CN106497814B - A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for being passivated arsenic - Google Patents
A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for being passivated arsenic Download PDFInfo
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Abstract
The present invention relates to the technical field of biological remediation of heavy metal arsenic pollution, disclose a kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and a kind of method for being passivated arsenic, wherein the deposit number of the acinetobacter calcoaceticus sepsis bacterium is CGMCC NO:10855.The method of passivation arsenic of the invention includes: to contact acinetobacter calcoaceticus sepsis bacterium that deposit number is CGMCC NO:10855 and/or microbial inoculum containing the acinetobacter calcoaceticus sepsis bacterium that deposit number is CGMCC NO:10855 with arsenic pollution sample, to be passivated to the arsenic in arsenic pollution sample.Acinetobacter calcoaceticus sepsis bacterium arsenic passivation ability with higher of the invention can tolerate the arsenic pollution of high concentration, easy to operate, can play a significant role during the soil of arsenic pollution or the biological prosthetic of arsenic-containing waste water.
Description
Technical field
The present invention relates to the technical field of biological remediation of heavy metal arsenic pollution, and in particular, to a kind of acinetobacter calcoaceticus sepsis
Bacterium, the microbial inoculum containing the bacterium and its application and a kind of method for being passivated arsenic.
Background technique
Arsenic (As) is one of five big hypertoxic elements.Arsenic in soil is mainly derived from natural and artificial two big approach.Naturally
Source is mainly the contained arsenic element of soil itself, and in addition to rich arsenic area, most of arsenic in soil background value is about 15mg/
kg.Soil arsenic pollution is mainly derived from mankind's activity.Arsenic element and its derivative are widely used in manufacturing industry, as electronics produces
Product, alloy and coating etc..Metal mining simultaneously and coal mining activity can also discharge a large amount of arsenic into soil and atmospheric environment.
Pesticide containing arsenic and chemical fertilizer used in agricultural production are also a kind of important sources of arsenic in soil.Arsenide is once widely used in agriculture
Make insecticide, thimerosal, fungicide and herbicide in industry.In addition, also containing a certain amount of arsenic in some chemical fertilizer.It is general in phosphate fertilizer
Arsenic containing 20-50mg/kg, wherein the arsenic content in compound fertilizer is higher, and chronic administration makes the arsenic element of enriched in soil,
Cause seriously to pollute.Inorganic arsenic chemicals persistently exist in the soil, and can be utilized by plant, and then poisonous plant influences to make
Object growth.
In view of the characteristic of arsenic itself, it is necessary to manually be administered and be repaired.The method that can be used includes physical method, changes
Method and biological method, there is the following aspects: immobilization/stabilization technology, vitrification, Soil leaching technology,
Electric repairing technique in situ.Above-mentioned restorative procedure can destroy ecological environment of soil to some extent.That is, being adopted at present
Physics, chemical remediation technology are not only complicated for operation, but also used chemical reagent or physical operations can be to soil environments
Cause irreversible destruction.On the contrary, bioremediation technology repairing effect it is good, it is with low investment, be easy to implement, do not generate secondary pollution.
Therefore, in order to keep the activity of soil texture and microorganism, filtering out the arsenic in efficient passivation bacterial strain passivation soil is at present urgently
Problem to be solved.
Summary of the invention
The purpose of the invention is to overcome arsenic pollution it is biological prosthetic present in drawbacks described above, a kind of acinetobacter calcoaceticus is provided
Sepsis bacterium, the microbial inoculum containing the bacterium and its application and a kind of method for being passivated arsenic.Acinetobacter calcoaceticus sepsis bacterium of the invention, have compared with
High arsenic passivation ability can tolerate the arsenic pollution of high concentration, easy to operate, can be in the soil of arsenic pollution or the biology of arsenic-containing waste water
It plays a significant role in repair process.
To achieve the goals above, the present inventor has carried out a large amount of screening experiment, as a result screens a kind of tool
There is the acinetobacter calcoaceticus sepsis bacterium of higher arsenic passivation ability, the arsenic pollution that can tolerate high concentration.Therefore, in a first aspect, it is of the invention
A kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus) is provided, the deposit number of the acinetobacter calcoaceticus sepsis bacterium is
CGMCC NO:10855.
