CN106497815A - A kind of garden bulkholderia cepasea, the microbial inoculum containing the bacterium and its application and the method for passivation chromium - Google Patents
A kind of garden bulkholderia cepasea, the microbial inoculum containing the bacterium and its application and the method for passivation chromium Download PDFInfo
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Abstract
The present invention relates to the technical field of biological remediation of heavy metal chromium pollution, discloses a kind of garden bulkholderia cepasea, the microbial inoculum containing the bacterium and its application and a kind of method of passivation chromium, wherein, the preserving number of the garden bulkholderia cepasea is CGMCC NO：10843.The method of the passivation chromium of the present invention includes：It is CGMCC NO by preserving number：10843 garden bulkholderia cepasea and/or containing preserving number be CGMCC NO：The microbial inoculum of 10843 garden bulkholderia cepasea is contacted with pollution of chromium sample, to be passivated to the chromium in pollution of chromium sample.The garden bulkholderia cepasea of the present invention has higher chromium passivating ability, can tolerate the pollution of chromium of high concentration, easy to operate, can the soil of pollution of chromium or chromate waste water biological prosthetic during play a significant role.
Description
Technical field
A kind of the present invention relates to technical field of biological remediation of heavy metal chromium pollution, in particular it relates to garden
Bulkholderia cepasea, the microbial inoculum containing the bacterium and its application and a kind of method of passivation chromium.
Background technology
Chromium (Cr) and its change platform thing are the rows such as metallurgy, intermetallic composite coating, plating, process hides, paint, pigment
The conventional base stock of industry, produces a large amount of chromium-containing waste gas, waste water in the production process of above-mentioned industry and gives up
Slag, causes serious problem of environmental pollution.
The form of common chromium has trivalent chromium and Cr VI in the environment.Trivalent chromium is in soil often with indissoluble
In the form of hydroxide.Cr in solution3+Concentration very little, so toxicity very little；Cr VI dissolves
Degree is big, thus toxicity is very strong.Micro chromium is needed by human, but the intake chromium of excess will cause seriously
Consequence.Cr VI and trivalent chromium have carcinogenic teratogenesis.For many years, countries in the world are generally dirty by chromium
Dye is classified as High risk population.
As other heavy metal pollutions, the governance way of pollution of chromium mainly has two kinds：One is to change chromium to exist
Existing forms in soil, by hexavalent chrome reduction be trivalent chromium, reduce its transfer ability in the environment and
Bioavailability；Two is to remove chromium from contaminated soil.According to both thinkings, current soil
Earth recovery technique is mainly physics, chemical method, but big to soil disturbance, may cause secondary pollution
Problem, cost of investment are big, and these limitation constrain the large-scale use of technology.And biological restoration is not
Destruction plant growth needed for soil environment, secondary pollution will not be produced, can original place process, simple to operate.
Specifically, immobilization and stabilisation are will to be mixed (also may be used by the soil of pollution of chromium with certain adhesive
To be aided with certain reducing agent, for reduction of hexavalent chromium), chromium therein is fixed by adhesive, chromium is made
No longer migrate to surrounding environment.In numerous adhesives, cement and tripoli be considered as a kind of effectively,
It is easy to get and inexpensive product.Chromium-polluted soil is repaired using the method, soil need to be excavated, cost
Higher, treatment effect also needs further to be improved.
Chemical reduction method is a kind of in-situ remediation method, using iron filings, ferrous sulfate or some other easy
The chemical reducing agent (certain adhesive can also be aided with) for obtaining by hexavalent chrome reduction be trivalent chromium, shape
Into the compound of indissoluble, so as to reduce chromium animal migration in the environment and bioavailability, and then mitigate
The harm of pollution of chromium.But in processing procedure, reducing agent is possible to be flushed away, it is also possible to by other materials
Oxidation.In addition, the reducing agent added in soil is likely to cause secondary pollution.Therefore.Soil particle
Internal chromic removal is the difficult point of chemical reduction method.
Chemical cleaning method be using hydraulic head promote cleaning fluid by contaminated soil by chromium from soil
Clear out, then again the cleaning fluid containing chromium is processed.Cleaning fluid may contain certain complexing agent,
Or clear water.The cleaning agent that same chemical cleaning method is introduced is likely to cause secondary pollution, and only fits
The big soil of suitable infiltration coefficient.
