CN106520638B - A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment - Google Patents
A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment Download PDFInfo
- Publication number
- CN106520638B CN106520638B CN201611225401.2A CN201611225401A CN106520638B CN 106520638 B CN106520638 B CN 106520638B CN 201611225401 A CN201611225401 A CN 201611225401A CN 106520638 B CN106520638 B CN 106520638B
- Authority
- CN
- China
- Prior art keywords
- powdery mildew
- pseudomonas fluorescens
- treatment
- prevention
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to functional microorganism applied technical field, a kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment is specifically provided.The Pseudomonas fluorescens, deposit number are CCTCC NO:M 2014274, and particularly significant to the inhibitory effect of monofilament shell powdery mildew, antibacterial circle diameter reaches 28 mm, can effectively prevent the powdery mildew of cucumber, strawberry and tobacco.Preparation containing fluorescent pseudomonas microbial provided by the invention can be developed into biological pesticide, dedicated for the prevention and treatment of plant powdery mildew, have a extensive future.
Description
Technical field
The present invention relates to plant disease technical fields, and in particular to a kind of Pseudomonas fluorescens is anti-in plant powdery mildew
Application in controlling.
Background technique
It is increasing with floristic, the continuous diversification of plant culture mode, plant disease is on the rise, and passes
There is many drawbacks for the chemical prevention and control method of system, such as: a large amount of uses of chemical pesticide cause serious environmental pollution, agriculture
A series of pairs of people's lives nocuousness such as the pesticide residue of product is also increasingly severe, and germ drug resistance generation speed is also getting faster
Influence.Low-carbon economy is advocated, in environmental protection and conservation society now, seeks that human lives' fertile new technology again can be protected
Very adapt to the development of today's society.
Biological control be using it is a kind of biology or its product tackle another biology method, be divided into worm control worm, with
Bacterium controls worm, fungus treatment three categories.The correlation between biological species is utilized in it, with a kind of or a kind of biology or the suppression of its product
System another kind or another kind of biology.The method of biological control has very much, and microbial control is exactly one of.Microbial control master
If anti-by interbiotic Competition, antibiosis, parasitization, bacteriolysis and induction using beneficial microorganism
Property etc., to inhibit the survival and activity of certain pathogens, it is advantageous in that resourceful, selective strong, noresidue, without dirt
Dye, it is at low cost, it is simultaneous prevent it is simultaneous control, increasing both production and income, keep ecological balance, play long-acting effect.
Many kinds of, widely applied mainly fungi and the bacterium in production of biocontrol microorganisms.Biocontrol fungi mainly includes wood
Mould, the viscous broom of mould, cupreum, Penicillium notatum, Verticillium chlamydosporiun and mycorrhizal fungi, shield shell is mould etc., it is known that Trichoderma at least
Can parasitism 18 belong to 29 kinds of phytopathogens, such as Sclerotinia, Helminthosporium sp. category, Fusarium, Armillaria, colletotrichum, silk core
Pseudomonas, Verticillium, Venturia；Cupreum has degraded cellulose, organic matter characteristic, in consideration of it, it is widely used in planting
In terms of the biological control of object pathogen, wherein chaetomium globosum, angle cupreum can preferably prevent sugarcane samping off, and
The generation of tomato wilt and apple scab is preferably prevented and treated, Rhizoctonia solani, Fusarium oxysporum have inhibiting effect, this
Outside, chaetomium globosum can also preferably protect cereal, oat and barley from by Fusarium nivale and the mould caused children of standing grain rotation spore
Seedling is withered.Biocontrol bacteria generally comprises bacillus, actinoplanes, Pseudomonas, Xanthomonas, streptomycete
Category, Agrobacterium, Alcaligenes, Arthrobacter, azotobacter, Erwinia, Flavobacterium, micromonospora and root nodule
Pseudomonas.Wherein bacillus subtilis C-22 is to Colletotrichum gossypii, wilt, rhizoctonia solani, verticillium wilt pathogen, cotton phytophthora root rot
Bacterium and cotton leaf spot fungi have good inhibiting effect；Bacillus subtilis XG-1 has the growth of withered germ of water-melon mycelia
Stronger inhibiting effect, meanwhile, bacillus subtilis Henu11 has good inhibiting effect to Rhizoctonia cerealis.
