CN106490218A - A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium - Google Patents
A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium Download PDFInfo
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Abstract
The method that a kind of black tea of utilization secondary fermentation of the present invention prepares jasmine tea golden flower bacterium, using the golden flower bacterium carried during secondary fermentation black tea, golden flower bacterium is individually taken out, place it in culture dish, after fully growing 34 days under normal temperature, then golden flower bacterium is placed on Amplification Culture in culture tank, cultivation temperature is controlled at 28 35 DEG C, cultivate 18 24 hours, obtain the golden flower bacterium after Amplification Culture and be diluted, obtain the golden flower bacterium dilution after Amplification Culture, then the golden flower bacterium dilution after will be enlarged by cultivating is sprayed on jasmine tea, then the indoor fermentation of enclosed fermentation for the jasmine tea for being sprayed with golden flower bacterium being positioned over 35 40 DEG C 15 18 days, golden flower bacterium is allowed to grow in jasmine tea top fermentation, finally give jasmine tea golden flower bacterium.The present invention is the preparation method of improved jasmine tea golden flower bacterium on the basis of CN 201310443574.1 so that the jasmine tea golden flower bacterium sweetness that makes is tasty and refreshing, and gives off a strong fragrance, and is liked by the vast person of having tea.
Description
Technical field
The invention belongs to health protection tea development field, and in particular to a kind of black tea of utilization secondary fermentation prepares jasmine tea gold
The method of flower bacterium.
Background technology
Due to black tea kneading, pile fermentation, rub up fermentation again when be that high-volume is stacked and carried out, much the tealeaves on side is just
Be not as uniform as the tea leaf fermentation at center, being also possible to of having catches miscellaneous bacteria, causes product heterogeneity, and had is also defective in quality.And
And due to displaying during fermentable be subject to display locality climatic effect than larger, assembly time, the taste of tealeaves
Relevant with the weather of different regions, the material one time fermentation allowed by natural conditions in black tea makes the quality of product ensure,
If catching miscellaneous bacteria, the tealeaves possibility mouthfeel and fragrance that consumer has bought is bad, thus cannot obtain the homogeneous product of quality.
Therefore, the applicant has applied for the black tea of secondary fermentation and preparation method and apparatus thereof on 26th in September in 2013
Chinese patent, application number：201310443574.1 disclosures of CN (bulletin) number：CN103493916A, there is provided secondary fermentation black
Tea and preparation method and apparatus thereof, processing step are as follows：（1）The fresh tea leaf that will be plucked, completes, then spreading for cooling, and tealeaves is aqueous
Amount is kneaded, is allowed to be curled into bar in 50-60, after kneading end, deblocks, and drying makes tea moisture content in 88-90%, so
Pile fermentation, dismisses, sieves, make one time fermentation black tea afterwards；（2）The black tea tealeaves that one time fermentation is crossed is placed on pallet and bracket,
Bracket is placed in fermentation interior, opens steam, steam is diffused between tealeaves, and at 50-65 DEG C, the time is that 5-15 is little to temperature control
When, then stopping supplying steam, allow tealeaves in the fermentation interior fermentation that closes 3～7 days, after fermentation is completed, ventilation is naturally cold
But and dry, obtain the black tea product of secondary fermentation.Through the black tea clean hygiene of secondary fermentation, quality is superior, dark brown uniform,
Better quality, with fragrant after water cannon system, aromatic good to eat, there are a lot of health-care effects to human body, be the treasure in full fermentation tea.
In the black tea of above-mentioned secondary fermentation, secondary fermentation process passes first into steam so as to which temperature reaches 50-65 DEG C, will
Miscellaneous bacteria kill, then adjust tealeaves temperature and humidity, to during tealeaves one time fermentation per se with actinomyces breeding suitable
Condition, and fully breed, after secondary fermentation, just visible in tea have many golden yellow " golden flower ", and this is the bacterium of beneficial qualities
Kind, it can secreting amylase and oxidizing ferment, the Starch Conversion that can be catalyzed in tealeaves is monose, catalysis polyphenol compound oxidation,
Make tealeaves soup look become reddish brown, eliminate thick green grass or young crops taste.The natural tealeaves compared with one time fermentation contain more amino acids needed by human and
Vitamin, contained lipase are higher than other teas, therefore secondary fermentation black tea has, and higher decomposition is greasy, reduce human body
Class fatty compound, cholesterol, effect of triglyceride, drink for a long time can be reposed with stomach invigorating, body-building and weight-reducing, with people give birth to
The flat raising of running water, the black tea of secondary fermentation are more liked by consumer.
