CN106544423B

CN106544423B - Lodging-resistant molecular marker and application of polymorphism thereof in identification of lodging-resistant character of corn

Info

Publication number: CN106544423B
Application number: CN201610929888.6A
Authority: CN
Inventors: 林中伟; 魏莱; 张旋; 张志海; 刘欢欢
Original assignee: China Agricultural University
Current assignee: China Agricultural University
Priority date: 2016-10-31
Filing date: 2016-10-31
Publication date: 2020-03-20
Anticipated expiration: 2036-10-31
Also published as: CN106544423A

Abstract

The invention discloses an anti-lodging molecular marker and application of polymorphism thereof in identifying corn anti-lodging traits. In the application disclosed by the invention, the lodging-resistant molecular marker is a Br2 gene or a subbr 2 gene, or a DNA molecule obtained by taking the genomic DNA of corn as a template and amplifying A1 by adopting a primer; a1 consists of the single-stranded DNA shown at positions 6336-6355 of SEQ ID NO. 1 and the single-stranded DNA reverse-complementary to the single-stranded DNA shown at positions 6648-6667 of SEQ ID NO. 1. Experiments prove that the lodging resistance character of the corn can be identified by using the lodging resistance molecular marker of the invention: compared with the BB genotype of which both chromosomes contain the Br2 gene, the plant height, the height of the female ear, the height of the emasculated ear and the relative ear height of the BB genotype corn of which both chromosomes contain the subr2 gene are obviously reduced.

Description

Lodging-resistant molecular marker and application of polymorphism thereof in identification of lodging-resistant character of corn

Technical Field

The invention relates to an anti-lodging molecular marker in the field of biotechnology and application of polymorphism thereof in identification of maize anti-lodging traits.

Background

In the last 50 and 60 centuries, the phenomenon of high-stalk lodging of crops such as wheat and rice due to fertilizer application was increasingly serious, and further improvement of crop yield was restricted. In order to solve the problem, breeders respectively improve corresponding crops by utilizing semi-dwarf genes such as sd1 in rice and rht1 in wheat, and the like, so that the cultivated short-stalk variety effectively solves the lodging problem, greatly improves the yield per unit of the crops, and further initiates a first green revolution. However, the plant height is mostly controlled by qtl consisting of small polygenes, so the efficiency of breeding semi-dwarf varieties in maize is still low, and the selected genes are reduced in plant height and simultaneously reduced in female ears. The maize Dwarf genes reported so far include the Dwarf3(D3) gene encoding cytochrome P450 involved in the early biosynthetic process of gibberellins, the Anther ear1(An1) gene encoding enzymes involved in the kaurene synthetic pathway, and the Br2 gene encoding the P-glycoprotein that regulates auxin transport in maize shoots. Although the allelic diversity of corn is very rich, no gene which can effectively reduce the plant height and has little influence on the yield traits like sd1 gene in rice and rht1 gene in wheat is found at present.

Disclosure of Invention

The invention aims to solve the technical problem of how to identify the lodging resistance character of corn.

In order to solve the technical problems, the invention firstly provides the application of the lodging-resistant molecular marker or the polymorphism thereof in identifying the lodging-resistant character of the corn;

the lodging-resistant molecular marker is a DNA molecule obtained by taking the genome DNA of the corn as a template and amplifying the A1 by adopting a primer pair;

the A1 consists of single-stranded DNA named as P1 and P2, the P1 is the single-stranded DNA which is specifically combined with the 6377 th upstream position of the sequence 1 in corn genomic DNA, and the P2 is m1) or m 2): m1) and the 6617 th downstream of the sequence 1 in the corn genome DNA; m2) and the 6632 downstream position of the sequence 1 in the corn genome DNA.

In the application, the lodging resistance of the corn can be embodied in plant height, ear position height of the female ear, emasculation plant height and/or ear position relative height of the female ear.

In the application, the relative ear height of the female ear is the ratio of the ear height of the female ear to the plant height.

In the above application, the lodging-resistant molecular marker is obtained based on whether the maize genome contains the 6377-6617 th site of the sequence 1. The lodging resistance of the maize containing the 6377-6617 th site of the sequence 1 is lower than that of the maize not containing the 6377-6617 th site of the sequence 1, specifically: the height of the maize containing the 6377-6617 th site of the sequence 1 is lower than that of the maize not containing the 6377-6617 th site of the sequence 1; the ear position of the maize containing the 6377-6617 th site of the sequence 1 is lower than that of the maize not containing the 6377-6617 th site of the sequence 1; the emasculation height of the maize containing the 6377-6617 th site of the sequence 1 is lower than that of the maize not containing the 6377-6617 th site of the sequence 1; the relative ear height of the female ear of the maize containing the 6377-6617 th position of the sequence 1 is smaller than that of the maize without the 6377-6617 th position of the sequence 1.

In the application, the lodging-resistant molecule is marked as a DNA molecule shown in the 6336-6667 th position of the sequence 1 or the 6328-6427 th position of the sequence 2;

the P1 is the single-stranded DNA shown in the 6336-6355 th position of the sequence 1, and the P2 is the single-stranded DNA reverse-complementary to the single-stranded DNA shown in the 6648-6667 th position of the sequence 1.

In the above application, the lodging-resistant molecular marker may be n1) or n 2): n1) Br2 gene or subr2 gene; the sequence of the Br2 gene is shown as 1 st-6655 th of a sequence 1 in a sequence table, and the sequence of the subr2 gene is shown as 1 st-6415 th of the sequence 1 in the sequence table;

n2) the DNA molecule shown in the sequence 1 or the DNA molecule shown in the sequence 2.

In order to solve the technical problems, the invention also provides a method for identifying the corn genotype.

In the method for identifying the maize genotype, the genotypes are a BB genotype, a Bb genotype and a BB genotype, and the method comprises the following steps of I or II or III or VI:

detecting a DNA fragment corresponding to a sequence 1 in a sequence table in a corn genome DNA to be detected, wherein the corn to be detected is a BB genotype if two chromosomes in the corn genome to be detected are g 1); if the two chromosomes in the genome of the maize to be detected are the chromosomes of g2), the maize to be detected is of the bb genotype; if one of the two chromosomes in the maize genome to be detected is the chromosome of the g1) and the other chromosome is the chromosome of the g2), the maize to be detected is the Bb genotype;

g1) the DNA fragment corresponding to the sequence 1 comprises the DNA fragment shown in the 6377-th-6617 site of the sequence 1;

g2) the DNA fragment corresponding to the sequence 1 does not contain the DNA fragment shown in the 6377-6617 th site of the sequence 1;