Currently, biocontrol microorganisms are deposited under field conditions (factors) in control of plant disease, colonization ability is poor, medicine resistance ability is weak and prevention and treatment
The problems such as effect is unstable, therefore the microorganism fungus kind that further screenability is excellent, stability is good is used for controlling plant diseases,
It is research emphasis in the art at present.
Summary of the invention
The present invention is to solve prior art problem, provides a kind of method of controlling plant diseases.The method is to pass through
Plant leaf surface application containing Pseudomonas fluorescens (Pseudomonas fluorescens) microorganism formulation, effectively inhibit dislike
Seedling diseases and powdery mildew, significant effect.
One aspect of the present invention provides a kind of microorganism formulation, be by by Pseudomonas fluorescens (Pseudomonas fluorescens) carry out what fermentation was prepared.
The preferred Pseudomonas fluorescens HF-3(of Pseudomonas fluorescensPseudomonas fluorescens HF-3),
It is preserved in the China typical culture collection center of Wuhan, China Wuhan University on June 24th, 2014, deposit number is
CCTCC NO: M 2014274。
The preparation method of the microorganism formulation, includes the following steps:
Pseudomonas fluorescens is seeded in the LB liquid medium in shaking flask, 28~30 DEG C, revolving speed be 150rpm condition
Lower culture 18h obtains liquid fermentation seed；Liquid fermentation seed is inoculated into the fermentor equipped with liquid fermentation medium,
28~30 DEG C, inoculum concentration 5~10%, fermentor 130~180rpm of mixing speed, under conditions of ventilatory capacity 5~10%, fermentation
24~48h to get arrive the microorganism formulation containing Pseudomonas fluorescens.
Each component and its content are respectively as follows: 30 g/L of maltose, 3 g/L of glucose, ferment in the liquid fermentation medium
Female 5 g/L of cream, ammonium sulfate 4.5 g/L, K2HPO40.5 g/L, MgSO4.7H2O 0.05g/L, CaCl2 0.02 g/L, NaCl
0.15 g/L。
One aspect of the present invention provides a kind of method of controlling plant diseases, is to include by above-mentioned in plant leaf surface application
What the microorganism formulation of Pseudomonas fluorescens was realized.
The plant disease is powdery mildew.
The microorganism formulation is liquid preparation.
The viable count of Pseudomonas fluorescens is more than 10 in the microorganism formulation9CFU/mL。
The method of the controlling plant diseases, specifically: the above-mentioned microorganism formulation comprising Pseudomonas fluorescens is diluted
0-900 times, by 10-15mL/m2Dosage the microorganism formulation containing Pseudomonas fluorescens is uniformly sprayed on plant leaf surface；7-
It is sprayed one time by same amount again after 10 days；It is used in conjunction 3-5 times.
Preparation containing fluorescent pseudomonas microbial provided by the invention pads shell to Fusarium oxysporum, Rhizoctonia solani Kuhn, white rot
The inhibitory effect of spore bacterium and pink single citri ending bacteria is unobvious, but it is particularly significant to the inhibitory effect of monofilament shell powdery mildew, antibacterial
Loop diameter reaches 28 mm, can effectively prevent plant powdery mildew.After the microorganism formulation dilutes 100 times, to powdery mildew of cucumber
Prevention and treatment efficiency is up to 95.8%；When being diluted to 900 times, the prevention and treatment efficiency of powdery mildew of strawberry is remained to be higher than 85%, effect is very aobvious
It writes；After 300 times of dilution, the prevention and treatment efficiency to Powdery Mildew in Tobacco is more than 80%, suitable with the prevention and treatment efficiency of chemicals control group,
Unexpected technical results have been achieved.Preparation containing fluorescent pseudomonas microbial provided by the invention can be developed into biological agriculture
Medicine has a extensive future dedicated for the prevention and treatment of plant powdery mildew.
