CN106513707B - A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis - Google Patents
A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis Download PDFInfo
- Publication number
- CN106513707B CN106513707B CN201611115101.9A CN201611115101A CN106513707B CN 106513707 B CN106513707 B CN 106513707B CN 201611115101 A CN201611115101 A CN 201611115101A CN 106513707 B CN106513707 B CN 106513707B
- Authority
- CN
- China
- Prior art keywords
- blueberry
- nano silver
- leaf extract
- reflux
- biosynthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of nano silver antibacterial agent using blueberry leaf extract biosynthesis and its preparation process, belong to antiseptic technical field.Including following technique：1）, stock acquisition blueberry blade, clean after drying to constant weight, obtain dry blueberry blade；2）, prepare blueberry extract dry blueberry blade is shredded, be then placed in reflux and be heated to reflux together with extractant, then natural cooling, filtering carry out centrifugal treating, supernatant is taken to obtain blueberry leaf extract；3）, by AgNO3Solution and distilled water are mixed to prepare mixed liquor, add after blueberry leaf extract stirs evenly and carry out being heated to reflux handling, and stop being heated to reflux after solution becomes golden yellow to get to the nano silver antibacterial agent of biosynthesis.
Description
Technical field
The present invention relates to a kind of nano silver antibacterial agent using blueberry leaf extract biosynthesis and its preparation process, belong to
In antiseptic technical field.
Background technology
People carry out sterilizing using silver-colored series bactericidal agent long history, and silver element or silver ion can be incorporated in
The surface of microbial cell film changes its permeability, and then is combined with endobacillary albumen and nucleic acid, influences bacterium division and increases
It grows, eventually leads to its death.In recent years, with the fast development of nanometer technology, nano material because its unique quantum effect,
Small-size effect and big specific surface area and show the excellent specific property different from conventional material, people is caused widely to study emerging
Interest.Nano silver (Silver nanoparticles, AgNPs) is the simple substance under the nm regime prepared using nanometer technology
Silver, bactericidal effect promotion further than common inorganic silver fungicide, has a broad antifungal spectrum, antibacterial activity are strong, and are not likely to produce
Drug resistance, the research and development for antiseptic of new generation open new direction, have wide development prospect.
Tradition prepare nano silver mainly by physics, chemistry the methods of, but physical method is to equipment requirement height, and chemistry side
The chemical reagent that method uses again damages to environmental and human health impacts.In addition, Nano silver grain prepared by the above method is easily
Oxidation is reunited, and limits its popularization in practical applications.In recent years, people begin attempt to utilize the organisms such as plant, microorganism
The active material inside contained prepares nano silver as reducing agent and stabilizer, compared with traditional nano silver preparation method, nanometer
Silver-colored biological synthesis process is not required to add any reducing agent, cheap, and reaction condition is mild, environmentally protective, and combined coefficient is high, is conducive to
Industrialization amplification.In addition, rich in large biological molecules such as protein, lipid, polysaccharide in bio-extract, it can be with nano material knot
It closes and assigns its unique physicochemical character and biological characteristics, make product more stabilization, fungistatic effect more notable.Therefore, nanometer
Biological synthesis process becomes the new hot spot in nanometer synthesis field, has wide research and development prospect.
Blueberry (Vaccinium Spp.), it is perennial small berries shrub, belongs to Ericaceae, Vaccinium.Originating from north
U.S., worldwide plantation extensively, there is a kind more than 450 at present.Contain abundant nutritional ingredient in the fruit of blueberry,
Mainly there are anthocyanidin, catechuic acid, flavonols, flavanols, chlorogenic acid, tannic acid, superoxide dismutase, ascorbic acid, amino
The ingredients such as acid and trace element.Blueberry have it is anti-oxidant, prevent cranial nerve aging, be protection eyesight, prevention of cardiovascular disease, anti-
Aging, enhancing immunity of organisms, anticancer and other effects.Therefore, blueberry is classified as five big healthy food of the mankind by FAO (Food and Agriculture Organization of the United Nation)
One of.Blueberry leaf is the byproduct of blueberry plantation, is largely abandoned on end of the branch after picking fruit.It is however, many in recent years
Research shows that the antioxidant contents such as chlorogenic acid, polyphenols in blueberry leaf maintain an equal level with the content in fruit, are even higher.Cause
This, blueberry leaf can be used as the substitute of ingredient in Blueberry.
