CN106568939B - Chemiluminescence immunoassay detection reagent item and its application for detecting pesticide residue of veterinary drug - Google Patents
Chemiluminescence immunoassay detection reagent item and its application for detecting pesticide residue of veterinary drug Download PDFInfo
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- CN106568939B CN106568939B CN201610933988.6A CN201610933988A CN106568939B CN 106568939 B CN106568939 B CN 106568939B CN 201610933988 A CN201610933988 A CN 201610933988A CN 106568939 B CN106568939 B CN 106568939B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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Abstract
The present invention is provided to detect the chemiluminescence immunoassay detection reagent item of pesticide residue of veterinary drug, it is pasted onto successively on PVC liner plates (7) including sample pad (1), the glass fibre membrane for the detected material for being adsorbed with enzyme label or polyamides ester film (2), the nitrocellulose filter (3) for being fixed with detected material antibody, luminous agent film (4), urea peroxide film (5) and absorption pad (6), more than each section.During measure, adsorbed on the sample to be tested and the glass fibre membrane or polyamides ester film being added dropwise using in sample pad enzyme label detected material as move phase, the luminous zone being made of detected material antibody fixed on the nitrocellulose filter, luminous agent film and urea peroxide film, as stationary phase, luminous zone institute's luminous intensity is in corresponding proportionate relationship with the content of determinand in sample, by light sensing detector, measurement result is directly displayed.The reagent strip of the present invention provides quick, simple and direct, accurate detection means for quantitatively detection Pesticide and residue of veterinary drug.
Description
Technical field
The invention belongs to the technical field of biological in biomedicine field, specifically, being related to a kind of chemiluminescence
Immunologic function test reagent item and its application for detecting pesticide residue of veterinary drug.
Background technology
Current detection technique mainly includes two developing direction, one is the inspection technology side automatically controlled to large-scale precision
Face is developed, and makes inspection technology standardization, precision, intensive degree higher and higher, be second to economic, quick, universalness,
Develop in terms of popular detection technique, to meet the needs of public self health care, self-protection consciousness.With regard to food security
Speech, the supervision project of national regulation, pesticide have 136 kinds, and veterinary drug has 217 kinds.In face of so many detection type, inspection how is improved
Efficiency, quantitative and easy detection Pesticide and residue of veterinary drug are surveyed, is always a urgent problem to be solved.
Quickly detected currently for pesticide veterinary drug, using it is most be " colloidal gold immunochromatographimethod reagent strip ".The reagent strip
It using antibody and the sample liquid of colloid gold particle absorption is mobile phase to be, determinand (antigen) in sample and to be fixed on nitric acid fine
Tie up the antigen (stationary phase) on film, common competition is combined with antibody, and antigen is more in sample, combined with antibody in colloidal gold it is more, then
It is deposited on that antibody in stationary phase is few, in the position of stationary phase, the red line of display is shallow.On the contrary, determinand is few in sample, display
Red line is deep.To visually observe the red depth, come judge residue number, if it is exceeded.This method is easy, quickly.But it ties
Fruit is judged according to range estimation, often equivocal.Colloid gold reagent item can only be used for qualitative, positive-exceeded, feminine gender-not exceeded,
Using there is certain limitation.The detection limit needed for detection cannot be reached under some cases.It there is now " colloidal-gold strip chart scanner ", lead to
It crosses and emits light on film, read the intensity of reflected light to measure the stationary phase red depth, the amount of residue is quantitative determined with this.
But instrument detects intensity of reflected light, sensitivity, accuracy have the problem of certain by emitting refraction of the light on film.
Invention content
The object of the present invention is to provide a kind of chemiluminescence immunoassay detection reagent item and its in pesticide residue of veterinary drug is detected
Application.
In order to realize the object of the invention, the present invention is used to detect the chemiluminescence immunoassay detection reagent of pesticide residue of veterinary drug
Item including sample pad 1, the glass fibre membrane for the detected material (enzyme-labelled antigen) for being adsorbed with enzyme label or polyamides ester film 2, is fixed with
Nitrocellulose filter (NC films) 3, luminous agent film 4, urea peroxide film 5, absorption pad 6 and the PVC liner plates 7 of detected material antibody.
