CN106367363B - Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment - Google Patents
Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment Download PDFInfo
- Publication number
- CN106367363B CN106367363B CN201610078160.7A CN201610078160A CN106367363B CN 106367363 B CN106367363 B CN 106367363B CN 201610078160 A CN201610078160 A CN 201610078160A CN 106367363 B CN106367363 B CN 106367363B
- Authority
- CN
- China
- Prior art keywords
- microbial inoculum
- hexavalent chromium
- fermentation
- percent
- polluted water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 238000000855 fermentation Methods 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 41
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 241000946784 Streptomyces puniceus Species 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 235000013379 molasses Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 3
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 241000187095 Streptomyces purpureus Species 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 239000010865 sewage Substances 0.000 abstract description 3
- 239000011651 chromium Substances 0.000 description 34
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 27
- 229910052804 chromium Inorganic materials 0.000 description 26
- 229910001385 heavy metal Inorganic materials 0.000 description 17
- 239000000047 product Substances 0.000 description 11
- 239000002351 wastewater Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000009713 electroplating Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 3
- 241001655322 Streptomycetales Species 0.000 description 3
- 229910001430 chromium ion Inorganic materials 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 231100000315 carcinogenic Toxicity 0.000 description 2
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 2
- AYTAKQFHWFYBMA-UHFFFAOYSA-N chromium dioxide Chemical compound O=[Cr]=O AYTAKQFHWFYBMA-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- ROORDVPLFPIABK-UHFFFAOYSA-N diphenyl carbonate Chemical compound C=1C=CC=CC=1OC(=O)OC1=CC=CC=C1 ROORDVPLFPIABK-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- JHWIEAWILPSRMU-UHFFFAOYSA-N 2-methyl-3-pyrimidin-4-ylpropanoic acid Chemical compound OC(=O)C(C)CC1=CC=NC=N1 JHWIEAWILPSRMU-UHFFFAOYSA-N 0.000 description 1
- 241001156739 Actinobacteria <phylum> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000186984 Kitasatospora aureofaciens Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204060 Streptomycetaceae Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- -1 however Chemical compound 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003295 industrial effluent Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000013048 microbiological method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a production method of a Streptomyces purpureus (Streptomyces purpureus strain LYzhlm CGMCC No.11773) microbial inoculum and a use method for treating hexavalent chromium polluted water by using the product. The experiment for treating the hexavalent chromium polluted water by using the product can reduce the concentration of hexavalent chromium in the sewage from 1790mg/L to 0.08mg/L, completely reaches the discharge standard of hexavalent chromium and has the removal rate of 100 percent. The invention also discloses a production process of the strain, and a use condition and a method for treating hexavalent chromium polluted water, wherein the process is simple, has no secondary pollution compared with a chemical method, is convenient to use, and has wide market prospect.
Description
Technical Field
The invention relates to the technical field of chromium pollution treatment, in particular to a preparation method and a use method of a microbial inoculum for treating hexavalent chromium pollution in a drainage basin.
Background
Chromium is a silvery-white hard metal, and has divalent, trivalent, and hexavalent compounds. The industrial application of chromium is wide, and mainly comprises metal processing, electroplating and leather making industries, and waste water and waste gas discharged by the industries are main pollution sources in polluted environment of drainage basins. Hexavalent chromium is highly toxic, and is a swallow poison/inhalation poison, and skin contact may cause allergy; more likely to cause genetic defects, inhalation may be carcinogenic, and there is a persistent risk to the environment. These are characteristic of hexavalent chromium, however, chromium metal, trivalent or tetravalent chromium does not have these toxicities.
