CN106511915B - A kind of compound traditional Chinese medicine composite is inhibiting the application in cutaneous melanoma Lung metastases drug - Google Patents
A kind of compound traditional Chinese medicine composite is inhibiting the application in cutaneous melanoma Lung metastases drug Download PDFInfo
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Abstract
The invention discloses a kind of compound traditional Chinese medicine composites to inhibit the application in cutaneous melanoma Lung metastases drug, wherein the compound traditional Chinese medicine composite includes the component of following parts by weight: 15~30 parts of astragalus root, fry 15~30 parts of Radix Codonopsis, 10~20 parts of rhizoma atractylodis macrocephalae, 15~30 parts of Poria cocos, 15~30 parts of Chinese yam, 5~10 parts of radix glycyrrhizae preparata, 10~20 parts of dark plum, 10~20 parts of trigone, 10~20 parts of curcuma zedoary of toast, 15~30 parts of hairyvein agrimony, 15~30 parts of field pennycress.Compound composite medicament of the invention has in the case where not reducing immunity of organisms, significantly inhibits cutaneous melanoma Lung metastases, while the production of the medicine is cheap, application easy to spread.
Description
Technical field
The present invention relates to traditional Chinese medicine technologies, and in particular to a kind of compound traditional Chinese medicine composite is inhibiting cutaneous melanoma Lung metastases
Application in drug.
Background technique
Melanoma is the most important tumor type of dermatology, originates from the melanocyte at epidermal-dermal junction,
It can also be from positions such as mucous membrane or ocular choroids.This kind of tumour is the highest one kind of relatively rare but grade malignancy, in institute
Its disease incidence is about 0.1/10 ten thousand people in You Fei developed country, but its lethality accounts for 97%, is easier to DISTANT METASTASES IN occur, especially
Lung metastases, therefore should attract great attention.
As the overall incidence of compatriots' living-pattern preservation, the pollution of environment, tumour increases year by year.Tumor patient mesh
It is preceding in addition to operation other than, many First-line chemotherapies are still the Cocktail treatment based on cytotoxic drug.Chemotherapeutic
Although also having certain lethal effect for intracorporal normal cell using Partial tumors cell has been eliminated.Clinically patient
The case where using will appear hypoimmunity after chemotherapeutic, zoopery show the experiment mice after 5 FU 5 fluorouracil intervention,
Weight, spleen index, T lymphocyte proliferation activity, CD4/CD8 ratio are substantially reduced, oligoleukocythemia, atrophy of immune organ, huge
Phagocyte phagocytic function reduces and the decline of NK cell activity.
The patient of melanoma is sought to reduce its DISTANT METASTASES IN how on the basis of not lowering its immunity
The treatment method of supplement and substitution is just at the task of top priority, and traditional Chinese medicine treatment shows its unique advantage in this respect.Mesh
Before have a large amount of clinical and Basic Experiment Study show traditional Chinese medicine in terms of treat malignant tumour with good curative effect, in addition to
Outside the effect for directly killing tumour, traditional Chinese medicine can also play indirect effect, it being reached by strengthening vital QI to eliminate pathogenic factors improves disease
Shape is improved the quality of living, and is reduced transfer and is extended the purpose of life cycle.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of compound traditional Chinese medicine composites to inhibit cutaneous melanoma lung
Application in diversion medicaments.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of compound traditional Chinese medicine composite is inhibiting the application in cutaneous melanoma Lung metastases drug,
Wherein, the compound traditional Chinese medicine composite includes the component of following parts by weight: 15~30 parts of astragalus root, frying Radix Codonopsis
15~30 parts, 10~20 parts of rhizoma atractylodis macrocephalae, 15~30 parts of Poria cocos, 15~30 parts of Chinese yam, 5~10 parts of radix glycyrrhizae preparata, process dark plum 10~20
Part, 10~20 parts of trigone, 10~20 parts of curcuma zedoary, 15~30 parts of hairyvein agrimony, 15~30 parts of field pennycress.