The sample pad 1, is fixed with detected material antibody at the glass fibre membrane 2 for being adsorbed with the detected material that enzyme marks
Nitrocellulose filter 3, the luminous agent film 4 and absorption pad 6 are pasted onto successively on PVC liner plates 7；Wherein, the urea peroxide film 5
The lower section of the nitrocellulose filter 3 for being fixed with detected material antibody is arranged on, it is opposite with the position of the luminous agent film 4
It should.Reagent strip structure is as shown in Figure 1.
The long 40-60mm of PVC liner plates, wide 3-4mm；The long 8-10mm of sample pad, wide 3-4mm, material are selected from polyamides
Ester, glass fibre or filter paper；It is described to be adsorbed with the glass fibre membrane of detected material of enzyme label or polyamides ester film long 3-5mm, it is wide
3-4mm, the enzyme are horseradish peroxidase；The nitrocellulose filter long 20-30mm for being fixed with detected material antibody, it is wide
3-4mm, the antibody are monoclonal antibody or polyclonal antibody；The antibody is fixed on it close to sample pad side cellulose nitrate
At plain film 6-10mm；The long 2-5mm of luminous agent film, wide 3-4mm, material are selected from polyamides ester, glass fibre or filter paper, hair used
Photo etching is luminol or derivatives thereof；The long 2-5mm of urea peroxide film, wide 3-4mm, material are selected from polyamides ester, glass fibre
Or filter paper, wherein urea peroxide can be replaced with peroxide such as perbenzoic acids；The long 12-15mm of absorption pad, wide 3-
4mm, material are selected from filter paper material.
The sample pad is adsorbed with 1- on the glass fibre membrane of detected material of enzyme label or polyamides ester film described in being pressed in
2mm, the glass fibre membrane or polyamides ester membrane pressure of the detected material for being adsorbed with enzyme label resist in the detected material that is fixed with
3-4mm on the nitrocellulose filter of body, the nitrocellulose filter other end for being fixed with detected material antibody is apart from edge 5-
Paste the luminous agent film at 7mm, the absorption pad, which is pressed in, described is fixed with 2- on the nitrocellulose filter of detected material antibody
3mm。
The glass fibre membrane or polyamides ester film of the detected material for being adsorbed with enzyme label are that enzyme label detected material is molten
Liquid is sprayed at after glass fibre membrane or polyamides ester film through being dried to obtain；
The nitrocellulose filter for being fixed with detected material antibody is that detected material antibody-solutions are sprayed at nitric acid fibre
Through being dried to obtain after the plain film of dimension.
The luminous agent film be by a concentration of 0.5-10.0mM luminols or derivatives thereof solution spraying on film through drying
It obtains；Wherein, the sodium carbonate-bicarbonate buffering of described luminol or derivatives thereof solution 0.1-1%, pH8-9.5 is molten
Liquid is prepared；
The urea peroxide film is after soaking film with the urea peroxide solution of a concentration of 10%-30%, through what is be dried to obtain.
The detected material includes various antibiotic, steroid hormone and pesticide.Preferably, the detected material includes chlorine
Mycin, gentamicin, progesterone, testosterone, chloramines spirit, more prodigiosins and each type organic that associated antibodies can be obtained etc..
The present invention also provides the changes for being used to detect pesticide residue of veterinary drug containing the chemiluminescence immunoassay detection reagent item
Learn electrochemiluminescent immunoassay detection kit, by the reagent strip be placed in a plastics get stuck it is middle assembling obtain.
It is described to get stuck including box body and cover board, it is parallel on the bottom plate of the box body to be equipped with two identical grooves of size, it is recessed
The chemiluminescence immunoassay detection reagent item and contrast agents item are placed in slot respectively；There is ridge to separate it between two slots, close the lid
During plate, tray interior is separated into two lighttight spaces.Reagent cartridge configuration is as shown in Figure 2.
Wherein, the contrast agents item is to resist the detected material that is fixed on the chemiluminescence immunoassay detection reagent item
The nitrocellulose filter of body is substituted for what blank nitrocellulose filter obtained.
Described cover board one end is equipped at least one well, corresponding to the sample pad of the reagent strip；The cover board is another
The rapid parallel position for being equipped with two lightening holes, corresponding respectively to luminous agent film on the reagent strip.It optionally can be in the well
At least one observation window is equipped between lightening hole, for monitoring the mobility status of sample.In addition, in the position of corresponding bottom plate ridge
It puts, the upward beam of upper cover plate, can be inserted among the camera bellows of light sensing detector.
The optical signal sent out from the lightening hole of kit using the acquisition of light sensing detector, is converted into electric signal, according to
Standard curve obtains the residual quantity of pesticide veterinary drug in sample.
The light sensing detector external form is in cuboid, specification 130mm × 75mm × 30mm (Fig. 3), including photoelectric sensing
The components such as device, micro current amplifier, voltage follower and electronic switch, microcontroller and display.Class on the market can also be used
As light sensing detector product for detecting.
The present invention further provides the chemiluminescence immunoassay detection reagent item or the kit in detection pesticide veterinary drug
Application in residual.
During measure, with the enzyme adsorbed on the sample to be tested being added dropwise in sample pad and the glass fibre membrane or polyamides ester film
The detected material of label is as mobile phase, by detected material antibody fixed on the nitrocellulose filter, luminous agent film and mistake
The luminous zone of urea film composition is aoxidized, as stationary phase, luminous zone institute's luminous intensity is with the content of determinand in sample in corresponding
Proportionate relationship by light sensing detector, directly displays measurement result.
The present invention is using chemiluminescence immunoassay detection reagent item, based on antigen-antibody reaction combination chemiluminescence, as letter
Number source, measurement result is directly displayed by light sensing detector, and this method is quick, simple and direct, accurate, for quantitative detection food middle peasant
Medicine and residue of veterinary drug provide effective measures.It is signal source with direct light, rather than the colour band ocular estimate of colloid gold reagent item.This
Method cannot be only used for the detection of pesticide, residue of veterinary drug, can also be widely used in clinical medicine drug, hormone etc., environment prison
The multinomial detection such as a variety of toxin, Pollution by Chemicals in survey.
Description of the drawings
Fig. 1 is the structure diagram that the present invention is used to detect the chemiluminescence immunoassay detection reagent item of pesticide residue of veterinary drug；
Wherein, 1- sample pads, 2- is adsorbed with the glass fibre membrane of the detected material of enzyme label or polyamides ester film, 3- are fixed with detected material
The nitrocellulose filter of antibody is sprayed with antibody (middle setting-out position), 4- luminous agent films, 5- urea peroxide films, 6- absorption pads, 7-
PVC liner plates.
Fig. 2 is the vertical view that the present invention is used to detect the chemiluminescence immune detection reagent kit of pesticide residue of veterinary drug；Wherein,
8- lightening holes, 9- observation windows, 10- wells, 11- central baffles and middle ridge.
Fig. 3 is the front view of light sensing detector of the present invention；Wherein, 12- displays, 13- camera bellows, 14- switches, 15- schools
Positive key, 16- measure key.
Fig. 4 is the chloramphenicol standard curve drawn in the embodiment of the present invention 2.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
The percentage sign " % " arrived involved in the present invention, if not specified, refers to mass percent；But the percentage of solution
Than unless otherwise specified, referring to the grams containing solute in 100mL solution.
In the present invention, if not specified, the orientation or state relation of the instructions such as term " on ", " under " are for only for ease of
The description present invention and simplified description rather than instruction imply that signified device or element must be with specific orientation, Yi Te
Fixed azimuth configuration and operation, therefore be not considered as limiting the invention.
Embodiment 1 is used to detect the chemiluminescence immunoassay detection reagent item of residual chloromycetin
1st, the preparation of chloramphenicol antibody
(it is immunogene with chloramphenicol crosslinking bovine serum albumin(BSA), new zealand rabbit is immunized using mostly anti-in the present embodiment
Gained immune serum), potency 1:320000, purified rear compound concentration is 3mg/ml, and available antibodies dilution presses 1:20-300 times
Than dilution, used in the present embodiment by 1:Antibody liquid, is sprayed on nitrocellulose filter (NC) by 35 dilutions with Membrane jetter；Then by film
In drying at room temperature 30 minutes, the drying 2 hours of 37 DEG C of vacuum was to get being fixed with the NC films of chloramphenicol antibody.