Invention content
In place of solving above-mentioned the shortcomings of the prior art, provide a kind of useless using blueberry picking
Gurry is raw material, is reducing agent using the abundant antioxidant in blueberry leaf, and low production cost reacts mild environmental protection, energy
The environmentally hazardous drawback of conventional chemical synthesis is enough avoided, and generated time is short, production efficiency utilizes blueberry leaf extract
The nano silver antibacterial agent of biosynthesis and its preparation process.
The present invention a kind of nano silver antibacterial agent using blueberry leaf extract biosynthesis preparation process, it is special it
It is in including the following steps：
1st, it stocks up
Blueberry blade is acquired, drying to constant weight after cleaning, and obtains dry blueberry blade；
The blueberry blade is first rinsed with water totally, then is washed 2-5 times with distilled water；
The blueberry blade, which is placed in baking oven, dries, and the temperature in baking oven is controlled at 50-70 DEG C；
2nd, blueberry extract is prepared
Dry blueberry blade is shredded, is placed in reflux and is heated to reflux together with extractant, dry
Blueberry blade is with extractant according to mass ratio 1:（2-100）Ratio mixing, product natural cooling, mistake after being heated to reflux
Filter, then carries out centrifugal treating, supernatant is taken to obtain blueberry leaf extract, is saved backup；
The extractant is any one in distilled water, ethyl alcohol, ether；
The temperature being heated to reflux is controlled at 40-100 DEG C, is heated to reflux 0.5-12 hours；
The centrifugal treating carries out in centrifuge, and the rotating speed of centrifuge is controlled at 5000-10000 revs/min, centrifuges 10-
15 minutes；
Described save backup is kept in dark place in the environment of 0-10 DEG C；
3rd, using blueberry leaf extract as reducing agent one-step method biosynthesis nano silver
By AgNO3Solution is 1 ﹕ according to volume ratio with distilled water（1-1000）Ratio be mixed to prepare mixed liquor, add
Blueberry leaf extract carries out being heated to reflux handling after stirring evenly, wherein, the volume ratio of blueberry leaf extract and mixed liquor
For 1 ﹕（1-100）, stop being heated to reflux, to get to the nano silver antibacterial agent of biosynthesis, being protected from light after solution becomes golden yellow
It preserves；
The AgNO3A concentration of 0.01-1mol/L of solution；
The temperature being heated to reflux is controlled at 40-100 DEG C, is heated to reflux 0.5-12 hours；
The temperature being kept in dark place is controlled at 0-10 DEG C.
The present invention utilizes the nano silver antibacterial agent of blueberry leaf extract biosynthesis, and the raw material blueberry leaf used is blueberries
Waste after real picking, can be made full use of, synthetic method low production cost of the present invention, instead by the present invention
Should be mild environmentally friendly, the environmentally hazardous drawback of conventional chemical synthesis can be avoided, generated time is short, and production efficiency is high, antibacterial reality
It tests and shows the biosynthesis nano silver antibacterial agent of blueberry leaf to Vibrio anguillarum, vibrio alginolyticus, vibrio parahemolyticus, dotted gas unit cell etc. 4
The inhibition zone size of kind aquatic pathogenic bacterium is respectively 12.7,14.4,13.9 and 11.9mm, wherein being handled with this product of 20 μ g/mL
Bacteriostasis rate is up to 99.2 % after Vibrio anguillarum 4 hours.Illustrating that the bacteriostatic agent is wide to aquatic pathogenic bacterium antimicrobial spectrum, fungistatic effect is notable,
It is had broad application prospects in aquaculture field.