Wherein, the compound traditional Chinese medicine composite preferably includes the component of following parts by weight:
15~20 parts of astragalus root, 15~20 parts of Radix Codonopsis of stir-fry, 10~15 parts of rhizoma atractylodis macrocephalae, 15~20 parts of Poria cocos, Chinese yam 15~30
Part, 5~10 parts of radix glycyrrhizae preparata, 10~15 parts of dark plum of toast, 10~15 parts of trigone, 10~15 parts of curcuma zedoary, 20~30 parts of hairyvein agrimony, Patrinia scaniosaefolia
20~30 parts of grass.
Wherein, the compound traditional Chinese medicine composite, dosage form are decoction, syrup, mixture, granule, tablet or capsule
Agent.
Wherein, the compound traditional Chinese medicine composite is prepared as follows to obtain: by the Chinese medicine astragalus root of formula ratio, frying
Radix Codonopsis, rhizoma atractylodis macrocephalae, Poria cocos, Chinese yam, radix glycyrrhizae preparata, toast dark plum, trigone, curcuma zedoary, hairyvein agrimony and field pennycress add water to cook to obtain the final product.
Wherein, it decocts every time, the weight of the water of addition is 5~20 times of Chinese medicine total weight；The number of decoction is 1~3
Secondary, each decocting time is 1~3 hour.
Decocting liquid is concentrated after can also adding water to cook for the preparation method of above-mentioned compound traditional Chinese medicine composite to obtain the final product.
Wherein, the concentration, method one are as follows: decocting liquid is concentrated into 60 DEG C of relative densities 1.05~1.15.
Wherein, the concentration, method two are as follows: decocting liquid is concentrated into 60 DEG C of relative densities 1.05~1.15, then plus ethyl alcohol
It is extracted to alcohol content up to 60~90%, after separation of solid and liquid, takes supernatant, recycle ethyl alcohol in supernatant to being concentrated to the greatest extent
Liquid.
The preparation method of above-mentioned compound medicine, is added or is added without sucrose into concentrate, sterilizing be made syrup or
Decoction；Or, dextrin, Steviosin are added into concentrate, tablet, capsule or granule is made.
Wherein, the compound traditional Chinese medicine composite is by inhibiting melanoma Lung metastases so as to improve the prognosis of disease.
Wherein, the compound traditional Chinese medicine composite can not lower immune cell number while reaching therapeutic effect
Amount.
Compound composite medicament provided by the present invention, the sweet temperature of astragalus root in side, arrogates to oneself air making-up and spleen enlivening, yang invigorating；Fry Radix Codonopsis
Gan Ping arrogates to oneself gas, the nourishing generate fluid of tonifying spleen and stomach, and two medicines share, and it is altogether monarch drug in a prescription that the function of QI invigorating tonifying spleen, which writes,.Rhizoma atractylodis macrocephalae air making-up and spleen enlivening is dry
The benefits of wet, Poria cocos invigorating spleen for diuresis excreting dampness, Chinese yam QI invigorating tonifying spleen yin-nourishing, three medicines associate with, dehumidifying fortune spleen is evident, and temper is filled, have dampness elimination it
Power, it is wet it is turbid go, increase the function of invigorating the spleen, be altogether ministerial drug, play the function of excreting dampness of replenishing qi to invigorate the spleen.Trigone, curcuma zedoary, bitter is violent, arrogates to oneself the heavily fortified point that disappears
Open the stasis of blood, with it in tonic, can both disappear hemostasis is slow, and borrow the power of its circulation, row tonic it is stagnant；The pungent scattered row of field pennycress is stagnant, arrogates to oneself
The carbuncle that disappears apocenosis, the blood-breaking row stasis of blood；Astringing to arrest bleeding, removing toxic substances stop dysentery are arrogated to oneself in the convergence of hairyvein agrimony puckery；It is sour and puckery to process dark plum, arrogates to oneself puckery intestines and stops
It rushes down, analgesic of promoting the production of body fluid, five medicines are adjutant row dissipating blood stasis with potent drugs, hold back the effect of yin removing toxic substances altogether.Radix glycyrrhizae preparata Gan Pingwei makes medicine, invigorate the spleen and benefit qi,
Clearing heat and detoxicating, coordinating the drug actions of a prescription.Therefore all medicines share, its righting tonifying Qi is taken, dissipating blood stasis with potent drugs holds back the effect of yin detoxifies.
