CN208356803U - Promote the chip and water quality many reference amounts detection device of fluid mixing - Google Patents
Promote the chip and water quality many reference amounts detection device of fluid mixing Download PDFInfo
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- CN208356803U CN208356803U CN201820700168.7U CN201820700168U CN208356803U CN 208356803 U CN208356803 U CN 208356803U CN 201820700168 U CN201820700168 U CN 201820700168U CN 208356803 U CN208356803 U CN 208356803U
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Abstract
The utility model relates to micro-total analysis chip fields, disclose the water quality many reference amounts detection device of a kind of chip that promotion fluid mixes and the chip for promoting fluid mixing including the utility model.The chip of the utility model includes the injection port (5) being sequentially communicated, premixing pool (1), mixed zone (2) and detection cell (3)；Wherein, the mixed zone (2) includes 2 or more mixed cells, and the mixed cell is for making sample form local turbulence in the mixed zone (2).The chip of the utility model has unique mixing plot structure, so that can be sufficiently mixed during sample flows in the chips.
Description
Technical field
The utility model relates to micro-total analysis chip fields, and in particular to it is a kind of promote fluid mixing chip and water quality it is more
Parametric Detection equipment.
Background technique
With economic rapid development, China's water resource pollution problem is on the rise.Industrial wastewater is to influence water environment
The biggest factor, carrying out prevention and treatment to industrial wastewater pollution is to influence the grand strategy of the sustainable development of national economy.So I
State is always all using prevention and treatment industrial pollution as the most important thing of environmental protection.Water quality detection is the necessary previous step administered, only
By the concrete condition for accurately knowing that water body forms, can just suit the remedy to the case using corresponding administering method.Meanwhile water quality detection is also
Environment superintendent office judges sewage discharge the only resource whether up to standard.Exploitation is for the fast and accurately live of Complex water body
Detection technique is imperative.
During detecting using microfluidic chip analysis, it is frequently necessary to realize the equal of two kinds of fluids on micro-fluidic chip
Even mixing, and due to the needs of micro-fluidic chip size and operation, it is desirable to make two kinds of fluids in the time as short as possible and to the greatest extent may be used
It can small being uniformly distributed spatially.Since fluid Reynolds number is low in microchannel, had been disclosed at present using moulds such as micro-valve, Micropumps
Block additionaling power source promotes mixed method, but the above method must introduce special construction or operation.And do not have outside certain
Add under the occasion of power source (that is, there is no the modules such as micro-valve, Micropump), how to need to realize by chip structure passive mixed
It closes, or realizes quickly mixing using simple manual operation, still without preferable solution.
Utility model content
The purpose of this utility model is to overcome the above problems of the existing technology, provide a kind of promotion fluid mixing
Chip and water quality many reference amounts detection device, the chip have unique mixing plot structure so that sample flowed in the chips
It can be sufficiently mixed in the process.
To achieve the goals above, on the one hand the utility model provides the chip of a kind of promotion fluid mixing, the chip packet
Include the injection port being sequentially communicated, premixing pool, mixed zone and detection cell；Wherein, the mixed zone includes 2 or more mixing lists
Member, the mixed cell is for making sample form local turbulence in the mixed zone.
Preferably, the mixed zone includes 2-50 mixed cell
Preferably, the chip is circle, and the injection port is located at the center of circle of the chip；The premixing pool, mixed zone
It is sequentially distributed with detection cell along the radial direction of the chip.
Preferably, the mixed cell includes mixing pit and the mixed column that is arranged in the mixing pit, and the mixing is singly
Member along the chip radial direction axisymmetricly, the mixed column flows through sample from the two sides of the mixed column and in institute
Converge the other side for stating mixed column.
Preferably, the mixed cell includes curved flow passage structure.
Preferably, the sectional area of the runner constantly changes, and reaches minimum in knee.
Preferably, the chip further includes positioned at the annular runner close to the chip periphery, the annular runner and institute
State detection cell connection.
Preferably, the mixed cell is distributed around the center of circle of the chip；The mixed cell includes two or more and shunts
Runner and mixing pit, sample are passing through the flow manifold into mixing pit mixing respectively.
The utility model second aspect provides a kind of water quality many reference amounts detection device, which includes
The chip for promoting fluid mixing of aforementioned present invention.
Preferably, which further includes more than one detection part；The detection part is coloration
In detection part, absorbance detection component, fluorescence signal detection part, Raman signal detection component and infrared spectroscopy detection part
It is one or more.
Preferably, the water quality many reference amounts detection device further include drive chip rotation driving part, signal acquisition component,
One of data processor, data output component and data transfer component are a variety of.
Through the above technical solutions, the utility model by make chip have unique mixing plot structure so that sample exists
Turbulent flow is generated during flowing in chip, completes quickly, adequately to mix, especially suitable for realizing a variety of examinations on chip
The mixing in situ of agent.Since the flowing of chip upper fluid is based on laminar flow, in no special designing, mixing, which is relied primarily on, has spread
At low efficiency；After introducing and mixing plot structure, using script driving liquid flowing power can easily and fast, it is efficiently real
The mixing of existing plurality of liquid.In addition, being arranged mixed zone in pre-mix zone downstream, can complete to be simply mixed directly in pre-mix zone
(dissolution of such as solid) is thoroughly mixed in mixed zone immediately afterwards.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the chip of specific embodiment of the utility model；
Fig. 2 is the structural schematic diagram of the chip of the utility model another kind specific embodiment；
Fig. 3 is the structural schematic diagram of the mixed zone of the different structure of the utility model.
Description of symbols
1, premixing pool 2, mixed zone 3, detection cell
4, annular runner 5, injection port
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The chip provided by the utility model for promoting fluid mixing, which includes the injection port 5 being sequentially communicated, premixing pool
1, mixed zone 2 and detection cell 3；Wherein, the mixed zone 2 includes 2 or more mixed cells, and the mixed cell is for making sample
Local turbulence is formed in the mixed zone 2.
In the chip of the utility model, local turbulence is being formed by sample, the sample not being uniformly mixed can be made
Product can be uniformly mixed so that the operations such as the detection of subsequent sample are more accurate and convenient.
It in the present invention, can be abundant in mixed zone 2 by including multiple mixed cells in the mixed zone 2
Mixed process is carried out, the preferably described mixed zone 2 includes 2-50 mixed cell, such as can be 2-20,3-8,3-5
Deng.
A preferred embodiment according to the present utility model, the chip are circle, and the injection port is located at described
The center of circle of chip；The premixing pool 1, mixed zone 2 and detection cell 3 are sequentially distributed along the radial direction of the chip.Passing through will
Chip is provided in round, and mixed process of the sample in the chip of the utility model can be driven by centrifugation, easy to operate, is mixed
It closes high-efficient.When using above-mentioned circular chip structure, premixing pool 1, mixed zone 2 and the detection cell 3 of preferably different groups are described
It is uniformly distributed on chip, i.e., the angle between different groups of premixing pools 1, mixed zone 2 and detection cell 3 is equal.
As the method for making sample form local turbulence in the mixed zone 2, one according to the present utility model preferred
Embodiment, the mixed cell include mixing pit and the mixed column that is arranged in the mixing pit, and the mixed cell is along institute
State the radial direction of chip axisymmetricly, the mixed column flows through sample from the two sides of the mixed column and in the mixing
Converge the other side of column.It is preferred that this has the chip use of mixed column in above-mentioned circular chip.As above-mentioned mixed column,
There is no particular limitation for shape and quantity, can be more than one, such as can be 2,3,4 or 5 etc., and shape can be straight line
Shape, fold-line-shaped, shuttle shape etc., as long as perpendicular to fluid original flow direction.As shown in the A in Fig. 1 and Fig. 3, the mixing pit
For pentagon, the mixed column is fold-line-shaped, to make sample that must flow through from the two sides of the mixed column when flowing out mixing pit
And converge in the other side of mixed column, to achieve the purpose that mix sample.As shown in the C and D in Fig. 3, the mixed column is 3
A, one of them (mixed column I) is positioned close to the side of mixing pit entrance, and another two (mixed column II and III) is symmetrical arranged
In the side close to mixing pit outlet, sample is first flowed around the two sides mixed column I after entering mixing pit, then rotating around mixed column
II and III flowing, finally converges before flowing out from mixing pit.
As the shape of mixed column, there is no particular limitation, as long as can achieve the purpose that above-mentioned adjusting sample flow process
?.Such as can be round, ellipse or polygon, from the standpoint of efficiency for adjusting sample flow, it is however preferred to have wedge angle
Shape.Region between the mixed column and mixing pit for sample flow is preferably smaller, such as accounts for mixing pool area
60% hereinafter, it is preferred that 50% or less, 40% hereinafter, to forcing sample to mix；It is described from the point of view of being flowed through convenient for sample
Between mixed column and mixing pit for sample flow region can for example account for mix 10% of pool area or more, 20 or more or
30% or more.The mixed column can be the bulge-structure being arranged on the mixing pit bottom surface, or upper end with it is described
The column structure that the top surface of mixing pit connects, wherein preferably the latter.
As the method for making sample form local turbulence in the mixed zone 2, such as sample can be made in the mixed zone 2
Change flow velocity.Another preferred embodiment according to the present utility model, the mixed cell include curved flow passage structure.
By being flowed in curved runner, so that the flowing velocity of sample constantly changes, to be located at the sample of fluid different zones
Between mixed.There is no particular limitation for above-mentioned curved mode, can be arc (such as Fig. 2), or fold-line-shaped is (such as
B in Fig. 3).
Above-mentioned curved runner can have uniform sectional area (such as Fig. 2), it is possible to have continually changing sectional area
(B in such as Fig. 3).
A preferred embodiment according to the present utility model, as shown in the B in Fig. 3, the sectional area of the runner is not
Disconnected variation, and reach minimum in knee.Change collective effect by the bending of runner and sectional area, sample can be made maximum
Flow velocity change is carried out to degree in mixed zone 2, to improve mixing efficiency.
According to the utility model, in the case that the chip is annular, it is preferable that the chip further includes being located at close to institute
The annular runner 4 of chip periphery is stated, the annular runner is connected to the detection cell 3.It, can by the way that above-mentioned annular runner 4 is arranged
So that the pressure balance between the premixing pool 1 of different groups, mixed zone 2 and detection cell 3, convenient for mixing.
Another preferred embodiment according to the present utility model, the mixed cell is around the center of circle of the chip point
Cloth；The mixed cell includes two or more flow manifolds and mixing pit, and sample is entering institute by the flow manifold respectively
State mixing pit mixing.As shown in the E in Fig. 3, each mixed cell includes two flow manifolds and circular mixing pit, typically
One is linear in two flow manifolds, another is arc, and wherein the flow manifold of arc is positioned close to the side of chip
At edge.As the method for driving said chip, chip is preferably made to be rotated (such as carrying out by centrifugation) around its center of circle, thus
So that sample is respectively enterd two flow manifolds from mixing pit, then is mixed in next mixing pit, the speed rotated by control,
So that the above process repeats, the sample that the liquid outlet of final chip is uniformly mixed.
The utility model additionally provides a kind of method of promotion fluid mixing, this method comprises: passing sequentially through sample
Injection port 5, premixing pool 1, mixed zone 2 and the detection cell 3 of the chip of the utility model are stated, and is mixed in mixed zone 2.Pass through
The unique chip structure of the utility model chip, it is ensured that sample is sufficiently mixed in mixed zone.
According to the utility model, as the method for mixing sample in the chip of the utility model, such as can pass through
Make that it flows successively through injection port 5, premixing pool 1, mixed zone 2 and detection cell 3 are completed to sample pressurization, can also by chip into
Row centrifugation makes sample flow successively through injection port 5, premixing pool 1, mixed zone 2 and the completion of detection cell 3.It is examined from angle easy to operate
Consider, it is preferable that this method promotes fluid to mix by carrying out centrifugation to the chip.
In the present invention, can be for the sample in chip be added by injection port 5 as above-mentioned sample, it can also be with
For preset sample in the chips.Sample can be more than one, such as two kinds, three kinds, four kinds, can occur between sample or
Person is not mutually dissolved, reacts, and by using the chip and mixed method of above-mentioned the utility model, is finally reached a variety of samples
The well-mixed purpose of product.It specifically, can be to need various solids or fluid sample to be used, example in chip detection process
Such as water sample, humoral sample, reaction reagent.
The utility model also provides a kind of water quality many reference amounts detection device, which includes above-mentioned
The chip for promoting fluid mixing of utility model.
In the present invention, which can also include more than one detection part.
According to the utility model, there is no particular limitation for the detection part, can be used for the various detections of chip detection
Component, such as the detection part can be chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman
One of signal detecting part and infrared spectroscopy detection part are a variety of.It is wherein preferably absorbance detection component and/or glimmering
Photodetector.
For the ease of the progress of detection, the chip can be fixed on the water quality in order to any way of detection joins more
It measures in detection device, such as can fix, rotatable or slidable mode.
A preferred embodiment according to the present utility model, the detection part is by light source, branch optical fiber and spectrum
Equipment is constituted.The light source is used for while detecting multiple anti-for generating the light for carrying out testing required wavelength, the branch optical fiber
Optical signalling in Ying Chi, the spectroscopy equipment are used to collect optical signal generating or after absorbing.When needs are to different positions
When the reaction tank set is detected, the incident light that the branch optical fiber can will test is divided into multichannel, so that detection be rapidly completed.
According to the utility model, in order to realize that function is integrated, which can also adopt including signal
Collect one of component, data processor, data output component and data communication component or a variety of.As the water quality many reference amounts
A kind of preferred embodiment of detection device comprising data processor, data output component, data transfer component.As
The data processor can be the central processing unit etc. including controller, arithmetic unit and register；It is defeated as the data
Component out may include having display screen, touch screen, voice output of images outputting function etc.；As the data transfer part
Initial data can be uploaded to remote server and remotely be handled, then result is returned directly to designated terminal by part, can also
Data processor is generated that treated, information remote transmitting to cloud carries out information collection.
In the present invention, the water quality many reference amounts detection device can with sample introduction component, the sample introduction component be used for
The chip sample introduction.The existing various sample injectors commonly used in chip sample introduction can be used in the sample introduction component, can be hand
It is dynamic or automatic, as needed, can be set on the sample introduction component for carrying out pretreated pretreatment portion to sample, or
The scale etc. of person's control sample volume.
The chip or water quality many reference amounts detection device of the above-mentioned promotion fluid mixing of the utility model can be in environment dirts
It is applied in dye analyte detection or in-vitro diagnosis field.
The utility model will be described in detail by embodiment below.It, will be purchased from Chinese standard in following embodiment
The 080220 100mg/L ammonia and nitrogen components analytical standard substance of GBW (E) of substance net flows through after diluting 10 times and is prefixed powdered ammonia
Enter after the premixing pool 1 of nitrogen detection reagent (physics and chemistry water-quality test packet WAK- ammonia nitrogen founds altogether in Japan) and mixed zone 2 and read pond 3, stands
Carry out absorbance test.Mixing efficiency is investigated by testing mixed absorbance change.It is surveyed by spectrophotometry
Examination, experimental facilities are ocean optics company deuterium lamp/tungsten light source and QE Pro spectrometer, Detection wavelength 640nm.
Embodiment 1
It is detected using chip as shown in Figure 1.
The chip is circle, and injection port 5 is arranged in its center of circle, which includes eight groups of injection ports being sequentially communicated 5, premix
Pond 1, mixed zone 2 and detection cell 3, and positioned at the annular runner 4 close to the chip periphery, the annular runner and the inspection
Pond 3 is surveyed to be connected to.The premixing pool 1 is rectangle, and detection cell 3 is circle, and mixed zone 2 includes two mixed cells, and each mixing is single
Include into the mixed column of pentagonal mixing pit and fold-line-shaped in member.
Ammonia nitrogen detection reagent powder is preset in premixing pool 1.
Standard solution is injected by injection port 5 and makes it into premixing pool 1, is pre-mixed with above-mentioned preset reagent.With it is upper
Sample after stating preset reagent premixing enters two mixed cells of mixed zone 2, and the mixed column mixes sample from described
The two sides of zygostyle are flowed through and are converged in the other side of the mixed column, to complete the mixing of sample Yu preset reagent.Sample is logical
After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using fiber spectrometer, result such as the following table 1.Wherein detect
Position a, b, c respectively represent the different location of detection cell, and wherein a is located at close to detection cell entrance, and it is central that b is located at detection cell, and c
In the opposite side far from detection cell entrance, position of the optical path by detection cell, detection needed for each position are changed by moving fiber
Time is about 5s, and the reaction time is about 8 minutes, and successively test detected the absorbance from position a, b, c since the 8th minute.
Table 1
| Detection time | Detect position | Absorbance |
| 8min | a | 0.937 |
| 8min+10s | b | 0.937 |
| 8min+20s | c | 0.937 |
Embodiment 2
Sample detection is carried out according to the method for embodiment 1, unlike, it is carried out using chip shown in Fig. 2.The chip with
Chip difference shown in FIG. 1 is only that mixed zone 2, which includes two mixed cells, and mixed cell is curved stream
Road, runner have uniform width.
Water sample is injected by injection port 5 and makes it into premixing pool 1, is pre-mixed with above-mentioned preset reagent.Sample is mixed
It closes and is flowed through in the curved channel in area, due to the blocking of runner, flow velocity constantly changes, to be thoroughly mixed.Sample passes through mixed zone 2
After mixing, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as the following table 2.
Table 2
| Detection time | Detect position | Absorbance |
| 8min | a | 0.938 |
| 8min+10s | b | 0.939 |
| 8min+20s | c | 0.938 |
Embodiment 3
Sample detection is carried out according to the method for embodiment 1, unlike, the mixed zone 2 of chip has such as the D institute in Fig. 3
The structure shown.Mixed zone 2 includes 8 mixed cells, and each mixed cell includes irregular polygon mixing pit and 3 mixing
Column, one (mixed column I) for rectangle and is positioned close to the side of mixing pit entrance, another two (mixed column in 3 mixed columns
II and III) be approximately triangle and setting be symmetricly set on close to mixing pit outlet side, sample enter mixing pit after,
It first flows around the two sides mixed column I, then is flowed rotating around mixed column II and III, finally converged before being flowed out from mixing pit.Sample is logical
After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as following table
3。
Table 3
| Detection time | Detect position | Absorbance |
| 8min | a | 0.937 |
| 8min+10s | b | 0.938 |
| 8min+20s | c | 0.938 |
Embodiment 4
Sample detection is carried out according to the method for embodiment 1, unlike, chip is circle, and mixed zone 2 has such as Fig. 3
In E shown in structure.Mixed zone 2 includes the mixed cell that 6 centers of circle around the chip are distributed, and each mixed cell includes
Two flow manifolds and mixing pit, sample are passing through the flow manifold into mixing pit mixing respectively.Sample passes through mixed
After closing the mixing of area 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as the following table 4.
Table 4
| Detection time | Detect position | Absorbance |
| 8min | a | 0.937 |
| 8min+10s | b | 0.938 |
| 8min+20s | c | 0.938 |
Comparative example 1
Sample detection is carried out according to the method for embodiment 1, unlike, do not have mixed column in mixed cell.Sample is logical
After crossing the mixing of mixed zone 2, detection cell 3 is eventually entered into, is detected using method same as Example 1, result such as following table
5。
Table 5
| Detection time | Detect position | Absorbance |
| 8min | a | 0.814 |
| 8min+10s | b | 0.822 |
| 8min+20s | c | 0.837 |
By the testing result of comparative example 1 and comparative example 1 it is found that different detection position a of detection cell 3 in embodiment 1,
B, the absorbance of c is essentially identical, illustrates that the mixed cell with mixed column has reached good mixed effect, can guarantee detection cell
The absorbance uniformity of sample to be tested in 3；And in comparative example 1 absorbance of different detection position a, b, the c of detection cell 3 have it is larger
Difference illustrates that mixed effect is poor, cannot complete uniformly to mix.
Preferred embodiments of the present invention are described in detail above, still, the utility model is not limited to this.At this
In the range of the technology design of utility model, a variety of simple variants can be carried out to the technical solution of the utility model, including each
Technical characteristic is combined with any other suitable method, these simple variants and combination equally should be considered as the utility model
Disclosure of that belongs to the protection scope of the utility model.
Claims (11)
1. a kind of chip for promoting fluid mixing, which is characterized in that the chip includes the injection port (5) being sequentially communicated, premixing pool
(1), mixed zone (2) and detection cell (3)；
Wherein, the mixed zone (2) includes 2 or more mixed cells, and the mixed cell is for making sample in the mixed zone
(2) local turbulence is formed.
2. chip according to claim 1, which is characterized in that the mixed zone (2) includes 2-50 mixed cell.
3. chip according to claim 1, which is characterized in that the chip is circle, and the injection port is located at the core
The center of circle of piece；
The premixing pool (1), mixed zone (2) and detection cell (3) are sequentially distributed along the radial direction of the chip.
4. chip described in any one of -3 according to claim 1, which is characterized in that the mixed cell include mixing pit and
Mixed column in the mixing pit is set,
The mixed cell along the chip radial direction axisymmetricly,
The mixed column makes sample flow through from the two sides of the mixed column and converge in the other side of the mixed column.
5. chip described in any one of -3 according to claim 1, which is characterized in that the mixed cell includes curved stream
Road structure.
6. chip according to claim 5, wherein the sectional area of the runner constantly changes, and reaches most in knee
It is small.
7. chip described in any one of -3 according to claim 1, which is characterized in that the chip further includes being located at close to institute
The annular runner (4) of chip periphery is stated, the annular runner is connected to the detection cell (3).
8. chip according to claim 1, which is characterized in that the mixed cell is distributed around the center of circle of the chip；
The mixed cell includes two or more flow manifolds and mixing pit, and sample is entering institute by the flow manifold respectively
State mixing pit mixing.
9. a kind of water quality many reference amounts detection device, which is characterized in that the water quality many reference amounts detection device includes in claim 1-8
The chip of promotion fluid mixing described in any one.
10. water quality many reference amounts detection device according to claim 9, which is characterized in that the water quality many reference amounts detection device
It further include more than one detection part；
The detection part is chromaticity sensing section, absorbance detection component, fluorescence signal detection part, Raman signal detection portion
One of part and infrared spectroscopy detection part are a variety of.
11. water quality many reference amounts detection device according to claim 9 or 10, which is characterized in that water quality many reference amounts detection
Equipment further includes driving driving part, signal acquisition component, data processor, data output component and the data of chip rotation
One of transmission part is a variety of.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110465338A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Promote the chip of fluid mixing and promotes the method for fluid mixing |
| CN110470610A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Water quality many reference amounts detection device and detection method |
| CN114534814A (en) * | 2022-03-29 | 2022-05-27 | 天津诺迈科技有限公司 | Micro-fluidic chip |
-
2018
- 2018-05-11 CN CN201820700168.7U patent/CN208356803U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110465338A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Promote the chip of fluid mixing and promotes the method for fluid mixing |
| CN110470610A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Water quality many reference amounts detection device and detection method |
| CN114534814A (en) * | 2022-03-29 | 2022-05-27 | 天津诺迈科技有限公司 | Micro-fluidic chip |
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