CN106578721A - Enzymatically-hydrolyzed chicken blood meal and preparation method and application thereof - Google Patents
Enzymatically-hydrolyzed chicken blood meal and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses enzymatically-hydrolyzed chicken blood meal and a preparation method and application thereof. The enzymatically-hydrolyzed chicken blood meal is prepared by mixing chicken blood with an anticoagulant, carrying out enzymatic hydrolysis I with neutral proteinase, carrying out II with acid proteinase, and drying. Experiments discover that by replacing fish meal with the enzymatically-hydrolyzed chicken blood meal, it is possible to significantly increase the growth speed and immune level of common carp and to reduce feed cost; the enzymatically-hydrolyzed chicken blood meal may be used as a high-quality protein for use in aquatic feeds.
Description
Technical field
The present invention relates to a kind of enzymolysis chicken blood meal and preparation method and application.
Background technology
Many butchering fowl factories slaughtering process produces a large amount of chicken bloods, without utilization well, causes to waste and pollutes.In chicken
In blood, protein content is up to more than 70% (in terms of dry) and Organic Iron, and there is substantial amounts of essential amino acid.In chicken blood
In liquid, hyperglobulinemia accounts for total protein 2/3, but hyperglobulinemia is in haemocyte, with the protection of one layer of cells film, makes it difficult to degraded
Absorb, pretend for feed digestibility it is low, meanwhile, in chicken blood contain potentially large number of germ, limit the use in feed.It is logical
Crossing enzymolysis can improve the content of peptide in blood meal, but traditional enzyme solution, it is easy to corrupt micro- life is polluted in enzymolysis process
Thing, causing the production of putrefactive microorganisms causes blood meal putrid and deteriorated, so as to produce VBN (VBN), affects product
Quality.
Fish meal is the major protein raw material sources of aquatic feeds, and recently as shortage of resources, the price of fish meal is year by year
Go up, cause aquatic feeds cost surging, the alternate resources for seeking high-quality are a big focuses of current aquatic feeds research.
The content of the invention
It is an object of the invention to provide a kind of enzymolysis chicken blood meal and preparation method and application.
The method of the preparation enzymolysis chicken blood meal that the present invention is provided, in whole process, the condition for being digested is not suitable for
The growth of putrefactive microorganisms, so overcoming traditional enzyme solution is easily caused rotten and VBN the increase of blood meal,
And the sour molten albumen (peptide) of high-load can not be obtained.The method comprises the steps：
After chicken blood is mixed with anti-coagulants, enzymolysis I is carried out with neutral proteinase, then enzymolysis II is carried out with acid protease,
It is dried, obtains the enzymolysis chicken blood meal.
In said method, the anti-coagulants is liquaemin, sodium oxalate or sodium citrate；
Mass percentage concentration of the anti-coagulants in the system being made up of chicken blood and anti-coagulants is 0.5-3.5%, specifically
For 1.5%.
In the enzymolysis I steps, the enzyme activity of the neutral proteinase is 5000-8000U/ml, specially 7000U/ml；
1 enzyme activity unit of the enzyme activity of the neutral proteinase is referred in 45 DEG C of optimum temperature, under the conditions of optimal pH 7.0,
The enzyme amount of 1 microgram casein protein can be converted in 1 minute.
The consumption of the neutral proteinase is every 1ml chicken bloods neutral proteinase 5-8U, concretely 5U, 6U or
7U；
Temperature is 40-55 DEG C, specially 50 DEG C；
Time be 3-7 hours, specially 5 hours；
PH value is 6-8, specially 7.
In the enzymolysis II step, the enzyme activity of the acid protease is 5000-8000U/ml, specially 6000U/ml；
Wherein, 1 enzyme activity unit of acid protease enzyme activity referred in 40 DEG C of optimum temperature, under the conditions of optimal pH 3.0,
The enzyme amount of 1 microgram casein protein can be converted in 1 minute.
The consumption of the acid protease is every 1ml chicken bloods acid protease 5-8U, concretely 5U, 6U or
7U；
Temperature is 40-45 DEG C, specially 45 DEG C；
Time be 3-5 hours, specially 3 hours；
PH value is 3-5, specially 3.