According to an embodiment of the invention, using C57BL/6 mouse for experiment, can be lowered using this compound composite medicament black
The Lung metastases degree of melanoma model mice, meets the expection of Chinese medicine " strengthening vital QI to eliminate pathogenic factors ".
The utility model has the advantages that compared with prior art, compound composite medicament of the invention, which has, is not lowering immunity of organisms
In the case of, the curative effect of the significant Lung metastases degree for lowering melanoma, while the production of the medicine is cheap, it is easy to spread to answer
With.
Detailed description of the invention
Fig. 1: the Lung metastases situation after melanoma tumor model mouse Chinese medicine intervention compares.
Upper row: the lung of negative control group mouse；Lower row: the lung of Chinese medicine compound prescription group mouse.Black is to be transferred to lung in figure
Melanoma, white are lung tissue.It can be seen that the lung of control group almost all black, and the lung of Chinese medicine group still has white to deposit
?.
Specific embodiment
The present invention is described further combined with specific embodiments below.It, can be more preferable geographical according to following embodiments
The solution present invention.However, as it will be easily appreciated by one skilled in the art that content described in embodiment is merely to illustrate the present invention,
Without the present invention described in detail in claims should will not be limited.
Embodiment 1
A kind of compound composite medicament lowering melanoma Lung metastases, is grouped as by each group of following parts by weight:
15 parts of astragalus root, 15 parts of Radix Codonopsis are fried, 10 parts of rhizoma atractylodis macrocephalae, 15 parts of Poria cocos, 15 parts of Chinese yam, 5 parts of radix glycyrrhizae preparata, processs dark plum 10
Part, 10 parts of trigone, 10 parts of curcuma zedoary, 30 parts of hairyvein agrimony, 30 parts of field pennycress, totally 11 taste medicine.
In prescription ratio, the above 11 taste prepared slices of Chinese crude drugs are weighed, add the water of 10 times of bulk pharmaceutical chemicals total weight amounts, decocts and extracts 3 times,
1 hour every time.Collecting decoction, as decoction.
Resulting decoction is concentrated into relative density 1.10 (60 DEG C of surveys), adds ethyl alcohol to alcohol content up to 60%, stands 12 hours
More than.Leaching supernatant, recycling ethyl alcohol are concentrated into relative density about 1.10 (60 DEG C survey) to the greatest extent.Simple syrup is added, and adds and steams
Distilled water to the suitable medicine materical crude slice 1g of every mL, filtering, sterilizes to get compound medicine syrup at packing.
Embodiment 2
A kind of compound composite medicament lowering melanoma Lung metastases, is grouped as by each group of following parts by weight:
15 parts of astragalus root, 30 parts of Radix Codonopsis are fried, 10 parts of rhizoma atractylodis macrocephalae, 20 parts of Poria cocos, 15 parts of Chinese yam, 5 parts of radix glycyrrhizae preparata, processs dark plum 10
Part, 15 parts of trigone, 15 parts of curcuma zedoary, 30 parts of hairyvein agrimony, 30 parts of field pennycress, totally 11 taste medicine.
In prescription ratio, the above 11 taste prepared slices of Chinese crude drugs are weighed, add the water of 20 times of bulk pharmaceutical chemicals total weight amounts, decocts and extracts 3 times,
1 hour every time.Collecting decoction is concentrated into relative density 1.08 (60 DEG C of surveys), adds ethyl alcohol to alcohol content up to 60%, stand 24 hours
More than, leaching supernatant, recycling ethyl alcohol is concentrated into relative density about 1.08 (60 DEG C survey) to the greatest extent.Add distilled water to every mL phase
It as medicine materical crude slice 1g, mixes, filters, packing sterilizes to get compound medicine mixture.
