CN106520887A - Aureomycin fermentation culture medium added with papain and application of culture medium - Google Patents
Aureomycin fermentation culture medium added with papain and application of culture medium Download PDFInfo
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Abstract
The invention discloses an aureomycin fermentation culture medium added with papain. Each 1L of the culture medium contains the following components: 12-25g of peanut meal, 5-15g of soybean cake powder, 50-100g of corn starch, 10-15g of corn steep liquor, 1-5g of yeast powder, 2.5-5g of soya-bean oil, 0.28-0.35g of papain, 0.1-0.2g of amylase, 1.0-3.5g of sodium chloride, 5.5-8g of ammonium sulfate, 5.6-8.0g of calcium carbonate and 0.1-0.3g of magnesium sulfate. By adding the papain to an aureomycin fermentation formula, the use of a large amount of raw materials in the antibiotic fermentation industry is reduced; and meanwhile, a phenomenon of a large amount of waste caused by incomplete utilization of the raw materials in the fermentation process is avoided as well.
Description
Technical field
The invention belongs to antibiotic fermentation field, and in particular to a kind of Ferment of DM culture medium of addition papain
And its application.
Background technology
Chlortetracycline biological fermentation process mainly adopts modern biotechnology fermentation engineering, is formed sediment by the Semen Maydiss in fermentation substrate
The raw material culture streptomyces aureus such as powder, peanut cake powder, soybean cake powder, inorganic salt so as to which metabolism produces chlortetracycline.Corn starch is made
For primary carbon source, it is the indispensable composition of cell growth and metabolism；Peanut cake powder, soybean cake powder, yeast powder etc. are used as main
Nitrogen source, the synthesis for cell growth and nitrogenous metabolites provide long-acting nitrogen source, the vitamin, life also required for offer cell growth
Long factor etc..But, in natural sweat, these raw material practical efficiencies are very low, and main cause is exactly：At present
It is small peptide by the material protein enzymolysis in base material that the fermentation of antibiotic relies primarily on the enzyme of microorganism itself secretion in sweat
Or aminoacid, but the enzyme amount of microorganism itself secretion is limited, and enzymolysis efficiency is relatively low, on the one hand, cause wastage of material with
And the fermentation period of antibiotic is extended, raw material input cost and time input cost are increased, on the other hand, is seriously limited
A large amount of synthesis of antibiotic.Therefore, in the epoch that Future is in short supply, how effectively to improve the enzyme of these natural materials
Solution efficiency, the real utilization rate for improving these raw materials, is only the competitive emphases of following antibiotic fermentation industry.
Papain（Papain）, also known as carase, it is a kind of proteolytic enzyme.Under acid, neutral, alkaline environment
Equal energy decomposing protein, it is a kind of containing sulfydryl (- SH) endopeptidase, and the activity with protease and esterase has wide
Specificity, have stronger hydrolysis ability to animal and vegetable protein, polypeptide, ester, amide etc..It has that enzyme activity is high, heat stability is good,
The features such as natural safe and sanitary, therefore be used widely in industries such as food, medicine, feedstuff, daily use chemicals, leather and weavings.
104263712 A of patent CN describes a kind of composite pawpaw glycoprotein protease, is made up of following weight parts raw material：Fructus Chaenomeliss
Protease 50-70 parts, Ginger Protease 20-40 parts, bromelain 5-15 parts, there is provided composite pawpaw glycoprotein protease is clear in medicated beer
Clearly, meat softening, cookiess pineization, prepare antitumor, antiangiogenic property leukemia, anti-microbial pathogen, anti-parasite medicine, whitening skin
Skin, it is anti-ageing wait for a long time in application.The Compound papain composition that 102719422 A of patent CN is provided, in traditional Fructus Chaenomeliss egg
Oligomeric xylose is added in white enzyme, the activity of papain is improve, patent additionally provides Compound papain composition
Application in medical industry, food industry, beauty industry, chemical product industry, feed industry and leather industry.
Report with regard to papain is substantially all and is based on how to carry out papain compounding to improve wood at present
Melon protease activity and expand its range of application；And papain is in biological fermentation field especially Ferment of DM side
Face has no related application report.
The content of the invention
It is an object of the invention to provide a kind of stable Ferment of DM level of production of energy, the utilization rate for improving raw material, drop
The Ferment of DM culture medium of the addition papain of the input cost of raw material and its application in low production.