Second aspect, the present invention provides one kind to contain above-mentioned acinetobacter calcoaceticus sepsis bacterium (Acinetobacter
Septicus microbial inoculum).
The third aspect, the present invention provides the application of above-mentioned acinetobacter calcoaceticus sepsis bacterium and/or microbial inoculum in passivation arsenic.
Fourth aspect, the present invention provides a kind of methods for being passivated arsenic, which comprises by above-mentioned acinetobacter calcoaceticus sepsis
Bacterium and/or above-mentioned microbial inoculum are contacted with arsenic pollution sample, to be passivated to the arsenic in arsenic pollution sample.
Acinetobacter calcoaceticus sepsis bacterium of the invention is heavy metal arsenic efficient passivation bacterial strain, can reduce arsenic in water phase or soil phase
In bioavailability.Compared with prior art, acinetobacter calcoaceticus sepsis bacterium of the invention can efficient passivation soil phase (to soil
Earth disturbance is small) or water phase in arsenic, can tolerate the arsenic pollution of high concentration, it is easy to operate, can the soil of arsenic pollution or containing arsenic it is useless
The biological prosthetic of water plays a significant role in the process, realizes the purpose of environment-friendly and green reparation.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
Fig. 1 is the single colonie figure that plate streaking obtains.
Fig. 2 is AgNO3Solution covers the bacterium colony effect picture after acinetobacter calcoaceticus sepsis bacterium bacterium colony.
Biological deposits
Acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus) of the invention, was preserved on May 22nd, 2015
In China Committee for Culture Collection of Microorganisms's common micro-organisms center (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution is abbreviated as CGMCC), deposit number is
CGMCC:10855.
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
In a first aspect, this is not the present invention provides a kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus)
The deposit number of lever bacterium sepsis bacterium is CGMCC NO:10855.
Deposit number of the invention is the acinetobacter calcoaceticus sepsis bacterium of CGMCC NO:10855, is Gram-negative bacteria, belongs to not
Laplace Cordycepps acinetobacter sepsis bacterium.The bacterium source is screened in Xuancheng Profile, anhui Province As polluted soil using soil circulating current system
And it isolates and purifies to obtain by dilution plate method.Biological characteristics include: that morphological feature is the bacterium colony on LB solid medium
Smooth for faint yellow, round, neat in edge, surface wettability, micro- sem observation is rod-shaped in tubbiness, atrichia；Gram's staining examination
It tests, amylase experiment, oxidase test, nitrate reduction test, produce H2S experiment is negative, gelatin liquefaction test, catalase test
The positive cannot utilize mannose.
Wherein, the separation process of acinetobacter calcoaceticus sepsis bacterium of the invention may include: by 100g soil and appropriate partial size about
It is uniformly mixed for the sand grains of 3mm, is placed in the upper layer of circulation enriching apparatus, 200ml LB culture medium is placed in lower layer as circulation liquid.
Start peristaltic pump, circulation liquid is periodically added according to the evaporation situation of circulation liquid in enrichment process.After enrichment, upper layer of soil is taken
Plate streaking purifies and separates are carried out with lower layer's circulation liquid: supernatant is diluted 10 respectively-1、10-2、10-3、10-4、10-5、10-6、
10-7、10-8, draw and be coated in right amount containing 25,50,100,150,200,400mg/L As3+LB solid medium on, 25-
30 DEG C culture 3 days after take bacterium colony carry out plate streaking, separate single colonie, as shown in Figure 1.
The present invention obtains one plant of strains A s-T strongest to heavy metal arsenic resistance from the bacterial strain filtered out, to the bacterium
Strain carries out Physiology and biochemistry identification and analysis, and biological characteristics include: that morphological feature is that bacterium colony is yellowish on LB solid medium
Color, circle, neat in edge, surface wettability are smooth, and micro- sem observation is rod-shaped in tubbiness, atrichia；Gram stain test, starch
Enzyme experiment, nitrate reduction test, produces H at oxidase test2S experiment is negative, and gelatin liquefaction test, catalase test are positive, no
Mannose can be utilized.Meanwhile according to Tiangeng bacterial genomes DNA extraction kit (TIANamp bacteria DNA kit) step
Suddenly the DNA of the bacterial strain is extracted, 16S rDNA gene sequencing, 16s rDNA gene order such as SEQ ID NO.1 institute are carried out
Show, the results showed that the bacterium is acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus).By the acinetobacter calcoaceticus sepsis bacterium bacterium
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO:10855.
