CN106568939A - Chemiluminesent immunoassay reagent strip, and application thereof in detection of pesticide and veterinary drug residual - Google Patents
Chemiluminesent immunoassay reagent strip, and application thereof in detection of pesticide and veterinary drug residual Download PDFInfo
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- CN106568939A CN106568939A CN201610933988.6A CN201610933988A CN106568939A CN 106568939 A CN106568939 A CN 106568939A CN 201610933988 A CN201610933988 A CN 201610933988A CN 106568939 A CN106568939 A CN 106568939A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
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Abstract
The invention provides a chemiluminesent immunoassay reagent strip for detecting the pesticide and veterinary drug residual. The reagent strip comprises a sample pad (1), a glass fiber film or polyamideester film (2) adsorbed with an enzyme-labeled detected substance, a cellulose nitrate film (3) fixed with a detected substance antibody, a luminescence reagent film (4), a urea peroxide film (5) and an absorbent pad (6), and all above parts are sequentially pasted on a PVC lining plate (7). In the detection process, a sample-to-be-detected which is dropwise added to the sample pad and the enzyme-labeled detected substance adsorbed by the glass fiber film or polyamideester film are adopted as a mobile phase the detected substance antibody fixed on the cellulose nitrate film, the luminescence reagent film and the urea peroxide film are adopted as a stationary phase, a corresponding proportion relationship exists between the luminescence intensity in a luminescence area and the content of the detected substance in the sample, and a detection result is directly displayed through a light sensing detector. The reagent strip provides a rapid, simple and accurate detection means for quantitative detection of the pesticide and veterinary drug residual in a food.
Description
Technical field
The invention belongs to the technical field of biological in biomedicine field, specifically, is related to a kind of chemiluminescence
The application of immunologic function test reagent bar and its detection pesticide residue of veterinary drug.
Background technology
Current detection technique mainly includes two developing direction, the inspection technology side that the first is automatically controlled to large-scale precision
Face is developed, and makes inspection technology standardization, precision, intensive degree more and more higher, its be second to economic, quick, universalness,
Popular detection technique aspect development, to meet the demand of popular self health care, self-protection consciousness.With regard to food safety
Speech, the supervision project of national regulation, pesticide has 136 kinds, and veterinary drug has 217 kinds.In the face of so many detection species, inspection how is improved
Survey efficiency, quantitative and easy detection Pesticide and residue of veterinary drug, always one problem demanding prompt solution.
Currently for pesticide veterinary drug quick detection, using it is most be " colloidal gold immunochromatographimethod reagent strip ".The reagent strip
It using antibody and the sample liquid of colloid gold particle absorption is mobile phase to be, the determinand (antigen) in sample is with to be fixed on nitric acid fine
Antigen (fixing phase) on dimension film, common competition and antibodies, antigen is more in sample, many with antibodies in gold colloidal, then
It is deposited on that antibody in fixing phase is few, in the position of fixing phase, the red line of display is shallow.Conversely, determinand is few in sample, display
Red line depth.Judged with the red depth of perusal residue number, if it is exceeded.This method is easy, quickly.But tie
Fruit is judged according to range estimation, often equivocal.Colloid gold reagent bar can only be used for qualitative, positive-exceeded, feminine gender-not exceeded,
Using there is certain limitation.The test limit needed for detection can not be reached under certain situation.There is now " colloidal-gold strip chart scanner ", lead to
Cross and emit light on film, read reflected light intensity come determine fixing phase redness the depth, the amount of residue is quantitative determined with this.
But instrument is by refraction of the launching light on film, intensity of reflected light is detected, its sensitivity, accuracy have certain problem.
The content of the invention
It is an object of the invention to provide a kind of chemiluminescence immunoassay detectable bar and its detection pesticide residue of veterinary drug in
Application.
In order to realize the object of the invention, the present invention is used to detect the chemiluminescence immunoassay detectable of pesticide residue of veterinary drug
Bar, including sample pad 1, be adsorbed with enzyme labelling detected material (enzyme-labelled antigen) glass fibre membrane or polyamides ester film 2, be fixed with
Nitrocellulose filter (the NC films) 3, luminous agent film 4 of detected material antibody, urea peroxide film 5, absorption pad 6 and PVC liner plates 7.
