CN106561506A - Long-distance transportation method for euchiloglanis kishinouyei adult fishes - Google Patents

Long-distance transportation method for euchiloglanis kishinouyei adult fishes Download PDF

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Publication number
CN106561506A
CN106561506A CN201610993247.7A CN201610993247A CN106561506A CN 106561506 A CN106561506 A CN 106561506A CN 201610993247 A CN201610993247 A CN 201610993247A CN 106561506 A CN106561506 A CN 106561506A
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China
Prior art keywords
water
transportation
long
transport
euchiloglanis
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Pending
Application number
CN201610993247.7A
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Chinese (zh)
Inventor
魏开金
朱祥云
徐滨
甘维熊
邓龙君
马宝珊
徐进
刘小帅
曾如奎
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Yangtze River Fisheries Research Institute CAFS
Yalong River Hydropower Development Co Ltd
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Yangtze River Fisheries Research Institute CAFS
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Priority to CN201610993247.7A priority Critical patent/CN106561506A/en
Publication of CN106561506A publication Critical patent/CN106561506A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a long-distance transportation method for euchiloglanis kishinouyei adult fishes. The method comprises the steps: 1) adding a buffer liquid: adding a phosphate buffer liquid into transportation water and uniformly mixing the mixture; 2) putting fishes: putting the euchiloglanis kishinouyei adult fishes in an inflated bag holding transportation water; 3) performing gradient temperature reduction: after inflation, water-adding and oxygen filling of a plastic bag, performing gradient temperature reduction, reducing the temperature every 4 DEG C as a gradient, and reducing the water temperature to 2 DEG C; 4) performing encasement: putting the inflated bag which is reduced in temperature into a double-layered foam box with an interlayer filled with ice blocks, wherein the ice adding amount is adjusted according to the transportation time; 5) performing shipping and transportation: and 6) changing water: changing water with the buffer liquid every 12 hours in the transportation process and filling oxygen. The method disclosed by the invention is feasible and simple to operate. The survival rate of the euchiloglanis kishinouyei adult fishes after long-distance transportation is enhanced to a great extent, so that the survival rate of the euchiloglanis kishinouyei adult fishes after long-distance transportation is quite effectively improved, and a foundation for later species protection and related research work is laid.

Description

A kind of long-distance transport method of cordierite Pa Select-Committee adult fishes
Technical field
The invention belongs to technical field of aquaculture, is more particularly to a kind of long-distance transport method of cordierite Pa Select-Committee adult fishes.
Background technology
Cordierite Pa Select-Committee (Euchiloglanis david) are that Shi Pa Select-Committee belong to (Euchiloglanis Regan), SILURIFORMES (Siluriformes) , Select-Committee sections (Sisoridae) Fish, the category Fish is flat, and the big tail of head is little, and head is especially flat, dorsal fin Swell before starting point, body rear portion is flat-sided, delicious meat.The fish not yet has the report of artificial cultivation, therefore its yield is all relied on It is wild to fish for, cause its price into up-trend year by year.
The transportation of live fish method of Fish has important meaning in fish culture production process in the fishing etc. of wild parent fish Justice.Therefore Fish transportation survival rate is improved, it is indispensable in fish culture production to reduce the key issues such as cost of transportation Important step.
At present, because resource is day by day rare, the protective measure of this kind of Fish is gradually taken seriously, but because cordierite Pa Select-Committee stress Relatively strong, after long-distance transport, mortality rate is higher.
The content of the invention
The purpose of the present invention is to there are provided a kind of long-distance transport method of cordierite Pa Select-Committee adult fishes, easy to implement the method, operation Simplicity, the cordierite Pa Select-Committee adult fish survival rates through transporting for long-distance significantly are lifted, very effective to improve cordierite Pa Select-Committee adult fishes It is that the species conservation and correlational study work in later stage provides the foundation in the survival rate Jing after long-distance transport.
In order to realize above-mentioned purpose, the present invention adopts following technical measures:
A kind of long-distance transport method of cordierite Pa Select-Committee adult fishes, its step is:
1st, with buffer:By 6.78g disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), the hypophosphite monohydrates two of 0.17g bis- Hydrogen sodium (NaH2PO4·2H2O) dissolve, be settled to 100ml.As pH is 8.0 0.1M phosphate buffers (PBS).In every 1L The phosphate buffered solution of the 0.1M that 5ml pH are 8.0 is added in transport water, is mixed.
2nd, fish is put:Cordierite Pa Select-Committee adult fishes are put in the air bag (about 15-20 liters) equipped with transport water, traffic density is not More than 6/liter.
3rd, gradient cooling:Plastic bag inflation adds water after (volume of water exceedes the 2/3 of air bag capacity, is close to full) oxygenation, Gradient cooling is carried out, to be lowered the temperature as a gradient per 4 DEG C, each gradient continues 28-32 minutes, and most at last water temperature drops to 2 ℃。
4th, case:The air bag of cooling is put in two-ply foam case of the interlayer built with ice cube, amount on the rocks is according to transport Time adjusts, and 8-12 hours transport 4-6kg on the rocks;
5th, entrucking transport.
6th, water is changed:Transportation should change the water (this step 1) and oxygenation for adding buffer often more than 12 hours.
Described plastic bag is commercially available, and specification has but be not limited only to 30 ╳ 60cm, 40 ╳ 60cm;
Described oxygen is commercially available pure oxygen.
Long-distance transport:The long-distance transport of Fish refers to and for Fish to be transferred to other place by one place, and transport Distance is more than 200 kilometers or the time was more than 5 hours.
Studying for a long period of time through applicant, has invented a kind of long-distance transport mode of cordierite Pa Select-Committee adult fishes, through long-distance fortune After defeated, survival rate is obviously improved.
Cordierite Pa Select-Committee adult fishes Jing after this method long-distance transport, the adult fish with commonsense method transport exists in survival rate Huge difference, conventional method cannot be transported more than 10 hours, and Jing present invention transports, more than 20 hours, survival rate was more than 95%; And in later stage temporary rearing process, mortality rate is less than 3%.
The present invention compared with prior art, with advantages below and effect:
1. simple to operate, medicine is readily available:What the present invention adopted solution is all that commercially available Common drugs are prepared.
2., more than more than 20 hours, survival rate is high, and survival rate is more than 95% for haulage time.
3. stability is high:Complex operations are not related to, it is repeatable high.
Specific embodiment
Embodiment 1:
A kind of long-distance transport method of cordierite Pa Select-Committee adult fishes, its step is:(as a example by transport 500 tail cordierite Pa Select-Committee)
1. with buffer:The phosphate buffer (PBS) of the 0.1M for adding that 5ml pH are 8.0 in water is transported per 1L, is mixed, Prepare 500L transport water altogether.
2. fish is put:Cordierite is climbed into Select-Committee adult fishes to be put in the air bag equipped with transport water (air bag volume is about 20 liters), fortune / liter of defeated density 5,100 are less than per packed fish.
3. gradient cooling:Plastic bag inflation is added water to more than 2/3, about 15 liters, after oxygenation, gradient cooling is carried out, with per 4 DEG C Lowered the temperature for a gradient, each gradient continues 30 minutes, and most at last water temperature drops to 2 DEG C.
4. case:The air bag of cooling is put in two-ply foam case of the interlayer built with ice cube, amount on the rocks is according to transport Time adjusts, and this time haulage time is 20 hours, each two-ply foam case 8kg on the rocks.
5. entrucking transport, this time transport is 5 entruckings of morning.
6. water is changed:During at 5 in afternoon, more renew water, and add buffer, after checking ice cube, oxygenation continues to transport, in About 12 points of night reaches plants.
This time haulage time about 20 hours altogether, dead 5 tail adult fish, survival rate 99%, dead after temporarily supporting the phase (3 days) and terminating 2, mortality rate is 0.4%.

Claims (1)

1. a kind of long-distance transport method of cordierite Pa Select-Committee adult fishes, its step is:
1) with buffer:PH is that the compound method of the phosphate buffered solution of 8.0 0.1M is:By the hypophosphite monohydrates of 67.8g 12 Disodium hydrogen, the hypophosphite monohydrate sodium dihydrogens of 1.7g bis- dissolving, is settled to 1000ml, adds 5ml pH to be 8.0 in every 1L transport water The phosphate buffered solution of 0.1M, mixes;
2) fish is put:Cordierite Pa Select-Committee adult fishes are put in the air bag equipped with transport water, traffic density is less than 6/liter;
3) gradient cooling:Plastic bag inflation adds water:The volume of water exceedes the 2/3 of air bag capacity, after oxygenation, carries out gradient drop Temperature, to be lowered the temperature as a gradient per 4 DEG C, each gradient continues 28-32 minutes, and most at last water temperature drops to 2 DEG C;
4) case:The air bag of cooling is put in two-ply foam case of the interlayer built with ice cube, amount on the rocks is according to haulage time Adjust, 8-12 hours transport 4-6kg on the rocks;
5) entrucking transport;
6) water is changed:Transportation changes the water and oxygenation for adding buffer often more than 12 hours.
CN201610993247.7A 2016-11-08 2016-11-08 Long-distance transportation method for euchiloglanis kishinouyei adult fishes Pending CN106561506A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610993247.7A CN106561506A (en) 2016-11-08 2016-11-08 Long-distance transportation method for euchiloglanis kishinouyei adult fishes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610993247.7A CN106561506A (en) 2016-11-08 2016-11-08 Long-distance transportation method for euchiloglanis kishinouyei adult fishes

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CN106561506A true CN106561506A (en) 2017-04-19

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110622891A (en) * 2019-10-30 2019-12-31 成都鲵肽生物科技有限公司 Glyptophan glyptosum bionic artificial propagation method for precious fishes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101019520A (en) * 2006-02-15 2007-08-22 苏州市太湖观赏鱼培育场 Pet fish surviving transportation method
CN101703026A (en) * 2009-11-17 2010-05-12 浙江海洋学院 Squid living body transportation method and special ink control chute
CN102037950A (en) * 2010-12-15 2011-05-04 浙江海洋学院 pH stabilizer for octopus living body transportation
CN104026060A (en) * 2014-06-24 2014-09-10 张振寿 Packaging method for conveying Guandong fishes
CN104642229A (en) * 2015-03-12 2015-05-27 中国水产科学研究院长江水产研究所 Method for transporting and domesticating plateau fish seedlings

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101019520A (en) * 2006-02-15 2007-08-22 苏州市太湖观赏鱼培育场 Pet fish surviving transportation method
CN101703026A (en) * 2009-11-17 2010-05-12 浙江海洋学院 Squid living body transportation method and special ink control chute
CN102037950A (en) * 2010-12-15 2011-05-04 浙江海洋学院 pH stabilizer for octopus living body transportation
CN104026060A (en) * 2014-06-24 2014-09-10 张振寿 Packaging method for conveying Guandong fishes
CN104642229A (en) * 2015-03-12 2015-05-27 中国水产科学研究院长江水产研究所 Method for transporting and domesticating plateau fish seedlings

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张桂枝 等: "《规模禽场实验室工作手册》", 31 October 2013, 金盾出版社 *
申玉春: "《鱼类增养殖学》", 31 July 2008, 中国农业出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110622891A (en) * 2019-10-30 2019-12-31 成都鲵肽生物科技有限公司 Glyptophan glyptosum bionic artificial propagation method for precious fishes

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Effective date of registration: 20191223

Address after: East Lake New Technology Development Zone Wu Da Yuan Road 430223 in Hubei Province, Wuhan City, No. 8

Applicant after: YANGTZE RIVER FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES

Applicant after: YALONG RIVER HYDROPOWER DEVELOPMENT Co.,Ltd.

Address before: East Lake New Technology Development Zone Wu Da Yuan Road 430223 in Hubei Province, Wuhan City, No. 8

Applicant before: YANGTZE RIVER FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES

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Application publication date: 20170419

RJ01 Rejection of invention patent application after publication