Acinetobacter calcoaceticus sepsis bacterium provided by the invention can generate the viable bacteria body of a large amount of acinetobacter calcoaceticus sepsis bacterium by culture,
The method of the culture does not require particularly, as long as the acinetobacter calcoaceticus sepsis bacterium can be made to be proliferated, for example, can be by
According to 107The viable bacteria body of acinetobacter calcoaceticus sepsis bacterium is inoculated in LB culture medium by the inoculum concentration of CFU/mL, 25-38 DEG C at a temperature of
After culture 12-48 hours, culture solution is obtained.
In the present invention, it can be commonly used in the art that for the condition of culture of acinetobacter calcoaceticus sepsis bacterium, there is no particular limitation
Various condition of culture, for example, the composition of LB liquid medium used can be with when culture are as follows: 0.8-1 weight % peptone,
0.5-0.8 weight % yeast powder, 1-1.5 weight % sodium chloride, pH=6.8-7.0.Also contain 2.5-3.0 in solid LB media
The agar of weight %.The composition of minimal medium used can be with when culture are as follows: 0.8-1.2 weight %KH2PO4, 0.8-1.2
Weight %K2HPO4, 1-1.5 weight %NH4NO3, 0.03-0.08 weight %MgSO4, 0.001-0.003 weight %CaCl2,
0.01-0.03 weight %FeSO4·7H2O, pH=6.0-7.5.
The present invention can further separate the viable bacteria body of the acinetobacter calcoaceticus sepsis bacterium in above-mentioned culture solution, the isolated side
Method is not particularly limited, as long as thallus can be enriched with from culture solution, such as can be by the side that is centrifuged and/or filters
Method realizes that the condition of the centrifugation and the filtering can be well known condition, and details are not described herein by the present invention.
The present invention further can also obtain intracellular extract from the viable bacteria body of acinetobacter calcoaceticus sepsis bacterium, for obtaining
There is no particular limitation for the method for intracellular extract, can be various methods commonly used in the art, such as can be in ice bath
Under the conditions of to thallus carry out ultrasonication, centrifuging and taking supernatant.
Second aspect, the present invention provides the acinetobacter calcoaceticus sepsis bacterium for being CGMCC NO:10855 containing deposit number
The microbial inoculum of (Acinetobacter septicus).
The present inventor is found surprisingly that in the course of the study, the dead thallus of acinetobacter calcoaceticus sepsis bacterium of the invention
Effectively heavy metal arsenic can be passivated with viable bacteria body.Therefore, the thallus of acinetobacter calcoaceticus sepsis bacterium as described above can be with
For viable bacteria body, or dead thallus can also be the mixing thallus of viable bacteria body and dead thallus.I.e. microbial inoculum contains the not lever
The dead thallus and/or viable bacteria body of bacterium sepsis bacterium.But it is more it is surprising that it was found by the inventors of the present invention that provided by the invention
The intracellular extract of acinetobacter calcoaceticus sepsis bacterium, more preferably to the passivation effect of heavy metal arsenic, it is therefore preferable that in the case of, microbial inoculum contains
There is the intracellular extract of the acinetobacter calcoaceticus sepsis bacterium.
According to the present invention, the preparation method of the above dead thallus is not particularly limited, such as, but not limited to, Ke Yitong
Nature cracking preparation is crossed, can also be prepared, can also be prepared by ultrasonication under condition of ice bath, wherein with ice by Thermal killed
Ultrasonication preparation effect is optimal under the conditions of bath.For the method that obtains intracellular extract, there is no particular limitation, Ke Yiwei
Various methods commonly used in the art, such as can be that ultrasonication, centrifuging and taking supernatant are carried out to thallus under condition of ice bath.
In the present invention, the amount of concentration or intracellular extract to the bacterium of acinetobacter calcoaceticus sepsis described in microbial inoculum is not special
It limits, can specifically be selected according to specific circumstances, in this not go into detail.
In addition, different according to scheduled purposes, microbial inoculum provided by the invention can be prepared as different dosage forms, and add phase
The ingredients such as the excipient answered.Wherein, it is known to those skilled in the art which kind of excipient is added in the microbial inoculum of which kind of dosage form,
In this not go into detail.
The third aspect, the present invention provides the application of above-mentioned acinetobacter calcoaceticus sepsis bacterium and/or microbial inoculum in passivation arsenic.It is preferred that
In the case of, arsenic source is the soil or arsenic-containing waste water of arsenic pollution.
In the present invention, " passivation arsenic ", which refers to, reduces heavy metal arsenic (As in arsenic pollution sample3+) content and its biologically effective
Property, to achieve the purpose that the environment of remediating heavy metal arsenic pollution.
Fourth aspect, the present invention provides a kind of methods for being passivated arsenic, this method comprises: being CGMCC NO by deposit number:
10855 acinetobacter calcoaceticus sepsis bacterium and/or containing deposit number be CGMCC NO:10855 acinetobacter calcoaceticus sepsis bacterium microbial inoculum with
Arsenic pollution sample contact, to be passivated to the arsenic in arsenic pollution sample.
In the method for the present invention, it can be various sides commonly used in the art that for the method for contact, there is no particular limitation
Method, such as acinetobacter calcoaceticus sepsis bacterium and/or contain that deposit number is CGMCC NO:10855 can be added in arsenic pollution sample
Deposit number is the microbial inoculum of the acinetobacter calcoaceticus sepsis bacterium of CGMCC NO:10855, is uniformly mixed.Under preferable case, the sample is soil
Earth or arsenic-containing waste water.
In the method for the present invention, for form that acinetobacter calcoaceticus sepsis bacterium into arsenic pollution sample is added, there is no special
It limits, as long as guaranteeing that the acinetobacter calcoaceticus sepsis bacterium can work and to heavy metal in the arsenic pollution sample after being added
Arsenic is effectively passivated, the form of the acinetobacter calcoaceticus sepsis bacterium of addition, for example, can for culture to logarithmic phase thallus or
Bacterium solution, or the thalli dry powder after freeze-drying can also be its intracellular extract.
The present invention is also not particularly limited the quantity of acinetobacter calcoaceticus sepsis bacterium and the amount of microbial inoculum of addition, this can root
It is determined according to the content and passivation complexity of the arsenic in the arsenic pollution sample, for example, when the arsenic content in the sample
It is higher or it is more difficult passivation or it is less favorable for the existence of the acinetobacter calcoaceticus sepsis bacterium when, the acinetobacter calcoaceticus sepsis can be improved
The inoculum concentration of bacterium and the additional amount of microbial inoculum；When the arsenic content in the sample is lower or is easier to be passivated or lose the acinetobacter calcoaceticus
When the influence of the existence of blood bacterium is smaller, it is possible to reduce the inoculum concentration of the acinetobacter calcoaceticus sepsis bacterium and the additional amount of microbial inoculum.
According to the present invention, when arsenic pollution sample is soil, in order to further promote acinetobacter calcoaceticus provided by the invention to lose
Passivation efficiency of the blood bacterium to arsenic, it is preferred that by the water content control in soil at least 15 weight %, more preferably 18-30 weight
Measure %.
Embodiment
Below will by embodiment and comparative example, the present invention will be described in detail, but be not intended to limit the present invention.
It is conventional method in that art unless otherwise specified in experimental method in following embodiment and comparative example.It is following
Experimental material used in embodiment and comparative example is unless otherwise specified to be commercially available from routine biochemistry reagent shop.
Arsenic removal rate=(handle preceding As3+As after content-processing3+Content)/handle preceding As3+Content × 100%.
Preparation example
The acinetobacter calcoaceticus sepsis bacterium that deposit number of the invention is CGMCC NO:10855 is activated 2 in LB liquid medium
Secondary, activation carries out 12 hours at 170rpm, 30 ± 1 DEG C every time, bacterium solution is obtained, by obtained bacterium solution connecing with 3 volume %
Kind amount is inoculated in 300ml LB liquid medium, is cultivated under 170rpm, 30 ± 1 DEG C of condition of culture, is entered after 2h
Logarithmic phase reaches stationary phase, bacteria concentration OD after 12h600About 6.
Embodiment 1
The oxidation for the acinetobacter calcoaceticus sepsis bacterium that the present embodiment is used to illustrate that deposit number of the invention is CGMCC NO:10855
Effect
300ml LB liquid medium is sterilized 15min at 121 DEG C, the bacterium solution that the preparation example of 1 volume % of access obtains,
170rpm, 12h is cultivated at 30 ± 1 DEG C.1ml bacterium solution is taken, according to 10-3、10-4、10-5LB solid is spread evenly across after dilution respectively
On culture medium, faint yellow circular colonies are grown after 3 days, then addition 1ml newly matches respectively in the side of LB solid medium
5 weight %AgNO of system3Solution makes AgNO3Solution covers bacterium colony respectively, stands 30min, (in Fig. 2 from left to right successively such as Fig. 2
Corresponding 10-3、10-4、10-5Bacterium colony figure after dilution) shown in, observe AgNO3Solution covers the obvious blackening in region, shows the bacterium
With very strong oxidisability.
Embodiment 2
Acinetobacter calcoaceticus sepsis bacterium of the present embodiment for illustrating deposit number of the invention for CGMCC NO:10855 is in water phase
In to the tolerance and passivation ability of arsenic
As is added into 300ml LB liquid medium3+Solution is (by As2O3It is dissolved in 37.5% HCl solution, obtains
As3+Solution), make As3+Concentration is respectively 50mg/L, 100mg/L, 200mg/L and 250mg/L, and the pH value of culture medium is adjusted to
121 DEG C of sterilizing 15min after 7.0.Preparation is accessed with the inoculum concentration of 3 volume % into the aforementioned LB liquid medium after sterilizing respectively
The bacterium solution that example obtains.170rpm, it cultivates at 30 ± 1 DEG C, bacterium solution OD is surveyed in sampling600Value and As3+Concentration and calculate As3+Removal
Rate.Wherein, As3+Concentration be measured with ICP-AES method, measuring method includes: to take appropriate bacterium solution, 8000rpm be centrifuged 5min,
Supernatant is taken, using As in ICP-AES detection supernatant3+Concentration, the condition of ICP-AES include: that absorbing wavelength is 189.0nm.
As the result is shown: when 48h, in As3+Concentration is the bacterial strain energy under 50mg/L, 100mg/L, 200mg/L and 250mg/L
The highest OD reached600Value is respectively 5.20,6.55,7.07 and 6.32, to As3+Removal rate be respectively 43.66%,
65.20%, 88.0% and 66.0%.Show deposit number of the invention be CGMCC NO:10855 acinetobacter calcoaceticus sepsis bacterium not only
It is resistant to the arsenic pollution of high concentration, and there are also higher arsenic passivation abilities.
Embodiment 3
Acinetobacter calcoaceticus sepsis bacterium of the present embodiment for illustrating deposit number of the invention for CGMCC NO:10855 is to soil
The passivation effect of middle arsenic
100ml LB liquid medium is sterilized 15min at 121 DEG C, the bacterium solution that the preparation example of 1 volume % of access obtains,
170rpm, 12h is cultivated at 30 ± 1 DEG C.Then aforementioned bacterium solution is added to 1kg heavy metal arsenic contaminated soil (As3+Content is
In 49.33mg/kg), appropriate aseptic deionized water is added, stirs evenly, cultivates 15d, guarantee that water content is in daily soil
20%.After 15 days, soil is dried at 70 DEG C, accurately weigh 0.5g, the HNO of 65% weight % of 10ml is added3Afterwards 195
Micro-wave digestion 20min at DEG C, takes supernatant liquid filtering, measures As with ICP-AES3+Content.As in soil3+It is dropped by 49.33mg/kg
To 8.26mg/kg.Show to be decreased obviously through the content of arsenic in the processed As polluted soil of this bacterial strain, illustrates the bacterial strain to soil
The passivation of middle heavy metal arsenic has good effect, can be effectively reduced the bioavailability of arsenic in the soil.
Embodiment 4
Acinetobacter calcoaceticus sepsis bacterium of the present embodiment for illustrating deposit number of the invention for CGMCC NO:10855 is in water phase
Passivation effect of the middle different shape to arsenic
100ml LB liquid medium is sterilized 15min at 121 DEG C, then accesses what the preparation example of 1 volume % obtained
170rpm, bacterium solution cultivates 12h at 30 ± 1 DEG C.Bacterium solution is divided into 2 parts, a 4 DEG C of centrifuging and taking supernatants as go up final proof
Product；Another 121 DEG C sterilizing 15min, then by 4 DEG C of centrifuging and taking supernatants of bacterium solution, as high-temperature sterilization sample.Use 10mmol/L
It is resuspended after thallus 2 times that Tris-HCI buffer (pH 7.0) washing centrifugation corresponding with Supernatant samples obtains, then in ice bath
Under the conditions of ultrasonication, supersonic frequency 25KHz, ultrasonic time 20min, 4 DEG C of centrifuging and taking supernatants, as intracellular extract
Sample.3 parts of samples are separately added into a certain amount of As3+Solution is (by As2O3It is dissolved in 37.5% HCl solution, obtains As3+It is molten
Liquid), make As3+Concentration is 200mg/L, and adjusting sample final ph is 7.0.It is centrifuged after 48h and measures the As in supernatant3+
Concentration, As3+The results are shown in Table 1 for removal rate.
Table 1
| Project | As3+Removal rate |
| Supernatant samples | 32.8% |
| High-temperature sterilization sample | 22.4% |
| Intracellular extract sample | 87.5% |
As can be seen from Table 1, intracellular extract sample is to As3+Removal rate is up to 87.5%, much higher than Supernatant samples and
High-temperature sterilization sample shows that the intracellular extract sample of the acinetobacter calcoaceticus sepsis bacterium of CGMCC NO:10855 of the invention can
More effectively passivation arsenic.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (8)
1. a kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus), which is characterized in that the acinetobacter calcoaceticus sepsis bacterium
Deposit number be CGMCC NO:10855.
2. a kind of microbial inoculum, which is characterized in that the microbial inoculum contains acinetobacter calcoaceticus sepsis bacterium described in claim 1
(Acinetobacter septicus)。
3. microbial inoculum according to claim 2, wherein the microbial inoculum contain the acinetobacter calcoaceticus sepsis bacterium dead thallus and/or
Viable bacteria body.
4. microbial inoculum according to claim 2, wherein the microbial inoculum contains the intracellular extraction of the acinetobacter calcoaceticus sepsis bacterium
Object.
5. microbial inoculum described in any one of acinetobacter calcoaceticus sepsis bacterium described in claim 1 and/or claim 2-4 is being passivated
Application in arsenic.
6. application according to claim 5, wherein arsenic source is the soil or arsenic-containing waste water of arsenic pollution.
7. a kind of method for being passivated arsenic, which is characterized in that the described method includes: by acinetobacter calcoaceticus sepsis described in claim 1
Microbial inoculum described in any one of bacterium and/or claim 2-4 is contacted with arsenic pollution sample, to the arsenic in arsenic pollution sample
It is passivated.
8. according to the method described in claim 7, wherein, the sample is soil or arsenic-containing waste water.
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| CN101974448A (en) * | 2010-09-07 | 2011-02-16 | 新疆农业科学院微生物应用研究所 | Acinetobacter and application thereof to biological treatment of heavy metal ions |
| CN102634465A (en) * | 2011-12-27 | 2012-08-15 | 西北大学 | Acinetobacter with authigenic nitrogen fixation capacity and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101974448A (en) * | 2010-09-07 | 2011-02-16 | 新疆农业科学院微生物应用研究所 | Acinetobacter and application thereof to biological treatment of heavy metal ions |
| CN102634465A (en) * | 2011-12-27 | 2012-08-15 | 西北大学 | Acinetobacter with authigenic nitrogen fixation capacity and application thereof |
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