2nd, the preparation of chloramphenicol-HRP
Flow is as follows：
A concentration of 0.3mg/ml of CAP-HRP by the use of 0.1M PBS liquid (pH8.0) as crosslinking agent dilution when using, can be pressed
1:30-50 dilutes.1 is pressed in the present embodiment:30 dilutions, with this dilution sized glass fibres film 30 minutes, then drying at room temperature
30 minutes, the drying 2 hours of 37 DEG C of vacuum was to get CAP-HRP films.
3rd, the preparation of luminol film (luminous agent film)
Luminol is dissolved in 0.5M sodium carbonate-bicarbonates buffer solution (pH8.9), 0.5-10mM solution is made into, adds
Add 0.1% pair of iodophenol.The glass fibre membrane item 15 minutes for being 3mm with this liquid infiltration width, then drying at room temperature 30 minutes, vacuum
37 DEG C of dryings 2 hours are to get luminol film item.
4th, the preparation of urea peroxide film
With 0.1M Tris-HCL (pH8.5) buffer solution, appropriate diformazan its sulfoxide is added as cosolvent, is made into concentration
For 20% urea peroxide liquid, the polyester film item that infiltration width is 3mm 10 minutes, then drying at room temperature 30 minutes, 37 DEG C of dryings 2 of vacuum
Hour is to get urea peroxide film item.
5th, the assembling of reagent strip
With the PVC liner plates of wide 5cm, urea peroxide film item, the NC films, the CAP-HPP that are fixed with chloramphenicol antibody are sticked step by step
Film, sample pad film item and absorption pad film item, correspond at urea peroxide film item on NC films, luminol film are fixed with adhesive tape
Full wafer is cut into the reagent strip of 4mm wide with cutting machine by item.The chemiluminescence immunoassay for being used to detect residual chloromycetin of the present embodiment
Detection reagent length and width are 50mm*4mm.
Wherein, the long 8-10mm of the sample pad, material are selected from polyamides ester, glass fibre or filter paper；The CAP-HPP films are long
3-5mm, the long 20-30mm of NC films for being fixed with chloramphenicol antibody；The antibody is fixed on it close to sample pad side NC films
At 6-10mm；The long 2-5mm of luminol film, material are selected from polyamides ester, glass fibre or filter paper；The long 2- of urea peroxide film
5mm, material are selected from polyamides ester, glass fibre or filter paper；The long 12-15mm of absorption pad, material is filter paper.
The sample pad, CAP-HPP films, the NC films for being fixed with chloramphenicol antibody, luminol film and absorption pad interact successively
Stacking is pasted on PVC liner plates；The sample pad is pressed in 1-2mm on the CAP-HPP films, and the CAP-HPP membrane pressures are described
3-4mm on the NC films of chloramphenicol antibody is fixed with, the NC films other end for being fixed with chloramphenicol antibody is at edge 5-7mm
The luminol film is fixed with adhesive tape, and the absorption pad, which is pressed in, described is fixed with 2-3mm on the NC films of chloramphenicol antibody.Its
In, the urea peroxide film is arranged on the lower section of the NC films for being fixed with chloramphenicol antibody, the position with the luminol film
It is corresponding.
6th, the assembling of contrast agents item
Step is only the NC film items with unlocked antibody, i.e. blank NC films with above-mentioned chemiluminescence immunoassay detection reagent item
Item replaces being fixed with the NC film items of antibody.
It is as shown in Figure 1 for detecting the chemiluminescence immunoassay detection reagent structure of residual chloromycetin after assembling.
Embodiment 2 is used to detect chemiluminescence immune detection reagent kit and the application of residual chloromycetin
The detection kit of the present embodiment includes box body and cover board, parallel on the bottom plate of the box body to be equipped with two size phases
With groove, the chemiluminescence immunoassay detection reagent item and contrast agents item of the preparation of embodiment 1 are placed in groove respectively；Between two slots
There is ridge to separate it, when covering cover board, tray interior is divided into two lighttight spaces by central baffle and middle ridge.
Described cover board one end is equipped at least one well, corresponding to the sample pad of the reagent strip；The cover board is another
The rapid parallel position for being equipped with two lightening hole, corresponding respectively to luminous agent film on the reagent strip.It can be in the well and hair
At least one observation window is equipped between unthreaded hole, for monitoring the mobility status of sample.In addition, in the position of corresponding bottom plate ridge, on
The upward beam of cover board can be inserted among the camera bellows of light sensing detector.