The sample pad 1, be adsorbed with enzyme labelling detected material glass fibre membrane 2, be fixed with detected material antibody
Nitrocellulose filter 3, the luminous agent film 4 and absorption pad 6 are pasted onto successively on PVC liner plates 7；Wherein, the urea peroxide film 5
The lower section of the nitrocellulose filter 3 for being fixed with detected material antibody is arranged on, it is relative with the position of the luminous agent film 4
Should.Reagent strip structure is as shown in Figure 1.
The PVC liner plates long 40-60mm, wide 3-4mm；The long 8-10mm of the sample pad, wide 3-4mm, material is selected from polyamides
Ester, glass fibre or filter paper；The glass fibre membrane or the long 3-5mm of polyamides ester film of the detected material for being adsorbed with enzyme labelling, it is wide
3-4mm, the enzyme is horseradish peroxidase；The long 20-30mm of the nitrocellulose filter for being fixed with detected material antibody, it is wide
3-4mm, the antibody is monoclonal antibody or polyclonal antibody；The antibody is fixed on it near sample pad side cellulose nitrate
At plain film 6-10mm；The long 2-5mm of the luminous agent film, wide 3-4mm, material is selected from polyamides ester, glass fibre or filter paper, used
Photo etching is luminol or derivatives thereof；The long 2-5mm of the urea peroxide film, wide 3-4mm, material is selected from polyamides ester, glass fibre
Or filter paper, wherein urea peroxide can be replaced with peroxide such as perbenzoic acids；The absorption pad long 12-15mm, wide 3-
4mm, material is selected from filter paper material.
The sample pad is adsorbed with 1- on the glass fibre membrane of the detected material of enzyme labelling or polyamides ester film described in being pressed in
2mm, the glass fibre membrane or polyamides ester mould of the detected material for being adsorbed with enzyme labelling resist in the detected material that is fixed with
3-4mm on the nitrocellulose filter of body, the nitrocellulose filter other end for being fixed with detected material antibody is apart from edge 5-
Paste the luminous agent film at 7mm, the absorption pad is pressed in and described is fixed with 2- on the nitrocellulose filter of detected material antibody
3mm。
The glass fibre membrane or polyamides ester film of the detected material for being adsorbed with enzyme labelling is that enzyme labelling detected material is molten
Liquid is sprayed at what Jing after glass fibre membrane or polyamides ester film was dried to obtain；
The nitrocellulose filter for being fixed with detected material antibody is that detected material antibody-solutions are sprayed at into nitric acid fibre
Jing is dried to obtain after the plain film of dimension.
It by concentration is 0.5-10.0mM luminols or derivatives thereof solution spraying drying on film that the luminous agent film is
Obtain；Wherein, the sodium carbonate-bicarbonate buffering of described luminol or derivatives thereof solution 0.1-1%, pH8-9.5 is molten
Liquid is prepared；
The urea peroxide film is soaked after film with the urea peroxide solution that concentration is 10%-30%, what drying was obtained.
The detected material includes various antibiotic, steroid hormone and pesticide.Preferably, the detected material includes chlorine
Mycin, gentamycin, progesterone, testosterone, chloramines are clever, many prodigiosins, and can obtain each type organic of associated antibodies etc..
The present invention also provides the change for detecting pesticide residue of veterinary drug containing the chemiluminescence immunoassay detectable bar
Learn electrochemiluminescent immunoassay detection kit, its by the reagent strip be placed in a plastics get stuck it is middle assembling obtain.
It is described to get stuck including box body and cover plate, it is parallel on the base plate of the box body to be provided with two size identical grooves, it is recessed
The chemiluminescence immunoassay detectable bar and contrast agents bar are placed respectively in groove；There is ridge to separate it between two grooves, close the lid
During plate, tray interior is separated into two lighttight spaces.Reagent cartridge configuration is as shown in Figure 2.
Wherein, the contrast agents bar is to resist the detected material that is fixed with the chemiluminescence immunoassay detectable bar
The nitrocellulose filter of body is substituted for what blank nitrocellulose filter was obtained.
Described cover plate one end is provided with least one well, corresponding to the sample pad of the reagent strip；The cover plate is another
Rapids is parallel to be provided with two lightening holes, corresponds respectively to the position of luminous agent film on the reagent strip.Optionally can be in the well
At least one observation window is provided with and lightening hole between, for monitoring the mobility status of sample.Additionally, in the position of correspondence base plate ridge
Put, a upper cover plate upwardly beam, among can be inserted into the camera bellows of light sensing detector.
The optical signal sent from the lightening hole of test kit using the collection of light sensing detector, is converted into the signal of telecommunication, according to
Standard curve, draws the residual quantity of sample Pesticides veterinary drug.
