CN208270589U - Multiple determination chip - Google Patents
Multiple determination chip Download PDFInfo
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- CN208270589U CN208270589U CN201820733141.8U CN201820733141U CN208270589U CN 208270589 U CN208270589 U CN 208270589U CN 201820733141 U CN201820733141 U CN 201820733141U CN 208270589 U CN208270589 U CN 208270589U
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Abstract
The utility model discloses a kind of multiple determination chip, which includes: sample treatment unit；Liquid storage unit, the liquid storage unit includes the dilution storage area and dilution quantification area being connected, wherein, the dilution quantification area includes interconnected buffering sub-district, quantitative sub-district and waste liquid sub-district, buffer enters the quantitative sub-district through the buffering sub-district by the dilution storage area and is quantified, and the extra buffer enters the waste liquid sub-district；And reaction member, the reaction member are connected with the sample treatment unit and the quantitative sub-district, wherein the sample treatment unit, the liquid storage unit and the reaction member are communicated with the atmosphere.By the way that the reaction of the sample after the quantitative dilution of sample treatment, buffer and dilution is integrated, simplify operating process, and dilution is quantified and divides into interconnected buffering sub-district, quantitative sub-district and waste liquid sub-district, realize the accurate quantitative of dilution, detection efficiency and precision are high.
Description
Technical field
The utility model relates to analysis detection fields, and in particular, to multiple determination chip.
Background technique
Biological detection is the basic means of the various related fieldss such as premise and bio-safety of medical diagnosis.Automation
Quick detection technique is wherein important developing direction, has become disease quick diagnosis, health supervision, environment and food peace
Hot spot in full inspection survey.Automated analysis detection device used in Hospitals at Present and inspection body, as full-automatic biochemical is analyzed
Instrument and immunity analysis instrument complete various pipettings using manipulator, have the independent multiplicity of consumptive material that operating procedure is more, uses
The deficiencies of, and systematic comparison is huge, expensive, more demanding to user of service, is suitble to center type batch detection, is unsatisfactory for existing
Fast and accurately detection needs for field.And with the innovation progress of social development and Medical treatment system, family doctor and community clinic
The effect played in medical diagnosis on disease and health care will be increasing.These application scenarios want used detection technique
Asking has very big difference with large hospital and inspection body, and the batch detection demand of sample declines to a great extent, and to timeliness, standard
The requirement of true property and portability is strong, therefore the macro-organism detection system based on manipulator is unsuitable, and to a small amount of sample
The automatic detection of the high accuracy of " sample into-result go out " of product has strong demand.Therefore being badly in need of exploitation is suitble to scene fast
Portable, the automation, integrated detection equipment of speed detection.
The multiple determination chip of the automation of high accuracy requires study as a result,.
Utility model content
The utility model aims to solve at least one of the technical problems existing in the prior art.For this purpose, the utility model
One purpose is to propose a kind of multiple determination chip, by dilution is quantified divide into interconnected buffering sub-district,
Quantitative sub-district and waste liquid sub-district these three sub-districts, realize that the accurate of dilution quantifies, make the detection efficiency of multiple determination chip
It is significantly improved with detection accuracy.
Thus, one aspect according to the present utility model, the utility model provides a kind of multiple determination chip, special
Sign is, comprising: sample treatment unit；Liquid storage unit, the liquid storage unit include the dilution storage area being connected and dilution
Liquid quantification area, wherein the dilution quantification area includes interconnected buffering sub-district, quantitative sub-district and waste liquid sub-district, buffering
Liquid enters the quantitative sub-district through the buffering sub-district by the dilution storage area and is quantified, the extra buffer into
Enter the waste liquid sub-district；And reaction member, the reaction member are connected with the sample treatment unit and the quantitative sub-district,
Wherein, the sample treatment unit, the liquid storage unit and the reaction member are communicated with the atmosphere.
According to the multiple determination chip of the utility model embodiment, by sample treatment, the quantitative dilution of buffer and dilute
The reaction of sample after releasing integrates, and simplifies operating process, operates without professional, saves manpower.Also, it is logical
It crosses to quantify dilution and divides into interconnected buffering sub-district, quantitative sub-district and waste liquid sub-district these three sub-districts, realize dilution
The accurate of liquid quantifies, and significantly improves the detection efficiency of multiple determination chip and detection accuracy.
Optionally, the buffering sub-district is connected with the quantitative sub-district by the first contraction channel；The quantitative sub-district with
The waste liquid sub-district passes through the second contraction channel and is connected, and described first shrinks channel and the second contraction channel described in
Between quantitative sub-district and the centrifugation center of the multiple determination chip.
Optionally, the volume of the quantitative sub-district is 20 μ L-5ml, and the volume of the buffering sub-district is not less than described fixed
The half of the volume in quantum area.
Optionally, the longitudinal section of the buffering sub-district and the quantitative sub-district is trapezoidal.
Optionally, the area of section and the buffering sub-district of the first contraction channel and the buffering sub-district junction are flat
The ratio of equal radial cross-sectional area is 0.001-0.5, and described first shrinks the section face in channel and the quantitative sub-district junction
The ratio of the long-pending and described quantitative sub-district average radial area of section is 0.001-0.5.
Optionally, the area of section and the quantitative sub-district of the second contraction channel and the quantitative sub-district junction are flat
The ratio of equal radial cross-sectional area is 0.001-0.5, and described second shrinks the section face in channel and waste liquid sub-district junction
Long-pending and the waste liquid sub-district average radial area of section ratio is 0.001-0.5.
Optionally, the sample treatment unit includes: sample feeding pipe；Sample introduction prevents hole, the sample introduction prevent hole with it is described into
Sample pipe is connected by the first capillary pipeline, and the sample introduction prevents the diameter in hole greater than the diameter of first capillary pipeline；Sample
Product Quantitative Separation pond, the sample amounts separate tank prevent hole from being connected with the sample introduction.
Optionally, the reaction member includes: mixing pit；Distribution pipe, the distribution pipe includes: that siphon prevents hole, described
Siphon prevents hole from being connected with the mixing pit by the second capillary pipeline, and the siphon prevents the diameter in hole from being greater than described second
The diameter of capillary pipeline；Liquid dispensing tube, the liquid dispensing tube prevent hole from being connected with the siphon；Multiple reaction tanks, it is described
Multiple reaction tanks are connected with the liquid dispensing tube.
Optionally, the mixing pit includes: buffer area, and the buffer area and the sample treatment unit pass through third capillary
Pipeline is connected；And mixed zone, the mixed zone are connected with the free end of the buffer area.
Optionally, which further comprises: multiple grooves, and the multiple groove and the multiple reaction tank are in thickness side
It corresponds upwards, and the back side of the reaction tank is set.
Optionally, the edge circumferentially of the chip is serrated, and the recessed portion of the reaction tank and the sawtooth
Radially it is arranged in a one-to-one correspondence.
Optionally, the reaction member further comprises: reaction solution waste liquid pool, the reaction solution waste liquid pool and the distribution
Pipe is connected, and is located remotely from the end that the siphon prevents the distribution pipe in hole.
The additional aspect and advantage of the utility model will be set forth in part in the description, partially will be from following description
In become obvious, or recognized by the practice of the utility model.
Detailed description of the invention
The above-mentioned and/or additional aspect and advantage of the utility model from the description of the embodiment in conjunction with the following figures will
Become obvious and be readily appreciated that, in which:
Fig. 1 shows the multiple determination chip structure schematic diagram according to the utility model one embodiment；
Fig. 2 shows the multiple determination chip structure schematic diagram according to the utility model one embodiment；
Fig. 3 shows the partial structural diagram of the multiple determination chip according to the utility model one embodiment；
Fig. 4 shows the multiple determination chip structure schematic diagram according to the utility model one embodiment；
Fig. 5 shows the multiple determination chip structure schematic diagram according to the utility model one embodiment；
Fig. 6 A- Fig. 6 D shows the multiple determination chip structure schematic diagram according to the utility model one embodiment.
Specific embodiment
The embodiments of the present invention are described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning
Same or similar element or element with the same or similar functions are indicated to same or similar label eventually.Below by ginseng
The embodiment for examining attached drawing description is exemplary, and is only used for explaining the utility model, and should not be understood as to the utility model
Limitation.
In the description of the present invention, term " longitudinal direction ", " transverse direction ", "upper", "lower", "front", "rear", "left", "right",
The orientation or positional relationship of the instructions such as "vertical", "horizontal", "top", "bottom" is to be based on the orientation or positional relationship shown in the drawings,
It is merely for convenience of description the utility model rather than requires the utility model that must be constructed and operated in a specific orientation, therefore
It should not be understood as limiting the present invention.
It should be noted that term " first ", " second " are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be with
Explicitly or implicitly include one or more of the features.Further, in the description of the present invention, unless otherwise
Illustrate, the meaning of " plurality " is two or more.
Referring to Fig. 1-5 description according to the multiple determination chip 100 of the utility model embodiment.
As shown in Figure 1, the multiple determination chip 100 of the utility model embodiment includes sample treatment unit 110, liquid storage
Unit 120 and reaction member 130, thus, the chip by sample treatment, buffer quantitative dilution and dilution after sample it is anti-
It should integrate, simplify operating process, be operated without professional, save manpower.As shown in Fig. 2, practical according to this
Novel embodiment, liquid storage unit 120 include the dilution storage area 121 and dilution quantification area 122 being connected, wherein dilute
Releasing liquid quantification area 122 includes interconnected buffering sub-district 123, quantitative sub-district 124 and waste liquid sub-district 125, and buffer is by diluting
Liquid storage area 121 enters quantitative sub-district 124 through buffering sub-district 123 and is quantified, the quantitative accurate buffer of 124 quantification of sub-district
Into reaction member 130, sample is mixed with treated, and extra buffer enters waste liquid sub-district 125, in waste liquid sub-district 125
Storage.
It should be noted that sample treatment unit 110, liquid storage unit 120 and reaction member 130 are communicated with the atmosphere, thus
Guarantee the proper flow of liquid in the chip.For example, can use venting channels realizes that the connection with atmosphere specifically mixes
Pond 131, sample amounts separate tank 113 and distribution pipe 132 are connected to ambient atmosphere by multiple venting channels or by multiple logical
Gas channel is connected to realization with the other structures of chip 100 from ventilation.
With reference to Fig. 2, embodiment according to the present utility model, sample treatment unit 110 includes: sample feeding pipe 111, sample introduction prevention
Hole 112 and sample amounts separate tank 113, wherein sample introduction prevents hole 112 from passing through 114 phase of the first capillary pipeline with sample feeding pipe 111
Even, and sample introduction prevents the diameter in hole 112 greater than the diameter of the first capillary pipeline 114；Sample amounts separate tank 113 and sample introduction prevent
Hole 112 is connected.The diameter in sample introduction prevention hole 112 is significantly coarser than the diameter of the first capillary pipeline 114 as a result, to form interface
Valve can prevent sample excess sample introduction from entering sample amounts separate tank 113 in advance, make the quantitative more acurrate of sample.
With reference to Fig. 3, embodiment according to the present utility model, buffering sub-district 123 and quantitative sub-district 124 are logical by the first contraction
Road 126 is connected, and quantitative sub-district 124 is connected with waste liquid sub-district 125 by the second contraction channel 127, and first shrinks 126 He of channel
Second, which shrinks channel 127, is located between the centrifugation center of quantitative 124 multiple determination chip of sub-district.Wherein, " being centrifuged " center " is
Refer to when chip is carried out centrifugal treating, the center that centrifugal movement is surrounded.In centrifugal treating, the buffer of sub-district 123 is buffered
First shrinks channel 126 by quantitative sub-district 124, and when buffer is full of quantitative sub-district 124, extra buffer passes through second
Channel 127 is shunk because entering waste liquid sub-district 125 under centrifugation power drive.The main body of error formed due to chip when centrifugation is quantitative
In the height of present liquid level, the position of the first contraction channel 126 and the second contraction 127 relative quantification sub-district 124 of channel is equivalent to
The entrance on its top, top inlet is smaller, and volume change caused by liquid level is smaller.Thus first shrinks 126 He of channel
Second shrinks the smaller volumetric errors caused by advantageously reducing the error because of liquid level in cross section in channel 127, thus can be into
One step improves the quantitative accuracy of chip buffer, to further increase the accuracy in detection of chip.
Embodiment according to the present utility model, first shrink channel 126 and buffer 123 junction of sub-district area of section and
Buffer 123 average radial area of section of sub-district ratio be 0.001-0.5, wherein buffering sub-district 123 radial cross-sectional area be
Refer to perpendicular to chip 100, and by buffering sub-district towards the section in quantitative sub-district direction, first shrinks channel 126 and quantitative son
The ratio of the area of section of 124 junction of area and quantitative 124 average radial area of section of sub-district is 0.001-0.5, wherein quantitative
The radial cross-sectional area of sub-district 124 refers to perpendicular to chip 100, and by quantitative sub-district 124 towards buffering 123 direction of sub-district
Section.The area ratio of the first radial section for shrinking channel and buffering sub-district and quantitative sub-district is suitable for both guaranteeing to buffer as a result,
Liquid flows smoothly, but also the liquid height variation bring volume change of the upper end (close to centrifugation center) of quantitative sub-district compared with
It is small, make the quantitative more acurrate of quantitative sub-district.
Embodiment according to the present utility model, second shrink the area of section in channel 127 and quantitative 124 junction of sub-district and
The ratio of quantitative 124 average radial area of section of sub-district is 0.001-0.5, wherein quantitatively the radial cross-sectional area of sub-district 124 is
Refer to perpendicular to chip 100, and by quantitative sub-district 124 towards buffering 123 direction of sub-district section, second shrink channel 127 with
The ratio of the 125 average radial area of section of area of section and waste liquid sub-district of 125 junction of waste liquid sub-district is 0.001-0.5,
In, 125 radial section of waste liquid sub-district refers to perpendicular to chip 100, and by waste liquid sub-district 125 towards the section of quantitative sub-district 124.
The area ratio of the second radial section for shrinking channel and quantitative sub-district and waste liquid sub-district is suitable for both guaranteeing buffer as a result,
It is smooth to be expelled to waste liquid sub-district, but also the liquid height of the upper end (close to centrifugation center) of quantitative sub-district changes bring volume
Variation is smaller, makes the quantitative more acurrate of quantitative sub-district.
Wherein, it should be noted that " average radial area of section " forms the flat of the area of each radial section of the sub-district
Mean value.
Further, with some embodiments of the utility model, it can be packaged with dilution in advance in buffering sub-district 123
Liquid, can also buffering sub-district 123 at there are dilution sample holes, in chip in use, dilution is added by sample holes.
Specific embodiment according to the present utility model, the volume of quantitative sub-district 124 is 20 μ L-5ml, and buffers sub-district 125
Volume be not less than quantitative sub-district 124 volume half.The buffering biggish purpose of 125 volume of sub-district is for temporary receiving
The dilution of quantitative sub-district 124 will be entered, because the cross section in the first contraction channel 126 is smaller, when dilution drives in centrifugal force
When rapidly being flowed out by dilution storage area 121 under dynamic, the first contraction channel 126 of smaller cross-sectional area is possible to cannot to allow outflow
Dilution completely into quantitative sub-district 124, thus the dilution that cannot rapidly enter quantitative sub-district 124 will be temporarily stored in buffering
In sub-district 125, avoids flowing to outside other regions or chip of chip, influence chip functions.Sub-district 124 and buffering are quantified as a result,
The needs of micro-example detection needed for the volume of sub-district 125 meets conventional clinic and experiment, and ensure that sample detection
Accuracy.Further, embodiment according to the present utility model, first, which shrinks channel 126 and second, shrinks the radial direction in channel 127
Diameter is 0.1-2mm, length 0.1-2mm.The diameter in the first contraction channel and the second contraction channel is smaller as a result, so that
Liquid volume therein is smaller, conducive to the accurate quantitative analysis to buffer volume.
Further, as shown in figure 3, embodiment according to the present utility model, buffering sub-district 123 and quantitative sub-district 124
Longitudinal section is in inverted trapezoidal, wherein longitudinal section, which refers to, section of the center of chip to circumferential direction, that is to say, that buffering sub-district
123 and quantitative 124 bottom size of sub-district it is small and top dimension is big, be on the one hand conducive to demoulding when chip injection molding, on the other hand
The residual of liquid is reduced when advantageously reducing chip centrifugation discharge buffer.Because liquid can be past separate in the case where being centrifuged power drive
The direction at centrifugation center moves, so that last a small amount of liquid can converge to the upper edge for leaning near surface close to chip, and is used for
The capillary 142 of discharge liquid connecting with reaction member 130 is in the position, thus is conducive to all quantitative sub-districts
124 liquid discharge, avoids liquid from remaining.In addition, the structure of inverted trapezoidal is also avoided that dilution enters buffering sub-district 123 and determines
It bottles up when quantum area 124 gas, to influence quantitative.To sum up, the above structure can improve buffer dosing accuracy, make chip pair
The accuracy of the analysis of sample is higher.
With reference to Fig. 4, embodiment according to the present utility model, reaction member 130 includes: mixing pit 131,132 and of distribution pipe
Multiple reaction tanks 133, wherein distribution pipe 132 includes: that siphon prevents hole 134 and liquid dispensing tube 135, siphon prevent hole 134 with
Mixing pit 131 is connected by the second capillary pipeline 136, and siphon prevents the diameter in hole 134 from being greater than the straight of the second capillary pipeline 136
Diameter, liquid dispensing tube 135 prevent hole 134 from being connected with siphon, and multiple reaction tanks 133 are connected with liquid dispensing tube 135.In mixing pit
Treated, and quantitative sample is mixed with quantitative buffer, obtains mixed liquor, mixed liquor is through 136 influent of the second capillary pipeline
It during distribution pipe 135, first passes through siphon and prevents hole 134, since siphon prevents the diameter in hole 134 from being greater than the second capillary pipeline
136 diameter avoids the mixed liquor in the second capillary pipeline 136 from entering liquid dispensing tube 135 in advance and lead to part reaction tank
133 are not filled.
With reference to Fig. 4, embodiment according to the present utility model, mixing pit 131 includes: buffer area 138 and mixed zone 139,
In, buffer area 138 is connected with sample treatment unit 110；Mixed zone 139 is connected with the free end of buffer area 138, after quantitative
Sample enters third capillary pipeline 141 under the action of hydrophilic capillary force, relatively deep wider because of the presence of buffer area 138
Structure can play the role of interface valve compared to third capillary pipeline 141, so that chip is in static or sample when slowly running
Product are stopped in third capillary pipeline 141, do not enter mixing pit 131 in advance.It is fixed only when chip is centrifuged with fast speed
The sample of amount just can enter mixed zone 139 via buffer area 138 by third capillary pipeline.In addition, buffer area 138 there are energy
The length of third capillary pipeline 141 is reduced, to reduce hydrophilic third capillary pipeline 141 contamination residual possible to sample.
In addition, buffer area 138 is avoided that in mixture length third capillary pipeline 141 directly and sample and dilution in mixed zone 139
Liquid contact, the sample after avoiding diluted enter third capillary pipeline 141, thus the shadow with quantitatively remaining sample contact
It rings quantitative and pollutes.
Further, embodiment according to the present utility model, mixed zone 139 can be connected with ventilation pipe, to keep
Pressure is suitable in mixed zone 139, and liquid easily enters, further, the radial ruler of the junction of mixed zone 139 and snorkel
It is very little to change with the change of radial height, so that liquid when mixing be avoided to enter ventilation pipe and block ventilation.
Specifically, some embodiments according to the present utility model are fixed with dry Biological indicators in each reaction tank 133
Detection reagent, including glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, glutamyl transpeptidase, alkaline phosphatase, cholinesterase, phenylalanine ammonia
Based transferase, aspartate amino transferase, γ-paddy ammonia phthalidyl transferase, total protein, albumin, glycosylated albumin, total gallbladder
It is red pigment, bilirubin direct, ammonia, urea, creatinine, uric acid, glucose, total cholesterol, triglycerides, high-density lipoprotein, low close
Spend low albumen, very low density lipoprotein, Apolipoprotein A1, apolipoprotein B, calcium, chlorine, iron, phosphorus, sodium, potassium, carbon dioxide.
As shown in figure 4, embodiment according to the present utility model, reaction member 130 further comprises: reaction solution waste liquid pool
137, which is connected with distribution pipe 132, and is located remotely from the siphon and prevents to cheat 134 distribution pipe 132
End prevents hole 134 from entering distribution pipe 132, by distributing through siphon under the action of the centrifugal force through the mixed mixed liquor of mixing pit
Pipe 132 enters a reaction tank 133, and extra mixed liquor then enters reaction solution waste liquid pool 137.Have when in reaction solution waste liquid pool 137
It is when illustrating that the mixed liquor filling in each reaction tank 133 finishes, and there is no waste liquid in reaction solution waste liquid pool 137, then each anti-when waste liquid
The mixed liquor in pond 133 is answered to be possible to not complete to fill, that is, the amount of mixed liquor may be insufficient.As a result, according to reaction solution
Whether waste liquid pool 137, which has solution to enter, can determine whether reaction tank is fully filled.In addition, mixed liquor enters reaction tank 133
Afterwards, residual liquid also enters reaction solution waste liquid pool 137 in distribution pipe 132, and noresidue liquid, avoids difference in distribution pipe 132
The contact of solution between reaction tank 07, has prevented the possibility of cross contamination.
Further, embodiment according to the present utility model, the chip 100 can be rounded or fan-shaped etc. structures, pass through rotation
Core 100 flows to generate the fluid in centrifugal force driving chip 100, position of the above structure relative to the centrifugation center of chip
Relationship are as follows: near centrifugation center, it is remote that sample amounts separate tank 113 is located at sample feeding pipe 111 for sample feeding pipe 111 and buffer sublayer area 123
The side of the luxuriant heart in the heart, quantitative sub-district 124 are located at buffering side of the sub-district 123 far from centrifugation center, and mixing pit 131 is located at sample
Product Quantitative Separation pond 113 and quantitative side of the sub-district 124 far from centrifugation center, it is farthest luxuriant that each reaction tank 133 is located at chip 100
In the heart the edge of the heart and relative to centrifugation center present circumference to being uniformly distributed, distribution pipe 132 is located at reaction tank 133 and mixing pit
Between 131.For quantitative sub-district 124, sub-district 123 is buffered compared to quantitative sub-district 124 and waste liquid sub-district 125 closer in centrifugation
The heart.
Further, some embodiments according to the present utility model, the surface structure of the chip 100 can for fan-like pattern,
Diamond shape, rectangle, triangle, ellipse or circle etc..Other embodiments, the material of the chip 100 can be polymethyl
The high molecular material such as the high molecular polymers such as sour methyl esters, polycarbonate, polypropylene, or glass, metal material, Huo Zhewei
Any combination of previous materials.Wherein, it should be noted that need to be formed by transparent material at reaction tank 133.In addition, chip
100 can be sealed by the modes such as the modes such as machining, injection molding, 3D printing and hot-press sealing, laser welding, ultrasonic welding, bonding
What fixation was prepared.
As shown in figure 5, embodiment according to the present utility model, which further comprises: multiple grooves 170, multiple
Groove 170 corresponds in a thickness direction with multiple reaction tanks 133, and the back side of reaction tank 133 is arranged in.That is,
The back of each reaction tank 133 is correspondingly arranged a groove 140, and groove 140 and reaction tank 133 are oppositely arranged, to avoid core
Piece 100 pollutes when processing or use because contacting or rubbing or the optical property of 133 region of injury response pond.
The edge circumferentially of embodiment according to the present utility model, the chip 100 is serrated, and reaction tank 133 with
The recessed portion of sawtooth 138 is radially arranged in a one-to-one correspondence.When chip 100 is placed on matched centrifugation detection device,
Broached-tooth design 138 can be by optical detection devices such as the photoswitches of corollary equipment, so that trigger signal is realized to broached-tooth design 138
The detection of corresponding reaction tank 133.
Some embodiments according to the present utility model, the chip 100 also have location hole 140, specifically, the location hole
140 can be through-hole, have corresponding positioning protrusion or positioning column in analytical equipment, when positioning protrusion or positioning column protrude into and determine
In the hole 140 of position, i.e., the chip 100 is arranged in the predetermined position of analytical equipment.The location hole 140 is used for chip as a result,
100 are put into the fixation position of analytical equipment, consequently facilitating subsequent processing.
