CN106577261A - Method for selecting and breeding photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility - Google Patents

Method for selecting and breeding photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility Download PDF

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Publication number
CN106577261A
CN106577261A CN201611157743.5A CN201611157743A CN106577261A CN 106577261 A CN106577261 A CN 106577261A CN 201611157743 A CN201611157743 A CN 201611157743A CN 106577261 A CN106577261 A CN 106577261A
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China
Prior art keywords
breeding
mamamama
photoperiod
generation
cenospecies
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CN201611157743.5A
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Chinese (zh)
Inventor
赵威军
张福耀
张阳
邵荣峰
常玉卉
卜华虎
张伟
李金梅
王花云
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Sorghum Institute Shanxi Academy Of Agricultural Sciences
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Sorghum Institute Shanxi Academy Of Agricultural Sciences
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Priority to CN201611157743.5A priority Critical patent/CN106577261A/en
Publication of CN106577261A publication Critical patent/CN106577261A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to a method for selecting and breeding a photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility. According to the method, because a sorghum strain mamaMaMa (EBA-3) has no photoperiod-sensitive characteristic but the cross bred F1 generation has a photoperiod-sensitive sterile characteristic, the mamaMaMa is improved, a stain with an mamaMaMa characteristic and a higher sugar content is selected and bred, then test crossing is conducted, and the photoperiod-sensitive sterile sweet sorghum hybrid variety is obtained. The photoperiod-sensitive sterile sweet sorghum hybrid variety can be obtained by means of the method, and the hybrid variety cannot ear and produce fruits, is high in biological yield and high in lodging resistance, the sugar content of the stalks is high, and a variety support can be provided for industrialization of sweet sorghum.

Description

A kind of method using the photosensitive sterile sugar grass cenospecies of photosensitive sterility selection-breeding
Technical field
The present invention relates to crop breeding field, more particularly to it is a kind of miscellaneous using the photosensitive sterile sugar grass of photosensitive sterility selection-breeding Hand over the method planted.
Background technology
Sugar grass is increasingly subject to people's attention as emerging sugar material, feedstuff and energy crop, increasingly causes one A little national governments and the concern of international organization.But the energy Sweet Sorghum Breeding research work of China is relatively fewer, existing sweet height Fine strain of millet kind sugar content only has 16～18 ° (BX), and lodging resistance is poor, far from adaptation energy Sweet Sorghum Industry process requirements.
Creator of the present invention introduces Sorghum vulgare Pers. strain EBA-3 in 2010 in Sorghum Breeding research (ma5ma5Ma5Ma5), this research department is named as mamaMaMa, and mamaMaMa does not show light sensitive characteristic, but which is miscellaneous with other strains Hand over F1 generation to show light sensitive characteristic, could ear when illumination is less than 0 minute 12 hours and bloom.
If this characteristic is used for selection-breeding sugar grass cenospecies, sugar grass cenospecies will be with two big advantages.One is to improve Sugar grass lodging tolerance.It is due to the photosensitive infertility of cenospecies, not solid, thus it is possible to vary period of maturation sugar grass is top-heavy existing As lower the center of gravity height, improves lodging tolerance.Two is to improve sweet broomcorn straw sugar content.Due to the photosensitive infertility of cenospecies, do not have Have solid, photosynthate can not convert storage to seed, can only be stored in cane, such that it is able to significantly improve cenospecies Cane sugar content.
Jing tests determine cenospecies A302427 × Kazakhstan T-1/mamaMaMa-1 is opened, cenospecies are in Shanxi Jinzhong city to October Can not ear within 13rd and bloom, show photosensitive sterile gene, and stalk yield is high, sugared brix is high and stalk crushing juice rate is high, resists Lodging performance is strong.
In view of Sweet Sorghum Breeding reality, through years of researches and practice, creator of the present invention obtains this creation finally.
The content of the invention
It is an object of the invention to provide a kind of method using the photosensitive sterile sugar grass cenospecies of photosensitive sterility selection-breeding, Comprise the steps：
(1) maternal selection-breeding
Using Sorghum vulgare Pers. strain A3R111 is A3Cytoplasm donor, it is cell nucleus donor (i.e. samsara male parent) to open 02427, continuously In 5 generation of samsara transformation, produces female parent A3Open 02427.
(2) male parent selection-breeding
1. in China Hainan plantation mamaMaMa and Kazakhstan T-1 materials, emasculation hybridization is carried out, F is produced1Generation.
2. F is planted in Hainan1In generation, bagging selfing, produce F2Generation.
3. plant F2In generation, bagging selfing, select similar mamaMaMa, energy normal mature, the individual plant of stalk succulence polysaccharide to receive Obtain；According to said method, 3 generation of continuous selfed breeding, selection-breeding obtain similar mamaMaMa, can normal mature, stalk succulence polysaccharide it is new Strain breathes out T-1/mamaMaMa-1.
(3) cenospecies assemble and identify
With A302427 is opened for female parent, Kazakhstan T-1/mamaMaMa-1 is male parent, assembles cenospecies, and cenospecies are reflected It is fixed.
The invention has the advantages that：
Using two Sorghum vulgare Pers. incrosses, F1For the high characteristic of photosensitive infertility, biological yield, male parent is improved, is selected Photosensitive infertility, high yield, high sugared, anti-fall sugar grass cenospecies are educated, so that kind support is provided for Sweet Sorghum Industry.
Description of the drawings
Fig. 1 is a kind of stream of the method using the photosensitive sterile sugar grass cenospecies of photosensitive sterility selection-breeding of the embodiment of the present invention Cheng Tu.
Flow charts of the Fig. 2 for the embodiment of the present invention.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, with reference to embodiments the present invention is carried out further Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention It is bright.
As shown in figure 1, embodiments providing a kind of using the photosensitive sterile sugar grass hybridization of photosensitive sterility selection-breeding The method planted, comprises the steps：
(1) maternal selection-breeding
Using Sorghum vulgare Pers. strain A3R111 is A3Cytoplasm donor, it is cell nucleus donor (i.e. samsara male parent) to open 02427, continuously In 5 generation of samsara transformation, produces female parent A3Open 02427.
(2) male parent selection-breeding
1. in China Hainan plantation mamaMaMa and Kazakhstan T-1 materials, emasculation hybridization is carried out, F is produced1Generation.
2. F is planted in Hainan1In generation, bagging selfing, produce F2Generation.
3. plant F2In generation, bagging selfing, select similar mamaMaMa, energy normal mature, the individual plant of stalk succulence polysaccharide Harvest；According to said method, 3 generation of continuous selfed breeding, selection-breeding obtain similar mamaMaMa, can normal mature, stalk succulence polysaccharide New lines breathe out T-1/mamaMaMa-1.
(3) cenospecies assemble and identify
With A302427 is opened for female parent, Kazakhstan T-1/mamaMaMa-1 is male parent, assembles cenospecies, and cenospecies are reflected It is fixed.
Embodiment
As shown in Fig. 2 the Breeding Process of photosensitive sterile sugar grass cenospecies is obtained using the method.
Winter in 2011 breathes out T-1 and mamaMaMa materials in the plantation of China Hainan, florescence to breathing out T-1 artificial emasculations, with MamaMaMa hybridizes, and produces F1In generation, (breathes out T-1 × mamaMaMa).
Winter in 2012 breathes out T-1 × mamaMaMa, F in Hainan plantation combination1For bagging selfing, 3 fringes are received in choosing, produce F2Generation.
F is planted in Yuci Distrct of Jinzhong City within 20132In generation, florescence bagging selfing, harvest similar mamaMaMa, energy The individual plant of normal mature, stalk succulence polysaccharide, i.e. F3Generation.
F is planted in Yuci within 20143In generation, florescence bagging selfing, harvest similar mamaMaMa, energy normal mature, stalk The individual plant of succulence polysaccharide, i.e. F4Generation.
F is planted in Yuci within 20154In generation, florescence bagging selfing, harvest similar mamaMaMa, energy normal mature, stalk The individual plant of succulence polysaccharide.A is planted simultaneously302427 is opened, paternal pollen is taken and is assembled cenospecies A3Open 02427 × Kazakhstan T-1/ mamaMaMa-1。
2016 to cenospecies A3Open 02427 × Kazakhstan T-1/mamaMaMa-1 and identified that the cenospecies biological yield is high, Nutrient matter is high, and lodging resistance is strong.
In sum, originally it is embodied as using the photosensitive Sorghum vulgare Pers. strain of improvement, F1For the high spy of photosensitive infertility, biological yield Property, Sorghum vulgare Pers. male parent is improved, selection-breeding to photosensitive infertility, high yield, high sugared, anti-fall sugar grass cenospecies, so as to for sugar grass Industrialization provides kind and supports.
Presently preferred embodiments of the present invention is the foregoing is only, is merely illustrative for invention, and it is nonrestrictive. Those skilled in the art understanding, can carry out many changes in the spirit and scope limited by invention claim to which, change, It is even equivalent, but fall within protection scope of the present invention.

Claims (2)

1. a kind of method using the photosensitive sterile sugar grass cenospecies of photosensitive sterility selection-breeding, comprises the steps：
(1) maternal selection-breeding
Using Sorghum vulgare Pers. strain A3R111 is A3Cytoplasm donor, opens 02427 for cell nucleus donor (i.e. samsara male parent), continuous samsara In 5 generation of transformation, produces female parent A3Open 02427.
(2) male parent selection-breeding
1. in China Hainan plantation mamaMaMa and Kazakhstan T-1 materials, emasculation hybridization is carried out, F is produced1Generation.
2. F is planted in Hainan1In generation, bagging selfing, produce F2Generation.
3. plant F2In generation, bagging selfing, select similar mamaMaMa, energy normal mature, the individual plant of stalk succulence polysaccharide to harvest；Press The method, 3 generation of continuous selfed breeding, selection-breeding obtain similar mamaMaMa, energy normal mature, the new lines of stalk succulence polysaccharide and breathe out T-1/mamaMaMa-1。
(3) cenospecies assemble and identify
With A302427 is opened for female parent, Kazakhstan T-1/mamaMaMa-1 is male parent, assembles cenospecies, and cenospecies are identified.
2. a kind of method using the photosensitive sterile sugar grass cenospecies of photosensitive sterility selection-breeding according to claim 1, its It is characterised by, obtained sugar grass hybridizes to photoperiod-sensitive, can not ear in China the north, biological yield is high, stalk is containing sugar Amount is high, and lodging resistance is strong.
CN201611157743.5A 2016-12-08 2016-12-08 Method for selecting and breeding photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility Pending CN106577261A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679477A (en) * 2019-11-15 2020-01-14 河北省农林科学院旱作农业研究所 Breeding method of photoperiod strong-sensitivity BMR sorghum sterile line
CN113973708A (en) * 2021-11-26 2022-01-28 河北省农林科学院旱作农业研究所 Method for judging whether forage grass sorghum strain has photo-thermo-sensitive sterility characteristic

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CN1276149A (en) * 1999-06-03 2000-12-13 湖南省农业科学院高粱研究开发中心 Two-line hybridization method for producing sorghum seeds
CN103461091A (en) * 2013-07-12 2013-12-25 山西省农业科学院高梁研究所 Seed selection method of sorghum A3 cytoplasmic male sterile line
CN103651099A (en) * 2013-11-27 2014-03-26 中兴能源(内蒙古)有限公司 Sweet sorghum sterile line breeding method
CN104521735A (en) * 2014-11-28 2015-04-22 山西省农业科学院高粱研究所 Method for creating sorghum A3 cytoplasmic male sterility restorer line
CN105349573A (en) * 2015-12-05 2016-02-24 山西省原平市双惠种业有限公司 Sorghum variety breeding and cultivating method

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Publication number Priority date Publication date Assignee Title
CN1276149A (en) * 1999-06-03 2000-12-13 湖南省农业科学院高粱研究开发中心 Two-line hybridization method for producing sorghum seeds
CN103461091A (en) * 2013-07-12 2013-12-25 山西省农业科学院高梁研究所 Seed selection method of sorghum A3 cytoplasmic male sterile line
CN103651099A (en) * 2013-11-27 2014-03-26 中兴能源(内蒙古)有限公司 Sweet sorghum sterile line breeding method
CN104521735A (en) * 2014-11-28 2015-04-22 山西省农业科学院高粱研究所 Method for creating sorghum A3 cytoplasmic male sterility restorer line
CN105349573A (en) * 2015-12-05 2016-02-24 山西省原平市双惠种业有限公司 Sorghum variety breeding and cultivating method

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679477A (en) * 2019-11-15 2020-01-14 河北省农林科学院旱作农业研究所 Breeding method of photoperiod strong-sensitivity BMR sorghum sterile line
CN110679477B (en) * 2019-11-15 2021-06-01 河北省农林科学院旱作农业研究所 Breeding method of photoperiod strong-sensitivity BMR sorghum sterile line
CN113973708A (en) * 2021-11-26 2022-01-28 河北省农林科学院旱作农业研究所 Method for judging whether forage grass sorghum strain has photo-thermo-sensitive sterility characteristic

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