The most common chromium pollution is water pollution, such as wastewater from chromium electroplating, and wastewater from industrial enterprises using chromium and its compounds in the tanning, pharmaceutical, and printing and dyeing industries, which mainly enters the environment in two valence states of Cr (III) and Cr (VI). According to the data, the tanning industry generally processes 1t of hides and discharges 50-60t of wastewater containing 410mg/L of chromium. The circulating cooling water used in oil refineries and chemical plants also has a high chromium content. The chromium content in the wastewater of the chromium plating plant is higher, and particularly, a large amount of chromium-containing wastewater is discharged when the electroplating solution is replaced. The pollution of chromium to water bodies is quite serious not only in China but also in all countries around the world. Chromium pollution is generally regarded as a key prevention and treatment object in all countries in the world. And the chromium slag is sodium dichromate and waste slag discharged in the production of metal chromium. When the chromium slag is stockpiled in the open air, a large amount of hexavalent chromium ions are dissolved, lost and seeped into the ground surface along with rainwater after long-term rainwater washing, so that underground water is polluted, rivers and lakes are polluted, and farmlands, aquatic products and human health are further harmed. An excess (over 10ppm) of hexavalent chromium is lethal to aquatic life. Experiments have shown that hexavalent chromium in contaminated drinking water can be carcinogenic. Hexavalent chromium compounds are commonly used in electroplating and the like, and are absorbed by cells of many tissues and organs in the body after animals drink water containing hexavalent chromium.
The bioremediation mechanism of the heavy metal polluted water body mainly comprises the fixation of heavy metals and the transformation of forms by microorganisms. The former is that the microorganism absorbs heavy metal ions through the charged cell surface, or actively absorbs the heavy metal ions by taking necessary nutrient elements, so as to enrich the heavy metal on the cell surface or inside the cell; the latter is to reduce heavy metal pollution by changing the form of heavy metals or reducing the bioavailability of heavy metals through the life activities of microorganisms, such as Cr6+Conversion to Cr3+And the toxicity is reduced, As, Hg, Se and the like are reduced into simple substances and volatilized, and the secretion of microorganisms has passivation effect on heavy metals and the like.
Therefore, the method has wide application prospect in 'repairing' the industrial effluent polluted by the heavy metal, particularly for the water body with the heavy metal content below 100mg/L, the metal ions are removed by adopting other methods, the treatment is difficult or the cost is high, and the heavy metal ions can be effectively removed by using easily obtained and cheap raw materials, namely waste (such as fermentation byproducts) of certain industries or organisms (such as algae) which exist in large quantities in the nature as biological adsorbents.
The prior art needs to be improved and developed.
Disclosure of Invention
The invention aims to provide a preparation and application method of a microbial inoculum for drainage basin hexavalent chromium pollution treatment.
The technical solution of the invention is as follows: a preparation method of a microbial inoculum for treating hexavalent chromium pollution in a drainage basin is disclosed, wherein the microbial inoculum is Streptomyces puniceus strain LYzhlm, and the preservation number of the microbial inoculum is CGMCC No.11773, and the preparation method is characterized by comprising the following steps:
step 1, preparing a fermentation raw material: preparing a fermentation raw material according to the mass percentage of the liquid molasses, the industrial glucose 1.0-5%, the industrial yeast powder 0.5%, the bran powder 3.0% (the grinding requirement is less than 100 meshes), the malt extract 1.0%, the dipotassium hydrogen phosphate 0.3%, the sodium dihydrogen phosphate 0.1%, the ammonium sulfate 0.5% and the magnesium sulfate 0.05%;
step 2, sterilization: putting the raw materials in the step 1 into a fermentation tank for sterilization, wherein the sterilization temperature is 121 ℃, and the sterilization time is 0.5 hour;
step 3, fermentation: fermenting the fermentor containing the fermentation feedstock after sterilization; the fermentation temperature is controlled to be 30-32 ℃, the fermentation time is 96 hours, and during fermentation, the ventilation gas: the liquid aeration ratio is 1:0.5 in 0-24 hours, 1:1 in 24-96 hours, the tank pressure of a fermentation tank is 0.08Mpa, and the fermentation raw materials are stirred all the time after being put into the fermentation tank, wherein the stirring speed is 200 rpm;
and 4, checking the total number of bacteria of the fermentation broth produced by fermentation, filling and packaging to obtain a finished product.
And 5, adding the LYzhlm microbial inoculum into the hexavalent chromium polluted water, wherein the volume ratio of the microbial inoculum to the polluted water is 0.5-3.0: 100(L/L), controlling the pH value of the polluted water added with the microbial inoculum to be 5-9, controlling the temperature to be 25-35 ℃, and controlling the stirring speed to be 80-140 r/min.
And 6, the weight-volume ratio of the polluted water is 2.0:100 g/ml.
And 7, adding the microbial inoculum to obtain the polluted water with the pH value of 7.0.
And step 8, adding the microbial inoculum, and then heating the polluted water to 30 ℃.
The invention has the beneficial effects that:
the LYzhlm fermentation liquor has a strong hexavalent chromium reduction effect. The effect evaluation in the laboratory shows that the LYzhlm strain has strong performance of removing hexavalent chromium, and can reduce hexavalent chromium ions with the concentration of 100 mg/L-1000 mg/L to 0 under the conditions of oxygen and medium temperature, and the reduction rate is 100%. 2 times of removal experiments are carried out on the industrial hexavalent chromium polluted water, the initial concentrations of hexavalent chromium in the polluted water before the experiments are 1790mg/L and 972mg/L respectively, and the concentrations of hexavalent chromium in the polluted water treated by the method are reduced to 0.08mg/L and 0.046mg/L, so that the discharge standard of hexavalent chromium is reached. Compared with the prior art, the method can treat the hexavalent chromium-containing wastewater with higher concentration, and has high reduction efficiency.
Drawings
FIG. 1 is a curve for removing hexavalent chromium ions from hexavalent chromium wastewater treated by LYzhlm.
Detailed Description
The invention discloses a Streptomyces punicus strain LYzhlm capable of efficiently removing hexavalent chromium pollution, which is preserved in the common microorganism center of China general microbiological culture Collection management Committee on 12.2.2015, wherein the preservation number is CGMCC No.11773, and the Streptomyces punicus strain LYzhlm is named. Address: the institute of microbiology of western-style academy of sciences, north-Chen, Chaozhou, Chaoyang.
The 16S rDNA sequence is the sequence of the sequence table SEQ NO. 1.
The streptomycete for efficiently removing hexavalent chromium pollution is applied to the aspect of removing hexavalent chromium pollution.
The LYzhlm strain is derived from industrial sludge, and is subjected to indoor domestication, separation, purification and screening. Culturing LYzhlm strain in LB culture medium, and making the cell form be filamentous; after culturing for 4 days, the color of the colony is grey white, and the colony is round and unsmooth. The intrabasal hyphae did not break, and the aerial hyphae generally developed well to form long spore filaments. The intrabasal hyphae have many branches and produce a purplish red water-soluble pigment. The spore silk shape was microscopically observed. The spores are oval and have smooth surfaces.
The most common valence states of chromium in soil are trivalent chromium and hexavalent chromium, and in natural environment, hexavalent chromium is easily reduced to trivalent chromium by microorganisms, while trivalent chromium is difficult to be oxidized to hexavalent chromium. Hexavalent chromium in soils is commonly found as chromate (CrO)4 2-) And dichromate (Cr)2O4 2-) The product has high activity and strong toxic action on organisms. Trivalent chromium is mainly Cr (H) in soil, compared with hexavalent chromium2O)6 3+、Cr(H2O)2+、CrO2+The fertilizer has the advantages of existing forms, easy adsorption by soil or generation of sediment, low toxic and harmful effects on organisms and relatively small influence on the environment. The Streptomyces purpureus (Streptomyces purpureus strain LYzhlm) obtained by screening and domesticating changes the chemical form of heavy metal, fixes the heavy metal, reduces the bioavailability and reduces the harm of the heavy metal. Compared with physical and chemical repair technologies, the LYzhlm repair technology provides an efficient strain for treating the high pollution of hexavalent chromium due to the advantages of safety, non-destructiveness and economy.
The LYzhlm strain is amplified by PCR method using bacterial 16S rDNA universal primer. 16S rDNA partial fragment, PCR product sequencing to obtain the fragment sequence, sequencing the 16S rDNA (length 1.4kbp) of LYzhlm strain as shown in sequence table SEQ No. 1.
The homology between the 16S rDNA sequence of LYzhlm strain and Streptomyces aureofaciens is highest and reaches 99% by performing gene homology and similarity analysis by using BLAST tool on the sequence table SEQ NO.1 and performing BLAST search (see Table 1)
TABLE 1 BLAST search results Table
According to the BLAST analysis results, the strain belongs to the following biological taxonomy information:
domain：Bacteria；
phylum：Actinobacteria；
class：Streptomycetales；
order：Streptomycetaceae；
family：Streptomyces；
Genus：Streptomyces puniceus。
thus, the new strain was identified as Streptomyces purpureus (Streptomyces puniceus strain) and named Streptomyces purpureus (Streptomyces puniceus strain LYzhlm).
The preparation method of the strain comprises the following steps:
step 1, preparing a fermentation raw material: preparing a fermentation raw material according to the mass percentage of the liquid molasses, the industrial glucose 1.0%, the industrial yeast powder 0.5%, the bran powder 3.0% (the grinding requirement is less than 100 meshes), the malt extract 1.0%, the dipotassium phosphate 0.3%, the sodium dihydrogen phosphate 0.1%, the ammonium sulfate 0.5% and the magnesium sulfate 0.05%;
step 2, sterilization: putting the raw materials in the step 1 into a fermentation tank for sterilization, wherein the sterilization temperature is 121 ℃, and the sterilization time is 0.5 hour;
step 3, fermentation: fermenting the fermentor containing the fermentation feedstock after sterilization; the fermentation temperature is controlled to be 30-32 ℃, the fermentation time is 96 hours, and during fermentation, the ventilation gas: the liquid aeration ratio is 1:0.5 in 0-24 hours, 1:1 in 24-96 hours, the tank pressure of a fermentation tank is 0.08Mpa, and the fermentation raw materials are stirred all the time after being put into the fermentation tank, wherein the stirring speed is 200 rpm;
and 4, checking the total number of bacteria of the fermentation broth produced by fermentation, filling and packaging to obtain a finished product.
And 5, adding the LYzhlm microbial inoculum into the hexavalent chromium polluted water, wherein the volume ratio of the microbial inoculum to the polluted water is 0.5-3.0: 100(L/L), controlling the pH value of the polluted water added with the microbial inoculum to be 5-9, controlling the temperature to be 25-35 ℃, and controlling the stirring speed to be 80-140 r/min.
And 6, the weight-volume ratio of the polluted water is 2.0:100 g/ml.
And 7, adding the microbial inoculum to obtain the polluted water with the pH value of 7.0.
And step 8, adding the microbial inoculum, and then heating the polluted water to 30 ℃.
Example 1 basis for detection
The method for measuring hexavalent chromium in sewage comprises the following steps:
dihydrazine diphenylcarbonate spectrophotometry for determining hexavalent chromium in water quality GB7467-1987
The method for measuring the total chromium in the sewage comprises the following steps:
method for measuring total chromium in water by oxidizing potassium permanganate and dihydrazide diphenyl carbonate spectrophotometry GB7466-1987
Example 2 the specific steps of the product of the invention for treating high concentration hexavalent chromium heavy metal contaminated water are as follows:
the product produced by the method is added into 1 of hexavalent chromium polluted water, and the detected concentration of the hexavalent chromium contained in the 1 of the polluted water is 1790 mg/L. The volume ratio of the product to the polluted water is 2.5:100(L/L), the pH value of the polluted water is adjusted to be 7.0, the temperature of the polluted water is controlled to be 30 ℃, the stirring speed is 100r/min, samples are taken at the treatment interval of 24 hours, and the concentration of total chromium and hexavalent chromium is measured. The sampling was stopped after the Cr (VI) concentration in the medium was below the detection limit.
Example 3 the specific steps of the product of the invention for treating hexavalent chromium heavy metal polluted water with medium difficulty are as follows:
the product produced by the method is added into 2 of the hexavalent chromium polluted water, and the detected hexavalent chromium concentration of the polluted water 2 is 972 mg/L. The volume ratio of the product to the polluted water is 2.0:100(L/L), the pH value of the polluted water is adjusted to be 7.0, the temperature of the polluted water is controlled to be 30 ℃, the stirring speed is 100r/min, samples are taken at the treatment interval of 24 hours, and the concentration of total chromium and hexavalent chromium is measured. The sampling was stopped after the Cr (VI) concentration in the medium was below the detection limit.
The data obtained for the removal of hexavalent chromium contaminated water by the process described in examples 2 and 3 is shown in table 2:
TABLE 2 result table for removing hexavalent chromium from waste water
As can be seen from Table 2, the method of the present invention has a significant improvement in the removal of hexavalent chromium from contaminated water by conventional microbiological methods.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The above detailed description is specific to possible embodiments of the present invention, and the embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention are intended to be included within the scope of the present invention.
Sequence listing
<110> land area ecological engineering Co., Ltd
<120> streptomycete for efficiently removing hexavalent chromium pollution and application thereof
<160>1
<210> 1
<211> 1431
<212> DNA
<213> Artificial sequence
<400> 1
CTTACCTAACCGCGCTTACCATGCAGTCGACGATGAACCGCTTCGGTGGTGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTTCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATAATACTCCTGCCTGCATGGGTGGGGGTTGAAAGCTCCGGCGGTGAAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGGGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGCAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATATACCGGAAAGCATCAGAGATGGTGCCCCCCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCTTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATGCCGTGAGGCGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTGTGGGAGGAGCTTCGAAGTGACTTCTTGGGGTC
Claims (5)
1. A preparation method of a microbial inoculum for treating hexavalent chromium pollution in a drainage basin comprises the following bacterial strains Streptomyces puniceus strain LYzhlm, the preservation number of which is CGMCC No.11773, and the 16S rDNA sequence is the sequence of a sequence table SEQ No.1, and is characterized by comprising the following steps:
step 1, preparing a fermentation raw material: preparing a fermentation raw material according to the mass percentage of the liquid molasses and the water, wherein the liquid molasses is 5.0 percent, the industrial glucose is 1-5 percent, the industrial yeast powder is 0.5 percent, the bran powder is 3.0 percent, the crushing requirement is less than 100 meshes, the malt extract is 1.0 percent, the dipotassium hydrogen phosphate is 0.3 percent, the sodium dihydrogen phosphate is 0.1 percent, the ammonium sulfate is 0.5 percent, and the magnesium sulfate is 0.05 percent;
step 2, sterilization: putting the raw materials in the step 1 into a fermentation tank for sterilization, wherein the sterilization temperature is 121 ℃, and the sterilization time is 0.5 hour;
step 3, fermentation: fermenting the fermentor containing the fermentation feedstock after sterilization; the fermentation temperature is controlled to be 30-32 ℃, the fermentation time is 96 hours, and during fermentation, the ventilation gas: the liquid aeration ratio is 1:0.5 in 0-24 hours, 1:1 in 24-96 hours, the tank pressure of a fermentation tank is 0.08Mpa, and the fermentation raw materials are stirred all the time after being put into the fermentation tank, wherein the stirring speed is 200 rpm;
and 4, checking the total number of bacteria of the fermentation broth produced by fermentation, filling and packaging to obtain a finished product.
2. The use method of the microbial inoculum prepared by the method for preparing the microbial inoculum for treating the hexavalent chromium pollution of the drainage basin according to claim 1, is characterized in that: the LYzhlm microbial inoculum is added into hexavalent chromium polluted water, the volume ratio of the microbial inoculum to the polluted water is 0.5L-3.0L: 100L, the pH value of the polluted water added with the microbial inoculum is controlled to be 5-9, the temperature is controlled to be 25-35 ℃, and the stirring speed is 80-140 r/min.
3. The use method of the microbial inoculum prepared by the microbial inoculum preparation method for treating the hexavalent chromium pollution of the drainage basin according to claim 2, is characterized in that: the weight-volume ratio of the lyszhlm bacterial agent to the polluted water is 2.0:100 g/ml.
4. The use method of the microbial inoculum prepared by the microbial inoculum preparation method for treating the hexavalent chromium pollution of the drainage basin according to claim 2, is characterized in that: the pH value of the polluted water added with the microbial inoculum is 7.0.
5. The use method of the microbial inoculum prepared by the microbial inoculum preparation method for treating the hexavalent chromium pollution of the drainage basin according to claim 2, is characterized in that: the temperature of the polluted water after the microbial inoculum is added is 30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610078160.7A CN106367363B (en) | 2016-02-03 | 2016-02-03 | Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610078160.7A CN106367363B (en) | 2016-02-03 | 2016-02-03 | Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106367363A CN106367363A (en) | 2017-02-01 |
| CN106367363B true CN106367363B (en) | 2020-01-07 |
Family
ID=57880212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610078160.7A Expired - Fee Related CN106367363B (en) | 2016-02-03 | 2016-02-03 | Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106367363B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107827382A (en) * | 2017-11-16 | 2018-03-23 | 广东弘朝科技有限公司 | A kind of hexavalent chrome reduction agent and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205384A (en) * | 2013-04-17 | 2013-07-17 | 广州利万世环保科技有限公司 | Soil heavy metal hexavalent chromium biological degrading agent and production method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611250A (en) * | 2014-11-24 | 2015-05-13 | 苏州嘉禧萝生物科技有限公司 | Chromium polluted soil composite microbial repairing agent, preparation method and application thereof |
| CN104498042A (en) * | 2014-11-24 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Microorganism repairing agent and applications thereof in chromium polluted soil restoration |
-
2016
- 2016-02-03 CN CN201610078160.7A patent/CN106367363B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205384A (en) * | 2013-04-17 | 2013-07-17 | 广州利万世环保科技有限公司 | Soil heavy metal hexavalent chromium biological degrading agent and production method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Z. Chen et al..Thioredoxin is involved in hexavalent chromium reduction in Streptomyces violaceoruber strain LZ-26-1 isolated from the Lanzhou reaches of the Yellow River.《International Biodeterioration & Biodegradation》.2014,第94卷第146-151页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106367363A (en) | 2017-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mishra et al. | Reduction of hexavalent chromium by Microbacterium paraoxydans isolated from tannery wastewater and characterization of its reduced products | |
| Afzal et al. | Assessment of heavy metal tolerance and biosorptive potential of Klebsiella variicola isolated from industrial effluents | |
| Sharma et al. | Detoxification and accumulation of chromium from tannery effluent and spent chrome effluent by Paecilomyces lilacinus fungi | |
| Bakthavatsalam | A comparative study on growth and degradation behavior of C. pyrenoidosa on synthetic phenol and phenolic wastewater of a coal gasification plant | |
| Shakoori et al. | Hexavalent chromium reduction by a dichromate-resistant gram-positive bacterium isolated from effluents of tanneries | |
| Venkatesan et al. | Reduction of hexavalent chromium to trivalent chromium from tannery effluent using bacterial biomass. | |
| Saranraj et al. | Microbial bioremediation of chromium in tannery effluent: a review | |
| Gupta Mahendra et al. | Bioremediation of heavy metal polluted environment using resistant bacteria | |
| Morales-Barrera et al. | Isolation, identification and characterization of a Hypocrea tawa strain with high Cr (VI) reduction potential | |
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| Jafari et al. | Biodecolorization of textile azo dyes by isolated yeast from activated sludge: Issatchenkia orientalis JKS6 | |
| Zhao et al. | Removal of hexavalent chromium in tannery wastewater by Bacillus cereus | |
| CN100552019C (en) | One strain denitrogen paracoccus and cultural method thereof and application | |
| Queiroz et al. | Rich growth medium promotes an increased on Mn (II) removal and manganese oxide production by Serratia marcescens strains isolates from wastewater | |
| Bahafid et al. | Removal mechanism of hexavalent chromium by a novel strain of Pichia anomala isolated from industrial effluents of Fez (Morocco) | |
| Purwanti et al. | Identification of acid and aluminium resistant bacteria isolated from aluminium recycling area | |
| CN109337825B (en) | A strain of Paecilomyces beijingensis LYZ7 and its application | |
| Aravindhan et al. | Biological removal of carcinogenic chromium (VI) using mixed Pseudomonas strains | |
| CN116042450A (en) | Equiella and application thereof for efficiently degrading anilines | |
| CN106367363B (en) | Preparation and use methods of microbial inoculum for drainage basin hexavalent chromium pollution treatment | |
| Saranraj et al. | Bioadsorption of chromium resistant Enterococcus casseliflavus isolated from tannery effluents | |
| CN106929448A (en) | One plant of acinetobacter calcoaceticus CZ1701 bacterial strain and application thereof of degraded different shape nitrogen | |
| CN106635855A (en) | Microbacterium kitamiense and culture application thereof | |
| CN106367362B (en) | It is a kind of to remove hexavalent chromium polluted streptomycete and its application | |
| Pinki et al. | Microbial reduction and detoxification of chromium from tannery effluent by natural inhabitants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200107 Termination date: 20220203 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |