CN106497814A - A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for passivation arsenic - Google Patents
A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for passivation arsenic Download PDFInfo
- Publication number
- CN106497814A CN106497814A CN201510564518.2A CN201510564518A CN106497814A CN 106497814 A CN106497814 A CN 106497814A CN 201510564518 A CN201510564518 A CN 201510564518A CN 106497814 A CN106497814 A CN 106497814A
- Authority
- CN
- China
- Prior art keywords
- arsenic
- bacterium
- sepsis
- acinetobacter calcoaceticus
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to the technical field of biological remediation of heavy metal arsenic pollution, discloses a kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and a kind of method of passivation arsenic, wherein, the preserving number of the acinetobacter calcoaceticus sepsis bacterium is CGMCC NO：10855.The method of the passivation arsenic of the present invention includes：It is CGMCC NO by preserving number：10855 acinetobacter calcoaceticus sepsis bacterium and/or containing preserving number be CGMCC NO：The microbial inoculum of 10855 acinetobacter calcoaceticus sepsis bacterium is contacted with arsenic pollution sample, to be passivated to the arsenic in arsenic pollution sample.The acinetobacter calcoaceticus sepsis bacterium of the present invention has higher arsenic passivation ability, can tolerate the arsenic pollution of high concentration, easy to operate, can the soil of arsenic pollution or arsenic-containing waste water biological prosthetic during play a significant role.
Description
Technical field
The present invention relates to the technical field of biological remediation of heavy metal arsenic pollution, in particular it relates to a kind of motionless
Bacillus sepsis bacterium, the microbial inoculum containing the bacterium and its application and a kind of method of passivation arsenic.
Background technology
Arsenic (As) is one of five big hypertoxic elements.It is big that arsenic in soil is mainly derived from natural and artificial two
Approach.Natural origin is mainly the contained arsenic element of soil itself, in addition to rich arsenic area, most
Arsenic in soil background value is about 15mg/kg.Soil arsenic pollution is mainly derived from mankind's activity.Arsenic element and its
Derivative is widely used in manufacturing industry, such as electronic product, alloy and coating etc..Metal mining simultaneously
Substantial amounts of arsenic can also be discharged in soil and atmospheric environment with coal mining activity.Used in agricultural production
Agricultural chemicals containing arsenic and chemical fertilizer are also class important sources of Arsenic in Soil.Arsenide was once widely used in agricultural
Make insecticide, thimerosal, bactericide and herbicide.In addition, also containing a certain amount of arsenic in some chemical fertilizer.
The arsenic of 20-50mg/kg is typically contained in phosphate fertilizer, and the arsenic content wherein in composite fertilizer is higher, chronic administration
The arsenic element of enriched in soil is made, causes severe contamination.Inorganic arsenic chemicals is persistently deposited in soil
, and can be utilized by plant, and then poisonous plant affects plant growth.
In view of the characteristic of arsenic itself, it is necessary to manually administered and repaired.The method that can be adopted includes
Physical method, chemical method and biological method, have the following aspects：Immobilization/stabilization technology,
Vitrification, Soil leaching technology, electric repairing technique in situ.Above-mentioned restorative procedure can different journeys
Degree ground destruction ecological environment of soil.That is, currently used physics, chemical remediation technology are not only
Complex operation, and institute can cause irreversible breaking using chemical reagent or physical operations to soil environment
Bad.Conversely, bioremediation technology repairing effect is good, reduced investment, be easy to implement, do not produce secondary pollution.
Therefore, in order to keep the activity of soil texture and microorganism, filter out in efficient passivation bacterial strain passivation soil
Arsenic be current problem demanding prompt solution.
Content of the invention
The invention aims to overcome arsenic pollution biological prosthetic present in drawbacks described above, there is provided a kind of
Acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and a kind of method of passivation arsenic.The present invention's
Acinetobacter calcoaceticus sepsis bacterium, with higher arsenic passivation ability, can tolerate the arsenic pollution of high concentration, operation letter
Just, can the soil of arsenic pollution or arsenic-containing waste water biological prosthetic during play a significant role.
To achieve these goals, the present inventor has carried out substantial amounts of screening experiment, as a result screens
To a kind of acinetobacter calcoaceticus sepsis bacterium with higher arsenic passivation ability, the arsenic pollution of tolerable high concentration.