Biological restoration refers to appliable plant and microorganism to administer pollution of chromium.The improvement of pollution of chromium now
In terms of focusing primarily upon microorganism, i.e., using native soil in indigenous microorganism or to pollution environment supplement warp
The high-effective microorganism of domestication is crossed, under the operating condition for optimizing, by bioreduction, by Cr VI
Trivalent chromium is reduced to, so as to repair contaminated soil.The method will not produce secondary pollution, therefore screen
Going out plant height effect chromium passivating bacterial strain, to be applied to actual soil remediation most important.
Content of the invention
The invention aims to overcome pollution of chromium biological prosthetic present in drawbacks described above, there is provided a kind of
Garden bulkholderia cepasea, the microbial inoculum containing the bacterium and its application and a kind of method of passivation chromium.This
Bright garden bulkholderia cepasea, with higher chromium passivating ability, can tolerate the pollution of chromium of high concentration,
Easy to operate, can the soil of pollution of chromium or chromate waste water biological prosthetic during play a significant role.
To achieve these goals, the present inventor has carried out substantial amounts of screening experiment, as a result screens
To a kind of garden Burkholderia with higher chromium passivating ability, the pollution of chromium of tolerable high concentration
Bacterium.Therefore, in a first aspect, a kind of the invention provides garden bulkholderia cepasea (Burkholderia
Anthina), the preserving number of the garden bulkholderia cepasea is CGMCC NO：10843.
Second aspect, the invention provides a kind of contain above-mentioned garden bulkholderia cepasea
The microbial inoculum of (Burkholderia anthina).
The third aspect, the invention provides above-mentioned garden bulkholderia cepasea and/or microbial inoculum are in passivation chromium
In application.
Fourth aspect, the invention provides a kind of method of passivation chromium, methods described includes：By above-mentioned flower
Garden bulkholderia cepasea and/or above-mentioned microbial inoculum are contacted with pollution of chromium sample, with to the chromium in pollution of chromium sample
It is passivated.
The garden bulkholderia cepasea of the present invention is heavy metal chromium efficient passivation bacterial strain, can reduce chromium and exist
Bioavailability in water phase or soil phase.Compared with prior art, primary gram of garden Hall of the invention
Moral Salmonella can be in efficient passivation soil phase (little to soil disturbance) or water phase chromium, can tolerate high concentration
Pollution of chromium, easy to operate, can the soil of pollution of chromium or chromate waste water biological prosthetic during play
Important function, realizes the purpose that environment-friendly and green is repaired.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Biological deposits
The garden bulkholderia cepasea (Burkholderia anthina) of the present invention, in May, 2015
It is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address within 22nd：Beijing
The institute 3 of city Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode：100101)
(depositary institution is abbreviated as CGMCC), deposit number is CGMCC：10843.
Specific embodiment
Hereinafter the specific embodiment of the present invention is described in detail.It should be appreciated that this place is retouched
The specific embodiment that states is merely to illustrate and explains the present invention, is not limited to the present invention.
In a first aspect, a kind of the invention provides garden bulkholderia cepasea (Burkholderia
Anthina), the preserving number of the garden bulkholderia cepasea is CGMCC NO：10843.
The preserving number of the present invention is CGMCC NO：10843 garden bulkholderia cepasea, is leather
Lan Shi negative bacteriums, belong to Burkholderia section Burkholder and belong to garden bulkholderia cepasea.The bacterial strain comes
Xuancheng Profile, anhui Province chromium-polluted soil is come from, and dilution plate method is screened and is passed through using soil circulating current system and is separated
Purifying is obtained.Biological characteristics is：Bending rod-short, without gemma, white circular protrusions；Gram contaminates
Color experiment, product H2S experiments, amylase experiment are negative, gelatin liquefaction test, oxidase test, contact
Enzyme test, nitrate reduction test are positive, using mannose.
Wherein, the separation process of garden bulkholderia cepasea of the invention can include：By 100g soil
The sand grains that earth is about 3mm with appropriate particle diameter is well mixed, and is placed in the upper strata of circulation enriching apparatus, 200ml
LB culture mediums are placed in lower floor as circulation liquid.Start peristaltic pump, according to the steaming of circulation liquid in enrichment process
Heat condition periodically adds circulation liquid.After enrichment terminates, upper layer of soil and lower floor's circulation liquid is taken, and by supernatant
Liquid dilutes 10 respectively-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8, draw and coat in right amount
Containing 50,100,200,300mg/L Cr6+LB solid mediums on, 25-30 DEG C culture 3 days after
Taking bacterial strain carries out plate streaking, separates single bacterium colony.
The present invention obtains one plant of most strong bacterial strain of heavy metal chromium resistance from the bacterial strain for filtering out
W-10, carries out Physiology and biochemistry identification and analysis to the bacterial strain, and its biological characteristics is：Bending rod-short, nothing
Gemma, white circular protrusions；Gram staining experiment, product H2S experiments, amylase experiment are negative, bright
Dispergation test, oxidase test, catalase test, nitrate reduction test are positive, using sweet dew
Sugar.Meanwhile, according to Tiangeng bacterial genomes DNA extraction kit (TIANamp bacteria DNA kit)
Step extracts the DNA of the bacterial strain, carries out 16S rDNA gene sequencings, its 16s rDNA gene
As a result sequence shows that the bacterium is garden bulkholderia cepasea as shown in SEQ ID NO.1
(Burkholderia anthina).The garden bulkholderia cepasea is preserved in Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, preserving number is CGMCC NO：10843.
The garden bulkholderia cepasea that the present invention is provided can produce a large amount of garden Bai Kehuo through culture
The viable bacteria body of your moral Salmonella, the method for the culture is not particularly required, as long as the garden can be made
Bulkholderia cepasea is bred, for example, it is possible to according to 107The inoculum concentration of CFU/mL is by garden primary
The viable bacteria body of Ke Huoerde Salmonellas is inoculated in LB culture mediums, at a temperature of 25-38 DEG C cultivates 12-36
After hour, nutrient solution is obtained.
In the present invention, for the condition of culture of garden bulkholderia cepasea, there is no particular limitation, can be with
For various condition of culture commonly used in the art, for example, the composition of LB fluid nutrient mediums used during culture
Can be：0.8-1 weight % peptones, 0.5-0.8 weight % dusty yeasts, 1-1.5 weight % sodium chloride,
PH=6.8-7.0.Agar in solid LB media also containing 2.5-3.0 weight %.Used during culture
The composition of minimal medium can be：0.8-1.2 weight %KH2PO4, 0.8-1.2 weight %K2HPO4,
1-1.5 weight %NH4NO3, 0.03-0.08 weight %MgSO4, 0.001-0.003 weight %CaCl2,
0.01-0.03 weight %FeSO4·7H2O, pH=6.0-7.5.
The present invention can further separate the viable bacteria body of the garden bulkholderia cepasea in above-mentioned nutrient solution,
The detached method has no particular limits, as long as thalline can be enriched with from nutrient solution, for example
The method that centrifugation can be passed through and/or filtered realizes that the condition of the centrifugation and the filtration can be known
Condition, the present invention will not be described here.
The present invention further can also obtain intracellular carrying from the viable bacteria body of garden bulkholderia cepasea
Thing is taken, there is no particular limitation for the method for obtaining intracellular extract, can be commonly used in the art
Various methods, for example, can be to carry out ultrasonication, centrifuging and taking supernatant under condition of ice bath to thalline.
Second aspect, the invention provides be CGMCC NO containing preserving number：10843 garden primary
The microbial inoculum of Ke Huoerde Salmonellas (Burkholderia anthina).
The present inventor is found surprisingly that in the course of the study, the garden Burkholder of the present invention
The viable bacteria body and dead thalline of Salmonella effectively heavy metal chromium can be passivated.Therefore, as above
The thalline of garden bulkholderia cepasea can be viable bacteria body, or dead thalline, can also be viable bacteria
The mixing thalline of body and dead thalline, i.e. microbial inoculum contain the garden bulkholderia cepasea dead thalline and/
Or viable bacteria body.But more it is surprising that the present inventor is it has furthermore been found that what the present invention was provided
The intracellular extract of garden bulkholderia cepasea, the passivation effect of heavy metal chromium more preferably, therefore,
Under preferable case, microbial inoculum contains the intracellular extract of the garden bulkholderia cepasea.
According to the present invention, for the preparation method of above dead thalline has no particular limits, for example but do not limit
In, can by naturally cracking prepare, it is also possible to prepared by Thermal killed, condition of ice bath can also be passed through
Prepared by lower ultrasonication, wherein prepare effect with ultrasonication under condition of ice bath optimum.For obtaining cell
There is no particular limitation for the method for interior extract, can be various methods commonly used in the art, for example can be with
It is to carry out ultrasonication, centrifuging and taking supernatant under condition of ice bath to thalline.
In the present invention, to the concentration in garden bulkholderia cepasea described in microbial inoculum or intracellular extract
There is no particular limitation for amount, specifically can be selected according to specific circumstances, and here is no longer gone to live in the household of one's in-laws on getting married in detail
State.
In addition, different according to predetermined purposes, the microbial inoculum that the present invention is provided can be prepared as different formulations,
And add the compositions such as corresponding excipient.Wherein, add which kind of excipient is in the microbial inoculum of which kind of formulation
Known to one of skill in the art, in this not go into detail.
The third aspect, the invention provides above-mentioned garden bulkholderia cepasea and/or microbial inoculum are in passivation chromium
In application.Under preferable case, soil or chromate waste water of the chromium source for pollution of chromium.
In the present invention, " passivation chromium " refers to the chromic content of heavy metal and its life in reduction pollution of chromium sample
Thing validity, so that reach the purpose of the environment of remediating heavy metal pollution of chromium.
Fourth aspect, the invention provides a kind of method of passivation chromium, the method includes：By preserving number it is
CGMCC NO：10843 garden bulkholderia cepasea and/or containing preserving number be CGMCC NO：
The microbial inoculum of 10843 garden bulkholderia cepasea is contacted with pollution of chromium sample, in pollution of chromium sample
Chromium be passivated.
In the inventive method, for the method for contacting, there is no particular limitation, can be commonly used in the art
Various methods, it is CGMCC NO that for example can add preserving number in pollution of chromium sample：10843
Garden bulkholderia cepasea and/or containing preserving number be CGMCC NO：Primary gram of 10843 garden
The microbial inoculum of Hall moral Salmonella, is well mixed.
In the method for the present invention, the present inventor has found under study for action, adds in pollution of chromium sample
The nutrient source of a certain amount of garden bulkholderia cepasea can effectively accelerate the passivation of chromium.It is therefore preferable that
In the case of, the method also includes：Nutrient source is added in pollution of chromium sample, it is further preferred that described
Nutrient source is nutrient broth nutrient solution, corn gluten meal peptone nutrient solution, yeast extract peptone nutrient solution, LB
At least one in nutrient solution and inorganic salts nutrient solution.
In the inventive method, under preferable case, the sample is soil or chromate waste water.
In the inventive method, for the form added to the garden bulkholderia cepasea in pollution of chromium sample
It is not particularly limited, as long as the garden bulkholderia cepasea can be in the chromium after ensureing to add
Work in contaminated samples and heavy metal chromium is effectively passivated, primary gram of the garden Hall of addition
The form of moral Salmonella, for example, it is possible to be thalline or the bacterium solution that cultivates to logarithmic phase, or freezing is dry
Thalline dry powder after dry, can also be its intracellular extract.
The present invention does not have special to the quantity of the garden bulkholderia cepasea for adding and the amount of microbial inoculum yet
Limit, this can determine according to the content of the chromium in the pollution of chromium sample and passivation complexity,
For example, when the chromium content in the sample is higher or more difficult passivation or for the garden Burkholderia
When the existence of bacterium is less favorable, the inoculum concentration of the garden bulkholderia cepasea and adding for microbial inoculum can be improved
Enter amount；When the chromium content in the sample is relatively low or is easier to passivation or to the garden bulkholderia cepasea
Existence impact less when, it is possible to reduce the inoculum concentration of the garden bulkholderia cepasea and microbial inoculum
Addition.
According to the present invention, when pollution of chromium sample is soil, in order to further promote the flower of present invention offer
Passivation efficiency of the garden bulkholderia cepasea to chromium, it is preferred that control the water content in soil at least
15 weight %, more preferably 18-30 weight %.
Embodiment
Hereinafter will be described the present invention by embodiment and comparative example, but and be not so limited this
Invention.
In experimental technique in following examples and comparative example, if no special instructions, this area routine is
Method.Experiment material used in following embodiments and comparative example, if no special instructions, is from routine
Biochemical reagents shop is commercially available.
Hexavalent chromium removal rate=(content of 6-valence Cr ions after before processing content of 6-valence Cr ions-process)/before processing Cr VI
Content × 100%.
Preparation example
It is CGMCC NO by the preserving number of the present invention：10843 garden bulkholderia cepasea is in LB
Activate 2 times in fluid nutrient medium, activation every time is carried out at 170rpm, 30 ± 1 DEG C 12 hours, is obtained
To bacterium solution, the bacterium solution for obtaining is inoculated in 300ml LB fluid nutrient mediums with the inoculum concentration of 3 volumes %,
Cultivated under 170rpm, 30 ± 1 DEG C of condition of culture, after 3h, entered logarithmic phase, reached after 9h steady
Periodically, bacteria concentration OD600About 7.
Embodiment 1
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：10843 garden Bai Kehuo
That tolerance and passivation ability of the moral Salmonella in water phase to chromium
Potassium bichromate is added in 300ml LB fluid nutrient mediums, Cr is made6+Concentration respectively 50mg/L,
100mg/L and 200mg/L, sterilize at 121 DEG C 15min.To in the aforementioned LB culture mediums after sterilizing
The bacterium solution that preparation example is obtained is accessed with the inoculum concentration of 3 volumes %.170rpm, cultivates at 30 ± 1 DEG C, sampling
Survey bacterium solution OD600Value and chromic concentration simultaneously calculate hexavalent chromium removal rate.Wherein, chromic concentration
It is measured with ICP-AES methods, assay method includes：Appropriate bacterium solution is taken, 8000rpm is centrifuged 5min,
Supernatant is taken, using Cr in ICP-AES detection supernatants6+Concentration, the condition of ICP-AES include：Survey
The a length of 267.7nm of standing wave.
As a result show：During 12h, in Cr6+Concentration be 50mg/L, 100mg/L and 200mg/L under,
Highest OD that the bacterial strain can reach600Value is respectively 4.33,6.23 and 7.37, to Cr6+Clearance point
Wei 45.33%, 55.60% and 83.9%.Show that preserving number of the invention is CGMCC NO：10843
Garden bulkholderia cepasea can not only enduring high-concentration pollution of chromium, and also have higher chromium blunt
Change ability.
Embodiment 2
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：Primary gram of 10843 garden
Passivation effect of the Hall moral Salmonella under the conditions of oligotrophic to Chromium in Soil
100ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, then access 1 volume %
The bacterium solution that preparation example is obtained, 170rpm, cultivates 12h at 30 ± 1 DEG C.By bacterium solution under 8000rpm from
Heart 5min, the thalline for obtaining is washed with aseptic deionized water and the resuspended water for making 100ml bacterium
Solution, the aqueous solution of the bacterium is added to the contaminated soil of 1kg heavy metal chromiums then, and (content of 6-valence Cr ions is
In 52.36mg/kg), appropriate aseptic deionized water is added, is stirred, cultivate 20d, it is ensured that per
The water content of its soil is 20%.After 20 day, soil is dried at 70 DEG C, accurately 0.5g is weighed,
Add the HNO of 65 weight % of 10ml3Micro-wave digestion 20min at 195 DEG C, takes supernatant liquid filtering afterwards,
Cr is determined with ICP-AES6+Content.Cr in soil6+23.88mg/kg is dropped to by 52.36mg/kg.Table
In the bright chromium-polluted soil processed through this bacterial strain, the content of chromium is decreased obviously, and illustrates in oligotrophic condition
Under, the bacterial strain has good effect to the passivation of heavy metal chromium in soil, can effectively reduce chromium in soil
Bioavailability in earth.
Embodiment 3
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：Primary gram of 10843 garden
Passivation effect of the Hall moral Salmonella under the conditions of eutrophy to Chromium in Soil
100ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, then access the system of 1 volume %
The bacterium solution that standby example is obtained, 170rpm, cultivates 12h at 30 ± 1 DEG C.Aforementioned bacterium solution is added to 1kg weights
In the contaminated soil (content of 6-valence Cr ions is 52.36mg/kg) of crome metal, appropriate aseptic deionized water is added,
Stir, cultivate 20d, it is ensured that the water content of soil is 20% daily.After 20 days, soil is existed
Dry at 70 DEG C, accurately weigh 0.5g, add the HNO of 65 weight % of 10ml3Microwave at 195 DEG C afterwards
20min is cleared up, supernatant liquid filtering is taken, and Cr is determined with ICP-AES6+Content.Cr in soil6+By
52.36mg/kg drop to 8.38mg/kg.Show the content through chromium in the chromium-polluted soil that this bacterial strain was processed
It is decreased obviously, illustrates under the conditions of eutrophy, the bacterial strain has to the passivation of heavy metal chromium in soil good
Effect, and be substantially better than oligotrophic condition, can more effectively reduce bioavailable of the chromium in soil
Degree.
Embodiment 4
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：Primary gram of 10843 garden
Hall moral Salmonella in water phase different shape to chromic passivation ability
100ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, then access the system of 1 volume %
The bacterium solution that standby example is obtained, 170rpm, cultivates 12h at 30 ± 1 DEG C.By 4 DEG C of centrifuging and taking supernatants of bacterium solution, i.e.,
For Supernatant samples.The aforementioned thalline 2 for obtaining is washed with 10mmol/L Tris-HCI buffer solutions (pH 7.0)
Resuspended after secondary, it is divided into 3 parts.Portion does not deal with, as resting cell sample；A addition 1
Volume % toluene and 0.2 volume %Triton X-100 vibrations, as permeabilized cells sample；Portion is in ice
Ultrasonication under the conditions of bath, supersonic frequency are 25KHz, and ultrasonic time is 20min, in 4 DEG C of centrifuging and takings
Clearly, as intracellular extract sample.Four parts of samples are separately added into a certain amount of potassium bichromate, make Cr6+
Concentration is 50mg/L.The Cr for being centrifuged after 12h and determining in supernatant6+Concentration, hexavalent chromium removal rate result
As shown in table 1.
Table 1
| Project | Hexavalent chromium removal rate |
| Supernatant samples | 25.8% |
| Resting cell sample | 57.6% |
| Permeabilized cells sample | 73.6% |
| Intracellular extract sample | 88.5% |
As can be seen from Table 1, intracellular extract sample is up to 88.5% to hexavalent chromium removal rate, remote high
In Supernatant samples, resting cell sample and permeabilized cells sample, show the CGMCC NO of the present invention：
The intracellular extract sample of 10843 garden bulkholderia cepasea can more effectively be passivated chromium.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited to above-mentioned reality
The detail in mode is applied, in the range of the technology design of the present invention, can be to the technical side of the present invention
Case carries out multiple simple variants, and these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique described in above-mentioned specific embodiment is special
Levy, in the case of reconcilable, can be combined by any suitable means, in order to avoid need not
The repetition that wants, the present invention are no longer separately illustrated to various possible combinations.
Additionally, can also be combined between a variety of embodiments of the present invention, as long as its
Without prejudice to the thought of the present invention, which should equally be considered as content disclosed in this invention.
Claims (10)
1. a kind of garden bulkholderia cepasea (Burkholderia anthina), it is characterised in that should
The preserving number of garden bulkholderia cepasea is CGMCC NO：10843.
2. a kind of microbial inoculum, it is characterised in that the microbial inoculum contains the garden Bai Kehuo described in claim 1
That moral Salmonella (Burkholderia anthina).
3. microbial inoculum according to claim 2, wherein, the microbial inoculum contains primary gram of garden Hall
The dead thalline and/or viable bacteria body of moral Salmonella.
4. microbial inoculum according to claim 2, wherein, the microbial inoculum contains primary gram of garden Hall
The intracellular extract of moral Salmonella.
5. any one in garden bulkholderia cepasea and/or claim 2-4 described in claim 1
Application of the microbial inoculum described in passivation chromium.
6. application according to claim 5, wherein, chromium source is the soil of pollution of chromium or useless containing chromium
Water.
7. a kind of passivation chromium method, it is characterised in that methods described includes：By claim 1 institute
The garden bulkholderia cepasea that states, and/or the microbial inoculum in claim 2-4 described in any one is dirty with chromium
Dye sample contact, to be passivated to the chromium in pollution of chromium sample.
8. method according to claim 7, wherein, methods described also includes：Dirty in the chromium
Nutrient source is added in dye sample.
9. method according to claim 8, wherein, the nutrient source be nutrient broth nutrient solution,
In corn gluten meal peptone nutrient solution, yeast extract peptone nutrient solution, LB nutrient solutions and inorganic salts nutrient solution
At least one.
10. the method according to any one in claim 7-9, wherein, the sample is soil
Or chromate waste water.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670980A (en) * | 2016-04-20 | 2016-06-15 | 国家烟草质量监督检验中心 | Microbial strain capable of remedying heavy metal-contaminated soil and application of microbial strain |
| CN107487868A (en) * | 2017-10-16 | 2017-12-19 | 中南大学 | A kind of method that nano cupric oxide cooperates with Cr VI in water removal with bacterium |
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