CN106483099B - Based on tunable filter and the cascade optical biosensor of Michelson's interferometer - Google Patents
Based on tunable filter and the cascade optical biosensor of Michelson's interferometer Download PDFInfo
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- CN106483099B CN106483099B CN201610872896.1A CN201610872896A CN106483099B CN 106483099 B CN106483099 B CN 106483099B CN 201610872896 A CN201610872896 A CN 201610872896A CN 106483099 B CN106483099 B CN 106483099B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
- G01N2021/458—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods using interferential sensor, e.g. sensor fibre, possibly on optical waveguide
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Abstract
The invention discloses one kind to be based on tunable filter and the cascade optical biosensor of Michelson's interferometer, it includes wideband light source, tunable periodic filter, Michelson's interferometer and detector；The light of wideband light source enters Michelson's interferometer after tunable filter, and the molecule film that can adsorb measured matter in solution to be measured has been modified in the reflection end face of one of arm of Michelson's interferometer, and contacts with solution to be measured, as measurement arm；The reflection end face of another arm not modified biological molecular film, and contacted with solution to be measured, as reference arm；The output end of Michelson's interferometer is connected with detector.The present invention is using wideband light source and the periodic filter combination of low cost as input light source, laser without high wavelength stability, biomolecule is film modified on the glass substrate, structure is simple, only need probe power change information, without measuring wavelength information, the cost of manufacture of sensor is greatly reduced.
Description
Technical field
The present invention relates to a kind of optical sensors, more particularly to one kind to be based on tunable filter and Michelson's interferometer grade
The optical biosensor of connection.
Background technique
Biosensor has highly important application in biological detection, chemical analysis and environmental monitoring field.Low cost, height
Sensitivity and real-time online measuring are several technological difficulties of bio-sensing.Currently, most biosensor all will be biological
The variation of concentration is converted to electric signal and is detected, and detection time is longer, is unable to real-time online measuring.Unmarked optical bio passes
Sensor be uniquely can directly detect biomolecule reaction instrument, can quickly, monitor reaction process in real time, have it is sensitive
A series of advantages such as degree is high, anti-electromagnetic interference capability is strong.Currently, emerged in multitude based on optical biosensor technique, such as: it is glimmering
Light spectral technique, Raman diffused light spectral technology, absorption spectroscopy techniques and the refractive index Detection Techniques based on evanescent wave.However,
The shortcomings that optical bio sensing being largely commercialized at present, is that device volume is big, and expensive, required sample size is also big.
It is provided by the invention to be based on tunable periodic filter and the cascade unmarked optical bio of Michelson's interferometer
Sensor by tunable periodic filter with and Michelson's interferometer constitute, directly made in glass substrate or fiber end face
Molecule film is not necessarily to plated film, and production is simple, low in cost.The present invention penetrates the signal of solution to be measured without testing, therefore can
Big, even opaque liquid is absorbed with test.Simultaneously as phase information is extracted from interference signal, not by the wave of light source
Dynamic and detector sensitivity influence, and do not influenced by solution refractive index to be measured.
Summary of the invention
In view of the deficiencies of the prior art, the object of the present invention is to provide it is a kind of it is low in cost, structure is simple, high sensitivity
Technical solution based on the cascade unmarked optical biosensor of tunable periodic filter and Michelson's interferometer.This hair
The combination of the bright wideband light source and filter for using low cost is as input light source, receiver of the detector as output light.When
After measured matter is adsorbed on molecule film in solution to be measured, molecule film is caused to thicken, leads to Michelson's interferometer
Arm light path is measured to increase.Measured matter absorption front and back is recorded, the interference optical power that detector receives is with tunable filter spectrum
The information of scan variations, after Fourier transformation, available phase change information, the thickness for extrapolating molecule film becomes
Change, to obtain measured matter content information in solution to be measured.
The technical solution used to solve the technical problems of the present invention is that: one kind being based on tunable filter and Michelson interference
The cascade optical biosensor of instrument, including wideband light source, tunable periodic filter, Michelson's interferometer and detector；
The light of wideband light source enters Michelson's interferometer after tunable periodic filter；The Michelson's interferometer is wherein
The reflection end face modification of one arm can adsorb the molecule film of measured matter in solution to be measured, and contact with solution to be measured,
As measurement arm；The reflection end face of another arm not modified biological molecular film, and contacted with solution to be measured, as reference arm；
The output end of Michelson's interferometer is connected with detector.
Further, the refractive index phase of the refractive index of the biomolecule membrane material and the reflection face material being modified
Together.
Further, by adjusting two brachiums of the Michelson's interferometer, so that Michelson steller interferometer output spectra
The Free Spectral Range of line is identical as the Free Spectral Range of tunable periodic filter output spectrum；When tested in solution to be measured
After substance is adsorbed on molecule film, molecule film is caused to thicken, the measurement arm light path of Michelson's interferometer is caused to increase.
Further, the tunable periodic filter can be Fabry-Perot cavity；Either Mach-Zehnder
Interferometer；Either ring resonator；Or micro- disk resonant cavity；The position of the tunable periodic filter output spectral line resonance peak
Setting can be moved by changing the cavity length of filter.
Further, the Michelson's interferometer can be is made of the discrete device based on space optics；Either
Structure based on optical fiber is constituted；Either constituted based on integrated optical wave guide device.
The invention has the advantages that: optical biosensor of the present invention can utilize wideband light source, be not necessarily to test light
Spectrum information does not need spectrometer, can reduce the volume of sensor.In sheet glass, waveguide or fiber end face modified biological point
Sub- film greatly reduces the cost of sensor without the sensitive zones plating metal or deielectric-coating in sensor.Testing signal is
The reflecting interference signal of sensitive zones, therefore do not have to consider absorption of the light in testing liquid.Pass through test Michelson's interference
The phase change of instrument pickup arm obtains molecule film thickness change information.Because phase information is extracted from interference signal, no
Fluctuation and detector sensitivity by light source are influenced, and are not influenced by solution refractive index to be measured.
Detailed description of the invention
Fig. 1 is the structure based on tunable filter and the cascade optical biosensor of Michelson's interferometer in the present invention
Schematic diagram；
Fig. 2 is output spectrum of the tunable filter in initial position δ L=0 and δ L=534.5nm；
Fig. 3 be Michelson's interferometer before adsorb test substance δ h=0 and adsorb test substance after δ h=267.2nm
Output spectrum；
Fig. 4 is when tunable periodic filter resonance cavity length is initial position δ L=0, and test substance absorption front and back can
Spectrum is exported after tunable filter and Michelson's interferometer cascade；
Fig. 5 is that the optical power that detector receives before and after test substance adsorbs changes song with tunable filter cavity length
Line.
Fig. 6 (a) is the spectrogram for the interference light signal Fourier transformation that detector receives.
Fig. 6 (b) is the phase diagram for the interference light signal Fourier transformation that detector receives.
Fig. 7 is the obtained phase information of Fourier transformation with biomolecule film thickness change figure.
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples.
Fig. 1 is first embodiment schematic diagram of the invention.It includes: a wideband light source (1), an adjustable period
Property filter (2), a Michelson's interferometer (3), a detector (5).Wherein the one of the Michelson's interferometer (3)
The molecule film (6) that can adsorb measured matter in solution to be measured (4) has been modified in the reflection end face (32) of a arm (31), and with
Solution (4) contact to be measured, as measurement arm (31)；The reflection end face (34) of another arm (33) not modified biological molecular film,
And contacted with solution to be measured (4), as reference arm；The output end (35) of Michelson's interferometer is connected with detector (5).
Assuming that wideband light source (1) is flat in 1.53 μm of -1.57 μm of spectrum, the corresponding distribution of light intensity of each wavelength is
Unit 1.If tunable periodic filter (2) is made of integrated light guide ring resonator, straight wave guide and annular are output and input
The cross-coupling coefficient of resonant cavity is 0.5, and cavity length is L=400 μm, the effective refractive index n of resonant cavity1It is 1.45.
The transmittance graph T of tunable periodic filter (2) when resonant cavity is in initial position δ L=0 and δ L=534.5nm1Such as figure
Shown in 2.It can be seen in fig. 2 that because resonant cavity is long, when cavity length changes, the free light of resonant cavity
There is no significant changes for spectral limit, but significant movement has occurred in resonance wavelength position.
Michelson's interferometer is to constitute (splitting ratio 50%:50%) by 2 × 2 fiber coupler in this example, can
Periodic filter (2) are adjusted to be connected with an input port of fiber coupler, two arms (31) of Michelson's interferometer
(33) be respectively fiber coupler two output optical fibres, so reflection end face (32) and (34) be fiber end face, optical fiber coupling
Another input terminal of clutch is connected with detector (5).Assuming that the refraction of optical fiber equivalent refractive index and molecule film
Rate n1It is 1.45；The refractive index n of solution to be measured2It is 1.33.
It is R in the reflectivity of fiber end face or biomolecule film surface and solution to be measured
Because the end face reflection rate of two arms of Michelson's interferometer is identical, the output spectra of Michelson's interferometer
Line is T2
WhereinH is the optical path difference of Michelson's interferometer (3) two-arm, to make Michelson steller interferometer (3)
Export the Free Spectral Range h=800 μ identical as the Free Spectral Range of tunable periodic filter (2) output spectrum of spectral line
M, δ h are that molecule film adsorbs caused biomolecule film thickness change after measured matter, and λ is lambda1-wavelength.Fig. 3 is provided
The output spectral line T of Michelson's interferometer δ h=267.2nm after δ h=0 and absorption before absorption2.Because two arms is anti-
Penetrate that rate is identical, so the minimum value of transmissivity can be equal to 0.As can be seen from Fig. 3, because of the light path of Michelson's interferometer two-arm
Difference is long, so there is no obviously becoming for the Free Spectral Range of Michelson's interferometer when test substance adsorbs
Change, but interferes extremum wavelengths position that significant movement has occurred.
Fig. 4 is when tunable filter cavity length is initial position δ L=0, and test substance is adsorbed fore-aft adjustable week
Spectrum is exported after phase property filter and Michelson's interferometer cascade.Before test substance is adsorbed, tunable periodic filtering
The maximum and minimum position of device and Michelson's interferometer are overlapped, and transmissivity is maximum after cascading at this time；In test substance
It is adsorbed rear δ h=267.2nm, the maximum of tunable periodic filter and the minimum position of Michelson's interferometer are overlapped,
The maximum position of the minimum of tunable periodic filter and Michelson's interferometer is overlapped, and transmissivity is most after cascading at this time
It is small.
When the resonant cavity variation range of tunable periodic filter is from -6 μm to 6 μm (assuming that scanning a cycle is used
12ms), it is adsorbed front and back in test substance, it is as shown in Figure 5 detects the optical power change received.It can be seen that when test substance is inhaled
When attached, movement is had occurred in optical power change curve, and optical power change curve is carried out Fourier transformation, and the phase for calculating dominant frequency becomes
Change information, so that it may measure biomolecule film thickness change after absorption test substance.
Fig. 6 is that test substance is adsorbed front and back, the Fourier transformation information for the optical power change curve that detector receives.
When test substance is adsorbed front and back, the position of basic frequency is constant (in 916.6Hz), but phase is inhaled with molecule film
The thickness change of attached test substance and change.As shown in fig. 7, phase is from π to pi/2, then to 0, adsorption thickness is respectively corresponded from first
Beginning state 0nm arrives 133.6nm, arrives 267.2nm.The every increase 1nm of the thickness of molecule film, phase reduce 0.0118rad.When
When the power of incident light source changes or sample refractive index changes, influence is only that detector receives
Power fluctuation, but its phase is not exposed to influence, therefore still can measure measured matter content.
Above-described embodiment is used to illustrate the present invention, rather than limits the invention.In spirit of the invention and
In scope of protection of the claims, to any modifications and changes that the present invention makes, protection scope of the present invention is both fallen within.
Claims (5)
1. one kind is based on tunable filter and the cascade optical biosensor of Michelson's interferometer, which is characterized in that including
Wideband light source (1), tunable periodic filter (2), Michelson's interferometer (3) and detector (5)；The light of wideband light source (1)
Enter Michelson's interferometer (3) after tunable periodic filter (2)；Wherein the one of the Michelson's interferometer (3)
Reflection end face (32) modification of a arm (31) can adsorb the molecule film (6) of measured matter in solution to be measured (4), and with to
Solution (4) contact is surveyed, as measurement arm (31)；The reflection end face (34) of another arm (33) not modified biological molecular film, and
It is contacted with solution to be measured (4), as reference arm；The output end (35) of Michelson's interferometer (3) is connected with detector (5).
2. according to claim 1 a kind of based on tunable filter and the cascade optical bio sensing of Michelson's interferometer
Device, which is characterized in that the refractive index of the refractive index of molecule film (6) material and reflection end face (32) material being modified
It is identical.
3. according to claim 1 a kind of based on tunable filter and the cascade optical bio sensing of Michelson's interferometer
Device, which is characterized in that by adjusting two brachiums of the Michelson's interferometer (3), so that Michelson's interferometer (3) is defeated
The Free Spectral Range of spectral line is identical as the Free Spectral Range of tunable periodic filter (2) output spectrum out；When to be measured molten
After measured matter is adsorbed on molecule film (6) in liquid (4), molecule film is caused to thicken, leads to Michelson's interferometer (3)
Measurement arm light path increase.
4. according to claim 1 a kind of based on tunable filter and the cascade optical bio sensing of Michelson's interferometer
Device, which is characterized in that the tunable periodic filter (2) can be Fabry-Perot cavity；Either Mach-Zehnder
Interferometer；Either ring resonator；Or micro- disk resonant cavity；The tunable periodic filter (2) exports spectral line resonance peak
Position can by change filter cavity length be moved.
5. according to claim 1 a kind of based on tunable filter and the cascade optical bio sensing of Michelson's interferometer
Device, which is characterized in that the Michelson's interferometer (3) can be to be made of the discrete device based on space optics；Either
Structure based on optical fiber is constituted；Either constituted based on integrated optical wave guide device.
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| CN111164417B (en) * | 2017-09-01 | 2023-06-23 | 迈普有限公司 | Photon interferometer based sensing |
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| US6330062B1 (en) * | 1999-04-30 | 2001-12-11 | Wisconsin Alumni Research Foundation | Fourier transform surface plasmon resonance adsorption sensor instrument |
| CN103134436A (en) * | 2013-02-04 | 2013-06-05 | 博奥生物有限公司 | Full-spectrum super-resolution measuring method and label-free biomolecule measurement system |
| CN104359868A (en) * | 2014-11-07 | 2015-02-18 | 中国计量学院 | Tilted fiber bragg grating surface plasma resonance biosensor based on M-Z interference |
| CN104568839A (en) * | 2014-12-17 | 2015-04-29 | 浙江大学 | Biosensor based on cascade connection of optical resonant cavity and reflective polarization converter |
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Patent Citations (4)
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|---|---|---|---|---|
| US6330062B1 (en) * | 1999-04-30 | 2001-12-11 | Wisconsin Alumni Research Foundation | Fourier transform surface plasmon resonance adsorption sensor instrument |
| CN103134436A (en) * | 2013-02-04 | 2013-06-05 | 博奥生物有限公司 | Full-spectrum super-resolution measuring method and label-free biomolecule measurement system |
| CN104359868A (en) * | 2014-11-07 | 2015-02-18 | 中国计量学院 | Tilted fiber bragg grating surface plasma resonance biosensor based on M-Z interference |
| CN104568839A (en) * | 2014-12-17 | 2015-04-29 | 浙江大学 | Biosensor based on cascade connection of optical resonant cavity and reflective polarization converter |
Non-Patent Citations (1)
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| 基于干涉光谱分析的生物传感技术研究;戴恒;《中国优秀硕士学位论文全文数据库 信息科技辑》;20110615(第06期);第I140-169页 |
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