Therefore, in a first aspect, the invention provides a kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus),
The preserving number of the acinetobacter calcoaceticus sepsis bacterium is CGMCC NO：10855.
Second aspect, the invention provides a kind of contain above-mentioned acinetobacter calcoaceticus sepsis bacterium (Acinetobacter
Septicus microbial inoculum).
The third aspect, the invention provides above-mentioned acinetobacter calcoaceticus sepsis bacterium and/or microbial inoculum are in passivation arsenic
Application.
Fourth aspect, the invention provides a kind of method of passivation arsenic, methods described includes：By above-mentioned not
Lever bacterium sepsis bacterium and/or above-mentioned microbial inoculum are contacted with arsenic pollution sample, to enter to the arsenic in arsenic pollution sample
Row passivation.
The acinetobacter calcoaceticus sepsis bacterium of the present invention is heavy metal arsenic efficient passivation bacterial strain, can reduce arsenic in water phase
Or the bioavailability in soil phase.Compared with prior art, acinetobacter calcoaceticus sepsis bacterium energy of the invention
Arsenic in enough efficient passivations soil phase (little to soil disturbance) or water phase, can tolerate the arsenic pollution of high concentration,
Easy to operate, can the soil of arsenic pollution or arsenic-containing waste water biological prosthetic during play a significant role,
Realize the purpose that environment-friendly and green is repaired.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Description of the drawings
The single bacterium colony figure that Fig. 1 is obtained for plate streaking.
Fig. 2 is AgNO3Solution covers the bacterium colony design sketch after acinetobacter calcoaceticus sepsis bacterium bacterium colony.
Biological deposits
The acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus) of the present invention, in May 22 in 2015
Day is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address：Beijing
The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode：100101)
(depositary institution is abbreviated as CGMCC), deposit number is CGMCC：10855.
Specific embodiment
Hereinafter the specific embodiment of the present invention is described in detail.It should be appreciated that this place is retouched
The specific embodiment that states is merely to illustrate and explains the present invention, is not limited to the present invention.
In a first aspect, the invention provides a kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus),
The preserving number of the acinetobacter calcoaceticus sepsis bacterium is CGMCC NO：10855.
The preserving number of the present invention is CGMCC NO：10855 acinetobacter calcoaceticus sepsis bacterium, is gram
Negative bacterium, belongs to Moraxella Cordycepps acinetobacter sepsis bacterium.The bacterium source is in Xuancheng Profile, anhui Province arsenic pollution
Soil, screens and passes through dilution plate method and isolate and purify to obtain using soil circulating current system.Biological characteristics
Property includes：Morphological feature be on LB solid mediums bacterium colony be faint yellow, circular, neat in edge,
Surface wettability is smooth, and micro- sem observation is shaft-like in tubbiness, atrichia；Gram stain test, amylase
Experiment, oxidase test, nitrate reduction test, product H2S experiments are negative, gelatin liquefaction test, connect
Catalase test is positive, it is impossible to using mannose.
Wherein, the separation process of acinetobacter calcoaceticus sepsis bacterium of the invention can include：By 100g soil with
The sand grains that appropriate particle diameter is about 3mm is well mixed, and is placed in the upper strata of circulation enriching apparatus, 200ml LB
Culture medium is placed in lower floor as circulation liquid.Start peristaltic pump, according to the evaporation feelings of circulation liquid in enrichment process
Condition periodically adds circulation liquid.After enrichment terminates, taking upper layer of soil, to carry out plate streaking with lower floor circulation liquid pure
Change and separate：Supernatant is diluted 10 respectively-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8,
Draw coat containing 25 in right amount, 50,100,150,200,400mg/L As3+LB solids training
On foster base, 25-30 DEG C of culture takes bacterium colony after 3 days and carries out plate streaking, separates single bacterium colony, such as Fig. 1 institutes
Show.
The present invention obtains one plant of most strong bacterial strain of heavy metal arsenic resistance from the bacterial strain for filtering out
As T, carry out Physiology and biochemistry identification and analysis to the bacterial strain, and its biological characteristics includes：Morphological feature be
On LB solid mediums, bacterium colony is that faint yellow, circular, neat in edge, surface wettability are smooth, microscope
Observation is shaft-like in tubbiness, atrichia；Gram stain test, amylase experiment, oxidase test, nitre
Hydrochlorate reduction test, product H2S experiments are negative, and gelatin liquefaction test, catalase test are positive, it is impossible to profit
Use mannose.Meanwhile, according to Tiangeng bacterial genomes DNA extraction kit (TIANamp bacteria
DNA kit) step extracts the DNA of the bacterial strain, carries out 16S rDNA gene sequencings, its 16s
As a result rDNA gene orders show that the bacterium is acinetobacter calcoaceticus sepsis bacterium as shown in SEQ ID NO.1
(Acinetobacter septicus).The acinetobacter calcoaceticus sepsis bacterium bacterium is preserved in Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, preserving number is CGMCC NO：10855.
The acinetobacter calcoaceticus sepsis bacterium that the present invention is provided can produce a large amount of acinetobacter calcoaceticus sepsis bacterium through culture
Viable bacteria body, the method for the culture is not particularly required, as long as the acinetobacter calcoaceticus sepsis can be made
Bacterium breeds, for example, it is possible to according to 107The inoculum concentration of CFU/mL is by the work of acinetobacter calcoaceticus sepsis bacterium
Thalline is inoculated in LB culture mediums, after cultivating 12-48 hours, is cultivated at a temperature of 25-38 DEG C
Liquid.
In the present invention, for the condition of culture of acinetobacter calcoaceticus sepsis bacterium, there is no particular limitation, can be this
The conventional various condition of culture in field, for example, the composition of LB fluid nutrient mediums used during culture can be with
For：0.8-1 weight % peptones, 0.5-0.8 weight % dusty yeasts, 1-1.5 weight % sodium chloride,
PH=6.8-7.0.Agar in solid LB media also containing 2.5-3.0 weight %.Used during culture
The composition of minimal medium can be：0.8-1.2 weight %KH2PO4, 0.8-1.2 weight %K2HPO4,
1-1.5 weight %NH4NO3, 0.03-0.08 weight %MgSO4, 0.001-0.003 weight %CaCl2,
0.01-0.03 weight %FeSO4·7H2O, pH=6.0-7.5.
The present invention can further separate the viable bacteria body of the acinetobacter calcoaceticus sepsis bacterium in above-mentioned nutrient solution, described
Detached method has no particular limits, as long as thalline can be enriched with from nutrient solution, for example can be with
Realize that the condition of the centrifugation and the filtration can be known by the method being centrifuged and/or filter
Condition, the present invention will not be described here.
The present invention further can also obtain intracellular extract from the viable bacteria body of acinetobacter calcoaceticus sepsis bacterium,
For the method for obtaining intracellular extract, there is no particular limitation, can be various sides commonly used in the art
Method, for example, can be to carry out ultrasonication, centrifuging and taking supernatant under condition of ice bath to thalline.
Second aspect, the invention provides be CGMCC NO containing preserving number：10855 not lever
The microbial inoculum of bacterium sepsis bacterium (Acinetobacter septicus).
The present inventor is found surprisingly that in the course of the study, the acinetobacter calcoaceticus sepsis bacterium of the present invention
Dead thalline and viable bacteria body effectively heavy metal arsenic can be passivated.Therefore, motionless as above
The thalline of bacillus sepsis bacterium can be viable bacteria body, or dead thalline, can also be viable bacteria body and dead bacterium
The mixing thalline of body.I.e. microbial inoculum contains the dead thalline and/or viable bacteria body of the acinetobacter calcoaceticus sepsis bacterium.But
More it is surprising that it was found by the inventors of the present invention that the present invention provide acinetobacter calcoaceticus sepsis bacterium thin
Intracellular extract, the passivation effect of heavy metal arsenic more preferably, it is therefore preferable that in the case of, microbial inoculum is containing
State the intracellular extract of acinetobacter calcoaceticus sepsis bacterium.
According to the present invention, for the preparation method of above dead thalline has no particular limits, for example but do not limit
In, can by naturally cracking prepare, it is also possible to prepared by Thermal killed, condition of ice bath can also be passed through
Prepared by lower ultrasonication, wherein prepare effect with ultrasonication under condition of ice bath optimum.For obtaining cell
There is no particular limitation for the method for interior extract, can be various methods commonly used in the art, for example can be with
It is to carry out ultrasonication, centrifuging and taking supernatant under condition of ice bath to thalline.
In the present invention, the concentration of the bacterium of acinetobacter calcoaceticus sepsis described in microbial inoculum or the amount of intracellular extract are not had
There is special restriction, specifically can be selected according to specific circumstances, in this not go into detail.
In addition, different according to predetermined purposes, the microbial inoculum that the present invention is provided can be prepared as different formulations,
And add the compositions such as corresponding excipient.Wherein, add which kind of excipient is in the microbial inoculum of which kind of formulation
Known to one of skill in the art, in this not go into detail.
The third aspect, the invention provides above-mentioned acinetobacter calcoaceticus sepsis bacterium and/or microbial inoculum are in passivation arsenic
Application.Under preferable case, soil or arsenic-containing waste water of the arsenic source for arsenic pollution.
In the present invention, " passivation arsenic " refers to heavy metal arsenic (As in reduction arsenic pollution sample3+) content and
Its biological effectiveness, so that reach the purpose of the environment of remediating heavy metal arsenic pollution.
Fourth aspect, the invention provides a kind of method of passivation arsenic, the method includes：By preserving number it is
CGMCC NO：10855 acinetobacter calcoaceticus sepsis bacterium and/or containing preserving number be CGMCC NO：
The microbial inoculum of 10855 acinetobacter calcoaceticus sepsis bacterium is contacted with arsenic pollution sample, with to the arsenic in arsenic pollution sample
It is passivated.
In the inventive method, for the method for contacting, there is no particular limitation, can be commonly used in the art
Various methods, it is CGMCC NO that for example can add preserving number in arsenic pollution sample：10855
Acinetobacter calcoaceticus sepsis bacterium and/or containing preserving number be CGMCC NO：10855 acinetobacter calcoaceticus sepsis
The microbial inoculum of bacterium, is well mixed.Under preferable case, the sample is soil or arsenic-containing waste water.
In the inventive method, for add to the acinetobacter calcoaceticus sepsis bacterium in arsenic pollution sample form not
There is special restriction, as long as the acinetobacter calcoaceticus sepsis bacterium can be in the arsenic pollution sample after ensureing to add
In work and heavy metal arsenic is effectively passivated, the shape of the acinetobacter calcoaceticus sepsis bacterium of addition
Formula, for example, it is possible to be thalline or the bacterium solution that cultivates to logarithmic phase, or the thalline after freeze-drying
Dry powder, can also be its intracellular extract.
The present invention is also had no particular limits to the quantity of acinetobacter calcoaceticus sepsis bacterium and the amount of microbial inoculum of addition,
This according to the content of the arsenic in the arsenic pollution sample and can be passivated complexity determine, for example,
When the arsenic content in the sample is higher or more difficult passivation or existence for the acinetobacter calcoaceticus sepsis bacterium
When less favorable, the inoculum concentration of the acinetobacter calcoaceticus sepsis bacterium and the addition of microbial inoculum can be improved；When described
Arsenic content in sample relatively low be easier to be passivated or the impact to the existence of the acinetobacter calcoaceticus sepsis bacterium compared with
Hour, it is possible to reduce the addition of the inoculum concentration of the acinetobacter calcoaceticus sepsis bacterium and microbial inoculum.
According to the present invention, when arsenic pollution sample is soil, in order to further promote the present invention to provide not
Passivation efficiency of the lever bacterium sepsis bacterium to arsenic, it is preferred that control the water content in soil at least 15
Weight %, more preferably 18-30 weight %.
Embodiment
Hereinafter will be described the present invention by embodiment and comparative example, but and be not so limited this
Invention.
In experimental technique in following examples and comparative example, if no special instructions, this area routine is
Method.Experiment material used in following embodiments and comparative example, if no special instructions, is from routine
Biochemical reagents shop is commercially available.
Arsenic removal rate=(before processing As3+As after content-process3+Content)/before processing As3+Content × 100%.
Preparation example
It is CGMCC NO by the preserving number of the present invention：10855 acinetobacter calcoaceticus sepsis bacterium is in LB liquid
Activate 2 times in culture medium, activation every time is carried out at 170rpm, 30 ± 1 DEG C 12 hours, obtains bacterium
Liquid, the bacterium solution for obtaining is inoculated in 300ml LB fluid nutrient mediums with the inoculum concentration of 3 volumes %,
Cultivated under 170rpm, 30 ± 1 DEG C of condition of culture, after 2h, entered logarithmic phase, reached after 12h steady
Periodically, bacteria concentration OD600About 6.
Embodiment 1
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：10855 acinetobacter calcoaceticus lose
The oxidation effectiveness of blood bacterium
300ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, access the preparation example of 1 volume %
The bacterium solution for obtaining, 170rpm, cultivates 12h at 30 ± 1 DEG C.1ml bacterium solutions are taken, according to 10-3、10-4、
10-5It is spread evenly across after dilution respectively on LB solid mediums, after 3 days, grows faint yellow circular colonies,
Then 5 weight %AgNO that 1ml is newly prepared respectively are added in the side of LB solid mediums3Solution,
Make AgNO3Solution covers bacterium colony respectively, stands 30min, and such as Fig. 2 is (from left to right right successively in Fig. 2
Answer 10-3、10-4、10-5Bacterium colony figure after dilution) shown in, observe AgNO3It is bright that solution covers region
Aobvious blackening, shows that the bacterium has very strong oxidisability.
Embodiment 2
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：10855 acinetobacter calcoaceticus lose
Tolerance and passivation ability of the blood bacterium in water phase to arsenic
As is added in 300ml LB fluid nutrient mediums3+Solution is (by As2O3It is dissolved in 37.5% HCl
In solution, As is obtained3+Solution), make As3+Concentration is respectively 50mg/L, 100mg/L, 200mg/L
And 250mg/L, 121 DEG C of sterilizing 15min after the pH value of culture medium is adjusted to 7.0.Respectively to going out
The bacterium solution that preparation example is obtained is accessed with the inoculum concentration of 3 volumes % in aforementioned LB fluid nutrient mediums after bacterium.
170rpm, cultivate at 30 ± 1 DEG C, bacterium solution OD is surveyed in sampling600Value and As3+Concentration and calculate As3+Go
The rate of removing.Wherein, As3+Concentration be measured with ICP-AES methods, assay method includes：Take appropriate bacterium
Liquid, 8000rpm are centrifuged 5min, take supernatant, using As in ICP-AES detection supernatants3+Concentration,
The condition of ICP-AES includes：Absorbing wavelength is 189.0nm.
As a result show：During 48h, in As3+Concentration is 50mg/L, 100mg/L, 200mg/L and 250mg/L
Under, highest OD that the bacterial strain can reach600Value is respectively 5.20,6.55,7.07 and 6.32, to As3+
Clearance be respectively 43.66%, 65.20%, 88.0% and 66.0%.Show the preserving number of the present invention
For CGMCC NO：10855 acinetobacter calcoaceticus sepsis bacterium can not only enduring high-concentration arsenic pollution,
And also have higher arsenic passivation ability.
Embodiment 3
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：10855 acinetobacter calcoaceticus
Passivation effect of the sepsis bacterium to Arsenic in Soil
100ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, access the preparation example of 1 volume %
The bacterium solution for obtaining, 170rpm, cultivates 12h at 30 ± 1 DEG C.Then aforementioned bacterium solution is added to 1kg weights
Metal As polluted soil (As3+Content is 49.33mg/kg) in, appropriate aseptic deionized water is added, is stirred
Mix uniform, culture 15d, it is ensured that water content is 20% in soil daily.After 15 days, by soil at 70 DEG C
Lower drying, accurately weighs 0.5g, adds the HNO of 65% weight % of 10ml3At 195 DEG C, microwave disappears afterwards
Solution 20min, takes supernatant liquid filtering, determines As with ICP-AES3+Content.As in soil3+By
49.33mg/kg drop to 8.26mg/kg.Show the content through arsenic in the As polluted soil that this bacterial strain was processed
It is decreased obviously, illustrates that the bacterial strain has good effect to the passivation of heavy metal in soil arsenic, can be effective
Reduce bioavailability of the arsenic in soil.
Embodiment 4
The present embodiment is used for illustrating that the preserving number of the present invention to be CGMCC NO：10855 acinetobacter calcoaceticus
The sepsis bacterium passivation effect of different shape to arsenic in water phase
100ml LB fluid nutrient mediums are sterilized at 121 DEG C 15min, then access the system of 1 volume %
The bacterium solution that standby example is obtained, 170rpm, cultivates 12h at 30 ± 1 DEG C.Bacterium solution is divided into 2 parts, one
4 DEG C of centrifuging and taking supernatants of part, as Supernatant samples；Another 121 DEG C sterilizing 15min, then by bacterium solution 4
DEG C centrifuging and taking supernatant, as high-temperature sterilization sample.With 10mmol/L Tris-HCI buffer solutions (pH 7.0)
Washing is corresponding with Supernatant samples be centrifuged resuspended after the thalline that obtains 2 times, then ultrasonic under condition of ice bath
Broken, supersonic frequency is 25KHz, and ultrasonic time is 20min, 4 DEG C of centrifuging and taking supernatants, as cell
Interior extract sample.3 parts of samples are separately added into a certain amount of As3+Solution is (by As2O3It is dissolved in 37.5%
HCl solution in, obtain As3+Solution), make As3+Concentration is 200mg/L, and it is final to adjust sample
PH value is 7.0.The As for being centrifuged after 48h and determining in supernatant3+Concentration, As3+Clearance result such as table
Shown in 1.
Table 1
| Project | As3+Clearance |
| Supernatant samples | 32.8% |
| High-temperature sterilization sample | 22.4% |
| Intracellular extract sample | 87.5% |
As can be seen from Table 1, intracellular extract sample is to As3+Clearance is up to 87.5%, is far above
Supernatant samples and high-temperature sterilization sample, show the CGMCC NO of the present invention：10855 acinetobacter calcoaceticus
The intracellular extract sample of sepsis bacterium can more effectively be passivated arsenic.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited to above-mentioned reality
The detail in mode is applied, in the range of the technology design of the present invention, can be to the technical side of the present invention
Case carries out multiple simple variants, and these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique described in above-mentioned specific embodiment is special
Levy, in the case of reconcilable, can be combined by any suitable means, in order to avoid need not
The repetition that wants, the present invention are no longer separately illustrated to various possible combinations.
Additionally, can also be combined between a variety of embodiments of the present invention, as long as its
Without prejudice to the thought of the present invention, which should equally be considered as content disclosed in this invention.
Claims (8)
1. a kind of acinetobacter calcoaceticus sepsis bacterium (Acinetobacter septicus), it is characterised in that this is motionless
The preserving number of bacillus sepsis bacterium is CGMCC NO：10855.
2. a kind of microbial inoculum, it is characterised in that the microbial inoculum contains the acinetobacter calcoaceticus described in claim 1 and loses
Blood bacterium (Acinetobacter septicus).
3. microbial inoculum according to claim 2, wherein, the microbial inoculum contains the acinetobacter calcoaceticus sepsis
The dead thalline and/or viable bacteria body of bacterium.
4. microbial inoculum according to claim 2, wherein, the microbial inoculum contains the acinetobacter calcoaceticus sepsis
The intracellular extract of bacterium.
5. any one institute in acinetobacter calcoaceticus sepsis bacterium described in claim 1 and/or claim 2-4
Application of the microbial inoculum that states in passivation arsenic.
6. application according to claim 5, wherein, arsenic source is the soil of arsenic pollution or useless containing arsenic
Water.
7. a kind of passivation arsenic method, it is characterised in that methods described includes：By claim 1 institute
The acinetobacter calcoaceticus sepsis bacterium that states, and/or the microbial inoculum in claim 2-4 described in any one and arsenic pollution sample
Product are contacted, to be passivated to the arsenic in arsenic pollution sample.
8. method according to claim 7, wherein, the sample is soil or arsenic-containing waste water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510564518.2A CN106497814B (en) | 2015-09-07 | 2015-09-07 | A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for being passivated arsenic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510564518.2A CN106497814B (en) | 2015-09-07 | 2015-09-07 | A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for being passivated arsenic |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106497814A true CN106497814A (en) | 2017-03-15 |
| CN106497814B CN106497814B (en) | 2019-05-10 |
Family
ID=58286633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510564518.2A Expired - Fee Related CN106497814B (en) | 2015-09-07 | 2015-09-07 | A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for being passivated arsenic |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106497814B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140846A (en) * | 2022-07-06 | 2022-10-04 | 江苏聚庚科技有限公司 | Composite treating agent, preparation method and application thereof in wastewater purification |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974448A (en) * | 2010-09-07 | 2011-02-16 | 新疆农业科学院微生物应用研究所 | Acinetobacter and application thereof to biological treatment of heavy metal ions |
| CN102634465A (en) * | 2011-12-27 | 2012-08-15 | 西北大学 | Acinetobacter with authigenic nitrogen fixation capacity and application thereof |
-
2015
- 2015-09-07 CN CN201510564518.2A patent/CN106497814B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974448A (en) * | 2010-09-07 | 2011-02-16 | 新疆农业科学院微生物应用研究所 | Acinetobacter and application thereof to biological treatment of heavy metal ions |
| CN102634465A (en) * | 2011-12-27 | 2012-08-15 | 西北大学 | Acinetobacter with authigenic nitrogen fixation capacity and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 杨宇: "湖南石门雄黄矿区矿样中的砷氧化细菌的分离及鉴定", 《生态环境学报》 * |
| 杨莉: "雄黄矿区微生物多样性分析及砷氧化菌株的筛选与应用", 《中国硕士学位论文工程科技Ⅰ辑》 * |
| 蒋友芬: "新疆奎屯地区高砷环境中抗砷菌的初步筛选", 《中国硕士学位论文 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140846A (en) * | 2022-07-06 | 2022-10-04 | 江苏聚庚科技有限公司 | Composite treating agent, preparation method and application thereof in wastewater purification |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106497814B (en) | 2019-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104450552B (en) | A kind of sulfate reducing bacteria phosphate solubilizing bacteria and its application in combined repair of cadmium polluted soil | |
| CN106434374B (en) | Trichoderma asperellum and its application in heavy metal pollution reparation | |
| CN106493167B (en) | Bacillus licheniformis and microbial inoculum and their application and heavy metal-passivated method | |
| Tao et al. | Application of biochar with functional microorganisms for enhanced atrazine removal and phosphorus utilization | |
| CN102757915B (en) | Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof | |
| CN105670980B (en) | A kind of application of the microbial strains of restoration of soil polluted by heavy metal | |
| CN104403978B (en) | Rhodopseudomonas palustris bacterial strain, the preparation method of microbial inoculum and microbial inoculum, extracellular protein and its extracting method and application | |
| CN104531585B (en) | A kind of Dell's Ford bacterium and its application | |
| CN106167776A (en) | A kind of can bacillus cereus (Bacillus cereus) TH 35 of heavy metal cadmium and application thereof in activating soil | |
| CN106497809A (en) | A kind of enterobacter cloacae, the microbial inoculum containing the bacterium and its application and the method for passivation hydrargyrum | |
| CN109207404A (en) | Siam bacillus YJ15 and its application | |
| CN107460146A (en) | The application of Alcaligenes faecalis excrement sp. strain, enzyme preparation and its degrading pesticide residues | |
| CN106244500B (en) | One plant of anti-antimony bacterium NXH2 and its application | |
| CN106148232A (en) | The bacterial isolates of one strain antagonism plant pathogenetic bacteria and application thereof | |
| CN104673715B (en) | There is fixed effect to cadmium and enterobacteria and its application of plant growth can be promoted | |
| CN104531583B (en) | A kind of pseudomonad and the microbial flocculant obtained in it ferments | |
| CN106190871A (en) | A kind of method that compound thread fungal organism drip leaching with straw as carbon source processes heavy-metal contaminated soil | |
| CN111004736A (en) | Bacillus megaterium and application thereof in degrading pyrethroid insecticides | |
| CN106244501B (en) | One plant of anti-antimony bacterium NXH1 and its application | |
| CN109516869A (en) | Complex function microorganism formulation and its preparation method and application | |
| CN106434446B (en) | One plant of anti-antimony bacterium NXH3 and its application | |
| CN104818234A (en) | Streptomycete with cadmium tolerant characteristic and application thereof | |
| Samah et al. | Production of plant growth regulators by some fungi isolated under salt stress | |
| CN106591173A (en) | Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof | |
| CN106497814A (en) | A kind of acinetobacter calcoaceticus sepsis bacterium, the microbial inoculum containing the bacterium and its application and the method for passivation arsenic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190510 Termination date: 20190907 |