II, detecting a DNA fragment corresponding to the 6377-6632-th site of the sequence 1 in the sequence table in the to-be-detected corn genome DNA, wherein the to-be-detected corn is a BB genotype if two chromosomes in the to-be-detected corn genome are h 1); if the two chromosomes in the genome of the maize to be detected are both the chromosomes of h2), the maize to be detected is the bb genotype; if one of the two chromosomes in the maize genome to be detected is the chromosome of h1) below and the other chromosome is the chromosome of h2) below, the maize to be detected is the Bb genotype;

h1) the 6377-6632 bit corresponding to the sequence 1 in the sequence table is the 6377-6632 bit of the sequence 1;

h2) the 6377-6632 bit corresponding to the sequence 1 in the sequence table is the 6369-6392 bit of the sequence 2;

iii, as follows, K1) and K2):

K1) performing PCR amplification by using corn genome DNA to be detected as a template and adopting the A1 to obtain a PCR product;

K2) detecting the PCR product obtained in the step K1), and determining the corn genotype according to the PCR product:

the PCR product contains A1 and does not contain A2, and the genotype of the corn to be detected is a BB genotype; the PCR product contains the A1 and the A2, and the genotype of the corn to be detected is Bb genotype; the PCR product contains the A2 and does not contain the A1, and the genotype of the corn to be detected is bb genotype;

the A1 is a DNA molecule containing a1, and the A2 is a DNA molecule not containing the a 1; the a1 is a DNA fragment shown in 6377-6617 th site of the sequence 1 or any fragment thereof;

VI, including L1) and L2) as follows:

l1) taking corn genome DNA to be detected as a template, and carrying out PCR amplification by adopting the A1 to obtain a PCR product;

l2) the following L21) or L22):

l21) detecting the size of the PCR product obtained in the step L1), and determining the corn genotype according to the size of the PCR product:

the genotype of the corn to be detected, of which the PCR product contains DNA fragments of 332bp and 100bp, is Bb genotype; the genotype of the corn to be detected, of which the PCR product contains a DNA fragment of 332bp and does not contain a DNA fragment of 100bp, is a BB genotype; the PCR product does not contain a DNA fragment of 332bp and the genotype of the corn to be detected containing the DNA fragment of 100bp is the bb genotype;

l22) detecting the sequence of the PCR product obtained in step L1), determining the maize genotype from the PCR product:

the genotype of the to-be-detected corn, of which the PCR product contains the DNA fragment shown in the 6336-6667 th site of the sequence 1 and the DNA fragment shown in the 6328-6427 th site of the sequence 2, is Bb genotype; the PCR product contains a DNA fragment shown in the 6328-6427 position of the sequence 2 and a DNA fragment shown in the 6336-6667 position of the sequence 1, and the genotype of the corn to be detected is the bb genotype; the PCR product contains a DNA fragment shown in the 6336-6667 th site of the sequence 1 and a DNA fragment shown in the 6328-6427 th site of the sequence 2, and the genotype of the corn to be detected is the BB genotype.

In the above method, the PCR product obtained in the detecting step K1) may be the size or sequence of the PCR product, and the determining the maize genotype according to the PCR product may be the determining according to the size or sequence of the PCR product.

In the above method, the concentrations of both the P1 and the P2 in the reaction system for PCR amplification using the A1 were 0.2 pmol/. mu.L. The reaction system may contain PCR buffer, dNTPs, DNA polymerase and/or water. Wherein, the PCR Buffer solution can be specifically 2 XGC Buffer I of TaKaRa. The dNTPs may be dNTPmix of TaKaRa. The DNA polymerase can be TaKaRa LA Taq of TaKaRa.

In the above method, the annealing temperature for PCR amplification using the A1 may be 55 ℃. The annealing conditions for PCR amplification using the A1 described above may be 55 ℃ for 30 s. The denaturation, annealing and extension conditions for PCR amplification using the A1 described can be 94 ℃ for 30s, 55 ℃ for 30s, 72 ℃ for 2 min. The number of cycles of denaturation, annealing and extension for PCR amplification using the a1 described can be 35. Before PCR amplification by the A1, the condition 1 can be added, and the condition 1 is denaturation, annealing and extension'. The number of cycles of condition 1 may be 1-10, such as 5. The annealing ' temperature may be different at different numbers of cycles, e.g. the annealing ' temperature for each cycle may be reduced by 1 ℃ on the basis of the previous cycle, and the annealing ' temperature for the first cycle may be 65 ℃. The denaturation' conditions may be 94 ℃ for 30 s. The extension' conditions may be 72 ℃ for 2 min. The reaction conditions for PCR amplification using A1 are specifically shown in Table 2 in the examples.

In order to solve the technical problems, the invention also provides a method for identifying the lodging resistance character of the corn.

The method for identifying the lodging resistance character of the corn provided by the invention comprises the following steps: the method for identifying the maize genotype is used for identifying the genotype of the maize to be detected, and the maize to be detected with the BB genotype is lower than or is lower than the candidate of the lodging resistance of the maize to be detected with the BB genotype.

The method for identifying the lodging-resistant character of the corn comprises the following G1), G2), G3) and/or G4):

G1) identifying the plant height of corn, wherein the identification of the plant height of corn comprises the following steps: identifying the genotype of the corn to be detected by using the method for identifying the corn genotype, wherein the plant height of the corn to be detected of the BB genotype is lower than or is lower than the candidate corn to be detected of the BB genotype;

G2) identifying the ear height of the corn, wherein the identification of the ear height of the corn comprises the following steps: identifying the genotype of the corn to be detected by using the method for identifying the corn genotype, wherein the ear position of the corn to be detected of the BB genotype is lower than or is lower than the candidate corn to be detected of the BB genotype;

G3) identifying the emasculation plant height of the corn, wherein the identification of the emasculation plant height of the corn comprises the following steps: identifying the genotype of the corn to be detected by using the method for identifying the corn genotype, wherein the emasculated plant height of the corn to be detected of the BB genotype is lower than or is lower than the candidate corn to be detected of the BB genotype;

G4) identifying the relative ear height of the female ear of the corn, wherein the identification of the relative ear height of the female ear of the corn comprises the following steps: and identifying the genotype of the corn to be detected by using the method for identifying the corn genotype, wherein the relative ear height of the female ear of the corn to be detected of the BB genotype is lower than that of the corn to be detected of the BB genotype or the candidate corn to be detected of the BB genotype is lower than that of the BB genotype.

In order to solve the technical problems, the invention also provides a primer pair for identifying the lodging resistance character of the corn.

The primer pair for identifying the lodging resistance character of the corn provided by the invention is A1.

In order to solve the technical problems, the invention also provides the lodging-resistant molecular marker.

In order to solve the technical problems, the invention also provides the following 1), 2), 3), 4) or 5):

1) the A1 is applied to any one of the following H1-H11:

h1, preparing and identifying a corn lodging-resistant character product;

h2, preparing and identifying a corn plant height product;

h3, preparing and identifying a high product of the ear position of the corn;

h4, preparing and identifying a corn emasculation plant height product;

h5, preparing and identifying a product with high relative ear position of the corn ear;

h6, identifying the lodging resistance character of the corn;

h7, identifying the height of the corn;

h8, identifying the ear height of the corn;

h9, identifying the height of the emasculated corn;

h10, identifying the relative ear height of the corn ear;

h11, breeding corn;

2) detecting the application of the lodging-resistant molecular marker substance in any one of H1-H11;

3) use of the lodging resistant molecular marker in any of the above H1-H11;

4) the use of the method for identifying maize genotype in any one of H6-H11 described above;

5) the method for identifying the lodging-resistant character of the corn is applied to corn breeding.

In order to solve the technical problems, the invention also provides a corn breeding method.

According to the corn breeding method provided by the invention, the corn genotype is identified according to the method for identifying the corn genotype, and corn with Bb or Bb genotype is selected as a parent for breeding.

In the invention, the corn breeding can be the cultivation of lodging-resistant corn. The corn breeding can be specifically used for breeding corn with reduced plant height, breeding corn with reduced ear position of female ears, breeding emasculated plant height and reduced corn and/or breeding corn with reduced ear position relative to female ears.

The maize or the maize to be tested may be B1), B2), or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

The DNA fragments of 332bp and 100bp can be detected by electrophoresis and/or sequencing. When electrophoresis is used for detection, the 332bp DNA fragment can be embodied as a band between 250bp and 500bp, and the 100bp DNA fragment can be embodied as a band at 100 bp.

Experiments prove that the lodging resistance character of the corn can be identified by using the lodging resistance molecular marker of the invention: compared with the BB genotype at the 6377-6617 th site (i.e., both contain Br2 gene) of which both chromosomes contain the sequence 1, the plant height, the ear position, the emasculated strain height and the relative ear position height of the maize of which both chromosomes do not contain the 6377-6617 th site of the sequence 1 (i.e., both chromosomes contain the subbr 2 gene) are all significantly reduced (P < 0.01); the fundamental differences of the maize of the BB genotype and the BB genotype, such as the length of the male ear, the number of leaves on the female ear, the length of leaves on the female ear, the included angle of leaves and the diameter of a stem, are not obvious, and the maize of the BB genotype and the BB genotype has no obvious difference in the number of bracts, the length of the female ear, the number of rows of ears, the number of grains in rows and the diameter of a female ear axis. The main internal causes influencing the lodging of corn plants are: plant height, ear height and stalk diameter. And the bb genotype corn which lacks the DNA fragment shown in 6377-6617 th site of the sequence 1 can reduce the plant height and the ear height while keeping the diameter of the stem unchanged and has no obvious negative effect on the ear character which affects the yield, so that the corn has the effects of lodging resistance and stable yield like the green revolution genes in rice and wheat.

Drawings

FIG. 1 shows the results of differential analysis of plant height, ear length, relative ear height, tassel height, ear number, ear length, leaf angle and stem diameter of maize of BB genotype and BB genotype of different lines. ＊＊ shows that the differential reaches a significant level (P <0.01), and ＊ shows that the differential reaches a significant level (P < 0.05).

Fig. 2 shows the identification results of two parental maize inbred lines 52220 and maize inbred line Mo 18W. Where M is a molecular weight standard,

lanes

1 and 2 are both maize inbred Mo18W, and lanes 3 and 4 are both maize inbred 52220.

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention.

The experimental procedures in the following examples are conventional unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The maize inbred line Mo18W and maize inbred line 52220 in the following examples are from the american Germplasm resources bank (u.s.national Plant Germplasm System, website:https://npgsweb.ars-grin.gov/ gringlobal/search.aspx) The number of the maize inbred line Mo18W is PI 550441, and the number of the maize inbred line 52220 is Ames 2336. The plant height of the maize inbred line 52220 in Hainan third-generation to mature period is 70cm, and the plant height of the maize inbred line Mo18W in Hainan third-generation to mature period is 180 cm.

Example 1 lodging resistance molecular markers can be used to identify maize lodging resistance traits

The invention provides an anti-lodging molecular marker which is named as ZaRL, wherein the ZaRL is a DNA molecule obtained by taking the genomic DNA of corn as a template and adopting a primer pair A1 for amplification; a1 is composed of single-stranded DNAs designated P1 and P2, P1 is the single-stranded DNA shown at positions 6336-6355 of SEQ ID NO. 1, and P2 is the single-stranded DNA reverse-complementary to the single-stranded DNA shown at positions 6648-6667 of SEQ ID NO. 1.

The primer pair A1 is used for carrying out PCR amplification on genomic DNA of different maize strains, and the PCR products have two types, one type contains a1, the other type does not contain a1, and a1 is a DNA fragment shown in 6377-6617 th position of a sequence 1. Maize is classified into different genotypes according to the PCR products: the PCR product is a corn genotype containing a1 and is a BB genotype; the genotype of a corn containing a1 and a corn not containing a1 as a PCR product is Bb genotype; the genotype of a corn without a1 as a PCR product is bb genotype.

The corn inbred line 52220 and the corn inbred line Mo18W are hybridized to form an F2 population, the F2 population is inbred to obtain an F3 population, and the F3 population is inbred to obtain an F4 population.

The primer pair A1 is used for identifying the genotypes of the F4 population, and NIL-like materials of 4 Bb genotypes are selected, and the specific identification method is as follows:

extracting corn genome DNA, and carrying out PCR amplification on the corn genome DNA by using a primer pair A1, wherein the reaction system is shown in Table 1.

TABLE 1 PCR reaction System

Components	Volume (μ l)
		2 XGC Buffer I (product of TaKaRa)	5
dNTP mix (product of TaKaRa)	2
		P1(10pmol/μL)	0.2
P2(10pmol/μL)	0.2
		ddH₂O	2
TaKaRa LA Taq (5U/. mu.l) (product of TaKaRa)	0.1
		DNA	0.5
Total volume	10

The reaction conditions are shown in Table 2.

TABLE 2 PCR reaction conditions

The obtained PCR products were subjected to electrophoresis, and the electrophoresis results of two parents (maize inbred line 52220 and maize inbred line Mo18W) are shown in table 2. Selecting PCR products as two bands with the size difference of about 232bp for recovery and sequencing, and finally selecting 4 NIL-like materials (the serial numbers of the 4 NIL-like materials are respectively 105, 114, 162 and 17), wherein the genotypes of the 4 NIL-like materials are Bb genotypes, the PCR products contain two bands, the sequence of the DNA fragment corresponding to one band of the two bands is the 6336-th and 6667-th positions of the sequence 1, and the sequence of the DNA fragment corresponding to the other band is the 6328-th and 6427-th positions of the sequence 2.