Specific embodiment
The selection of Pseudomonas fluorescens described in embodiment of the present invention Pseudomonas fluorescens (Pseudomonas fluorescens) HF-3, the China typical culture collection of Wuhan, China Wuhan University is preserved on June 24th, 2014
Center, deposit number are CCTCC NO:M 2014274.The bacterial strain screening is from Yantai east ocean group salmon recirculated water
In water sample in cultivating wastewater purification tower.Show the nitrite nitrogen studies have found that in the bacterial strain energy efficient degradation breeding wastewater, tool
There is the effect of purifying water body.
Applicant is further discovered that the bacterial strain also has significant application effect in terms of control of plant disease in the present invention.
The present invention is further explained combined with specific embodiments below.For specific method or material used in embodiment
Material, those skilled in the art can carry out conventional replacement according to existing technology and select on the basis of the technology of the present invention thinking
It selects, is not limited solely to the specific record of the embodiment of the present invention.
The preparation method of 1 preparation containing fluorescent pseudomonas microbial of embodiment
1, prepared by seed liquor
Pseudomonas fluorescens HF-3 is seeded in the LB liquid medium in shaking flask, 28~30 DEG C, revolving speed 150rpm
Under the conditions of cultivate 18h, obtain liquid fermentation seed；
2, fermentation process:
Liquid fermentation seed is inoculated by 5~10% inoculum concentration equipped with liquid fermentation medium (each group in culture medium
Divide and its content is respectively as follows: 30 g/L of maltose, 3 g/L of glucose, 5 g/L of yeast extract, ammonium sulfate 4.5 g/L, K2HPO4
0.5 g/L, MgSO4.7H2O 0.05g/L, CaCl2 0.15 g/L of 0.02 g/L, NaCl) fermentor in, 28~30 DEG C,
130~180rpm, under conditions of ventilatory capacity 5~10%, the 24~48h that ferments is to get the microorganism system containing Pseudomonas fluorescens of arriving
Agent.Wherein, the viable bacteria amount of Pseudomonas fluorescens is about 109CFU/mL。
2 bacteriostatic experiment of embodiment
(1) prepared by pathogen:
By Fusarium oxysporum, Rhizoctonia solani Kuhn, Coniella diplodiella bacterium, pink single citri ending bacteria and monofilament shell powdery mildew (by blueness
Island agriculture university gives) fungus block (7 mm of Φ) be connected to PDA solid medium center, 30 DEG C of 7 d of culture respectively；
(2) punching diffusion method screening:
PDA culture medium temperature after sterilizing is maintained at 55 DEG C, the oblique of above-mentioned pathogen is then gently rinsed with sterile water
Face obtains pathogen spore suspension, and takes and be separately added into PDA culture medium in right amount, inverted plate after mixing.After to be solidified, obtain
Cause of disease bacterial content is about 107The PDA solid medium of cfu/ml.
It is respectively made a hole (7 mm of Φ) with Oxford cup in the symmetrical two sides away from 25 mm of culture dish center, and it is real that 150 μ l are added
Microorganism formulation that example 1 is prepared is applied in hole, every kind of pathogen set 3 it is parallel.Plate is put into 30 DEG C of incubators, 72 h
The diameter of inhibition zone is measured afterwards.It the results are shown in Table 1.
Inhibitory effect of the 1 Pseudomonas fluorescens HF-3 of table to pathogen
| Pathogen | Fusarium oxysporum | Rhizoctonia solani Kuhn | Coniella diplodiella bacterium | Pink single citri ending bacteria | Monofilament shell powdery mildew |
| Antibacterial circle diameter | 11mm | 9mm | 14mm | 11mm | 28mm |
It can be seen that the microorganism formulation of the HF-3 provided by the invention containing Pseudomonas fluorescens to sharp spore from the data of table 1
Sickle-like bacteria, Rhizoctonia solani Kuhn, the inhibitory effect of Coniella diplodiella bacterium and pink single citri ending bacteria are unobvious, but it is to monofilament shell white powder
The inhibitory effect of bacterium is particularly significant, and antibacterial circle diameter reaches 28 mm, and unexpected technical results have been achieved.
Application of 3 preparation containing fluorescent pseudomonas microbial of embodiment in powdery mildew of cucumber prevention and treatment
1, place: Qingdao City, the town Xi Fu, Chengyang District green house of vegetables is tested；
2, experimental setup:
1) experimental group: setting three processing groups, and each processing group is randomly selected two ridge cucumber, and totally 100, each processing group
Between setting protection row.
In the growth medium of cucumber, the microorganism formulation that embodiment 1 is prepared is diluted with water 100,300,500 respectively
After times, by 10mL/m2Dosage uniformly spray on the throughout cucumber leaves of reason group；It is sprayed one time by same amount again after 7 days；
It is used in conjunction 3 times；After last time has sprayed bacterium solution 10 days, the incidence of each processing group powdery mildew of cucumber is checked.
2) control group: cucumber leaves are sprayed using clear water, other operations are all the same with experimental group.
3, interpretation of result
Disease scale standard: 0 grade of disease-free spot；1 grade: lesion area accounts for 1/4 or less leaf area；2 grades: lesion area
Account for the 1/4-2/4 of leaf area；3: lesion area accounts for the 2/4-3/4 of leaf area；4 grades: lesion area accounts for 3/4 of leaf area or more.
Disease index=[∑ (the sick grade value × grade morbidity number of sheets)/(investigation total number of sheets × highest disease grade value)] × 100%
Calculation formula=[1- (disease index/control disease index of processing)] × 100% for preventing and treating efficiency.
Experimental data is analyzed with SPSS statistical software, the results are shown in Table 2:
Control efficiency of 2 preparation containing fluorescent pseudomonas microbial of table to powdery mildew of cucumber
Can be seen that the microorganism formulation provided by the invention containing Pseudomonas fluorescens from the data of table 2 can effectively prevent
Powdery mildew of cucumber after the microorganism formulation dilutes 100 times, is up to 95.8% to the prevention and treatment efficiency of powdery mildew of cucumber, dilution 500
After times, prevention and treatment efficiency is still higher than 85%, and unexpected technical results have been achieved.The microorganism formulation can be used as biological prevention and control agent,
It is widely used in the prevention and treatment of powdery mildew of cucumber.
Application of 4 preparation containing fluorescent pseudomonas microbial of embodiment in prevention and treatment powdery mildew of strawberry
1, place: Qingdao City Laoshan District strawberry withered peach strawberry cultivating base is tested；
2, experimental setup:
1) experimental group: three processing groups, each 10 ridge strawberry of processing group, set up guarantor between each processing group by totally 1200
Shield row；
It comes into bloom in strawberry, preparation containing fluorescent pseudomonas microbial made from embodiment is diluted with water to 300 respectively,
600, after 900 times, by 15mL/m2Dosage be uniformly sprayed at Strawberry Leaves anyway on face and melon stem；Sprinkling interval 10 days every time,
It is used in conjunction 3 times；After last time has sprayed bacterium solution 10 days, the incidence of powdery mildew of strawberry is checked.
2) control group: Strawberry Leaves are sprayed using clear water, other operations are all the same with experimental group.
3, interpretation of result
Experimental data is analyzed using method same as Example 3, the results are shown in Table 3:
Control efficiency of 3 preparation containing fluorescent pseudomonas microbial of table to powdery mildew of strawberry
From the experimental result of table 3 it is found that preparation containing fluorescent pseudomonas microbial provided by the invention can also effectively prevent
Powdery mildew of strawberry, when the microorganism formulation is diluted to 900 times, prevention and treatment efficiency of the microorganism formulation to powdery mildew of strawberry
It remains to be higher than 85%, effect highly significant.
Application of 5 preparation containing fluorescent pseudomonas microbial of embodiment in Powdery Mildew in Tobacco prevention and treatment
1, place: the tested greenhouse of the Chinese Academy of Agricultural Sciences tobacco is tested；
2, experimental setup:
1) experimental group: setting 3 processing groups, and each processing group is randomly selected two ridge tobacco, and totally 100, each processing group
Between setting protection row.
In the growth medium of tobacco, the microorganism formulation that embodiment 1 is prepared is diluted with water 100,300 and respectively
After 500 times, by 10mL/m2Dosage uniformly spray on the throughout tobacco leaf of reason group；Again by same amount sprinkling one after 7 days
Time；It is used in conjunction 5 times；After last time has sprayed bacterium solution 10 days, the incidence of each processing group Powdery Mildew in Tobacco is checked.
2) chemicals control group: using 2% antimycoin aqua, 200 times of dilutions, other operations are homogeneous with the above method
Together.
3) blank control group: cucumber leaves are sprayed using clear water, other operations are all the same with experimental group.
3, interpretation of result
Experimental data is analyzed using method same as Example 3, the results are shown in Table 4:
Control efficiency of 4 preparation containing fluorescent pseudomonas microbial of table to Powdery Mildew in Tobacco
It can be seen that the microorganism formulation provided by the invention containing Pseudomonas fluorescens to tobacco white powder from the data of table 4
Disease also has significant control efficiency.After the microorganism formulation dilutes 300 times, the prevention and treatment efficiency to Powdery Mildew in Tobacco is more than
80%, suitable with the prevention and treatment efficiency of chemicals control group, after 500 times of dilution, prevention and treatment efficiency is still higher than 75%, achieves expectation not
The technical effect arrived.
To sum up, preparation containing fluorescent pseudomonas microbial provided by the invention can be developed into biological pesticide, dedicated for planting
The prevention and treatment of object powdery mildew, has a extensive future.
Claims (3)
1. a kind of method of controlling plant diseases, which is characterized in that the method is to include by applying on plant leaf surface
What the microorganism formulation of Pseudomonas fluorescens (Pseudomonas fluorescens) was realized, the Pseudomonas fluorescens
Deposit number is CCTCC NO:M 2014274；The plant disease is powdery mildew of cucumber, powdery mildew of strawberry and tobacco white powder
Disease.
2. the method as described in claim 1, which is characterized in that the microorganism formulation is liquid preparation.
3. method according to claim 1 or 2, which is characterized in that the method specifically: the microorganism formulation is dilute
Release 0-900 times；By 10-15mL/m2Dosage microorganism formulation is uniformly sprayed on plant leaf surface；Again by identical after 7-10 days
Dosage is sprayed one time；It is used in conjunction 3-5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611225401.2A CN106520638B (en) | 2016-12-27 | 2016-12-27 | A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611225401.2A CN106520638B (en) | 2016-12-27 | 2016-12-27 | A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106520638A CN106520638A (en) | 2017-03-22 |
| CN106520638B true CN106520638B (en) | 2019-08-16 |
Family
ID=58338920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611225401.2A Active CN106520638B (en) | 2016-12-27 | 2016-12-27 | A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106520638B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502584B (en) * | 2017-10-16 | 2019-10-25 | 山东省林业科学研究院 | One fluorescent pseudomonads and its application in prevention and treatment walnut black spot |
| CN109251871B (en) * | 2018-07-11 | 2020-07-10 | 华中农业大学 | Pseudomonas fluorescens and application thereof |
| CN111184029B (en) * | 2019-12-10 | 2021-03-23 | 中国农业科学院烟草研究所 | Application of pseudomonas fluorescens in prevention and removal of dicotyledonous weeds and powdery mildew, and microbial inoculum, preparation method and use method thereof |