In the drying steps, drying means is spray drying；
The dry time be 20-30 minutes, specially 25 minutes；
Temperature is 180-210 DEG C, specially 210 DEG C.
In addition, preparing enzymolysis chicken blood meal and any one in following application according to the method described above, this is fallen within
The protection domain of invention：
1) application in as fish feed additive；
2) application in feeding fish.
3) application in fish growth is promoted；
4) application in the activity of fish alkaline phosphatase is improved；
5) application in fish in the activity of superoxide dismutase is improved；
6) application in catalatic activity in fish is improved；
7) application of fish immunity is improved.
In the enzymolysis chicken blood meal, the weight/mass percentage composition of crude protein is 65-67%, specially 65.3%；
The weight/mass percentage composition of the molten albumen (peptide) of acid is 35.0-52.5%, specially 45.2%.
Content Wei≤the 30mg/100g of VBN (VBN), specially 12.5mg/100g.
The fish concretely fresh-water fishes, more specifically crucian.
The consumption of the enzymolysis chicken blood meal is the 10%-70% of the enzymolysis chicken blood meal and fish meal gross weight, specially
10%-30% or 10%-50%；
The fish meal concretely fish meal.
Chicken blood hyperglobulinemia is resolved into peptide by the present invention using efficient zymolysis technique, and it is amino acid that peptide is protein degradation
Intermediate product, easily absorbed by animal.Meanwhile, blood meal produces substantial amounts of fishy smell stink during enzymolysis, and adopts
Deodorant with equipment, relatively costly, this research is cultivated using three kinds of probios, studies its deodorant except raw meat effect.Present invention choosing
The tail crucians of l 242 [just counterpoise (46.14_+0.13) g] are taken, 6 groups have been randomly divided into, per group of 3 repetitions, respectively feeding has substituted base
0,10,30,50,70 and 100% fish meal in plinth feed, determines carassius auratus growth, immunity, body index etc. after off-test
Index.As a result show：1) with control group ratio, after substituting 30% fish meal, group carassius auratus end counterpoise, rate of body weight gain and specific growth rate are equal
Control group (0% group) is significantly higher than, and substitutes 50,70% two group of fish meal with control group difference less, after substituting 100% fish meal,
Its growth and body index are less than control group.Substituting 30-70% fish meal can improve the immune indexes of crucian.It follows that replacing
The fish meal in generation 30%, can improve growth, the body index of crucian, while immune indexes are improved, while improve the non-of fish body
Specific immune function and premunition.It can be seen that, using enzymolysis chicken blood meal substitute fish meal, can significantly improve crucian the speed of growth and
Immune level, reduces feed cost, can be as high-quality protein used in aquatic feeds.
Specific embodiment
With reference to specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Institute
State method and be conventional method if no special instructions.The raw material can be obtained if no special instructions from open commercial sources.
Embodiment 1,
1. test material and method
Material
Neutral proteinase (100,000 U/ml) optimum temperature 40-50 DEG C, optimal pH=6-8 is purchased from Jinan day Scents of Heaven, Inc., (US) 550 Fannin Street, Suite 104, Beaumont, Texas 77701
Acid protease (100,000 U/ml) optimum temperature 35-50 DEG C, optimal pH=4-6 is purchased from Jinan day Scents of Heaven, Inc., (US) 550 Fannin Street, Suite 104, Beaumont, Texas 77701
Chicken blood：Purchased from Shandong Fengxiang group
1.2. test method
1.2.1 the anti-freezing of raw material is processed
This experiment carries out processing new fresh poultry blood with different anti-coagulants and variable concentrations respectively.Respectively will be different anti-
Solidifying agent is added in 200ML beakers, is separately added into different anticoagulant sodium heparins, sodium oxalate, sodium citrate, be separately added into 0.25,
0.5th, 0.75,1,1.25,1.5,1.75,2g, subsequently rapidly joins the new fresh poultry bloods of 50ml, now anti-freezing agent concentration be respectively 0.5,
1st, 1.5,2,2.5,3,3.5%, stirring stands after 0.5 minute, and according to solidification situation the anti-freezing concentration of different anti-coagulants is judged.
1.2.2 the determination of enzymatic hydrolysis condition
1.2.2.1 enzymolysis PH, enzyme activity and enzymolysis time are determined
Different protease are different to the cleavage site of albumen, meanwhile, the optimum temperature of different protease, PH are different, in order to
It is determined that most suitable enzymatic hydrolysis condition, using orthogonal test, optimization neutral proteinase and acid protease, is respectively provided with three gradients,
The consumption for making neutral proteinase in every ml blood is 5,6,7U, the consumption of acid protease is 5,6,7U, wherein, neutral protein
Enzyme temperature is 45 DEG C, and acid protease temperature is carried out for 50 DEG C, using incomplete L4(53) orthogonal test, study its chicken blood enzymolysis
Condition.The acid protease and neutral proteinase of different enzyme activity are added in blood respectively, with sodium dihydrogen phosphate and phosphoric acid hydrogen two
Sodium adjusts pH so as in 7.0,5.0,3.0 3 gradients, and temperature control is 40,45,50 DEG C of three levels.Setting level is as follows：
a
The proteolysis assay of table 1 is horizontally disposed with
| Level | pH | Temperature | Enzymatic activity (U/ml blood) | Enzymolysis time h |
| 1 | 7 | 40 | 5 | 1 |
| 2 | 5 | 45 | 6 | 3 |
| 3 | 3 | 50 | 7 | 5 |
Table 2 digests embodiment
| Tested number | PH | Temperature | Enzyme activity (U/ml blood) | Enzymolysis time h |
| 1 | 7 | 40 | 5 | 1 |
| 2 | 7 | 45 | 6 | 3 |
| 3 | 7 | 50 | 7 | 5 |
| 4 | 5 | 40 | 5 | 1 |
| 5 | 5 | 45 | 6 | 3 |
| 6 | 5 | 50 | 7 | 5 |
| 7 | 3 | 40 | 5 | 1 |
| 8 | 3 | 45 | 6 | 3 |
| 9 | 3 | 50 | 7 | 5 |
1.2.2.2 hydrolysis temperature is determined
Because the optimum temperature of acid protease is 35-50 DEG C, neutral proteinase is 40-50 DEG C, for Simplified flowsheet, is adopted
With unified temperature, by arranging different temperatures gradient, 35,40,45,50 DEG C are digested, respectively with PH, the enzyme having determined
Living, enzymolysis time is optimized.
1.2.2.2 the determination of enzymolysis order
Respectively in the chicken blood for adding anti-coagulants, sodium dihydrogen phosphate is added, adjusts PH=5, carry out acid protease enzymolysis,
Then adjusting PH=7 with disodium hydrogen phosphate carries out neutral protease enzymolysis, and neutral protease enzymolysis is carried out in first, after carry out acid
Property protease hydrolyzed compare, compare peptide content.
1.2.2.4 the research of deodorizing effect
Respectively after enzymolysis, 10 are added in enzymatic vessel5CFU saccharomyces cerevisiaes, 105CFU lactic acid bacterias and 105CFU bacillus subtilis
Bacterium, stands to place respectively and places 0,0.5,1,1.5 hours with 10RPM stirrings, determines enzymatic vessel NH3Content.
1.2.3 the determination of drying condition
Spray drying process, oven drying method, expansion drying, vacuum drying is respectively adopted, compare this several method the rate of recovery and
Drying time (moisture terminates for 10% for drying), the optimum drying means of screening.The small-sized spray drier of 10kw is selected in test,
The drying baker of 8kw, the continuous vacuum dryer of 12kw and the small-sized flash dryer of 10kw are compared.
1.2.4 testing index and method
The measure of the molten albumen (peptide) of acid：Determined using trichloroacetic acid method, carried out with reference to GB/T22492-2008 methods；
The measure of crude protein：The crude protein of enzymolysis chicken blood meal and blood meal adopts Kjeldahl nitrogen determination, with reference to GB9005.5-
2010 methods are carried out；
Molten albumen (peptide) ratio=sour molten albumen/crude protein of measure acid of the molten Protein ratios of acid
Ammonia is determined：Using pernicious gas survey meter, NH is determined3Content is so that it is determined that deodorizing effect.