Embodiment 3
A kind of compound composite medicament lowering melanoma Lung metastases, is grouped as by each group of following parts by weight:
20 parts of astragalus root, 20 parts of Radix Codonopsis are fried, 15 parts of rhizoma atractylodis macrocephalae, 15 parts of Poria cocos, 15 parts of Chinese yam, 10 parts of radix glycyrrhizae preparata, processs dark plum
15 parts, 10 parts of trigone, 10 parts of curcuma zedoary, 15 parts of hairyvein agrimony, 15 parts of field pennycress, totally 11 taste medicine.
In prescription ratio, the above 11 taste prepared slices of Chinese crude drugs are weighed, add the water of 5 times of bulk pharmaceutical chemicals total weight amounts, decocts and extracts 3 times,
1 hour every time.Collecting decoction is concentrated into relative density 1.10 (60 DEG C of surveys), adds ethyl alcohol to alcohol content up to 60%, stand 24 hours
More than, leaching supernatant, recycling ethyl alcohol is concentrated into relative density about 1.33 (60 DEG C survey) to the greatest extent.Appropriate dextrin, stevia rebaudianum is added
Glycosides is pelletized, dry, and whole grain dispenses to get compound medicine granule.
Embodiment 4
A kind of compound composite medicament lowering melanoma Lung metastases, is grouped as by each group of following parts by weight:
15 parts of astragalus root, 15 parts of Radix Codonopsis are fried, 10 parts of rhizoma atractylodis macrocephalae, 15 parts of Poria cocos, 15 parts of Chinese yam, 5 parts of radix glycyrrhizae preparata, processs dark plum 10
Part, 10 parts of trigone, 10 parts of curcuma zedoary, 20 parts of hairyvein agrimony, 20 parts of field pennycress, totally 11 taste medicine.
In prescription ratio, the above 11 taste prepared slices of Chinese crude drugs are weighed, add the water of 10 times of bulk pharmaceutical chemicals total weight amounts, decocts and extracts 3 times,
1 hour every time.Collecting decoction is concentrated into relative density 1.09 (60 DEG C of surveys), adds ethyl alcohol to alcohol content up to 60%, stand 24 hours
More than, leaching supernatant, recycling ethyl alcohol is concentrated into relative density about 1.35 (60 DEG C survey) to the greatest extent.Appropriate amount of starch, system is added
Grain, dry, whole grain, tabletting, coating dispense to get compound medicine tablet.
Embodiment 5
A kind of compound composite medicament lowering melanoma Lung metastases, is grouped as by each group of following parts by weight:
15 parts of astragalus root, 15 parts of Radix Codonopsis are fried, 10 parts of rhizoma atractylodis macrocephalae, 15 parts of Poria cocos, 15 parts of Chinese yam, 5 parts of radix glycyrrhizae preparata, processs dark plum 10
Part, 10 parts of trigone, 10 parts of curcuma zedoary, 30 parts of hairyvein agrimony, 30 parts of field pennycress, totally 11 taste medicine.
In prescription ratio, the above 11 taste prepared slices of Chinese crude drugs are weighed, add the water of 10 times of bulk pharmaceutical chemicals total weight amounts, decocts and extracts 2 times,
1 hour every time.Collecting decoction is concentrated into relative density 1.12 (60 DEG C of surveys), adds ethyl alcohol to alcohol content up to 60%, stand 24 hours
More than, leaching supernatant, recycling ethyl alcohol is concentrated into relative density about 1.32 (60 DEG C survey) to the greatest extent.Appropriate amount of starch, system is added
Grain, dry, whole grain is encapsulated, dispenses to get compound medicine capsule.
The experimental study of Chinese medicine compound prescription attenuating melanoma Lung metastases: the compound medicine of optional above-described embodiment 1-5 preparation
Prescription decoction is made, carry out the animal experiment of traditional Chinese compound medicine.
Embodiment 6
By taking typical 1 prescription of embodiment as an example, it is illustrated, other embodiments effect is suitable, specific as follows:
Experimental animal: SPF grades C57BL/6 mouse 16, half male and half female, 15~19g of weight must purchased from the western Poole-in Shanghai
Triumphant experimental animal Co., Ltd, quality certification number: SCXK (Shanghai) 2013-0016.
Experimental drug: the decoction of preparation 1 prescription of embodiment is concentrated into and is equivalent to crude drug concentration 6g/mL, this is compound medicine
Object.
Experimental animal grouping: 16 mouse are randomly divided into negative control group (physiological saline group) 8 and Chinese medicine compound prescription is controlled
Treatment group 8.Every equal tail vein injection 3*105A B16F10 mouse melanin tumor cell, the injection same day start groups of animals
Give corresponding pharmaceutical intervention.
Medication: negative control group gavages physiological saline 0.2mL/20g weight daily, and compound of Chinese herbal medicine group fills daily
Take traditional Chinese medicine compound (embodiment 1) 0.2mL/20g weight, it is above one time a day, continuous 24 days.2 hours after the last administration, take off neck
Vertebra puts to death animal, weighs, draws materials, and specific drug composition and administration mode are shown in Table 1.
1 drug of table composition and administration mode
Experimental result:
1) ordinary circumstance: situations such as observing mouse spirit, activity, diet, urine, excrement after administration daily does not find
Abnormal, eye, ear, nose, mouth are showed no secretion.After modeling, 16 mouse survive, but during experiment in 24 days, and two groups
Mouse is 3 dead, therefore survival rate is 67.5%, and Chinese medicine group is suitable with negative group.
2) weight of each group mouse and the change situation of spleen index, in treatment end, without obvious between two groups of mouse weight
Difference, Chinese medicine group spleen weight is larger, but the equal > 0.05 of p value, is specifically relatively shown in Table 2.
The case where when 2 each group mouse treatment end of table, compares (n=5)
| Group | Weight (g) | Spleen weight (g) | Spleen index |
| Negative control group (physiological saline) | 16.26±0.82 | 0.069±0.0195 | 0.004±0.0011 |
| Chinese medicine compound prescription group | 17.52±2.04 | 0.092±0.0237 | 0.005±0.0011 |
| P value (organizes ratio with feminine gender) | 0.253 | 0.137 | 0.209 |
3) influence of the Chinese medicine compound prescription to melanoma Lung metastases:
The comparison (n=5) of Pulmonary metastasis focuses size and quantity when 3 each group mouse treatment end of table
| Group | ≤1mm | 1~3mm | ≥3mm |
| Negative control group (physiological saline) | 9.0±5.6 | 14.4±5.4 | 14.4±3.8 |
| Chinese medicine compound prescription group | 15.6±3.6 | 7.8±4.3 | 3.8±2.0 |
| P value (organizes ratio with feminine gender) | 0.063 | 0.067 | 0.001 |
At the end of experiment, in the Pulmonary metastasis focuses of Chinese medicine intervention group, diameter≤1mm number is more than negative group, but diameter is 1
The transfer stove number of~3mm and >=3mm are that Chinese medicine group is less than negative group, especially >=3mm group, and p value has less than 0.05
Statistical significance prompts it to can be reduced the Lung metastases of melanoma.
4) influence of the Chinese medicine compound prescription to mouse immune cell content:
Influence of 4 Chinese herbs group of table to mouse peripheral blood immunocyte content
*: the content of macrophage is using all lymphocytes as denominator, with CD45 and F4/80 cell positive simultaneously
It is obtained for molecular computing.The content of #:NK cell be using all lymphocytes as denominator, it is positive simultaneously with CD45 and NK1.1
Cell obtains for molecular computing.
Influence of 5 Chinese herbs group of table to mouse spleen immunocyte content
*: the content of macrophage is using all lymphocytes as denominator, with CD45 and F4/80 cell positive simultaneously
It is obtained for molecular computing.The content of #:NK cell be using all lymphocytes as denominator, it is positive simultaneously with CD45 and NK1.1
Cell obtains for molecular computing.
At the end of experiment, we have detected the content of various immunocytes in the peripheral blood and spleen of mouse, compare Chinese medicine
Their change situation after intervention.The experimental results showed that (table 4 and table 5), either T cell, NK cell or macrophage,
It is not all decreased obviously (p < 0.05) after content medicine in use, illustrates the use of the Chinese medicine, the immunity to body is that do not have
The effect of weakening.
The experimental results showed that (Fig. 1), in animal integral experiment, the lung of negative control group almost all black is said
There is tumour cell in bright most lung tissue, and the lung of Chinese medicine compound prescription group animal still has the lung tissue of white.Pass through
Count the number of different size of transfer stove, it is seen that can be reduced Lung metastases after Chinese medicine intervention, illustrate that the herbal mixture is inhibiting black
Effect is obvious in terms of melanoma Lung metastases.
From the above experiment as can be seen that this Chinese medicine compound prescription can on the basis of not reducing all kinds of immunocyte contents of body,
It is played the effect of inhibiting in terms of melanoma Lung metastases, embodies the effect unique of the Chinese medicine compound prescription.
Claims (8)
1. a kind of compound traditional Chinese medicine composite inhibits the application in cutaneous melanoma Lung metastases drug in preparation,
Wherein, the compound traditional Chinese medicine composite is grouped as by the group of following parts by weight: 15~30 parts of astragalus root, frying Radix Codonopsis 15
~30 parts, 10~20 parts of rhizoma atractylodis macrocephalae, 15~30 parts of Poria cocos, 15~30 parts of Chinese yam, 5~10 parts of radix glycyrrhizae preparata, 10~20 parts of dark plum are processed,
10~20 parts of trigone, 10~20 parts of curcuma zedoary, 15~30 parts of hairyvein agrimony, 15~30 parts of field pennycress.
2. application according to claim 1, which is characterized in that the compound traditional Chinese medicine composite is by following parts by weight
Group is grouped as:
15~20 parts of astragalus root, 15~20 parts of Radix Codonopsis are fried, 10~15 parts of rhizoma atractylodis macrocephalae, 15~20 parts of Poria cocos, 15~30 parts of Chinese yam, is processed
5~10 parts of Radix Glycyrrhizae, 10~15 parts of dark plum of toast, 10~15 parts of trigone, 10~15 parts of curcuma zedoary, 20~30 parts of hairyvein agrimony, field pennycress 20
~30 parts.
3. application according to claim 1, which is characterized in that the compound traditional Chinese medicine composite, dosage form are decoction, sugar
Starch agent, mixture, granule, tablet or capsule.
4. application according to claim 1, which is characterized in that the compound traditional Chinese medicine composite is prepared as follows
It arrives: by the Chinese medicine astragalus root of formula ratio, frying Radix Codonopsis, rhizoma atractylodis macrocephalae, Poria cocos, Chinese yam, radix glycyrrhizae preparata, toast dark plum, trigone, curcuma zedoary, celestial being
Crane grass and field pennycress add water to cook to obtain the final product.
5. application according to claim 4, which is characterized in that decoct every time, the weight of the water of addition is Chinese medicine gross weight
5~20 times of amount；The number of decoction is 1~3 time, and each decocting time is 1~3 hour.
6. application according to claim 4 or 5, which is characterized in that after adding water to cook, decocting liquid is concentrated to obtain the final product.
7. application according to claim 6, which is characterized in that be added or be added without sucrose into concentrate, sterilizing is made
At syrup or decoction；Or, dextrin, Steviosin are added into concentrate, tablet, capsule or granule is made.
8. application according to claim 1 or claim 7, which is characterized in that the compound traditional Chinese medicine composite passes through strengthening vital QI to eliminate pathogenic factors
To reduce the Lung metastases degree of melanoma.
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| US20200397847A1 (en) * | 2019-06-18 | 2020-12-24 | St. Lotus Biotech Corp. | Herbal composition for inhibiting melanoma cell metastasis and manufacturing method thereof |
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