| CN112715582A (en) * | 2020-12-29 | 2021-04-30 | 南京启佑生物科技有限公司 | Preparation method and application of pesticide special for strawberries |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005289944A (en) * | 2004-04-05 | 2005-10-20 | Mitsui Chemicals Inc | Agent and method for controlling powdery mildew with pseudomonas fluorescens |
| JP2005330184A (en) * | 2004-05-18 | 2005-12-02 | Mitsui Chemicals Inc | Method for controlling plant terrestrial disease injury with biological agrochemical containing bacterium as control microorganism |
| CN104164389A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Pseudomonas fluorescens and application thereof in aquaculture |
-
2016
- 2016-12-27 CN CN201611225401.2A patent/CN106520638B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005289944A (en) * | 2004-04-05 | 2005-10-20 | Mitsui Chemicals Inc | Agent and method for controlling powdery mildew with pseudomonas fluorescens |
| JP2005330184A (en) * | 2004-05-18 | 2005-12-02 | Mitsui Chemicals Inc | Method for controlling plant terrestrial disease injury with biological agrochemical containing bacterium as control microorganism |
| CN104164389A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Pseudomonas fluorescens and application thereof in aquaculture |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106520638A (en) | 2017-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108102957B (en) | Bacillus subtilis capable of solubilizing phosphorus and producing polyglutamic acid at high yield and application thereof | |
| Khan et al. | Field testing of antagonists of Fusarium head blight incited by Gibberella zeae | |
| CN104560837B (en) | A kind of bacillus amyloliquefaciens and its application | |
| CN105733990B (en) | One plant of Paenibacillus polymyxa and its application in control of plant disease | |
| CN106520638B (en) | A kind of application of Pseudomonas fluorescens in plant powdery mildew prevention and treatment | |
| CN110713951B (en) | Pantoea agglomerans strain and application thereof | |
| CN104673705B (en) | One plant prevent and treat wheat scab bacillus amyloliquefaciens and its application | |
| CN114369550B (en) | Bacillus amyloliquefaciens for promoting oat growth and application thereof | |
| CN112940991B (en) | Antibacterial activity of Siamese bacillus and application thereof | |
| KR101677513B1 (en) | Paenibacillus polymyxa strain effective against Root Rot Pathogen of ginseng and Use Thereof | |
| CN113667620A (en) | Bacillus subtilis Bs1218m and its application | |
| CN110317747A (en) | A kind of bacillus amyloliquefaciens JT68 and its application in prevention and treatment tea anthracnose | |
| CN101518264B (en) | Microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof | |
| CN107629985B (en) | Plant endophytic bacterium with antagonistic effect on plant pathogenic fungi | |
| KR20080045346A (en) | Bacillus subtilis m27 and biological control of sclerotinia rot by using the same | |
| CN107828697B (en) | Paenibacillus polymyxa biocontrol strain AF01 and application thereof | |
| CN106342900B (en) | A kind of Streptomyces jingyangensis wettable powder and preparation method thereof | |
| CN104152372A (en) | Biocontrol strain G68 for preventing and treating plant diseases, and preparation method and application of microbial inoculant thereof | |
| CN116790422A (en) | Siamese bacillus and application thereof | |
| KR101139794B1 (en) | Biological control of plant diseases by mixed Bacillus subtilis M27 and 5M43 | |
| CN116855407A (en) | Streptomyces ka Wu Ershi Y274 and application thereof | |
| CN114946885B (en) | Microbial pesticide for resisting leaf spot disease of polygonatum odoratum | |
| CN114958665B (en) | Bacillus saxifragus S21 for preventing and treating melon bacterial fruit blotch and application thereof | |
| CN115975875A (en) | Bacillus bailii with biocontrol effect, microbial inoculum, preparation method and application | |
| CN112553129B (en) | Bacillus belgii LN9-2 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |