CN106509909A - Method of extracting amino acids from duck eggs - Google Patents
Method of extracting amino acids from duck eggs Download PDFInfo
- Publication number
- CN106509909A CN106509909A CN201610845290.9A CN201610845290A CN106509909A CN 106509909 A CN106509909 A CN 106509909A CN 201610845290 A CN201610845290 A CN 201610845290A CN 106509909 A CN106509909 A CN 106509909A
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- CN
- China
- Prior art keywords
- aminoacid
- anas domestica
- ovum anas
- duck eggs
- extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention discloses a method of extracting amino acids from duck eggs. The method comprises the four steps of material selecting, stirring, centrifuging and concentrating. The specific operation steps are as follows: step one, fresh and healthy duck eggs are selected and the selected duck eggs are hatched for two days to two weeks; step two, the duck eggs are de-shelled, the de-shelled duck eggs are stirred evenly, an ethanol aqueous solution at a concentration of 35-55% is added until the ethanol accounts for 25-35% of the total volume of a system, and the stirring continues until precipitation appears; step three, the obtained duck eggs added with the ethanol aqueous solution are subjected to a centrifugal treatment using a centrifuge, and a supernatant is taken; and step four, the obtained supernatant is subjected to a concentration treatment using a reverse osmosis membrane, and then a filtering membrane is used to conduct a filtration sterilization to obtain amino acid extracts. The method of extracting the amino acids from the duck eggs is simple in the technology, the used raw materials are safe and reliable, the reverse osmosis membrane and filtering membrane are used to conduct the concentration and filtration, the extraction precision is high, the use life is long, and the reverse osmosis membrane and filtering membrane can be washed, and facilitate a long-term use.
Description
Technical field
The invention belongs to membrance separation extractive technique field, and in particular to a kind of method for extracting aminoacid from Ovum Anas domestica.
Background technology
Aminoacid is the ultimate unit for constituting organism protein molecule, has close pass with biological vital movement
System, it has special physiological function in body, for the required raw material of synthetic hormone, enzyme and antibody, is that biology is internal not
One of nutritional labeling that can lack is viable organism nutrition, survive, develop particularly important material, and the thing in the life body
The aspects such as matter metabolic regulation, information transmission all play an important role.
Ovum Anas domestica contains abundant nutrient substance, and including protein, fat, aminoacid, minerals and vitamins etc., other are micro-
Amount nutritional labeling, in the middle of common eggs, the amino acid content highest of Ovum Anas domestica, with the development of fowl industry, Ovum Anas domestica yield is significantly
Improve, develop also relatively broad to which, extract aminoacid from Ovum Anas domestica, can solve the problems, such as that the fresh egg shelf-life is shorter.
The content of the invention
It is an object of the invention to provide the side for extracting aminoacid from Ovum Anas domestica of a kind of process is simple, purification high efficiency
Method.
The technical scheme is that a kind of method for extracting aminoacid from Ovum Anas domestica, including selection, stirring, centrifugation and concentration
Four steps, concrete operation step are as follows：
Step one：Fresh healthy Ovum Anas domestica is chosen, is hatched two days to two weeks；
Step 2：Shell, Ovum Anas domestica is stirred, add the ethanol water that concentration is 35-55%, system is accounted for up to ethanol total
The 25-35% of volume, continues stirring to appearance precipitation；
Step 3：Add to gained the Ovum Anas domestica of ethanol water centrifugal treating to be carried out with centrifuge, take supernatant；
Step 4：Supernatant to obtaining carries out concentration with reverse osmosis membrane, and reusing filter membrane carries out filtration sterilization process, obtains
To aminoacid extract.
Preferably, centrifugation described in step 3 is that 10-30 minutes are centrifuged under 6000-8000 rev/min of rotating speed.
Preferably, filter sizes described in step 4 are 0.45mm.
Preferably, aminoacid extract described in step 4 is applied in pharmacy, health product or food as raw material.
The present invention is a kind of method for extracting aminoacid from Ovum Anas domestica, by selection, stirring, four steps of centrifugation and concentration
It is withdrawn as the aminoacid extract of pharmacy, health product and raw-food material step by step from Ovum Anas domestica, chooses fresh and healthy
Ovum Anas domestica, through the hatching of two day time, in Ovum Anas domestica, contained protein, aminoacid, free amino acid all start gradually to increase,
Become the raw material of preferable aminoacid extract, but no more than two weeks, otherwise outward sense was formed, and is unfavorable for aminoacid
Separation and Extraction, add concentration be 35-55% ethanol water, stir to occur precipitation, then carried out with centrifuge from
The heart, takes supernatant, and retelling supernatant reverse osmosis membrane carries out concentration, finally carries out filtration sterilization with filter membrane, obtains amino
Sour extract.
Beneficial effects of the present invention：
The present invention be it is a kind of from Ovum Anas domestica extract aminoacid method, process is simple, the materials safety reliability for using, using reverse osmosis
Permeable membrane and filter membrane are concentrated and are filtered, and extraction accuracy is high, long service life, and reverse osmosis membrane and filter membrane can be cleaned, convenient to grow
Phase uses.
Description of the drawings
Fig. 1 is a kind of process chart of the method for extracting aminoacid from Ovum Anas domestica of the present invention.
Specific embodiment
For ease of skilled artisan understands that the present invention program, makees to technical solution of the present invention in conjunction with Figure of description
It is further elaborated with.
As shown in figure 1, the present invention be it is a kind of from Ovum Anas domestica extract aminoacid method, including selection, stirring,
Four steps of centrifugation and concentration, concrete operation step are as follows：
Step one：Fresh healthy Ovum Anas domestica is chosen, is hatched two days to two weeks；
Step 2：Shell, Ovum Anas domestica is stirred, add the ethanol water that concentration is 35-55%, system is accounted for up to ethanol total
The 25-35% of volume, continues stirring to appearance precipitation；
Step 3：Add to gained the Ovum Anas domestica of ethanol water centrifugal treating to be carried out with centrifuge, take supernatant；
Step 4：Supernatant to obtaining carries out concentration with reverse osmosis membrane, and reusing filter membrane carries out filtration sterilization process, obtains
To aminoacid extract.
Centrifugation described in step 3 is that 10-30 minutes are centrifuged under 6000-8000 rev/min of rotating speed.Described in step 4
Filter sizes are 0.45mm.Aminoacid extract described in step 4 is applied in pharmacy, health product or food as raw material.
The present invention is a kind of method for extracting aminoacid from Ovum Anas domestica, and the Ovum Anas domestica for choosing fresh and healthy hatches two days to two
Week, this period Ovum Anas domestica protein, aminoacid and free amino acid gradually can increase, smash Ovum Anas domestica, add toward Ovum Anas domestica dense
Spend for 35-50% ethanol waters, until 25-35% of the ethanol in system cumulative volume, continue stirring to there is precipitation, in centrifuging and taking
Liquid, supernatant is concentrated using reverse osmosis membrane, is reused filter membrane and is carried out filtration sterilization and obtains aminoacid extract.
The present invention program is exemplarily described to invention above in conjunction with accompanying drawing, it is clear that the present invention is implemented not
Limited by aforesaid way, as long as employing the various unsubstantialities improvement that method of the present invention design and technical scheme are carried out,
Or it is not improved the design of invention and technical scheme are directly applied to into other occasions, protection scope of the present invention it
It is interior.
Claims (4)
1. it is a kind of from Ovum Anas domestica extract aminoacid method, it is characterised in that including selection, stirring, centrifugation and concentrate four walk
Suddenly, concrete operation step is as follows：
Step one：Fresh healthy Ovum Anas domestica is chosen, is hatched two days to two weeks；
Step 2：Shell, Ovum Anas domestica is stirred, add the ethanol water that concentration is 35-55%, system is accounted for up to ethanol total
The 25-35% of volume, continues stirring to appearance precipitation；
Step 3：Add to gained the Ovum Anas domestica of ethanol water centrifugal treating to be carried out with centrifuge, take supernatant；
Step 4：Supernatant to obtaining carries out concentration with reverse osmosis membrane, and reusing filter membrane carries out filtration sterilization process, obtains
To aminoacid extract.
2. it is according to claim 1 it is a kind of from Ovum Anas domestica extract aminoacid method, it is characterised in that described in step 3
Centrifugation is that 10-30 minutes are centrifuged under 6000-8000 rev/min of rotating speed.
3. it is according to claim 1 it is a kind of from Ovum Anas domestica extract aminoacid method, it is characterised in that described in step 4
Filter sizes are 0.45mm.
4. it is according to claim 1 it is a kind of from Ovum Anas domestica extract aminoacid method, it is characterised in that described in step 4
Aminoacid extract is applied in pharmacy, health product or food as raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845290.9A CN106509909A (en) | 2016-09-24 | 2016-09-24 | Method of extracting amino acids from duck eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610845290.9A CN106509909A (en) | 2016-09-24 | 2016-09-24 | Method of extracting amino acids from duck eggs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106509909A true CN106509909A (en) | 2017-03-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610845290.9A Pending CN106509909A (en) | 2016-09-24 | 2016-09-24 | Method of extracting amino acids from duck eggs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259583A (en) * | 2017-08-20 | 2017-10-20 | 合肥信达膜科技有限公司 | A kind of method that film extracts amino acid in duck's egg |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586894A (en) * | 2015-01-15 | 2015-05-06 | 杨玉峰 | Method for extracting amino acids from eggs |
-
2016
- 2016-09-24 CN CN201610845290.9A patent/CN106509909A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586894A (en) * | 2015-01-15 | 2015-05-06 | 杨玉峰 | Method for extracting amino acids from eggs |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259583A (en) * | 2017-08-20 | 2017-10-20 | 合肥信达膜科技有限公司 | A kind of method that film extracts amino acid in duck's egg |
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| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170322 |
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| RJ01 | Rejection of invention patent application after publication |