Fig. 1：Utilize the ultravioletvisible absorption collection of illustrative plates of the nano silver antibacterial agent of blueberry leaf extract biosynthesis；
Fig. 2：Utilize the transmission electron microscope photo of the nano silver antibacterial agent of blueberry leaf extract biosynthesis；
Fig. 3：Using the nano silver antibacterial agent of blueberry leaf extract biosynthesis to the Bactericidal test of Vibrio anguillarum；
Fig. 4：Using the nano silver antibacterial agent of blueberry leaf extract biosynthesis to the Bactericidal test of vibrio alginolyticus；
Fig. 5：It is real to the inhibition zone of vibrio parahemolyticus using the nano silver antibacterial agent of blueberry leaf extract biosynthesis
It tests；
Fig. 6：It is real to the inhibition zone of Aeromonas punctata using the nano silver antibacterial agent of blueberry leaf extract biosynthesis
It tests；
Fig. 7：The nano silver antibacterial agent of the blueberry leaf extract biosynthesis of various concentration is to the antibacterial power of Vibrio anguillarum
Learn curve.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiments obtained under the premise of no creative work, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of preparation process of the nano silver antibacterial agent using blueberry leaf extract biosynthesis of the present embodiment, including with
Lower preparation process：
（1）Material prepares：
The northern high clump blueberry of acquisition (Vaccinium corymbosumL.) blade is first rinsed well with tap water, then is used
Distilled water washs 3 times, is placed in baking oven, and 60 DEG C of drying obtain dry blueberry blade.
（2）The preparation of blueberry extract：
5.0g blueberry blades are weighed with assay balance, blade is shredded, 10 mL extractants are added in, in Soxhlet at 40 DEG C
It is heated to reflux in extractor 12 hours, solution natural cooling obtained is filtered with filter paper, then 5000 rpm centrifugations 15
Minute, supernatant is taken to obtain blueberry leaf extract, 4 DEG C save backup.
（3）Using blueberry leaf extract as reducing agent one-step method biosynthesis nano silver：
10 mL distilled waters are taken in 100 mL round-bottomed flasks, add in the AgNO of 10 mL, 0.01 mol/L3Solution, then add
Enter the above-mentioned blueberry leaf extracts of 20 mL, stir evenly, 20 DEG C are heated to reflux about 12 hours, treat that solution gradually becomes golden yellow
When stop heating to get to the nano silver antibacterial agent of biosynthesis, 4 DEG C are kept in dark place.
Embodiment 2
A kind of preparation process of the nano silver antibacterial agent using blueberry leaf extract biosynthesis of the present embodiment, including with
Lower preparation process：
（1）Material prepares：
Acquire short clump blueberry (Vaccinium angustifoliumL.) blade is first rinsed well with tap water, then
It is washed 5 times, is placed in baking oven with distilled water, 70 DEG C of drying obtain dry blueberry blade；
（2）The preparation of blueberry extract：
5.0g blueberry blades are weighed with assay balance, blade is shredded, 500 mL extractants are added in, in rope at 100 DEG C
It is heated to reflux in family name's extractor 0.5 hour, by solution natural cooling obtained, 10000 rpm are centrifuged 10 minutes, take supernatant i.e.
Blueberry leaf extract is obtained, 10 DEG C save backup；
（3）Using blueberry leaf extract as reducing agent one-step method biosynthesis nano silver：
60 mL distilled waters are taken in 100 mL round-bottomed flasks, add in the AgNO of 60 μ L, 1 mol/L3Solution adds 0.5
The above-mentioned blueberry leaf extracts of mL, stir evenly, and 80 DEG C are heated to reflux about 10 hours, stop when solution gradually becomes golden yellow
To get to the nano silver material of biosynthesis, 4 DEG C are kept in dark place for heating.
Embodiment 3
（1）Material prepares：
The northern high clump blueberry of acquisition (Vaccinium corymbosumL.) blade is first rinsed well with tap water, then is used
Distilled water washs 3 times, is placed in baking oven, and 60 DEG C of drying obtain dry blueberry blade.
（2）The preparation of blueberry extract：
5.0g blueberry blades are weighed with assay balance, blade is shredded, 100 mL extractants are added in, in Soxhlet at 90 DEG C
It is heated to reflux in extractor 1 hour, solution natural cooling obtained is filtered, then with Whatman No.1 qualitative filter papers
5000 rpm are centrifuged 10 minutes, supernatant are taken to obtain blueberry leaf extract, 4 DEG C save backup.