For achieving the above object, the technical solution used in the present invention scheme is：A kind of chlortetracycline of addition papain
Fermentation medium, every 1 L culture medium（Aqueous solution）In concrete composition be：12 ~ 25g of peanut cake powder, 5 ~ 15g of soybean cake powder,
50 ~ 100g of corn starch, 10 ~ 15g of Semen Maydis pulp, 1 ~ 5g of yeast powder, 2.5 ~ 5g of Oleum Glycines, 0.28 ~ 0.35g of papain, starch
0.1 ~ 0.2g of enzyme, 1.0 ~ 3.5g of Sodium Chloride, 5.5 ~ 8g of ammonium sulfate, 5.6 ~ 8.0g of Calcium Carbonate, 0.1 ~ 0.3g of magnesium sulfate.
Papain Enzymatic Activity >=the 400000U/mL.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane for 3 ~ 5 days by inclined-plane mycelia culture
Mycelia；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 26 ~ 33 DEG C, 110
～140 revs/min of 20～26h of bottom fermentation prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 26 ~ 33
DEG C, 110～140 revs/min of 4～8h of bottom fermentation prepare secondary seed solutions；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 30-35 DEG C of condition bottom fermentation
90-124 h（Culture Stress control is controlled in 50-120cp in 0.01Mpa-0.04Mpa, fermentation culture viscosity）It is as golden mould
Plain fermentation liquid.
The screening of streptomyces aureus spore and slant preservation are this area very conventional technique means, less go to live in the household of one's in-laws on getting married herein
State.The Ferment of DM culture medium of the addition papain in the present invention is in step（3）In fermentation tank in use.
The beneficial effect comprise that：By adding papain, the addition of organic nitrogen source is reduced, raising has
The utilization rate of machine nitrogen source, in comparing existing fermentating formula, yeast powder reduces ratio 50～60%（W/W）Or soybean cake powder is reduced
Ratio 50%（W/W）, and other proportioning raw materials it is constant when, moreover it is possible to make chlortetracycline（CTC）Fermentation yield slightly improve；Reduce
Raw material input cost in industrial fermentation production, and avoid raw material to a certain extent and be not fully utilized and cause
Wasting phenomenon, with Important Economic and environmental benefit.
Specific embodiment
By the following examples the present invention is illustrated, but protection scope of the present invention is not limited thereto.
Papain Enzymatic Activity >=400000U/mL used in following examples.
Embodiment 1
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
20g, soybean cake powder 10g, corn starch 75g, Semen Maydis pulp 12g, yeast powder 5g, Oleum Glycines 4g, papain 0.28g, amylase
0.15g, Sodium Chloride 2.0g, ammonium sulfate 6.5g, Calcium Carbonate 6.6g, magnesium sulfate 0.2g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 4 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 30 DEG C, 120 turns/
Bottom fermentation 24h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 30 DEG C, 120 revs/min issue
Ferment 5h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 30 DEG C of condition bottom fermentations
100h is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 1 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 1
Reference examples 1 with the difference of embodiment 1 are：In fermentation medium without papain, soybean cake powder adds
Dosage is 20g.
The Aureomycin fermentation liquor that reference examples 1 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 2
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
20g, soybean cake powder 12g, corn starch 50g, Semen Maydis pulp 15g, yeast powder 3g, Oleum Glycines 5g, papain 0.28g, amylase
0.2g, Sodium Chloride 1.0g, ammonium sulfate 8g, Calcium Carbonate 5.6g, magnesium sulfate 0.1g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 5 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 26 DEG C, 140 turns/
Bottom fermentation 26h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 26 DEG C, 140 revs/min issue
Ferment 8h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 30 DEG C of condition bottom fermentations 90
H is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 2 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 2
Reference examples 2 with the difference of embodiment 2 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 7.5g.
The Aureomycin fermentation liquor that reference examples 2 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 3
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
24g, soybean cake powder 5g, corn starch 100g, Semen Maydis pulp 10g, yeast powder 4g, Oleum Glycines 5g, papain 0.34g, amylase
0.2g, Sodium Chloride 3.5g, ammonium sulfate 5.5g, Calcium Carbonate 8.0g, magnesium sulfate 0.1g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 3 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 33 DEG C, 110 turns/
Bottom fermentation 20h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 33 DEG C, 110 revs/min issue
Ferment 4h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 33 DEG C of condition bottom fermentation 90-
114 h are Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 3 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 3
Reference examples 3 with the difference of embodiment 3 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 8g.
The Aureomycin fermentation liquor that reference examples 3 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 4
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
12g, soybean cake powder 15g, corn starch 80g, Semen Maydis pulp 12g, yeast powder 5g, Oleum Glycines 3.5g, papain 0.32g, starch
Enzyme 0.15g, Sodium Chloride 2.0g, ammonium sulfate 7g, Calcium Carbonate 7.0g, magnesium sulfate 0.15g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 5 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 32 DEG C, 140 turns/
Bottom fermentation 22h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 32 DEG C, 140 revs/min issue
Ferment 4h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 32 DEG C of condition bottom fermentations 95
H is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 4 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 4
Reference examples 4 with the difference of embodiment 4 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 10g.
The Aureomycin fermentation liquor that reference examples 4 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 5
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
12g, soybean cake powder 10g, corn starch 80g, Semen Maydis pulp 13g, yeast powder 5g, Oleum Glycines 4.5g, papain 0.32g, starch
Enzyme 0.13g, Sodium Chloride 3.1g, ammonium sulfate 7.5g, Calcium Carbonate 7.6g, magnesium sulfate 0.18g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 3 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 26 DEG C, 140 turns/
Bottom fermentation 26h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 26 DEG C, 140 revs/min issue
Ferment 8h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 30 DEG C of condition bottom fermentations 124
H is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 5 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 5
Reference examples 5 with the difference of embodiment 5 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 12g.
The Aureomycin fermentation liquor that reference examples 5 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 6
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
15g, soybean cake powder 5g, corn starch 75g, Semen Maydis pulp 10g, yeast powder 5g, Oleum Glycines 5g, papain 0.30g, amylase
0.1g, Sodium Chloride 2.0g, ammonium sulfate 5.5g, Calcium Carbonate 8.0g, magnesium sulfate 0.1g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 4 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 28 DEG C, 120 turns/
Bottom fermentation 24h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 28 DEG C, 120 revs/min issue
Ferment 6h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 31 DEG C of condition bottom fermentations 94
H is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 6 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 6
Reference examples 6 with the difference of embodiment 6 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 12.5g.
The Aureomycin fermentation liquor that reference examples 6 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 7
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
20g, soybean cake powder 10g, corn starch 90g, Semen Maydis pulp 10g, yeast powder 3g, Oleum Glycines 2.5g, papain 0.33g, starch
Enzyme 0.1g, Sodium Chloride 1.0g, ammonium sulfate 8g, Calcium Carbonate 5.6g, magnesium sulfate 0.1g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 5 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 31 DEG C, 130 turns/
Bottom fermentation 22h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 31 DEG C, 130 revs/min issue
4～8h of ferment prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 32 DEG C of condition bottom fermentations 94
H is Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 7 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 7
Reference examples 7 with the difference of embodiment 7 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 6g.
The Aureomycin fermentation liquor that reference examples 7 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 8
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
25g, soybean cake powder 15g, corn starch 85g, Semen Maydis pulp 10g, yeast powder 1g, Oleum Glycines 5g, papain 0.33g, amylase
0.15g, Sodium Chloride 2.5g, ammonium sulfate 6.5g, Calcium Carbonate 7.6g, magnesium sulfate 0.23g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 5 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 32 DEG C, 125 turns/
Bottom fermentation 24h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 32 DEG C, 125 revs/min issue
Ferment 4h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 35 DEG C of condition bottom fermentation 90h
As Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 8 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 8
Reference examples 8 with the difference of embodiment 8 are：In fermentation medium without papain, soybean cake powder adds
Dosage is 30g.
The Aureomycin fermentation liquor that reference examples 8 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
Embodiment 9
A kind of Ferment of DM culture medium of addition papain, the concrete composition in every 1 L culture medium is：Peanut cake powder
20g, soybean cake powder 5g, corn starch 85g, Semen Maydis pulp 12g, yeast powder 5g, Oleum Glycines 4.5g, papain 0.30g, starch
Enzyme 0.18g, Sodium Chloride 2.5g, ammonium sulfate 6.8g, Calcium Carbonate 6.9g, magnesium sulfate 0.19g.
The Ferment of DM culture medium is applied in Ferment of DM production, specifically includes following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane bacterium for 5 days by inclined-plane mycelia culture
Silk；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 33 DEG C, 110 turns/
Bottom fermentation 26h is divided to prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 33 DEG C, 110 revs/min issue
Ferment 5h prepares secondary seed solution；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 35 DEG C of condition bottom fermentation 90h
As Aureomycin fermentation liquor（50m3）.
The Aureomycin fermentation liquor that embodiment 9 is obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7312 kg.
Reference examples 9
Reference examples 9 with the difference of embodiment 9 are：Without papain in fermentation medium, the addition of yeast powder
Measure as 10g.
The Aureomycin fermentation liquor that reference examples 9 are obtained is sent to refinement workshop and is extracted, and the yield of 15%CTC is 7282 kg.
From above-described embodiment, the present invention by adding papain, reduces organic nitrogen source in fermentating formula
Addition, improves the utilization rate of organic nitrogen source, the additional proportion of the papain for using for machine nitrogen source addition 0.8～
1.2%（W/W）, it is 50～60% that in fermentating formula, yeast powder reduces ratio（W/W）, or soybean cake powder reduce ratio be 50%（W/
W）, when other proportioning raw materials are constant, moreover it is possible to which the fermentation yield of chlortetracycline CTC is slightly improved.
Analyzed by data above, the present invention reduces the throwing of organic nitrogen source in fermentating formula by adding papain
Enter amount, not only do not reduce the fermentation yield of chlortetracycline CTC, and CTC yield is slightly improved relative to control in the present invention；Jing
Cross revenue accounting（Only consider cost of material income）：Papain is added according to best proportion（Additional proportion adds for machine nitrogen source
The 1.0% of dosage（W/W））, by 4980 yuan/ton of existing market price soybean cake powder, papain unit price is about organic nitrogen source
300000 yuan/ton, the 12100 yuan/ton of calculating of Angel Yeast powder can reduce by 330～674 yuan of raw material input cost per tank.
The invention provides application of the papain during Ferment of DM, by Ferment of DM formula
Addition papain, reduces a large amount of uses of the antibiotic fermentation industry to raw material, while it also avoid sweat Central Plains
A large amount of wasting phenomenons that material is not fully utilized and causes.
Claims (4)
1. a kind of Ferment of DM culture medium of addition papain, it is characterised in that containing as follows in every 1 L culture medium
Composition：12 ~ 25g of peanut cake powder, 5 ~ 15g of soybean cake powder, 50 ~ 100g of corn starch, 10 ~ 15g of Semen Maydis pulp, 1 ~ 5g of yeast powder,
2.5 ~ 5g of Oleum Glycines, 0.28 ~ 0.35g of papain, 0.1 ~ 0.2g of amylase, 1.0 ~ 3.5g of Sodium Chloride, ammonium sulfate 5.5 ~
8g, 5.6 ~ 8.0g of Calcium Carbonate, 0.1 ~ 0.3g of magnesium sulfate.
2. Ferment of DM culture medium as claimed in claim 1, it is characterised in that：The Papain Enzymatic Activity >=
400000U/mL。
3. applied in Ferment of DM production using Ferment of DM culture medium described in claim 1 or 2.
4. application as claimed in claim 3, it is characterised in that specifically include following steps：
（1）The streptomyces aureus spore of slant preservation, 32 ± 1 DEG C of cultures are prepared female bottle inclined-plane for 3 ~ 5 days by inclined-plane mycelia culture
Mycelia；
（2）Prepared by secondary seed solution, by step（1）Middle female bottle outer inclined-plane mycelium inoculation in first class seed pot, 26 ~ 33 DEG C, 110
～140 revs/min of 20～26h of bottom fermentation prepare primary seed solution；Primary seed solution is inoculated in secondary seed tank, 26 ~ 33
DEG C, 110～140 revs/min of 4～8h of bottom fermentation prepare secondary seed solutions；
（3）Aureomycin fermentation liquor is prepared, by step（2）Middle secondary seed solution is inoculated in fermentation tank, 30-35 DEG C of condition bottom fermentation
90-124 h are Aureomycin fermentation liquor.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110923157A (en) * | 2020-01-15 | 2020-03-27 | 齐鲁工业大学 | Preparation of culture medium for producing glutathione and glutathione separation method |
| CN112680495A (en) * | 2021-01-15 | 2021-04-20 | 驻马店华中正大有限公司 | Method for improving utilization rate of aureomycin fermentation tank raw materials |
| CN113519837A (en) * | 2021-07-24 | 2021-10-22 | 湖北真福医药有限公司 | Thrombolytic enzyme product capable of improving alcoholism and preparation method and application thereof |
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| CN110923157A (en) * | 2020-01-15 | 2020-03-27 | 齐鲁工业大学 | Preparation of culture medium for producing glutathione and glutathione separation method |
| CN110923157B (en) * | 2020-01-15 | 2022-04-15 | 齐鲁工业大学 | Preparation of culture medium for producing glutathione and glutathione separation method |
| CN112680495A (en) * | 2021-01-15 | 2021-04-20 | 驻马店华中正大有限公司 | Method for improving utilization rate of aureomycin fermentation tank raw materials |
| CN113519837A (en) * | 2021-07-24 | 2021-10-22 | 湖北真福医药有限公司 | Thrombolytic enzyme product capable of improving alcoholism and preparation method and application thereof |
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