CN106578390A - Aspergillus flavus inhibitor and preparation method thereof - Google Patents
Aspergillus flavus inhibitor and preparation method thereof Download PDFInfo
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- CN106578390A CN106578390A CN201611242422.5A CN201611242422A CN106578390A CN 106578390 A CN106578390 A CN 106578390A CN 201611242422 A CN201611242422 A CN 201611242422A CN 106578390 A CN106578390 A CN 106578390A
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Abstract
The invention relates to an aspergillus flavus inhibitor and a preparation method thereof. The aspergillus flavus inhibitor is prepared from components in parts by mass as follows: 20-25 parts of herba artemisiae annuae, 20-25 parts of herba patriniae, 20-25 parts of radix arnebiae, 18-25 parts of saxifraga stolonifera, 17-21 parts of flos lonicerae, 6-10 parts of cortex fraxini, 15-20 parts of photinia leaf, 15-20 parts of common bombax flower, 1-2 parts of turmeric, 9-12 parts of radix paeoniae alba, 7-10 parts of radix bupleuri, 10-15 parts of folium mori (decocted into juice), 3-5 parts of radix glycyrrhizae, 10-15 parts of herba taraxaci, 8-12 parts of pseudobulbus cremastrae seu pleiones, 2-3 parts of rhizoma curculiginis, 2-3 parts of pepper, 5-7 parts of folium artemisiae argyi and 6-9 parts of hawthorn leaf. The aspergillus flavus inhibitor has the advantage that aspergillus flavus can be inhibited effectively.
Description
Technical field
The present invention relates to a kind of Aspergillus flavus inhibitor, further relates to a kind of preparation method of above-mentioned Aspergillus flavus inhibitor.
Background technology
Aspergillus flavus, Fungi Imperfecti, a kind of common saprophytic fungus.It is more common in mouldy grain, grain product and other mould corruption
On Organic substance.Aspergillus flavus colony growth is very fast, and short texture, surface celadon, the back side is colourless or slightly brownish.Thalline has many
Complicated branch mycelia is constituted.Vegetative hyphae has separation；A part for aerial hyphae forms long and coarse conidiophore,
Top produces flask shape or subsphaeroidal top capsule, and surface produces many stigmas (generally double-deck), on stigma generate the surface of string
Coarse spherical conidium.Conidiophore, top capsule, stigma and conidium synthesis spore head, can be used for produce amylase,
Protease and phosphodiesterase etc., are also the common strain in brewery industry.
It is 1 class carcinogen that aflatoxin in 1993 delimited by the Agency for Research on Cancer of World Health Organization (WHO) (WHO), is one
Plant the extremely strong extremely toxic substance of toxicity.The hazardness of aflatoxin is have destruction to people and animal liverss tissue, seriously
When, hepatocarcinoma can be caused even dead., mainly to the injury of animal liverss, the individuality being hurt is because of animal kind for aflatoxicosiss
Class, age, sex and nutritional statuses and it is different.Result of study shows that aflatoxin can cause liver function to decline, and reduces milk and produces
Amount and laying rate, and the immunity of animal is reduced, easily infected by harmful microbe.Additionally, long-term consumption is yellow containing low concentration
The feedstuff of aspertoxin may also lead to poisoning in embryo.Generally young animal is more sensitive to aflatoxin.Aflatoxin
Clinical manifestation be that digestive system function is disorderly, reduce fertility, reduce efficiency of feed utilization, anemia etc..Aflatoxin is not
The milk yield that milch cow can only be made declines, but also makes in milk containing the aflatoxin m1 and m2 of transition.According to american agriculture
Economist counts, the feedstuff due to eating aflatoxin contamination, at least to make U.S.'s animal husbandry be subjected to 10% Jing every year
Ji loss.In China, Aspergillus flavus equally bring serious loss to animal husbandry.Therefore, a kind of Aspergillus flavus inhibitor tool is developed
There is important meaning.
The content of the invention
An object of the present invention is to provide a kind of Aspergillus flavus inhibitor, can effectively suppress Aspergillus flavus.