Seeds of these 4 NIL-like line materials were sown in the field (at beijing, 5 months old) one plot per material. The genotypes of the progeny of these 4 NIL-like lines of material were identified by primer pair A1 as described above, each material having three genotypes, namely the BB genotype, the Bb genotype and the BB genotype, wherein the PCR product of the BB genotype contained the DNA fragment represented at position 6336-.

When the corn is mature, the plant heights of the BB genotype, the Bb genotype and the BB genotype, the ear height of the female ear, the height of the tassel removed, the relative ear height (relative ear height is equal to ear height/plant height), the leaf number on the ear of the female ear, the length of the male ear, the leaf length on the ear of the female ear, the leaf included angle (the leaf included angle of the first leaf on the upper ear), the diameter of the stem (the diameter of the third internode on the upper part of the main stem stalk and the middle short diameter (excluding the leaf sheath)) and the ear stalk length, the bud number, the ear length, the line number, the row number and the ear axis diameter of the female ear are counted in each cell.

Plant height, ear position height, ear length, relative ear position height, emasculated ear plant height, number of leaves on the ear, leaf length on the ear, leaf angle and stem diameter of maize of BB genotype and BB genotype of different lines are shown in Table 1, and differences among different genotypes of the same line are analyzed (FIG. 1).

TABLE 3 phenotype of BB genotype and BB genotype of different lines

As a result, in the 4 materials with the numbers of 105, 114, 162 and 17, the plant height, the height of the female ear, the height of the emasculated ear and the height of the relative ear of the corn were significantly reduced (P <0.01) compared with the BB genotype, the BB genotype, and the plant height, the height of the female ear, the height of the emasculated ear and the height of the relative ear of the corn were not significantly different from the Bb genotype. The lodging-resistant molecular marker is related to the lodging resistance of corn. Apart from the tassel length of 114, the leaf length on the ear of 17 and 162, and the diameter of the stem of 105, the difference in tassel length, leaf number on the ear, leaf length on the ear, leaf angle and stem diameter between the BB genotype and BB genotype maize was not significant (table 3), nor was the difference in tassel length, leaf number on the ear, leaf length on the ear, leaf angle and stem diameter between the BB genotype and BB genotype. Further, the comparison of the ear properties of the female ears revealed that, in addition to the ear stalk length, there was no significant difference in the number of bracts, the length of the female ears, the number of rows, the number of grains, and the diameter of the female ear axis between the BB genotype and BB genotype of corn (table 3), and that there was no significant difference in the number of bracts, the length of the female ears, the number of rows, the number of grains, and the diameter of the female ear axis between the BB genotype and BB genotype.

The main internal causes influencing the lodging of corn plants are: plant height, ear height and stem diameter. And the family plant lacking the DNA fragment shown in 6377-6617 th site of the sequence 1 can reduce the plant height and the ear height while keeping the diameter of the stem unchanged and has no obvious negative effect on the ear character influencing the yield, so that the family plant has the effects of lodging resistance and stable yield like the green revolution genes in rice and wheat. The inventors named the allele with the complete gene structure from which the DNA fragment shown in 6377-6617 of SEQ ID NO. 1 had been deleted as the subbr 2 gene.

To confirm the influence of the subbr 2 gene on plant height and yield-related traits, the inventors hybridized the extremely short-stalk maize inbred line 52220 with the backbone maize inbred line Mo18W to form an F2 population. 215 individuals are selected from the population for GBS sequencing, and a high-density linkage map with the average density of 1SNP/100kb is constructed. Through detection, the plant height trait is normally distributed in an F2 population, and a main effect QTL exists on a chromosome 1 from 80cM to 110cM, so that 26% of phenotypic variation can be explained. To further refine the localization, the inventors designed SSR markers within this segment to initially localize this QTL between markers P1 and P7, screened for crossovers, and mapped the progeny F3 of 2319 strain derived from 12F 3 crossover individuals: and 4, continuously encrypting the mark for screening. Genotype and phenotype correlations were examined for F4 population pedigrees by linear regression models. The target segment is finally narrowed down to a range of 200.8Mb to 208.3Mb of chromosome one. The Br2 is finally determined as a candidate gene by performing functional analysis on 182 annotated genes in the segment and comparing genes on sorghum and rice chromosome segments which are homologous with the segments. By sequencing comparison, the Br2 gene on Mo18W consists of 5 exons and 4 introns, and the total length is 6657 bp. Wherein the coding sequence (CDS) of 4140bp finally codes to generate a protein containing 1379 amino acids. The protein contains two ABC transporter 1-type transmembrane structures (118aa to 441aa and 785aa to 1,105aa) and two P-ring nucleoside hydrolase structure thresholds (463aa to 699aa and 1,121aa to 1,357aa) through a viewing domain of an Interpro database. In the dwarf parent 52220, the br2 gene (subbr 2 gene) has 241bp deleted in 6377bp to 6617bp position in the fifth exon to result in the translational inactivation of the second P-ring nucleotide hydrolase domain. The subbr 2 gene can be used for cultivating lodging-resistant corn. The Br2 gene and the subr2 gene are alleles.

<110> university of agriculture in China

<120> lodging-resistant molecular marker and application of polymorphism thereof in identification of lodging-resistant character of corn

<160>2

<170>PatentIn version 3.5

<210>1

<211>7017

<212>DNA

<213> corn

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gaagagcacg gtggtgtcgc tcatcgagag gttctacgac cccagcgcag gtatactacc 1800

tagtactgtt actactttta gcgcattaat ctgaggatgt ccagttcgct tgcttgccaa 1860

tcgccattgc catcgcaaca acaatacatt gccaactgcc attgctgggt agactagtac 1920

agtagcagtt agaagaagcc tccactgtac attgcattgc caaacaaaag tgaattgtgc 1980

agtaactctg taccaccaca ttgacatgga aatgaagtga atgcttggag catgcagagc 2040

tggccggcct catgggctgc tgctacctgc tagctagcca accagaacca gccatcctct 2100

ttcttgcttt tctttttact ttctttggtc gtggctgttt gtggtcatac atacattcac 2160

gcagagcaga agagctagct aagctaggtg ggtgtgcctg caacgcggga caaagaaaac 2220

tatttgttgc ctggcaagat gctactgttg cctagcacat gcctgccatt gaccgactgc 2280

tcagtgagaa gtggttcagt tgtgctgttg acagtataga tagatatata tagtagccct 2340

gtagattttt ttttcagaca aaaaaagaag aagaacgaga tgaagtctgc aattcggttt 2400

tggcagggca aatcctgctg gacgggcacg acctcaggtc gctggagctg cggtggctgc 2460

ggcggcagat cgggctggtg agccaggagc cggcgctgtt cgcgacgagc atcagggaga 2520

acctgctgct ggggcgggac agccagagcg cgacgctggc ggagatggag gaggcggcca 2580

gggtggccaa cgcccactcc ttcatcatca aactccccga cggctacgac acgcaggtcc 2640

gtcccgtata gctagctcac tagctgcact gccacttctc tcgcttgctc cccaccgttg 2700

ctgcctgttg ctctccatcc acttgtcggt gtctggacca cacgtgcctg cttgcctagc 2760

tgctccacat ctgctttccc tgtccaacct tatgcaactc actctatata ctatatcaaa 2820

tacatttcta gagtttaaag cttatcttag aataaatgca tctttagcta cgagacaacc 2880

taacttcagt tgttgttgtt ttttttactt tctctcttct caaaaataca ttgattacgt 2940

ctttacagcg atctttttta ttccaaacct aaaaatgcat gcactcactc taaaagcgca 3000

aagggagcat cttttttttc ccccatcatc tgcacgcagc cttttctttt cctcatgtca 3060

cgagggactg aaggtgtgta tgcagcgtca agtcatccat ccgttccact ccactcactc 3120

atgcgtcgcg cactctgcgc tcgtgcctgc ccggggctaa agctttagta gctagcctca 3180

gatcagatac tgttcgtgtt tgttaggccg cggcagctgc acatgagctc atgacagccg 3240

gcagcaccac caccaacgcc atggaagagg ggtcggggtc catcacatag acatagatgc 3300

ctgttgtaga ctaggacggg agggcaattg ttaggcgcct gttgccatcg catttgctgc 3360

tgtgggttgc caacaagtaa catgccagga tgctttgcta tcacgcacag gacaggagag 3420

gtcctttttc tcgacacaag ctctacagcc tctactaaac tagcacttgc tgatgagtgc 3480

agaggatgaa tggacgatga acatctagag tgagagagaa aaaaatgtta ataataataa 3540

aaagtagtag caggattaag aatcaacctg gggtacgtag gaagaggtac aatccctagg 3600

aatctagagt atgagaagta tgggaggagt tgtgagtgaa acggaacaaa ttccgagttg 3660

gtattttgtc gggaatgtca agttgatttt tgatcctagt gcaagcaaga attatcaatc 3720

actcagactc agcctgtctg tgtctgtcca ccccagctct tgctactcta cttactactg 3780

tgctactagt ggtagggtag gtatcttcca taaactgtta ttataaactg tcatctgaga 3840

aagagagcca gtcaaaccca tgctgctgct tatttttaat cactgtcaaa tggcaggcag 3900

gcaggcagtc tggttagtta ataacatctg ggaagggttt aatcaaacca aatcaaatca 3960

tacgaaatct agaggccaca tgggatgggg ccatatgtac tgtactagca taagtagcgg 4020

ctagatttta ttagaacacg gactcacact cccataacta taactgactt tgatcatgat 4080

tccttgccaa gcaatgctcg catgcccatg catgcatcat ccctggtcaa actcaaacac 4140

tctccaccgt cagggaataa gacttattat tttattaaca attccatttt tatttattaa 4200

ttacggctgg gcgaggagta ctagtttatt tgatgagaga catggcagtc caagtcaaac 4260

tcgtttgtct gaccatggcg gtgatggccg gttgcaggtt ggggagcgcg gcctgcagct 4320

ctccggtggg cagaagcagc gcatcgccat cgcccgcgcc atgctcaaga accccgccat 4380

cctgctgctg gacgaggcca ccagcgcgct ggactccgag tctgagaagc tcgtgcagga 4440

ggcgctggac cgcttcatga tcgggcgcac caccctggtg atcgcgcaca ggctgtccac 4500

catccgaaag gccgacgtgg tggccgtgct gcagggcggc gccgtctccg agatgggcgc 4560

gcacgacgag ctgatggcca agggcgagaa cggcacctac gccaagctca tccgcatgca 4620

ggagcaggcg cacgaggcgg cgctcgtcaa cgcccgccgc agcagcgcca ggccctccag 4680

cgcccgcaac tccgtcagct cgcccatcat gacgcgcaac tcctcctacg gccgctcccc 4740

ctactcccgc cgcctctccg acttctccac ctccgacttc accctctcca tccacgaccc 4800

gcaccaccac caccggacca tggcggacaa gcagctggcg ttccgcgccg gcgccagctc 4860

cttcctgcgc ctcgccagga tgaactcgcc cgagtgggcc tacgcgctcg ccggctccat 4920

cggctccatg gtctgcggct ccttcagcgc catcttcgcc tacatcctca gcgccgtgct 4980

cagcgtgtac tacgcgccgg acccgcggta catgaagcgc gagatcgcca aatactgcta 5040

cctgctcatc ggcatgtcct ccgcggcgct gctgttcaac acggtgcagc acgtgttctg 5100

ggacacggtg ggcgagaacc tgaccaagcg ggtgcgcgag aagatgttcg ccgccgtgct 5160

ccgcaacgag atcgcctggt tcgacgcgga cgagaacgcc agcgcgcgcg tggccgccag 5220

gctcgcgctg gacgcccaga acgtgcgctc cgccatcggg gaccgcatct ccgtcatcgt 5280

ccagaactcg gcgctgatgc tggtggcctg caccgcgggg ttcgtcctcc agtggcgcct 5340

cgcgctcgtg ctcctcgccg tgttcccgct cgtcgtgggc gccaccgtgc tgcagaagat 5400

gttcatgaag ggcttctcgg gggacctgga ggccgcgcac gccagggcca cgcagatcgc 5460

gggcgaggcc gtggccaacc tgcgcaccgt ggccgcgttc aacgcggagc gcaagatcac 5520

ggggctgttc gaggccaacc tgcgcggccc gctccggcgc tgcttctgga aggggcagat 5580

cgccggcagc ggctacggcg tggcgcagtt cctgctgtac gcgtcctacg cgctggggct 5640

gtggtacgcg gcgtggctgg tgaagcacgg cgtgtccgac ttctcgcgca ccatccgcgt 5700

gttcatggtg ctgatggtgt ccgcgaacgg cgccgccgag acgctgacgc tggcgccgga 5760

cttcatcaag ggcgggcgcg cgatgcggtc ggtgttcgag acgatcgacc gcaagacgga 5820

ggtggagccc gacgacgtgg acgcggcgcc ggtgccggag cggccgaggg gcgaggtgga 5880

gctgaagcac gtggacttct cgtacccgtc gcggccggac atccaggtgt tccgcgacct 5940

gagcctccgt gcgcgcgccg ggaagacgct ggcgctggtg gggccgagcg ggtgcggcaa 6000

gagctcggtg ctggcgctgg tgcagcggtt ctacgagccc acgtccgggc gcgtgctcct 6060

ggacggcaag gacgtgcgca agtacaacct gcgggcgctg cggcgcgtgg tggcggtggt 6120

gccgcaggag ccgttcctgt tcgcggcgag catccacgag aacatcgcgt acgggcgcga 6180

gggcgcgacg gaggcggagg tggtggaggc ggcggcgcag gcgaacgcgc accggttcat 6240

cgcggcgctg ccggaggggt accggacgca ggtgggcgag cgcggggtgc agctgtcggg 6300

ggggcagcgg cagcggatcg cgatcgcgcg cgcgctggtg aagcaggcgg ccatcgtgct 6360

gctggacgag gcgaccagcg cgctggacgc cgagtcggag cggtgcgtgc aggaggcgct 6420

ggagcgcgcg gggtccgggc gcaccaccat cgtggtggcg caccggctgg ccacggtgcg 6480

cggcgcgcac accatcgcgg tcatcgacga cggcaaggtg gcggagcagg ggtcgcactc 6540

gcacctgctc aagcaccatc ccgacgggtg ctacgcgcgg atgctgcagc tgcagcggct 6600

gacgggcgcg gcggccgggc ccgggccgtc gacctcgtgc aacggggccg cgtaggacgg 6660

aatggatgga tggatgggtt tggttcctcg agagattgat gggtgaggaa gctgaagctc 6720

cggatcaaat ggtggtactc catgatcgca acaatgaggg gaaaaaaaga aaggagaaaa 6780

tacggtggtt catatgattg tacaatttga cgatctgttt gagtcggggt tttaggatga 6840

tgtaaacctt cactcgcctt ttttttactc ttgtttctca tccgcatcag tatcatctat 6900

ctacatacag tgtcagagat gggaactgat ccgcatcatc atctacctcc caaggcaccc 6960

cagattgtat taatgtactt agttagcctg ttttatatat acttataagt accaaat 7017

<210>2

<211>6777

<212>DNA

<213> corn

<400>2

atgtctagca gcgacccgga ggagatcagg gcgcgcgtcg tcgttctcgg ttcgccccat 60

gccgacggcg gcgacgagtg ggcccggccc gagctcgagg ccttccatct gccgtctccc 120

gcccaccagc ctcctggctt cctagccggg caaccggaag cagcagagca acccacgctc 180

cctgctcatg ctggccgcag cagcagcagc aacacgccta ctacatctgc cggtggcggc 240

gctgctcctc ctcttccttc ttcgcctccc cctccgtcgg cttctctgga gaccgagcag 300

ccgcccaatg ccaggccagc ctccgccggc gccaatgaca gcaagaagcc caccccgccc 360

gccgccctgc gcgacctctt ccgcttcgcc gacggcctcg actgcgcgct catgctcatc 420

ggcaccctcg gtgcgctcgt ccacgggtgc tcgctccccg tcttcctccg cttcttcgcc 480

gacctcgtcg actccttcgg ctcccacgcc gacgacccgg acaccatggt ccgcctcgtc 540

gtcaagtatg ccttctactt cctcgtcgtc ggagcggcaa tctgggcatc ctcgtgggca 600

ggtacgctat ccctcctcct cttgccgccc cagcttgtgt gcgtcgcgaa ttggcggtca 660

atttggattg gatgacaaat cacgtcggtc agtcaatagc catggctaca aacgagatgt 720

tcaaatcgtt cgccccgctc gcagagatct cttgctggat gtggaccggc gagcggcagt 780

cgacgcggat gcggatccgg tacctggacg cggcgctgcg gcaggacgtg tccttcttcg 840

acaccgacgt gcgggcctcg gacgtgatct acgccatcaa cgcggacgca gtggtggtgc 900

aggacgccat cagcgagaag ctgggcaacc tcatccacta catggccacc ttcgtggccg 960

gcttcgtcgt ggggttcacg gccgcgtggc agctggcgct ggtcacgctg gccgtggtgc 1020

cgctcatcgc cgtcatcggc gggctgagcg ccgccgcgct cgccaagctc tcgtcccgca 1080

gccaggacgc gctctccggc gccagcggca tcgcggagca ggcgctcgcg cagatacgga 1140

tcgtgcaggc gttcgttggc gaggagcgcg agatgcgggc ctactcggca gcgctggccg 1200

tggcgcagag gatcggctac cgcagcggct tcgccaaggg gctcggcctc ggcggcacct 1260

acttcaccgt cttctgctgc tacgggctcc tgctctggta cggcggccac ctcgtgcgcg 1320

cccagcacac caacggcggg ctcgccatcg ccaccatgtt ctccgtcatg atcggcggac 1380

tgtaaggccc accacaccac tctctccttc tcctgctcct cggcccgccc gtcgtcattg 1440

ctgctgacgg tgtctgtgga tcgcgtgcag ggccctcggg cagtcggcgc cgagcatggc 1500

cgcgttcgcc aaggcgcgcg tggcggccgc caagatcttc cgcatcatcg accacaggcc 1560

gggcatctcc tcgcgcgacg gcgaggacgg cgcggagcca gagtcggtga cggggcgggt 1620

ggagatgcgg ggcgtggact tcgcgtaccc gtcgcggccg gacgtcccca tcctgcgcgg 1680

cttctcgctg agcgtgcccg ccgggaagac catcgcgctg gtgggcagct ccggctccgg 1740

gaagagcacg gtggtgtcgc tcatcgagag gttctacgac cccagcgcag gtatactacc 1800

tagtactgtt actactttta gcgcattaat ctgaggatgt ccagttcgct tgcttgccaa 1860

tcgccattgc catcgcaaca acaatacatt gccaactgcc attgctgggt agactagtac 1920

agtagcagtt agaagaagcc tccactgtac attgcattgc caaacaaaag tgaattgtgc 1980

agtaactctg taccaccaca ttgacatgga aatgaagtga atgcttggag catgcagagc 2040

tggccggcct catgggctgc tgctacctgc tagctagcca accagaacca gccatcctct 2100

ttcttgcttt tctttttact ttctttggtc gtggctgttt gtggtcatac atacattcac 2160

gcagagcaga agagctagct aagctaggtg ggtgtgcctg caacgcggga caaagaaaac 2220

tatttgttgc ctggcaagat gctactgttg cctagcacat gcctgccatt gaccgactgc 2280

tcagtgagaa gtggttcagt tgtgctgttg acagtataga tagatatata tagtagccct 2340

gtagattttt ttttcagaca aaaaaagaag aagaacgaga tgaagtctgc aattcggttt 2400

tggcagggca aatcctgctg gacgggcacg acctcaggtc gctggagctg cggtggctgc 2460

ggcggcagat cgggctggtg agccaggagc cggcgctgtt cgcgacgagc atcagggaga 2520

acctgctgct ggggcgggac agccagagcg cgacgctggc ggagatggag gaggcggcca 2580

gggtggccaa cgcccactcc ttcatcatca aactccccga cggctacgac acgcaggtcc 2640

gtcccgtata gctagctcac tagctgcact gccacttctc tcgcttgttc cccaccgtgc 2700

tgcctgttgc tctccatcca cttgtcggtg tctggaccac acgtgcctgc ttgcctagct 2760

gctccacatc tgctttccct gtccaacctt atgcaactca ctctatacta tatcaaatac 2820

atttctagag tttaaagctt atcttagaat aaatgcatct ttagctacga gacaacctaa 2880

cttcagttgt tgttgttttt tttactttct ctcttctcaa aaatacattg attacgtctt 2940

tacagcgatc ttttttattc caaacctaaa aatgcatgca ctcactctaa aagcgcaaag 3000

ggagcatctt ttttttcccc catcatctgc acgcagcctt ttcttttcct catgtcacga 3060

gggactgaag gtgtgtatgc agcgtcaagt catccatccg ttccactcac tcatgcgtcg 3120

cgcactctgc gctcgtgcct gcccggggct aaagctttag tagctagcct cagatcagat 3180

actgttcgtg tttgttaggc cgcggcagct gcacatgagc tcatgacagc cggcagcacc 3240

accaccaacg ccatggaaga ggggtcgggg tccatcacat agacatagat gcctgttgta 3300

gactaggacg ggagggcaat tgttaggcgc ctgttgccat cgcatttgct gctgtgggtt 3360

gccaacaagt aacatgccag gatgctttgc tatcacgcac aggacaggag aggtcctttt 3420