（3）Using blueberry leaf extract as reducing agent one-step method biosynthesis nano silver：
20 mL distilled waters are taken in 100 mL round-bottomed flasks, add in the AgNO of 200 μ L, 0.1 mol/L3Solution adds
The above-mentioned blueberry leaf extracts of 2 mL, stir evenly, and 90 DEG C are heated to reflux about 1 hour, stop when solution gradually becomes golden yellow
It only heats to get to the nano silver antibacterial agent of biosynthesis, 4 DEG C are kept in dark place.
Embodiment 4
The embodiment the step of（3）Carry out following step again later
（4）Ultraviolet-visible spectrophotometer detects：
The absorption maximum of the Nano silver solution of biosynthesis obtained using ultraviolet-visible spectrophotometer detecting step 3
Wavelength, scanning wavelength ranging from 200-800 nm.From attached drawing 1 it can be seen that Nano silver solution occurs one near 425 nm
A apparent absorption peak, and typical Nano silver grain in the range of 400-500 nm since characteristic surface plasma is total to
It shakes and generates characteristic absorption peak, it is possible thereby to illustrate blueberry leaf extract successfully by AgNO3It is reduced into nano-Ag particles.
（5）Transmission electron microscope detects：
The nano silver of biosynthesis that 5-10 μ L steps 3 obtain is taken to be added dropwise on copper mesh, drying at room temperature uses transmitted electron
Microscope observes the form and grain size of nano-Ag particles under 80-300 kV voltages, from attached drawing 2 it can be seen that biosynthesis is received
Rice Argent grain is spherical shape, and nano particle diameter size is 10-30 nm, and grain size is relatively uniform.
The nano silver antibacterial agent of preparation is detected into its bacteriostatic activity with the following two kinds bacteriostatic experiment：
（6）Bactericidal test：
4 kinds of aquatic pathogenic bacteriums are selected respectively（Vibrio anguillarum, vibrio alginolyticus, vibrio parahemolyticus, dotted gas unit cell）As finger
Show bacterium, using the bacteriostatic activity of conventional cylinder plate method detection biosynthesis nano silver antibacterial agent.
For examination marine fishery pathogen using 2216E culture mediums (peptone 5g, yeast extract 1 g, high ferric phosphate 0.01g,
Chen Haishui is settled to 1000 mL), 28 DEG C of cultures.It takes and is added in 5 mL fluid nutrient mediums for examination 50 μ L of bacterium, 150 rpm concussions 8-
10 h, until increased logarithmic phase, inoculum normal saline dilution bacterial concentration to 106CFU/mL takes 100 μ L uniformly to apply
It is distributed on 2216E solid mediums, Oxford cup is then placed on culture medium（Cup outer diameter 8mm）, add in biosynthesis nano silver
Culture dish is separately placed in 28 DEG C of perseverances by 20 μ L of bacteriostatic agent with physiological saline and each 20 μ L of blueberry leaf extract as control
Overnight incubation in warm incubator observes the generation of inhibition zone around Oxford cup.The experimental results showed that blueberry leaf extract is in itself
With certain bacteriostatic activity, only there is the fungistatic effect of part to the aquatic pathogenic bacterium in Oxford cup（C in attached drawing 3-6）；With
Blueberry leaf extract synthesis nano silver then to it is all for examination aquatic pathogenic bacteriums generate significant inhibition zone, to Vibrio anguillarum,
The inhibition zone size of 4 kinds of aquatic pathogenic bacteriums such as vibrio alginolyticus, vibrio parahemolyticus and dotted gas unit cell is respectively 12.7,14.4,
13.9 and 11.9 mm（A in attached drawing 3-6）；And physiological saline is then to for trying aquatic pathogenic bacterium completely without inhibiting effect（Attached drawing
B in 3-6）.The above results show the nano silver that the active material in blueberry blade can be effectively synthesized, and to the nano silver of generation
With collaboration inhibitory effect, its higher bacteriostatic activity is assigned.