In order to solve above-mentioned technical problem, the present invention is employed the following technical solutions, a kind of Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：Herba Artemisiae Annuae 20-25 parts, Herba Patriniae 20-25 parts, Radix Arnebiae (Radix Lithospermi) 20-25 parts, Herba Saxifragae 18-25 parts,
Flos Lonicerae 17-21 parts, Cortex Fraxini 6-10 parts, Folium Photiniae (Folium Photiniae serrulatae) 15-20 parts, Flos Bombacis Malabarici 15-20 parts, Rhizoma Curcumae Longae 1-2 parts, Radix Paeoniae Alba 9-12 parts, bavin
Hu 7-10 parts, Folium Mori (being boiled into juice) 10-15 parts, Radix Glycyrrhizae 3-5 parts, Herba Taraxaci 10-15 parts, Pseudobulbus Cremastrae Seu Pleioness 8-12 parts, Rhizoma Curculiginises 2-3 parts,
Fructus Piperiss 2-3 parts, Folium Artemisiae Argyi 5-7 parts, Folium Crataegi 6-9 parts.
Each raw material components of the present invention are green natural raw material, little to animal and people's toxic and side effects, can effectively suppress yellow
Aspergillosiss, and it is not likely to produce Drug resistance.With aforementioned proportion, the inhibitor as obtained by the preparation method of the present invention can be well
The bactericidal and bacteriostatic effect of each component is played, there is significant inhibition to Aspergillus flavus, being added in food or feedstuff can be with
The harm of Aspergillus flavus is reduced, is conducive to the health of human body or cultivated animals.Add the present invention's in the feedstuff of cultivated animals
Inhibitor, it is possible to decrease the impact of Aspergillus flavus, improves cultivation income.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：20 parts of Herba Artemisiae Annuae, lose
20 parts of beans grass, 20 parts of Radix Arnebiae (Radix Lithospermi), 20 parts of Herba Saxifragae, 20 parts of Flos Lonicerae, 9 parts of Cortex Fraxini, 20 parts of Folium Photiniae (Folium Photiniae serrulatae), 20 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 2
Part, 10 parts of the Radix Paeoniae Alba, 10 parts of Radix Bupleuri, 15 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, 15 parts of Herba Taraxaci, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss,
6 parts of Folium Artemisiae Argyi, 8 parts of Folium Crataegi.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：21 parts of Herba Artemisiae Annuae, lose
21 parts of beans grass, 21 parts of Radix Arnebiae (Radix Lithospermi), 21 parts of Herba Saxifragae, 21 parts of Flos Lonicerae, 6 parts of Cortex Fraxini, 19 parts of Folium Photiniae (Folium Photiniae serrulatae), 19 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 1
Part, 11 parts of the Radix Paeoniae Alba, 9 parts of Radix Bupleuri, 14 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, 14 parts of Herba Taraxaci, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss, Chinese mugwort
7 parts of leaf, 9 parts of Folium Crataegi.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：22 parts of Herba Artemisiae Annuae, lose
22 parts of beans grass, 22 parts of Radix Arnebiae (Radix Lithospermi), 22 parts of Herba Saxifragae, 18 parts of Flos Lonicerae, 7 parts of Cortex Fraxini, 18 parts of Folium Photiniae (Folium Photiniae serrulatae), 18 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 2
Part, 10 parts of the Radix Paeoniae Alba, 9 parts of Radix Bupleuri, 13 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, 13 parts of Herba Taraxaci, 10 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, Chinese mugwort
5 parts of leaf, 7 parts of Folium Crataegi.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：23 parts of Herba Artemisiae Annuae, lose
23 parts of beans grass, 23 parts of Radix Arnebiae (Radix Lithospermi), 25 parts of Herba Saxifragae, 17 parts of Flos Lonicerae, 7 parts of Cortex Fraxini, 17 parts of Folium Photiniae (Folium Photiniae serrulatae), 17 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 1
Part, 12 parts of the Radix Paeoniae Alba, 8 parts of Radix Bupleuri, 12 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, 12 parts of Herba Taraxaci, 8 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, Chinese mugwort
6 parts of leaf, 8 parts of Folium Crataegi.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：24 parts of Herba Artemisiae Annuae, lose
24 parts of beans grass, 24 parts of Radix Arnebiae (Radix Lithospermi), 18 parts of Herba Saxifragae, 21 parts of Flos Lonicerae, 10 parts of Cortex Fraxini, 16 parts of Folium Photiniae (Folium Photiniae serrulatae), 16 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 2
Part, 9 parts of the Radix Paeoniae Alba, 7 parts of Radix Bupleuri, 11 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, 11 parts of Herba Taraxaci, 9 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, Folium Artemisiae Argyi
5 parts, 9 parts of Folium Crataegi.
Preferably, the Aspergillus flavus inhibitor, is prepared from by each component of following masses number：25 parts of Herba Artemisiae Annuae, lose
25 parts of beans grass, 25 parts of Radix Arnebiae (Radix Lithospermi), 23 parts of Herba Saxifragae, 19 parts of Flos Lonicerae, 8 parts of Cortex Fraxini, 15 parts of Folium Photiniae (Folium Photiniae serrulatae), 15 parts of Flos Bombacis Malabarici, Rhizoma Curcumae Longae 1
Part, 9 parts of the Radix Paeoniae Alba, 7 parts of Radix Bupleuri, 10 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, 10 parts of Herba Taraxaci, 11 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, Chinese mugwort
7 parts of leaf, 6 parts of Folium Crataegi.
The invention also discloses a kind of above-mentioned Aspergillus flavus inhibitor is suppressing yellow as the feed additive of cultivated animals
Application during aspergillosiss, its usage and dosage is：Add in per 1kg feedstuffs 2-10g it is of the invention obtained by Aspergillus flavus inhibitor.
The invention also discloses a kind of preparation method of above-mentioned Aspergillus flavus inhibitor, it is comprised the following steps：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, filters to take the filter of gained
Liquid, and clear water of the weight for 8 times of amounts of filtering residue is added in filtering residue, it is heated to being cooked by slow fire 20-30 minutes again after boiling, filter
The filtrate of gained is taken, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water, ultrasonic wave concussion to process 20-25 minutes after it；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
After middle room temperature concussion 12h, soak under 50-60 DEG C of water bath condition under 2 hours, then 70-80 DEG C of water bath condition and soak 20-30 minutes,
Filter to take filtrate；Appropriate distilled water is added in filtering residue, is soaked 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate,
Merge the filtrate of gained twice, obtain soak；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
In preparation method, the effect that each raw material components grind is advantageous for into each raw material components in subsequent step more
Easily it is leached or extracts, is conducive to fully being reacted between the effective ingredient in each raw material components.To Herba Artemisiae Annuae, Herba Patriniae,
Add water in Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori and the soak at room temperature effect of 48 hours is to make medical material soften, be conducive to medicine
Material fully is decocted out in subsequent step.Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are put into into ultra cold storage freezer 12 little
When, it is to make cell become fragile and medical material is fully mixed to process the effect of 20-25 minutes with ultrasonic wave concussion after taking-up, is conducive to
The effective ingredient of Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri is fully leached in subsequent step, is conducive to the performance of drug effect.
Add in the mixture obtained by step (3) and the effect that room temperature in agitator shakes 12h is put into after Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness
It is the mixture obtained by step (3) is fully contacted with Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness；The work for extracting repeatedly at different temperatures
With being to make the effective ingredient in above-mentioned each component be sufficiently soaked out, and fully react.The operation purpose of step (5) be for
The effective ingredient in Folium Artemisiae Argyi, Folium Crataegi is fully extracted, and it is fully combined with the decoction liquor obtained by step (2) and anti-
Should.Room temperature shake in agitator is put after soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed
The effect for swinging 12h is the soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) is sufficiently mixed and is filled
Divide haptoreaction, the Aspergillus flavus inhibitor obtained by the present invention can be made preferably to play the inhibitory action to Aspergillus flavus.
Specifically the present invention can produce compared with prior art following good effect：
(1) each raw material components of the invention are green natural raw material, little to animal and people's toxic and side effects, can effectively suppress
Aspergillus flavus, and it is not likely to produce Drug resistance.With aforementioned proportion, the inhibitor as obtained by the preparation method of the present invention can be very well
Performance each component bactericidal and bacteriostatic effect, there is significant inhibition to Aspergillus flavus, being added to can in food or feedstuff
To reduce the harm of Aspergillus flavus, be conducive to the health of human body or cultivated animals.Add the present invention in the feedstuff of cultivated animals
Inhibitor, it is possible to decrease the impact of Aspergillus flavus, improve cultivation income.
(2) in preparation method, the effect that each raw material components grind is advantageous for into each raw material components in subsequent step
In be easier to be leached or extract, be conducive to fully being reacted between the effective ingredient in each raw material components.To Herba Artemisiae Annuae, Herba Patriniae
Add water in grass, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori and the soak at room temperature effect of 48 hours is to make medical material soften, favorably
Fully decocted out in subsequent step in medical material.Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are put into into ultra cold storage freezer
12 hours, it was to make cell become fragile and medical material is fully mixed to process the effect of 20-25 minutes with ultrasonic wave concussion after taking-up, is had
The effective ingredient of Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri is fully leached beneficial in subsequent step, is conducive to sending out for drug effect
Wave.Add in the mixture obtained by step (3) and the work that room temperature in agitator shakes 12h is put into after Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness
With being the mixture obtained by step (3) is fully contacted with Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness；Extract repeatedly at different temperatures
Effect is to make the effective ingredient in above-mentioned each component be sufficiently soaked out, and is fully reacted.The operation purpose of step (5) is
For the effective ingredient in fully extracting Folium Artemisiae Argyi, Folium Crataegi, and it is set fully to be combined with the decoction liquor obtained by step (2) and anti-
Should.Room temperature shake in agitator is put after soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed
The effect for swinging 12h is the soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) is sufficiently mixed and is filled
Divide haptoreaction, the Aspergillus flavus inhibitor obtained by the present invention can be made preferably to play the inhibitory action to Aspergillus flavus.
Specific embodiment
Below example is, for the ease of more fully understanding the present invention, but to be not intended to limit the present invention.Following enforcements
Experimental technique in example, if no special instructions, is conventional method.
Embodiment 1
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：20 parts of Herba Artemisiae Annuae, Herba Patriniae 20
Part, 20 parts of Radix Arnebiae (Radix Lithospermi), 20 parts of Herba Saxifragae, 20 parts of Flos Lonicerae, 9 parts of Cortex Fraxini, 20 parts of Folium Photiniae (Folium Photiniae serrulatae), 20 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, the Radix Paeoniae Alba
10 parts, 10 parts of Radix Bupleuri, 15 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, 15 parts of Herba Taraxaci, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss, 6 parts of Folium Artemisiae Argyi,
8 parts of Folium Crataegi.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Embodiment 2
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：21 parts of Herba Artemisiae Annuae, Herba Patriniae 21
Part, 21 parts of Radix Arnebiae (Radix Lithospermi), 21 parts of Herba Saxifragae, 21 parts of Flos Lonicerae, 6 parts of Cortex Fraxini, 19 parts of Folium Photiniae (Folium Photiniae serrulatae), 19 parts of Flos Bombacis Malabarici, 1 part of Rhizoma Curcumae Longae, the Radix Paeoniae Alba
11 parts, 9 parts of Radix Bupleuri, 14 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, 14 parts of Herba Taraxaci, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss, 7 parts of Folium Artemisiae Argyi,
9 parts of Folium Crataegi.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Embodiment 3
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：22 parts of Herba Artemisiae Annuae, Herba Patriniae 22
Part, 22 parts of Radix Arnebiae (Radix Lithospermi), 22 parts of Herba Saxifragae, 18 parts of Flos Lonicerae, 7 parts of Cortex Fraxini, 18 parts of Folium Photiniae (Folium Photiniae serrulatae), 18 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, the Radix Paeoniae Alba
10 parts, 9 parts of Radix Bupleuri, 13 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, 13 parts of Herba Taraxaci, 10 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 5 parts of Folium Artemisiae Argyi,
7 parts of Folium Crataegi.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Embodiment 4
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：23 parts of Herba Artemisiae Annuae, Herba Patriniae 23
Part, 23 parts of Radix Arnebiae (Radix Lithospermi), 25 parts of Herba Saxifragae, 17 parts of Flos Lonicerae, 7 parts of Cortex Fraxini, 17 parts of Folium Photiniae (Folium Photiniae serrulatae), 17 parts of Flos Bombacis Malabarici, 1 part of Rhizoma Curcumae Longae, the Radix Paeoniae Alba
12 parts, 8 parts of Radix Bupleuri, 12 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, 12 parts of Herba Taraxaci, 8 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 6 parts of Folium Artemisiae Argyi, mountain
8 parts of short, bristly hair or beard leaf.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Embodiment 5
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：24 parts of Herba Artemisiae Annuae, Herba Patriniae 24
Part, 24 parts of Radix Arnebiae (Radix Lithospermi), 18 parts of Herba Saxifragae, 21 parts of Flos Lonicerae, 10 parts of Cortex Fraxini, 16 parts of Folium Photiniae (Folium Photiniae serrulatae), 16 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, the Radix Paeoniae Alba
9 parts, 7 parts of Radix Bupleuri, 11 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, 11 parts of Herba Taraxaci, 9 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 5 parts of Folium Artemisiae Argyi, mountain
9 parts of short, bristly hair or beard leaf.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Embodiment 6
A kind of Aspergillus flavus inhibitor, is prepared from by each component of following masses number：25 parts of Herba Artemisiae Annuae, Herba Patriniae 25
Part, 25 parts of Radix Arnebiae (Radix Lithospermi), 23 parts of Herba Saxifragae, 19 parts of Flos Lonicerae, 8 parts of Cortex Fraxini, 15 parts of Folium Photiniae (Folium Photiniae serrulatae), 15 parts of Flos Bombacis Malabarici, 1 part of Rhizoma Curcumae Longae, the Radix Paeoniae Alba 9
Part, 7 parts of Radix Bupleuri, 10 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, 10 parts of Herba Taraxaci, 11 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 7 parts of Folium Artemisiae Argyi, mountain
6 parts of short, bristly hair or beard leaf.
A kind of preparation method of above-mentioned Aspergillus flavus inhibitor, its preparation method is as follows：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times
The clear water of weight, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, every 5- during decoction
Once, the filtrate of gained is filtered to take in decoction after finishing, and weight is added in filtering residue for the clear of 8 times of amounts of filtering residue for stirring in 10 minutes
Water, is heated to being cooked by slow fire 20-30 minutes again after boiling, stirs once every 5-10 minutes during decoction, decocts mistake after finishing
Filtrate obtained by leaching, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken
Go out, recover to room temperature to add appropriate distilled water after it, to not having medical material completely, ultrasonic wave concussion processes 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator
In with the rotating speed room temperature of 100-150r/min concussion 12h after, soak 2 hours under 50-60 DEG C of water bath condition, then 70-80 DEG C of water-bath
Under the conditions of soak 20-30 minutes, filter to take filtrate；Appropriate distilled water is added in filtering residue, the weight of the distilled water of addition is filter
5 times of slag weight, soak 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merge the filtrate of gained twice, must soak
Bubble liquid；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, the extraction being positioned in supercritical extraction instrument
In kettle, utilize carbon dioxide as medium and extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts and shake
Swing in device and 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the thick paste that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Shape fluid extract, bottling.
Bacteriostatic experiment
Culture medium：Czapek's medium, heating for dissolving, 121 DEG C of sterilizing 15-20min after subpackage.
Strain is：Aspergillus flavus (ATCC28539), purchased from Bei Na Chuan Lian Bioteknologisk Institut.
It is prepared by bacteria suspension：With the inoculating loop picking 3-4 rings culture Aspergillus flavus spore of 5 days or so, 10ml is placed in aseptic
In the sterilized water of bead, bacterium number is determined using viable bacteria counting method, make cell concentration for 106-107The uniform bacterium of CFU/ml is hanged
Liquid.
Bactericidal test：Take 0.1ml bacteria suspensions and instill Czapek's medium, coating is uniform, as flat board containing bacterium.At experimental group
Reason is as follows：It is respectively that the Aspergillus flavus inhibitor dissolving obtained by embodiment of the present invention 1- embodiment 6 is dilute with sterile deionized water
It is 2% to release to mass percent, by sterilizing filter paper piece (d=6mm) respectively with embodiment of the present invention 1- that mass percent is 2%
Aspergillus flavus inhibitor immersion 1h obtained by embodiment 6, taking-up is affixed on respectively the agar surface containing bacterium flat board of each treatment group
Central authorities.Matched group process is as follows：Sterilizing filter paper piece (d=6mm) is soaked into 1h with sterile deionized water, taking-up is affixed on flat board containing bacterium
Agar surface central authorities.Each experimental group and matched group be respectively provided with 3 it is parallel.The culture dish of each treatment group is placed in into 28 DEG C of bars
Constant temperature culture under part, inhibition zone size is measured after being inverted culture 5 days with slide gauge (precision is 0.02mm), calculates meansigma methodss,
And record.
Represented with "-" without inhibition zone；
Antibacterial circle diameter 7-9mm is slight antibacterial, is represented with "+"；
10～15mm of antibacterial circle diameter is that moderate is antibacterial, is represented with " ++ "；
Antibacterial circle diameter ＞ 15mm are highly antibacterial, are represented with " +++ "；
Experimental result is shown in Table 1：
Inhibition of the Aspergillus flavus inhibitor of the present invention of table 1 to Aspergillus flavus
As shown in Table 1, Aspergillus flavus inhibitor of the invention has significant fungistatic effect to Aspergillus flavus.
Minimum inhibitory concentration is tested
Culture medium：Czapek's medium, heating for dissolving, 121 DEG C of sterilizing 15-20min after subpackage.
Strain is：Aspergillus flavus (ATCC28539), purchased from Bei Na Chuan Lian Bioteknologisk Institut.
It is prepared by bacteria suspension：With the inoculating loop picking 3-4 rings culture Aspergillus flavus spore of 5 days or so, 10ml is placed in aseptic
In the sterilized water of bead, bacterium number is determined using viable bacteria counting method, make cell concentration for 106-107The uniform bacterium of CFU/ml is hanged
Liquid.
By the Aspergillus flavus inhibitor of embodiment of the present invention 1- embodiment 6 carried out respectively with sterile deionized water 50 times, 100
Again, 200 times, 400 times, 800 times, 1000 times, 1500 times of dilutions.Take the culture that 0.1ml bacteria suspensions instill the cooled and solidified that sterilized
In base, coating is uniform, obtains flat board containing bacterium.Each diluent 0.1ml is drawn, the agar table containing bacterium flat board is applied under aseptic condition
Face, be inverted culture 5 days (28 DEG C of cultivation temperature), with do not grow bacterium sample concentration as the minimum antibacterial of Aspergillus flavus inhibitor
Concentration (MIC).Experimental result see the table below (table 2)：
MIC of the Aspergillus flavus inhibitor of the present invention of table 2 to Aspergillus flavus
The above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvement can also be made, these improvement should be regarded as the guarantor of the present invention
Shield scope.
Claims (8)
1. a kind of Aspergillus flavus inhibitor, it is characterised in that：It is prepared from by each component of following masses number：Herba Artemisiae Annuae 20-25
Part, Herba Patriniae 20-25 parts, Radix Arnebiae (Radix Lithospermi) 20-25 parts, Herba Saxifragae 18-25 parts, Flos Lonicerae 17-21 parts, Cortex Fraxini 6-10 parts, Folium Photiniae (Folium Photiniae serrulatae) 15-
20 parts, Flos Bombacis Malabarici 15-20 parts, Rhizoma Curcumae Longae 1-2 parts, Radix Paeoniae Alba 9-12 parts, Radix Bupleuri 7-10 parts, Folium Mori (being boiled into juice) 10-15 parts, Radix Glycyrrhizae 3-
5 parts, Herba Taraxaci 10-15 parts, Pseudobulbus Cremastrae Seu Pleioness 8-12 parts, Rhizoma Curculiginises 2-3 parts, Fructus Piperiss 2-3 parts, Folium Artemisiae Argyi 5-7 parts, Folium Crataegi 6-9 parts.
2. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：20 parts of Herba Artemisiae Annuae, 20 parts of Herba Patriniae, 20 parts of Radix Arnebiae (Radix Lithospermi), 20 parts of Herba Saxifragae, 20 parts of Flos Lonicerae,
9 parts of Cortex Fraxini, 20 parts of Folium Photiniae (Folium Photiniae serrulatae), 20 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, 10 parts of the Radix Paeoniae Alba, 10 parts of Radix Bupleuri, 15 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, Pu Gong
15 parts of English, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss, 6 parts of Folium Artemisiae Argyi, 8 parts of Folium Crataegi.
3. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：21 parts of Herba Artemisiae Annuae, 21 parts of Herba Patriniae, 21 parts of Radix Arnebiae (Radix Lithospermi), 21 parts of Herba Saxifragae, 21 parts of Flos Lonicerae,
6 parts of Cortex Fraxini, 19 parts of Folium Photiniae (Folium Photiniae serrulatae), 19 parts of Flos Bombacis Malabarici, 1 part of Rhizoma Curcumae Longae, 11 parts of the Radix Paeoniae Alba, 9 parts of Radix Bupleuri, 14 parts of Folium Mori, 5 parts of Radix Glycyrrhizae, Pu Gong
14 parts of English, 12 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, 2 parts of Fructus Piperiss, 7 parts of Folium Artemisiae Argyi, 9 parts of Folium Crataegi.
4. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：22 parts of Herba Artemisiae Annuae, 22 parts of Herba Patriniae, 22 parts of Radix Arnebiae (Radix Lithospermi), 22 parts of Herba Saxifragae, 18 parts of Flos Lonicerae,
7 parts of Cortex Fraxini, 18 parts of Folium Photiniae (Folium Photiniae serrulatae), 18 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, 10 parts of the Radix Paeoniae Alba, 9 parts of Radix Bupleuri, 13 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, Pu Gong
13 parts of English, 10 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 5 parts of Folium Artemisiae Argyi, 7 parts of Folium Crataegi.
5. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：23 parts of Herba Artemisiae Annuae, 23 parts of Herba Patriniae, 23 parts of Radix Arnebiae (Radix Lithospermi), 25 parts of Herba Saxifragae, 17 parts of Flos Lonicerae,
7 parts of Cortex Fraxini, 17 parts of Folium Photiniae (Folium Photiniae serrulatae), 17 parts of Flos Bombacis Malabarici, 1 part of Rhizoma Curcumae Longae, 12 parts of the Radix Paeoniae Alba, 8 parts of Radix Bupleuri, 12 parts of Folium Mori, 4 parts of Radix Glycyrrhizae, Pu Gong
12 parts of English, 8 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 6 parts of Folium Artemisiae Argyi, 8 parts of Folium Crataegi.
6. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：24 parts of Herba Artemisiae Annuae, 24 parts of Herba Patriniae, 24 parts of Radix Arnebiae (Radix Lithospermi), 18 parts of Herba Saxifragae, 21 parts of Flos Lonicerae,
10 parts of Cortex Fraxini, 16 parts of Folium Photiniae (Folium Photiniae serrulatae), 16 parts of Flos Bombacis Malabarici, 2 parts of Rhizoma Curcumae Longae, 9 parts of the Radix Paeoniae Alba, 7 parts of Radix Bupleuri, 11 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, Pu Gong
11 parts of English, 9 parts of Pseudobulbus Cremastrae Seu Pleioness, 2 parts of Rhizoma Curculiginises, 3 parts of Fructus Piperiss, 5 parts of Folium Artemisiae Argyi, 9 parts of Folium Crataegi.
7. Aspergillus flavus inhibitor according to claim 1, it is characterised in that：The Aspergillus flavus inhibitor, by following
The each component of mass fraction is prepared from：The Aspergillus flavus inhibitor, is prepared from by each component of following masses number：It is blue or green
25 parts of Artemisia, 25 parts of Herba Patriniae, 25 parts of Radix Arnebiae (Radix Lithospermi), 23 parts of Herba Saxifragae, 19 parts of Flos Lonicerae, 8 parts of Cortex Fraxini, 15 parts of Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici 15
Part, 1 part of Rhizoma Curcumae Longae, 9 parts of the Radix Paeoniae Alba, 7 parts of Radix Bupleuri, 10 parts of Folium Mori, 3 parts of Radix Glycyrrhizae, 10 parts of Herba Taraxaci, 11 parts of Pseudobulbus Cremastrae Seu Pleioness, 3 parts of Rhizoma Curculiginises, Hu
3 parts of green pepper, 7 parts of Folium Artemisiae Argyi, 6 parts of Folium Crataegi.
