CN106554902B - A kind of power-chemical Coupling biology experimental installation - Google Patents
A kind of power-chemical Coupling biology experimental installation Download PDFInfo
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- CN106554902B CN106554902B CN201611016545.7A CN201611016545A CN106554902B CN 106554902 B CN106554902 B CN 106554902B CN 201611016545 A CN201611016545 A CN 201611016545A CN 106554902 B CN106554902 B CN 106554902B
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The present invention relates to biology techniques fields, and in particular to a kind of power-chemical Coupling biology experimental installation, it includes experimental provision ontology, which includes working plate, and the bottom surface quadrangle of the working plate is provided with studdle foot；The left and right sides is correspondingly arranged on left through-hole and right through-hole on the working plate, and left bulge loop and right bulge loop are respectively arranged in left through-hole and right through-hole, and experiment package and control component are respectively arranged on left bulge loop and right bulge loop；The experiment package includes experiment box, which is made of back box A, box cover A and transparent experimental cell cup；It has structure simple, at low cost, using compareing and testing two sets of parallel elements, shortens experimental period, improves the accuracy of experimental result and improve work efficiency.
Description
[technical field]
The present invention relates to biology techniques fields, and in particular to a kind of power-chemical Coupling biology experimental installation.
[background technique]
Cell biology is to study structure, a science of function and various life rule of cell.Be with cell be grind
Study carefully object, with dynamic viewpoint, studies the structure and function of cell and crganelle, the history of life of cell and various vital movements rule
The subject of rule.Cell biology is one of Front component subject of modern life science, mainly from the different structure layer of cell
The secondary basic law to study the vital movement of cell.In terms of life layer of structure, cell biology be located at molecular biology with
It between Developmental Biology, is mutually linked, interpenetrates with them.Cell it is extremely complex in body environment, by mechanics, chemistry etc.
The separately or cooperatively effect of many factors, in order to which preferably observation-chemical coupling effect is to vascular endothelial cell, tumour cell
Deng effect phenomenon, and further investigate relevant molecule mechanism, need to design and a set of can guarantee experimental group and control group research object
By identical power-electrochemical conditions stimulation, traditional power-chemical stimulation device design scheme is solved.Patent of invention CN101201348: one
The mechanical and biological experiments device of mechanics and chemistry signals is planted while providing, there are easy-to-leak liquid, film load is difficult, and device precision is not
Height, experimental result inaccuracy the problems such as.
[summary of the invention]
In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of structure is simple, design rationally, make
With convenient power-chemical Coupling biology experimental installation,
It is experiment package and control component respectively using two sets of components are provided on workbench；It has structure
Simply, at low cost, using compareing and testing two sets of parallel elements, shorten experimental period, while overcoming easy-to-leak liquid, film load is tired
Difficulty, device precision is not high, the problem of experimental result inaccuracy, improves the accuracy of experimental result and improves work effect
Rate.
A kind of power-chemical Coupling biology experimental installation of the present invention, it includes experimental provision ontology, experiment dress
Setting ontology includes working plate, and the bottom surface quadrangle of the working plate is provided with studdle foot；
The left and right sides is correspondingly arranged on left through-hole and right through-hole on the working plate, sets respectively in left through-hole and right through-hole
It is equipped with left bulge loop and right bulge loop, experiment package and control component are respectively arranged on left bulge loop and right bulge loop；
The experiment package includes experiment box, which is made of back box A, box cover A and transparent experimental cell cup；Back box
It is provided with box cover A on A, transparent experimental cell cup is provided in back box A, is provided with biomembrane A in transparent experimental cell cup；It is described
Inlet pipe A is provided on box cover A；
The control component includes comparison box, which is made of back box B, box cover B and transparent comparison cell cup；Back box
It is provided with box cover B on B, transparent comparison cell cup is provided in back box B, is provided with biomembrane B in transparent comparison cell cup；It is described
Inlet pipe B is provided on box cover B；It is provided with conduit A between the inlet pipe A, inlet pipe B, is provided with the chemical stimulation factor on conduit A
Supplement bottle；
The working plate is connect with bottom plate；There is vallecular cavity on the bottom plate；The bottom plate two sides are correspondingly arranged on into outflow
Flow tube mouth is provided with conduit B between two inflow outflow nozzles, is provided with peristaltic pump on conduit B.
Further, the vallecular cavity is rectangle, is provided with rectangular seal on the notch of the vallecular cavity.
After adopting the above structure, the invention has the following beneficial effects: a kind of power-chemical Coupling biological experiment of the present invention
Device, it is experiment package and control component respectively using two sets of components are provided on workbench；It has structure letter
It is single, it is at low cost, using compareing and testing two sets of parallel elements, shorten experimental period, while overcoming easy-to-leak liquid, film load is tired
Difficulty, device precision is not high, the problem of experimental result inaccuracy, improves the accuracy of experimental result and improves work effect
Rate.
[Detailed description of the invention]
Described herein the drawings are intended to provide a further understanding of the invention, constitutes part of this application, but
It does not constitute improper limitations of the present invention, in the accompanying drawings:
Fig. 1 is schematic structural view of the invention；
Fig. 2 is the structural schematic diagram that the box cover A of experiment box is opened in the present invention；
Fig. 3 is the overlooking structure diagram of the working plate in Fig. 1；
Fig. 4 is the structural schematic diagram of Fig. 1 insole board；
Fig. 5 is the structure schematic diagram of Fig. 1 insole board.
Description of symbols:
1, experimental provision ontology；2, working plate；21, Zuo Tongkong；22, right through-hole；23, left bulge loop；24, right bulge loop；3,
Studdle foot；4, inflow outflow nozzle；5, conduit B；6, peristaltic pump；7, bottom plate；8, box is tested；81, back box A；82, box cover A；
83, inlet pipe A；84, transparent experimental cell cup；9, box is compared；91, back box B；92, box cover B；93, inlet pipe B；10, conduit A；11, change
It learns stimulating factor and supplements bottle；12, rectangular seal；13, vallecular cavity.
[specific embodiment]
Come that the present invention will be described in detail below in conjunction with attached drawing and specific embodiment, illustrative examples therein and says
It is bright to be only used to explain the present invention but not as a limitation of the invention.
As Figure 1-Figure 5, a kind of power-chemical Coupling biology experimental installation described in present embodiment, it is wrapped
Experimental provision ontology 1 is included, which includes working plate 2, and the bottom surface quadrangle of the working plate 2 is provided with support
Screw rod foot 3；
The left and right sides is correspondingly arranged on left through-hole 21 and right through-hole 22, Zuo Tongkong 21 and right through-hole on the working plate 2
It is respectively arranged with left bulge loop 23 and right bulge loop 24 in 22, is respectively arranged with experiment package and control on left bulge loop 23 and right bulge loop 24
Component；The experiment package includes experiment box 8, and the experiment box 8 is by back box A81, box cover A82 and 84 groups of transparent experimental cell cup
At；It is provided with box cover A82 on back box A81, is provided with transparent experimental cell cup 84 in back box A81, in transparent experimental cell cup 84
It is provided with biomembrane A；Inlet pipe A83 is provided on the box cover A82；
The control component includes comparison box 9, and the comparison box 9 is by back box B91, box cover B92 and transparent comparison cell cup group
At；It is provided with box cover B92 on back box B91, transparent comparison cell cup, setting in transparent comparison cell cup are provided in back box B91
There is biomembrane B；Inlet pipe B93 is provided on the box cover B；It is provided with conduit A10 between the inlet pipe A83, inlet pipe B93, this is led
Chemical stimulation factor supplement bottle 11 is provided on pipe A10；
The working plate 2 is connect with bottom plate 7；There is vallecular cavity 13 on the bottom plate 7；7 two sides of bottom plate are correspondingly arranged on
Inflow outflow nozzle 4 is provided with conduit B5 between two inflow outflow nozzles 4, peristaltic pump 6 is provided on conduit B5.
Preferably as one kind of the invention, the vallecular cavity is rectangle, is provided with rectangular seal 12 on the notch of the vallecular cavity.
In the present invention, experiment package and control component is respectively set in the left and right sides on working plate 2, by using above-mentioned
Two groups of parallel devices shorten experimental period, overcome biomembrane load difficulty, device precision is not high, and parallel laboratory test result is inaccurate
True problem can significantly improve the accuracy of experimental result, improve working efficiency.
In the present invention, it is provided with vallecular cavity on bottom plate 7, is sealed using rectangular seal 12, so that rectangular seal cavity is formed,
Then power is provided by peristaltic pump, load fluid applies fluid shear stress stimulation to transparent cup floor cells.
In the present invention, conduit A10, conduit B5 form circulation pipe, and chemical stimulation factor supplement bottle is provided on conduit A10
11, peristaltic pump 6 provides power, applies fluid shear stress stimulation to transparent cup floor cells by load fluid.This place it is transparent
Cup refers to transparent experimental cell cup 84 and transparent comparison cell cup.Using experiment package and control component to convenient in same item
Under part, research is compared, and is able to solve asking for original power-chemical stimulation easy leakage of loading device design scheme simultaneously
Topic.
A kind of power-chemical Coupling biology experimental installation of the present invention, it uses and is provided with two on workbench
Component is covered, is experiment package and control component respectively；It has structure simple, at low cost, parallel with two sets of experiment using control
Component shortens experimental period, while overcoming easy-to-leak liquid, and film load is difficult, and device precision is not high, and experimental result inaccuracy is asked
Topic, improves the accuracy of experimental result and improves work efficiency.
The above description is only a preferred embodiment of the present invention, thus it is all according to the configuration described in the scope of the patent application of the present invention,
The equivalent change or modification that feature and principle are done, is included in the scope of the patent application of the present invention.
Claims (2)
1. a kind of power-chemical Coupling biology experimental installation, it is characterised in that: it includes experimental provision ontology, the experimental provision
Ontology includes working plate, and the bottom surface quadrangle of the working plate is provided with studdle foot；The left and right sides on the working plate
It is correspondingly arranged on left through-hole and right through-hole, left bulge loop and right bulge loop, left bulge loop and the right side are respectively arranged in left through-hole and right through-hole
Experiment package and control component are respectively arranged on bulge loop；The experiment package includes experiment box, and the experiment box is by back box A, box
Cover A and transparent experimental cell cup composition；It is provided with box cover A on back box A, transparent experimental cell cup, transparent reality are provided in back box A
It tests in cell cup and is provided with biomembrane A；Inlet pipe A is provided on the box cover A；The control component includes comparison box, the comparison
Box is made of back box B, box cover B and transparent comparison cell cup；It is provided with box cover B on back box B, transparent comparison is provided in back box B
Cell cup is provided with biomembrane B in transparent comparison cell cup；Inlet pipe B is provided on the box cover B；The inlet pipe A, inlet pipe B it
Between be provided with conduit A, be provided on the conduit A chemical stimulation factor supplement bottle；The working plate is connect with bottom plate；The bottom
There is vallecular cavity on plate；The bottom plate two sides are correspondingly arranged on inflow outflow nozzle, are provided with conduit between two inflow outflow nozzles
Peristaltic pump is provided on B, conduit B.
2. a kind of power-chemical Coupling biology experimental installation according to claim 1, it is characterised in that: the vallecular cavity is
Rectangle is provided with rectangular seal on the notch of the vallecular cavity.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2016108004974 | 2016-09-02 | ||
| CN201610800497 | 2016-09-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106554902A CN106554902A (en) | 2017-04-05 |
| CN106554902B true CN106554902B (en) | 2019-03-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611016545.7A Active CN106554902B (en) | 2016-09-02 | 2016-11-18 | A kind of power-chemical Coupling biology experimental installation |
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| CN (1) | CN106554902B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101201348A (en) * | 2007-11-22 | 2008-06-18 | 四川大学 | Mechanics biology experimental installation capable of providing mechanics and chemistry signals synchronously |
| CN101942389A (en) * | 2010-09-27 | 2011-01-12 | 北京航空航天大学 | Mechanical loading flow chamber device for co-culturing in-vitro cells |
| CN102443534A (en) * | 2011-10-14 | 2012-05-09 | 天津开发区合普工贸有限公司 | Cell intermittent hypoxic simulation experiment equipment |
| CN102586084A (en) * | 2012-03-16 | 2012-07-18 | 大连理工大学 | Micro flow control shearing device for controlling two dynamic biochemical signals to fast switch stimulation in flow method |
| CN103146574A (en) * | 2011-12-07 | 2013-06-12 | 国家纳米科学中心 | High-flux microfluidic biomechanical long-term stimulation system and use thereof |
| CN105199955A (en) * | 2015-10-22 | 2015-12-30 | 重庆大学 | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading |
| CN106119079A (en) * | 2016-07-06 | 2016-11-16 | 四川大学 | Clearance flow cyto-mechanics biology experimental installation between one |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITPI20040046A1 (en) * | 2004-06-18 | 2004-09-18 | Univ Pisa | BIOREACTOR FOR THE STUDY OF THE EFFECTS ON THE CELL ACTIVITIES OF IMPOSED STIMULES |
| FR2878035B1 (en) * | 2004-11-15 | 2014-10-17 | Oreal | DEVICE COMPRISING A RECONSTRUCTED SKIN SAMPLE AND A DETECTION SYSTEM. |
-
2016
- 2016-11-18 CN CN201611016545.7A patent/CN106554902B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101201348A (en) * | 2007-11-22 | 2008-06-18 | 四川大学 | Mechanics biology experimental installation capable of providing mechanics and chemistry signals synchronously |
| CN101942389A (en) * | 2010-09-27 | 2011-01-12 | 北京航空航天大学 | Mechanical loading flow chamber device for co-culturing in-vitro cells |
| CN102443534A (en) * | 2011-10-14 | 2012-05-09 | 天津开发区合普工贸有限公司 | Cell intermittent hypoxic simulation experiment equipment |
| CN103146574A (en) * | 2011-12-07 | 2013-06-12 | 国家纳米科学中心 | High-flux microfluidic biomechanical long-term stimulation system and use thereof |
| CN102586084A (en) * | 2012-03-16 | 2012-07-18 | 大连理工大学 | Micro flow control shearing device for controlling two dynamic biochemical signals to fast switch stimulation in flow method |
| CN105199955A (en) * | 2015-10-22 | 2015-12-30 | 重庆大学 | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading |
| CN106119079A (en) * | 2016-07-06 | 2016-11-16 | 四川大学 | Clearance flow cyto-mechanics biology experimental installation between one |
Non-Patent Citations (1)
| Title |
|---|
| 力-化学耦合作用在血管内皮细胞迁移中的作用及其力学生物学机制;黄先亮等;《医用生物力学》;20101031;第25卷(第5期);第321-327页 |
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| CN106554902A (en) | 2017-04-05 |
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