tctcgacaca agctctacag cctctactaa actagcactt gctgatgagt gcagaggatg 3480

aatggacgat gaacatctag agtgagagag aaaaaaatgt taataataat aaaaagtagt 3540

agcaggatta agaatcaacc tggggtacgt aggaagaggt acaatcccta ggaatctaga 3600

gtatgagaag tatgggagga gttgtgagtg aaacggaaca aattccgagt tggtattttg 3660

tcgggaatgt caagttgatt tttgatccta gtgcaagcaa gaattatcaa tcactcagac 3720

tcagcctgtc tgtgtctgtc caccccagct cttgctactc tacttactac tgtgctacta 3780

gtggtagggt aggtatcttc cataaactgt tattataaac tgtcatctga gaaagagagc 3840

cagtcaaacc catgctgctg cttattttta atcactgtca aatggcaggc aggcaggcag 3900

tctggttagt taataacatc tgggaagggt ttaatcaaac caaatcaaat catacgaaat 3960

ctagaggcca catgggatgg ggccatatgt actgtactag cataagtagc ggctagattt 4020

tattagaaca cggactcaca ctcccataac tataactgac tttgatcatg attccttgcc 4080

aagcaatgct cgcatgccca tgcatgcatc atccctggtc aaactcaaac actctccacc 4140

gtcagggaat aagacttatt attttattaa caattccatt tttatttatt aattacggct 4200

ggacgaggag tactagttta tttgatgaga gacatggcag tccaagtcaa actcgtttgt 4260

ctgaccatgg cggtgatggc cggttgcagg ttggggagcg cggcctgcag ctctccggtg 4320

ggcagaagca gcgcatcgcc atcgcccgcg ccatgctcaa gaaccccgcc atcctgctgc 4380

tggacgaggc caccagcgcg ctggactccg agtctgagaa gctcgtgcag gaggcgctgg 4440

accgcttcat gatcgggcgc accaccctgg tgatcgcgca caggctgtcc accatccgca 4500

aggccgacgt ggtggccgtg ctgcagggcg gcgccgtctc cgagatgggc gcgcacgacg 4560

agctgatggc caagggcgag aacggcacct acgccaagct catccgcatg caggagcagg 4620

cgcacgaggc ggcgctcgtc aacgcccgcc gcagcagcgc caggccctcc agcgcccgca 4680

actccgtcag ctcgcccatc atgacgcgca actcctccta cggccgctcc ccctactccc 4740

gccgcctctc cgacttctcc acctccgact tcaccctctc catccacgac ccgcaccacc 4800

accaccggac catggcggac aagcagctgg cgttccgcgc cggcgccagc tccttcctgc 4860

gcctcgctag gatgaactcg cccgagtggg cctacgcgct cgccggctcc atcggctcca 4920

tggtctgcgg ctccttcagc gccatcttcg cctacatcct cagcgccgtg ctcagcgtgt 4980

actacgcgcc ggacccgcgg tacatgaagc gcgagatcgc caaatactgc tacctgctca 5040

tcggcatgtc ctccgctgcg ctgctgttca acacggtgca gcacgtgttc tgggacacgg 5100

tgggcgagaa cctgaccaag cgggtgcgcg agaagatgtt cgccgccgtg ctccgcaacg 5160

agatcgcctg gttcgacgcg gacgagaacg ccagcgcgcg cgtggccgcc aggctcgcgc 5220

tggacgccca gaacgtgcgc tccgccatcg gggaccgcat ctccgtcatc gtccagaact 5280

cggcgctgat gctggtggcc tgcaccgcgg ggttcgtcct ccagtggcgc ctcgcgctcg 5340

tgctcctcgc cgtgttcccg ctcgtcgtgg gcgccaccgt gctgcagaag atgttcatga 5400

agggcttctc gggggacctg gaggccgcgc acgccagggc gacgcagatc gcgggcgagg 5460

ccgtggccaa cctgcgcacc gtggccgcgt tcaacgcgga gcgcaagatc acggggctgt 5520

tcgaggccaa cctgcgcggc ccgctccggc gctgcttctg gaaggggcag atcgccggca 5580

gcggctacgg cgtggcgcag ttcctgctgt acgcgtccta cgcgctgggg ctgtggtacg 5640

cggcgtggct ggtgaagcac ggcgtgtccg acttctcgcg caccatccgc gtgttcatgg 5700

tgctgatggt gtccgcgaac ggcgccgccg agacgctgac gctggcgccg gacttcatca 5760

agggcgggcg cgcgatgcgg tcggtgttcg agacgatcga ccgcaagacg gaggtggagc 5820

ccgacgacgt ggacgcggcg ccggtgccgg agcggccgag gggcgaggtg gagctgaagc 5880

acgtggactt ctcgtacccg tcgcggccgg acatccaggt gttccgcgac ctgagcctcc 5940

gtgcgcgcgc cgggaagacg ctggcgctgg tggggccgag cgggtgcggc aagagctcgg 6000

tgctggctct ggtgcagcgg ttctacgagc ccacgtccgg gcgcgtgctc ctggacggca 6060

aggacgtgcg caagtacaac ctgcgggcgc tgcggcgcgt ggtggcggtg gtgccgcagg 6120

agccgttcct gttcgcggcg agcatccacg agaacatcgc gtacgggcgc gagggcgcga 6180

cggaggcgga ggtggtggag gcggcggcgc aggcgaacgc gcaccggttc atcgcggcgc 6240

tgccggaggg gtaccggacg caggtgggcg agcgcggggt gcagctgtcg ggagggcagc 6300

ggcagcggat cgcgatcgcg cgcgcgctgg tgaagcaggc ggccatcgtg ctgctggacg 6360

aggcgacctc gtgcaacggc ccgggccgtc gtcctcgtgc aacggggccg cgtaggacgg 6420

aatggatgga tggatgggtt tggttcctcg agagattgat gggtgaggaa gctgaagctc 6480

cggatcaaat ggtggtactc catgatcgca acaatgaggg gaaaaaaaga aaggagaaaa 6540

tacggtggtt catatgattg tacaatttga cgatctgttt gagtcggggt tttaggatga 6600

tgtaaacctt cactcgcctt ttttttactc ttgtttctca tccgcatcag tatcatctat 6660

ctacatacag tgtcagagat gggaactgat ccgcatcatc atctacctcc caaggcaccc 6720

cagattgtat taatgtactt agttagcctg ttttatatat acttataagt accaaat 6777

Claims

1. The application of the lodging-resistant molecular marker in identifying the lodging-resistant character of the corn;

the lodging-resistant molecule is marked as a DNA molecule shown in the 6336-6667 th position of the sequence 1 or the 6328-6427 th position of the sequence 2.

2. Use according to claim 1, characterized in that: the lodging resistance of the corn is embodied in plant height, ear position of the female ear, emasculation plant height and/or relative ear position of the female ear.

3. Use according to claim 1 or 2, characterized in that: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

4. A method of identifying maize genotypes, the genotypes being a BB genotype, and a BB genotype, the method comprising: detecting a DNA fragment corresponding to the 6377-6632-th site of the sequence 1 in the sequence table in the to-be-detected corn genome DNA, wherein the to-be-detected corn is a BB genotype if two chromosomes in the to-be-detected corn genome are the chromosomes of h 1); if the two chromosomes in the genome of the maize to be detected are both the chromosomes of h2), the maize to be detected is the bb genotype; if one of the two chromosomes in the maize genome to be detected is the chromosome of h1) below and the other chromosome is the chromosome of h2) below, the maize to be detected is the Bb genotype;

h2) the 6377-6632 bit corresponding to the sequence 1 in the sequence table is the 6369-6392 bit of the sequence 2.

5. The method of claim 4, wherein: the method includes L1) and L2) as follows:

l1) taking corn genome DNA to be detected as a template, and carrying out PCR amplification on A1 by adopting a primer pair to obtain a PCR product; the A1 consists of single-stranded DNA with the names of P1 and P2, the P1 is the single-stranded DNA shown in the 6336-6355 position of the sequence 1, and the P2 is the single-stranded DNA which is reversely complementary with the single-stranded DNA shown in the 6648-6667 position of the sequence 1;

l2) the following L21) or L22):

6. The method according to claim 4 or 5, characterized in that: the corn to be tested is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

7. The primer pair for identifying the lodging-resistant character of the corn is a primer pair consisting of single-stranded DNAs (deoxyribonucleic acids) named as P1 and P2, wherein the P1 is the single-stranded DNA shown at the 6336-6355 position of the sequence 1, and the P2 is the single-stranded DNA reverse-complementary to the single-stranded DNA shown at the 6648-6667 position of the sequence 1.

8. The primer pair according to claim 7, wherein: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

9. The lodging-resistant molecular marker is a DNA molecule shown in the 6336-6667 th position of the sequence 1 or the 6328-6427 th position of the sequence 2.

Use of a1 in any one of the following H1-H11:

h1, preparing and identifying a corn lodging-resistant character product;

h2, preparing and identifying a corn plant height product;

h3, preparing and identifying a high product of the ear position of the corn;

h4, preparing and identifying a corn emasculation plant height product;

h6, identifying the lodging resistance character of the corn;

h7, identifying the height of the corn;

h8, identifying the ear height of the corn;

h9, identifying the height of the emasculated corn;

h10, identifying the relative ear height of the corn ear;

h11, breeding corn;

the A1 is a primer pair consisting of single-stranded DNA named P1 and P2, the P1 is the single-stranded DNA shown in the 6336-6355 position of the sequence 1, and the P2 is the single-stranded DNA reverse-complementary to the single-stranded DNA shown in the 6648-6667 position of the sequence 1.

11. Use according to claim 10, characterized in that: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

12. The application of the substance for detecting the lodging-resistant molecular marker in any one of the following H1-H11:

h1, preparing and identifying a corn lodging-resistant character product;

h2, preparing and identifying a corn plant height product;

h3, preparing and identifying a high product of the ear position of the corn;

h4, preparing and identifying a corn emasculation plant height product;

h6, identifying the lodging resistance character of the corn;

h7, identifying the height of the corn;

h8, identifying the ear height of the corn;

h9, identifying the height of the emasculated corn;

h10, identifying the relative ear height of the corn ear;

h11, breeding corn;

13. Use according to claim 12, characterized in that: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

14. The use of a lodging resistant molecular marker in any one of the following H1-H11:

h1, preparing and identifying a corn lodging-resistant character product;

h2, preparing and identifying a corn plant height product;

h3, preparing and identifying a high product of the ear position of the corn;

h4, preparing and identifying a corn emasculation plant height product;

h6, identifying the lodging resistance character of the corn;

h7, identifying the height of the corn;

h8, identifying the ear height of the corn;

h9, identifying the height of the emasculated corn;

h10, identifying the relative ear height of the corn ear;

h11, breeding corn;

15. Use according to claim 14, characterized in that: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

16. Use of the method of identifying a maize genotype of claim 4 or 5 in any one of H6-H11;

h6, identifying the lodging resistance character of the corn;

h7, identifying the height of the corn;

h8, identifying the ear height of the corn;

h9, identifying the height of the emasculated corn;

h10, identifying the relative ear height of the corn ear;

h11 and breeding corn.

17. Use according to claim 16, characterized in that: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.

18. A method for breeding corn, wherein the corn is genotyped according to the method of claim 4 or 5, and the corn with Bb or Bb genotype is selected as parent for breeding.

19. The method of claim 18, further comprising: the corn is B1), B2) or B3):

B1) maize inbred line 52220 or progeny thereof as a parent;

B2) maize inbred line Mo18W or its progeny as a parent;

B3) progeny of the maize inbred line 52220 and maize inbred line Mo 18W.