1st, the drafting of chloramphenicol standard curve
1. light sensing detector is transferred to correction shelves.
2. to the well of kit, it is separately added into chloramphenicol titer:0.05、0.1、0.3、0.5、1.0、2.0、
4.0ng/ml is 200 μ l per hole sample-adding amount.
3. after sample-adding, when observation fluid sample reaches observation window, pulling out the camera bellows of detector, kit being put into, promote dark
Case is returned in detector, after 3 minutes, starts to measure.Display shows control wells (corresponding to contrast agents item) with measuring hole
(corresponding to chemiluminescence immunoassay detection reagent item) the ratio between ratio, i.e., the numerical value being converted by electric signal makes standard song with this
Line.As a result as shown in table 1 and Fig. 4.
1 control wells of table are with measuring boring ratio value
Note：Ratio is the percentage for obtaining ratio using each concentration readings as molecule respectively using control wells numerical value as denominator
Number using ratio as ordinate, maps by abscissa of the logarithm of concentration, obtains standard curve.
By this standard curve and formula：Y=0.174ln (x)+0.603, R2=0.989, it is pre-stored in the light sensing detection
In the microcontroller of instrument, that is, complete the correction of detector.
During Site Detection, detector is transferred to measure shelves, by above operation, adds in 200 μ l sample liquids in well every time, will
Reagent barrel is put into camera bellows after 3 minutes, is read once at interval of 5 seconds instruments, and each sample reads 5 numerical value, and it is average to calculate it
It is worth for measured value, by being compareed with the standard curve value to prestore, you can immediately arrive at result from display screen.Unitary determination only needs
5 minutes.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (4)
1. for detecting the chemiluminescence immunoassay detection reagent item of residual chloromycetin, which is characterized in that including sample pad (1), inhale
With the enzyme label glass fibre membrane (2) of chloramphenicol, the NC films (3) for being fixed with chloramphenicol antibody, luminous agent film (4), peroxidating
Urea film (5), absorption pad (6) and PVC liner plates (7)；
The sample pad (1), the glass fibre membrane (2) for being adsorbed with enzyme label chloramphenicol, the NC films for being fixed with chloramphenicol antibody
(3), luminous agent film (4) and absorption pad (6) are pasted onto successively on PVC liner plates (7)；Wherein, the urea peroxide film (5) is arranged on
The lower section of NC films (3) for being fixed with chloramphenicol antibody, it is corresponding with the position of the luminous agent film (4)；
The preparation of S1, chloramphenicol antibody
It is immunogene with chloramphenicol crosslinking bovine serum albumin(BSA), immune serum conduct obtained by new zealand rabbit is immunized and resists more, effect
Valency 1:320000, purified rear compound concentration is 3mg/ml, and 1 is pressed with antibody diluent:20-300 doubling dilutions, will be anti-with Membrane jetter
Body fluid is sprayed on NC films；Then by film in drying at room temperature 30 minutes, the drying 2 hours of 37 DEG C of vacuum resists to get being fixed with chloramphenicol
The NC films of body；
The preparation of S2, CAP-HRP film
Flow is as follows：
A concentration of 0.3mg/ml of CAP-HRP, by the use of pH8.0 0.1M PBS liquid as crosslinking agent dilution when using, by 1:30-50
Dilution；With this dilution sized glass fibres film 30 minutes, then drying at room temperature 30 minutes, the drying 2 hours of 37 DEG C of vacuum obtained
CAP-HRP films are as adsorbed with the glass fibre membrane of enzyme label chloramphenicol；
The preparation of S3, luminol film
Luminol is dissolved in pH8.9 0.5M sodium carbonate-bicarbonate buffer solutions, is made into 0.5-10mM solution, is added
0.1% pair of iodophenol；The glass fibre membrane item 15 minutes for being 3mm with this liquid infiltration width, then drying at room temperature 30 minutes, vacuum 37
DEG C dry 2 hours to get luminol film, as luminous agent film；
The preparation of S4, urea peroxide film
With pH8.5 0.1M Tris-HCL buffer solutions, its sulfoxide of appropriate diformazan is added to be made into a concentration of 20% mistake as cosolvent
Aoxidize urea liquid, the polyester film item 10 minutes that infiltration width is 3mm, then drying at room temperature 30 minutes, the drying 2 hours of 37 DEG C of vacuum, i.e.,
Obtain urea peroxide film；
The assembling of S5, reagent strip
With the PVC liner plates of wide 5cm, urea peroxide film, the NC films for being fixed with chloramphenicol antibody, CAP-HRP films, sample are sticked step by step
Pad and absorption pad, correspond at urea peroxide film on NC films, luminol film are fixed with adhesive tape, be cut into full wafer with cutting machine
The reagent strip of 4mm wide；It is 50mm*4mm wherein for detecting the chemiluminescence immunoassay detection reagent length and width of residual chloromycetin；
Wherein, the long 8-10mm of the sample pad, material are selected from polyamides ester, glass fibre or filter paper；The long 3- of CAP-HRP films
5mm, the long 20-30mm of NC films for being fixed with chloramphenicol antibody；The antibody is fixed on it close to sample pad side NC films 6-
At 10mm；The long 2-5mm of luminol film, material are selected from polyamides ester, glass fibre or filter paper；The long 2- of urea peroxide film
5mm, material are selected from polyamides ester, glass fibre or filter paper；The long 12-15mm of absorption pad, material is filter paper；
The sample pad, CAP-HRP films, the NC films for being fixed with chloramphenicol antibody, luminol film and absorption pad interaction cascading successively
It pastes on PVC liner plates；The sample pad is pressed in 1-2mm on the CAP-HRP films, and the CAP-HRP membrane pressures are in the fixation
There is 3-4mm on the NC films of chloramphenicol antibody, the NC films other end for being fixed with chloramphenicol antibody is at edge 5-7mm with saturating
Gelatin band fixes the luminol film, and the absorption pad, which is pressed in, described is fixed with 2-3mm on the NC films of chloramphenicol antibody；Wherein,
The urea peroxide film is arranged on the lower section of the NC films for being fixed with chloramphenicol antibody, opposite with the position of the luminol film
It should.
2. the chemiluminescence immune detection reagent kit for being used to detect residual chloromycetin containing reagent strip described in claim 1,
Be characterized in that, by the reagent strip be placed in a plastics get stuck it is middle assembling obtain；
It is described to get stuck including box body and cover board, it is parallel on the bottom plate of the box body to be equipped with two identical grooves of size, in groove
The chemiluminescence immunoassay detection reagent item and contrast agents item are placed respectively；There is ridge to separate it between two slots, when covering cover board,
Tray interior is separated into two lighttight spaces；Wherein, the contrast agents item is to detect the chemiluminescence immunoassay
The NC films for being fixed with chloramphenicol antibody on reagent strip are substituted for what blank nitrocellulose filter obtained；
Described cover board one end is equipped at least one well, corresponding to the sample pad of the reagent strip；The cover board is another rapid flat
Row is equipped with two lightening holes, corresponds respectively to the position of luminous agent film on the reagent strip；Optionally in the well and shine
At least one observation window is equipped between hole, for monitoring the mobility status of sample.
3. application of the kit described in reagent strip described in claim 1 or claim 2 in residual chloromycetin is detected.
4. application according to claim 3, which is characterized in that during measure, with the sample to be tested that is added dropwise in sample pad and
The enzyme label chloramphenicol adsorbed on the glass fibre membrane is as mobile phase, by fixed chloramphenicol antibody, hair on the NC films
The luminous zone of photo etching film and urea peroxide film composition, as stationary phase, luminous zone institute's luminous intensity contains with determinand in sample
Amount, by light sensing detector, directly displays measurement result in corresponding proportionate relationship.
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| CN101473216A (en) * | 2006-05-09 | 2009-07-01 | 贝克曼考尔特公司 | Nonseparation assay methods |
| CN104849264A (en) * | 2015-06-08 | 2015-08-19 | 中国科学院电子学研究所 | Chemiluminiscence immune test strip and preparation method thereof |
| CN105675873A (en) * | 2016-01-04 | 2016-06-15 | 洛阳普莱柯万泰生物技术有限公司 | Detection kit and application thereof |
| CN105891491A (en) * | 2016-04-11 | 2016-08-24 | 洛阳普莱柯万泰生物技术有限公司 | Kit and application thereof |
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