2. result of the test and discussion
2.1 different anti-coagulants process chicken blood effect
The anti-chicken blood effect of the different anti-coagulants of table 3
- represent not with anticoagulation ,+represent anticoagulation ++ represent that there is stronger anticoagulation
Found by upper table, different anti-coagulants are different to the anti-freezing concentration of chicken blood, wherein, the effect of liquaemin is best,
The effect of anti-freezing is can reach under conditions of 1%, under conditions of 1.5%, anticoagulant effect is stronger.And sodium oxalate and sodium citrate
Anticoagulant effect is a little compared with heparin sodium error.Therefore the liquaemin of selection 1.5% is used as anti-coagulants.
The determination of 2.2 enzymatic hydrolysis conditions
The protease hydrolytic parametric results of table 4
Found by result of the test, neutral proteinase 7U/ml blood, in PH=7,50 DEG C are processed 5 hours, peptide in enzymolysis liquid
Content is up to 34.5%, and acid protease 6U/ml blood, in pH=3, temperature is 45 DEG C and processes 3 hours, peptide in enzymolysis liquid
Content is up to 30.9%.
It is found through experiments, generation of the enzymolysis order on peptide affects less, first neutral proteinase after acidity, first acid after neutrality
Property protease, after enzymolysis be respectively 45.6% and 45.8%, difference is little.
2.3 microbial deodorant effects compare
During proteolysis, because blood itself fishy smell and protein degradation are into peptide and amino acid, produce big
Amount bad smell gas, main component is ammonia, the element evaporation mixture of the various ammonia nitrogens of fishy smell.Here different probios are compared
Removal effect to these gases in later stage enzymolysis liquid.Test finds, using the saccharomycete of 105CFU/ml, the NH in enzymatic vessel3
Gas is minimum, is 12.2ppm, and lactic acid bacteria change is little, and bacillus subtilis has rising NH3Effect, so select
S. cervisiae is most suitable biological deodorizing bacterial strain, and the deodorant time is 1 hour.
Ammonia concentration (ppm) after the different time of table 5 and bacterial strain process in enzymatic vessel
The determination of 2.4 drying modes
The rate of recovery of 2.4 different drying modes and it is dried 1kg/ sample times and compares
The rate of recovery of the different drying modes of table 6 and it is dried 1kg/ sample times and compares
| Drying mode | Rate of recovery % | Drying time min |
| It is spray-dried | 90.2 | 25 |
| Drying drying | 87.5 | 120 |
| Expansion drying | 80.5 | 30 |
| Vacuum drying | 91.5 | 120 |
Test finds, using oven for drying and vacuum drying, required time all about 120 minutes, the time considerably longer than sprays
It is dried and expansion drying, meanwhile, the rate of recovery of spray drying is slightly above expansion drying, so select to be spray-dried being dried for optimum
Mode, temperature is 210 DEG C.
Test by more than, determine that technology path mixes for chicken blood with anti-coagulants, final concentration of 1.5% is allowed to, by phosphorus
Sour disodium hydrogen and sodium dihydrogen phosphate regulation pH, neutral proteinase 7U/ml blood, in PH=7,50 DEG C are processed 5 hours, acidic protein
In pH=3, temperature is 45 DEG C and processes 3 hours enzyme 6U/ml blood, is spray-dried and obtains enzymolysis chicken blood meal.Through determining now enzyme
Solution chicken blood meal, crude protein is 65.3%, and sour molten albumen (peptide) is 45.2%, and sour molten protein ratio is 45.2/65.3=69.2, i.e.,
69.2 albumen is degraded to peptide, and VBN is 13.5mg/100g (GB/T5009.44-2003).
The application of embodiment 2, enzymolysis chicken blood meal
1 materials and methods
1.1 test feed
Crucian basal feed is constituted and Major Nutrient level is shown in Table 6.The size-reduced mesh sieve of mistake 40 of feedstuff, is well mixed
Afterwards, 2mm particles are processed into standby.Test is divided into 6 groups, and feed group based on the 1st group, other 2-6 groups are different enzymolysis chicken bloods
Powder substitutes fish meal ratio.Basal feed is according to China《Crucian feeding standard》(2004) trophic level recommended is prepared.
Embodiment enzymolysis chicken blood meal used is obtained according to the gained optimum process of embodiment 1, namely：
Chicken blood is mixed with anticoagulant sodium heparin, quality percentage of the liquaemin in the system being made up of chicken blood and liquaemin
Concentration is 1.5%, pH is adjusted by disodium hydrogen phosphate and sodium dihydrogen phosphate, with the neutral proteinase that enzyme activity is 7000U/ml in PH
Enzymolysis processing 5 hours under the conditions of=7,50 DEG C, the consumption of neutral proteinase is every 1ml chicken bloods neutral proteinase 7U, then is used
The acid protease of 6000U/ml is enzymolysis processing 3 hours under the conditions of 45 DEG C in pH=3, temperature, and the consumption of acid protease is
Per 1ml chicken bloods acid protease 6U, then it is spray-dried 25 minutes at 210 DEG C, obtains enzymolysis chicken blood meal.
1.2 feeding and management
Test fish is tamed and dociled food and is adapted to after environment l0d, choose physique healthy and strong, neat specification from the carassius auratus fish cultivated then
Fancy carp [average weight be (10.30 ± 0.01) g] 560 tails, be randomly divided into 4 groups, 4 repetitions are set per group, each repeats 35 tails
Fish.Every group of test fish feeds at random a kind of test feed, raises in fresh water circulation glass aquarium (effective volume is 300L) indoors
Support 8 weeks, feed day rate be body weight 4%-6%, daily 08：30、12：30 and 17：30 respectively feed 1 time.Cultivation water source is aeration
Running water, timing during test is monitored to water quality, and the whole water temperature of cultivation is (27.6 ± 2.2) DEG C, and pH is 7.2 ± 0.4, molten
Solution oxygen>6.3mg/L, ammonia nitrogen<0.48m/L, nitrite nitrogen<0.06m/L.
1.3 sample collections and preparation
After feeding experiment terminates, weigh and count after fasting 24h.5 tail fishes are taken at random per cylinder and takes blood in tail vein, by blood
Sample puts 4 DEG C of refrigerator overnights, and 10min is centrifuged with 6 000r/min under the conditions of 4 DEG C, collects serum, and 20 DEG C save backup.
1.4 index determining
Serum lysozyme, alkaline phosphatase (AKP), catalase (CAT), total number born (T-SOD) are living
Property using Nanjing build up Bioengineering Research Institute production kit be measured.
Wherein, catalatic measure：
Test apparatus：Mortar, volumetric flask, test tube, micropipettor, spectrophotometer
Test procedure：Enzyme liquid is extracted：Weigh tissue 0.5g to put in mortar, add the pH7.0 phosphoric acid of precooling at 2～3ml4 DEG C
Buffer solution and a small amount of quartzite sand grind in proceeding to 25ml volumetric flasks, and, merge punching with wash buffer mortar for several times into after homogenate
Washing lotion, and constant volume is to scale.It is well mixed measuring bottle to be put in 5 DEG C of refrigerators and stands 10min, takes top clarified solution under 4000rpm
Centrifugation 15min, supernatant is hydrogen peroxide enzyme extract.Save backup at 5 DEG C.
Determine：3,10ml test tubes are taken, wherein 2 is sample determination pipe, 1 is blank tube, and by table order reagent is added.
After 25 DEG C of preheatings, by pipe the H of 0.3ml0.1mol/L is added2O2, often add a pipe and clock immediately, and pour quartz cuvette into rapidly
In, mensuration absorbance under 240nm, every 1min readings 1 time, surveys altogether 4min, after 3 arms have all been determined, is calculated as follows
Enzyme activity.
The ultraviolet absorption method of table 7 determines H2O2Sample liquid allocation list
| Guan Hao | S0 | S1 | S2 |
| Crude enzyme liquid (ml) | 0.2 (boiling dead enzyme liquid) | 0.2 | 0.2 |
| PH7.8 phosphate buffers (ml) | 1.5 | 1.5 | 1.5 |
| Distilled water (ml) | 1.0 | 1.0 | 1.0 |
Catalase enzyme activity (U)=Δ A240×VT/0.1×V1×t×FW
ΔA240=AS0-(AS1-AS2)/2
AS0- add the control tube light absorption value for boiling dead enzyme liquid；
AS1,AS2- sample cell light absorption value；
Vt-thick zyme extract cumulative volume (ml)；
V1-measure crude enzyme liquid volume (ml)；
FW-sample fresh weight (g)；
0.1-A240 often declines 0.1 for 1 enzyme-activity unit (u)；
T-add hydrogen peroxide to last time reading duration (min).
