CN106554903A - A kind of medicament evenly mixing device and its using method - Google Patents
A kind of medicament evenly mixing device and its using method Download PDFInfo
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- CN106554903A CN106554903A CN201611023997.8A CN201611023997A CN106554903A CN 106554903 A CN106554903 A CN 106554903A CN 201611023997 A CN201611023997 A CN 201611023997A CN 106554903 A CN106554903 A CN 106554903A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The present invention provides a kind of medicament evenly mixing device and its using method, the device will be shifted or the medicament that mixes is put in specimen port, under the action of the centrifugal force, by the steering of pedestal, rotating speed and rotational time make medicament enter quantitative portion from specimen port, required medicament is carried out quantitatively, the steering of pedestal is adjusted again, rotating speed and rotational time control quantitative pharmacy enter mixing unit from quantitative portion by siphon micro-valve, so as to fully mix required medicament, medicament after mixing is discharged from outlet conduit, and then realize including that medicament injects, mixing, the PCR reaction pretreatment process that nucleic acid extraction and PCR reaction systems build.The medicament evenly mixing device that the present invention is provided passes through the adjustment of the steering, rotating speed and rotational time of the setting and pedestal of passage and siphon micro-valve simplifies the control of medicament mixing, and reliability is improved；The setting of passage and siphon micro-valve causes the simple structure of medicament evenly mixing device, prepares and assembly technology requirement reduction, so as to reduce preparing and testing cost.
Description
Technical field
The present invention relates to reagent handling device technical field, more particularly to a kind of medicament evenly mixing device and its using method.
Background technology
PCR (Polymerase Chain Reaction, polymerase chain reaction) is that a kind of amplification in vitro expands
The technology of specific DNA (deoxyribonucleic acid, DNA (deoxyribonucleic acid)) fragment.PCR is generally required in DNA amplification
Through high temperature solution chain reaction, three steps of annealing reaction and extension, the initial DNA molecular quantity after above three step
Double, now circulate for one；DNA molecular after multiplication becomes the template of next circulation, so circulation multiplication then
Go down, after 30-40 circulation, DNA molecular number will be amplified to nearly 109 times of initial value.Thus, it is directed to analysis and examines
The DNA molecular as analyte can be carried out the extensive amplification of specificity for the purpose tested, PCR, therefore, round pcr can
For the early diagnosiss of infectious disease, hereditary and tumor etc., meanwhile, obtain more and more wider in prenatal diagnosiss and forensic identification
General application.
The molecular detecting method of PCR-based technology generally needs to experience sample process, nucleic acid extraction and PCR reaction systems
The processes such as structure, pcr amplification reaction and signal detection.Relative to pcr amplification reaction and detection process, sample process and nucleic acid
The pretreatment process of extraction etc. needs to carry out what each fluids (sample, reagent etc.) such as quantitative, transfer, mixing, separation were operated
, easily there is fluid mixing and the phenomenon of pollution occur in complex steps.To avoid fluid from being contaminated, automatization is occurred in that
PCR pretreating devices.The PCR pretreating devices of automatization mainly by based on the redevelopment on liquid working station, using work
The syringe pump stood, positioning guide rail and according to applying in the liquid storage groove of working area arrangement, reaction vessel and other functional modules,
Realize the function such as the quantitative of liquid, transfer, mixing and separation in working area.Although the PCR pretreating devices of automatization can
Complete PCR pretreatment process, but it is the usual complex structure in liquid working station, bulky, need by batch processing to improve which
Treatment effeciency, thus it is generally used for large-scale inspection laboratory.
In recent years, with the development of technology and theory, it is believed that collecting sample is sent to central laboratory being concentrated
The mode of medical science detection should be changed, and diagnosis more should be carried out centered on person under inspection, should be while detection again
Depending on the communication with person under inspection, the information the linked up reference significance important for clinical decision has, thus adjoint diagnosis and treatment are (live
Detection) increasingly it is taken seriously.Integrated, easy based on the demand with diagnosis and treatment, detection technique and equipment and small-sized
Change is increasingly becoming new trend.At present, a kind of more new PCR pretreating devices are the PCR detections based on rotary control valve
Pattern, i.e., used in cartridge, rotary control valve controls connection and the cut-off of each chamber and centrally located reaction chamber；Rotation control
Valve processed is also associated with a syringe pump, and the syringe pump can be from pumping liquid in the chamber of connection to reaction chamber or from reaction chamber
It is middle that liquid is depressed in the chamber of connection, and then just can be located before completing PCR in cartridge by rotary control valve and syringe pump
The basic process of reason.But due at least needing 2 motors and rotary control valve based on the PCR detection patterns of rotary control valve, because
And increase control complexity of the rotary control valve to each chamber folding.Simultaneously as the complex structure of rotary control valve, difficult processing
Degree is big, thus causes the preparation cost of rotary control valve higher, is unfavorable for large-scale promotion.
The content of the invention
The present invention provides a kind of medicament evenly mixing device and its using method, with solve controlling organization when medicament mixes it is complicated,
Unmanageable problem.
In a first aspect, the medicament evenly mixing device that the present invention is provided includes pedestal and the cover plate for covering the pedestal, institute
Stating pedestal includes specimen port, quantitative portion, mixing unit and outlet conduit, wherein,
The distance of the specimen port, the quantitative portion, the mixing unit and the outlet conduit apart from the base central
Increase successively；
Volume of the volume of the specimen port more than the quantitative portion；
The specimen port includes first sample groove and the second sample slot；
The quantitative portion includes the first quantitative slot and the second quantitative slot；
The first sample groove is connected by passage with first quantitative slot；
Second sample slot is connected by passage with second quantitative slot；
The mixing unit by siphon micro-valve respectively with first quantitative slot, second quantitative slot and the outlet
Road is connected；
The passage and the siphon micro-valve are respectively positioned in the specimen port, the quantitative portion and the mixing unit away from institute
State one end of base central.
With reference in a first aspect, in the first possible implementation of first aspect, the specimen port also includes the 3rd sample
This groove, the quantitative portion also include the 3rd quantitative slot and mixing quantitative slot, and the 3rd quantitative slot is included along the footpath of the pedestal
To the 3rd quantitative slot of the first order, the 3rd quantitative slot of the second level and the 3rd quantitative slot of the third level that direction sets gradually；
3rd sample slot is connected with the 3rd quantitative slot of the first order；
The mixing unit includes the first mixing channel and the second mixing channel；
First mixing channel is connected with first quantitative slot, second quantitative slot respectively；
The mixing quantitative slot is connected with first mixing channel；
Second mixing channel is connected with the 3rd quantitative slot of the third level, the mixing quantitative slot respectively.
With reference in a first aspect, second of first aspect may in implementation,
The first sample groove is used to place sample liquid, and first quantitative slot is used for the quantitative sample liquid；
Second sample slot is used to place lysate, and second quantitative slot is used for the quantitative lysate；
3rd sample slot is used to place MIX liquid, and the 3rd quantitative slot is used for the quantitative MIX liquid；
First mixing channel is for the sample liquid and the lysate after mixing quantitatively；
The mixed liquor that the mixing quantitative slot is used in quantitative first mixing channel；
Second mixing channel be used to mixing mixed liquor in first mixing channel and it is quantitative after the MIX liquid.
With reference in a first aspect, in the third possible implementation of first aspect, described device also includes waste liquid tank, institute
State waste liquid tank respectively with first quantitative slot, second quantitative slot, the 3rd quantitative slot of the first order and described mix fixed
Measuring tank is connected.
With reference in a first aspect, in the 4th kind of possible implementation of first aspect, described device also includes being respectively provided with
Limiting section and Handheld Division on the pedestal opposite sides side, the limiting section are connected with the outlet conduit；The limit
Position portion also includes the through hole adjacent with the pedestal and the limited mouth away from the pedestal.
Second aspect, the present invention provide a kind of using method of medicament evenly mixing device, and methods described includes：
Injected into the specimen port including first sample groove and the second sample slot on pedestal by the through hole on cover plate and treated
Mixing sample；
Starting motor rotates pedestal；
Control the pedestal steering, rotating speed and rotation time make the sample to be mixed from the specimen port enter include
The quantitative portion of the first quantitative slot and the second quantitative slot, it is quantitative after obtain quantitative sample；
Steering, rotating speed and the rotation time for changing the pedestal makes the quantitative sample copy enter mixing from the quantitative portion
Portion, obtains mixing sample after mixing；
Steering, rotating speed and the rotation time for controlling the pedestal makes the mixing sample discharge from outlet conduit.
With reference to second aspect, in the first possible implementation of second aspect, by the through hole on cover plate to pedestal
On specimen port in injection sample to be mixed include：
It is injected separately into into the first sample groove on pedestal, the second sample slot and the 3rd sample slot by the through hole on cover plate
Sample liquid, lysate and MIX liquid, wherein, the MIX liquid includes PCR buffer and/or enzyme.
With reference to second aspect, in second possible implementation of second aspect, steering, the rotating speed of the pedestal are controlled
Make the sample to be mixed enter quantitative portion from the specimen port with rotation time, it is quantitative after obtain quantitative sample and include：
Control the pedestal rotate counterclockwise；
The sample liquid enters the first quantitative slot from the first sample groove, obtains quantitative sample liquid；
The lysate enters the second quantitative slot from second sample slot, obtains quantitative cleavage liquid；
The MIX liquid enters the 3rd quantitative slot of the first order from the 3rd sample slot, obtains quantitative MIX liquid；
The unnecessary sample liquid, the lysate and the MIX liquid enter waste liquid tank.
With reference to second aspect, in the third possible implementation of second aspect, change steering, the rotating speed of the pedestal
The quantitative sample copy is made to enter mixing unit from the quantitative portion with rotation time, mixing sample is obtained after mixing to be included：
Control the pedestal to turn clockwise, so that the quantitative sample liquid and the quantitative cleavage liquid are into the first mixing
Groove, obtains mixed liquor；The quantitative MIX liquid enters the 3rd quantitative slot of the second level；
The rotating speed for controlling the pedestal is slowly adjusted to 3600-4200rpm from 800-1200rpm, then from 3600-
4200rpm is slowly dropped to 800-1200rpm, so that the mixed liquor mix homogeneously, wherein, adjustment number of times is 8-12 time；
The pedestal rotate counterclockwise is controlled, so that the mixed liquor enters mixing quantitative slot, the quantitative MIX liquid is entered
The 3rd quantitative slot of the third level；
Control the pedestal to turn clockwise, so that the mixed liquor and the quantitative MIX liquid enter the second mixing channel, shape
Into mixing sample；
The rotating speed for controlling the pedestal is adjusted to 3600-4200rpm from 800-1200rpm, then slow from 3600-4200rpm
Slowly 800-1200rpm is down to, so that the mixing sample mix homogeneously, wherein, adjustment number of times is 8-12 time.
With reference to second aspect, in the 4th kind of possible implementation of second aspect, control the steering of the pedestal, turn
Speed and rotation time make the mixing sample discharge from outlet conduit to be included：
Control the pedestal to rotate counterclockwise, the mixing sample is discharged from outlet conduit.
The technical scheme that embodiments of the invention are provided can include following beneficial effect：
The medicament evenly mixing device that the present invention is provided includes pedestal and the cover plate for covering the pedestal, and the pedestal includes
Specimen port, quantitative portion, mixing unit and outlet conduit, wherein, the specimen port, the quantitative portion, the mixing unit and it is described go out
Mouth pipeline increases successively apart from the distance of the base central；Volume of the volume of the specimen port more than the quantitative portion；Institute
Stating specimen port includes first sample groove and the second sample slot；The quantitative portion includes the first quantitative slot and the second quantitative slot；It is described
First sample groove is connected by passage with first quantitative slot；Second sample slot and second quantitative slot are by logical
Road is connected；The mixing unit by siphon micro-valve respectively with first quantitative slot, second quantitative slot and the outlet
Pipeline is connected；The passage and the siphon micro-valve are respectively positioned in the specimen port, the quantitative portion and the mixing unit far
From one end of the base central.In the medicament evenly mixing device that the present invention is provided, it would be desirable to which transfer or the medicament for mixing are put into
Include the specimen port of first sample groove and the second sample slot, under the centrifugal force that pedestal is rotated, adjust steering, the rotating speed of pedestal
And rotational time control medicament by passage between specimen port and quantitative portion from first sample groove enter the first quantitative slot, second
Sample slot enters the second quantitative slot, so as to carry out to required medicament quantitatively, adjusts steering, rotating speed and the rotational time of pedestal again
Medicament after control is quantitative enters mixing unit from quantitative portion by the siphon micro-valve between quantitative portion and mixing unit, so as to fully mix
Medicament needed for even, the medicament after mixing are discharged from outlet conduit, and then realize including medicament injection, mixing, nucleic acid extraction and PCR
The PCR reaction pretreatment process that reaction system builds.The medicament evenly mixing device that the present invention is provided passes through passage and siphon micro-valve
Setting and the adjustment of the steering of pedestal, rotating speed and rotational time cause the control of medicament mixing to simplify, reliability raising；Together
When, the setting of passage and siphon micro-valve causes the simple structure of medicament evenly mixing device, prepares and assembly technology requirement reduction, so as to
Reduce preparing and testing cost.
It should be appreciated that the general description of the above and detailed description hereinafter are only exemplary and explanatory, not
The present invention can be limited.
Description of the drawings
During accompanying drawing herein is merged in description and the part of this specification is constituted, show the enforcement for meeting the present invention
Example, and be used for explaining the principle of the present invention together with description.
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, for those of ordinary skill in the art
Speech, without having to pay creative labor, can be with according to these other accompanying drawings of accompanying drawings acquisition.
Fig. 1 is dimensional structure diagram a of medicament evenly mixing device provided in an embodiment of the present invention；
Fig. 2 is dimensional structure diagram b of medicament evenly mixing device provided in an embodiment of the present invention；
Fig. 3 is the plane graph of medicament evenly mixing device provided in an embodiment of the present invention；
Fig. 4 be I in Fig. 1 provided in an embodiment of the present invention at enlarged drawing；
Fig. 5 is medicament evenly mixing device using method flow chart provided in an embodiment of the present invention；
Symbol is represented：
1- pedestals, 2- cover plates, 3- outlet conduits, 4- first sample grooves, the second sample slots of 5-, the first quantitative slots of 6-, 7-
Two quantitative slots, the 3rd sample slots of 8-, the 3rd quantitative slots of 9-, 10- mixing quantitative slots, the first mixing channels of 11-, the second mixing channels of 12-,
13- waste liquid tanks, 14- limiting sections, 15- Handheld Divisions, 16- through holes, 17- limited mouths, the 3rd quantitative slot of the 91- first order, the 92- second level
3rd quantitative slot, the 3rd quantitative slot of the 93- third level.
Specific embodiment
Here in detail exemplary embodiment will be illustrated, its example is illustrated in the accompanying drawings.Explained below is related to
During accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawings represent same or analogous key element.Following exemplary embodiment
Described in embodiment do not represent and the consistent all embodiments of the present invention.Conversely, they be only with as appended by
The example of consistent apparatus and method in terms of some described in detail in claims, the present invention.
Accompanying drawing 1-3 is refer to, accompanying drawing 1-3 respectively illustrates the not homonymy of medicament evenly mixing device provided in an embodiment of the present invention
The dimensional structure diagram and plane graph in face.
Medicament evenly mixing device provided in an embodiment of the present invention includes pedestal 1 and the cover plate 2 for covering pedestal 1, and pedestal 1 is wrapped
Specimen port, quantitative portion, mixing unit and outlet conduit 3 are included, wherein, specimen port, quantitative portion, mixing unit and outlet conduit 3 are apart from base
The distance at 1 center of seat increases successively；Volume of the volume of specimen port more than quantitative portion；Specimen port includes first sample groove 4 and
Two sample slots 5；Quantitative portion includes the first quantitative slot 6 and the second quantitative slot 7；First sample groove 4 and the first quantitative slot 6 pass through passage
It is connected；Second sample slot 5 is connected by passage with the second quantitative slot 7；Mixing unit is fixed with first respectively by siphon micro-valve
Measuring tank 6, the second quantitative slot 7 and outlet conduit 3 are connected；Passage and siphon micro-valve are respectively positioned on specimen port, quantitative portion and mixing unit
On away from 1 center of pedestal one end.
In medicament evenly mixing device provided in an embodiment of the present invention, pedestal 1 and cover plate 2 are single injection-molded, pedestal 1
In passage and outlet conduit 3 be the less pipeline of diameter, the siphon micro-valve in pedestal 1 for capillary dimensionss U-shaped microflow channels
Small sized pipeline, the second sample slot 5 and the second quantitative slot in road, such as accompanying drawing 1-3, between first sample groove 4 and the first quantitative slot 6
Small sized pipeline between 7 etc. is passage；U-tube road, the second quantitative slot 7 between first quantitative slot 6 and the first mixing channel 11
And first the U-tube road between mixing channel 11 etc. be siphon micro-valve.During 1 injection mo(u)lding of pedestal formed specimen port, quantitative portion and
Mixing unit, the passage, siphon micro-valve and outlet conduit 3 in pedestal 1 are realized through the secondary operations such as photoetching or etching.On cover plate 2
Be provided with plate hole, the plate hole for adding various medicaments in the specimen port of pedestal 1, thus, pedestal 1 and cover plate 2 are by ultrasound
When wave soldering connects to form one, plate hole needs are corresponding with the position of specimen port, and then reach sample-adding and the purpose for sealing.
