CN110465338A - Promote the chip of fluid mixing and promotes the method for fluid mixing - Google Patents

Promote the chip of fluid mixing and promotes the method for fluid mixing Download PDF

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Publication number
CN110465338A
CN110465338A CN201810448714.7A CN201810448714A CN110465338A CN 110465338 A CN110465338 A CN 110465338A CN 201810448714 A CN201810448714 A CN 201810448714A CN 110465338 A CN110465338 A CN 110465338A
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CN
China
Prior art keywords
chip
mixed
mixing
sample
cell
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Pending
Application number
CN201810448714.7A
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Chinese (zh)
Inventor
孙冰
姜慧芸
王林
姜杰
文松
徐伟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Petroleum and Chemical Corp
China Petrochemical Corp
Sinopec Qingdao Safety Engineering Institute
Original Assignee
China Petrochemical Corp
Sinopec Qingdao Safety Engineering Institute
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Application filed by China Petrochemical Corp, Sinopec Qingdao Safety Engineering Institute filed Critical China Petrochemical Corp
Priority to CN201810448714.7A priority Critical patent/CN110465338A/en
Publication of CN110465338A publication Critical patent/CN110465338A/en
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3045—Micromixers using turbulence on microscale
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract

The present invention relates to micro-total analysis chip fields, disclose a kind of chip of promotion fluid mixing and the method for promoting fluid mixing.A kind of chip promoting fluid mixing, which includes the injection port (5) being sequentially communicated, premixing pool (1), mixed zone (2) and detection cell (3)；Wherein, the mixed zone (2) includes 2 or more mixed cells, and the mixed cell is for making sample form local turbulence in the mixed zone (2).Chip of the invention has unique mixing plot structure, so that can be sufficiently mixed during sample flows in the chips.

Description

Promote the chip of fluid mixing and promotes the method for fluid mixing
Technical field
The present invention relates to micro-total analysis chip fields, and in particular to a kind of chip for promoting fluid mixing and promotes fluid mixed The method of conjunction.
Background technique
With economic rapid development, China's water resource pollution problem is on the rise.Industrial wastewater is to influence water environment The biggest factor, carrying out prevention and treatment to industrial wastewater pollution is to influence the grand strategy of the sustainable development of national economy.So I State is always all using prevention and treatment industrial pollution as the most important thing of environmental protection.Water quality detection is the necessary previous step administered, only By the concrete condition for accurately knowing that water body forms, can just suit the remedy to the case using corresponding administering method.Meanwhile water quality detection is also Environment superintendent office judges sewage discharge the only resource whether up to standard.Exploitation is for the fast and accurately live of Complex water body Detection technique is imperative.
During detecting using microfluidic chip analysis, it is frequently necessary to realize the equal of two kinds of fluids on micro-fluidic chip Even mixing, and due to the needs of micro-fluidic chip size and operation, it is desirable to make two kinds of fluids in the time as short as possible and to the greatest extent may be used It can small being uniformly distributed spatially.Since fluid Reynolds number is low in microchannel, had been disclosed at present using moulds such as micro-valve, Micropumps Block additionaling power source promotes mixed method, but the above method must introduce special construction or operation.And do not have outside certain Add under the occasion of power source (that is, there is no the modules such as micro-valve, Micropump), how to need to realize by chip structure passive mixed It closes, or realizes quickly mixing using simple manual operation, still without preferable solution.
Summary of the invention
The purpose of the invention is to overcome the above problem of the existing technology, a kind of core of promotion fluid mixing is provided Piece and the method for promoting fluid mixing, which has unique mixing plot structure, so that the process that sample flows in the chips In can be sufficiently mixed.
To achieve the goals above, one aspect of the present invention provide it is a kind of promotion fluid mixing chip, the chip include according to Injection port, premixing pool, mixed zone and the detection cell of secondary connection；Wherein, the mixed zone includes 2 or more mixed cells, should Mixed cell is for making sample form local turbulence in the mixed zone.
Preferably, the mixed zone includes 2-50 mixed cell
Preferably, the chip is circle, and the injection port is located at the center of circle of the chip；The premixing pool, mixed zone It is sequentially distributed with detection cell along the radial direction of the chip.
Preferably, the mixed cell includes mixing pit and the mixed column that is arranged in the mixing pit, and the mixing is singly Member along the chip radial direction axisymmetricly, the mixed column flows through sample from the two sides of the mixed column and in institute Converge the other side for stating mixed column.
Preferably, the mixed cell includes curved flow passage structure.
Preferably, the sectional area of the runner constantly changes, and reaches minimum in knee.
Preferably, the chip further includes positioned at the annular runner close to the chip periphery, the annular runner and institute State detection cell connection.
Preferably, the mixed cell is distributed around the center of circle of the chip；The mixed cell includes two or more and shunts Runner and mixing pit, sample are passing through the flow manifold into mixing pit mixing respectively.
Second aspect of the present invention provides a kind of method of promotion fluid mixing, this method comprises: passing sequentially through sample Injection port, premixing pool, mixed zone and the detection cell of chip of the invention are stated, and is mixed in mixed zone.
Preferably, this method promotes fluid mixing by carrying out centrifugation to the chip.
Third aspect present invention provides the chip for promoting fluid mixing of aforementioned present invention or the side for promoting fluid to mix Application of the method in environmental pollution analyte detection or in-vitro diagnosis field.
Through the above technical solutions, by the present invention in that chip has unique mixing plot structure, so that sample is in chip Turbulent flow is generated during middle flowing, completes quickly, adequately to mix, especially suitable for realizing plurality of reagents on chip Mixing in situ.Since the flowing of chip upper fluid is based on laminar flow, in no special designing, mixing relies primarily on diffusion and completes, effect Rate is low；After introducing mixing plot structure, can easily and fast, efficiently it be realized using the power of script driving liquid flowing more The mixing of kind liquid.In addition, being arranged mixed zone in pre-mix zone downstream, can be completed directly in pre-mix zone after being simply mixed (dissolution of such as solid) is thoroughly mixed in mixed zone immediately.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the chip of specific embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the chip of another specific embodiment of the invention；
Fig. 3 is the structural schematic diagram of the mixed zone of different structure of the invention.
Description of symbols
1, premixing pool 2, mixed zone 3, detection cell
4, annular runner 5, injection port
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more New numberical range, these numberical ranges should be considered as specific open herein.
The chip provided by the invention for promoting fluid mixing, the chip include the injection port 5 being sequentially communicated, premixing pool 1, mix Close area 2 and detection cell 3；Wherein, the mixed zone 2 includes 2 or more mixed cells, and the mixed cell is for making sample in institute It states mixed zone 2 and forms local turbulence.
In chip of the invention, local turbulence is being formed by sample, the sample not being uniformly mixed can be enable It is enough to be uniformly mixed so that the operations such as detection of subsequent sample are more accurate and convenient.
In the present invention, it by including multiple mixed cells in the mixed zone 2, can sufficiently be carried out in mixed zone 2 Mixed process, the preferably described mixed zone 2 include 2-50 mixed cell, such as can be 2-20,3-8,3-5 etc..
A preferred embodiment according to the present invention, the chip are circle, and the injection port is located at the chip The center of circle；The premixing pool 1, mixed zone 2 and detection cell 3 are sequentially distributed along the radial direction of the chip.By by chip It is provided in round, mixed process of the centrifugation driving sample in chip of the invention, easy to operate, mixing efficiency can be passed through It is high.When using above-mentioned circular chip structure, premixing pool 1, mixed zone 2 and the detection cell 3 of preferably different groups are on the chip It is uniformly distributed, i.e., the angle between different groups of premixing pools 1, mixed zone 2 and detection cell 3 is equal.
As the method for making sample form local turbulence in the mixed zone 2, one according to the present invention preferred implementation Mode, the mixed cell include mixing pit and the mixed column that is arranged in the mixing pit, and the mixed cell is along the core Axisymmetricly, the mixed column flows through sample from the two sides of the mixed column and in the mixed column radial direction of piece Converge the other side.It is preferred that this has the chip use of mixed column in above-mentioned circular chip.As above-mentioned mixed column, shape There is no particular limitation with quantity, can be more than one, such as can be 2,3,4 or 5 etc., shape can for linear, Fold-line-shaped, shuttle shape etc., as long as perpendicular to fluid original flow direction.As shown in the A in Fig. 1 and Fig. 3, the mixing pit is five Side shape, the mixed column be fold-line-shaped, thus make sample must be flowed through from the two sides of the mixed column when flowing out mixing pit and The other side of mixed column is converged, to achieve the purpose that mix sample.As shown in the C and D in Fig. 3, the mixed column is 3, One of them (mixed column I) is positioned close to the side of mixing pit entrance, and another two (mixed column II and III) is symmetricly set on and leans on The side of nearly mixing pit outlet, sample are first flowed around the two sides mixed column I after entering mixing pit, then rotating around mixed column II and III flowing, finally converges before flowing out from mixing pit.
As the shape of mixed column, there is no particular limitation, as long as can achieve the purpose that above-mentioned adjusting sample flow process .Such as can be round, ellipse or polygon, from the standpoint of efficiency for adjusting sample flow, it is however preferred to have wedge angle Shape.Region between the mixed column and mixing pit for sample flow is preferably smaller, such as accounts for mixing pool area 60% hereinafter, it is preferred that 50% or less, 40% hereinafter, to forcing sample to mix；It is described from the point of view of being flowed through convenient for sample Between mixed column and mixing pit for sample flow region can for example account for mix 10% of pool area or more, 20 or more or 30% or more.The mixed column can be the bulge-structure being arranged on the mixing pit bottom surface, or upper end with it is described The column structure that the top surface of mixing pit connects, wherein preferably the latter.
As the method for making sample form local turbulence in the mixed zone 2, such as sample can be made in the mixed zone 2 Change flow velocity.Another preferred embodiment according to the present invention, the mixed cell include curved flow passage structure.Pass through It is flowed in curved runner, so that the flowing velocity of sample constantly changes, to be located between the sample of fluid different zones It is mixed.There is no particular limitation for above-mentioned curved mode, can be arc (such as Fig. 2), or fold-line-shaped (such as Fig. 3 In B).
Above-mentioned curved runner can have uniform sectional area (such as Fig. 2), it is possible to have continually changing sectional area (B in such as Fig. 3).
A preferred embodiment according to the present invention, as shown in the B in Fig. 3, the sectional area of the runner constantly becomes Change, and reaches minimum in knee.Change collective effect by the bending of runner and sectional area, sample can be made utmostly Ground carries out flow velocity change in mixed zone 2, to improve mixing efficiency.
According to the present invention, in the case that the chip is annular, it is preferable that the chip further includes being located at close to the core The annular runner 4 of piece periphery, the annular runner are connected to the detection cell 3.By the way that above-mentioned annular runner 4 is arranged, can make The pressure balance between different groups of premixing pool 1, mixed zone 2 and detection cell 3 is obtained, convenient for mixing.
Another preferred embodiment according to the present invention, the mixed cell are distributed around the center of circle of the chip；Institute Stating mixed cell includes two or more flow manifolds and mixing pit, and sample is entering the mixing by the flow manifold respectively Pond mixing.As shown in the E in Fig. 3, each mixed cell include two flow manifolds and circular mixing pit, typical two points One is linear in stream runner, another is arc, and wherein the flow manifold of arc is positioned close to the edge of chip.Make Method to drive said chip preferably makes chip be rotated (such as carrying out by centrifugation) around its center of circle, to make sample Two flow manifolds are respectively enterd from mixing pit, then are mixed in next mixing pit, by the speed of control rotation, so that on The process of stating repeats, the sample that the liquid outlet of final chip is uniformly mixed.
The present invention also provides a kind of methods of promotion fluid mixing, this method comprises: sample is made to pass sequentially through above-mentioned Injection port 5, premixing pool 1, mixed zone 2 and the detection cell 3 of the chip of invention, and mixed in mixed zone 2.Core through the invention The unique chip structure of piece, it is ensured that sample is sufficiently mixed in mixed zone.
According to the present invention, as the method for mixing sample in chip of the invention, such as can be by adding to sample Pressure makes it flow successively through injection port 5, premixing pool 1, mixed zone 2 and the completion of detection cell 3, can also be made by carrying out centrifugation to chip Sample flows successively through injection port 5, premixing pool 1, mixed zone 2 and detection cell 3 and completes.From the point of view of easy to operate, it is preferable that This method promotes fluid mixing by carrying out centrifugation to the chip.
It in the present invention, can be the sample for passing through injection port 5 and being added in chip, or pre- as above-mentioned sample Set sample in the chips.Sample can be more than one, such as two kinds, three kinds, four kinds, can occur between sample or not It is mutually dissolved, reacts, by using the chip and mixed method of aforementioned present invention, it is sufficiently mixed to be finally reached various samples The purpose of conjunction.Specifically, can to need various solids or fluid sample to be used in chip detection process, such as water sample, Humoral sample, reaction reagent etc..
The present invention also provides a kind of water quality many reference amounts detection devices, which includes aforementioned present invention Promote fluid mixing chip.
In the present invention, which can also include more than one detection part.
According to the present invention, there is no particular limitation for the detection part, can be used for the various detection parts of chip detection, Such as the detection part can be chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman signal inspection Survey one of component and infrared spectroscopy detection part or a variety of.It is wherein preferably absorbance detection component and/or fluorescence detection Component.
For the ease of the progress of detection, the chip can be fixed on the water quality in order to any way of detection joins more It measures in detection device, such as can fix, rotatable or slidable mode.
A preferred embodiment according to the present invention, the detection part is by light source, branch optical fiber and spectroscopy equipment It constitutes.The light source is used for for generating the light for carrying out testing required wavelength, the branch optical fiber while detecting multiple reaction tanks Interior optical signalling, the spectroscopy equipment are used to collect optical signal generating or after absorbing.When needing to different location When reaction tank is detected, the incident light that the branch optical fiber can will test is divided into multichannel, so that detection be rapidly completed.
According to the present invention, in order to realize that function is integrated, which can also include signal acquisition part One of part, data processor, data output component and data communication component are a variety of.It is detected as the water quality many reference amounts A kind of preferred embodiment of equipment comprising data processor, data output component, data transfer component.As described Data processor can be the central processing unit etc. including controller, arithmetic unit and register；As the data output section Part may include having display screen, touch screen, voice output of images outputting function etc.；It, can as the data transfer component It is remotely handled so that initial data is uploaded to remote server, then result is returned directly into designated terminal, can also be incited somebody to action Data processor generates treated information remote transmitting to cloud and carries out information collection.
In the present invention, which can be used for sample introduction component, the sample introduction component to described Chip sample introduction.The existing various sample injectors commonly used in chip sample introduction can be used in the sample introduction component, can for manually or Person is automatic, as needed, has been can be set on the sample introduction component for carrying out pretreated pretreatment portion, or control to sample The scale etc. of sample volume processed.
The present invention also provides the chip of above-mentioned promotion fluid mixing or promote the method for fluid mixing in environmental pollution The application in analyte detection or in-vitro diagnosis field.
The present invention will be described in detail by way of examples below.It, will be purchased from Chinese standard substance in following embodiment The 080220 100mg/L ammonia and nitrogen components analytical standard substance of GBW (E) of net flows through after diluting 10 times is prefixed powdered ammonia nitrogen inspection Enter reading pond 3 altogether after the premixing pool 1 of test agent (Japan vertical physics and chemistry water-quality test packet WAK- ammonia nitrogen) and mixed zone 2, immediately into The test of row absorbance.Mixing efficiency is investigated by testing mixed absorbance change.It is tested by spectrophotometry, it is real Testing equipment is ocean optics company deuterium lamp/tungsten light source and QE Pro spectrometer, Detection wavelength 640nm.
Embodiment 1
It is detected using chip as shown in Figure 1.
The chip is circle, and injection port 5 is arranged in its center of circle, which includes eight groups of injection ports being sequentially communicated 5, premix Pond 1, mixed zone 2 and detection cell 3, and positioned at the annular runner 4 close to the chip periphery, the annular runner and the inspection Pond 3 is surveyed to be connected to.The premixing pool 1 is rectangle, and detection cell 3 is circle, and mixed zone 2 includes two mixed cells, and each mixing is single Include into the mixed column of pentagonal mixing pit and fold-line-shaped in member.
Ammonia nitrogen detection reagent powder is preset in premixing pool 1.
Standard solution is injected by injection port 5 and makes it into premixing pool 1, is pre-mixed with above-mentioned preset reagent.With it is upper Sample after stating preset reagent premixing enters two mixed cells of mixed zone 2, and the mixed column mixes sample from described The two sides of zygostyle are flowed through and are converged in the other side of the mixed column, to complete the mixing of sample Yu preset reagent.Sample is logical After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using fiber spectrometer, result such as the following table 1.Wherein detect Position a, b, c respectively represent the different location of detection cell, and wherein a is located at close to detection cell entrance, and it is central that b is located at detection cell, and c In the opposite side far from detection cell entrance, position of the optical path by detection cell, detection needed for each position are changed by moving fiber Time is about 5s, and the reaction time is about 8 minutes, and successively test detected the absorbance from position a, b, c since the 8th minute.
Table 1
Detection time Detect position Absorbance
8min a 0.937
8min+10s b 0.937
8min+20s c 0.937
Embodiment 2
Sample detection is carried out according to the method for embodiment 1, unlike, it is carried out using chip shown in Fig. 2.The chip with Chip difference shown in FIG. 1 is only that mixed zone 2, which includes two mixed cells, and mixed cell is curved stream Road, runner have uniform width.
Water sample is injected by injection port 5 and makes it into premixing pool 1, is pre-mixed with above-mentioned preset reagent.Sample is mixed It closes and is flowed through in the curved channel in area, due to the blocking of runner, flow velocity constantly changes, to be thoroughly mixed.Sample passes through mixed zone 2 After mixing, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as the following table 2.
Table 2
Detection time Detect position Absorbance
8min a 0.938
8min+10s b 0.939
8min+20s c 0.938
Embodiment 3
Sample detection is carried out according to the method for embodiment 1, unlike, the mixed zone 2 of chip has such as the D institute in Fig. 3 The structure shown.Mixed zone 2 includes 8 mixed cells, and each mixed cell includes irregular polygon mixing pit and 3 mixing Column, one (mixed column I) for rectangle and is positioned close to the side of mixing pit entrance, another two (mixed column in 3 mixed columns II and III) be approximately triangle and setting be symmetricly set on close to mixing pit outlet side, sample enter mixing pit after, It first flows around the two sides mixed column I, then is flowed rotating around mixed column II and III, finally converged before being flowed out from mixing pit.Sample is logical After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as following table 3。
Table 3
Detection time Detect position Absorbance
8min a 0.937
8min+10s b 0.938
8min+20s c 0.938
Embodiment 4
Sample detection is carried out according to the method for embodiment 1, unlike, chip is circle, and mixed zone 2 has such as Fig. 3 In E shown in structure.Mixed zone 2 includes the mixed cell that 6 centers of circle around the chip are distributed, and each mixed cell includes Two flow manifolds and mixing pit, sample are passing through the flow manifold into mixing pit mixing respectively.Sample passes through mixed After closing the mixing of area 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as the following table 4.
Table 4
Detection time Detect position Absorbance
8min a 0.937
8min+10s b 0.938
8min+20s c 0.938
Comparative example 1
Sample detection is carried out according to the method for embodiment 1, unlike, do not have mixed column in mixed cell.Sample is logical After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as following table 5。
Table 5
Detection time Detect position Absorbance
8min a 0.814
8min+10s b 0.822
8min+20s c 0.837
By the testing result of comparative example 1 and comparative example 1 it is found that different detection position a of detection cell 3 in embodiment 1, B, the absorbance of c is essentially identical, illustrates that the mixed cell with mixed column has reached good mixed effect, can guarantee detection cell The absorbance uniformity of sample to be tested in 3；And in comparative example 1 absorbance of different detection position a, b, the c of detection cell 3 have it is larger Difference illustrates that mixed effect is poor, cannot complete uniformly to mix.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to Protection scope of the present invention.

Claims (11)

1. a kind of chip for promoting fluid mixing, which is characterized in that the chip includes the injection port (5) being sequentially communicated, premixing pool (1), mixed zone (2) and detection cell (3)；
Wherein, the mixed zone (2) includes 2 or more mixed cells, and the mixed cell is for making sample in the mixed zone (2) local turbulence is formed.
2. chip according to claim 1, wherein the mixed zone (2) includes 2-50 mixed cell.
3. chip according to claim 1, wherein the chip is circle, and the injection port is located at the circle of the chip The heart；
The premixing pool (1), mixed zone (2) and detection cell (3) are sequentially distributed along the radial direction of the chip.
4. chip described in any one of -3 according to claim 1, wherein the mixed cell includes that mixing pit and setting exist Mixed column in the mixing pit,
The mixed cell along the chip radial direction axisymmetricly,
The mixed column makes sample flow through from the two sides of the mixed column and converge in the other side of the mixed column.
5. chip described in any one of -3 according to claim 1, wherein the mixed cell includes curved runner knot Structure.
6. chip according to claim 5, wherein the sectional area of the runner constantly changes, and reaches most in knee It is small.
7. chip described in any one of -6 according to claim 1, wherein the chip further includes being located at close to the chip The annular runner (4) of periphery, the annular runner are connected to the detection cell (3).
8. chip according to claim 1, wherein the mixed cell is distributed around the center of circle of the chip；
The mixed cell includes two or more flow manifolds and mixing pit, and sample is entering institute by the flow manifold respectively State mixing pit mixing.
9. a kind of method for promoting fluid mixing, which is characterized in that this method comprises: sample is made to pass sequentially through claim 1-8 Any one of described in chip injection port (5), premixing pool (1), mixed zone (2) and detection cell (3), and in mixed zone (2) It is mixed.
10. according to the method described in claim 9, wherein, this method promotes fluid to mix by carrying out centrifugation to the chip.
11. the side of promotion fluid mixing described in chip described in any one of claim 1-8 or claim 9 or 10 Application of the method in environmental pollution analyte detection or in-vitro diagnosis field.
CN201810448714.7A 2018-05-11 2018-05-11 Promote the chip of fluid mixing and promotes the method for fluid mixing Pending CN110465338A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114534814A (en) * 2022-03-29 2022-05-27 天津诺迈科技有限公司 Micro-fluidic chip
CN114887536A (en) * 2022-05-18 2022-08-12 西安交通大学 High-flux passive mixing device and method with short mixing distance

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004294417A (en) * 2003-03-26 2004-10-21 Yasuhiro Horiike Micro mixer, sample analyzing kit, and manufacturing method therefor
CN101817495A (en) * 2010-03-25 2010-09-01 湖南大学 Micro fluid control chip and preparation method and application thereof
CN103230753A (en) * 2013-04-10 2013-08-07 中国科学院电工研究所 Micro-mixing detecting chip
CN105214546A (en) * 2015-09-22 2016-01-06 北京工业大学 A kind of concussion jetting type micro-mixer based on Pulsating Flow
CN106215985A (en) * 2016-07-26 2016-12-14 西安交通大学 A kind of micro-fluidic chip quickly mixing for fluid and detecting
CN107398307A (en) * 2016-05-18 2017-11-28 博奥生物集团有限公司 A kind of integrated micro-flow control chip
CN208356803U (en) * 2018-05-11 2019-01-11 中国石油化工股份有限公司 Promote the chip and water quality many reference amounts detection device of fluid mixing

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004294417A (en) * 2003-03-26 2004-10-21 Yasuhiro Horiike Micro mixer, sample analyzing kit, and manufacturing method therefor
CN101817495A (en) * 2010-03-25 2010-09-01 湖南大学 Micro fluid control chip and preparation method and application thereof
CN103230753A (en) * 2013-04-10 2013-08-07 中国科学院电工研究所 Micro-mixing detecting chip
CN105214546A (en) * 2015-09-22 2016-01-06 北京工业大学 A kind of concussion jetting type micro-mixer based on Pulsating Flow
CN107398307A (en) * 2016-05-18 2017-11-28 博奥生物集团有限公司 A kind of integrated micro-flow control chip
CN106215985A (en) * 2016-07-26 2016-12-14 西安交通大学 A kind of micro-fluidic chip quickly mixing for fluid and detecting
CN208356803U (en) * 2018-05-11 2019-01-11 中国石油化工股份有限公司 Promote the chip and water quality many reference amounts detection device of fluid mixing

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114534814A (en) * 2022-03-29 2022-05-27 天津诺迈科技有限公司 Micro-fluidic chip
CN114887536A (en) * 2022-05-18 2022-08-12 西安交通大学 High-flux passive mixing device and method with short mixing distance

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