The light sensing detector external form is in cuboid, specification 130mm × 75mm × 30mm (Fig. 3), including photoelectric sensing
The component such as device, micro current amplifier, voltage follower and electrical switch, single-chip microcomputer and display.Can also be using class on the market
As light sensing detector product be used for detect.
The present invention further provides the chemiluminescence immunoassay detectable bar or the test kit are in detection pesticide veterinary drug
Application in residual.
During measure, with the enzyme adsorbed on the testing sample of Deca in sample pad and the glass fibre membrane or polyamides ester film
The detected material of labelling as mobile phase, by detected material antibody fixed on the nitrocellulose filter, luminous agent film and mistake
The luminous zone of oxidation urea film composition, used as fixing phase, luminous zone institute's luminous intensity is with the content of determinand in sample in corresponding
Proportionate relationship, by light sensing detector, directly displays measurement result.
The present invention utilizes chemiluminescence immunoassay detectable bar, chemiluminescence is combined based on antigen antibody reaction, as letter
Number source, by light sensing detector measurement result is directly displayed, and the method is quick, simple and direct, accurate, is detection by quantitative food middle peasant
Medicine and residue of veterinary drug provide effective measures.It is signal source with direct light, and unlike the colour band ocular estimate of colloid gold reagent bar.This
Method cannot be only used for the detection of pesticide, residue of veterinary drug, can also be widely used in clinical medicine medicine, hormone etc., environment prison
The multinomial detection such as various toxin, Pollution by Chemicals in survey.
Description of the drawings
Fig. 1 is the present invention for detecting the structural representation of the chemiluminescence immunoassay detectable bar of pesticide residue of veterinary drug；
Wherein, 1- sample pad, 2- is adsorbed with the glass fibre membrane of the detected material of enzyme labelling or polyamides ester film, and 3- is fixed with detected material
The nitrocellulose filter of antibody, is sprayed with antibody (middle setting-out position), 4- luminous agent films, 5- urea peroxide films, 6- absorption pads, 7-
PVC liner plates.
Fig. 2 is the present invention for detecting the top view of the chemiluminescence immune detection reagent kit of pesticide residue of veterinary drug；Wherein,
8- lightening holes, 9- observation windows, 10- wells, 11- central baffles and middle ridge.
Fig. 3 is the front view of light sensing detector of the present invention；Wherein, 12- display, 13- camera bellows, 14- switches, 15- schools
Positive key, 16- determines key.
Fig. 4 is the chloromycetin standard curve drawn in the embodiment of the present invention 2.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional meanses that are well known to those skilled in the art of technological means used, it is raw materials used to be commercial goods.
The percentage sign " % " being related in the present invention, if not specified, refers to mass percent；But the percentage of solution
Than unless otherwise specified, referring to the grams containing solute in 100mL solution.
In the present invention, if not specified, term " on ", the orientation of the instruction such as D score or state relation be for only for ease of
The description present invention and simplified description, rather than indicate or imply that the device or element of indication must have specific orientation, Yi Te
Fixed azimuth configuration and operation, therefore be not considered as limiting the invention.
Embodiment 1 is used to detect the chemiluminescence immunoassay detectable bar of residual chloromycetin
1st, the preparation of chloramphenicol antibody
(it is immunogen to be crosslinked bovine serum albumin with chloromycetin to multi-resistance, immune new zealand rabbit used in the present embodiment
Gained immune serum), potency 1:320000, purified rear compound concentration is 3mg/ml, and available antibodies diluent presses 1:20-300 times
Than dilution, with by 1 in the present embodiment:35 dilutions, are sprayed on antibody liquid on nitrocellulose filter (NC) with Membrane jetter；Then by film
In drying at room temperature 30 minutes, the drying 2 hours of 37 DEG C of vacuum obtained final product the NC films for being fixed with chloramphenicol antibody.
2nd, the preparation of chloromycetin-HRP
Flow process is as follows：
CAP-HRP concentration is 0.3mg/ml, can be pressed as cross-linking agent diluent with 0.1M PBS liquid (pH8.0) when using
1:30-50 dilutes.1 is pressed in the present embodiment:30 dilutions, with this diluent sized glass fibres film 30 minutes, then drying at room temperature
30 minutes, the drying 2 hours of 37 DEG C of vacuum obtained final product CAP-HRP films.