8. the preparation method of the Aspergillus flavus inhibitor any one of a kind of claim 1-7, it is characterised in that：Including with
Lower step：
(1) each raw material components of the present invention are weighed according to proportioning, and is ground respectively, it is standby；
(2) Herba Artemisiae Annuae, Herba Patriniae, Radix Arnebiae (Radix Lithospermi), Herba Saxifragae, Flos Lonicerae, Cortex Fraxini, Folium Mori are mixed, in being put into marmite, adds 16 times of weight
Clear water, soak at room temperature 48 hours；It is then heated to be cooked by slow fire 30-40 minutes again after boiling, filters to take the filtrate of gained, and
The clear water that weight is 8 times of amounts of filtering residue are added in filtering residue, is heated to being cooked by slow fire 20-30 minutes again after boiling, filter to take institute
The filtrate for obtaining, merging takes the filtrate of gained twice, obtains decoction liquor；
(3) Folium Photiniae (Folium Photiniae serrulatae), Flos Bombacis Malabarici, Rhizoma Curcumae Longae, the Radix Paeoniae Alba, Radix Bupleuri are mixed, is put in ultra cold storage freezer and preserves 12 hours, taken out, treated
It recovers to room temperature to add appropriate distilled water, ultrasonic wave concussion to process 20-25 minutes；
(4) Radix Glycyrrhizae, Herba Taraxaci, Pseudobulbus Cremastrae Seu Pleioness are added in the mixture obtained by step (3) and is mixed, be then placed in agitator often
After temperature concussion 12h, soak under 50-60 DEG C of water bath condition under 2 hours, then 70-80 DEG C of water bath condition and soak 20-30 minutes, filter
Take filtrate；Appropriate distilled water is added in filtering residue, is soaked 20-30 minutes under 70-80 DEG C of water bath condition, filter to take filtrate, merged
Twice the filtrate of gained, obtains soak；
(5) decoction liquor obtained by Folium Artemisiae Argyi, Folium Crataegi and step (2) is mixed, is positioned in the extraction kettle in supercritical extraction instrument,
Utilize carbon dioxide as medium to be extracted, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, is extracted 2 hours, obtains extract；
(6) soak obtained by Rhizoma Curculiginises, Fructus Piperiss and step (4), the extract obtained by step (5) are mixed, then puts agitator
In 12h is shaken with the rotating speed room temperature of 100-150r/min；
(7) by the mixture concentrating under reduced pressure obtained by step (6), the paste stream that density is 1.15～1.20 is surveyed when being concentrated into 60 DEG C
Extractum, bottling.
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2016
- 2016-12-29 CN CN201611242422.5A patent/CN106578390A/en not_active Withdrawn
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| CN115887555A (en) * | 2022-12-01 | 2023-04-04 | 唐山市食品药品综合检验检测中心(唐山市农产品质量安全检验检测中心、唐山市检验检测研究院) | Traditional Chinese medicine composition for inhibiting aspergillus flavus |
| CN115887555B (en) * | 2022-12-01 | 2024-04-26 | 唐山市食品药品综合检验检测中心(唐山市农产品质量安全检验检测中心、唐山市检验检测研究院) | Traditional Chinese medicine composition for inhibiting aspergillus flavus |
| CN117281884A (en) * | 2023-11-24 | 2023-12-26 | 山东天宇生物科技有限公司 | Traditional Chinese medicine composition for preventing chicken aflatoxin poisoning and preparation process thereof |
| CN117281884B (en) * | 2023-11-24 | 2024-03-01 | 山东畜牧兽医职业学院 | Traditional Chinese medicine composition for preventing chicken aflatoxin poisoning and preparation process thereof |
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