In medicament evenly mixing device provided in an embodiment of the present invention, specimen port, quantitative portion, mixing unit and outlet conduit 3 away from
Increase with a distance from 1 center of pedestal successively, so that medicament can enter quantitative portion from specimen port in the presence of pedestal centrifugal force,
Mixing unit is being entered by quantitative portion, is finally being discharged by the outlet conduit 3 being connected with mixing unit.Further, the volume of specimen port
The volume more than quantitative portion is needed, and the volume in quantitative portion is fixed volume, being capable of Jing hence into a large amount of medicaments of specimen port
The quantitative portion for crossing fixed volume carries out accurate quantitative analysis, and can avoid operating trace medicament to cause quantitative deviation larger, and then obtains
The intermediate product yield of get Geng Gao, while also not interfering with the final volume of PCR reaction systems.
Further, distance of the two ends of specimen port, quantitative portion and mixing unit apart from 1 center of pedestal is different, so sets
Put the medicament that can be easy in specimen port, quantitative portion and mixing unit and specimen port, quantitative is pooled in the presence of 1 centrifugal force of pedestal
Portion and one end of mixing unit.Due to more remote with a distance from 1 center of pedestal, centrifugal force is bigger, therefore, for enable medicament all by
Specimen port enters mixing unit into quantitative portion or by quantitative portion, and passage and siphon micro-valve are arranged at specimen port, determine when arranging
In amount portion and mixing unit away from 1 center of pedestal one end, and then be easy to the conveying of solvent and air in quantitative portion and mixing unit
Discharge.
In medicament evenly mixing device provided in an embodiment of the present invention, specimen port includes first sample groove 4 and the second sample slot
5, quantitative portion includes the first quantitative slot 6 and the second quantitative slot 7, wherein, first sample groove 4 and the second sample slot 5 are used to place each
Medicament is planted, the medicament that the first quantitative slot 6 is used in quantitative first sample groove 4, the second quantitative slot 7 are used for quantitative second sample slot 5
Interior medicament.Specimen port is connected by passage between the two with quantitative portion, i.e., first sample groove 4 and the first quantitative slot 6 lead to
Cross passage to be connected；Second sample slot 5 is connected by passage with the second quantitative slot 7, so as to realize that medicament is entered from specimen port
Quantitative portion.As the first quantitative slot 6 and the second quantitative slot 7 are connected with mixing unit respectively by siphon micro-valve, and stop in pedestal 1
After rotation stop is dynamic, siphon micro-valve can produce siphonage, thus, it is quantitative after medicament can be fixed from first respectively by siphon micro-valve
Measuring tank 6 and the second quantitative slot 7 enter mixing unit.As mixing unit is connected by siphon micro-valve with outlet conduit 3, thus, respectively
Discharged by outlet conduit 3 after the blended portion's mixing of medicament.
In embodiments of the present invention, medicament is that PCR buffer, enzyme, lysate, serum, blood plasma or whole blood etc. are raw
Thing medicament, certainly, medicament can also be various chemical agents and other biological medicament for needing quantitatively mixing etc., and the present invention is to needing
The medicament quantitatively to mix is not intended to limit.A kind of medicament can not only be injected in first sample groove 4 and the second sample slot 5, and
Various different medicaments can also be injected, to realize the injection of various medicaments and mix.
In medicament evenly mixing device provided in an embodiment of the present invention, it would be desirable to which transfer or the medicament for mixing are put into and include the
The specimen port of one sample slot 4 and the second sample slot 5, realizes the injection of sample；Under the centrifugal force that pedestal 1 is rotated, pedestal 1 is adjusted
Steering, rotating speed and rotational time control medicament the is entered from first sample groove 4 by passage between specimen port and quantitative portion
Certain measuring tank 6, the second sample slot 5 enter the second quantitative slot 7, so as to carry out to required medicament quantitatively, adjust turning for pedestal 1 again
Entered from quantitative portion to the medicament of, rotating speed and rotational time control after quantitative by the siphon micro-valve between quantitative portion and mixing unit
Mixing unit, so as to fully mix required medicament, the medicament after mixing is discharged from outlet conduit 3, and then realize include medicament inject,
The PCR reaction pretreatment process of mixing, nucleic acid extraction and PCR reaction systems structure etc..Medicament provided in an embodiment of the present invention is mixed
The adjustment of the steering, rotating speed and rotational time of setting and pedestal 1 of the even device by passage and siphon micro-valve causes medicament to mix
The control of conjunction is simplified, and reliability is improved；Meanwhile, the setting of passage and siphon micro-valve causes the structure letter of medicament evenly mixing device
Single, preparation and assembly technology require to reduce, so as to reduce preparing and testing cost.
In medicament evenly mixing device provided in an embodiment of the present invention, specimen port also includes the 3rd sample slot 8, and quantitative portion also includes
3rd quantitative slot 9 and mixing quantitative slot 10, mixing unit include the first mixing channel 11 and the second mixing 12, wherein, the 3rd sample slot 8
For placing medicament, the medicament that the 3rd quantitative slot 9 is used in quantitative 3rd sample slot 8, the first mixing channel 11 are used for mixing first
Medicament in quantitative slot 6 and the second quantitative slot 7, mixes the confection that quantitative slot 10 is used in quantitative first mixing channel 11, the
Two mixing 12 are used for the 3rd quantitative slot 9 of mixing and mix the medicament in quantitative slot 10.As the medicament in specimen port is quantitative, first
Quantitative slot 6 and the second quantitative slot 7 mix, it is quantitative after, be the further mixing for controlling medicament, the steering of pedestal 1 is required to change,
Dimension needs control pedestal 1 to stop operating, and now siphon micro-valve can produce siphonage, to prevent the medicine in the 3rd quantitative slot 9
Agent affects the mixing of the first quantitative slot 6 and the second quantitative slot 7, quantitative, so that arranging multiple 3rd quantitative slots 9.At this
In bright embodiment, the 3rd quantitative slot 92 of the 3rd quantitative slot 91, second level of the first order and are set gradually along the radial direction of pedestal 1
Three-level 93 3 the 3rd quantitative slots 9 of the 3rd quantitative slot.The annexation of above-mentioned each specimen port, quantitative portion and mixing unit is：3rd
Sample slot 8 is connected with the 3rd quantitative slot 91 of the first order, and the first quantitative 6 and second quantitative slot 7 is connected with the first mixing channel 11
Logical, mixing quantitative 10 is connected with the first mixing 11, the second mixing channel 12 respectively with the 3rd quantitative slot 93 of the third level, mix quantitatively
Groove 10 is connected, and the 3rd quantitative slot of the second level 92 connects the 3rd quantitative slot 91 of the first order and the 3rd quantitative slot 93 of the third level, above-mentioned
Connection between each part is realized by siphon micro-valve.
Further, in embodiments of the present invention, the sample liquid of serum, blood plasma or whole blood etc. is placed in first sample groove 4,
Lysate is placed in second sample slot 5, PCR buffer and/or the enzyme MIX liquid that waits in the 3rd sample slot 8, is placed, then first
6 quantitative sample liquid of quantitative slot, 7 quantitative cleavage liquid of the second quantitative slot, 9 quantitative MIX liquid of the 3rd quantitative slot；First mixing channel 11 is used for
Sample liquid and lysate after mixing is quantitative, forms sample cracking mixed liquor；It is mixed that mixing quantitative slot 10 is used for the cracking of quantitative sample
Close liquid, the second mixing channel be used to mixing it is quantitative after MIX liquid and it is quantitative after sample cracking mixed liquor, so as to form sample cracking
MIX mixed liquors, i.e. PCR reaction systems sample.
Further, medicament evenly mixing device provided in an embodiment of the present invention also include waste liquid tank 13, waste liquid tank 13 respectively with
First quantitative slot 6, the second quantitative slot 7, the 3rd quantitative slot 91 of the first order are connected with mixing quantitative slot 10, quantitative in order to first
Unnecessary medicament is entered by groove 6, the second quantitative slot 7, the 3rd quantitative slot 91 of the first order and mixing quantitative slot 10 when carrying out quantitative
In waste liquid tank 13.
As the steps such as the medicament injection in PCR reaction system forming processes, mixing, nucleic acid extraction are at sample analyses
Reason the instrument last time complete, consequently, to facilitate the PCR reaction system samples after blended process need by siphon micro-valve via
Outlet conduit 3 is expelled in PCR reaction tubes, and PCR reaction tubes need to rotate together with medicament evenly mixing device.To prevent PCR from reacting
There is the medicament inlet port of PCR reaction tubes in pipe during rotation and outlet conduit 3 misplaces, depart from or PCR reaction tubes are from medicine
Situation about coming off on agent evenly mixing device occurs, it is therefore desirable to by the restriction PCR reaction tubes of limiting section 14 on medicament evenly mixing device
Position, wherein, limiting section 14 is arranged on the side of medicament evenly mixing device, and limiting section 14 is connected with outlet conduit 3.Enter
One step, can also be fixed on PCR reaction tubes on medicament evenly mixing device by way of ultrasonic bonding.For ease of medicament is mixed
Even device is positioned on sample analyses process instrumentation, and Handheld Division 15 is additionally provided with the side of medicament evenly mixing device.Work as limiting section
14 and Handheld Division 15 when being not at symmetric position on medicament evenly mixing device, pedestal 1 easily occurs centrifugation during rotation
The uneven phenomenon of power, so that medicament evenly mixing device occurs vibrations on sample analyses process instrumentation, affects the mixer of medicament
And it is quantitative, therefore, in embodiments of the present invention, limiting section 14 and Handheld Division 15 are separately positioned on two relative sides of pedestal 1.
Accompanying drawing 4 is refer to, accompanying drawing 4 shows the enlarged drawing of limiting section provided in an embodiment of the present invention 14.Limiting section 14 pairs
When the position of PCR reaction tubes is defined, the body of PCR reaction tubes is limited away from the limited mouth 17 of pedestal 1, it is adjacent with pedestal 1
Through hole 16 limit PCR reaction tubes lid.The size of limited mouth 17 and through hole 16, shape are determined according to PCR reaction tubes.
The shape of pedestal 1, size can be determined according to concrete detection demand, in medicament provided in an embodiment of the present invention
In evenly mixing device, pedestal 1 is set to the circle that a diameter of 5.1cm, thickness are 5.2mm.The shape of specimen port, quantitative portion and mixing unit
Shape, volume equally can be determined according to concrete detection demand, but the volume of specimen port have to be larger than the volume in quantitative portion, at this
In the medicament evenly mixing device that inventive embodiments are provided, according to the setting of pedestal 1, the volume of first sample groove 4 is 60-90 μ l, the
The volume of two sample slots 5 is 30-60 μ l, and the volume of the 3rd sample slot 8 is 60-140 μ l.
In medicament evenly mixing device provided in an embodiment of the present invention, listing specimen port respectively includes two sample slots (
One sample slot 4 and the second sample slot 5) and three sample slots (first sample groove 4, the second sample slot 5 and the 3rd sample slot 8) feelings
Shape, but medicament evenly mixing device provided in an embodiment of the present invention is not limited to the specimen port of above two form, can arrange four
Individual or multiple specimen ports, while arrange corresponding with specimen port quantitative portion and mixing unit, and then realize the mixing of various medicaments with
Quantitatively.When arranging, the distance of specimen port, quantitative portion and mixing unit apart from 1 center of pedestal must increase multiple specimen ports etc. successively
Greatly, so that medicament can smoothly successively via be expelled to after specimen port, quantitative portion and mixing unit PCR reaction tubes or other dress
In putting.
Accompanying drawing 5 is refer to, accompanying drawing 5 shows the using method flow process of medicament evenly mixing device provided in an embodiment of the present invention
Figure, following specifically used methods are based on accompanying drawing 5.
The using method of medicament evenly mixing device provided in an embodiment of the present invention includes：
S01：Noted into the specimen port including first sample groove and the second sample slot on pedestal by the through hole on cover plate
Enter sample to be mixed；
S02：Starting motor rotates pedestal；
S03：Steering, rotating speed and the rotation time for controlling the pedestal makes the sample to be mixed enter from the specimen port
Including the first quantitative slot and the quantitative portion of the second quantitative slot, it is quantitative after obtain quantitative sample；
S04：Steering, rotating speed and the rotation time for changing the pedestal makes the quantitative sample copy enter from the quantitative portion
Mixing unit, obtains mixing sample after mixing；
S05：Steering, rotating speed and the rotation time for controlling the pedestal makes the mixing sample discharge from outlet conduit.
Above-mentioned using method is specifically described as：
S01：Noted into the specimen port including first sample groove and the second sample slot on pedestal by the through hole on cover plate
Enter sample to be mixed；
It is fixed to medicament evenly mixing device parallel on sample analyses process instrumentation, by the through hole on cover plate 2 on pedestal 1
First sample groove 4, be injected separately into sample liquid, lysate and MIX liquid in the second sample slot 5 and the 3rd sample slot 8, wherein, sample
This liquid is serum, blood plasma or whole blood etc., and MIX liquid includes PCR buffer and/or enzyme etc..
S02：Start the motor on sample analyses process instrumentation, make pedestal 1 rotate；
S03：Steering, rotating speed and the rotation time for controlling the pedestal makes the sample to be mixed enter from the specimen port
Including the first quantitative slot and the quantitative portion of the second quantitative slot, it is quantitative after obtain quantitative sample；
Rotate counterclockwise 60-180s under conditions of rotating speed is 4500-6500rpm of pedestal 1 is controlled, so as to sample liquid, split
Solution liquid and MIX liquid enter the first quantitative slot 6, the second sample slot 5 from first sample groove 4 under the influence of centrifugal force respectively and enter the
Two quantitative slots 7 and the 3rd sample slot 8 enter the 3rd quantitative slot 91 of the first order, and then respectively obtain quantitative sample liquid, quantitative cleavage
Liquid and quantitative MIX liquid.Preferably, the rotating speed of pedestal 1 be 5000rpm, rotational time is 120s, now, sample liquid, lysate and
MIX liquid energies are enough completely filled with the first quantitative slot 6, the 3rd quantitative slot 91 of the second quantitative slot 7 and the first order.Unnecessary sample liquid, split
Solution liquid and MIX liquid enter waste liquid tank 13 by the first quantitative slot 6, the second quantitative slot 7 and the 3rd quantitative slot 91 of the first order respectively.The
Air in certain measuring tank 6, the 3rd quantitative slot 91 of the second quantitative slot 7 and the first order is then via the rainbow of each quantitative slot outermost radial outside
Inhale micro-valve to discharge.
S04：Steering, rotating speed and the rotation time for changing the pedestal makes the quantitative sample copy enter from the quantitative portion
Mixing unit, obtains mixing sample after mixing；
After the completion of sample liquid, lysate and MIX liquid are quantitative, stop pedestal 1 and rotate.It is 4500- that pedestal 1 is controlled in rotating speed
Turn clockwise under conditions of 6500rpm 10-30s, the siphonage power of quantitative sample liquid and quantitative cleavage liquid in siphon micro-valve
Under drain emptying in the first mixing channel 11, obtain mixed liquor；Likewise, siphonage power of the quantitative MIX liquid in siphon micro-valve
Under drain emptying in the 3rd quantitative slot 92 of the second level.Preferably, the rotating speed of pedestal 1 is 5000rpm, and rotational time is 10s, this
When, quantitative sample liquid and quantitative cleavage liquid energy are enough emptied completely in the first mixing channel 11, and quantitative MIX liquid energies are enough emptied completely
Into in the 3rd quantitative slot 92 of the second level.
The rotating speed rapidly near 800-1200rpm of control pedestal 1, the rotating speed for then controlling pedestal 1 are slow from 800-1200rpm
Slowly improve to 3600-4200rpm, above-mentioned speed adjustment modes repeat 8-12 time, so that mixed liquor is mixed in the first mixing channel 11
Close uniform.Preferably, the adjustment of rotational speed of pedestal 1 changes between 1000rpm and 4000rpm, and speed adjustment modes are repeated 12 times.
Mixed liquor controls pedestal 1 after being thoroughly mixed and stops operating.
Rotate counterclockwise 10-30s under conditions of rotating speed is 2500-3500rpm of pedestal 1 is controlled, so that mixed liquor is in rainbow
Mixing quantitative slot 10 is drained emptying under the siphonage power for inhaling micro-valve, quantitative MIX liquid is arranged under the siphonage power of siphon micro-valve
It is empty to enter the 3rd quantitative slot 93 of the third level.Preferably, the rotating speed of pedestal 1 is 3000rpm, and rotational time is 10s.
Control pedestal 1 to turn clockwise 10-30s, mixed liquor and quantitative MIX under conditions of rotating speed is 2500-3500rpm
Liquid enters the second mixing channel 12, forms mixing sample.Preferably, the rotating speed of pedestal 1 is 3000rpm, and rotational time is 10s.
The rotating speed rapidly near 800-1200rpm of control pedestal 1, the rotating speed for then controlling pedestal 1 are carried from 800-1200rpm
Up to 3600-4200rpm, above-mentioned speed adjustment modes repeat 8-12 time, so that mixing sample mixes in the second mixing channel 12
Uniformly.Preferably, the adjustment of rotational speed of pedestal 1 changes between 1000rpm and 4000rpm, and speed adjustment modes are repeated 12 times.
S05：Steering, rotating speed and the rotation time for controlling pedestal makes the mixing sample discharge from outlet conduit.
Control pedestal 1 and 30-60s is rotated counterclockwise under conditions of rotating speed is 2500-3500rpm, make mixing sample from going out
Mouth pipeline 3 is expelled in PCR reaction tubes, so as to complete the structure of PCR reaction systems.Preferably, the rotating speed of pedestal 1 is
3000rpm, rotational time are 40s.
Medicament evenly mixing device provided in an embodiment of the present invention based in the presence of centrifugal force, by arranging first sample
The mode of the lead-over grooves such as groove, the second sample slot, completes the mixing and reaction of medicament step by step.For participation end reaction small volume
Medicament, the embodiment of the present invention are provided with two grades or multistage quantitative slot, with the accurate quantitative analysis medicament.The medicament provided in the present invention is mixed
In even device, the rotation of pedestal 1 needs only to a driving machine and drives, and then can reduce energy consumption and reduce answering for control technique
Miscellaneous degree.In addition, the adjustment of the steering, rotating speed and rotational time of setting and pedestal by passage and siphon micro-valve causes medicine
The control of agent mixing is simplified, and reliability is improved；Meanwhile, the setting of passage and siphon micro-valve causes the structure of medicament evenly mixing device
Simply, prepare and assembly technology requires to reduce, so as to reduce preparing and testing cost.
It should be noted that herein, the relational terms of such as " first " and " second " or the like are used merely to one
Individual entity or operation are made a distinction with another entity or operation, and are not necessarily required or implied these entities or operate it
Between there is any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Cover including for nonexcludability, so that a series of process, method, article or equipment including key elements not only includes those
Key element, but also including other key elements being not expressly set out, or also include for this process, method, article or set
Standby intrinsic key element.In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that
Also there is other identical element in the process including the key element, method, article or equipment.
Those skilled in the art will readily occur to its of the present invention after considering description and putting into practice disclosure of the invention here
Its embodiment.The application is intended to any modification of the present invention, purposes or adaptations, these modifications, purposes or
Person's adaptations follow the general principle of the present invention and including the undocumented common knowledge in the art of the present invention
Or conventional techniques.Description and embodiments are considered only as exemplary, and true scope and spirit of the invention are by following
Claim is pointed out.
It should be appreciated that the precision architecture for being described above and being shown in the drawings is the invention is not limited in, and
And various modifications and changes can be being carried out without departing from the scope.The scope of the present invention is limited only by appended claim.
Claims (10)
1. a kind of medicament evenly mixing device, it is characterised in that described device includes pedestal (1) and for covering the pedestal (1)
Cover plate (2), the pedestal (1) include specimen port, quantitative portion, mixing unit and outlet conduit (3), wherein,
The specimen port, the quantitative portion, the mixing unit and the outlet conduit (3) apart from the pedestal (1) center away from
From increasing successively；
Volume of the volume of the specimen port more than the quantitative portion；
The specimen port includes first sample groove (4) and the second sample slot (5)；
The quantitative portion includes the first quantitative slot (6) and the second quantitative slot (7)；
The first sample groove (4) is connected by passage with first quantitative slot (6)；
Second sample slot (5) is connected by passage with second quantitative slot (7)；
The mixing unit by siphon micro-valve respectively with first quantitative slot (6), second quantitative slot (7) and the outlet
Pipeline (3) is connected；
The passage and the siphon micro-valve are respectively positioned in the specimen port, the quantitative portion and the mixing unit away from the base
The one end at seat (1) center.
2. medicament evenly mixing device according to claim 1, it is characterised in that the specimen port also includes the 3rd sample slot
(8), the quantitative portion also includes the 3rd quantitative slot (9) and mixing quantitative slot (10), and the 3rd quantitative slot (9) is included along described
The 3rd quantitative slot (91) of the first order, the 3rd quantitative slot (92) of the second level and the third level that the radial direction of pedestal (1) sets gradually
3rd quantitative slot (93)；
3rd sample slot (8) is connected with the 3rd quantitative slot (91) of the first order；
The mixing unit includes the first mixing channel (11) and the second mixing channel (12)；
First mixing channel (11) is connected with first quantitative slot (6), second quantitative slot (7) respectively；
Mixing quantitative slot (10) is connected with first mixing channel (11)；
Second mixing channel (12) is connected with the 3rd quantitative slot (93) of the third level, mixing quantitative slot (10) respectively
It is logical.
3. medicament evenly mixing device according to claim 2, it is characterised in that
, for placing sample liquid, first quantitative slot (6) is for the quantitative sample liquid for the first sample groove (4)；
, for placing lysate, second quantitative slot (7) is for the quantitative lysate for second sample slot (5)；
, for placing MIX liquid, the 3rd quantitative slot (9) is for the quantitative MIX liquid for 3rd sample slot (8)；
First mixing channel (11) is for the sample liquid and the lysate after mixing quantitatively；
Mixing quantitative slot (10) is for the mixed liquor in quantitative first mixing channel (11)；
Second mixing channel (12) for mix the mixed liquor in first mixing channel (11) and it is quantitative after the MIX
Liquid.
4. medicament evenly mixing device according to claim 3, it is characterised in that described device also includes waste liquid tank (13), institute
State waste liquid tank (13) respectively with first quantitative slot (6), second quantitative slot (7), the 3rd quantitative slot of the first order
(91) it is connected with mixing quantitative slot (10).
5. the medicament evenly mixing device according to any one in claim 1-4, it is characterised in that described device also includes point
The limiting section (14) not being arranged on the pedestal (1) opposite sides side and Handheld Division (15), the limiting section (14) with it is described
Outlet conduit (3) is connected；The limiting section (14) also includes the through hole (16) adjacent with the pedestal (1) and away from described
The limited mouth (17) of pedestal (1).
6. a kind of using method of medicament evenly mixing device, it is characterised in that methods described includes：
Inject to be mixed by the through hole on cover plate into the specimen port including first sample groove and the second sample slot on pedestal
Sample；
Starting motor rotates pedestal；
Control the pedestal steering, rotating speed and rotation time make the sample to be mixed from the specimen port enter include first
The quantitative portion of quantitative slot and the second quantitative slot, it is quantitative after obtain quantitative sample；
Steering, rotating speed and the rotation time for changing the pedestal makes the quantitative sample copy enter mixing unit from the quantitative portion,
Mixing sample is obtained after mixing；
Steering, rotating speed and the rotation time for controlling the pedestal makes the mixing sample discharge from outlet conduit.
7. the using method of medicament evenly mixing device according to claim 6, it is characterised in that by the through hole on cover plate to
In specimen port on pedestal, injection sample to be mixed includes：
Sample is injected separately into by the through hole on cover plate into the first sample groove on pedestal, the second sample slot and the 3rd sample slot
Liquid, lysate and MIX liquid, wherein, the MIX liquid includes PCR buffer and/or enzyme.
8. the using method of medicament evenly mixing device according to claim 7, it is characterised in that the control pedestal turns
Make the sample to be mixed enter quantitative portion from the specimen port to, rotating speed and rotation time, it is quantitative after obtain quantitative sample bag
Include：
Control the pedestal rotate counterclockwise；
The sample liquid enters the first quantitative slot from the first sample groove, obtains quantitative sample liquid；
The lysate enters the second quantitative slot from second sample slot, obtains quantitative cleavage liquid；
The MIX liquid enters the 3rd quantitative slot of the first order from the 3rd sample slot, obtains quantitative MIX liquid；
The unnecessary sample liquid, the lysate and the MIX liquid enter waste liquid tank.
9. the using method of medicament evenly mixing device according to claim 8, it is characterised in that change turning for the pedestal
Make the quantitative sample copy mixing unit be entered from the quantitative portion to, rotating speed and rotation time, mixing sample bag is obtained after mixing
Include：
Control the pedestal to turn clockwise, so that the quantitative sample liquid and the quantitative cleavage liquid enter the first mixing channel,
Obtain mixed liquor；The quantitative MIX liquid enters the 3rd quantitative slot of the second level；
The rotating speed for controlling the pedestal is slowly adjusted to 3600-4200rpm from 800-1200rpm, then slow from 3600-4200rpm
Slowly 800-1200rpm is down to, so that the mixed liquor mix homogeneously, wherein, adjustment number of times is 8-12 time；
The pedestal rotate counterclockwise is controlled, so that the mixed liquor enters mixing quantitative slot, the quantitative MIX liquid enters the 3rd
The 3rd quantitative slot of level；
Control the pedestal to turn clockwise, so that the mixed liquor and the quantitative MIX liquid enter the second mixing channel, form mixed
Close sample；
The rotating speed for controlling the pedestal is adjusted to 3600-4200rpm from 800-1200rpm, then is slowly dropped from 3600-4200rpm
To 800-1200rpm, so that the mixing sample mix homogeneously, wherein, adjustment number of times is 8-12 time.
10. the using method of medicament evenly mixing device according to claim 9, it is characterised in that the control pedestal turns
The mixing sample is discharged from outlet conduit to, rotating speed and rotation time includes：
Control the pedestal to rotate counterclockwise at a high speed, the mixing sample is discharged from outlet conduit.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611023997.8A CN106554903B (en) | 2016-11-14 | 2016-11-14 | A kind of medicament evenly mixing device and its application method |
| PCT/CN2016/112050 WO2018086207A1 (en) | 2016-11-14 | 2016-12-26 | Agent-mixing device and method of using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611023997.8A CN106554903B (en) | 2016-11-14 | 2016-11-14 | A kind of medicament evenly mixing device and its application method |
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| Publication Number | Publication Date |
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| CN106554903A true CN106554903A (en) | 2017-04-05 |
| CN106554903B CN106554903B (en) | 2019-10-08 |
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| CN201611023997.8A Active CN106554903B (en) | 2016-11-14 | 2016-11-14 | A kind of medicament evenly mixing device and its application method |
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| CN (1) | CN106554903B (en) |
| WO (1) | WO2018086207A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018086207A1 (en) * | 2016-11-14 | 2018-05-17 | 湖南圣湘生物科技有限公司 | Agent-mixing device and method of using same |
| CN109266518A (en) * | 2018-11-29 | 2019-01-25 | 奥然生物科技（上海）有限公司 | It is a kind of to be provided with micro-fluidic or nanometer flow control structure biological reaction apparatus |
| CN115074230A (en) * | 2022-07-21 | 2022-09-20 | 北京泰豪生物科技有限公司 | Reagent controlled release module, bioreactor and biological detection device |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110261595B (en) * | 2019-07-17 | 2022-07-15 | 安图实验仪器(郑州)有限公司 | Sample liquid diluting and uniformly mixing method suitable for chemiluminescence immunoassay analyzer |
| CN112875779A (en) * | 2021-02-11 | 2021-06-01 | 林臣鑫 | Municipal administration sewage treatment plant |
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| CN106554903B (en) | 2019-10-08 |
| WO2018086207A1 (en) | 2018-05-17 |
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Address after: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan Patentee after: Shengxiang Biotechnology Co., Ltd Address before: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan Patentee before: Sansure Biotech Inc. |