3rd, the preparation of luminol film (luminous agent film)
Luminol is dissolved in 0.5M sodium carbonate-bicarbonate buffer solution (pH8.9), 0.5-10mM solution is made into, is added
Plus 0.1% pair of iodophenol.Moisten the glass fibre membrane bar 15 minutes of a width of 3mm with this immersion, then drying at room temperature 30 minutes, vacuum
37 DEG C of dryings 2 hours, obtain final product luminol film bar.
4th, the preparation of urea peroxide film
With 0.1M Tris-HCL (pH8.5) buffer solution, add appropriate its sulfoxide of diformazan as cosolvent, be made into concentration
For 20% urea peroxide liquid, the polyester film bar of a width of 3mm is infiltrated 10 minutes, then drying at room temperature 30 minutes, 37 DEG C of dryings 2 of vacuum
Hour, obtain final product urea peroxide film bar.
5th, the assembling of reagent strip
With the PVC liner plates of wide 5cm, urea peroxide film bar is sticked step by step, NC films, the CAP-HPP of chloramphenicol antibody is fixed with
Film, sample pad film bar and absorption pad film bar, at correspondence urea peroxide film bar on NC films, with adhesive tape luminol film are fixed
Bar, the wide reagent strips of 4mm are cut into cutting machine by full wafer.The chemiluminescence immunoassay for detecting residual chloromycetin of the present embodiment
The a width of 50mm*4mm of detectable bar length.
Wherein, the long 8-10mm of the sample pad, material is selected from polyamides ester, glass fibre or filter paper；The CAP-HPP films are long
3-5mm, the long 20-30mm of NC films for being fixed with chloramphenicol antibody；The antibody is fixed on it near sample pad side NC films
At 6-10mm；The long 2-5mm of the luminol film, material is selected from polyamides ester, glass fibre or filter paper；The long 2- of the urea peroxide film
5mm, material is selected from polyamides ester, glass fibre or filter paper；The long 12-15mm of the absorption pad, material is filter paper.
The sample pad, CAP-HPP films, the NC films for being fixed with chloramphenicol antibody, luminol film and absorption pad are interacted successively
Stacking is pasted on PVC liner plates；The sample pad is pressed in 1-2mm on the CAP-HPP films, and the CAP-HPP moulds are described
3-4mm on the NC films of chloramphenicol antibody is fixed with, the NC films other end for being fixed with chloramphenicol antibody is at edge 5-7mm
The luminol film is fixed with adhesive tape, the absorption pad is pressed in and described is fixed with 2-3mm on the NC films of chloramphenicol antibody.Its
In, the urea peroxide film is arranged on the lower section of the NC films for being fixed with chloramphenicol antibody, the position with the luminol film
It is corresponding.
6th, the assembling of contrast agents bar
Step, with above-mentioned chemiluminescence immunoassay detectable bar, is only with the NC film bars of unlocked antibody, i.e. blank NC films
Bar replaces being fixed with the NC film bars of antibody.
The chemiluminescence immunoassay detectable bar structure for detecting residual chloromycetin after assembling is as shown in Figure 1.
Embodiment 2 is used to detect chemiluminescence immune detection reagent kit and the application of residual chloromycetin
The detection kit of the present embodiment includes box body and cover plate, parallel on the base plate of the box body to be provided with two size phases
Place chemiluminescence immunoassay detectable bar and contrast agents bar prepared by embodiment 1 in same groove, groove respectively；Between two grooves
There is ridge to separate it, when covering cover plate, tray interior is divided into two lighttight spaces by central baffle and middle ridge.
Described cover plate one end is provided with least one well, corresponding to the sample pad of the reagent strip；The cover plate is another
Rapids is parallel to be provided with two lightening holes., correspond respectively to the position of luminous agent film on the reagent strip.In the well and can send out
At least one observation window is provided between unthreaded hole, for monitoring the mobility status of sample.Additionally, in the position of correspondence base plate ridge, on
A cover plate upwardly beam, among can be inserted into the camera bellows of light sensing detector.
1st, the drafting of chloromycetin standard curve
1. light sensing detector is transferred to into correction shelves.
2. to the well of test kit, it is separately added into chloromycetin titer:0.05、0.1、0.3、0.5、1.0、2.0、
4.0ng/ml, is 200 μ l per hole sample-adding amount.
3. after being loaded, when observation fluid sample reaches observation window, the camera bellows of detector is pulled out, test kit is put into, propulsion is dark
Case is returned in detector, after 3 minutes, starts to determine.Display shows control wells (corresponding to contrast agents bar) and determines hole
(corresponding to chemiluminescence immunoassay detectable bar) ratio, i.e., the ratio of the numerical value being converted into by the signal of telecommunication makes standard bent with this
Line.As a result as shown in table 1 and Fig. 4.
