CN106577218B - Method for inhibiting growth of lateral roots of Malus hupehensis Rehd - Google Patents
Method for inhibiting growth of lateral roots of Malus hupehensis Rehd Download PDFInfo
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- CN106577218B CN106577218B CN201610980613.5A CN201610980613A CN106577218B CN 106577218 B CN106577218 B CN 106577218B CN 201610980613 A CN201610980613 A CN 201610980613A CN 106577218 B CN106577218 B CN 106577218B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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Abstract
The invention discloses a method for inhibiting the growth of lateral roots of Malus hupehensis Rehd, which comprises the steps of transplanting Malus hupehensis Rehd seedlings into 1/2Hoagland hydroponic nutrient solution; the MeJA treatment concentration is 0.2mg/L, normal management is carried out after one week of treatment, the nutrient solution is changed once a week, and the culture is carried out for one month. The MeJA treatment inhibits the number of lateral roots, plant height and stem thickness of the Malus hupehensis seedling, the root length, root surface area and root volume are reduced by 0-7 days after the MeJA treatment, and the values of the MeJA treatment and the MeJA treatment are increased by 14-28 days; MeJA inhibits lateral root development, reducing the number of lateral roots; providing a certain theoretical basis for the development of apple lateral roots; the method has very important significance for guiding stock breeding and improving cultivation measures in actual production; inhibition of lateral root development by MeJA treatment may reduce environmental damage. After MeJA treatment, the growth amount of lateral roots is inhibited, and the overall activity of the root system and the utilization rate of water and nutrients are inhibited.
Description
Technical Field
The invention belongs to the technical field of commercial crop planting, and particularly relates to a method for inhibiting growth of lateral roots of Malus hupehensis Rehd.
Background
The plant root system is an important organ for absorbing water and nutrition, and the lateral root is an important component of the plant root system. The research history of lateral root development is long, most of the research is concentrated on the herbaceous plant arabidopsis thaliana (Meyerowitz, 2001), however, in woody plants, especially in economic crops, the research on lateral root development is delayed, and the development of practical work such as breeding of economic crops and the like targeting the excellent property of root systems is difficult to a certain extent. It is well known that lateral root development and development are affected by both endogenous plant hormones and external environmental factors. Among the five major classes of classical plant hormones, in additionThe source auxin promotes lateral root generation (Benkova et al 2009; Fukaki et al 2009); exogenous cytokinins and abscisic acid inhibit the generation of lateral roots; gibberellins have no or little effect; ethylene plays a role in regulating the metabolism of auxin (queen tree et al, 2003), and thus most of the hormones reported to occur in lateral roots are the regulation of lateral root formation by auxin. Peng and li seaboat (1989) found that methyl jasmonate (MeJA) is a type of plant growth regulator and inhibits growth of peanut hypocotyls and roots (binjin hua and peng, 1998). Huangshengqin et al (2002) found high concentration (10)-6-10-4mol/L) of methyl jasmonate (MeJA) inhibited the growth of peanut root and hypocotyl. There are still many problems still unclear about the occurrence of lateral roots and their hormonal regulation, and Jasmonic Acid (JA) has especially been reported only rarely on the mechanism of lateral root development. Besides hormones, environmental conditions such as light, temperature and moisture, cultivation measures and varieties are also closely related to the development of lateral roots. Existing techniques for inhibiting lateral root development have focused on heavy metals including: the inhibition effect of aluminum, copper, zinc, cadmium and the like on the development of lateral roots (Poplar et al, 2013; Wangmitao et al, 2009; Xuming gang et al, 2008), and the heavy metal ions have great harm to the safety of soil environment.
In conclusion, the existing technologies for inhibiting the development of lateral roots focus on heavy metal ions, which are harmful to the environment and not beneficial to the normal growth of plants.
Disclosure of Invention
The invention aims to provide a method for inhibiting the lateral root development of Malus hupehensis Rehd, and aims to solve the problems that the existing technology for inhibiting the lateral root development concentrates on heavy metal ions, is harmful to the environment and is not beneficial to the normal growth of plants.
The invention is realized in such a way, the method for inhibiting the growth of the lateral roots of the Malus hupehensis Rehd is characterized in that the Malus hupehensis Rehd seedling is transplanted into 1/2Hoagland water culture nutrient solution; the MeJA treatment concentration is 0.2mg/L, normal management is carried out after one week of treatment, the nutrient solution is changed once a week, and the culture is carried out for one month.
Further, before transplanting, the Malus hupehensis Rehd seedlings are planted after being stored in sand for one month, 8-10 leaves of 60 d-old Malus hupehensis Rehd seedlings without diseases and insect pests are selected and taken out of a nutrition pot, rinsed with clear water to remove a matrix, and transplanted into a greenhouse.
Further, the temperature of the greenhouse in 12h of light every day is 25 +/-1 ℃, the temperature of the greenhouse in 12h of dark is 15 +/-1 ℃, and the average humidity is 70-80%.
Further, the hydroponic nutrient solution is changed once a week.
Further, the 1/2Hoagland nutrient solution comprises Ca (NO)3)2·4H2O 410mg/L、KNO3252.5mg/L、KH2PO4·2H2O 68mg/L、MgSO4·7H2O 246.5mg/L、FeSO4·7H2O 350mg/L、EDTA-Na 465mg/L、H3BO31.43mg/L、MnCI·4H2O 0.905mg/L、ZnSO4·7H2O 0.11mg/L、CuSO4·5H2O0.04 mg/L and H2MoO4·H2O0.01 mg/L.
Further, the 1/2Hoagland nutrient solution preparation method comprises the following steps: preparing 400 times of mother solution according to the components of 1/2Hoagland nutrient solution, and adding the required nutrient element content according to the volume of a plastic basin of a water culture device.
The invention also aims to provide a method for utilizing the method for inhibiting the lateral root development of the Malus hupehensis Rehd to discover a physiological mechanism for regulating the lateral root development by using the plant growth regulator.
According to the method for inhibiting the lateral root development of Malus hupehensis Rehd, the MeJA treatment inhibits the number of the lateral roots, the plant height and the stem thickness of Malus hupehensis Rehd seedlings, the root length, the root surface area and the root volume are reduced by 0-7 days after the MeJA treatment, the values of the MeJA treatment and the MeJA treatment are increased, the MeJA inhibits the lateral root development, and particularly the number of the lateral roots is reduced; can provide a certain theoretical basis for the development of lateral roots of apples, and has very important significance for guiding stock breeding and improving cultivation measures in actual production. Whereas MeJA treatment to inhibit lateral root development may reduce environmental damage. After MeJA treatment, the growth amount of lateral roots is inhibited, and the overall activity of the root system and the utilization rate of water and nutrients are inhibited. The apple is the king of the temperate zone apple and is an important economic crop, the Malus hupehensis Rehd is used as a precious and scarce apple stock resource in China, and the Malus hupehensis Rehd has very important agricultural significance for researching the root development of the Malus hupehensis Rehd by taking the Malus hupehensis Rehd as a material.
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FIG. 1 is a flowchart of a method for inhibiting the lateral root development of Malus hupehensis according to an embodiment of the present invention.
Fig. 2 is a schematic diagram of statistics of lateral root number, root length, root surface area and root volume of the hupi-sinensis MeJA after the processing.
FIG. 3 is a statistical representation of plant height and stem thickness of MeJA treated Malus hupehensis seedlings provided by the embodiments of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The following detailed description of the principles of the invention is provided in connection with the accompanying drawings.
As shown in fig. 1, the method for inhibiting lateral root development of hupehensis rehd according to the embodiment of the present invention includes the following steps:
s101, planting the Malus hupehensis Rehd seeds in a nutrition pot after the Malus hupehensis Rehd seeds are stored for one month in a sand mode, carrying out normal management, selecting 8-10 leaves of 60 d-seedling-old Malus hupehensis Rehd seedling without diseases and insect pests, taking out the seedling-old Malus hupehensis Rehd seedlings from the nutrition pot, rinsing with clear water to remove a matrix, and transplanting the seedling-old Malus hupehensis Rehd seedlings into a greenhouse, wherein the illumination temperature of the greenhouse every 12 hours is 25 +/-1 ℃, the dark temperature of the greenhouse every 12 hours is 15 +/-1 ℃, and the;
s102: transplanting the seedlings of the Malus hupehensis Rehd into 1/2Hoagland water culture nutrient solution for seedling recovery for half a month, and changing the nutrient solution once a week;
s103: the MeJA treatment concentration is 0.2mg/L, normal management is carried out after one week of treatment, the nutrient solution is changed once a week, and the culture is carried out for one month.
The principle of the invention is further described below in connection with comparative experiments.
In order to know the influence of exogenous methyl jasmonate (MeJA) on the development of lateral roots, the Malus hupehensis Rehd seedling is treated by exogenous 0.2mg/L MeJA, and indexes such as the number of roots, the length of the roots, the surface area of the roots, the volume of the roots, the plant height, the stem thickness and the like are investigated in the lateral root development process. The results show that MeJA treatment inhibits the lateral root number, plant height and stem thickness of the Malus hupehensis seedling, the root length, root surface area and root volume are reduced after 0-7 days of MeJA treatment, the values of MeJA treatment and MeJA treatment are increased after 14-28 days of MeJA treatment, and MeJA inhibits the lateral root growth, particularly reduces the lateral root number. Can provide a certain theoretical basis for the development of lateral roots of apples, and has very important significance for guiding stock breeding and improving cultivation measures in actual production.
1. Experimental materials and treatments
The Malus hupehensis Rehd has the smallest individual difference and the best consistency in common apple stocks, the root system of the Malus hupehensis Rehd is most sensitive to the change of the soil environment, and the variation of the overground part of the plant and the variation of the root system have obvious correlation; meanwhile, because of the high apomixis capacity, the phenotype and the genotype of the Malus hupehensis Rehd are unified, Malus hupehensis Rehd root is taken as a research material, the risk that the treatment effect is covered by gene difference can be avoided, and the Malus hupehensis Rehd has strong waterlogging tolerance, is easy to perform solution culture and is beneficial to controlling the test condition through water culture. Therefore, the Malus hupehensis Rehd is an excellent apple root system research material.
2. Experimental treatment:
3-5 days in 2015, planting Malus hupehensis seeds in the experimental station of northwest Yangling agriculture and forestry university (108 degrees 04 'E and 34 degrees 16' N) after sand storage for one month, selecting 8-10 leaves of 60 d-seedling-age Malus hupehensis seedlings without diseases and insect pests, taking out the seedlings from the nutrition pot, rinsing with clear water to remove a matrix, transplanting the seedlings into a scientific research greenhouse, wherein the illumination temperature of the greenhouse is 25 +/-1 ℃ for 12h every day, the dark temperature of the greenhouse is 15 +/-1 ℃ for 12h, and the average humidity is 70-80%. Transplanting the seedlings of the Malus hupehensis Rehd into 1/2Hoagland water culture nutrient solution for seedling recovery for half a month, changing the nutrient solution once a week, wherein the formula of the 1/2Hoagland nutrient solution is shown in Table 1. The water culture device is a square imperforate plastic basin (50cm multiplied by 35cm multiplied by 15cm), the periphery of the plastic basin is covered by black plastic paper, polystyrene foam is used as a planting plate, 30 holes are drilled in each foam plate, the Pingyi sweet tea seedlings are placed, the stems are surrounded and positioned by sponge, the roots are immersed in the basin, 30 plants are planted in each container, and air is introduced into each plastic basin by an air pump.
Exogenous MeJA and control treatments were set, methyl jasmonate was purchased from Simian Watson Bioreagent, Inc. at a concentration of 0.2mg/L, and 90 of each of the control and hormone treatments were 180. After one week of treatment, the nutrient solution is changed once a week for normal management, and the culture is carried out for one month.
3. Sampling time: samples were taken on days 0, 3, 7, 14, 21, and 28 after nutrient solution treatment, and 15 samples were taken for control and each hormone treatment, three biological replicates, and a total of six samples were taken.
4. The determination method comprises the following steps: a root system scanner is used for collecting root system images after sampling every time, the model is LAl600, root system analysis software WinRHIO is used for analyzing root system data, and relevant measured indexes comprise root length, root surface area and root volume. In addition, the plant height was measured with a ruler and the stem thickness was measured with a vernier caliper.
FIG. 2 statistics of root number, root length, root surface area, and root volume after MeJA treatment of Malus hupehensis Rehd. p is <0.05, which indicates that p is <0.01
The 1/2Hoagland nutrient solution comprises: ca (NO)3)2·4H2O 410mg/L、KNO3 252.5mg/L、KH2PO4·2H2O 68mg/L、MgSO4·7H2O 246.5mg/L、FeSO4·7H2O 350mg/L、EDTA-Na 465mg/L、H3BO31.43mg/L、MnCI·4H2O 0.905mg/L、ZnSO4·7H2O 0.11mg/L、CuSO4·5H2O 0.04mg/L、H2MoO4·H2O 0.01mg/L。
Further, the preparation method is that the mother solution with the volume of 400 times is prepared according to the 1/2Hoagland nutrient solution formula, and the required nutrient element content in the formula is added according to the volume of the plastic basin of the water culture device.
FIG. 3 shows the statistics of plant height and stem thickness of MeJA treated Pingyi sweet tea seedlings
TABLE 11/2 Hoagland nutrient solution formula
Table 2 is divided according to root diameter: statistics of root length, root volume, root surface area for four root system grades <0.5mm, 0.5-2mm, 2-5mm and >5mm
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (3)
1. A method for regulating and controlling the development of lateral roots of Malus hupehensis Rehd is characterized in that Malus hupehensis Rehd seedlings are transplanted into 1/2Hoagland water culture nutrient solution by the method for regulating and controlling the development of the lateral roots of Malus hupehensis Rehd; the MeJA treatment concentration is 0.2mg/L, normal management is carried out after treatment for one week, the nutrient solution is changed once per week, and cultivation is carried out for one month;
before transplanting, planting the Malus hupehensis seedlings, namely, storing Malus hupehensis seeds in sand for one month, selecting 8-10 leaves of 60d old Malus hupehensis seedlings without diseases and insect pests, taking out the 60d old Malus hupehensis seedlings from a nutrition pot, rinsing with clear water to remove a matrix, and transplanting the seedlings into a greenhouse;
the illumination temperature of the greenhouse is 25 +/-1 ℃ in 12h every day, the dark temperature of the greenhouse is 15 +/-1 ℃ in 12h, and the average humidity is 70-80%;
the hydroponic nutrient solution is changed once a week;
the method for regulating and controlling the lateral root development of Malus hupehensis Rehd uses MeJA, the treatment concentration of MeJA is 0.2mg/L, the lateral root growth is inhibited 0-7 days after the MeJA treatment, the number of the lateral roots is reduced, and the lateral root growth is promoted 14-28 days later.
2. The method of claim 1, wherein the growth of lateral roots of Malus hupehensis Rehd is regulatedThe 1/2Hoagland nutrient solution consists of Ca (NO)3)2·4H2O 410mg/L、KNO3 252.5mg/L、KH2PO4·2H2O 68mg/L、MgSO4·7H2O 246.5mg/L、FeSO4·7H2O 350mg/L、EDTA-Na 465mg/L、H3BO3 1.43mg/L、MnCI·4H2O 0.905mg/L、ZnSO4·7H2O 0.11mg/L、CuSO4·5H2O0.04 mg/L and H2MoO4·H2O0.01 mg/L.
3. The method for regulating and controlling the development of lateral roots of Malus hupehensis Rehd as claimed in claim 2, wherein the 1/2Hoagland nutrient solution is prepared by the following steps: preparing 400 times of mother solution according to the components of 1/2Hoagland nutrient solution, and adding the required nutrient element content according to the volume of a plastic basin of a water culture device.
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| CN104684871A (en) * | 2012-09-27 | 2015-06-03 | 诺维信生物农业公司 | Compositions and methods for enhancing plant growth |
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| 茉莉酸甲酯（JA-Me)对莴苣幼苗侧根原基形成及内源激素的影响;管晓春等;《江苏农业研究》;19990131;第20卷(第1期);第26-29页 * |
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