Jasmine, another name：Jasmine, Latin literary fame：Jasminum sambac (L.) Ait, Oleaceae, gelsemium upright or
Climbing shrub, up to 3 meters.Sprig cylinder or slightly flattening shape, sometimes hollow, dredge by pubescence.Leaf opposite, Dan Ye, blade papery,
Circular, oval, ovate-elliptic or obovate, two terminal circle is blunt, base portion micro-heart sometimes, above slightly concave enter or recessed,
Protruding below, in two sides Chang Mingxian, microprotrusion, in addition to often having tuft between following arteries and veins, remaining is without hair for thready pulse；Sliver Long Circle is extremely
Subcircular, tip circle or blunt.Really spherical, in atropurpureus.The month at florescence 5-8, the fruiting period 7-9 month.The Hua Jixiang of jasmine, is famous flower
Tea raw material and important flavor of raw material；Flower, leaf is medicinal controls red eye, swell pain, and has relieving cough and reducing sputum effect.
Jasmine tea is the high-grade tealeaves with Jasmine scenting, is one of ten big jasmine tea of China, is to select from the Sheng in spring
The Jasmine for opening and tealeaves mix into jasmine tea together, and the fragrance with spring flower has the pure and fresh gas of tealeaves again, are that mouthfeel is fragrant
The sweet jasmine tea that is welcome by everybody very much.Often drink jasmine tea for women not only can beautifying face and moistering lotion, net pale skin can also
Enough the tail in youth is kept in opposing aging here.Human body stomach can also be dredged can to arrange stool, pleasant cephalocathartic, reduce blood pressure and blood fat,
Also there is huge effect and effect of opposing bacterium and viral therapy cancer.
At present, be used for brewing the tea product of the relevant Jasmine that drinks on market, have a large amount in variety, have jasmine flower green tea,
Jasmine black tea etc., but the report about jasmine tea golden flower bacterium do not have, and due to the difference of the manufacture craft of all kinds of tea, makes into
The quality that samples tea also generates larger difference, has the jasmine tea product that a lot of mouthfeels are more preferable, fragrance is richer to need people
Research finds.
Applicant location is " township of Chinese jasmine " ----Heng County, has also applied a series of with regard to the special of Jasmine
Profit.Such as Application No. 200710066205.X, the Chinese patent of the processing method of entitled jasmine iron goddess tea；Application
Number be 201410350821.8, the Chinese patent of the preparation method of entitled jasmine Aglaia odorata liu bao tea；Application No.
201410350822.2, the Chinese patent of the preparation method of entitled secondary fermentation Jasmine Mulberry-leaf Tea.Above studying
On the basis of, it is found by the applicant that the golden flower bacterium contained in the black tea of secondary fermentation can be applied on jasmine tea, Liang Zhexiang
Fusion, after the sweaty aroma of jasmine tea is brought into play with causing to the greatest extent, also makes jasmine tea add health care.
And golden flower bacterium can produce a kind of enzyme, promote the protein in tealeaves, Tea Polyphenols changes into special essence russet
Magnificent material, this material sweetness are tasty and refreshing, can improve the equilibrium between yin and yang of people, strengthen function of human body, keep out disease invasion.Golden flower bacterium is
Nonnutritive, its effect is to produce a kind of enzyme to help the protein in tealeaves, and starch, changing into for polyphenols are right
The beneficial material of human body.Showing as mouthfeel in the more qualities of golden flower bacterium, more to sweeten cunning sweet refreshing, and soup look is redder gorgeous bright, in Fu bricks
It is better to produce several years difficult " the heap tastes " for dissipating in thick green grass or young crops taste being eliminated rapidly in tea and being fermented, and to digestive system and the improvement of sleep
Effect is significant.
Content of the invention
It is an object of the invention to provide one kind gives off a strong fragrance, the system for having the jasmine tea golden flower bacterium product of health function
Preparation Method, is the preparation method of improved jasmine tea golden flower bacterium on the basis of CN 201310443574.1 so that makes and
Jasmine tea golden flower bacterium rich in nutrition content, and give off a strong fragrance, liked by the vast person of having tea.
A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium, comprises the steps：
（1）One time fermentation, the mixing tealeaves that will be plucked from tree are rejected yellow leaf, branch stalk and debris grass, are completed, and pot temperature is rested in
In the range of 220 DEG C～280 DEG C, fixation time 7～10 minutes, then spreading for cooling, tealeaves wet weight content are used in 50-60
Kneading machine is kneaded, and the degree of kneading will accomplish to be curled into bar, and uniformity, the incipient stage that kneads must be with gently rubbing, gradually later
Strengthen, knead minute total time 7-10, after kneading end, deblock, drying makes tea moisture content in 88-90%, then wet
Heap, pile fermentation dry later tealeaves, first dismiss, sieve, and stepping makes one time fermentation black tea；
（2）The black tea tealeaves that crosses through one time fermentation is placed in pallet, pallet is placed on bracket, and bracket is placed in fermenting cellar
Interior, fermentation interior is provided with steam pipe, closes the door of fermenting cellar, opens steam, steam is diffused between tealeaves, and temperature control exists
50-65 DEG C, the time is 5-15 hours, then stops supplying steam, allows tealeaves in the fermentation interior fermentation 3～7 days of closing, no
Need to add any bacterial classification, by during tealeaves one time fermentation per se with golden flower bacterium oneself breeding, fermentation temperature maintains 40～
55 DEG C, if too high or too low for temperature, lead to steam or Ventilation Control, after fermentation is completed, open the door of fermenting cellar, divulge information,
Allow the temperature natural cooling of tealeaves and dried for a week, obtain the black tea product of secondary fermentation；
（3）Take step（2）The black tea product of the secondary fermentation for obtaining, according to black tea product:The part by weight of water is 100:5, will
Black tea product after normal temperature bottom fermentation culture 5-6 days, is waited after growing golden flower bacterium, golden flower bacterium is individually taken with water mixing and stirring
Go out, place it in the culture dish with sterilized water, after fully growing 3-4 days under normal temperature, golden flower bacterium is placed on training then
Amplification Culture in foster tank, cultivation temperature are controlled at 28-35 DEG C, are cultivated 18-24 hours, are obtained the golden flower bacterium after Amplification Culture；Press
According to sterilized water:The part by weight of the golden flower bacterium after Amplification Culture is 5:1 will be enlarged by the golden flower bacterium after culture is diluted, and is expanded
Golden flower bacterium dilution after big culture, according still further to jasmine tea:The part by weight of the golden flower bacterium dilution after Amplification Culture is 8:1
Golden flower bacterium dilution after will be enlarged by cultivating is sprayed on jasmine tea, and the jasmine tea for being sprayed with golden flower bacterium is positioned over 35-
40 DEG C of enclosed fermentation interior fermentation 15-18 days, after jasmine tea grows golden flower bacterium, opens the door of fermenting cellar, divulges information,
Allow length to have the temperature natural cooling of the jasmine tea of golden flower bacterium, then length had the jasmine tea cake of press 5 minutes of golden flower bacterium, finally
Jasmine tea after by cake of press is positioned over drying room drying, and controls its water content less than 10%, is packed with linen-cotton or lotus leaves,
Obtain jasmine tea golden flower bacterium product.
Step（3）In culture medium by agar, dregs of beans, molasses, peptone, beef extract, KH2PO4With NaCl according to weight portion
Number compares 2:2:3:1:1:0.2:After 0.1 mixing, the water diluting of 100 times of weight of peptone is added to stir, adjusting pH value is
6.6-6.8.
Step（3）Described in jasmine tea include that jasmine green tea, jasmine white tea, jasmine Dragon Well tea, jasmine black tea, jasmine are general
Pu'er tea life tea, jasmine Pu'er cooked tea, jasmine green grass or young crops tea, jasmine yellow tea, jasmine Iron Guanyin, six fort of jasmine clovershrub and jasmine.
Preferably, step（3）Described in jasmine tea be jasmine white tea.
And add molasses in Amplification Culture base（The non crystallized containing sugar liquors that sugar refinery sugar industry is separated）Also have
Important function：（1）As the carbon source of Amplification Culture base, sucrose is substituted, reduce production cost；（2）It is more easy to containing a large amount of golden flower bacteriums
In the various monose, thick protein and the mineral matter that utilize, compared with sucrose nutrition more fully.Other culture based compounds can be from
To its title and effect, it is right that the present invention is combined the textbooks of chemical dictionary or golden flower bacterium culture using these compounds
The culture of golden flower bacterium is suitable for the most, particularly plus the molasses rich in nutrient content, is through much screening just last determination.
Contain creatine, kreatinin, polypeptide, amino acids, ucleotides, organic acid, mineral matter class in the middle of beef extract
And the water-soluble substances of vitamins.The product is widely used in bio-pharmaceuticals fermentation and the preparation of various culture mediums.Central
The Main Function that it rises is the subalimentation for supplementing peptone and other nitrogen sources, and general consumption is 0.3%～0.5%.Beef
Cream protein culture medium is a kind of most widely used and most common bacterium basal medium, is sometimes referred to as ordinary culture medium.
Due to the most basic nutriment required for breeding containing general bacterial growth in this culture medium, so microorganism can be made for
Growth and breeding is used.Basal medium contains beef extract, peptone and NaCl.Wherein beef extract provides carbon source, energy for microorganism
Source, phosphate and vitamin, peptone mainly provide nitrogen source and vitamin, and NaCl provides inorganic salts.Due to this culture medium
It is used for cultivating bacterium, its pH is adjusted to neutrality or alkalescence with diluted acid or diluted alkaline therefore, is beneficial to the growth and breeding of bacterium.
Agar, scientific name agar-agar, English name（agar）, also known as agar (agar-agar）, Hai Dongcai, agar, agar-agar, parmelia saxatilis
Glue, delicacy made from bird's nests essence, agel-agal, cold day, big dish slice, are one kind of natural plant gum, and the Eucheuma of conventional marine products, agar, fragrant plant mentioned in ancient texts etc. are made,
For colourless solid without solid shape, hot water is dissolved in.It is widely used in the food industry, is also commonly used for bacteria culture media.
The present invention optimizes the ratio of each nutrient content, while taking into account cost for the purpose of viable count highest.Generally require excellent
Change the content of carbon source nitrogen source ratio and trace element, and dregs of beans and molasses are lower-cost desirable feedstocks.
Compared with prior art, the positive effect of the present invention is：
1st, jasmine tea golden flower bacterium mouthfeel of the invention is more preferable, fragrance is richer and processing technology is simple, ensureing active ingredient not
There is while loss the feature of the strong fragrant fragrance of jasmine tea, the present invention to make jasmine tea and golden flower bacterium obtain diversification again
Exhibition, for the popularization of tea culture and developing for tea industry, has great significance and far-reaching influence.
2nd, the present invention is the preparation method of improved jasmine tea golden flower bacterium on the basis of CN 201310443574.1,
So that the jasmine tea golden flower bacterium sweetness that makes is tasty and refreshing, and give off a strong fragrance, liked by the vast person of having tea.
3rd, the present invention is placed on training using the golden flower bacterium carried during secondary fermentation black tea after golden flower bacterium is individually taken out
In foster ware, fully grow under normal temperature 3-4 days, golden flower bacterium is placed on Amplification Culture in culture tank then, cultivation temperature control exists
28-35 DEG C, 18-24 hours are cultivated, the golden flower bacterium after Amplification Culture is obtained and is diluted, obtain the golden flower after Amplification Culture
Bacterium dilution, the golden flower bacterium dilution after then will be enlarged by cultivating are sprayed on jasmine tea, will then be sprayed with the jasmine of golden flower bacterium
Jasmine jasmine tea is positioned over 35-40 DEG C of enclosed fermentation interior fermentation 15-18 days, allows golden flower bacterium to give birth in jasmine tea top fermentation
Long, finally give jasmine tea golden flower bacterium.The jasmine tea golden flower bacterium for obtaining is prepared by the method for the present invention, just visible in tea
There are many golden yellow " golden flower "（That is golden flower bacterium）, it can secreting amylase and oxidizing ferment, the Starch Conversion that can be catalyzed in tealeaves is
Monose, is catalyzed polyphenol compound oxidation, makes tealeaves soup look redder gorgeous bright, and mouthfeel more sweetens sliding sweet refreshing, the thick green grass or young crops taste of elimination, because
This jasmine tea golden flower bacterium possessing on the basis of jasmine tea gives off a strong fragrance, also use golden flower bacterium can produce a kind of special
Enzyme, promotes the protein in tealeaves, Tea Polyphenols to change into special essence substance russet, and this material sweetness is tasty and refreshing,
And the equilibrium between yin and yang of people can be improved, strengthen function of human body, keep out disease invasion, by the vast person of having tea and the crowd of emphasis health care
Person likes.
The health-care effect of jasmine tea golden flower bacterium
Golden flower（Bulk bacteria）：It is China and foreign countries microbiologist microorganism of the new discovery less than 30 years on earth, the past is only in thousand
Chanced on glossy ganoderma, black tea through separation identification compound there are about 500 kinds, wherein organic compound more than 450 kinds,
But result from a large number " bulk bacteria " in Fu tea exclusive in all tealeaves, be God to mankind's mystery " present ".
" golden flower " bacterium is a kind of extremely strong surging bacterial classification of inhibitory action, as long as it grows out, other miscellaneous bacterias are just suppressed
, therefore, golden flower bacterium is otherwise known as " overlord bacterium ".Relevant result of study confirms, jasmine tea golden flower bacterium effectively can suppress intestinal cancer,
Cancer of the stomach and the expansion of HCC.
There is the specific component with notable lowering blood-fat and reducing weight and function of polysaccharide in Fu-brick tea, and be found that exclusive in Fu-brick tea
Two kinds of new active materials --- Fu theine A and Fu theine B." golden flower " in Fu tea can effectively adjust human body new old generation
Thank, and have functions that stronger lipid-loweringing, step-down, adjust carbohydate metabolism.In addition, the tea polysaccharide compound in black tea is hypoglycemic
Main component.Tea polysaccharide compound is commonly referred to tea polysaccharide, is the mixture that a class constituent is complicated and changes greatly.To several
The result that the content of tea polysaccharide of kind of teas is determined shows, the content of tea polysaccharide highest of black tea, and its composition activity is also than other tea
Class is eager to excel, this is because in fermented tea, due to glycosidase, protease, hydrolase effect, and it is shorter to define relative length
Sugar chain and peptide chain reason, the longer peptide chain of short peptide chain is more easy to be absorbed, and biologically active is higher, and this may be exactly that fermented tea is outstanding
Which is one of the reason for black tea tea polysaccharide blood sugar decreasing effect is better than other teas.
Specific embodiment
Embodiment 1
A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium, comprises the steps：
（1）One time fermentation, the mixing tealeaves that will be plucked from tree are rejected yellow leaf, branch stalk and debris grass, are completed, and pot temperature is rested in
In the range of 220 DEG C～280 DEG C, fixation time 7～10 minutes, then spreading for cooling, tealeaves wet weight content are used in 50-60
Kneading machine is kneaded, and the degree of kneading will accomplish to be curled into bar, and uniformity, the incipient stage that kneads must be with gently rubbing, gradually later
Strengthen, knead minute total time 7-10, after kneading end, deblock, drying makes tea moisture content in 88-90%, then wet
Heap, pile fermentation dry later tealeaves, first dismiss, sieve, and stepping makes one time fermentation black tea；
（2）The black tea tealeaves of 500 kilograms crossed through one time fermentation is placed in pallet, pallet is placed on bracket, and bracket is put
Indoor in fermentation, fermentation interior is provided with steam pipe, closes the door of fermenting cellar, opens steam, steam is diffused between tealeaves, temperature
At 50-65 DEG C, the time is 5-15 hours for degree control, then stops supplying steam, allows tealeaves in the fermentation interior fermentation 3 of closing
～7 days, it is not necessary to add any bacterial classification, by during tealeaves one time fermentation per se with golden flower bacterium oneself breeding, fermentation temperature tie up
Hold at 40～55 DEG C, if too high or too low for temperature, lead to steam or Ventilation Control, after fermentation is completed, open fermenting cellar
Door, ventilation allow the temperature natural cooling of tealeaves and dried for a week, obtain the black tea product of secondary fermentation；
（3）Take step（2）The black tea product of the secondary fermentation for obtaining, according to black tea product:The part by weight of water is 100:5, will
Black tea product after normal temperature bottom fermentation culture 5-6 days, is waited after growing golden flower bacterium, golden flower bacterium is individually taken with water mixing and stirring
Go out, place it in the culture dish with sterilized water, after fully growing 3-4 days under normal temperature, golden flower bacterium is placed on training then
Amplification Culture in foster tank, cultivation temperature are controlled at 28 DEG C, are cultivated 24 hours, are obtained the golden flower bacterium after Amplification Culture；According to aseptic
Water:The part by weight of the golden flower bacterium after Amplification Culture is 5:1 will be enlarged by the golden flower bacterium after culture is diluted, and obtains Amplification Culture
Golden flower bacterium dilution afterwards, according still further to jasmine tea:The part by weight of the golden flower bacterium dilution after Amplification Culture is 8:1 will be enlarged by
Golden flower bacterium dilution after culture is sprayed on jasmine tea, and the jasmine tea for being sprayed with golden flower bacterium is positioned over 35 DEG C of closing
The fermentation interior fermentation of formula 18 days, after jasmine tea grows golden flower bacterium, opens the door of fermenting cellar, and ventilation allows length to have golden flower bacterium
Jasmine tea temperature natural cooling, then length had the jasmine tea cake of press 5 minutes of golden flower bacterium, finally by cake of press after jasmine
Jasmine jasmine tea is positioned over drying room drying, and controls its water content less than 10%, is packed with linen-cotton or lotus leaves, obtains jasmine tea
Golden flower bacterium product.
Step（3）In culture medium by agar, dregs of beans, molasses, peptone, beef extract, KH2PO4With NaCl according to weight portion
Number compares 2:2:3:1:1:0.2:After 0.1 mixing, the water diluting of 100 times of weight of peptone is added to stir, adjusting pH value is
6.6-6.8.
Step（3）Described in jasmine tea be jasmine green tea.
Embodiment 2
A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium, comprises the steps：
（1）One time fermentation, the mixing tealeaves that will be plucked from tree are rejected yellow leaf, branch stalk and debris grass, are completed, and pot temperature is rested in
In the range of 220 DEG C～280 DEG C, fixation time 7～10 minutes, then spreading for cooling, tealeaves wet weight content are used in 50-60
Kneading machine is kneaded, and the degree of kneading will accomplish to be curled into bar, and uniformity, the incipient stage that kneads must be with gently rubbing, gradually later
Strengthen, knead minute total time 7-10, after kneading end, deblock, drying makes tea moisture content in 88-90%, then wet
Heap, pile fermentation dry later tealeaves, first dismiss, sieve, and stepping makes one time fermentation black tea；
（2）The black tea tealeaves of 500 kilograms crossed through one time fermentation is placed in pallet, pallet is placed on bracket, and bracket is put
Indoor in fermentation, fermentation interior is provided with steam pipe, closes the door of fermenting cellar, opens steam, steam is diffused between tealeaves, temperature
At 50-65 DEG C, the time is 5-15 hours for degree control, then stops supplying steam, allows tealeaves in the fermentation interior fermentation 3 of closing
～7 days, it is not necessary to add any bacterial classification, by during tealeaves one time fermentation per se with golden flower bacterium oneself breeding, fermentation temperature tie up
Hold at 40～55 DEG C, if too high or too low for temperature, lead to steam or Ventilation Control, after fermentation is completed, open fermenting cellar
Door, ventilation allow the temperature natural cooling of tealeaves and dried for a week, obtain the black tea product of secondary fermentation；
（3）Take step（2）The black tea product of the secondary fermentation for obtaining, according to black tea product:The part by weight of water is 100:5, will
Black tea product after normal temperature bottom fermentation culture 5-6 days, is waited after growing golden flower bacterium, golden flower bacterium is individually taken with water mixing and stirring
Go out, place it in the culture dish with sterilized water, after fully growing 3-4 days under normal temperature, golden flower bacterium is placed on training then
Amplification Culture in foster tank, cultivation temperature are controlled at 32 DEG C, are cultivated 22 hours, are obtained the golden flower bacterium after Amplification Culture；According to aseptic
Water:The part by weight of the golden flower bacterium after Amplification Culture is 5:1 will be enlarged by the golden flower bacterium after culture is diluted, and obtains Amplification Culture
Golden flower bacterium dilution afterwards, according still further to jasmine tea:The part by weight of the golden flower bacterium dilution after Amplification Culture is 8:1 will be enlarged by
Golden flower bacterium dilution after culture is sprayed on jasmine tea, and the jasmine tea for being sprayed with golden flower bacterium is positioned over 38 DEG C of closing
The fermentation interior fermentation of formula 16 days, after jasmine tea grows golden flower bacterium, opens the door of fermenting cellar, and ventilation allows length to have golden flower bacterium
Jasmine tea temperature natural cooling, then length had the jasmine tea cake of press 5 minutes of golden flower bacterium, finally by cake of press after jasmine
Jasmine jasmine tea is positioned over drying room drying, and controls its water content less than 10%, is packed with linen-cotton or lotus leaves, obtains jasmine tea
Golden flower bacterium product.
Step（3）In culture medium by agar, dregs of beans, molasses, peptone, beef extract, KH2PO4With NaCl according to weight portion
Number compares 2:2:3:1:1:0.2:After 0.1 mixing, the water diluting of 100 times of weight of peptone is added to stir, adjusting pH value is
6.6-6.8.
Step（3）Described in jasmine tea be jasmine white tea.
Embodiment 3
A kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium, comprises the steps：
（1）One time fermentation, the mixing tealeaves that will be plucked from tree are rejected yellow leaf, branch stalk and debris grass, are completed, and pot temperature is rested in
In the range of 220 DEG C～280 DEG C, fixation time 7～10 minutes, then spreading for cooling, tealeaves wet weight content are used in 50-60
Kneading machine is kneaded, and the degree of kneading will accomplish to be curled into bar, and uniformity, the incipient stage that kneads must be with gently rubbing, gradually later
Strengthen, knead minute total time 7-10, after kneading end, deblock, drying makes tea moisture content in 88-90%, then wet
Heap, pile fermentation dry later tealeaves, first dismiss, sieve, and stepping makes one time fermentation black tea；
（2）The black tea tealeaves that crosses through one time fermentation is placed in pallet, pallet is placed on bracket, and bracket is placed in fermenting cellar
Interior, fermentation interior is provided with steam pipe, closes the door of fermenting cellar, opens steam, steam is diffused between tealeaves, and temperature control exists
50-65 DEG C, the time is 5-15 hours, then stops supplying steam, allows tealeaves in the fermentation interior fermentation 3～7 days of closing, no
Need to add any bacterial classification, by during tealeaves one time fermentation per se with golden flower bacterium oneself breeding, fermentation temperature maintains 40～
55 DEG C, if too high or too low for temperature, lead to steam or Ventilation Control, after fermentation is completed, open the door of fermenting cellar, divulge information,
Allow the temperature natural cooling of tealeaves and dried for a week, obtain the black tea product of secondary fermentation；
（3）Take step（2）The black tea product of the secondary fermentation for obtaining, according to black tea product:The part by weight of water is 100:5, will
Black tea product after normal temperature bottom fermentation culture 5-6 days, is waited after growing golden flower bacterium, golden flower bacterium is individually taken with water mixing and stirring
Go out, place it in the culture dish with sterilized water, after fully growing 3-4 days under normal temperature, golden flower bacterium is placed on training then
Amplification Culture in foster tank, cultivation temperature are controlled at 35 DEG C, are cultivated 18 hours, are obtained the golden flower bacterium after Amplification Culture；According to aseptic
Water:The part by weight of the golden flower bacterium after Amplification Culture is 5:1 will be enlarged by the golden flower bacterium after culture is diluted, and obtains Amplification Culture
Golden flower bacterium dilution afterwards, according still further to jasmine tea:The part by weight of the golden flower bacterium dilution after Amplification Culture is 8:1 will be enlarged by
Golden flower bacterium dilution after culture is sprayed on jasmine tea, and the jasmine tea for being sprayed with golden flower bacterium is positioned over 40 DEG C of closing
The fermentation interior fermentation of formula 15 days, after jasmine tea grows golden flower bacterium, opens the door of fermenting cellar, and ventilation allows length to have golden flower bacterium
Jasmine tea temperature natural cooling, then length had the jasmine tea cake of press 5 minutes of golden flower bacterium, finally by cake of press after jasmine
Jasmine jasmine tea is positioned over drying room drying, and controls its water content less than 10%, is packed with linen-cotton or lotus leaves, obtains jasmine tea
Golden flower bacterium product.
Step（3）In culture medium by agar, dregs of beans, molasses, peptone, beef extract, KH2PO4With NaCl according to weight portion
Number compares 2:2:3:1:1:0.2:After 0.1 mixing, the water diluting of 100 times of weight of peptone is added to stir, adjusting pH value is
6.6-6.8.
Step（3）Described in jasmine tea be jasmine Dragon Well tea.
Embodiment 4
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 1, but step（3）Described in
Jasmine tea be jasmine black tea.
Embodiment 5
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 1, but step（3）Described in
Jasmine tea be jasmine Pu'er raw tea.
Embodiment 6
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 1, but step（3）Described in
Jasmine tea be jasmine Pu'er cooked tea.
Embodiment 7
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 2, but step（3）Described in
Jasmine tea be jasmine green grass or young crops tea.
Embodiment 8
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 2, but step（3）Described in
Jasmine tea be jasmine yellow tea.
Embodiment 9
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 3, but step（3）Described in
Jasmine tea be jasmine Iron Guanyin.
Embodiment 10
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 3, but step（3）Described in
Jasmine tea be jasmine clovershrub.
Embodiment 11
A kind of black tea of utilization secondary fermentation prepares the method for jasmine tea golden flower bacterium with embodiment 3, but step（3）Described in
Jasmine tea be six fort of jasmine.
Claims (4)
1. a kind of method that black tea of utilization secondary fermentation prepares jasmine tea golden flower bacterium, it is characterised in that：Comprise the steps：
（1）One time fermentation, the mixing tealeaves that will be plucked from tree are rejected yellow leaf, branch stalk and debris grass, are completed, and pot temperature is rested in
In the range of 220 DEG C～280 DEG C, fixation time 7～10 minutes, then spreading for cooling, tealeaves wet weight content are used in 50-60
Kneading machine is kneaded, and the degree of kneading will accomplish to be curled into bar, and uniformity, the incipient stage that kneads must be with gently rubbing, gradually later
Strengthen, knead minute total time 7-10, after kneading end, deblock, drying makes tea moisture content in 88-90%, then wet
Heap, pile fermentation dry later tealeaves, first dismiss, sieve, and stepping makes one time fermentation black tea；
（2）The black tea tealeaves that crosses through one time fermentation is placed in pallet, pallet is placed on bracket, and bracket is placed in fermenting cellar
Interior, fermentation interior is provided with steam pipe, closes the door of fermenting cellar, opens steam, steam is diffused between tealeaves, and temperature control exists
50-65 DEG C, the time is 5-15 hours, then stops supplying steam, allows tealeaves in the fermentation interior fermentation 3～7 days of closing, no
Need to add any bacterial classification, by during tealeaves one time fermentation per se with golden flower bacterium oneself breeding, fermentation temperature maintains 40～
55 DEG C, if too high or too low for temperature, lead to steam or Ventilation Control, after fermentation is completed, open the door of fermenting cellar, divulge information,
Allow the temperature natural cooling of tealeaves and dried for a week, obtain the black tea product of secondary fermentation；
（3）Take step（2）The black tea product of the secondary fermentation for obtaining, according to black tea product:The part by weight of water is 100:5, will
Black tea product after normal temperature bottom fermentation culture 5-6 days, is waited after growing golden flower bacterium, golden flower bacterium is individually taken with water mixing and stirring
Go out, place it in the culture dish with sterilized water, after fully growing 3-4 days under normal temperature, golden flower bacterium is placed on training then
Amplification Culture in foster tank, cultivation temperature are controlled at 28-35 DEG C, are cultivated 18-24 hours, are obtained the golden flower bacterium after Amplification Culture；Press
According to sterilized water:The part by weight of the golden flower bacterium after Amplification Culture is 5:1 will be enlarged by the golden flower bacterium after culture is diluted, and is expanded
Golden flower bacterium dilution after big culture, according still further to jasmine tea:The part by weight of the golden flower bacterium dilution after Amplification Culture is 8:1
Golden flower bacterium dilution after will be enlarged by cultivating is sprayed on jasmine tea, and the jasmine tea for being sprayed with golden flower bacterium is positioned over 35-
40 DEG C of enclosed fermentation interior fermentation 15-18 days, after jasmine tea grows golden flower bacterium, opens the door of fermenting cellar, divulges information,
Allow length to have the temperature natural cooling of the jasmine tea of golden flower bacterium, then length had the jasmine tea cake of press 5 minutes of golden flower bacterium, finally
Jasmine tea after by cake of press is positioned over drying room drying, and controls its water content less than 10%, is packed with linen-cotton or lotus leaves,
Obtain jasmine tea golden flower bacterium product.
2. the method that the black tea of utilization secondary fermentation according to claim 1 prepares jasmine tea golden flower bacterium, its feature exist
In：Step（3）In culture medium by agar, dregs of beans, molasses, peptone, beef extract, KH2PO4With NaCl according to ratio of weight and number
2:2:3:1:1:0.2:After 0.1 mixing, the water diluting of 100 times of weight of peptone is added to stir, regulations pH value is 6.6-
6.8.
3. the method that the black tea of utilization secondary fermentation according to claim 1 prepares jasmine tea golden flower bacterium, its feature exist
In：Step（3）Described in jasmine tea include jasmine green tea, jasmine white tea, jasmine Dragon Well tea, jasmine black tea, jasmine Pu'er life
Tea, jasmine Pu'er cooked tea, jasmine green grass or young crops tea, jasmine yellow tea, jasmine Iron Guanyin, six fort of jasmine clovershrub and jasmine.
4. the method that the black tea of utilization secondary fermentation according to claim 3 prepares jasmine tea golden flower bacterium, its feature exist
In：Step（3）Described in jasmine tea be jasmine white tea.
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| CN107080008A (en) * | 2017-06-21 | 2017-08-22 | 云南农业大学 | A kind of natural pile fermentation of golden flower Pu'er cooked teas loose tea is grown dim method |
| CN107114500A (en) * | 2017-06-21 | 2017-09-01 | 云南农业大学 | A kind of the natural of Pu'er tea compressed tea is aged method of growing dim |
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| CN108029799A (en) * | 2017-11-22 | 2018-05-15 | 广西大学 | A kind of jasmine tea preparation method of bulk bacteria floating |
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| CN108967584A (en) * | 2017-06-01 | 2018-12-11 | 勐海茶业有限责任公司 | The method for preparing fermented tea, the fermented tea prepared with this method and its application |
| CN107080008A (en) * | 2017-06-21 | 2017-08-22 | 云南农业大学 | A kind of natural pile fermentation of golden flower Pu'er cooked teas loose tea is grown dim method |
| CN107114500A (en) * | 2017-06-21 | 2017-09-01 | 云南农业大学 | A kind of the natural of Pu'er tea compressed tea is aged method of growing dim |
| CN108029799A (en) * | 2017-11-22 | 2018-05-15 | 广西大学 | A kind of jasmine tea preparation method of bulk bacteria floating |
| CN108029829A (en) * | 2017-12-29 | 2018-05-15 | 中南林业科技大学 | A kind of paper mulberry golden flower tea preparation methods |
| CN108029829B (en) * | 2017-12-29 | 2021-05-11 | 中南林业科技大学 | Preparation method of paper mulberry golden camellia |
| CN108850296A (en) * | 2018-07-26 | 2018-11-23 | 陈殷 | A kind of preparation method being sustained six fort tea |
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| CN115251203A (en) * | 2022-07-27 | 2022-11-01 | 中华全国供销合作总社济南果品研究所 | Application and method of Xie Washi aspergillus in fermentation preparation of peony petal tea |
| CN115251203B (en) * | 2022-07-27 | 2024-07-05 | 中华全国供销合作总社济南果品研究所 | Application of Xie Washi aspergillus in preparing peony petal tea by fermentation and method |
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