Alkaline phosphatase (AKP) is determined
Test apparatus：Mortar, volumetric flask, test tube, micropipettor, spectrophotometer, water-bath, centrifuge
Test procedure：
(1) acquired blood 8000rpm is centrifuged 10 minutes, Aspirate supernatant is serum；
(2) related solution is added according to table 8 below：
Table 8 configures solution
| Blank tube | Standard pipe | Determine pipe | |
| Distilled water | 0.05 | ||
| 0.1mg/ml phenol standard reagents | 0.05 | ||
| Serum | 0.05 | ||
| Phosphate buffer 1 | 0.5 | 0.5 | 0.5 |
| Matrix liquid | 0.5 | 0.5 | 0.5 |
After above test tube is well mixed, 37 degree of water-baths 15 minutes add nitrite ion, determine absorbance under each pipe 520nm.Meter
Calculate formula as follows：AKP=determines pipe OD/ standard pipes OD × 0.05 × 100ml/0.05ml
The measure of superoxide dismutase SOD：
Test apparatus：Mortar, volumetric flask, test tube, micropipettor, spectrophotometer
Test procedure：(1) will take tissue 0.1g adds phosphate buffer 1ml to grind, and 5000rpm is centrifuged 3 minutes,
Supernatant is taken for sample
(2) related solution is added according to table 9 below
Table 9 configures solution
| Reagent ml | Determine pipe | Control tube |
| Reagent one | 1.0 | 1.0 |
| Sample | a | |
| Distilled water | a | |
| Reagent two | 0.1 | 0.1 |
| Reagent three | 0.1 | 0.1 |
| Reagent four | 0.1 | 0.1 |
After above test tube is well mixed, 37 degree of water-baths 40 minutes add nitrite ion, determine absorbance under each pipe 550nm.Meter
Calculate formula as follows：SOD=(control tube OD- determines pipe OD)/control tube OD/50% × reactant liquor cumulative volume/sampling amount a/ is to be measured
Example weight (0.1g)
Specific growth rate=100 × [ln ends weight (g)-ln initial weight (g)]/test number of days (d)；
Feed coefficient=total solids food ration (g)/fish body total augment weight (g)；
Survival rate=100 × end end fish tail number (tail)/initial fish tail number (tail).
1.7 data process＆analysis
Data represent with mean value and standard error, and one-way analysis of variance (one- is carried out to the data obtained using SPSS
WayANOVA), if difference reaches significance, significance is P<0.05.
The nutrient content of the different disposal group of table 10
2 results and analysis
Impact of the chicken blood meal to crucian growth performance is digested in 2.1 feeds
As can be seen from Table 11, with the increase of substitution ratio, the last heavy, growth rate of crucian and feed coefficient reach maximum
(substitute 50% fish meal) afterwards, as substitution ratio further increases, growth performance is reduced on the contrary.Find simultaneously, difference is substituted
Ratio does not result in crucian death, therefore it was determined that substitutes 50% fish meal, growth performance is optimal.
Table 11 digests impact of the chicken blood meal to crucian growth performance
Colleague's data shoulder note different lowercase letter indication differences significantly (P<0.05).
Impact of the 2.2 enzymolysis chicken blood meals to crucian nonspecific immunity
Table 12 digests impact of the chicken blood meal to crucian nonspecific immunity
Colleague's data shoulder note different lowercase letter indication differences significantly (P<0.05).
Test finds that enzymolysis chicken blood meal can significantly improve crucian alkaline phosphatase, superoxide dismutase and hydrogen peroxide
The activity of enzyme, and lysozyme is not affected.
Impact to alkaline phosphatase：Enzymolysis chicken blood meal substitutes 30% fish meal and can significantly improve the work of alkaline phosphatase
Property, but with the increase of substitution ratio, alkaline phosphatase is not affected.
Impact to superoxide dismutase：Enzymolysis chicken blood meal substitutes 10% fish meal section and significantly improves superoxide dismutase
The activity of enzyme, but will not improve with the increase of substitution ratio.
On catalatic impact：Enzymolysis chicken blood meal substitutes 10% fish meal section and significantly improves superoxide dismutase
Activity, but will not improve with the increase of substitution ratio.
In a word, digesting the fish meal of chicken blood meal replacement 30% can improve the growth for promoting crucian, reduce feed coefficient, improve alkali
Acid phosphatase and superoxide dismutase and catalase, illustrate that the peptide in its enzymolysis chicken blood meal has and promote that animal is congenital exempts from
The effect of epidemic disease.
Claims (10)
1. a kind of method for preparing enzymolysis chicken blood meal, comprises the steps：
After chicken blood is mixed with anti-coagulants, enzymolysis I is carried out with neutral proteinase, then enzymolysis II is carried out with acid protease, be dried,
Obtain the enzymolysis chicken blood meal.
2. method according to claim 1, it is characterised in that：The anti-coagulants is liquaemin, sodium oxalate or sodium citrate；
Mass percentage concentration of the anti-coagulants in the system being made up of chicken blood and anti-coagulants is 0.5-3.5%, specially
1.5%.
3. method according to claim 1 and 2, it is characterised in that：The enzyme activity of the neutral proteinase is 100000-
150000U/ml, specially 10000U/ml；
The consumption of the neutral proteinase is every 1ml chicken bloods neutral proteinase 5-8U；
Temperature is 40-55 DEG C, specially 50 DEG C；
Time be 3-7 hours, specially 5 hours；
PH value is 6-8, specially 7.
4. according to arbitrary described method in claim 1-3, it is characterised in that：In the enzymolysis II step, the acid egg
The enzyme activity of white enzyme is 100000-150000U/ml, specially 10000U/ml；
The consumption of the acid protease is every 1ml chicken bloods acid protease 5-8U；
Temperature is 40-45 DEG C, specially 45 DEG C；
Time be 3-5 hours, specially 3 hours；
PH value is 3-5, specially 3.
5. according to arbitrary described method in claim 1-4, it is characterised in that：In the drying steps, drying means is spray
Mist is dried；
The dry time be 20-30 minutes, specially 25 minutes；
Temperature is 180-210 DEG C, specially 210 DEG C.
6. the enzymolysis chicken blood meal that any one of claim 1-5 is prepared.
7. chicken blood meal is digested according to claim 6, it is characterised in that：In the enzymolysis chicken blood meal, the quality hundred of crude protein
Divide content for 65-67%, specially 65.3%；
The weight/mass percentage composition of the molten albumen of acid is 35.0-52.5%, specially 45.2%；
Content Wei of VBN≤30mg/100g, specially 12.5mg/100g.
8. any one of chicken blood meal in following application is digested described in claim 6 or 7：
1) application in as fish feed additive；
2) application in feeding fish.
3) application in fish growth is promoted；
4) application in the activity of fish alkaline phosphatase is improved；
5) application in fish in the activity of superoxide dismutase is improved；
6) application in catalatic activity in fish is improved；
7) application of fish immunity is improved.
9. application according to claim 8, it is characterised in that：The fish is fresh-water fishes, specially crucian.
10. application according to claim 8 or claim 9, it is characterised in that：The consumption of the enzymolysis chicken blood meal is the enzymolysis chicken
The 10%-70%, specially 10%-30% or 10%-50% of blood meal and fish meal gross weight；
The fish meal is fish meal.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108271951A (en) * | 2018-02-10 | 2018-07-13 | 中国科学院水生生物研究所 | The algae source property complex protein mixtures of fish meal and application in a kind of replacement aquatic feeds |
| CN112386254A (en) * | 2020-12-09 | 2021-02-23 | 湖南源品细胞生物科技有限公司 | Collecting bag and method for preventing peripheral blood coagulation in NK cell culture |
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| CN112386254A (en) * | 2020-12-09 | 2021-02-23 | 湖南源品细胞生物科技有限公司 | Collecting bag and method for preventing peripheral blood coagulation in NK cell culture |
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