（7）Antibacterial dynamic experiment：
Using Vibrio anguillarum as indicator bacteria, by Vibrio anguillarum 28 DEG C in 2216E fluid nutrient mediums, 150 r/min are cultivated to logarithm
Cultured bacterium solution is diluted to 1 × 10 by the rise period6CFU/mL.Dilution bacterium solution is dispensed into three test tubes, wherein two
Test tube is separately added into different amounts of biosynthesis nano silver antibacterial agent（Final concentration is respectively 10,20 μ g/mL）, another test tube
Physiological saline is added in as control, 150 r/min in 28 DEG C of constant-temperature tables is placed in and cultivates, every 0,10,30,60,120,180,
240 min are sampled, with absorbance value of the spectrophotometric measurement inoculum under 600 nm（OD600）, draw bacterial growth and move
Force diagram detects the influence that our product grows Vibrio anguillarum.By attached drawing 7 it can be seen that the nano silver of 10 μ g/mL is handled
Group can postpone the time that Vibrio anguillarum reaches increased logarithmic phase；The nano silver of 20 μ g/mL can generate Vibrio anguillarum strong suppression
It makes and uses, with the extension of incubation time, the OD values of this group of bacterium solution illustrate that bacterium therein does not almost have almost without changing
There is any proliferation phenomenon；And the bacterium in saline control group can reach rapidly increased logarithmic phase, thalline number obtains soon
Speed increases.This product of 10 μ g/mL and 20 μ g/mL is respectively to the bacteriostasis rate of Vibrio anguillarum after 4 hours knowable to being computed
55.6% and 99.2%.Above-mentioned experiment shows that the nano silver of leaf of Fructus vaccini vitis-idaeae extract biosynthesis can significantly inhibit water in water environment
The growth of pathogen is produced, is laid a good foundation to application of the novel nanometer silver antibacterial material in aquaculture.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (6)
1. the preparation process of a kind of nano silver antibacterial agent using blueberry leaf extract biosynthesis, it is characterised in that including following
Step：
1）, stock
Blueberry blade is acquired, drying to constant weight after cleaning, and obtains dry blueberry blade；
2）, prepare blueberry extract
Dry blueberry blade is shredded, is then placed in reflux and is heated to reflux together with extractant, dry
Blueberry blade is with extractant according to mass ratio 1：（2-100）Ratio mixing, product natural cooling, mistake after being heated to reflux
Filter, then carries out centrifugal treating, supernatant is taken to obtain blueberry leaf extract, be kept in dark place spare；
3）, using blueberry leaf extract as reducing agent one-step method biosynthesis nano silver
By AgNO3Solution is 1 ﹕ according to volume ratio with distilled water（1-1000）Ratio be mixed to prepare mixed liquor, add blueberry leaf
Piece extracting solution carries out being heated to reflux handling after stirring evenly, wherein, the volume ratio of blueberry leaf extract and mixed liquor is 1：（1-
100）, stop being heated to reflux, to get to the nano silver antibacterial agent of biosynthesis, being kept in dark place after solution becomes golden yellow；
Step 2）Described in centrifugal treating carried out in centrifuge, the rotating speed of centrifuge is controlled at 5000-10000 revs/min, from
Heart 10-15 minutes；
Step 3）Described in the temperature that is heated to reflux control at 90 DEG C, be heated to reflux 1 hour；
Step 3）Described in the temperature that is kept in dark place control at 0-6 DEG C；
Step 2）Described in extractant be distilled water.
2. a kind of preparation work of nano silver antibacterial agent using blueberry leaf extract biosynthesis described in accordance with the claim 1
Skill, it is characterised in that step 1）Described in blueberry blade be first rinsed with water totally, then washed 2-5 times with distilled water.
3. a kind of preparation work of nano silver antibacterial agent using blueberry leaf extract biosynthesis described in accordance with the claim 1
Skill, it is characterised in that step 1）Described in blueberry blade be placed in baking oven and dry, the temperature in baking oven is controlled at 50-70 DEG C.
4. a kind of preparation work of nano silver antibacterial agent using blueberry leaf extract biosynthesis described in accordance with the claim 1
Skill, it is characterised in that step 2）Described in the temperature that is heated to reflux control at 20-100 DEG C, be heated to reflux 0.5-12 hour.
5. a kind of preparation work of nano silver antibacterial agent using blueberry leaf extract biosynthesis described in accordance with the claim 1
Skill, it is characterised in that step 2）Described in save backup preserved in the environment of 0-6 DEG C.
6. a kind of preparation work of nano silver antibacterial agent using blueberry leaf extract biosynthesis described in accordance with the claim 1
Skill, it is characterised in that step 3）Described in AgNO3A concentration of 0.01-1mol/L of solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611115101.9A CN106513707B (en) | 2016-12-07 | 2016-12-07 | A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611115101.9A CN106513707B (en) | 2016-12-07 | 2016-12-07 | A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106513707A CN106513707A (en) | 2017-03-22 |
| CN106513707B true CN106513707B (en) | 2018-06-08 |
Family
ID=58342578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611115101.9A Active CN106513707B (en) | 2016-12-07 | 2016-12-07 | A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106513707B (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107309438B (en) * | 2017-07-14 | 2021-03-09 | 福建农林大学 | Method for preparing nano-silver composite particles from phyllanthus emblica polysaccharide |
| CN107671305B (en) * | 2017-09-22 | 2019-05-10 | 鲁东大学 | The method for quickly preparing nano silver antibacterial agent using Ligustrum quihoui fruit extracting solution |
| CN107683893B (en) * | 2017-09-22 | 2021-01-01 | 鲁东大学 | Preparation method of aquatic product bacteriostatic agent |
| CN109909512A (en) * | 2017-12-12 | 2019-06-21 | 中国科学院深圳先进技术研究院 | A kind of silver nanowires and its green magnanimity preparation method |
| CN109158613A (en) * | 2018-08-29 | 2019-01-08 | 扬州大学 | A method of utilizing sinigrin synthesizing nano-silver |
| CN109702218B (en) * | 2018-12-25 | 2020-09-29 | 浙江大学 | Method for preparing nano-silver particles by using phyllanthus emblica extract, product and application |
| CN109528768B (en) * | 2018-12-29 | 2021-04-27 | 鲁东大学 | Nano bacteriostatic preparation for treating equine endometritis and preparation method thereof |
| US10500645B1 (en) | 2019-01-07 | 2019-12-10 | King Saud University | Method of synthesizing silver nanoparticles using mint extract |
| CN109909513B (en) * | 2019-04-26 | 2022-03-04 | 中国科学院西双版纳热带植物园 | Method for biologically synthesizing nano silver particles by using glochidion pubescens and application |
| CN111097921B (en) * | 2020-01-13 | 2021-05-14 | 山西大学 | Anti-colon cancer silver nanoparticles and preparation method thereof |
| CN111184024A (en) * | 2020-01-14 | 2020-05-22 | 鲁东大学 | Method for preparing nano-silver composite bacteriostatic agent by using thyme leaf extracting solution |
| CN111296506B (en) * | 2020-01-14 | 2021-10-22 | 鲁东大学 | Method for preparing efficient bacteriostatic agent by using reed leaf extract |
| CN111202092B (en) * | 2020-01-14 | 2021-05-18 | 鲁东大学 | Method for preparing efficient and stable composite nano-silver bacteriostatic agent by one-step method through lotus root extract |
| CN113996801B (en) * | 2021-10-28 | 2023-01-31 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on red amaranth extracting solution |
| CN114523121B (en) * | 2022-02-09 | 2024-04-05 | 上海市农业技术推广服务中心 | Method for preparing nano silver particles by using eggplant leaf extract, product and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958198A (en) * | 2006-11-28 | 2007-05-09 | 厦门大学 | Plant reduction method for preparing silver Nano granules and gold Nano granules |
| CN101498037A (en) * | 2009-01-14 | 2009-08-05 | 厦门大学 | Method for preparing single crystal silver nano-wire by plant biomass reduction |
| CN101905331A (en) * | 2010-08-24 | 2010-12-08 | 厦门大学 | Method for extracting nano-silver in aqueous phase by using ionic liquid |
| CN101912976A (en) * | 2010-08-24 | 2010-12-15 | 厦门大学 | Method for preparing silver nano granules by reducing plant extract |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2878401B1 (en) * | 2013-11-27 | 2018-07-11 | King Saud University | Method for preparing noble metal nanoparticles |
-
2016
- 2016-12-07 CN CN201611115101.9A patent/CN106513707B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958198A (en) * | 2006-11-28 | 2007-05-09 | 厦门大学 | Plant reduction method for preparing silver Nano granules and gold Nano granules |
| CN101498037A (en) * | 2009-01-14 | 2009-08-05 | 厦门大学 | Method for preparing single crystal silver nano-wire by plant biomass reduction |
| CN101905331A (en) * | 2010-08-24 | 2010-12-08 | 厦门大学 | Method for extracting nano-silver in aqueous phase by using ionic liquid |