The control wells of table 1 and measure boring ratio value
Note：Ratio is, with control wells numerical value as denominator, respectively the percentage of ratio to be drawn as molecule with each concentration readings
Number, with ratio as vertical coordinate, maps by abscissa of the logarithm of concentration, obtains standard curve.
By this standard curve and formula：Y=0.174ln (x)+0.603, R2=0.989, it is pre-stored in the light sensing detection
In the single-chip microcomputer of instrument, that is, complete the correction of detector.
During Site Detection, detector is transferred to measure shelves, operates by more than, adds 200 μ l sample liquid in well every time, will
Reagent barrel is put in camera bellows after 3 minutes, is read once at interval of 5 seconds instruments, and each sample reads 5 numerical value, calculates its average
It is worth for measured value, by compareing with the standard curve value for prestoring, you can immediately arrive at result from display screen.Unitary determination only needs
5 minutes.
Although above with a general description of the specific embodiments the present invention is described in detail,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, without departing from theon the basis of the spirit of the present invention these modifications or improvements, belong to the scope of protection of present invention.
Claims (9)
1. be used to detect the chemiluminescence immunoassay detectable bar of pesticide residue of veterinary drug, it is characterised in that including sample pad (1),
The glass fibre membrane or polyamides ester film (2) that are adsorbed with the detected material of enzyme labelling are, the cellulose nitrate for being fixed with detected material antibody
Plain film (3), luminous agent film (4), urea peroxide film (5), absorption pad (6) and PVC liner plates (7)；
The sample pad (1), be adsorbed with enzyme labelling detected material glass fibre membrane (2), be fixed with detected material antibody
Nitrocellulose filter (3), luminous agent film (4) and absorption pad (6) are pasted onto successively on PVC liner plates (7)；Wherein, the peroxidating
Urea film (5) is arranged on the lower section of the nitrocellulose filter (3) for being fixed with detected material antibody, with the luminous agent film (4)
Position it is corresponding.
2. reagent strip according to claim 1, it is characterised in that the PVC liner plates long 40-60mm, wide 3-4mm；It is described
The long 8-10mm of sample pad, wide 3-4mm, material is selected from polyamides ester, glass fibre or filter paper；It is described to be adsorbed with being detected for enzyme labelling
The glass fibre membrane or the long 3-5mm of polyamides ester film of thing, wide 3-4mm, the enzyme is horseradish peroxidase；It is described be fixed with it is tested
The long 20-30mm of nitrocellulose filter of thing antibody, wide 3-4mm are surveyed, the antibody is monoclonal antibody or polyclonal antibody；It is described
Antibody is fixed on it at the nitrocellulose filter 6-10mm of sample pad side；The long 2-5mm of the luminous agent film, wide 3-4mm, material
Matter is selected from polyamides ester, glass fibre or filter paper, and luminous agent used is luminol or derivatives thereof；The long 2- of the urea peroxide film
5mm, wide 3-4mm, material is selected from polyamides ester, glass fibre or filter paper；The long 12-15mm of the absorption pad, wide 3-4mm, material choosing
From filter paper material.
3. reagent strip according to claim 1 and 2, it is characterised in that the sample pad is pressed in and described is adsorbed with enzyme labelling
Detected material glass fibre membrane or polyamides ester film on 1-2mm, the glass fibre of the detected material for being adsorbed with enzyme labelling
Film or polyamides ester mould are fixed with 3-4mm on the nitrocellulose filter of detected material antibody described, it is described be fixed with it is detected
The nitrocellulose filter other end of thing antibody pastes the luminous agent film at edge 5-7mm, and the absorption pad is pressed in described
It is fixed with 2-3mm on the nitrocellulose filter of detected material antibody.
4. the reagent strip according to any one of claim 1-3, it is characterised in that the detected material for being adsorbed with enzyme labelling
Glass fibre membrane or polyamides ester film be that Jing does after glass fibre membrane or polyamides ester film by enzyme labelling detected material solution spraying
It is dry to obtain；
The nitrocellulose filter for being fixed with detected material antibody is that detected material antibody-solutions are sprayed at into celluloid
Jing is dried to obtain after film.
5. the reagent strip according to any one of claim 1-4, it is characterised in that it by concentration is 0.5- that the luminous agent film is
10.0mM luminols or derivatives thereof solution spraying drying on film is obtained；Wherein, described luminol or derivatives thereof is molten
The sodium carbonate-bicarbonate buffer preparation of liquid 0.1-1%, pH8-9.5；
The urea peroxide film is soaked after film with the urea peroxide solution that concentration is 10%-30%, what drying was obtained.
6. the reagent strip according to any one of claim 1-5, it is characterised in that the detected material includes various antibiosis
Element, steroid hormone and pesticide；Preferably, the detected material include chloromycetin, gentamycin, progesterone, testosterone, chloramines spirit,
Many prodigiosins and each type organic of associated antibodies can be obtained.
7. the chemiluminescence immunoassay for detecting pesticide residue of veterinary drug containing reagent strip described in any one of claim 1-6 is detected
Test kit, it is characterised in that its by the reagent strip be placed in a plastics get stuck it is middle assembling obtain；
It is described to get stuck including box body and cover plate, it is parallel on the base plate of the box body to be provided with two size identical grooves, in groove
The chemiluminescence immunoassay detectable bar and contrast agents bar are placed respectively；There is ridge to separate it between two grooves, when covering cover plate,
Tray interior is separated into two lighttight spaces；Wherein, the contrast agents bar is by chemiluminescence immunoassay detection
The nitrocellulose filter for being fixed with detected material antibody on reagent strip is substituted for what blank nitrocellulose filter was obtained；
Described cover plate one end is provided with least one well, corresponding to the sample pad of the reagent strip；The cover plate is another rapid flat
Row is provided with two lightening holes, corresponds respectively to the position of luminous agent film on the reagent strip；It is optional in the well and luminous
At least one observation window is provided between hole, for monitoring the mobility status of sample.
8. reagent strip described in any one of claim 1-6 or test kit described in claim 7 are in detection pesticide residue of veterinary drug
Using.
9. application according to claim 8, it is characterised in that during measure, with the testing sample of Deca in sample pad and
On the glass fibre membrane or polyamides ester film adsorb enzyme labelling detected material as mobile phase, by the nitrocellulose filter
The luminous zone of the detected material antibody, luminous agent film and urea peroxide film composition of upper fixation, used as fixing phase, luminous zone lights
Intensity is in corresponding proportionate relationship with the content of determinand in sample, by light sensing detector, directly displays measurement result.
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| CN201610933988.6A CN106568939B (en) | 2016-10-31 | 2016-10-31 | Chemiluminescence immunoassay detection reagent item and its application for detecting pesticide residue of veterinary drug |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146694A (en) * | 2019-04-26 | 2019-08-20 | 山东省食品药品检验研究院 | A kind of chloramphenicol direct competitive chemiluminescence high specific immunoassay method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997006439A1 (en) * | 1995-08-09 | 1997-02-20 | Quidel Corporation | Test strip and method for one step lateral flow assay |
| CN101473216A (en) * | 2006-05-09 | 2009-07-01 | 贝克曼考尔特公司 | Nonseparation assay methods |
| CN104849264A (en) * | 2015-06-08 | 2015-08-19 | 中国科学院电子学研究所 | Chemiluminiscence immune test strip and preparation method thereof |
| CN105675873A (en) * | 2016-01-04 | 2016-06-15 | 洛阳普莱柯万泰生物技术有限公司 | Detection kit and application thereof |
| CN105891491A (en) * | 2016-04-11 | 2016-08-24 | 洛阳普莱柯万泰生物技术有限公司 | Kit and application thereof |
-
2016
- 2016-10-31 CN CN201610933988.6A patent/CN106568939B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997006439A1 (en) * | 1995-08-09 | 1997-02-20 | Quidel Corporation | Test strip and method for one step lateral flow assay |
| CN101473216A (en) * | 2006-05-09 | 2009-07-01 | 贝克曼考尔特公司 | Nonseparation assay methods |
| CN104849264A (en) * | 2015-06-08 | 2015-08-19 | 中国科学院电子学研究所 | Chemiluminiscence immune test strip and preparation method thereof |
| CN105675873A (en) * | 2016-01-04 | 2016-06-15 | 洛阳普莱柯万泰生物技术有限公司 | Detection kit and application thereof |
| CN105891491A (en) * | 2016-04-11 | 2016-08-24 | 洛阳普莱柯万泰生物技术有限公司 | Kit and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146694A (en) * | 2019-04-26 | 2019-08-20 | 山东省食品药品检验研究院 | A kind of chloramphenicol direct competitive chemiluminescence high specific immunoassay method |
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