With reference to Fig. 3, some embodiments according to the present utility model, the chip 100 also has plunger valve 150, the plunger valve
150 can be through-hole structure.The plunger valve 150 setting is between buffering sub-district 123 and quantitative sub-district 124.Plunger valve 140 can quilt
Special mechanical structure on matched centrifugation apparatus opens, and realizes the flow path of dilution storage area 121 and dilution quantification area 122
Connection.
Further, as shown in figure 5, chip back is there are also contact recess 160, contact recess 180 is conducive to chip toward instrument
The contact of finger when pick-and-place on device.
The multiple determination chip in order to facilitate understanding provides the side detected using the multiple determination chip herein
Method, specific as follows:
(1) such as Fig. 6 A, the injection port of chip sample feeding pipe 111 is contacted with sample to several seconds by hydrophilic capillarity by sample
It sucks sample feeding pipe 111 or sample and chip sample feeding pipe 111 is directly added by injection port by liquid-transfering device.In liquid storage unit 120
In be preinstalled with reaction needed for liquid, such as dilution, or liquid storage unit 120 is added into before use.After completing sample introduction
Chip is placed on matched centrifugation apparatus, and plunger valve 150 can be opened by the special mechanical structure on matched centrifugation apparatus, real
The now fluid communication of buffering sub-district 123 and quantitative sub-district 124.
(2) such as Fig. 6 B, using centrifugation apparatus with higher speed rotary chip, such as 1000-6000rpm, chip sample feeding pipe
Sample in 111 prevents hole 112 from entering the complete paired samples of sample amounts separate tank 113 under the driving of centrifugal force by sample introduction
Volume quantitative and particulate matter precipitation and separation, and the liquid in dilution storage area 121 can enter dilution in the case where being centrifuged power drive
Complete dilution volume quantitative in quantification area 122.
(3) rotation speed of chip is reduced, such as 0-600rpm, the sample in sample amounts separate tank 113 is in capillary force
Effect is lower to be full of capillary 141, and the liquid in dilution quantification area 122 is full of and 131 phase of mixing pit under the action of capillary force
Even capillary 142.
(4) such as Fig. 6 C, high speed rotation chip, quantitative sample can enter mixing pit 131 by capillary again, while dilute
It releases liquid and mixing pit 131 is entered by dilution quantification area 122, then by being switched fast centrifugal direction or variation centrifugal rotational speed, realize
Sample is mixed with the volume quantitative of dilution.
(5) rotation speed of chip 100 is reduced, the liquid in mixing pit 131 is full of and second under the action of capillary force
Capillary 136.
(6) such as Fig. 6 D, high speed rotation chip again, liquid in mixing pit 131 can by the second capillary 136 via point
132 injection centrifugation of piping enters each reaction tank 133, it is filled up one by one, and extra sample enters in reaction solution waste liquid pool 137,
Complete the distribution of sample.Not residual liquid in distribution pipe 132, avoids the contact of solution between differential responses pond 133, prevents
The possibility of cross contamination.
(7) sample distributed can dissolve the reaction reagent pre-installed in reaction tank 133, carry out under certain temperature incubation biochemical
It reacts and completes to detect.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is contained at least one embodiment or example of the utility model.In the present specification, to the schematic table of above-mentioned term
Stating may not refer to the same embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be
It can be combined in any suitable manner in any one or more embodiment or examples.
While there has been shown and described that the embodiments of the present invention, it will be understood by those skilled in the art that:
These embodiments can be carried out with a variety of variations, modification, replacement in the case where not departing from the principles of the present invention and objective
And modification, the scope of the utility model are defined by the claims and their equivalents.
Claims (10)
1. a kind of multiple determination chip characterized by comprising
Sample treatment unit；
Liquid storage unit, the liquid storage unit include the dilution storage area and dilution quantification area being connected, wherein the dilution
Liquid quantification area includes that interconnected buffering sub-district, quantitative sub-district and waste liquid sub-district, buffer are passed through by the dilution storage area
The buffering sub-district enters the quantitative sub-district and is quantified, and the extra buffer enters the waste liquid sub-district；And
Reaction member, the reaction member are connected with the sample treatment unit and the quantitative sub-district,
Wherein, the sample treatment unit, the liquid storage unit and the reaction member are communicated with the atmosphere.
2. multiple determination chip according to claim 1, which is characterized in that the buffering sub-district and the quantitative sub-district
Channel is shunk by first to be connected；
The quantitative sub-district is connected with the waste liquid sub-district by the second contraction channel,
And described first shrinks channel and the second contraction channel positioned at the quantitative sub-district and the multiple determination chip
Centrifugation center between.
3. multiple determination chip according to claim 2, which is characterized in that described first shrinks channel and the buffering
The area of section of sub-district junction and it is described buffering sub-district average radial area of section ratio be 0.001-0.5, described first
It shrinks channel and the area of section for quantifying sub-district junction and the ratio for quantifying sub-district average radial area of section is
0.001-0.5,
Described second shrinks the area of section in channel and the quantitative sub-district junction and the quantitative sub-district average radial section
The ratio of area is 0.001-0.5, and described second shrinks the area of section of channel and waste liquid sub-district junction and described useless
The ratio of liquid sub-district average radial area of section is 0.001-0.5.
4. multiple determination chip according to claim 1, which is characterized in that the buffering sub-district and the quantitative sub-district
Longitudinal section be in inverted trapezoidal.
5. multiple determination chip according to claim 1, which is characterized in that the volume of the quantitative sub-district is 20 μ L-
5ml, and the volume of the buffering sub-district is not less than the half of the volume of the quantitative sub-district.
6. multiple determination chip according to claim 1, which is characterized in that the sample treatment unit includes:
Sample feeding pipe；
Sample introduction prevents hole, and the sample introduction prevents hole from being connected with the sample feeding pipe by the first capillary pipeline, and the sample introduction prevents
The diameter in hole is greater than the diameter of first capillary pipeline；And
Sample amounts separate tank, the sample amounts separate tank prevent hole from being connected with the sample introduction.
7. multiple determination chip according to claim 1, which is characterized in that the reaction member includes:
Mixing pit, the mixing pit include:
Buffer area, the buffer area are connected with the sample treatment unit by third capillary pipeline；And
Mixed zone, the mixed zone are connected with the free end of the buffer area；
Distribution pipe, the distribution pipe include:
Siphon prevents hole, and the siphon prevents hole from being connected with the mixing pit by the second capillary pipeline, and the siphon prevents
The diameter in hole is greater than the diameter of second capillary pipeline；
Liquid dispensing tube, the liquid dispensing tube prevent hole from being connected with the siphon；
Multiple reaction tanks, the multiple reaction tank are connected with the liquid dispensing tube.
8. multiple determination chip according to claim 7, which is characterized in that further comprise:
Multiple grooves, the multiple groove correspond in a thickness direction with the multiple reaction tank, and are arranged described anti-
The back side of Ying Chi.
9. multiple determination chip according to claim 7, which is characterized in that the edge tool circumferentially of the chip
There is recessed portion, and the reaction tank is radially arranged in a one-to-one correspondence with the recessed portion.
10. multiple determination chip according to claim 7, which is characterized in that the reaction member further comprises:
Reaction solution waste liquid pool, the reaction solution waste liquid pool are connected with the distribution pipe, and are located remotely from the siphon and prevent hole
The distribution pipe end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820733141.8U CN208270589U (en) | 2018-05-16 | 2018-05-16 | Multiple determination chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820733141.8U CN208270589U (en) | 2018-05-16 | 2018-05-16 | Multiple determination chip |
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| Publication Number | Publication Date |
|---|---|
| CN208270589U true CN208270589U (en) | 2018-12-21 |
Family
ID=64682734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820733141.8U Active CN208270589U (en) | 2018-05-16 | 2018-05-16 | Multiple determination chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490199A (en) * | 2018-05-16 | 2018-09-04 | 清华大学 | Multiple determination chip |
| CN112986148A (en) * | 2019-12-12 | 2021-06-18 | 杭州微策生物技术股份有限公司 | Biological fluid detection device, detection system and detection method |
-
2018
- 2018-05-16 CN CN201820733141.8U patent/CN208270589U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490199A (en) * | 2018-05-16 | 2018-09-04 | 清华大学 | Multiple determination chip |
| CN112986148A (en) * | 2019-12-12 | 2021-06-18 | 杭州微策生物技术股份有限公司 | Biological fluid detection device, detection system and detection method |
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