| CN101912976A (en) * | 2010-08-24 | 2010-12-15 | 厦门大学 | Method for preparing silver nano granules by reducing plant extract |
Non-Patent Citations (1)
| Title |
|---|
| 蓝莓叶提取液的抑菌作用及其在鲜切竹笋中的应用研究;胡筱;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20150715(第07期);B024-125 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106513707A (en) | 2017-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106513707B (en) | A kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis | |
| Velmurugan et al. | Extraction of natural colorant from purple sweet potato and dyeing of fabrics with silver nanoparticles for augmented antibacterial activity against skin pathogens | |
| Huang et al. | Continuous-release beads of natural allelochemicals for the long-term control of cyanobacterial growth: Preparation, release dynamics and inhibitory effects | |
| CN101518265A (en) | Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention | |
| CN114316320A (en) | Degradable pH-responsive intelligent packaging material and preparation method thereof | |
| CN107602521A (en) | A kind of method and obtained OPC and purposes of pitaya peel extraction OPC | |
| CN109939051A (en) | A kind of mulberries fermentation magma and its preparation method and application | |
| CN107157801A (en) | Containing natural Radix Salviae Miltiorrhizae polysaccharide compound antibacterial hand sanitizer and preparation method thereof | |
| CN111202092B (en) | Method for preparing efficient and stable composite nano-silver bacteriostatic agent by one-step method through lotus root extract | |
| CN101463339B (en) | Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof | |
| CN109207548A (en) | A kind of peanut coat oligomeric proanthocyanidins, preparation method and application | |
| CN110063366A (en) | A kind of preparation method of lacquer tree fat composite nano silver liquid antibiotic coating antistaling agent | |
| CN101892156A (en) | Rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease | |
| CN109908060A (en) | A kind of raspberry fermentation magma and its preparation method and application | |
| CN103555616B (en) | One bacillus amyloliquefaciens and the application of liquid preparation in control walnut root rot thereof | |
| CN109702218A (en) | A kind of method and product and application preparing nano-Ag particles using Phyllanthus embical fruit extracting solution | |
| CN105901681A (en) | Preparation methods for extract liquid, fermentation extracellular liquid and fermentation intracellular liquid of dictyophora, and application thereof | |
| CN117158440A (en) | Application of turmeric carbon nanodots in prevention and treatment of wheat scab | |
| CN103773690A (en) | Open-type culture method of microalgae | |
| CN108795827A (en) | A kind of bacillus megaterium BM22 and its water dispersible granules and application | |
| CN106821935A (en) | The method that bacteria inhibiting composition prepares cosmetics is extracted from TAL waste material | |
| Tin et al. | Effect of preparation and extraction parameters of banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities | |
| CN107671305B (en) | The method for quickly preparing nano silver antibacterial agent using Ligustrum quihoui fruit extracting solution | |
| CN103875667B (en) | A kind of antioxidant and application thereof | |
| CN109880750A (en) | A kind of application of the method for improving Thelephora ganbajun mycelium and polysaccharide yield and wizened granulose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to a nano silver bacteriostatic agent synthesized by blueberry leaf extract and a preparation process thereof Effective date of registration: 20211216 Granted publication date: 20180608 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220111 Address after: 165000 Daxing'anling e-commerce Industrial Park, Guanghui Road, Shuguang community, gagdaqi District, Daxing'anling Prefecture, Heilongjiang Province Patentee after: Heilongjiang Yuanye pastoral agricultural industry development Co.,Ltd. Address before: 264002 No. 186, Hongqi Middle Road, Zhifu District, Yantai City, Shandong Province Patentee before: LUDONG University |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220317 Granted publication date: 20180608 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |