CN106544245A - A kind of cordyceps wine preparation method attractive in appearance - Google Patents
A kind of cordyceps wine preparation method attractive in appearance Download PDFInfo
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- CN106544245A CN106544245A CN201510604456.3A CN201510604456A CN106544245A CN 106544245 A CN106544245 A CN 106544245A CN 201510604456 A CN201510604456 A CN 201510604456A CN 106544245 A CN106544245 A CN 106544245A
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Abstract
The present invention relates to a kind of preparation method of cordyceps wine attractive in appearance, it is characterised in that the method comprises the steps：Step 1, in the Cordyceps militaris (L.) Link. solid culture stage, fraction solids culture medium is separated according to the shape for being placed in container from overall broth together with coremium therein is grown on；Detached culture medium and growth coremium therein are placed in container by step 2；Solid medium is fixed on container bottom with supporter by step 3；Step 4, notes wine in container；With step 5, more than 30 days are fixed, take out supporter.Cordyceps wine prepared by the present invention does not only have higher nutritive value, and unique aesthetic.
Description
Technical field
The present invention relates to a kind of cordyceps wine preparation method attractive in appearance, and in particular to a kind of using specific
The method of grain culture medium production " the long Cordyceps militaris (L.) Link. in wine jar " wine, belongs to medicine food field.
Background technology
Cordyceps militaris (L.) Link. (Cordyceps) is Ascomycotina, Ergota Zoopagales, Cordycepses section, the pattern of Cordyceps
Kind, scientific name is [Cordyceps militaris (Vuill.) Fr.].Modern science not only has in proving Cordyceps militaris (L.) Link.
There is special nutritive value, and have obvious medical value.Wherein especially with cordycepic acid, cordycepin,
Based on adenosine, Cordyceps polysaccharide.With functions such as tonifying the lung the moon, kidney-replenishings, the Main Function of Cordyceps militaris (L.) Link. is
Control kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, eak after being ill.The traditional Chinese medical science thinks which plays strengthening the body resistance effect,
There is significant curative effect to senile chronic bronchitis, pulmonary heart disease, liver detoxification ability can be improved,
Liver protection effect is played, body antiviral and capability of resistance to radiation is improved.The traditional Chinese medical science thinks that Cordycepses enter lung kidney two warp,
Can tonifying the lung it is cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, eak after being ill,
Chronic cough is weak, phthisical cough expectorant blood, and spontaneous sweating etc., is that unique one kind can be while balancing, adjusting negative and positive
Chinese medicine.
It is the usual alimentation method for adopting that Cordyceps militaris (L.) Link. is steep in wine, and its brew process is：By Cordyceps militaris (L.) Link.
(can also be brewed together with other drugs, such as Radix Ginseng, Fructus Lycii etc.) be placed in the vessel and pours into white
Wine, seal, to place appropriate natural law i.e. drinkable.Using this brewing method, Cordyceps militaris (L.) Link. is using existing
Coremium into culture is placed in a reservoir, it is at random and have swim in wine list layer, its effective ingredient
It is not completely immersed in wine, and does not show good situation that Cordyceps militaris (L.) Link. is grown in wine, does not project
The beautiful impression of Cordycepses vision.The characteristic of the ancient spirits culture of China is not presented.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cordyceps wine attractive in appearance, which does not only have higher
Nutritive value, and unique aesthetic.
The purpose of the present invention is achieved by the following technical solution：
A kind of preparation method of cordyceps wine attractive in appearance, the method comprise the steps：
Step 1, it is in the Cordyceps militaris (L.) Link. solid culture stage, fraction solids culture medium is therein together with being grown on
Coremium is separated according to being suitable to be placed in the shape of container from overall broth；
Detached culture medium and growth coremium therein are placed in container by step 2；
Solid medium is fixed on container bottom with supporter by step 3；
Step 4, notes wine in container；
Step 5, fixes more than 30 days, takes out supporter.
Further, the coremium described in step 1 is the coremium for growing to maturation.
Further, the solid medium area described in step 1 is defined by being put into container, Ke Yilve
Less than the area that can be entered needed for container, shape is not limited, and concrete shape is with attractive in appearance as principle.
Further, step 2 is placed in solid medium before wine holding container, by culture medium skiving, to protect
Card is grown on coremium therein and does not scatter and is defined；Further, culture medium skiving is less than to thickness
2cm；Further, culture medium skiving to thickness is less than into 1cm；Further, culture medium is cut
Thickness is as thin as less than 0.5cm.
Further, solid medium described in step 1 be with corn, crop stalk, crops cot,
Commodity trees branch or the culture medium that plant stem is main component；Further, the solid culture
The preferred grain culture medium of base；The corn is selected from Semen Tritici aestivi, Semen Maydiss, rice, Semen setariae, analysis for soybean powder, buckwheat
Any one or a few in wheat, Fructus Hordei Vulgaris, Herba bromi japonici, brown rice, Semen oryzae sativae.
Further, step 3 above support be wood, glass, rustless steel or other it is any not with base
Column construction made by the material that wine reacts, such as wooden stick, Glass rod, stainless steel bar etc.；Which is high
Degree is slightly less than container bottom to the height of vessel port.
Further, the height for wine being noted described in step 4 should be higher than that coremium；Further, note wine height
Higher than more than coremium 1cm.
Further, the set time described in step 5 is more than 35 days；Further, it is more than 45 days.
Further, step 5 continues immersion more than 60 days after taking out supporter；Further, continue
Immersion more than 80 days；Further, continue immersion 100 days.
The incubation of Cordyceps militaris (L.) Link. of the present invention is：Cordyceps militaris spawn is expanded by liquid culture
Increase；Strain after amplification carries out solid culture.The liquid culture and solid culture method can refer to existing
Cordyceps culturing method disclosed in technology.Liquid culture is, for the purpose of strain amplification, can to adopt routine
In strain propagation method, including slant culture, shake-flask culture, seed tank culture, fermentor cultivation
The combination of any one or a few method；Used medium is the conventional fluid medium in this area, such as
PSA culture medium or yeast extract powder-aminoacids complex-sucrose medium, yeast extract powder-white sugar-
Soy bean protein hydrolysate culture medium, wheat bran liquor-white sand sugar culture-medium etc.；More preferably PSA is trained
Foster base or yeast extract powder-aminoacids complex-sucrose medium；Further, PSA culture medium respectively into
Point ratio is：Rhizoma Solani tuber osi 15-25%, sucrose 1-5%, agar 1-5%；Yeast extract powder-compound ammonia
Base acid-each component ratio of sucrose medium is：Yeast extract powder 0.5-2%, aminoacids complex 0.2-1%,
Sucrose 2-5%.Further, each component ratio of PSA culture medium is：Rhizoma Solani tuber osi 20%, sucrose 2%,
Agar 2%；Yeast extract powder-each component ratio of aminoacids complex-sucrose medium is：Yeast extract powder
1%th, aminoacids complex 0.5%, sucrose 3.5%.During solid culture, in the mycelial growth stage
Using shading culture, cultivation temperature 22-24 DEG C, relative humidity are 65-70%；From coremium originate to
Harvest stages adopt illumination cultivation, and cultivation temperature is 20-22 DEG C, and relative humidity is 65-70%, falx
Ensure when beam is originated that intensity of illumination is 100-200Lx.
In the present invention, Cordyceps militaris (L.) Link. [Cordyceps militaris (Vuill.) Fr.] used is dispersed species.Cordyceps militaris (L.) Link.
It is distributed in the provinces and regions such as Hubei, Hebei, Jilin, Shaanxi, Yunnan, Guangxi, Guizhou, Sichuan, Taiwan.
Acquisition method according to general Chinese crude drug can all be adopted, and be strain known in the state of the art, it is possible to from business
Industry approach (such as edible fungi factory, institute) purchase is obtained.
The beneficial effects of the present invention is：
1. good looking appearance：Solid medium is together put into Sheng with coremium therein is grown on by the present invention
After wine container, with supporter supports, prevent coremium from floating, Jing is more than the month, treat oozing for coremium and wine
After flattening weighing apparatus thoroughly, supporter is removed, coremium and culture medium can stably stay in bottom of bottle, will not float,
Keep graceful situation.2. nutrient composition content is high：Cordyceps wine culture of the present invention 122 days or so, its
Adenosine content reaches 28.3 μ g/ml, is 1.77 times of the cordyceps wine that conventional method is prepared, and HEA is (i.e.
N6- (2- ethoxys) adenosine, also known as cocoon lavendustin, are the endemic elements of Cordycepses, as cordyceps product
One of quality control index) content reaches 54.12 μ g/ml, is the brewed Cordyceps militaris (L.) Link. of conventional method
1.87 times of wine.
Description of the drawings
Fig. 1 is used for the instrument for cutting solid medium
Cordyceps wine prepared by Fig. 2 the inventive method
The graph of a relation of adenosine content and time in Fig. 3 cordyceps wines
HEA contents and time chart in Fig. 4 cordyceps wines
The investigation of 1 set time of experimental example
1st, experimental technique
The Cordyceps militaris spawn of 1 amplification culture of Example, respectively using material listed by table 1 as solid culture
Base, carries out solid culture as described in Example 4, and the method according to embodiment 13 is harvested and consolidated
Fixed (every kind of culture medium experiment number is 20 bottles), periodically removes supporter, observation each group coremium and culture
The state of base.
2nd, experimental result
The results are shown in Table 1.
1 different set time culture medium of table and coremium state
As a result show, each solid medium can reach balance after fixing 30 days and no longer float.
Specific embodiment
1 liquid culture of embodiment
Slant culture：The strain for isolating and purifying is inoculated in slant tube, then the inclined-plane of strain will be inoculated with
Test tube is put into 22 DEG C of incubators, and incubation time is 7 days, treats that mycelia covers with test tube；
Shake-flask culture：Load 200m1 fluid mediums in 500m1 triangular flasks, in 0.11Mpa pressure
Lower autoclaving 30 minutes, is cooled to room temperature, and the strain of 1 slant tube is inoculated into 500m1
In triangular flask culture medium, constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, under the conditions of 150 revs/min are placed in
Culture, incubation time are 3 days；
Seed tank culture：Load 20L fluid mediums, culture medium temperature in 50L airlift fermentors
More than 95 DEG C, edible defoaming agent, addition for culture medium weight 0.03% is added to press in 0.11Mpa
Under power, it is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C,
4 bottles of above-mentioned cultured 500m1 shaking flasks strains are accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, ventilation
Measure as 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, it is right to reach
After number trophophase, whole volume of culture is 20L.
Fermentor cultivation：Load 200L fluid mediums, culture medium temperature in 500L airlift fermentors
Degree adds edible defoaming agent, addition for culture medium weight 0.03%, in 0.11Mpa more than 95 DEG C
Under pressure, it is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C,
Above-mentioned cultured 200L seed tanks seed is all accessed into culture, cultivation temperature is 22 ± 1 DEG C, ventilation
Measure as 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, it is right to reach
After number trophophase.Whole volume of culture is 200L.
Slant tube culture medium：Contain 200 grams of Rhizoma Solani tuber osi liquors, 20 grams of sucrose, 20 grams of fine jades per 1 liter of liquid
Fat, to 1000 milliliters, pH value is 6.5 for remaining moisturizing；
Fluid medium in shaking flask, seed tank and fermentation tank：Containing yeast extract powder 1%, compounded amino
Acid 0.5%, white sugar 3.5%, surplus moisturizing to 100%, pH value are 6.5.
2 liquid culture of embodiment
Slant culture：The strain for isolating and purifying is inoculated in slant tube, then the inclined-plane of strain will be inoculated with
Test tube is put into 22 DEG C of incubators, and incubation time is 7 days, treats that mycelia covers with test tube；
Shake-flask culture：Load 200m1 fluid mediums in 500m1 triangular flasks, in 0.11Mpa pressure
Lower autoclaving 30 minutes, is cooled to room temperature, and the strain of 1 slant tube is inoculated into 500m1
In triangular flask culture medium, constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, under the conditions of 150 revs/min are placed in
Culture, incubation time are 3 days；
Seed tank culture：Load 20L fluid mediums, culture medium temperature in 50L airlift fermentors
More than 95 DEG C, edible defoaming agent, addition for culture medium weight 0.03% is added to press in 0.11Mpa
Under power, it is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C,
4 bottles of above-mentioned cultured 500m1 shaking flasks strains are accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, ventilation
Measure as 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, it is right to reach
After number trophophase, whole volume of culture is 20L.
Slant tube culture medium：Contain 200 grams of Rhizoma Solani tuber osi liquors, 20 grams of sucrose, 20 grams of fine jades per 1 liter of liquid
Fat, to 1000 milliliters, pH value is 6.5 for remaining moisturizing；
Shaking flask and seed tank culture base：Contain 30 grams of yeast extract powders per 1 liter of liquid, 30 grams of white sugars,
5 grams of soy bean protein hydrolysates, to 1000 milliliters, pH value is 6.5 to the benefit that adds water.
3 liquid culture of embodiment
Slant culture：The strain for isolating and purifying is inoculated in slant tube, then the inclined-plane of strain will be inoculated with
Test tube is put into 22 DEG C of incubators, and incubation time is 7 days, treats that mycelia covers with test tube；
Seed tank culture：Load 20L fluid mediums, culture medium temperature in 50L gas-lifting type seed tanks
More than 95 DEG C, edible defoaming agent, addition for culture medium weight 0.03%, in 0.11Mpa are added
Under pressure, it is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, 20 DEG C are cooled to
When, the strain of 4 bottles of above-mentioned slant tubes is accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, and ventilation is
1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, reach logarithmic growth
After phase, whole volume of culture is 20L.
Fermentor cultivation：Load 200L fluid mediums, culture medium temperature in 500L airlift fermentors
Degree adds edible defoaming agent, addition for culture medium weight 0.03%, in 0.11Mpa more than 95 DEG C
Under pressure, it is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C,
Above-mentioned cultured 200L seed tanks seed is all accessed into culture, cultivation temperature is 22 ± 1 DEG C, ventilation
Measure as 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, it is right to reach
After number trophophase.Whole volume of culture is 200L.
Slant tube culture medium：Contain 200 grams of Rhizoma Solani tuber osi liquors, 20 grams of sucrose, 20 grams of fine jades per 1 liter of liquid
Fat, to 1000 milliliters, pH value is 6.5 for remaining moisturizing；
Seed tank and fermentation tank culture medium：Contain 40 grams of wheat bran liquors per 1 liter of liquid, 30 grams of white sugars,
To 1000 milliliters, pH value is 6.5 for remaining moisturizing.
4 solid culture of embodiment
Inoculation：Before inoculation culture vessel first in superclean bench or hundred grades of laminar flow hoods (under) use ultraviolet light
Irradiation 0.5h；1～3 Jing amplification culture of embodiment is obtained by 7% inoculum concentration by each culture vessel
Strain is inoculated on solid medium, and postvaccinal container is put into culturing room's culture.
Condition of culture：Culture room temperature is 22-24 DEG C, relative air humidity 65%-70%；First having to will
Culturing room's shading, makes a bacterium room substantially dark.When mycelium covers with charge level (indoor cultivation 3～5 days),
Proceed to illumination cultivation.Make regular check on after inoculation, reject growth potential difference, the culture vessel of bacteria infection in time.
Hereafter it is induced synthesis coremium period, keeps culture room temperature to be 20-22 DEG C, relative air humidity
65%～70%, it is ensured that have scattered light (100Lx～200Lx) induction coremium to be formed.Culturing room will fit
When ventilation, keep send out bacterium room air it is fresh.
5 solid medium of embodiment
After by Semen Tritici aestivi cleaning control water, appropriate water is added, its weight ratio is Semen Tritici aestivi:Water is 1：1.4, mixing
Uniformly；Load (lid punching above box, have ventilated membrane in pasting on hole) in the polypropylene box of sealing；
The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing,
Culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
6 solid medium of embodiment
After by rice cleaning control water, appropriate water is added, its weight ratio is rice:Water is 1：1.3, mixing
Uniformly；Load after mixing thoroughly in the polypropylene box of sealing and (punching is covered above box, in pasting on hole, has ventilative
Film)；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；Go out
After bacterium, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
7 solid medium of embodiment
After by brown rice cleaning control water, appropriate water is added, its weight ratio is brown rice：Water is 1：1.5, mix
Close uniform；Load after mixing thoroughly in the polypropylene box of sealing and (punching is covered above box, is had in pasting on hole
Air film)；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；
After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
8 solid medium of embodiment
After by Semen setariae cleaning control water, appropriate water is added, its weight ratio is Semen setariae:Water is 1：1.3, mixing
Uniformly；Load after mixing thoroughly in the polypropylene box of sealing and (punching is covered above box, in pasting on hole, has ventilative
Film)；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；Sterilizing
Afterwards, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
9 solid medium of embodiment
Corn cob 69%, cotton seed hullss 16%, wood flour 11%, Gypsum Fibrosum 3%, Calx 1%, solid material：
Water=1：1.5 (depending on feed moisture content) mixing is mixed thoroughly, is loaded in the polypropylene box of sealing after mixing thoroughly
(lid punching above box, have ventilated membrane in pasting on hole)；The culture vessel for installing culture medium is placed and is gone out
In bacterium pot, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cold
But it is indoor to less than 24 DEG C dislocation inoculations.
10 solid medium of embodiment
Agricultural crop straw culture medium：Corn stalk powder 50%, straw powder 20%, Cotton Gossypii straw 20%, analysis for soybean powder
5%th, Semen Maydis powder 5%, solid material：Water=1：1.5 (depending on feed moisture content) mixing is mixed thoroughly, is mixed
Load after even (lid punching above box, have ventilated membrane in pasting on hole) in the polypropylene box of sealing；Will
The culture vessel for installing culture medium is placed in autoclave, and sterilize at 121 DEG C 30-50min；After sterilizing, training
Foster container dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
11 solid medium of embodiment
Crops cot culture medium：Testa Tritici skin 35%, powdered rice hulls 20%, Rapeseed Shell powder 35%, analysis for soybean powder
5%th, Semen Maydis powder 5%, solid material：Water=1：1.5 (depending on feed moisture content) mixing is mixed thoroughly, is mixed
Load after even (lid punching above box, have ventilated membrane in pasting on hole) in the polypropylene box of sealing；Will
The culture vessel for installing culture medium is placed in autoclave, and sterilize at 121 DEG C 30-50min；After sterilizing, training
Foster container dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
12 solid medium of embodiment
Commodity trees branch or stalk culture medium：Ramulus Mori powder 70%, Ramulus ulmi pumilae powder 20%, analysis for soybean powder 5%,
Semen Maydis powder 5%, solid material：Water=1：1.5 (depending on feed moisture content) mixing is mixed thoroughly, after mixing thoroughly
Load (lid punching above box, have ventilated membrane in pasting on hole) in the polypropylene box of sealing；To install
The culture vessel of culture medium is placed in autoclave, and sterilize at 121 DEG C 30-50min；After sterilizing, culture is held
Device dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
13 coremium of embodiment is harvested and fixed
The strain of 1 Jing liquid cultures of embodiment is inoculated on wheat broth prepared by embodiment 5, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting.With special two diameter about slightly larger than bottleneck rustless steel garden cylinder (one end polishing is sharp,
See Fig. 1), coremium and solid medium are together cut down by edge perpendicular to the direction of culture medium,
Then the culture medium of bottom is cut off with hand papercutter, only remaining 0.5cm or so, this process can not touch disconnected spore
Stalk beam.Then coremium is placed in into wine jar center together with solid medium, coremium scatters to four directions,
Bottom of bottle is fixed in small bamboo rod.Slowly base liquor is poured into along bottle wall in the wine jar for fix culture medium
To coremium top 1-1.5cm was covered, the darkroom immersion after-ripening of cleaning is put into.After 25 days, remove and prop up
Support thing, now coremium and culture medium will not float again, obtain it is great sight cordyceps wine (see
Fig. 2).
14 coremium of embodiment is harvested and fixed
The strain of 2 Jing liquid cultures of embodiment is inoculated on rice medium prepared by embodiment 6, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting., with embodiment 13, difference is solid medium skiving to 1.0cm for harvesting and fixing meanss
Left and right, supporter is Glass rod；Remove supporter after 30 days, coremium and culture medium will not float again,
Obtain the cordyceps wine of great sight.
15 coremium of embodiment is harvested and fixed
The strain of 3 Jing liquid cultures of embodiment is inoculated in brown rice culture medium prepared by embodiment 7, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting., with embodiment 13, difference is solid medium skiving to 1.5cm for harvesting and fixing meanss
Left and right, supporter is stainless steel bar；Remove supporter after 30 days, coremium and culture medium will not be gone up again
It is floating, obtain the cordyceps wine of great sight.
16 coremium of embodiment is harvested and fixed
The strain of 1 Jing liquid cultures of embodiment is inoculated on grain culture medium prepared by embodiment 9, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting.Harvesting and fixing meanss remove supporter, coremium and training after 13,35 days with embodiment
Foster base will not float again, obtain the cordyceps wine of great sight.
17 coremium of embodiment is harvested and fixed
The strain of 2 Jing liquid cultures of embodiment is inoculated on straw medium prepared by embodiment 10, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting.Harvesting and fixing meanss remove supporter, coremium and training after 14,55 days with embodiment
Foster base will not float again, obtain the cordyceps wine of great sight.
18 coremium of embodiment is harvested and fixed
The strain of 3 Jing liquid cultures of embodiment is inoculated in cot culture medium prepared by embodiment 11, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting.Harvesting and fixing meanss are with embodiment 15.Remove supporter, coremium and culture within 60 days
Base will not float again, obtain the cordyceps wine of great sight.
19 coremium of embodiment is harvested and fixed
The strain of 1 Jing liquid cultures of embodiment is inoculated in stalk culture medium prepared by embodiment 12, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting.Harvesting and fixing meanss are with embodiment 13.Remove supporter, coremium and culture within 60 days
Base will not float again, obtain the cordyceps wine of great sight.
20 after-ripening of embodiment
13～19 cordyceps wine of embodiment is continued into immersion 45～100 days, goes out cellar for storing things, nutrition in Cordyceps militaris (L.) Link.
Composition is fully leached, obtain nutrient composition content it is high and it is great sight cordyceps wine.
The cordyceps wine active constituent content that 21 the inventive method of embodiment is prepared with prior art compares
The strain of 1 Jing liquid cultures of embodiment is inoculated on wheat broth prepared by embodiment 5, is pressed
Embodiment 4 carries out solid culture, selects vertical-growth, and growing way is uniform, and highly consistent coremium enters
Row harvesting；
Sample 1：Directly coremium is removed, is placed in wine jar, is prepared into cordyceps wine；
Sample 2：Harvested and fixed by 13 method of the embodiment of the present invention, be prepared into cordyceps wine；
Sample base liquor is ancient well 50 ° of wine of tribute.
In results of regular determination sample 1,2 adenosine and HEA content (adenosine assay method with reference to "《Adenosine
High-performance liquid chromatogram determination method》, Bai Hong chief editors《Health food effect assay method》The Chinese traditional Chinese medical science
Medicine publishing house, 2011, p214-215 "；The reference of HEA assay methods "《In Cordyceps militaris (L.) Link.sporophore
N6- (2- ethoxys)-adenosine is isolated and purified and antitumor action》, edible fungi journal 2013.20 (1)：
62～65 ").As a result Fig. 3～4 are seen.
Sample 1,2 adenosine contents reached highest and tended to be steady at 122 days or so as can be seen from Figure 3,
But 2 adenosine content of sample is apparently higher than sample 1；
Sample 1,2HEA contents reached highest and tended to be steady at 105 days or so as can be seen from Figure 4,
But HEA contents are apparently higher than sample 1 in sample 2.
From figs. 3 and 4 it can be seen that preparation method of the present invention is while appreciation effect is realized, can be as early as possible
Leach the effective ingredient in coremium, Fig. 3 and 4 can soak same time evident from going out, sample
2 active constituent content of product is apparently higher than than sample 1.
Claims (10)
1. a kind of preparation method of cordyceps wine attractive in appearance, it is characterised in that the method comprises the steps：
Step 1, it is in the Cordyceps militaris (L.) Link. solid culture stage, fraction solids culture medium is therein together with being grown on
Coremium is separated according to being suitable to be placed in the shape of container from overall broth；
Detached culture medium and growth coremium therein are placed in container by step 2；
Solid medium is fixed on container bottom with supporter by step 3；
Step 4, notes wine in container；With
Step 5, fixes more than 30 days, takes out supporter.
2. preparation method as claimed in claim 1, it is characterised in that the coremium described in step 1
To grow to the coremium of maturation.
3. preparation method as claimed in claim 1, it is characterised in that solid culture described in step 1
It is main with corn, crop stalk, crops cot, commodity trees branch or plant stem that base is
The culture medium of composition.
4. preparation method as claimed in claim 3, it is characterised in that the solid medium is paddy
Thing culture medium, the corn selected from Semen Tritici aestivi, Semen Maydiss, rice, Semen setariae, analysis for soybean powder, Semen Fagopyri Esculenti, Fructus Hordei Vulgaris,
Any one or a few in Herba bromi japonici, brown rice, Semen oryzae sativae.
5. preparation method as claimed in claim 1, it is characterised in that step 2 is by solid medium
Before being placed in wine holding container, culture medium skiving to thickness is less than into 2cm.
6. preparation method as claimed in claim 1, it is characterised in that step 3 above support is
Wood, glass, rustless steel or other column constructions made by any material not reacted with base liquor,
Which is highly slightly less than container bottom to the height of vessel port.
7. preparation method as claimed in claim 1, it is characterised in that set time described in step 5
For more than 35 days.
8. preparation method as claimed in claim 7, it is characterised in that set time described in step 5
For more than 45 days.
9. preparation method as claimed in claim 1, it is characterised in that after step 5 takes out supporter
Continue immersion more than 60 days.
10. preparation method as claimed in claim 9, it is characterised in that step 5 takes out supporter
Continue immersion more than 100 days afterwards.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107259329A (en) * | 2017-06-26 | 2017-10-20 | 江苏里下河地区农业科学研究所 | A kind of preparation method of the thick various grains gruel material of Cordyceps militaris |
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| WO2006021126A1 (en) * | 2004-07-09 | 2006-03-02 | Shaosheng Sun | Fresh living cordyceps wine |
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