighted UniFrac Distance algorithm.
20 mice were randomly divided into 2 groups of 10 mice each, a placebo group (2.5g/kg physiological saline) and a drink group (2.5g/kg drink), and were gavaged 1 time every 1 day for 20 consecutive days. After the last administration for 1h, a lead block with the mass of 5% of the tail root of the mouse is wound on the tail root of the mouse, the mouse is placed in a constant-temperature water bath box with the water depth of 25cm and the water temperature of 25 +/-0.5 ℃, and the time from the water entry to the exhaustion of the mouse immersed in the water for 9s without floating out of the water surface is recorded as the weight-bearing movement time of the mouse. The average swimming time under load of the control group mice is 5.63 +/-0.78 min, and the average swimming time under load of the beverage group mice is 8.76 +/-0.65 min. The result shows that the beverage can effectively relieve the sports fatigue of the mice and obviously prolong the average weight bearing swimming time of the mice.
Claims (1)
1. The beverage for improving the content of short-chain fatty acid is characterized by comprising the following components in parts by weight: 8 parts of malic acid, 6 parts of sucrose, 20 parts of neoagarotetraose, 5 parts of fructooligosaccharide, 15 parts of a soft-shelled alga, spirulina, hermatypium, kelp, underclothes and sargassum pallidum extract concentrate, 3 parts of pectin, 2 parts of lactobacillus plantarum and 300 parts of purified water;
the preparation method of the beverage for improving the content of the short-chain fatty acid comprises the following steps:
(1) uniformly mixing the soft-shelled algae, spirulina, monostroma taiwanensis, kelp, undercladium, and artemisia annua according to the mass ratio of 2:5:3:10:8:6, adding 10 times of purified water, and homogenizing and pulping; taking 15 parts of an extracted concentrated solution;
(2) keeping the temperature of the extracted concentrated solution at 80 ℃ and stirring for 2h, filtering dregs to obtain a slurry, adding 6 parts of sucrose, carrying out ultrahigh-temperature instantaneous sterilization, adding 2 parts of lactobacillus plantarum to ferment for 36h, centrifuging to obtain a supernatant, evaporating and concentrating the supernatant to an original volume of 1/3, thus obtaining a compound extracted concentrated solution;
(3) 8 parts of malic acid, 20 parts of neoagarotetraose, 5 parts of fructooligosaccharide, 3 parts of pectin and 300 parts of purified water according to the mass parts are uniformly mixed with the compound extraction concentrated solution, homogenized and dispersed, sterilized at 138 ℃ for 8s and filled.
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| CN1208620A (en) * | 1997-08-15 | 1999-02-24 | 陈秀枢 | Composite contg. bacterium bifidum and lactobacillus |
| CN1411756A (en) * | 2002-11-20 | 2003-04-23 | 江明权 | Pure natural health-care beverage and its preparation method |
| CN104095897A (en) * | 2014-06-24 | 2014-10-15 | 伏思思 | Maca dunaliella salina compound preparation |
| CN106107374A (en) * | 2016-07-02 | 2016-11-16 | 威海御膳坊生物科技有限公司 | A kind of spirulina health-care beverage and preparation method thereof |
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2016
- 2016-12-14 CN CN201611149403.8A patent/CN106578765B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1208620A (en) * | 1997-08-15 | 1999-02-24 | 陈秀枢 | Composite contg. bacterium bifidum and lactobacillus |
| CN1411756A (en) * | 2002-11-20 | 2003-04-23 | 江明权 | Pure natural health-care beverage and its preparation method |
| CN104095897A (en) * | 2014-06-24 | 2014-10-15 | 伏思思 | Maca dunaliella salina compound preparation |
| CN106107374A (en) * | 2016-07-02 | 2016-11-16 | 威海御膳坊生物科技有限公司 | A kind of spirulina health-care beverage and preparation method thereof |
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